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1.
Elife ; 62017 09 19.
Article in English | MEDLINE | ID: mdl-28925352

ABSTRACT

The Bone Morphogenetic Protein (BMP) family reiteratively signals to direct disparate cellular fates throughout embryogenesis. In the developing dorsal spinal cord, multiple BMPs are required to specify sensory interneurons (INs). Previous studies suggested that the BMPs act as concentration-dependent morphogens to direct IN identity, analogous to the manner in which sonic hedgehog patterns the ventral spinal cord. However, it remains unresolved how multiple BMPs would cooperate to establish a unified morphogen gradient. Our studies support an alternative model: BMPs have signal-specific activities directing particular IN fates. Using chicken and mouse models, we show that the identity, not concentration, of the BMP ligand directs distinct dorsal identities. Individual BMPs promote progenitor patterning or neuronal differentiation by their activation of different type I BMP receptors and distinct modulations of the cell cycle. Together, this study shows that a 'mix and match' code of BMP signaling results in distinct classes of sensory INs.


Subject(s)
Bone Morphogenetic Proteins/metabolism , Cell Differentiation/drug effects , Interneurons/drug effects , Interneurons/physiology , Spinal Cord/embryology , Animals , Bone Morphogenetic Protein Receptors, Type I/agonists , Chickens , Mice , Models, Biological
2.
J Biol Chem ; 292(44): 17991-18005, 2017 11 03.
Article in English | MEDLINE | ID: mdl-28912273

ABSTRACT

The neuroligin (Nlg) family of neural cell adhesion molecules is thought to be required for synapse formation and development and has been linked to the development of autism spectrum disorders in humans. In Drosophila melanogaster, mutations in the neuroligin 1-3 genes have been reported to induce synapse developmental defects at neuromuscular junctions (NMJs), but the role of neuroligin 4 (dnlg4) in synapse development has not been determined. Here, we report that the Drosophila neuroligin 4 (DNlg4) is different from DNlg1-3 in that it presynaptically regulates NMJ synapse development. Loss of dnlg4 results in reduced growth of NMJs with fewer synaptic boutons. The morphological defects caused by dnlg4 mutant are associated with a corresponding decrease in synaptic transmission efficacy. All of these defects could only be rescued when DNlg4 was expressed in the presynapse of NMJs. To understand the basis of DNlg4 function, we looked for genetic interactions and found connections with the components of the bone morphogenetic protein (BMP) signaling pathway. Immunostaining and Western blot analyses demonstrated that the regulation of NMJ growth by DNlg4 was due to the positive modulation of BMP signaling by DNlg4. Specifically, BMP type I receptor thickvein (Tkv) abundance was reduced in dnlg4 mutants, and immunoprecipitation assays showed that DNlg4 and Tkv physically interacted in vivo Our study demonstrates that DNlg4 presynaptically regulates neuromuscular synaptic growth via the BMP signaling pathway by modulating Tkv.


Subject(s)
Cell Adhesion Molecules, Neuronal/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster , Nerve Tissue Proteins/metabolism , Neurogenesis , Neuromuscular Junction/metabolism , Presynaptic Terminals/metabolism , Protein Serine-Threonine Kinases/metabolism , Receptors, Cell Surface/metabolism , Animals , Animals, Genetically Modified , Bone Morphogenetic Protein Receptors, Type I/agonists , Bone Morphogenetic Protein Receptors, Type I/metabolism , Bone Morphogenetic Proteins/metabolism , Cell Adhesion Molecules, Neuronal/genetics , Drosophila Proteins/agonists , Drosophila Proteins/genetics , Gene Knockout Techniques , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Microscopy, Electron, Transmission , Microscopy, Fluorescence , Mutation , Nerve Tissue Proteins/genetics , Neuromuscular Junction/enzymology , Neuromuscular Junction/ultrastructure , Presynaptic Terminals/enzymology , Presynaptic Terminals/ultrastructure , Protein Multimerization , Recombinant Fusion Proteins/metabolism , Recombinant Proteins/metabolism , Signal Transduction , Synapses/enzymology , Synapses/metabolism , Synapses/ultrastructure , Synaptic Transmission
3.
J Orthop Res ; 33(2): 208-15, 2015 Feb.
Article in English | MEDLINE | ID: mdl-25331517

