ABSTRACT
BACKGROUND: Adding resistant starch (RS) to bread formulations is a promising way of increasing fiber content of white bread. However, the partial replacement of wheat flour (WF) by RS can lead to a decrease in technological quality. The objective of this study was to analyze the performance of hydroxypropylmethylcellulose and carboxymethylcellulose as improvers of wheat bread with a high level of replacement (30%) with maize RS. The levels of the modified celluloses were 1% and 1.5% (WF + RS basis), and a formulation without modified celluloses was used as control. Proofing time, loaf volume, crumb characteristics (porosity, texture), and bread staling parameters (hardness increase, moisture loss), among other attributes, were analyzed, and principal component analysis was applied to compare samples. RESULTS: The use of both modified celluloses was effective in improving the quality of breads. Specific volume and crumb porosity were enhanced, particularly at the 1.5% level. Breads with modified celluloses also allowed a higher retention of water and a better preservation of mechanical properties during storage. The principal component analysis projection graph for the first two principal components showed that samples with modified celluloses were clustered by the level of hydrocolloid addition rather than by the type of hydrocolloid used, although all the samples with modified celluloses were close to each other and distant from the control sample without hydrocolloids. CONCLUSION: The quality decrease resulting from the replacement of WF by a high level of RS can be greatly compensated by the use of structuring agents such as hydroxypropylmethylcellulose and carboxymethylcellulose. © 2022 Society of Chemical Industry.
Subject(s)
Resistant Starch , Bread , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/chemistry , Triticum/chemistry , Colloids , Starch/chemistry , Hypromellose Derivatives/chemistryABSTRACT
Introdução: Estudos recentes apontam a utilização do curativo biológico com base em animais aquáticos como biomaterial na medicina regenerativa, apresentando boa aderência ao leito das feridas. O objetivo foi avaliar a eficácia da utilização da pele da Tilápia-do-Nilo (Oreochromis niloticus) como curativo biológico oclusivo, no manejo/tratamento de queimaduras de 2º grau em adultos. Métodos: Estudo clínico com 30 pacientes aleatoriamente tratados com pele da Tilápia-do-Nilo (n = 15) e hidrofibra com prata Aquacel Ag® (n =1 5). Resultados: Em relação à duração, o tratamento com a pele da Tilápiado-Nilo obteve uma média de dias de tratamento (9,6 ± 2,4) similar ao material comparativo (10,7 ± 4,5). Quanto ao relato de dor durante a troca de curativos, não houve diferença estatisticamente significante (p > 0,68) entre os grupos. Após a troca do curativo, não houve inferioridade no registro do valor na escala analógica de dor, em que 66,7% dos tratados com pele da Tilápia-do-Nilo relataram diminuição dos eventos álgicos. Constatou-se ainda que 60% dos pacientes tratados com a pele da Tilápia-do-Nilo não tiveram seus curativos substituídos em qualquer momento do tratamento. Para o curativo Aquacel AG®, 53,3% dos pacientes tiveram mais de uma substituição de curativos. Conclusões: Com base na pesquisa, pode-se concluir que a pele da Tilápia-do-Nilo é eficaz como curativo biológico oclusivo. Houve similaridade entre os grupos para a média de dias de tratamento (completa cicatrização da ferida) e para o relato de dor durante a realização do curativo. Também, a não inferioridade relacionada a dor após os curativos e suas trocas (quando existentes) e na quantidade de substituições destes.
