ABSTRACT
Oscillatoxins (OTXs) and aplysiatoxins are biosynthetically related polyketides produced by marine cyanobacteria. We previously developed a synthetic route to phenolic O-methyl analogs of OTX-D and 30-methyl-OTX-D during collective synthesis of these natural products. According to our synthetic strategy, we achieved total synthesis of OTX-D, 30-methyl-OTX-D, OTX-E, and OTX-F by deprotecting the O-methyl group in an earlier intermediate, and determined their biological activities. Although OTX-D and 30-methyl-OTX-D have been reported to show antileukemic activity against L1210 cell line, we found that their cytotoxicity in vitro against this cell line is relatively weak (IC50: 29-52 µm). In contrast, OTX-F demonstrated cell line-selective antiproliferative activity against DMS-114 lung cancer cells, which implies that OTXs target as yet unknown target molecules as part of this unique activity.
Subject(s)
Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Bacterial Toxins/chemical synthesis , Bacterial Toxins/pharmacology , Antineoplastic Agents/chemistry , Bacterial Toxins/chemistry , Cell Line, Tumor , Cell Proliferation/drug effects , Chemistry Techniques, Synthetic , HumansABSTRACT
We report the functional synthesis and quantification of membrane proteins-α-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli-at the stabilized droplet interface bilayer using an in vitro transcription-translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.
Subject(s)
Bacterial Toxins/chemical synthesis , Cell-Free System , Escherichia coli Proteins/chemical synthesis , Hemolysin Proteins/chemical synthesis , Lipid Bilayers , Membrane Proteins/chemical synthesisABSTRACT
Fresh water cyanobacterial algal blooms represent a major health risk because these organisms produce cylindrospermopsin, a toxic, structurally complex, zwitterionic uracil-guanidine alkaloid recognized by the EPA as a dangerous drinking water contaminant. At present, the ability to detect and quantify the presence of cylindrospermospin in water samples is severely hampered by the lack of an isotopically labeled standard for analytical mass spectrometry. Herein, we present a concise, scaled total synthesis of 15N cylindrospermosin from 15N ammonium chloride, which leverages a unique stereoselective intramolecular double conjugate addition step to assemble the tricyclic guanidine core. In addition to providing the first pure isotopically labeled probe for precise quantification of this potent biotoxin in fresh water sources, our results demonstrate how unique constraints associated with isotope incorporation compel novel solutions to synthesis design.
Subject(s)
Ammonium Chloride/chemistry , Bacterial Toxins/chemical synthesis , Cyanobacteria/chemistry , Fresh Water/analysis , Uracil/analogs & derivatives , Water Pollutants, Chemical/analysis , Alkaloids , Bacterial Toxins/chemistry , Cyanobacteria Toxins , Environmental Monitoring , Molecular Structure , Nitrogen Isotopes , Uracil/chemical synthesis , Uracil/chemistryABSTRACT
Staphylococcus aureus produces peptide toxins that it uses to respond to environmental cues. We previously characterized PepA1, a peptide toxin from S. aureus, that induces lytic cell death of both bacterial and host cells. That led us to suggest that PepA1 has an antibacterial activity. Here, we demonstrate that exogenously provided PepA1 has activity against both Gram-positive and Gram-negative bacteria. We also see that PepA1 is significantly hemolytic, thus limiting its use as an antibacterial agent. To overcome these limitations, we converted PepA1 into nonhemolytic derivatives. Our most promising derivative is a cyclic heptapseudopeptide with inconsequential toxicity to human cells, enhanced stability in human sera, and sharp antibacterial activity. Mechanistically, linear and helical PepA1 derivatives form pores at the bacterial and erythrocyte surfaces, while the cyclic peptide induces bacterial envelope reorganization, with insignificant action on the erythrocytes. Our work demonstrates that bacterial toxins might be an attractive starting point for antibacterial drug development.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacokinetics , Staphylococcus aureus/metabolism , Amino Acid Sequence , Anti-Bacterial Agents/metabolism , Bacterial Toxins/chemical synthesis , Bacterial Toxins/metabolism , Bacterial Toxins/pharmacology , Escherichia coli/drug effects , Hemolytic Agents/chemical synthesis , Hemolytic Agents/metabolism , Hemolytic Agents/pharmacology , Humans , Microbial Sensitivity Tests , Molecular Sequence Data , Peptides, Cyclic/chemical synthesis , Peptides, Cyclic/pharmacology , Protein Engineering , Staphylococcus aureus/chemistryABSTRACT