ABSTRACT

Bone Morphogenetic Protein 2 (BMP2) regulates bone integrity by driving both osteogenesis and osteoclastogenesis. However, BMP2 as a therapeutic has significant drawbacks. We have designed a novel peptide CK2.3 that blocks the interaction of Casein Kinase 2 (CK2) with Bone Morphogenetic Protein Receptor type Ia (BMPRIa), thereby activating BMP signaling pathways in the absence of ligand. Here, we show that CK2.3 induced mineralization in primary osteoblast cultures isolated from calvaria and bone marrow stromal cells (BMSCs) of 8 week old mice. Further, systemic tail vein injections of CK2.3 in 8 week old mice resulted in increased bone mineral density (BMD) and mineral apposition rate (MAR). In situ immunohistochemistry of the femur found that CK2.3 injection induced phosphorylation of extracellular signal-related kinase (ERK), but not Smad in osteocytes and osteoblasts, suggesting that CK2.3 signaling occurred through Smad independent pathway. Finally mice injected with CK2.3 exhibited decreased osteoclast differentiation and osteoclast activity. These data indicate that the novel mimetic peptide CK2.3 activated BMPRIa downstream signaling to enhance bone formation without the increase in osteoclast activity that accompanies BMP 2 stimulation.


Subject(s)
Bone Morphogenetic Protein Receptors, Type I/agonists , Casein Kinase II/antagonists & inhibitors , Osteogenesis/drug effects , Peptides/pharmacology , Animals , Animals, Newborn , Biomarkers/blood , Bone Density/drug effects , Cells, Cultured , Drug Evaluation, Preclinical , Extracellular Signal-Regulated MAP Kinases/metabolism , Female , Mesenchymal Stem Cells/drug effects , Mice, Inbred C57BL , Osteoclasts/drug effects , Skull/drug effects , Smad Proteins/metabolism
4.
Endocrinology ; 155(5): 1970-81, 2014 May.
Article in English | MEDLINE | ID: mdl-24601881

ABSTRACT

FSH is an essential regulator of mammalian reproduction. Its synthesis by pituitary gonadotrope cells is regulated by multiple endocrine and paracrine factors, including TGFß superfamily ligands, such as the activins and inhibins. Activins stimulate FSH synthesis via transcriptional regulation of its ß-subunit gene (Fshb). More recently, bone morphogenetic proteins (BMPs) were shown to stimulate murine Fshb transcription alone and in synergy with activins. BMP2 signals via its canonical type I receptor, BMPR1A (or activin receptor-like kinase 3 [ALK3]), and SMAD1 and SMAD5 to stimulate transcription of inhibitor of DNA binding proteins. Inhibitor of DNA binding proteins then potentiate the actions of activin-stimulated SMAD3 to regulate the Fshb gene in the gonadotrope-like LßT2 cell line. Here, we report the unexpected observation that BMP2 also stimulates the SMAD2/3 pathway in these cells and that it does so directly via ALK3. Indeed, this novel, noncanonical ALK3 activity is completely independent of ALK4, ALK5, and ALK7, the type I receptors most often associated with SMAD2/3 pathway activation. Induction of the SMAD2/3 pathway by ALK3 is dependent upon its own previous activation by associated type II receptors, which phosphorylate conserved serine and threonine residues in the ALK3 juxtamembrane glycine-serine-rich domain. ALK3 signaling via SMAD3 is necessary for the receptor to stimulate Fshb transcription, whereas its activation of the SMAD1/5/8 pathway alone is insufficient. These data challenge current dogma that ALK3 and other BMP type I receptors signal via SMAD1, SMAD5, and SMAD8 and not SMAD2 or SMAD3. Moreover, they suggest that BMPs and activins may use similar intracellular signaling mechanisms to activate the murine Fshb promoter in immortalized gonadotrope-like cells.