Introduction: Recent studies have suggested the use of biological dressings made of aquatic animals as biomaterials in regenerative medicine since they demonstrate good adherence to the wound bed. The objective of this study was to evaluate the efficacy of Nile tilapia skin (Oreochromis niloticus) as an occlusive biological dressing in the management and treatment of second-degree burns in adults. Methods: This clinical study included 30 patients randomly treated with Nile tilapia skin (n = 15) or Aquacel Ag® silver-based hydrofiber dressing (n = 15). Results: The Nile tilapia skin yielded a similar mean treatment time (9.6 ± 2.4 days) to that of the comparative material (10.7 ± 4.5 days). There was no statistically significant intergroup difference (p > 0.68) in pain during dressing changes. No disadvantage in pain was noted, as 66.7% of patients treated with Nile Tilapia skin reported a decrease in pain events. Moreover, 60% of the patients treated with the Nile Tilapia skin did not require dressing replacement at any time during treatment. For the Aquacel AG® dressing, 53.3% of the patients required more than one dressing replacement. Conclusions: Our findings suggest that the Nile tilapia skin is as effective as an occlusive biological dressing. The average treatment time (complete wound healing) and pain reports during dressing changes were similar between groups. Furthermore, pain after and number of dressing exchanges (when performed) were not worse.
Subject(s)
Humans , Adult , Middle Aged , Wound Healing , Biological Dressings/adverse effects , Biological Dressings/standards , Burns/complications , Burns/diagnosis , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/adverse effects , Carboxymethylcellulose Sodium/therapeutic use , Skin Transplantation/adverse effects , Skin Transplantation/methods , Cichlids/injuries , Composite Tissue Allografts/physiopathology , Composite Tissue Allografts/injuries , Occlusive Dressings/adverse effects , Occlusive Dressings/standardsABSTRACT
A 14-year-old girl developed 4 episodes of anaphylaxis of unknown etiology, which required intramuscular adrenaline administration each time. She had eaten pizza and a cheeseburger immediately before the first 2 episodes, respectively, but had not eaten anything for several hours before the last 2 episodes. It turned out that she had eaten the same ice lolly 4 hours before the first 3 episodes and a Café au lait Swirkle (a half-frozen beverage) 4 hours before the last episode. We detected carboxymethylcellulose sodium as the only common ingredient in all anaphylactic episodes. Skin prick tests were positive for carboxymethylcellulose solution and carboxymethylcellulose-containing food products. We obtained a custom-made carboxymethylcellulose sodium-free ice lolly from the manufacturer and confirmed that it did not induce anaphylactic reactions by a challenge test. Carboxymethylcellulose, an anionic water-soluble polymer derived from native cellulose, is considered to be unabsorbable from the human gut and has been widely and increasingly used in pharmaceutical preparations, cosmetics, and food. This article is the first report of anaphylaxis caused by carboxymethylcellulose-containing foods, whereas anaphylaxis to carboxymethylcellulose has been rarely associated with carboxymethylcellulose-containing pharmaceuticals. Although the exact mechanisms underlying the induction of late-onset anaphylaxis by carboxymethylcellulose remain unclear, a small minority of cellulose-digesting microbial flora in the human colon and contamination of food products with carboxymethylcellulose of low molecular weight might be involved. The induction of recurrent anaphylaxis by various products should be a clue that prompts physicians to suspect food additives as a cause for anaphylaxis.
Subject(s)
Anaphylaxis/chemically induced , Anaphylaxis/diagnosis , Carboxymethylcellulose Sodium/adverse effects , Carboxymethylcellulose Sodium/analysis , Food Additives/adverse effects , Food Additives/analysis , Adolescent , Female , HumansABSTRACT
The addition of dietary fibers can alleviate the deteriorated textural properties and water binding capacity (WBC) that may occur when the fat content is lowered directly in the formulas of comminuted meat products. This study investigated the effects of the addition of chitosan or carboxymethyl cellulose (CMC) (2% w/w) to a model meat product. Both dietary fibers improved the water-binding capacity (WBC), while chitosan addition resulted in a firmer texture, CMC lowered the hardness. Chitosan addition resulted in a 2-fold reduction of lipid oxidation products, whereas CMC had no significant effect on oxidation. The effect of chitosan addition on lipid oxidation was evident both in the meat system and after simulated in vitro gastrointestinal digestion. Low-field nuclear magnetic resonance (NMR) relaxometry revealed that the fibers impacted the intrinsic water differently; the addition of chitosan resulted in a faster T2 relaxation time corresponding to water entrapped in a more dense pore network. Coherent anti-Stokes Raman scattering (CARS) microscopy was for the first time applied in a meat product to study the microstructure, which revealed that the two fibers exerted different effects on the size and entrapment of fat droplets in the protein network, which probably explain the mechanisms by which chitosan reduced lipid oxidation in the system.