Cylindrospermopsin (CYN) is a cyanobacterial toxin associated with human and animal poisonings. Due to its toxicity in combination with its widespread occurrence, the development of reliable methods for selective, sensitive detection and accurate quantification is mandatory. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis using stable isotope dilution analysis (SIDA) represents an ideal tool for this purpose. U-[(15)N5]-CYN was synthesized by culturing Aphanizomenon flos-aquae in Na(15)NO3-containing cyanobacteria growth medium followed by a cleanup using graphitized carbon black columns and mass spectrometric characterization. Subsequently, a SIDA-LC-MS/MS method for the quantification of CYN in freshwater and Brassica matrices was developed showing satisfactory performance data. The recovery ranged between 98 and 103 %; the limit of quantification was 15 ng/L in freshwater and 50 µg/kg dry weight in Brassica samples. The novel SIDA was applied for CYN determination in real freshwater samples as well as in kale and in vegetable mustard exposed to toxin-containing irrigation water. Two of the freshwater samples taken from German lakes were found to be CYN-contaminated above limit of quantification (17.9 and 60.8 ng/L). CYN is systemically available to the examined vegetable species after exposure of the rootstock leading to CYN mass fractions in kale and vegetable mustard leaves of 15.0 µg/kg fresh weight and 23.9 µg/kg fresh weight, respectively. CYN measurements in both matrices are exemplary for the versatile applicability of the developed method in environmental analysis.
Subject(s)
Bacterial Toxins/chemistry , Brassica/chemistry , Fresh Water/analysis , Indicator Dilution Techniques , Marine Toxins/chemistry , Microcystins/chemistry , Tandem Mass Spectrometry/methods , Uracil/analogs & derivatives , Alkaloids , Bacterial Toxins/chemical synthesis , Bacterial Toxins/metabolism , Brassica/microbiology , Cyanobacteria/metabolism , Cyanobacteria Toxins , Fresh Water/microbiology , Indicator Dilution Techniques/standards , Isotope Labeling , Marine Toxins/chemical synthesis , Marine Toxins/metabolism , Microcystins/chemical synthesis , Microcystins/metabolism , Nitrogen Isotopes/chemistry , Reference Standards , Tandem Mass Spectrometry/instrumentation , Tandem Mass Spectrometry/standards , Uracil/chemical synthesis , Uracil/chemistry , Uracil/metabolismABSTRACT
The human pathogen Staphylococcus aureus is renowned for the rapid colonization of contaminated wounds, medical implants, and food products. Nevertheless, little is known about the mechanisms that allow S. aureus to colonize the respective wet surfaces. The present studies were therefore aimed at identifying factors used by S. aureus cells to spread over wet surfaces, starting either from planktonic or biofilm-associated states. Through proteomics analyses we pinpoint phenol-soluble modulins (PSMs) as prime facilitators of the spreading process. To dissect the roles of the eight PSMs produced by S. aureus, these peptides were chemically synthesized and tested in spreading assays with different psm mutant strains. The results show that PSMα3 and PSMγ are the strongest facilitators of spreading both for planktonic cells and cells in catheter-associated biofilms. Compared to the six other PSMs of S. aureus, PSMα3 and PSMγ combine strong surfactant activities with a relatively low overall hydropathicity. Importantly, we show that PSM-mediated motility of S. aureus facilitates the rapid colonization of wet surfaces next to catheters and the colonization of fresh meat.
