Strategies for producing probiotic biomass and postbiotics from Akkermansia muciniphila in submerged cultivations incorporating prebiotic sources.

This research propounds an innovative technology focused on sustainability to increase the biomass yield of Akkermansia muciniphila, the next-generation probiotic, using prebiotic sources to replace or reduce animal mucin levels. A series of experimental design approaches were developed aiming to optimize the growth of Akkermansiamuciniphila by incorporating extracts of green leafy vegetables and edible mushroom into the cultivation media. Experiments using kale extract (KE), Brassica oleracea L., associated with lyophilized mushroom extract (LME) of Pleurotus ostreatus were the most promising, highlighting the assays with 0.376% KE and 0.423% LME or 1.05% KE and 0.5% LME, in which 3.5 × 1010 CFU (Colony Forming Units) mL- 1 was achieved - higher than in experiments in optimized synthetic media. Such results enhance the potential of using KE and LME not only as mucin substitutes, but also as a source to increase Akkermansia muciniphila biomass yields and release short-chain fatty acids. The work is relevant to the food and pharmaceutical industries in the preparation of the probiotic ingredient.

In vitro safety profile and phyto-ecotoxicity assessment of the eco-friendly calcium oxide nanoparticles.

The present study aims to evaluate the toxicity of the green calcium oxide nanoparticles (CaO-NPs) from golden linseed extract (Linum usitatissimum L.) by phytotoxicity in seeds (Daucus carota, Beet shankar, Lactuca sativa and Brassica oleracea), in vitro safety profile and soil toxicity for CaO-NPs solutions from 12.5 to 100 µg mL-1. Ecotoxicity analysis of the soil was conducted using XRD diffractograms, which revealed characteristic peaks of the nanoparticles at 37.35° (12.5, 25, 50, and 100 µg mL-1), as well as a peak at 67.34° (25 and 100 µg mL-1). Additionally, the in vitro safety assessment indicated favorable cell specification and regulation within the first 24 h, demonstrating reductions of 15.9 ± 0.2%, 17.9 ± 0.2%, 17.6 ± 0.2%, and 32.9 ± 0.2% to 12.5, 25, 50, and 100 µg mL-1, respectively. The dsDNA assay revealed initial protection and controlled release within the cells for 48 h. However, after 72 h, there was an increase of 20 ± 0.2%, 16 ± 0.2%, 32 ± 0.2%, and 43 ± 0.2% to 12.5, 25, and 50 µg mL-1. The analysis of ROS generation demonstrated a reduction of 40 ± 0.2%, 33 ± 0.2%, 20 ± 0.2%, and 9 ± 0.2% to 12.5, 25, 50, and 100 µg mL-1, respectively, within 72 h. When compared to the negative control (NC), there was an increase of 50 ± 0.2%, 56 ± 0.2%, 77 ± 0.2%, and 92 ± 0.2% at the same concentrations, suggesting that the nanoparticles generated free radicals, leading to cellular inflammation. This was attributed to the positive surface charge of the nanoparticles, resulting in reduced interaction with the cell membrane and the subsequent release of hydroxyl (•OH), which caused inflammatory processes in the cells. Therefore, CaO-NPs exhibited a low phytotoxicity and high cytocompatibility, while also promoting plant germination and growth.

Sample size affects the precision of the analysis of variance in experiments with cauliflower seedlings / O tamanho de amostra afeta a precisão da análise de variância em experimentos com mudas de couve-flor

This study verified whether sample size would affect the precision of the analysis of variance in experiments with cauliflower seedlings. An experiment was carried out where the number of leaves and shoot, root and total length were measured. For each variable, resamplings with repositions were performed in sample scenarios of 1, 2, …, 100 seedlings per experimental unit, and the sample size was defined for the variance components through Schumacher models and maximum curvature points. The mean squares of the analysis of variance suffer direct interference from the number of sampled seedlings. The sampling of 16 seedlings per experimental unit is enough to estimate the analysis of variance reliably, promoting satisfactory precision gains compared to the sampling of only one seedling per experimental unit.

Este estudo verificou se o tamanho de amostra afetaria a precisão da análise de variância em experimentos com mudas de couve-flor. Um experimento foi conduzido onde o número de folhas, comprimento de parte aérea, raiz e total foram mensurados. Para cada variável, reamostragens com reposição foram realizadas em cenários amostrais de 1, 2, ..., 100 mudas por unidade experimental e o tamanho de amostra foi definido para os componentes de variância por meio de modelos de Schumacher e pontos de máxima curvatura. Os quadrados médios da análise de variância sofrem interferência direta do número de mudas amostradas. A amostragem de 16 mudas por unidade experimental é suficiente para estimar a análise de variância de forma confiável, promovendo satisfatórios ganhos de precisão ao comparar-se com a amostragem de apenas uma muda por unidade experimental.

Effect of foliar application of bio-stimulants on growth, yield and nutritional quality of broccoli / Efeito da aplicação foliar de bioestimulantes no crescimento, produtividade e qualidade nutricional de brócolis

Broccoli is one of important cole crop grown all over the world due to its unique nutritional profile consumed fresh as well as processed. It contains a wide range of nutrients, vitamins, minerals and specific anti-cancer compounds such as glucosinolates. Broccoli cultivation in Pakistan is increasing rapidly, however, till now there is no standardized cropping technology for broccoli cultivation under local climate. Considering research gap (lack of suitable varieties, poor growth, and unavailability of optimized crop technology), trial was conducted at Vegetable research area, Institute of Horticultural Sciences, University of Agriculture, Faisalabad to evaluate the impact of bio-stimulants on different broccoli cultivars under local climatic conditions. The set of experimental treatments was laid out in Randomized Complete Block Design (RCBD) with three replications. Pre-harvest application of Isabion and Seaweed extract significantly enhanced the plant height (11%), dry weight (4%), leaf area (7%), and yield plant-1 (5%). Moreover, Isabion and seaweed extract application led to the increase in antioxidant enzymes i.e., superoxide dismutase (18%), peroxidase (38%) and catalase (12%). In crux, the foliar application of bio-stimulants (Isabion and seaweed extract) on broccoli enhanced the growth, yield, and contents of antioxidant enzymes.