Subject(s)
Carboxymethylcellulose Sodium/analysis , Chitosan/analysis , Food Additives/chemistry , Meat Products/analysis , Animals , Food Handling , Oxidation-Reduction , Swine , Water/analysisABSTRACT
The understanding of the interactions between starch and cellulose hydrocolloids is crucial for equipment design and definition of operation parameters in the food industry. In the present study, the possible interactions of wheat starch (WS) with different levels (2-10%) of carboxymethyl cellulose (CMC) or microcrystalline cellulose (MCC) in short-term retrogradation were explored by dynamic rheological and Fourier transform infrared spectrum (FTIR) measurements. The chains of water-soluble CMC could creep into the continuous phase during paste gelation. It was found the amylose network structure was broken and the inter- and intra-molecular hydrogen bond of WS were weaken after gelation. Moreover, there were interactions between the chains of CMC and chains of amylose at higher CMC concentration. On other hand, water connected with COO- groups blocked the interactions between CMC chains and amylose, and the interactions among CMC chains. The amorphous phases of high crystalline MCC were just swollen during paste gelation. It results that heterogeneous microstructure with amylose and MCC domains unevenly distributed was in WS/MCC paste.
Subject(s)
Carboxymethylcellulose Sodium/chemistry , Cellulose/chemistry , Starch/chemistry , Triticum/chemistry , Carboxymethylcellulose Sodium/analysis , Cellulose/analysis , Colloids/analysis , Colloids/chemistry , Rheology , Spectroscopy, Fourier Transform InfraredABSTRACT
One of the main obstacles to the successful treatment of tuberculosis is the poor and variable oral bioavailability of rifampicin (RIF), which is mainly due to its low hydrophilicity and dissolution rate. The aim of this work was to obtain a hydrophilic new material that allows a very fast dissolution rate of RIF and therefore is potentially useful in the development of oral solid dosage forms. The acid form of carboxymethylcellulose (CMC) was co-processed with RIF by solvent impregnation to obtain CMC-RIF powder, which was characterized by polarized optical microscopy, powder x-ray diffraction, DSC-TGA, hot stage microscopy, 13C and 15N solid-state NMR and FT-IR spectroscopy. In addition, the CMC-RIF matrices were subjected to water uptake and dissolution studies to assess hydrophilicity and release kinetics. CMC-RIF is a crystalline solid dispersion. Solid-state characterization indicated that no ionic interaction occurred between the components, but RIF crystallized as a zwitterion over the surface of CMC, which drastically increased the hydrophilicity of the solid. The CMC-RIF matrices significantly improved the water uptake of RIF and disintegrated in a very short period immediately releasing RIF. As CMC improves the hydrophilicity and delivery properties of RIF, CMC-RIF is very useful in the design of oral solid dosage forms with very fast dissolution of RIF, either alone or in combination with other antitubercular drugs.
Subject(s)
Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/chemistry , Rifampin/analysis , Rifampin/chemistry , Calorimetry, Differential Scanning/methods , Magnetic Resonance Spectroscopy/methods , Solubility , Spectroscopy, Fourier Transform Infrared/methods , Time Factors , X-Ray Diffraction/methodsABSTRACT
Characteristics of the buccal mucoadhesive films (film thickness, film weight, uniformity of mass and moisture content) prepared by solvent casting method were tested in this experimental study. The formulations consisted either of one mucoadhesive polymer (sodium hyaluronate of two different molecular weights and sodium carboxymethylcellulose) or combinations thereof. On the basis of the aforementioned tests, it was determined that water content was influenced by the molecular weight of sodium hyaluronate as well as by the ratio of mucoadhesive polymers in the composition. The composition of the films influences also other tested parameters.Key words: buccal mucoadhesive films solvent casting method sodium hyaluronate sodium carboxymethylcellulose water content.