Subject(s)
Bacterial Toxins/metabolism , Environmental Microbiology , Meat/microbiology , Staphylococcus aureus/growth & development , Staphylococcus aureus/metabolism , Bacterial Toxins/chemical synthesis , Biofilms/growth & development , Catheters/microbiology , Humans , Staphylococcus aureus/physiology , Surface-Active Agents/metabolismABSTRACT
ST-based lipopeptide vaccine candidates were constructed in which ST was chemically synthesized and folded into the correct conformation prior to ligation to a module containing a T-helper cell epitope (T(H)) and the Toll-like receptor 2 (TLR2) agonist, S-[2,3-bis(palmitoyloxy)propyl]cysteine (P2C). Two different chemistries, thioether-based and oxime-based, were then used to ligate ST to the lipidated T(H) epitope. The enterotoxic activity of synthetic ST and the ST-based lipopeptide vaccines was determined in mice followed by an evaluation of immunological efficacy. The importance of the fine detail in chemical composition used in vaccine design was demonstrated by the findings that (i) the oxime-based vaccine exhibited little or no toxicity but the thioether-based vaccine, exhibited residual toxicity in suckling mice, (ii) although each of the synthetic vaccines generated specific anti-ST antibodies, it was the low titer antibodies induced by the oxime-based vaccine that demonstrated better neutralizing activity suggesting that the chemical linkage also affects the specificity of antibodies, (iii) the geometric arrangement of ST within a vaccine can profoundly affect the specificity and biological function of the antibodies that are elicited, and (iv) the lipopeptide-based ST vaccine candidate assembled using oxime chemistry induced a better neutralizing antibody response to ST when administered by the mucosal (intranasal) route.
Subject(s)
Adjuvants, Immunologic/chemistry , Bacterial Toxins/immunology , Enterotoxins/immunology , Escherichia coli Vaccines/immunology , Lipopeptides/immunology , Administration, Intranasal , Animals , Antibodies, Bacterial/blood , Antibodies, Neutralizing/blood , Antibody Specificity , Bacterial Toxins/chemical synthesis , Enterotoxigenic Escherichia coli/immunology , Enterotoxins/chemical synthesis , Epitopes, T-Lymphocyte/chemistry , Epitopes, T-Lymphocyte/immunology , Escherichia coli Proteins , Escherichia coli Vaccines/chemical synthesis , Female , Mice , Mice, Inbred BALB C , Neutralization Tests , Oximes/immunology , Toll-Like Receptor 2/agonists , Vaccines, Synthetic/immunologyABSTRACT
Mycolactones are complex macrolides responsible for a severe necrotizing skin disease called Buruli ulcer. Deciphering their functional interactions is of fundamental importance for the understanding, and ultimately, the control of this devastating mycobacterial infection. We report herein a diverted total synthesis approach of mycolactones analogues and provide the first insights into their structure-activity relationship based on cytopathic assays on L929 fibroblasts. The lowest concentration inducing a cytopathic effect was determined for selected analogues, allowing a clear picture to emerge by comparison with the natural toxins.
Subject(s)
Bacterial Toxins/chemical synthesis , Buruli Ulcer/chemically induced , Macrolides/chemical synthesis , Animals , Bacterial Toxins/chemistry , Bacterial Toxins/pharmacology , Buruli Ulcer/microbiology , Buruli Ulcer/pathology , Fibroblasts/drug effects , Macrolides/chemistry , Macrolides/pharmacology , Mice , Molecular Structure , Mycobacterium Infections/pathology , Mycobacterium ulcerans/chemistry , Structure-Activity RelationshipABSTRACT
The total synthesis of the mycobacterial toxins mycolactonesâ A/B (1 a/b) has been accomplished based on a strategy built around the construction of the mycolactone core through ring-closing metathesis. By employing the Grubbs second-generation catalyst, the 12-membered core macrocycle of mycolactones, with a functionalized C2 handle attached to C11, was obtained in 60-80 % yield. The C-linked upper side chain (comprising C12-C20) was completed by a highly efficient modified Suzuki coupling between C13 and C14, while the attachment of the C5-O-linked polyunsaturated acyl side chain was achieved by Yamaguchi esterification. Surprisingly, a diene containing a simple isopropyl group attached to C11 could not be induced to undergo ring-closing metathesis. By employing fluorescence microscopy and flow cytometry techniques, the synthetic mycolactonesâ A/B (1 a/b) were demonstrated to display similar apoptosis-inducing and cytopathic effects as mycolactonesâ A/B extracted from Mycobacterium ulcerans. In contrast, a simplified analogue with truncated upper and lower side chains was found to be inactive.