O brócolis é uma das importantes culturas de repolho cultivadas em todo o mundo devido ao seu perfil nutricional único consumido fresco e processado. Contém uma ampla gama de nutrientes, vitaminas, minerais e compostos anticancerígenos específicos, como glucosinolatos. O cultivo de brócolis no Paquistão está aumentando rapidamente, no entanto, até agora não há tecnologia de cultivo padronizada para o seu cultivo sob o clima local. Considerando a lacuna de pesquisa, como a falta de variedades adequadas, o baixo crescimento e a indisponibilidade de tecnologia de cultivo otimizada, o teste foi realizado na área de pesquisa de vegetais, no Instituto de Ciências Hortícolas na Universidade de Agricultura, em Faisalabad, Paquistão, para avaliar o impacto de bioestimulantes em diferentes cultivares de brócolis nas condições climáticas locais. O conjunto de tratamentos experimentais foi disposto em Randomized Complete Block Design (RCBD) com três repetições. A aplicação pré-colheita de Isabion e extrato de algas marinhas aumentou significativamente a altura da planta (11%), peso seco (4%), área foliar (7%) e rendimento planta (5%). Além disso, a aplicação de Isabion e extrato de algas marinhas levou ao aumento das enzimas antioxidantes, ou seja, superóxido dismutase (18%), peroxidase (38%) e catalase (12%). No crux, a aplicação foliar de bioestimulantes (Isabion e extrato de algas marinhas) melhorou o crescimento, rendimento e conteúdo de enzimas antioxidantes do vegetal

Replacing 30 % chemical fertilizer with organic fertilizer increases the fertilizer efficiency, yield and quality of cabbage in intensive open-field production / Substituindo 30% de fertilizante químico por orgânico aumenta a eficiência, o rendimento e a qualidade do repolho na produção intensiva em campo aberto

The combined application of chemical fertilizer and organic fertilizer is an effective way to improve soil fertility, crop yield and quality. In this study, a field experiment was conducted to evaluate the yield, quality and fertilizer use efficiency of cabbage (Brassica oleracea var. capitata L.) grown under different ratios of organic fertilizer replacing chemical fertilizer at a rate of 225 kg N ha-l in 2019 and 2020. The fertilizer treatments included N0 (non nitrogen fertilizer), CK (only compound fertilizer was applied, N:P:K=15.0 %:6.5 %:12.4 %), T1, T2 and T3 (organic fertilizer replaced 15 %, 30 % and 45 % chemical fertilizer, respectively), and T4 (total nutrient input was reduced by 10 % under T2). Results showed that T2 had the highest marketable yield and fertilizer use efficiency, as well as the best quality. The marketable yield, vitamin C content, soluble sugar content, REN (apparent uptake efficiency of fertilizer N) and AEN (agronomic N use efficiency) of T2 increased by 32.2 %, 14,9 %, 5.5 %, 97. % and 55.6 %, respectively, in contrast, the crude fibers decreased by 34.0 %, compared with CK in the two years. In addition, T4 guaranteed the yield and moderately improved the quality of cabbage, compared with CK. Therefore, we can alternatively apply fertilization according to the purpose of capturing the highest yield or properly declining fertilizer to sustain soil texture and productivity.

A aplicação combinada de fertilizante químico e o orgânico é uma forma eficaz de melhorar a fertilidade do solo, o rendimento e a qualidade da colheita. Neste estudo, um experimento de campo foi conduzido para avaliar o rendimento, a qualidade e a eficiência do uso de fertilizantes em repolho (Brassica oleracea var. Capitata L.) cultivado sob diferentes proporções de fertilizante orgânico em substituição ao químico a uma taxa de 225 kg N ha-l em 2019 e 2020. Os tratamentos de fertilizantes incluíram N0 (fertilizante sem nitrogênio), CK (apenas fertilizante composto foi aplicado, N: P: K = 15,0%: 6,5%: 12,4%), T1, T2 e T3 (fertilizante orgânico substituído 15%, 30% e 45% de fertilizante químico, respectivamente) e T4 (a entrada total de nutrientes foi reduzida em 10% em T2). Os resultados mostraram que o T2 apresentou o maior rendimento comercial e eficiência no uso de fertilizantes, bem como a melhor qualidade. O rendimento comercializável, teor de vitamina C, teor de açúcar solúvel, REN (eficiência de absorção aparente de fertilizante N) e AEN (eficiência de uso de N agronômico) de T2 aumentaram 32,2%, 14,9%, 5,5%, 97% e 55,6%, respectivamente, em contraste, as fibras brutas diminuíram 34,0%, em comparação com a CK nos dois anos. Além disso, o T4 garantiu o rendimento e melhorou moderadamente a qualidade do repolho, em comparação com o CK. Portanto, podemos alternativamente aplicar fertilização de acordo com o objetivo de obter o maior rendimento ou fertilizante em declínio adequado para sustentar a textura e a produtividade do solo.

Impact of Diamondback Moth Density and Infestation Timing on Broccoli Yield.