Subject(s)
Hyaluronic Acid/chemistry , Mouth Mucosa/chemistry , Carboxymethylcellulose Sodium/analysis , Solvents , Water/analysisABSTRACT
Alpha-cellulose extracted from corn husks was used as the raw material for the production of food-grade carboxymethyl cellulose (CMC). Preparation of CMC from husk cellulose was carried out by an etherification process, using sodium hydroxide and monochloroacetic acid (MCA), with ethanol as the supporting medium. Characterizations of CMC were carried out by analyzing the spectra of FTIR, XRD patterns and SEM photomicrographs. Degree of substitution (DS) was determined with respect to particle size using chemical methods. Solubility, molecular weight and DS of CMC increased with decreased cellulose particle sizes. Microbiological testing of the prepared CMC was done by the pour plate method. Concentrations of heavy metals such as arsenic, lead, cadmium and mercury in the purified CMC were measured by Atomic Absorption Spectroscopy technique and found to be within the WHO/FAO recommended value. A comparative study with CMC available in the international market was conducted. The purity of the prepared CMC was higher, at 99.99% well above the purity of 99.5% for standard CMC. High purity CMC showed a yield 2.4 g/g with DS 2.41, water holding capacity 5.11 g/g, oil holding capacity 1.59 g/g. The obtained product is well suited for pharmaceutical and food additives.
Subject(s)
Carboxymethylcellulose Sodium/chemistry , Cellulose/chemistry , Zea mays/chemistry , Acetates/chemistry , Arsenic/analysis , Cadmium/analysis , Carboxymethylcellulose Sodium/analysis , Cellulose/isolation & purification , Ethanol/chemistry , Food Analysis , Food Industry/methods , Humans , Lead/analysis , Mercury/analysis , Molecular Weight , Sodium Hydroxide/chemistry , Solubility , Waste Products , WaterABSTRACT
In the present study, functionalization of mixed office waste (MOW) paper has been carried out to synthesize carboxymethyl cellulose, a most widely used product for various applications. MOW was pulped and deinked prior to carboxymethylation. The deinked pulp yield was 80.62 ± 2.0% with 72.30 ± 1.50% deinkability factor. The deinked pulp was converted to CMC by alkalization followed by etherification using NaOH and ClCH2COONa respectively, in an alcoholic medium. Maximum degree of substitution (DS) (1.07) of prepared CMC was achieved at 50 °C with 0.094 M and 0.108 M concentrations of NaOH and ClCH2COONa respectively for 3h reaction time. The rheological characteristics of 1-3% aqueous solution of optimized CMC product showed the non-Newtonian pseudoplastic behavior. Fourier transform infra red (FTIR), nuclear magnetic resonance (NMR) and scanning electron microscope (SEM) study were used to characterize the CMC product.
Subject(s)
Carboxymethylcellulose Sodium/analysis , Paper , Recycling/methods , Waste Management/methods , Carboxymethylcellulose Sodium/chemistryABSTRACT
OBJECTIVES: The aim of the study was to use methods of pharmaceutical technology, and prepare carriers in the form of pellets suitable as a filling of detection tubes for enzymatic detection of cholinesterase inhibitors. The enzymatic detection was based on enzymatic hydrolysis of acetylthiocholine iodide and the subsequent colour reaction of its hydrolysis product with Ellman's reagent. The suitable carriers should be in the form of white, regular and sufficiently mechanically resistant particles of about 1 mm allowing it to capture the enzyme during the impregnation process and ensuring its high activity for enzymatic detection. METHODS: Carriers consisting of microcrystalline cellulose, lactose, povidone, and sodium carboxymethyl cellulose were prepared using extrusion-spheronization method under three different drying conditions in either a hot air oven or a microwave oven. Subsequently, the carriers were impregnated with acetylcholinesterase and their size, shape, mechanical resistance, bulk, tapped and pycnometric density, Hausner ratio, intraparticular and total tapped porosity, and activity were measured and recorded. RESULTS: In this procedure, carriers with different physical parameters and different acetylcholinesterase activity were evaluated. It was found that higher acetylcholinesterase activity was associated not only with a higher intraparticular porosity but also with more regular particles characterized by high sphericity and low total tapped porosity. CONCLUSION: This unique finding is important for the preparation of detection tubes based on enzymatic detection which is still irreplaceable especially in the field of detection and analysis of super-toxic cholinesterase inhibitors.
