Subject(s)
Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Diagnosis, Differential , Heparin/administration & dosage , Heparin/therapeutic use , Puerperal Infection , Signs in Homeopathy , Ampicillin/therapeutic use , Carbenicillin/therapeutic use , Cephaloridine/therapeutic use , Chloramphenicol/therapeutic use , Clindamycin/therapeutic use , Gentamicins/therapeutic use , Kanamycin/therapeutic use , Metronidazole/therapeutic use , Penicillin G/therapeutic use , Tetracycline/therapeutic useABSTRACT
Se describe un método inmunoenzimático, que, por su gran sensibilidad, permite detectar pequeñas cantidades de proteína A presente en la membrana celular de determinadas cepas de Staphylococcus aureus o la liberada en el medio de cultivo. Esta técnica del dot-enzimoinmunoensayo permite poner en evidencia concentraciones de hasta 0,115 ug.ml-1 de proteína. Comparando los resultados obtenidos con la técnica de inmunodifusión radial, se confirma su mayor sensibilidad y la posibilidad de utilizar un método específico y de fácil realización en el dosaje de proteína A.
Subject(s)
Immunoassay , In Vitro Techniques , Staphylococcal Protein A/analysis , Staphylococcus aureus/analysis , Bacteriological Techniques , Cephaloridine , Culture MediaABSTRACT
Se describe un método inmunoenzimático, que, por su gran sensibilidad, permite detectar pequeñas cantidades de proteína A presente en la membrana celular de determinadas cepas de Staphylococcus aureus o la liberada en el medio de cultivo. Esta técnica del dot-enzimoinmunoensayo permite poner en evidencia concentraciones de hasta 0,115 ug.ml-1 de proteína. Comparando los resultados obtenidos con la técnica de inmunodifusión radial, se confirma su mayor sensibilidad y la posibilidad de utilizar un método específico y de fácil realización en el dosaje de proteína A. (AU)
Subject(s)
In Vitro Techniques , Staphylococcus aureus/analysis , Immunoassay/methods , Staphylococcal Protein A/analysis , Culture Media , Cephaloridine , Bacteriological TechniquesABSTRACT
Se realiza un estudio de 93 muestras procedentes de pacientes com meningoencefalitis y meningococcemia a los cuales se les realizó toma de muestra de líquido cefaloraquídeo (LCR) y hemocultivo respectivamente. Dichas muestras se procesaron según las Normas Técnicas de Microbiología realizándose el aislamiento y caracterización de los microorganismos incidentes, demonstrándose que el 82,79% de los gérmenes aislados correspondieron a Neisseria meningitidis y de ellas el Serogrupo B, Serotipo 15 fue el de más alto índice (93,5%). Analizando los resultados de los antibiogramas por difusión de estas cepas, se pudo demostrar que la mayor sensibilidad correspondió al cloranfenicol (96,1%) y el mayor valor de resistencia fue para la Cefaloridina (36,36%)
Subject(s)
Infant , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Humans , Male , Female , Meningitis, Meningococcal/microbiology , Meningoencephalitis/microbiology , Neisseria meningitidis/isolation & purification , Drug Resistance, Microbial , Cephaloridine , Chloramphenicol , CubaABSTRACT
The effect of prophylactic antibiotics on bacterial colonization of the respiratory tract and on general progression of cystic fibrosis was studied in a two-year prospective study of 47 mildly to moderately affected patients. One group of patients received inhaled cephaloridine and the other received no inhaled antibiotic; both groups received cloxacillin orally. Carriage of Haemophilus influenzae was greater in the group not receiving inhaled antibiotic (55% vs 20%). Rates of carriage of Staphylococcus aureus (23%). Pseudomonas aeruginosa (greater than 90%). Pseudomonas cepacia (45%), and other organisms were similar in both groups. There were no significant differences between the two groups in incidence of respiratory tract infections or hospital admissions, clinical scores, radiologic scores, or rate of change of pulmonary function. Although continuous antistaphylococcal antibiotic prophylaxis may be successful in suppressing colonization with S. aureus, it may also contribute to the high rates of carriage of Ps. aeruginosa and Ps. cepacia observed in patients with cystic fibrosis.
Subject(s)
Cephaloridine/therapeutic use , Cloxacillin/therapeutic use , Cystic Fibrosis/therapy , Respiratory Tract Infections/prevention & control , Administration, Oral , Adolescent , Aerosols , Cephaloridine/administration & dosage , Child , Cloxacillin/administration & dosage , Female , Haemophilus influenzae/isolation & purification , Humans , Male , Prospective Studies , Pseudomonas/isolation & purification , Respiratory Function Tests , Sputum/microbiology , Staphylococcus aureus/isolation & purificationSubject(s)
Child, Preschool , Child , Adolescent , Adult , Middle Aged , Humans , Male , Female , Cephaloridine , Eye DiseasesABSTRACT
Pharmacologic data are presented on 39 children treated for osteomyelitis with one of the following antibiotics: methicillin, dicloxacillin, cephaloridine, or cefazolin. The concentrations of drug in pus and bone were correlated with serum concentrations, with the susceptibilities of Staphylococcus aureus strains isolated from the patients, and with the degree of drug protein-binding. The penetration of the antibiotics into pus and bone was similar for the two penicillins and for the two cephalosporins despite the disparate protein-binding affinities of these drugs. The agents attained concentrations in tissues that were at least several fold, and often more than tenfold, greater than the MIC and MBC values of the S. aureus stains. These data provide a basis for selection of antimicrobial agents for treatment of osteomyelitis.
Subject(s)
Anti-Bacterial Agents/metabolism , Bone and Bones/metabolism , Osteomyelitis/metabolism , Suppuration/metabolism , Adolescent , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/therapeutic use , Cefazolin/metabolism , Cephaloridine/metabolism , Child , Child, Preschool , Dicloxacillin/metabolism , Female , Humans , Male , Methicillin/metabolism , Osteomyelitis/drug therapy , Osteomyelitis/microbiology , Protein Binding , Staphylococcus aureus/drug effectsSubject(s)
Peritonitis/therapy , Therapeutic Irrigation/methods , Adolescent , Adult , Aged , Cephaloridine/therapeutic use , Child , Child, Preschool , Female , Humans , Male , Middle Aged , Peritoneal Cavity , Solutions , Therapeutic Irrigation/adverse effectsABSTRACT
One hundred and one strains of Hemophilus influenzae type b isolated at the Children's Hospital Medical Center from blood or CSF and 18 strains known to be resistant to ampicillin of which 17 were supplied to us by others were tested for their sensitivity to 17 antibiotics. Two groups were defined according to their sensitivity to ampicillin. When 10-3 bacteria were applied, 18 strains isolated from patients with ampicillin treatment failures had a median minimum inhibitory concentrations of 3.1 mug/ml. At 10-6 bacteria the median MIC for the resistant strains increased 512-fold, whereas the sensitive strains increased two-fold. The most active antibiotics against sensitive and resistant strains were rifampin, chloramphenicol, gentamicin, rosamycin, and erythromycin with MICs ranging from 0.2-0.8 mug/ml at 10-3 bacteria and from 0.2-1.6 mug/ml at 10-6.