The diamondback moth (DBM), Plutella xylostella (L.) (Lep.: Plutellidae), is an important pest of broccoli (Brassica oleracea L. var. italica Plenck). Few studies have focused on the real DBM impact on broccoli yield. We performed greenhouse studies to assess the effect of DBM densities and infestation timing (at pre-heading, heading, or during the entire cycle) on broccoli head weight. Polynomial trend analysis revealed a downward linear response of head weight to DBM densities, indicating that broccoli is susceptible to DBM attack. As for the infestation timing, infestations during the pre-heading stage significantly impacted head weight, whereas the same did not occur for infestations during the heading stage. DBM density did not affect plant height or total leaves but was upward-related to plant defoliation, and head weight correlated negatively with plant defoliation. These findings indicate that pre-heading is the determining stage for head weight loss of plants under DBM attack. In order to prevent quantitative losses, efforts for DBM management should be focused on this particular stage.

Development of cauliflower seedlings due to the increase of silicon in substrate / Desenvolvimento de mudas de couve-flor em função do incremento de silício no substrato

The production of seedlings is an important step in the establishment of the crop, with repercussions on yield, whose application of Si can improve phytosanitary and performance characteristics. The objective of the study was to analyze the increase of silicon in substrate on the development of seedlings of Brassica oleracea var. botrytis. The experiment carried out in randomized blocks, with five amounts of silicon (0, 25, 50, 75 and 100 g kg-1of substrate) and four replications. Silicon oxide (98% SiO2) used, mixed with the substrate with subsequent filling of the polyethylene trays (128 cells) and seeding. The trays kept in a greenhouse. At 30 days after sowing, the height of seedlings, number of leaves, fresh weight of leaves and stem, dry weight of leaves, stem and root evaluated. The data subjected to analysis of variance by the F test and regression analysis. Of the variables analyzed, only the dry root mass did not show a significant difference. Quadratic models can represent the variation in components. Seedling height increased with the increase of silicon up to the dose of 75 g kg-1. Other variables showed increases up to a dose of 50 g kg-1. The increase of silicon in the substrate was efficient in the development of seedlings of Brassica oleraceavar. botrytisin an amount of up to 50 g kg-1of substrate.(AU)

A produção de mudas é etapa importante do estabelecimento da cultura, com reflexos no rendimento, cuja aplicação de Si pode melhorar características fitossanitárias e de desempenho. O objetivo do estudo foi analisar o incremento de silício em substrato sobre o desenvolvimento de mudas de Brassica oleracea var. Botrytis . O experimento foi desenvolvido em blocos casualizados, com cinco quantidades de silício (0, 25, 50, 75 e 100 g kg-1de substrato) e 4 repetições. Foi utilizado óxido de silício (98%SiO2), sendo misturado ao substrato com posterior preenchimento das bandejas de polietileno (128 células) e semeadura. As bandejas foram mantidas em casa de vegetação. Aos 30 dias após a semeadura foi avaliado a altura das mudas, número de folhas, massa fresca das folhas e caule, massa seca das folhas, caule e raiz. Os dados foram submetidos a análise de variância pelo teste F e análise de regressão. Das variáveis analisadas, apenas a massa seca de raízes não apresentou diferença significativa. Modelos quadráticos podem representar a variação dos componentes. A altura de mudas foi crescente com o incremento de silício até a dose de 75 g kg-1. Demais variáveis apresentaram acréscimos até dose de 50 g kg-1. O incremento de silício no substrato foi eficiente no desenvolvimento de mudas de Brassica oleraceavar. botrytisem quantidade até 50 g kg-1de substrato.(AU)

Development of cauliflower seedlings due to the increase of silicon in substrate / Desenvolvimento de mudas de couve-flor em função do incremento de silício no substrato

The production of seedlings is an important step in the establishment of the crop, with repercussions on yield, whose application of Si can improve phytosanitary and performance characteristics. The objective of the study was to analyze the increase of silicon in substrate on the development of seedlings of Brassica oleracea var. botrytis. The experiment carried out in randomized blocks, with five amounts of silicon (0, 25, 50, 75 and 100 g kg-1of substrate) and four replications. Silicon oxide (98% SiO2) used, mixed with the substrate with subsequent filling of the polyethylene trays (128 cells) and seeding. The trays kept in a greenhouse. At 30 days after sowing, the height of seedlings, number of leaves, fresh weight of leaves and stem, dry weight of leaves, stem and root evaluated. The data subjected to analysis of variance by the F test and regression analysis. Of the variables analyzed, only the dry root mass did not show a significant difference. Quadratic models can represent the variation in components. Seedling height increased with the increase of silicon up to the dose of 75 g kg-1. Other variables showed increases up to a dose of 50 g kg-1. The increase of silicon in the substrate was efficient in the development of seedlings of Brassica oleraceavar. botrytisin an amount of up to 50 g kg-1of substrate.

A produção de mudas é etapa importante do estabelecimento da cultura, com reflexos no rendimento, cuja aplicação de Si pode melhorar características fitossanitárias e de desempenho. O objetivo do estudo foi analisar o incremento de silício em substrato sobre o desenvolvimento de mudas de Brassica oleracea var. Botrytis . O experimento foi desenvolvido em blocos casualizados, com cinco quantidades de silício (0, 25, 50, 75 e 100 g kg-1de substrato) e 4 repetições. Foi utilizado óxido de silício (98%SiO2), sendo misturado ao substrato com posterior preenchimento das bandejas de polietileno (128 células) e semeadura. As bandejas foram mantidas em casa de vegetação. Aos 30 dias após a semeadura foi avaliado a altura das mudas, número de folhas, massa fresca das folhas e caule, massa seca das folhas, caule e raiz. Os dados foram submetidos a análise de variância pelo teste F e análise de regressão. Das variáveis analisadas, apenas a massa seca de raízes não apresentou diferença significativa. Modelos quadráticos podem representar a variação dos componentes. A altura de mudas foi crescente com o incremento de silício até a dose de 75 g kg-1. Demais variáveis apresentaram acréscimos até dose de 50 g kg-1. O incremento de silício no substrato foi eficiente no desenvolvimento de mudas de Brassica oleraceavar. botrytisem quantidade até 50 g kg-1de substrato.