Subject(s)
Carboxymethylcellulose Sodium/analysis , Cellulose/analysis , Cholinesterase Inhibitors/metabolism , Lactose/analysis , Povidone/analysis , Acetylthiocholine/analogs & derivatives , Acetylthiocholine/metabolism , Dithionitrobenzoic Acid , Materials Testing , Porosity , Sulfhydryl ReagentsABSTRACT
The application of twin screw granulation in the pharmaceutical industry has generated increasing interest due to its suitability for continuous processing. However, an understanding of the impact of formulation properties such as hydrophobicity on intermediate and finished product quality has not yet been established. Hence, the current work investigated the granulation behaviour of three formulations containing increasing amounts of hydrophobic components using a Consigma™-1 twin screw granulator. Process conditions including powder feed rate, liquid to solid ratio, granulation liquid composition and screw configuration were also evaluated. The size of the wet granules was measured in order to enable exploration of granulation behaviour in isolation without confounding effects from downstream processes such as drying. The experimental observations indicated that the granulation process was not sensitive to the powder feed rate. The hydrophobicity led to heterogeneous liquid distribution and hence a relatively large proportion of un-wetted particles. Increasing numbers of kneading elements led to high shear and prolonged residence time, which acted to enhance the distribution of liquid and feeding materials. The bimodal size distributions considered to be characteristic of twin screw granulation were primarily ascribed to the breakage of relatively large granules by the kneading elements.
Subject(s)
Drug Compounding/instrumentation , Excipients/chemistry , Placebos/chemistry , Calcium Phosphates/analysis , Calcium Phosphates/chemistry , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/chemistry , Cellulose/analysis , Cellulose/chemistry , Chemical Phenomena , Chemistry, Pharmaceutical , England , Excipients/analysis , Hydrophobic and Hydrophilic Interactions , Lactose/analysis , Lactose/chemistry , Materials Testing , Particle Size , Quality Control , Reproducibility of Results , Surface Properties , Time Factors , Water/analysisABSTRACT
PURPOSE: The purpose of this work was to gather preliminary data on tear film stability, and the adhesive properties of the corneal surface in dry eye patients and control group subjects, using a new, minimally invasive optical coherence tomography (OCT) imaging method. METHODS: We screened 85 human subjects for dry eye and classified them in two groups, as dry eye or normal patients. Sodium carboxymethylcellulose (NaCMC) adhesiveness over the central cornea was measured using Fourier domain anterior segment OCT. The corneal adhesiveness for NaCMC was compared between the two groups, correlated with classical tests, and analyzed for diagnostic validity and repeatability. RESULTS: The corneal adhesiveness for NaCMC median and mode values was fair (between 1 and 3 minutes) for dry eye subjects (n = 36) and borderline (between 3 and 5 minutes) for control group subjects (n = 49), and was significantly different between the two groups (P < 0.001). Significant correlations were observed between the corneal adhesiveness measures and dry eye patients' symptoms (P < 0.001), Schirmer I test (P < 0.001), ocular surface staining (P < 0.001), and, particularly, fluorescein break-up time (P < 0.001). The area under the receiver operating characteristic curve was 0.94 (P < 0.001), suggesting reliable sensitivity and specificity of OCT imaging. A statistically significant intraclass correlation (ICC) value of 0.99 was found for measurements of corneal adhesiveness on two subsequent days at the same time (P < 0.001). CONCLUSIONS: This minimally invasive, novel technique of OCT imaging of the corneal surface following NaCMC drop instillation provides a measure of corneal adhesiveness. This technique may improve the clinician's ability in the understanding and diagnosis of the dry eye syndrome.