Subject(s)
Bacterial Toxins/chemical synthesis , Animals , Apoptosis , Bacterial Toxins/chemistry , Catalysis , Macrolides , Mice , Molecular Structure , Mycobacterium ulcerans/chemistryABSTRACT
Unprecedentedly efficient and highly (≥98 %) stereoselective syntheses of mycolactones A and B side chains relied heavily on Pd-catalyzed alkenylation (Negishi version) and were completed in 11 longest linear steps from ethyl (S)-3-hydroxybutyrate in 12% and 11% overall yield, respectively, roughly corresponding to an average of 82% yield per step. The synthesis of mycolactone core was realized by using Pd-catalyzed alkenyl-allyl coupling and an epoxide-opening reaction with a trialkylalkenylaluminate as key steps. Fully hydroxy-protected mycolactones A and B of ≥98% isomeric purity were synthesized successfully for the first time. However, unexpected 4:3-5:4 inseparable mixtures of mycolactones A and B were obtained upon deprotection.
Subject(s)
Alkenes/chemistry , Bacterial Toxins/chemistry , Bacterial Toxins/chemical synthesis , Lactones/chemistry , Lactones/chemical synthesis , Catalysis , Macrolides , Molecular Structure , StereoisomerismABSTRACT
Buruli ulcer is a severe and devastating skin disease caused by Mycobacterium ulcerans infection, yet it is one of the most neglected diseases. The causative toxin, referred to as mycolactone A/B, was isolated and characterized as a polyketide-derived macrolide in 1999. The current status of the mycolactone chemistry is described, highlighting the stereochemistry assignment of mycolactone A/B; total synthesis; the structure determination of mycolactone congeners from the human pathogen M. ulcerans, the frog pathogen Mycobacterium liflandii, and the fish pathogen Mycobacterium marinum; the structural diversity in the mycolactone class of natural products; the highly sensitive detection/structure-analysis of mycolactones; and some biological activity.
Subject(s)
Bacterial Toxins/chemistry , Bacterial Toxins/chemical synthesis , Buruli Ulcer/microbiology , Mycobacterium ulcerans/chemistry , Animals , Anura , Bacterial Toxins/toxicity , Buruli Ulcer/chemically induced , Buruli Ulcer/pathology , Fish Diseases/microbiology , Fish Diseases/pathology , Fishes , Guinea Pigs , Humans , Macrolides , Molecular Structure , Mycobacterium ulcerans/pathogenicityABSTRACT
The technology described here allows the chemical synthesis of vaccines requiring correctly folded epitopes and that contain difficult or long peptide sequences. The final self-adjuvanting product promotes strong humoral and/or cell-mediated immunity. A module containing common components of the vaccine (T helper cell epitope and the adjuvanting lipid moiety S-[2,3-bis(palmitoyloxy)propyl]cysteine) was assembled to enable a plug and play approach to vaccine assembly. The inclusion within the module of a chemical group with chemical properties complementary and orthogonal to a chemical group present in the target epitope allowed chemoselective ligation of the two vaccine components. The heat-stable enterotoxin of enterotoxigenic Escherichia coli that requires strict conformational integrity for biological activity and the reproductive hormone luteinizing hormone-releasing hormone were used as the target epitopes for the antibody vaccines. An epitope from the acid polymerase of influenza virus was used to assemble a CD8(+) T cell vaccine. Evaluation of each vaccine candidate in animals demonstrated the feasibility of the approach and that the type of immune response required, viz. antibody or cytotoxic T lymphocyte, dictates the nature of the chemical linkage between the module and target epitope. The use of a thioether bond between the module and target epitope had little or no adverse effect on antibody responses, whereas the use of a disulfide bond between the module and target epitope almost completely abrogated the antibody response. In contrast, better cytotoxic T lymphocyte responses were obtained when a disulfide bond was used.