Silicon attenuates calcium deficiency by increasing ascorbic acid content, growth and quality of cabbage leaves.

Calcium (Ca) deficiency in cabbage plants induces oxidative damage, hampering growth and decreasing quality, however, it is hypothesized that silicon (Si) added to the nutrient solution may alleviate crop losses. Therefore, this study aims at evaluating whether silicon supplied in the nutrient solution reduces, in fact, the calcium deficiency effects on cabbage plants. In a greenhouse, cabbage plants were grown using nutrient solutions with Ca sufficiency and Ca deficiency (5 mM) without and with added silicon (2.5 mM), arranged as a 2 × 2 factorial in randomized blocks, with five replications. At 91 days after transplanting, the plants were harvested for biological evaluations. In the treatment without added Si, Ca deficiency promoted oxidative stress, low antioxidant content, decreased dry matter, and lower quality leaf. On the other hand, added Si attenuated Ca deficiency in cabbage by decreasing cell extravasation while increasing both ascorbic acid content and fresh and dry matter, providing firmer leaves due to diminished leaf water loss after harvesting. We highlighted the agronomic importance of Si added to the nutrient solution, especially in crops at risk of Ca deficiency.

Expression of BoNOL and BoHCAR genes during postharvest senescence of broccoli heads.

BACKGROUND: Chlorophyll is the most abundant pigment on Earth, essential for the capture of light energy during photosynthesis. During senescence, chlorophyll degradation is highly regulated in order to diminish toxicity of the free chlorophyll molecule due to its photoactivity. The first step in the chlorophyll degradation pathway is the conversion of chlorophyll b to chlorophyll a by means of two consecutive reactions catalyzed by enzymes coded by NYC1 (NON-YELLOW COLORING 1), NOL (NYC1-LIKE) and HCAR. RESULTS: In this work, we studied the expression of NOL and HCAR genes during postharvest senescence of broccoli. We found that the expression of BoNOL increase during the first days of storage and then decrease. In the case of BoHCAR, its expression is maintained during the first days and then it also diminishes. Additionally, the effect of different postharvest treatments on the expression of these genes was also analyzed. It was observed that the expression of BoNOL is lower in the treatments performed with 1-methylcyclopropene (1-MCP), 6-benzylaminopurine (6-BAP) and modified atmospheres, while BoHCAR expression showed an increase in these same treatments, and a decrease in the treatment with ethylene. There were no variations in the expression of both genes in heat treatment, UV-C treatment and visible light treatment. CONCLUSIONS: These results suggest that both BoHCAR and BoNOL show a lower regulation of their expression than other genes involved in chlorophyll degradation during senescence. © 2020 Society of Chemical Industry.

Determination of evapotranspiration and crop coefficient for cauliflower at Paranánorthwest region / Determinação da evapotranspiração e coeficiente da cultura da couve-flor na região noroeste do Paraná

Evapotranspirationand the estimation of crop coefficient (Kc) are essential information for irrigation management, contributing to increase the water use efficiency and optimizing resources, mainly using local data. This study aimed to determine the water consumption and the crop coefficient of cauliflower (Barcelona hybrid) in the northwestern region of Paraná. The experiment was conducted in a greenhouse at the Technical Irrigation Centerbelonging to the State University of Maringá, in Maringá -PR. The crop evapotranspiration was determined using constant water table lysimeters, and the reference evapotranspiration was estimated by the Penman-Monteith method (FAO), using climatological data collected in an automatic meteorological station. The cultivation was carried out from April to July. The total water consumption by the cauliflower was 215.11 mm, a higher value than that available in the FAO recommendations. The crop coefficient (Kc) recommended values for local conditions are 0.74 for the initial stage, 1.86 for the intermediate stage, and 1.50 for the final stage.(AU)

A evapotranspiraçãoe a estimativa dos coeficientes da cultura (Kc) são informações fundamentais para o manejo da irrigação, contribuindo para o aumento da eficiência no uso da água e otimização dos recursos, principalmente a utilizar dados locais. O trabalho teve como objetivo determinar o consumo hídrico e o coeficiente da cultura da couve-flor (híbrido Barcelona) na região noroeste do Paraná. O experimento foi conduzido em casa de vegetação no Centro Técnico de Irrigação pertencente à Universidade Estadual de Maringá, em Maringá -PR. A evapotranspiração da cultura foi determinada utilizando lisímetros de lençol freático constante, e a evapotranspiração de referência foi estimada pelo método de Penman-Monteith (FAO), a partir de informações climatológicas coletadas em estação meteorológica automática. O cultivo foi realizado no período de abril a julho. O consumo total de água pela cultura da couve-flor foi de 215,11 mm, sendo um valor maior do que o disponível nas recomendações da FAO. Os valores recomendados de coeficiente da cultura (Kc) para as condições de cultivo são de 0,74 para o estádio inicial, 1,86 para o estádio intermediário e 1,50 para o estádio final.(AU)

Determination of evapotranspiration and crop coefficient for cauliflower at Paranánorthwest region / Determinação da evapotranspiração e coeficiente da cultura da couve-flor na região noroeste do Paraná