Subject(s)
Carboxymethylcellulose Sodium/analysis , Cornea/chemistry , Dry Eye Syndromes/diagnosis , Tomography, Optical Coherence/methods , Adhesiveness , Cornea/pathology , Dry Eye Syndromes/metabolism , Female , Humans , Male , Middle Aged , ROC Curve , Reproducibility of Results , Surface Properties/drug effectsABSTRACT
The impact of filler-lubricant particle size ratio variation (3.4-41.6) on the attributes of an immediate-release tablet was compared with the impacts of the manufacturing method used (direct compression or dry granulation) and drug loading (1%, 5%, and 25%), particle size (D[4,3]: 8-114 µm), and drug type (theophylline or ibuprofen). All batches were successfully manufactured, except for direct compression of 25% drug loading of 8 µm (D[4,3]) drug, which exhibited very poor flow properties. All manufactured tablets possessed adequate quality attributes: tablet weight uniformity <4% RSD, tablet potency: 94%-105%, content uniformity <6% RSD, acceptance value ≤ 15, solid fraction: 0.82-0.86, tensile strength >1 MPa, friability ≤ 0.2% weight loss, and disintegration time < 4 min. The filler-lubricant particle size ratio exhibited the greatest impact on blend and granulation particle size and granulation flow, whereas drug property variation dominated blend flow, ribbon solid fraction, and tablet quality attributes. Although statistically significant effects were observed, the results of this study suggest that the manufacturability and performance of this immediate-release tablet formulation is robust to a broad range of variation in drug properties, both within-grade and extra-grade excipient particle size variations, and the choice of manufacturing method.
Subject(s)
Chemistry, Pharmaceutical/methods , Drug Compounding/methods , Excipients/analysis , Tablets/analysis , Carboxymethylcellulose Sodium/analysis , Cellulose , Chemistry, Pharmaceutical/standards , Drug Compounding/standards , Excipients/standards , Hardness , Ibuprofen/administration & dosage , Ibuprofen/analysis , Lactose , Lubricants , Particle Size , Powders , Pressure , Solubility , Stearic Acids/analysis , Tablets/standards , Theophylline/administration & dosage , Theophylline/analysisABSTRACT
For centuries silver and silver compounds have been in use to control infection and avoid septicaemia in the care of burns and chronic wounds. Renewed interest has resulted in a number of Ag based dressings that are now widely used in burns centres. Despite extensive use, a systematic study of the chemical composition, release kinetics and biochemical action of these products has yet to be published. In this work we have characterized the morphology of four commercial Ag dressings by scanning electron microscopy and the silver content was determined to range between 1.39 mg/cm(2) and 0.03 mg/cm(2). Release kinetics in three different matrices (ultra pure water, normal saline solution and a human serum substitute) were determined. The highest rates were found in serum substitute, with a maximum of 4099 µg/(hcm(2)) to a minimum of 0.0001 µg/(hcm(2)). Our results show that the mean inhibitory concentrations are exceeded for most common pathogens in serum substitute and sterile water, but the presence of high Cl(-) concentrations tend to inactivate the dressings.
Subject(s)
Bandages , Burns/therapy , Silver/analysis , Bandages/standards , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/chemistry , Microscopy, Electron, Scanning , Polyesters/analysis , Polyesters/chemistry , Polyethylenes/analysis , Polyethylenes/chemistry , Serum/chemistry , Sodium Chloride/chemistry , Water/chemistryABSTRACT
Physiological studies were conducted to determine the optimum cultural conditions for maximal carboxymethyl cellulase (CMCase) formation by Aspergillus terreus DSM 826. Shaking condition at 150 rpm is favorable for the production of CMCase from rice straw and sugar cane bagasse. The highest enzyme yield was obtained at the third day of incubation at 30ºC for both cases; however CMCase formation occurred at a broad range of pH values, with maximal formation of A. terreus DSM 826 CMCase at pH 4.5 and 5.0 when rice straw and sugar cane bagasse were used as sole carbon source, respectively. Carboxymethyl cellulose (CMC) was found to be a good inducer for CMCase formation in both agricultural wastes with CMC concentrations of 0.5 and 1.0 % (w/v) in case of rice straw and sugar cane bagasse, respectively. High level of enzyme formation was obtained with the addition of ammonium chloride as nitrogen source in both cases and at a concentration of 0.4 % (v/v Tween-80) as an addition to medium containing rice straw. However this addition did not influence the production of CMCase in case of using sugar cane bagasse as carbon source.
