ABSTRACT
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
Subject(s)
Bone Matrix/growth & development , Gene Expression/radiation effects , Low-Level Light Therapy , Osseointegration/radiation effects , Osteoblasts/radiation effects , Alkaline Phosphatase/biosynthesis , Alkaline Phosphatase/genetics , Analysis of Variance , Bone Morphogenetic Protein 7/biosynthesis , Bone Morphogenetic Protein 7/genetics , Cells, Cultured/radiation effects , Collagen Type I/biosynthesis , Collagen Type I/genetics , Core Binding Factor Alpha 1 Subunit/biosynthesis , Core Binding Factor Alpha 1 Subunit/genetics , Humans , Integrin-Binding Sialoprotein/biosynthesis , Integrin-Binding Sialoprotein/genetics , Intercellular Adhesion Molecule-1/biosynthesis , Intercellular Adhesion Molecule-1/genetics , Lasers, Semiconductor/therapeutic use , Osteoblasts/metabolism , Osteocalcin/biosynthesis , Osteocalcin/genetics , Osteopontin/biosynthesis , Osteopontin/genetics , Osteoprotegerin/biosynthesis , Osteoprotegerin/genetics , RANK Ligand/biosynthesis , RANK Ligand/genetics , Statistics, Nonparametric , TitaniumABSTRACT
The aim of this study was to investigate the effects of low-level laser therapy (LLLT) by using gallium aluminum arsenide (GaAlAs) diode laser on human osteoblastic cells grown on titanium (Ti). Osteoblastic cells were obtained by enzymatic digestion of human alveolar bone and cultured on Ti discs for up to 17 days. Cells were exposed to LLLT at 3 J/cm2 (wavelength of 780 nm) at days 3 and 7 and non-irradiated cultures were used as control. LLLT treatment did not influence culture growth, ALP activity, and mineralized matrix formation. Analysis of cultures by epifluorescence microscopy revealed an area without cells in LLLT treated cultures, which was repopulated latter with proliferative and less differentiated cells. Gene expression of ALP, OC, BSP, and BMP-7 was higher in LLLT treated cultures, while Runx2, OPN, and OPG were lower. These results indicate that LLLT modulates cell responses in a complex way stimulating osteoblastic differentiation, which suggests possible benefits on implant osseointegration despite a transient deleterious effect immediately after laser irradiation.
Este estudo teve como objetivo investigar o efeito do laser diodo de gálio-alumÃnio-arsênio (GaAlAs) em células osteoblásticas humanas cultivadas sobre discos de Ti. Para tanto, células osteoblásticas foram obtidas por digestão enzimática de osso alveolar humano e cultivadas sobre discos de Ti por 17 dias. As células foram submetidas à irradiação no 3º e 7º dias na dose de 3 J/cm2 e comprimento de onda de 780 nm e células não irradiadas foram usadas como controle. A irradiação não alterou a proliferação celular, atividade de ALP e formação de matriz mineralizada. Microscopia por epifluorescência indicou que após 24 h da aplicação do laser, as culturas irradiadas apresentaram áreas sem células, que mais tarde foram repovoadas por células em fase de proliferação e menos diferenciadas. O laser aumentou a expressão gênica relativa da ALP, OC, BSP e BMP-7 e reduziu a de RUNX2, OPN e OPG. Os resultados indicam que a terapia com laser modula de forma complexa as respostas celulares, estimulando a diferenciação osteoblástica. Assim, é possÃvel sugerir possÃveis benefÃcios do laser na osseointegração de implantes de Ti apesar do efeito deletério à s células imediatamente após a irradiação.
Subject(s)
Humans , Bone Matrix/growth & development , Gene Expression/radiation effects , Low-Level Light Therapy , Osseointegration/radiation effects , Osteoblasts/radiation effects , Analysis of Variance , Alkaline Phosphatase/biosynthesis , Alkaline Phosphatase/genetics , /biosynthesis , /genetics , Cells, Cultured/radiation effects , Collagen Type I/biosynthesis , Collagen Type I/genetics , Core Binding Factor Alpha 1 Subunit/biosynthesis , Core Binding Factor Alpha 1 Subunit/genetics , Integrin-Binding Sialoprotein/biosynthesis , Integrin-Binding Sialoprotein/genetics , Intercellular Adhesion Molecule-1/biosynthesis , Intercellular Adhesion Molecule-1/genetics , Lasers, Semiconductor/therapeutic use , Osteoblasts/metabolism , Osteocalcin/biosynthesis , Osteocalcin/genetics , Osteopontin/biosynthesis , Osteopontin/genetics , Osteoprotegerin/biosynthesis , Osteoprotegerin/genetics , RANK Ligand/biosynthesis , RANK Ligand/genetics , Statistics, Nonparametric , TitaniumABSTRACT
El objetivo del estudio consitió en determinar los estadíos de maduración ósea según la clasificación de Helm y Björk. Para este fin, se tomaron radiografías de la mano como parte del procedimiento de diagnóstico ortodóncico al igual que las radiografíascefalométricas y panorámicas, en 80 pacientes niños que acudieron por tratamiento ortodóncico a la clínica de la Facultad de Odontología de la UNMSM, de los cuales 38 fueron mujeres y 42 varones. Las edades oscilaron entre los 7 y los 13 años de edad. Los resultados indicaron que las niñas alcanzaron el pico de crecimiento 2 años antes que los varones correspondientes al estadío MP3 cap. a los 10.29 años mientras quelos varones lo hicieron a los 12.12 años. Estos resultados demuestran que los niños peruanos maduran precozmente en relación a otras poblaciones.
The purpose of this study consisted in determining the stages of bony maturation according to the lassification of Helm and Björk, in 80 children patients who went for orthodontic treatment to the clinic of the Faculty of Odontology of UNMSM. Of the total of children, 38 were females and 42 were males. The ages oscillated between 7and 13 years of age. The results indicated that in the children, the stage MP3 cap that correspond to the growth tip found at the age of 10.29 years whereas in males the same phase occurred at the age of 12.12 years. These results demostrate that the Peruvian population matures early in relation to other population like the European.
Subject(s)
Humans , Child , Dental Care for Children , Dental Clinics , Age Determination by Teeth , Bone Matrix/growth & developmentABSTRACT
Os autores estudam experimentalmente o efeito do concentrado de plasma na estimulação óssea em camundongos. Foram utilizados dez camundongos isogênicos de linhagem gioto, onde após a coleta do sangue periférico de um camundongo, foi centrifugado este sangue e obtido um concentrado de plasma; foi utilizado o concentrado de plasma em falhas ósseas na região distal do fêmur, alternando-se os lados direito e esquerdo, sendo um lado com falha óssea isolada e o outro com falha óssea mais o concentrado de plasma e analisados quantitativamente e qualitativamente. Observou-se que o plasma não leva à estimulação da formação do calo ósseo, não ocorrendo aumento do processo inflamatório, e havendo apenas uma tendência a formar mais matriz óssea com o seu uso.