Evapotranspirationand the estimation of crop coefficient (Kc) are essential information for irrigation management, contributing to increase the water use efficiency and optimizing resources, mainly using local data. This study aimed to determine the water consumption and the crop coefficient of cauliflower (Barcelona hybrid) in the northwestern region of Paraná. The experiment was conducted in a greenhouse at the Technical Irrigation Centerbelonging to the State University of Maringá, in Maringá -PR. The crop evapotranspiration was determined using constant water table lysimeters, and the reference evapotranspiration was estimated by the Penman-Monteith method (FAO), using climatological data collected in an automatic meteorological station. The cultivation was carried out from April to July. The total water consumption by the cauliflower was 215.11 mm, a higher value than that available in the FAO recommendations. The crop coefficient (Kc) recommended values for local conditions are 0.74 for the initial stage, 1.86 for the intermediate stage, and 1.50 for the final stage.

A evapotranspiraçãoe a estimativa dos coeficientes da cultura (Kc) são informações fundamentais para o manejo da irrigação, contribuindo para o aumento da eficiência no uso da água e otimização dos recursos, principalmente a utilizar dados locais. O trabalho teve como objetivo determinar o consumo hídrico e o coeficiente da cultura da couve-flor (híbrido Barcelona) na região noroeste do Paraná. O experimento foi conduzido em casa de vegetação no Centro Técnico de Irrigação pertencente à Universidade Estadual de Maringá, em Maringá -PR. A evapotranspiração da cultura foi determinada utilizando lisímetros de lençol freático constante, e a evapotranspiração de referência foi estimada pelo método de Penman-Monteith (FAO), a partir de informações climatológicas coletadas em estação meteorológica automática. O cultivo foi realizado no período de abril a julho. O consumo total de água pela cultura da couve-flor foi de 215,11 mm, sendo um valor maior do que o disponível nas recomendações da FAO. Os valores recomendados de coeficiente da cultura (Kc) para as condições de cultivo são de 0,74 para o estádio inicial, 1,86 para o estádio intermediário e 1,50 para o estádio final.

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications.

BACKGROUND: Biosurfactants are surface-active agents produced by microorganisms that have higher efficiency and stability, lower toxicity and higher biocompatibility and biodegradability than chemical surfactants. Despite its properties and potential application in a wide range of environmental and industrial processes, biosurfactants are still not cost-competitive when compared to their synthetic counterparts. Cost effective technologies and renewable raw substrates as agro-industrial and regional waste from northeast of Brazil as cassava flour wastewater, supplemented with lactose and corn oil are mainly the chemically media for growing microorganism and in turn the production of the biosurfactant of quality. This study aimed to obtained biosurfactant by Serratia marcescens UCP 1549 containing cassava flour wastewater (CWW), by application of a full-factorial design, as sustainable practices in puts the production process in promising formulation medium. The characterization of the biomolecule was carried out, as well as the determination of its stability and toxicity for cabbage seeds. In addition, its ability to stimulate seed germination for agriculture application and oil spill bioremediation were investigated. RESULTS: Serratia marcescens showed higher reduction of surface tension (25.92 mN/m) in the new medium containing 0.2% lactose, 6% cassava flour wastewater and 5% corn waste oil, after 72 h of fermentation at 28 °C and 150 rpm. The substrate cassava flour wastewater showed a promising source of nutrients for biosurfactant production. The isolate biosurfactant exhibited a CMC of 1.5% (w/v) and showed an anionic and polymeric structure, confirmed by infrared spectra. The biomolecule demonstrated high stability under different temperatures, salinity and pH values and non-toxicity against to cabbage seeds. Thus, exploring biosurfactant their potential role in seeds germinations and the promotion and agricultural applications was investigated. In addition, the effectiveness of biosurfactant for removal burned motor oil adsorbed in sand was verified. CONCLUSIONS: The use of medium containing CWW not only reduces the cost of process of biosurfactant production, but also the environmental pollution due to the inappropriate disposal of this residue. This fact, added to the high stability and non-toxicity of the biosurfactant produced by S. marcescens UCP 1549, confirms its high environmental compatibility, make it a sustainable biocompound that can be replace chemical surfactants in diverse industries. In addition, the effectiveness of biosurfactant for stimulate seed germination and removing burned motor oil from sand, suggests its suitability for agriculture and bioremediation applications.

Collard green yield and nutritional quality with mineral and organic fertilization / Produção e qualidade nutricional de couve-de-folha com adubação mineral e orgânica

A study was conducted to investigate the effect of organic and mineral fertilization on collard green yield, and to assess the macro and micronutrient and heavy metal concentrations of its leaves to assess the potential hazards of manure use. Collard plants (Brassica oleracea var. acephala, cv. couve-manteiga) were grown in 4-L pots filled with a clay soil under greenhouse conditions. The experimental design used was completely randomized design and the treatments in a 3 × 4 factorial arrangement: three N sources (pig slurry, poultry manure, and urea) and four N rates (0, 100, 200 and 300 mg kg-1), with four replicates. Collard greens leaves were harvested 70 days after seedling plantings. The manure application (pig slurry or poultry manure) resulted in collard yield equivalent to the application of mineral fertilizer. However, the pig slurry application resulted in an increase of leaf Fe, Mn, Zn, Cd, Cr, and Pb concentrations. Nitrogen application rates up to 300 mg N kg-1 result in the higher yield and nutritional quality of collard leaves. The application of high rates of manure, especially pig slurry, results in the increase of heavy metal (Cd, Cr, and Pb) concentrations. However, the maximum concentrations of 0.042 mg kg-1 Cd, 0.034 mg kg-1 Cr and 0.030 mg kg-1 Pb are well lower than maximum daily intake limit established by the Brazilian legislation, indicating that the consumption of collards from organic production systems with the manure application can be considered safe and without risk to human health.(AU)