Subject(s)
Aspergillus/isolation & purification , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/isolation & purification , Plant Structures/enzymology , Oryza/enzymology , Saccharum/enzymology , Enzyme Activation , Food Samples , Methodology as a SubjectABSTRACT
An extracellular endoglucanase was isolated from the culture liquid of xylanase producing strain Aspergillus niger B03. The enzyme was purified to a homogenous form, using consecutive ultrafiltration, anion exchange chromatography, and gel filtration. Endoglucanase was a monomer protein with a molecular weight of 26,900 Da determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 28,800 Da determined by gel filtration. The optimal pH and temperature values for the enzyme action were 3.5 and 65ºC respectively. Endoglucanase was stable at 40ºC, pH 3.0 for 210 min. The substrate specificity of the enzyme was determined with carboxymethyl cellulose, filter paper, and different glycosides. Endoglucanase displayed maximum activity in the case of carboxymethyl cellulose, with a Km value of 21.01 mg/mL. The substrate specificity and the pattern of substrate degradation suggested that the enzyme is an endoglucanase. Endoglucanase showed a synergism with endoxylanase in corn cobs hydrolysis.
Subject(s)
Aspergillus niger/enzymology , Aspergillus niger/isolation & purification , Chromatography, Gel , Carboxymethylcellulose Sodium/analysis , Glycosides , Xylans/analysis , Electrophoresis , Enzyme Activation , MethodsABSTRACT
A cellulolytic bacterial strain, designated P118, isolated from the gut of the tropical fish Parotocinclus maculicauda was identified as belonging to the genus Paenibacillus based on phenotypic and chemotaxonomic characteristics and the 16S rRNA gene sequence. The novel strain was Gram-positive, spore-forming and rod-shaped. Catalase but not oxidase was produced. Carboxymethylcellulose was hydrolyzed but starch or gelatin was not. Acetoin production was negative whereas nitrate reduction and urease production were positive. Many carbohydrates served as carbon sources for growth. MK-7 was the predominant isoprenoid quinone. Anteiso-C15:0 (38.73 percent) and C16:0 (20.85 percent) were the dominant cellular fatty acids. Strain P118 was closely related to Paenibacillus amylolyticus NRRL NRS-290, P. pabuli HSCC 492, P. tundrae Ab10b, P. xylanexedens B22a, and P. tylopili MK2 with 98.3-98.8 percent 16S rRNA gene sequence similarity. The results presented here suggest that strain P118 represents a novel species of the genus Paenibacillus and it is a potential strain for further studies concerning its role in the production of industrially important products from cellulosic biomass.
Subject(s)
Animals , Biomass , Bacillus/isolation & purification , Catfishes , Chemotactic Factors , Carboxymethylcellulose Sodium/analysis , Catalase/isolation & purification , Oxidoreductases , Phenotype , Methods , MethodsABSTRACT
The cellulase production by Trichoderma viride, cultivated on different substrates, namely steam-pretreated Lespedeza, filter paper, microcrystalline cellulose (MCC) or carboxymethyl cellulose (CMC), was studied. Different cellulase systems were secreted when cultivated on different substrates. The cellulolytic enzyme from steam-pretreated Lespedeza medium performed the highest filter paper activity, exoglucanase and endoglucanase activities, while the highest ß-glucosidase activity was obtained from the enzyme produced on filter paper medium. The hydrolytic potential of the enzymes produced from different media was evaluated on steam-pretreated Lespedeza. The cellulase from steam-pretreated Lespedeza was found to have the most efficient hydrolysis capability to this specific substrate. The molecular weights of the cellulases produced on steam-pretreated Lespedeza, filter paper and MCC media were 33, 37 and 40 kDa, respectively, and the cellulase from CMC medium had molecular weights of 20 and 43 kDa. The degree of polymerization, crystallinity index and micro structure scanned by the scanning electron microscopy of degraded steam-pretreated Lespedeza residues were also studied.