Subject(s)
Bone Morphogenetic Protein 2/metabolism , Bone Morphogenetic Protein Receptors, Type I/metabolism , Follicle Stimulating Hormone, beta Subunit/biosynthesis , Gonadotrophs/metabolism , Signal Transduction , Smad2 Protein/metabolism , Smad3 Protein/metabolism , Activins/antagonists & inhibitors , Activins/metabolism , Animals , Bone Morphogenetic Protein 2/agonists , Bone Morphogenetic Protein 2/antagonists & inhibitors , Bone Morphogenetic Protein 2/genetics , Bone Morphogenetic Protein Receptors, Type I/agonists , Bone Morphogenetic Protein Receptors, Type I/antagonists & inhibitors , Bone Morphogenetic Protein Receptors, Type I/genetics , Cell Line , Follicle Stimulating Hormone, beta Subunit/genetics , Follicle Stimulating Hormone, beta Subunit/metabolism , Gene Silencing , Genes, Reporter , Humans , Mice , Phosphorylation , Protein Processing, Post-Translational , RNA, Small Interfering , Recombinant Proteins/chemistry , Recombinant Proteins/metabolism , Smad2 Protein/antagonists & inhibitors , Smad2 Protein/genetics , Smad3 Protein/antagonists & inhibitors , Smad3 Protein/genetics , Transcription, Genetic
5.
Cell Stem Cell ; 7(1): 78-89, 2010 Jul 02.
Article in English | MEDLINE | ID: mdl-20621052

ABSTRACT

Neural stem cells (NSCs) in the adult hippocampus divide infrequently, and the molecules that modulate their quiescence are largely unknown. Here, we show that bone morphogenetic protein (BMP) signaling is active in hippocampal NSCs, downstream of BMPR-IA. BMPs reversibly diminish proliferation of cultured NSCs while maintaining their undifferentiated state. In vivo, acute blockade of BMP signaling in the hippocampus by intracerebral infusion of Noggin first recruits quiescent NSCs into the cycle and increases neurogenesis; subsequently, it leads to decreased stem cell division and depletion of precursors and newborn neurons. Consistently, selective ablation of Bmpr1a in hippocampal NSCs, or inactivation of BMP canonical signaling in conditional Smad4 knockout mice, transiently enhances proliferation but later leads to a reduced number of precursors, thereby limiting neuronal birth. BMPs are therefore required to balance NSC quiescence/proliferation and to prevent loss of the stem cell activity that supports continuous neurogenesis in the mature hippocampus.


Subject(s)
Bone Morphogenetic Protein Receptors, Type I/metabolism , Hippocampus/cytology , Neurons/cytology , Neurons/metabolism , Stem Cells/cytology , Stem Cells/metabolism , Animals , Bone Morphogenetic Protein Receptors, Type I/agonists , Bone Morphogenetic Protein Receptors, Type I/genetics , Carrier Proteins/pharmacology , Cell Cycle/genetics , Cell Cycle/physiology , Cell Line , Cells, Cultured , Flow Cytometry , Genetic Vectors , Humans , Lentivirus , Mice , Models, Biological , Neurons/drug effects , Rats , Rats, Inbred F344 , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction/drug effects , Signal Transduction/genetics , Smad4 Protein/genetics , Smad4 Protein/metabolism , Stem Cells/drug effects
6.
Steroids ; 74(13-14): 1066-72, 2009.
Article in English | MEDLINE | ID: mdl-19723531