Foi realizado um estudo para avaliar o efeito da aplicação de esterco e fertilizante mineral na produção e na qualidade nutricional de couve (Brassica oleracea var. acephala, cv. couve-manteiga). As plantas de couve foram cultivadas em vasos de 4 L preenchidos com um solo argiloso em condições de casa-de-vegetação. O delineamento experimental utilizado foi o inteiramente casualizado e os tratamentos em um arranjo fatorial 3 × 4: três fontes de N (dejetos de suínos, esterco de aves e ureia) e quatro doses de N (0, 100, 200 e 300 mg kg-1), com quatro repetições. As folhas de couve foram colhidas 70 dias após o plantio das mudas. A aplicação de esterco (dejetos de suínos ou esterco de aves) resultou em rendimento de couve equivalente à aplicação de fertilizante mineral, indicando que o requerimento de nutrientes das plantas foram totalmente atendidos pela aplicação de esterco animal. No entanto, a aplicação de dejetos de suínos resultou no aumento das concentrações de Fe, Mn, Zn, Cd, Cr e Pb nas folhas. A aplicação de N até a dose de 300 mg kg-1 indicou maior rendimento e melhor qualidade nutricional das folhas de couve. A aplicação de taxas elevadas de esterco, especialmente de dejeto suíno, implica no aumento da concentração de metais pesados nas folhas de couve. No entanto, as concentrações máximas obtidas de 0,042 mg kg-1 de Cd, 0,034 mg kg-1 de Cr e 0,030 mg kg-1 de Pb são muito inferiores ao limite máximo de consumo diário estabelecido pela legislação brasileira, indicando que o consumo de couve-de-folha oriunda dos sistemas de produção orgânicos com a aplicação de esterco animal pode ser considerado seguro e sem risco para a saúde humana.(AU)

Morfofisiologia de plantas de couve manteiga sob concentrações de húmus líquido / Morphophysiology of plants of butter collard as function of liquid humus concentration

A couve manteiga (Brassica oleracea var. acephala L.) pertence à família Brassicaceae e vários estudos têm demonstrado as variações ocorridas nas trocas gasosas entre as plantas e a atmosfera, em virtude de práticas ou de tratamentos aplicados às culturas agrícolas, com consequentes estados de estresse e reflexos negativos sobre os processos metabólicos das plantas. Além de ser uma opção para quem busca produção orgânica, o húmus líquido é uma estratégia econômica e eficiente que com inúmeros benefícios, tanto econômicos quanto agronômicos. Neste sentido, objetivou-se, avaliar algumas variáveis fisiológicas e morfológicas em plantas de couve, com concentrações de húmus líquido. O experimento foi conduzido em ambiente protegido na área experimental do setor de Horticultura e Controle Biológico pertencente à UNIOESTE, no município de Marechal Cândido Rondon, PR. Os tratamentos foram constituídos por cinco doses de húmus líquido (0, 5, 10, 15 e 20%). A adubação com o húmus líquido influenciou significativamente a fotossíntese líquida, concentração interna de CO2 e eficiência do uso da água. Massas secas de caule, folha e total não foram influenciadas pelos tratamentos. O número de folhas por planta e a área foliar foram afetadas pelas concentrações de húmus líquido. Apesar das variáveis transpiração e condutância estomática não terem apresentado diferença significativa, os demais parâmetros apresentaram resultados positivos em relação ao acréscimo de húmus líquido. Entretanto, o maior número de folhas por planta não influenciou na taxa de assimilação de CO2.(AU)

Brassica cabbage (Brassica oleracea var. Acephala L.) belongs to the Brassicaceae family and several studies have shown the variations in gas exchange between plants and the atmosphere, due to practices or treatments applied to agricultural crops, with consequent stress and negative reflexes on the metabolic processes of plants. In addition to being an option for those seeking organic production, liquid humus is an economic and efficient strategy with numerous benefits, both economic and agronomic. In this sense, the objective was to evaluate some physiological and morphological variables in cabbage plants, with concentrations of liquid humus. The experiment was carried out in a protected environment in the experimental area of Horticulture and Biological Control, belonging to UNIOESTE, in the city of Marechal Cândido Rondon, PR. The treatments consisted of five doses of liquid humus (0, 5, 10, 15, and 20%). Fertilization with liquid humus significantly influenced liquid photosynthesis, internal CO2 concentration and water use efficiency. Dry masses of stem, leaf, and total were not influenced by the treatments. The number of leaves per plant and the leaf area were affected by the concentrations of liquid humus. Although the variables transpiration and stomatal conductance did not present significant difference, the other parameters presented positive results in relation to the increase of liquid humus. However, the higher number of leaves per plant did not influence the assimilation rate of CO2.(AU)

Morfofisiologia de plantas de couve manteiga sob concentrações de húmus líquido / Morphophysiology of plants of butter collard as function of liquid humus concentration