Subject(s)
Cellulases/metabolism , Cellulose/analysis , Cellulose/metabolism , Lespedeza/chemistry , Trichoderma/enzymology , Carbohydrates/chemistry , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/metabolism , Cellulase/analysis , Cellulase/biosynthesis , Cellulase/metabolism , Cellulases/biosynthesis , Cellulose/ultrastructure , Glucan 1,3-beta-Glucosidase/analysis , Glucan 1,3-beta-Glucosidase/biosynthesis , Glucan 1,3-beta-Glucosidase/metabolism , Hydrolysis , Lespedeza/metabolism , Lespedeza/ultrastructure , Paper , Polymerization , Steam , Substrate Specificity , Trichoderma/metabolism , X-Ray Diffraction/methods , beta-Glucosidase/analysis , beta-Glucosidase/biosynthesis , beta-Glucosidase/metabolismABSTRACT
PURPOSE OF WORK: To show biological activity of carboxymethylated α-(1 â 3)-D-glucans isolated from the selected macromycetes fungi on human tumor and normal cells. Water-insoluble, alkali-soluble polysaccharides (WIP) were isolated from fruiting bodies of four macromycetes fungi: Lentinus edodes, Pleurotus ostreatus, Piptoporus betulinus and Laetiporus sulphureus. The structure of the polysaccharides was determined using composition analysis, methylation analysis, fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. The chemical and spectroscopic investigations indicated that the polysaccharides were an α-(1 â 3)-D-glucans. A biological activity analysis of the carboxymethylated (CM) α-(1 â 3)-D-glucans was based on an assessment of their cytotoxic, mitochondrial metabolism-modulating, and free radical scavenging effects. The cytotoxic activity of the CM-glucans was concentration- and cell-type-dependent. The tested CM-glucans, generally, did not have a free radical scavenging effect. The CM-α-(1 â 3)-D-glucans isolated from the selected macromycetes fungi are biologically active and may therefore be used as diet or therapy supplements.
Subject(s)
Basidiomycota/chemistry , Carboxymethylcellulose Sodium/analysis , Carboxymethylcellulose Sodium/pharmacology , Fruiting Bodies, Fungal/chemistry , Glucans/analysis , Cell Line, Tumor , Cell Survival/drug effects , HumansABSTRACT
The aim of the present study was to evaluate the effects of experimental saliva substitutes based on carboxymethyl cellulose (CMC) differing in degrees of saturation with respect to calcium phosphates on the mineral loss of enamel in vitro. Demineralized bovine specimens (subsurface lesions) were exposed to one of six experimental CMC-based solutions with theoretical degrees of saturation with respect to octacalcium phosphate (S(OCP)) of S0, S0.5, S1, S2, S4, and S8 for 10 weeks. A previously studied saliva substitute (Glandosane) and two aqueous solutions (C0 and C1) served as controls. Mineral losses and lesion depths before and after storage were evaluated from microradiographs. Free and bound calcium as well as phosphate and fluoride concentrations were determined. According to these measurements, S(OCP) of S2, S4, and S8 was 0.3, 1.1, and 3.4, respectively. Storage in Glandosane and both negative controls resulted in significant demineralization (p < 0.05). Only S2 significantly remineralized the specimens (p < 0.05). All other solutions showed neutral effects. No significant differences in mineralization between S0 and C0 as well as between S1 and C1 could be observed (p > 0.05). It can be concluded that a CMC-based solution actually unsaturated with respect to octacalcium phosphate (S2) shows most pronounced remineralization capability under the conditions chosen. This might be explained by a more favorable balance between calcium bound to CMC in an adsorbed layer at the enamel-liquid interface and heterogeneous nucleation of calcium phosphates within a solution compared to solutions either supersaturated or having lower levels of saturation.