ABSTRACT

We recently identified that cholesterol myristate in traditional Chinese medicine (TCM) is the active compound that increases proliferation of mesenchymal stem cell (MSCs). The present study is further to determine what signal pathway involves in effect of cholesterol myristate. Reverse transcription-PCR, Western blot and ELISA analysis show that cholesterol myristate increases the release of bone morphogenetic protein 4 (BMP4) from MSCs and the expression in the intracellular levels of BMP4 in a time- and dose dependent manner. However, structurally related steroids such as cholesterol and cholesten presented in TCM, both lack of the myristate, did not affect the secretion and expression of BMP4 on MSCs. These finds suggest that myristate is essential for the effects of cholesterol myristate. Furthermore, cholesterol myristate significantly increase BMPRIB levels of MSCs and the number of BMPRIB positive cells in a time- and dose dependent manner, but not BMPR IA or BMPR II. Our results indicate that action of cholesterol myristate may activate the BMP4-BMPRIB autocrine. Moreover, a blocking antibody against BMP4 or the BMP4 antagonist, noggin, partially reduced the effects of cholesterol myristate on MSCs proliferation. Thus, this study is to provide evidence that autocrine BMP4 signaling involves effect of cholesterol myristate on MSCs proliferation.


Subject(s)
Autocrine Communication/drug effects , Bone Morphogenetic Protein 4/metabolism , Bone Morphogenetic Protein Receptors, Type I/metabolism , Cell Proliferation/drug effects , Cholesterol Esters/pharmacology , Mesenchymal Stem Cells/drug effects , Animals , Antibodies, Neutralizing/immunology , Autocrine Communication/physiology , Bone Morphogenetic Protein 4/agonists , Bone Morphogenetic Protein 4/antagonists & inhibitors , Bone Morphogenetic Protein Receptors, Type I/agonists , Carrier Proteins/pharmacology , Mesenchymal Stem Cells/metabolism , Mesenchymal Stem Cells/physiology , Rats , Rats, Sprague-Dawley , Signal Transduction/drug effects
7.
Biochem Biophys Res Commun ; 377(3): 905-9, 2008 Dec 19.
Article in English | MEDLINE | ID: mdl-18952055

ABSTRACT

Fibrodysplasia ossificans progressiva (FOP) is a rare autosomal dominant congenital disorder characterized by progressive heterotopic bone formation in muscle tissues. A common mutation among FOP patients has been identified in ALK2, ALK2(R206H), which encodes a constitutively active bone morphogenetic protein (BMP) receptor. Recently, a unique mutation of ALK2, ALK2(G356D), was identified to be a novel mutation in a Japanese FOP patient who had unique clinical features. Over-expression of ALK2(G356D) induced phosphorylation of Smad1/5/8 and activated Id1-luc and alkaline phosphatase activity in myoblasts. However, the over-expression failed to activate phosphorylation of p38, ERK1/2, and CAGA-luc activity. These ALK2(G356D) activities were weaker than those of ALK2(R206H), and they were suppressed by a specific inhibitor of the BMP-regulated Smad pathway. These findings suggest that ALK2(G356D) induces heterotopic bone formation via activation of a BMP-regulated Smad pathway. The quantitative difference between ALK2(G356D) and ALK2(R206H) activities may have caused the phenotypic differences in these patients.


Subject(s)
Activin Receptors, Type I/genetics , Activin Receptors, Type I/metabolism , Bone Morphogenetic Protein Receptors, Type I/metabolism , Mutation , Myositis Ossificans/enzymology , Myositis Ossificans/genetics , Activin Receptors, Type I/antagonists & inhibitors , Amino Acid Substitution , Animals , Aspartic Acid/genetics , Aspartic Acid/metabolism , Bone Morphogenetic Protein Receptors, Type I/agonists , Bone Morphogenetic Protein Receptors, Type I/antagonists & inhibitors , Cell Differentiation , Glycine/genetics , Glycine/metabolism , Humans , Ligands , Mice , Muscle Development/drug effects , Muscle Development/genetics , Myoblasts/drug effects , Myoblasts/metabolism , Osteoblasts/drug effects , Pyrazoles/pharmacology , Pyrimidines/pharmacology , Signal Transduction , Smad Proteins/metabolism
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