A couve manteiga (Brassica oleracea var. acephala L.) pertence à família Brassicaceae e vários estudos têm demonstrado as variações ocorridas nas trocas gasosas entre as plantas e a atmosfera, em virtude de práticas ou de tratamentos aplicados às culturas agrícolas, com consequentes estados de estresse e reflexos negativos sobre os processos metabólicos das plantas. Além de ser uma opção para quem busca produção orgânica, o húmus líquido é uma estratégia econômica e eficiente que com inúmeros benefícios, tanto econômicos quanto agronômicos. Neste sentido, objetivou-se, avaliar algumas variáveis fisiológicas e morfológicas em plantas de couve, com concentrações de húmus líquido. O experimento foi conduzido em ambiente protegido na área experimental do setor de Horticultura e Controle Biológico pertencente à UNIOESTE, no município de Marechal Cândido Rondon, PR. Os tratamentos foram constituídos por cinco doses de húmus líquido (0, 5, 10, 15 e 20%). A adubação com o húmus líquido influenciou significativamente a fotossíntese líquida, concentração interna de CO2 e eficiência do uso da água. Massas secas de caule, folha e total não foram influenciadas pelos tratamentos. O número de folhas por planta e a área foliar foram afetadas pelas concentrações de húmus líquido. Apesar das variáveis transpiração e condutância estomática não terem apresentado diferença significativa, os demais parâmetros apresentaram resultados positivos em relação ao acréscimo de húmus líquido. Entretanto, o maior número de folhas por planta não influenciou na taxa de assimilação de CO2.

Brassica cabbage (Brassica oleracea var. Acephala L.) belongs to the Brassicaceae family and several studies have shown the variations in gas exchange between plants and the atmosphere, due to practices or treatments applied to agricultural crops, with consequent stress and negative reflexes on the metabolic processes of plants. In addition to being an option for those seeking organic production, liquid humus is an economic and efficient strategy with numerous benefits, both economic and agronomic. In this sense, the objective was to evaluate some physiological and morphological variables in cabbage plants, with concentrations of liquid humus. The experiment was carried out in a protected environment in the experimental area of Horticulture and Biological Control, belonging to UNIOESTE, in the city of Marechal Cândido Rondon, PR. The treatments consisted of five doses of liquid humus (0, 5, 10, 15, and 20%). Fertilization with liquid humus significantly influenced liquid photosynthesis, internal CO2 concentration and water use efficiency. Dry masses of stem, leaf, and total were not influenced by the treatments. The number of leaves per plant and the leaf area were affected by the concentrations of liquid humus. Although the variables transpiration and stomatal conductance did not present significant difference, the other parameters presented positive results in relation to the increase of liquid humus. However, the higher number of leaves per plant did not influence the assimilation rate of CO2.

Collard green yield and nutritional quality with mineral and organic fertilization / Produção e qualidade nutricional de couve-de-folha com adubação mineral e orgânica

A study was conducted to investigate the effect of organic and mineral fertilization on collard green yield, and to assess the macro and micronutrient and heavy metal concentrations of its leaves to assess the potential hazards of manure use. Collard plants (Brassica oleracea var. acephala, cv. couve-manteiga) were grown in 4-L pots filled with a clay soil under greenhouse conditions. The experimental design used was completely randomized design and the treatments in a 3 × 4 factorial arrangement: three N sources (pig slurry, poultry manure, and urea) and four N rates (0, 100, 200 and 300 mg kg-1), with four replicates. Collard greens leaves were harvested 70 days after seedling plantings. The manure application (pig slurry or poultry manure) resulted in collard yield equivalent to the application of mineral fertilizer. However, the pig slurry application resulted in an increase of leaf Fe, Mn, Zn, Cd, Cr, and Pb concentrations. Nitrogen application rates up to 300 mg N kg-1 result in the higher yield and nutritional quality of collard leaves. The application of high rates of manure, especially pig slurry, results in the increase of heavy metal (Cd, Cr, and Pb) concentrations. However, the maximum concentrations of 0.042 mg kg-1 Cd, 0.034 mg kg-1 Cr and 0.030 mg kg-1 Pb are well lower than maximum daily intake limit established by the Brazilian legislation, indicating that the consumption of collards from organic production systems with the manure application can be considered safe and without risk to human health.

Foi realizado um estudo para avaliar o efeito da aplicação de esterco e fertilizante mineral na produção e na qualidade nutricional de couve (Brassica oleracea var. acephala, cv. couve-manteiga). As plantas de couve foram cultivadas em vasos de 4 L preenchidos com um solo argiloso em condições de casa-de-vegetação. O delineamento experimental utilizado foi o inteiramente casualizado e os tratamentos em um arranjo fatorial 3 × 4: três fontes de N (dejetos de suínos, esterco de aves e ureia) e quatro doses de N (0, 100, 200 e 300 mg kg-1), com quatro repetições. As folhas de couve foram colhidas 70 dias após o plantio das mudas. A aplicação de esterco (dejetos de suínos ou esterco de aves) resultou em rendimento de couve equivalente à aplicação de fertilizante mineral, indicando que o requerimento de nutrientes das plantas foram totalmente atendidos pela aplicação de esterco animal. No entanto, a aplicação de dejetos de suínos resultou no aumento das concentrações de Fe, Mn, Zn, Cd, Cr e Pb nas folhas. A aplicação de N até a dose de 300 mg kg-1 indicou maior rendimento e melhor qualidade nutricional das folhas de couve. A aplicação de taxas elevadas de esterco, especialmente de dejeto suíno, implica no aumento da concentração de metais pesados nas folhas de couve. No entanto, as concentrações máximas obtidas de 0,042 mg kg-1 de Cd, 0,034 mg kg-1 de Cr e 0,030 mg kg-1 de Pb são muito inferiores ao limite máximo de consumo diário estabelecido pela legislação brasileira, indicando que o consumo de couve-de-folha oriunda dos sistemas de produção orgânicos com a aplicação de esterco animal pode ser considerado seguro e sem risco para a saúde humana.

Robust increase of leaf size by Arabidopsis thaliana GRF3-like transcription factors under different growth conditions.

