ABSTRACT
Seven undescribed benzoate glycosides (1-7) and five known ones (8-12) were isolated from the rhizomes of Gentiana scabra Bge. Their structures were characterized by comprehensive NMR and MS spectroscopic data analysis. The lipid-lowering effects of these compounds were evaluated by measuring the triglyceride (TG) contents and intracellular lipid droplets (LDs) in oleic acid (OA)-treated HepG2 cells. The results showed that compounds 1, 5, 7, and 11 significantly reduced the TG content at 20 µM, and the Bodipy staining displayed that OA enhanced the levels of LDs in the cell, while these compounds reversed the lipid accumulation caused by OA. These findings provide a basis for further development and utilization of G. scabra as a natural source of potential lipid-lowering agents.
Subject(s)
Gentiana , Glycosides , Hypolipidemic Agents , Glycosides/pharmacology , Glycosides/chemistry , Glycosides/isolation & purification , Humans , Gentiana/chemistry , Hep G2 Cells , Hypolipidemic Agents/pharmacology , Hypolipidemic Agents/chemistry , Hypolipidemic Agents/isolation & purification , Benzoates/pharmacology , Benzoates/chemistry , Benzoates/isolation & purification , Molecular Structure , Oleic Acid/pharmacology , Oleic Acid/chemistry , Structure-Activity Relationship , Dose-Response Relationship, Drug , Triglycerides , Rhizome/chemistryABSTRACT
ABSTRACT Objective: To investigate the secondary metabolites from the cultures of Streptomyces sp CSDX076. Methods: The compounds were isolated using column chromatography and RP-18 medium-pressure liquid chromatography. Their structures were elucidated by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopic methods in combination with mass spectrometry experiments. Results: Four compounds were isolated from the cultures of Streptomyces sp CSDX076 and identified as aurantiamide benzoate, deoxytryptoquivaline, 2-acetyl-3,5-dihydroxyl-benzene acetic acid, and 2-acetyl-3,5-dihydroxyl-benzene ester. Conclusion: It was the first time that the four isolated compounds were obtained from the Streptomyces genus.
RESUMEN Objetivo: Investigar los metabolitos secundarios de los cultivos de Streptomyces sp CSDX076. Métodos: Los compuestos fueron aislados usando la cromatografía de columna y cromatografía líquida RP-18 de presión media. Sus estructuras fueron dilucidadas mediante métodos espectroscópicos de resonancia magnética nuclear unidimensional y bidimensional, combinados con experimentos de espectrometría de masa. Resultados: Cuatro compuestos de culturas de Streptomyces sp CSDX076 fueron aislados e identificados como benzoato de aurantiamida, deoxitriptoquivalina, ácido acético 2-acetil-3,5-dihidroxil-benzeno, y éster 2-acetil-3,5-dihidroxil-benzeno. Conclusión: Fue la primera vez que los cuatro compuestos aislados se obtuvieron del género Streptomyces.
Subject(s)
Streptomyces/chemistry , Benzoates/isolation & purification , Mass Spectrometry , Magnetic Resonance Spectroscopy , Chromatography, LiquidABSTRACT
Este estudo investiga as práticas de produção de conhecimento sobre a menopausa no Caism/Unicamp, centro de referência para políticas públicas em saúde da mulher. Foram realizadas observações de consultas ginecológicas, entrevistas com mulheres e médicos e observação de reuniões de apoio psicológico, buscando identificar os discursos que circulam no lugar e o processo de alistamento de diferentes atores para que os conhecimentos ali produzidos alcancem credibilidade e “viajem” além dos limites do hospital-escola, tornando-se “universais”. A análise baseia-se nos “estudos localistas”, alinhados aos estudos sociais de ciência e tecnologia.
This study investigates the practices involved in the production of knowledge about menopause at Caism, Unicamp, a reference center for public policies for women’s health. Gynecological appointments and psychological support meetings were observed, and women and doctors were interviewed in order to identify what discourse circulates there and how different actors are brought in to ensure that the knowledge produced attains credibility and “travels” beyond the boundaries of the teaching hospital to become “universal”. The analysis is based on localized studies aligned with social studies of science and technology.
Subject(s)
Animals , Male , Mice , /genetics , Major Histocompatibility Complex , Odorants , Benzoic Acid , Benzoates/isolation & purification , Benzoates/urine , Butyrates/isolation & purification , Butyrates/urine , Chromatography, Gas , Chromatography, Ion Exchange , Cresols/isolation & purification , Cresols/urine , Dimethyl Sulfoxide , Discrimination, Psychological , Maze Learning , Mice, Inbred Strains , Phenols/isolation & purification , Phenols/urine , Phenylacetates/isolation & purification , Phenylacetates/urine , Sulfones/isolation & purification , Sulfones/urine , UltrafiltrationABSTRACT
Se determinó el contenido de ácido benzoico en 698 muestras de alimentos de producción nacional y de importación, mediante el método de análisis normalizado para la red nacional de laboratorios de Higiene y Epidemiología. Se pudo determinar que los contenidos medios de ácido benzoico en todos los alimentos estudiados se encontraban por debajo del límite de tolerancia establecido y que los jugos refrigerados y refrescos carbonatados de frutas son las principales fuentes de ingestión de ácido benzoico dado su consumo y contenido del aditivo
Subject(s)
Food , Benzoates/isolation & purification , HygieneABSTRACT
Se determinó el contenido de ácido benzoico en 698 muestras de alimentos de producción nacional y de importación, mediante el método de análisis normalizado para la red nacional de laboratorios de Higiene y Epidemiología. Se pudo determinar que los contenidos medios de ácido benzoico en todos los alimentos estudiados se encontraban por debajo del límite de tolerancia establecido y que los jugos refrigerados y refrescos carbonatados de frutas son las principales fuentes de ingestión de ácido benzoico dado su consumo y contenido del aditivo