Subject(s)
Adjuvants, Immunologic , Epitopes, T-Lymphocyte , Lipopeptides , Vaccines, Synthetic , Adjuvants, Immunologic/chemical synthesis , Adjuvants, Immunologic/pharmacology , Animals , Bacterial Toxins/chemical synthesis , Bacterial Toxins/immunology , Bacterial Toxins/pharmacology , CD8-Positive T-Lymphocytes/immunology , Enterotoxigenic Escherichia coli/chemistry , Enterotoxigenic Escherichia coli/immunology , Enterotoxins/chemical synthesis , Enterotoxins/immunology , Enterotoxins/pharmacology , Epitopes, T-Lymphocyte/immunology , Epitopes, T-Lymphocyte/pharmacology , Escherichia coli Proteins , Gonadotropin-Releasing Hormone/chemical synthesis , Gonadotropin-Releasing Hormone/immunology , Gonadotropin-Releasing Hormone/pharmacology , Lipopeptides/chemical synthesis , Lipopeptides/immunology , Lipopeptides/pharmacology , Mice , Mice, Inbred BALB C , Orthomyxoviridae/chemistry , Orthomyxoviridae/immunology , Vaccines, Synthetic/immunology , Vaccines, Synthetic/pharmacologyABSTRACT
Two approaches are presented for the synthesis of the macrolide core of the mycolactone polyketides. The first intertwines ring closing metathesis (RCM) within a two-step Julia olefination protocol, while the second intercepts the optimized routes of Kishi, thereby providing formal access to the mycolactones.
Subject(s)
Bacterial Toxins/chemistry , Bacterial Toxins/chemical synthesis , Alkenes , Macrolides , Mycobacterium/chemistryABSTRACT
Many bacterial toxins act by covalently altering molecular targets within the cytosol of mammalian cells and therefore must transport their catalytic moieties across a membrane. The Protective-Antigen (PA) moiety of anthrax toxin forms multimeric pores that transport the two enzymatic moieties, the Lethal Factor (LF) and the Edema Factor, across the endosomal membrane to the cytosol. The homologous PA-binding domains of these enzymes contain N-terminal segments of highly charged amino acids that are believed to enter the pore and initiate N- to C-terminal translocation. Here we describe a semisynthesis platform that allows chemical control of this segment in LF(N), the PA-binding domain of LF. Semisynthetic LF(N) was prepared in milligram quantities by native chemical ligation of synthetic LF(N)(14-28)alphathioester with recombinant N29C-LF(N)(29-263) and compared with two variants containing alterations in residues 14-28 of the N-terminal region. The properties of the variants in blocking ion conductance through the PA pore and translocating across planar phospholipid bilayers in response to a pH gradient were consistent with current concepts of the mechanism of polypeptide translocation through the pore. The semisynthesis platform thus makes new analytical approaches available to investigate the interaction of the pore with its substrates.
Subject(s)
Anthrax/metabolism , Antigens, Bacterial/chemistry , Antigens, Bacterial/metabolism , Bacillus anthracis/chemistry , Bacillus anthracis/metabolism , Bacterial Toxins/chemistry , Bacterial Toxins/metabolism , Amino Acid Sequence , Bacterial Toxins/chemical synthesis , Ions/metabolism , Lipid Bilayers/metabolism , Models, Molecular , Molecular Sequence Data , Protein Folding , Protein Structure, TertiaryABSTRACT
Antimicrobial peptides serve as a first line of innate immune defense against invading organisms such as bacteria and viruses. In this study, we hypothesized that peptides produced by a normal microbial resident of human skin, Staphylococcus epidermidis, might also act as an antimicrobial shield and contribute to normal defense at the epidermal interface. We show by circular dichroism and tryptophan spectroscopy that phenol-soluble modulins (PSMs) gamma and delta produced by S. epidermidis have an alpha-helical character and a strong lipid membrane interaction similar to mammalian AMPs such as LL-37. Both PSMs directly induced lipid vesicle leakage and exerted selective antimicrobial action against skin pathogens such as Staphylococcus aureus. PSMs functionally cooperated with each other and LL-37 to enhance antimicrobial action. Moreover, PSMs reduced Group A Streptococcus (GAS) but not the survival of S. epidermidis on mouse skin. Thus, these data suggest that the production of PSMgamma and PSMdelta by S. epidermidis can benefit cutaneous immune defense by selectively inhibiting the survival of skin pathogens while maintaining the normal skin microbiome.
