ABSTRACT
Voltammetric scan can record the profile of electrochemical active substances in plant tissues. Because the distribution of chemical components in plants is controlled by genes, these profiles can reflect differences at the genetic level in different species. In this study, the voltammetric scan was applied to the investigation of macrophanerophytes taxonomy. All species of Chimonanthus with two exotaxa were deliberately selected due to their controversial infrageneric relationship. Electrode surface modification was excluded in this work to improve the convenience and accuracy of the fingerprint recording process. The dendrogram deduced from the electrochemical fingerprint data suggests that Ch. Zhejiangensis and Ch. grammatus are two groups of Ch. nitens, which may be only the ecotype of Ch. nitens, rather than independent taxonomic species. The small variations between the three species may be due to environmental factors and cannot be used for species formation. In addition, Ch. campanulatus and Ch. Praecox were clustered together with a close relationship.
Subject(s)
Calycanthaceae/chemistry , Plant Leaves/chemistry , Calycanthaceae/classification , Calycanthaceae/genetics , Electrochemical Techniques , Phylogeny , Plant Leaves/classification , Plant Leaves/geneticsABSTRACT
Glucose-6-phosphate dehydrogenase is main regulatory enzyme for pentose phosphate pathway. To amplify the core sequence of G6PDH gene from Chimonanthus praecox, the primers were synthesized, based on the conserved nucleotide sequence of other reported plant G6PDH genes. The specific primers were designed according to the major fragment. The full length cDNA of the G6PDH1 gene was isolated by the 3' and 5' rapid amplification of cDNA ends approach. Transcript levels of G6PDH1 isoform was measured by real-time quantitative RT-PCR in different tissues and in responds to cold treatment. The G6PDH1 subcellular localization, transmembrane domain, three-dimensional structure, and phylogenetic analysis were predicted by different software to analysis the bioinformatics of G6PDH1 protein. The G6PDH1 cDNA sequence was 2 011 bp in length and consisted of 1 551 bp Open Reading Frame (ORF) , encoding a protein of 516 amino acids. Expression analysis results in different tissues showed that G6PDH1 was primarily observed in flowers and roots, as opposed to the leaves and stems. Cold treatment experiments indicated that cold treatment caused a rapid increase in G6PDH1 expression in flowers within 12 h. The full-length cDNA of G6PDH1 and its expression analysis will play an important role for further study on cold stress responses in Ch. praecox.
Subject(s)
Calycanthaceae/enzymology , Cloning, Molecular , Glucosephosphate Dehydrogenase/genetics , Plant Proteins/genetics , Calycanthaceae/chemistry , Calycanthaceae/classification , Calycanthaceae/genetics , Enzyme Stability , Glucosephosphate Dehydrogenase/chemistry , Glucosephosphate Dehydrogenase/metabolism , Models, Molecular , Open Reading Frames , Phylogeny , Plant Proteins/chemistry , Plant Proteins/metabolismABSTRACT
OBJECTIVE: Species containing extremely aromatic compounds in leaves of Chimonanthus was analyzed to evaluate its genetic diversity and genetic relationships. METHOD: AFLP molecular marker technique was used in the study, UPGMA cluster analysis was conducted with the software of POPGENE32. RESULTS: Five hundred and fifty-nine bands were amplified by 10 pairs of primers screened, of which 226 bands were polymorphic, and the percentage of polymorphic bands was 36.8%. Observed number of alleles, effective number of alleles, Nei's genetic diversity index and Shannon's information index were 1.992 6, 1.306 5, 0.199 2 and 0.325 1, respectively. Genetic distances of the 21 populations were ranged from 0.039 2 to 0.289 4. CONCLUSION: Species containing extremely aromatic compounds in leaves of Chimonanthus with low genetic diversity play an important role in enhancing the protection of species and germplasm resources. Form the molecular level, the studies demonstrated the correctness of the result by Zhang Ruohui that species containing extremely aromatic compounds in leaves of Chimonanthus were divided into Ch. salicifolius, Ch. Zhejiangensis, Ch. nitens and Ch. grammatus.
Subject(s)
Calycanthaceae/genetics , Genetic Variation/genetics , Amplified Fragment Length Polymorphism Analysis , Calycanthaceae/classification , DNA, Plant/geneticsABSTRACT
OBJECTIVE: To classify the leaves of Chimonanthus nitens, Chimonanthus Salicifolius, Chimonanthus zhejiang and their fried samples, Discriminating equation was established to distinguish categories of raw materials. METHODS: The spectra of three kinds of Chimonanthus and fried samples were determined by FTIR. Principal component analysis, clustering analysis and discriminative analysis were applied to classify three kinds of Chimonanthus leaves and fried samples from different areas according to the relative absorbance of common peaks between 400 to 4000 cm(-1). RESULTS: 26 samples were divided into four classes, the discriminating accuracy was 96%. CONCLUSION: The method is rapid, simple and could be applied to evaluate the quality of Chimonanthus leaves without damaging to sample, and it is easy to identify the adulteration.