An increase in crop yield is essential to reassure food security to meet the accelerating global demand. Several genetic modifications can increase organ size, which in turn might boost crop yield. Still, only in a few cases their performance has been evaluated under stress conditions. MicroRNA miR396 repress the expression of GROWTH-REGULATING FACTOR (GRF) genes that codes for transcription factors that promote organ growth. Here, we show that both Arabidopsis thaliana At-GRF2 and At-GRF3 genes resistant to miR396 activity (rGRF2 and rGRF3) increased organ size, but only rGRF3 can produce this effect without causing morphological defects. Furthermore, introduction of At-rGRF3 in Brassica oleracea can increase organ size, and when At-rGRF3 homologs from soybean and rice are introduced in Arabidopsis, leaf size is also increased. This suggests that regulation of GRF3 activity by miR396 is important for organ growth in a broad range of species. Plants harboring rGRF3 have larger leaves also under drought stress, a condition that stimulates miR396 accumulation. These plants also showed an increase in the resistance to virulent bacteria, suggesting that the size increment promoted by rGRF3 occurs without an obvious cost on plant defenses. Our findings indicate that rGRF3 can increase plant organ size under both normal and stress conditions and is a valuable tool for biotechnological applications.

Modified atmosphere in minimally processed cauliflower conservation and quality / Atmosfera modificada na conservação e qualidade da couve-flor minimamente processada

The objective of this work was to evaluate modified atmosphere effectiveness in minimally processed cauliflower aiming food safety maintenance for consumers, as conservation of product physical and chemical characteristics for better quality. Cauliflowers cv. Cindy were selected, washed, sanitized with sodium hypochlorite (200 mg L-1) and rinsed. After processing, they were immersed, one more time, in sodium hypochlorite solution (50 ppm) and dried by the air. Portions of 200 g were made and placed in different atmospheres: T1 = 0.05 % CO2 + 23 % O2; T2 = vacuum; T3 = tray of expanded polystyrene with polyvinyl chloride film (PVC) and T4 = 6 % CO2 + 4 % O2 + 90 % N2. The packages were stored in cold chamber at 5 ºC ± 1 and 85% ± 5 of relative humidity. Analysis were performed every two days and the evaluated traits were: loss of fresh mass, respiratory activity, soluble solids, titratable acidity, pH, reducing sugars, instrumental color, visual and microbiological analysis. The experimental design was completely randomized, in factorial design. Data were submitted to analysis of variance and means were compared using Tukey test at 5% of probability and regression analysis for storage period. Tray of expanded polystyrene with PVC delayed for two days product climacteric peak in relation to other modified atmosphere. Furthermore, it provided quality attributes maintenance. The best notes of visual appearance and the best intention of purchase were attributed to minimally processed cauliflower wrapped in modified atmosphere with 6% CO2. + 4% O2.(AU)

O objetivo deste trabalho foi avaliar a eficácia da utilização da atmosfera modificada em couve-flor minimamente processada, visando manter a segurança do alimento ao consumidor, além das suas características físico-químicas, mantendo sua qualidade. Foi utilizada couve-flor cv. Cindy, a qual foi selecionada, lavada, higienizada com hipoclorito de sódio (200 mg L-1) e enxaguada. Após o processamento, foi novamente imersa em solução de hipoclorito de sódio (50 ppm) e seca ao ar. Em seguida porções de 200 g foram acondicionadas em diferentes atmosferas, T1: 0,05 % CO2 + 23 % O2; T2: Vácuo; T3: Bandeja de poliestireno expandido com filme de policloreto de vinila (PVC) e T4: 6 % CO2 + 4 % O2 + 90 % N2. As embalagens foram armazenadas em câmara fria a 5±1 ºC e 85±5 % de umidade relativa. As análises foram realizadas a cada dois dias, quanto à perda de massa, atividade respiratória, sólidos solúveis, acidez titulável, pH, açúcares redutores, cor instrumental, análise visual e análise microbiológica. O delineamento experimental utilizado foi inteiramente casualizado (DIC), em esquema fatorial. Os dados foram submetidos à análise de variância e as médias foram comparadas por meio do teste de Tukey com 5 % de probabilidade, fez-se regressão para as análises no tempo de armazenamento. A bandeja de poliestireno expandido com filme de PVC atrasou por dois dias o pico climatérico do produto em relação às demais atmosferas modificadas, além de proporcionar a manutenção dos atributos de qualidade. As melhores notas de aparência visual e a melhor intenção de compra foram atribuídas para a couve-flor minimamente processada acondicionada na atmosfera modificada ativa com 6 % CO2. + 4 % O2.(AU)

Agronomic viability of New Zealand spinach and kale intercropping

ABSTRACT The intercropping is a production system that aims to provide increased yield with less environmental impact, due to greater efficiency in the use of natural resources and inputs involved in the production process. An experiment was carried out to evaluate the agronomic viability of kale and New Zealand spinach intercropping as a function of the spinach transplanting time. (0, 14, 28, 42, 56, 70, 84 and 98 days after transplanting of the kale). The total yield (TY) and yield per harvest (YH) of the kale in intercropping did not differ from those obtained in monoculture. The spinach TY was influenced by the transplanting time, the earlier the transplanting, the higher the TY. The spinach YH was not influenced by the transplanting time, but rather by the cultivation system. In intercropping, the spinach YH was 13.5% lower than in monoculture. The intercropping was agronomically feasible, since the land use efficiency index, which was not influenced by the transplanting time, had an average value of 1.71, indicating that the intercropping produced 71% more kale and spinach than the same area in monoculture. Competitiveness coefficient, aggressiveness and yield loss values showed that kale is the dominating species and spinach is the dominated.