Subject(s)
Bacterial Toxins/pharmacology , Keratinocytes/microbiology , Staphylococcus epidermidis/metabolism , Streptococcal Infections/drug therapy , Streptococcus pyogenes , Animals , Antimicrobial Cationic Peptides/metabolism , Bacterial Toxins/chemical synthesis , Cell Membrane Permeability , Cells, Cultured , Circular Dichroism , Epidermal Cells , Epidermis/immunology , Epidermis/microbiology , Humans , Keratinocytes/cytology , Keratinocytes/immunology , Lipid Bilayers/metabolism , Membranes, Artificial , Mice , Mice, Inbred C57BL , Protein Structure, Secondary , Staphylococcal Skin Infections/drug therapy , Staphylococcal Skin Infections/immunology , Staphylococcal Skin Infections/microbiology , Staphylococcus aureus , Streptococcal Infections/immunology , Streptococcal Infections/microbiology , SymbiosisABSTRACT
One of the most important requirements for non-viral gene delivery systems is the ability to mediate high levels of gene expression with low toxicity. After the DNA/vector complexes are taken up by cells through endocytosis, DNA is typically contained within the endocytic compartments and rapidly degraded due to the low pH and hydrolytic enzymes within endosomes and lysosomes, limiting its accessibility to the cytosol and ultimately to the nucleus. In this study, the endosomolytic protein listeriolysin O (LLO) from the intracellular pathogen Listeria monocytogenes was conjugated with polyethylenimine (PEI) of average molecular weight 25 kDa (PEI25) via a reversible disulfide bond (LLO-s-s-PEI), and incorporated into plasmid DNA condensed with disulfide-crosslinked low molecular weight PEI 1.8 kDa (PEI1.8). We have investigated and demonstrated that high gene transfection efficiency, which is comparable to that by the most commonly used PEI25, can be achieved by reversibly crosslinking low molecular weight PEI (PEI1.8) using disulfide bonds, with greatly reduced cytotoxicity of the PEI. The reversible incorporation of LLO into the DNA condensates of PEI, through the use of the synthesized LLO-s-s-PEI conjugate, further enhances the transfection efficiency beyond that of DNA condensates with disulfide-crosslinked PEI1.8 alone.
Subject(s)
Bacterial Toxins/toxicity , Gene Transfer Techniques , Heat-Shock Proteins/toxicity , Hemolysin Proteins/toxicity , Polyethyleneimine/chemical synthesis , Polyethyleneimine/toxicity , Bacterial Toxins/chemical synthesis , Bacterial Toxins/chemistry , Bacterial Toxins/genetics , Cell Survival/drug effects , Cells, Cultured , Cross-Linking Reagents/chemical synthesis , Cross-Linking Reagents/chemistry , DNA/administration & dosage , DNA/chemical synthesis , DNA/genetics , Disulfides/chemical synthesis , Disulfides/chemistry , Erythrocyte Membrane/drug effects , Gene Expression , Heat-Shock Proteins/chemical synthesis , Heat-Shock Proteins/chemistry , Heat-Shock Proteins/genetics , Hemolysin Proteins/chemical synthesis , Hemolysin Proteins/chemistry , Hemolysin Proteins/genetics , Humans , Luciferases/genetics , Molecular Weight , Plasmids/genetics , Polyethyleneimine/chemistry , TransfectionABSTRACT
Guanylyl cyclase C (GC-C), universally overexpressed on primary and metastatic colorectal carcinoma cells, is activated by endogenous ligands, guanylin, and uroguanylin, and by exogenous 18-residue heat-stable enterotoxins (STa) produced by diarrheagenic bacteria. Two 12-residue STa analogs with alternate combinations of two interlocked disulfide bonds, peptides 3 and 6, were synthesized by orthogonal solid phase synthesis routes. Peptides 3 and 6 bound GC-C with a rank order potency of STa > peptide 3 > peptide 6. Peptides 3 and 6 behaved as agonists in stimulating cGMP production. The results reveal that the toxic domain of STa can be reduced to 12 amino acids.
Subject(s)
Bacterial Toxins/metabolism , Enterotoxins/metabolism , Guanylate Cyclase/metabolism , Peptides/metabolism , Receptors, Peptide/metabolism , Amino Acid Sequence , Animals , Bacterial Toxins/chemical synthesis , Bacterial Toxins/genetics , Cell Line, Tumor , Cells, Cultured , Colorectal Neoplasms/diagnosis , Colorectal Neoplasms/enzymology , Colorectal Neoplasms/microbiology , Disulfides/metabolism , Drug Delivery Systems , Enterotoxins/chemical synthesis , Enterotoxins/genetics , Escherichia coli Infections/diagnosis , Escherichia coli Infections/enzymology , Escherichia coli Proteins , Guanylate Cyclase/chemical synthesis , Humans , Intestinal Mucosa/enzymology , Intestinal Mucosa/microbiology , Mice , Molecular Sequence Data , Peptides/administration & dosage , Peptides/chemical synthesis , Peptides/genetics , Receptors, Enterotoxin , Receptors, Guanylate Cyclase-CoupledABSTRACT
The undecenolide core of mycolactone was synthesized by ring-closing metathesis and the structure confirmed using single-crystal X-ray diffraction techniques.
Subject(s)
Bacterial Toxins/chemical synthesis , Bacterial Toxins/chemistry , Crystallography, X-Ray , Cyclization , Macrolides , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Conformation , Sensitivity and Specificity , StereoisomerismABSTRACT
A novel total synthesis of apratoxin A is described, with key steps including the assembly of its ketide segment through a D-proline-catalyzed direct aldol reaction and Oppolzer's anti aldol reaction and the preparation of its thiazoline unit in a biomimetic synthesis. An oxazoline analogue of apratoxin A has also been elaborated by a similar approach. This compound has a potency against HeLa cell proliferation only slightly lower than that of apratoxin A, whilst a C(40)-demethylated oxazoline analogue of apratoxin A displays a much lower cytotoxicity and the C(37)-epimer and C(37) demethylation product of this new analogue are inactive. These results suggest that the two methyl groups at C(37) and C(40) and the stereochemistry at C(37) are essential for the potent cellular activity of the oxazoline analogue of apratoxin A. Further biological analysis revealed that both synthetic apratoxin A and its oxazoline analogue inhibited cell proliferation by causing cell cycle arrest in the G1 phase.
Subject(s)
Antineoplastic Agents/chemical synthesis , Depsipeptides/chemical synthesis , Peptides, Cyclic/chemical synthesis , Antineoplastic Agents/pharmacology , Bacterial Toxins/chemical synthesis , Cell Cycle/drug effects , Cell Proliferation/drug effects , Depsipeptides/pharmacology , G1 Phase/drug effects , HeLa Cells , Humans , Methylation , Oxazoles , Peptides, Cyclic/pharmacology , Stereoisomerism , Structure-Activity RelationshipABSTRACT
[structure: see text] An ester dienolate [2,3]-Wittig rearrangement was utilized to access the alkylated citric acid skeleton 6 that is characteristic for the viridiofungins and other members of the alkyl citrate family of secondary natural products. The [2,3]-sigmatropic rearrangement of (Z,Z)-15 provided the rearrangement product (+/-)-syn-16 in moderate yield and with very good diastereoselectivity. A Julia-Kocienski olefination efficiently served to connect the polar head (+/-)-syn-26 with the lipophilic tail (32a-c) of the viridiofungins. Amide formation between the racemic viridiofungin precursors 35a-c and the enantiomerically pure amino acid L-tyrosine methyl ester followed by preparative reversed-phase HPLC provided the isopropyl dimethyl ester of viridiofungin A ((+)-39a), A2 ((+)-39b), and A4 ((+)-39c) as well as the nonnatural diastereomers (-)-38a-c.