ABSTRACT
Satellite DNA (satDNA) is one of the major fractions of the eukaryotic nuclear genome. Highly variable satDNA is involved in various genome functions, and a clear link between satellites and phenotypes exists in a wide range of organisms. However, little is known about the origin and temporal dynamics of satDNA. The "library hypothesis" indicates that the rapid evolutionary changes experienced by satDNAs are mostly quantitative. Although this hypothesis has received some confirmation, a number of its aspects are still controversial. A recently developed next-generation sequencing (NGS) method allows the determination of the satDNA landscape and could shed light on unresolved issues. Here, we explore low-coverage NGS data to infer satDNA evolution in the phylogenetic context of the diploid species of the Chenopodium album aggregate. The application of the Illumina read assembly algorithm in combination with Oxford Nanopore sequencing and fluorescent in situ hybridization allowed the estimation of eight satDNA families within the studied group, six of which were newly described. The obtained set of satDNA families of different origins can be divided into several categories, namely group-specific, lineage-specific and species-specific. In the process of evolution, satDNA families can be transmitted vertically and can be eliminated over time. Moreover, transposable element-derived satDNA families may appear repeatedly in the satellitome, creating an illusion of family conservation. Thus, the obtained data refute the "library hypothesis", rather than confirming it, and in our opinion, it is more appropriate to speak about "the library of the mechanisms of origin".
Subject(s)
Chenopodium album/genetics , DNA, Plant/analysis , DNA, Satellite/analysis , Diploidy , Evolution, Molecular , Genome, Plant , Chenopodium album/growth & development , DNA, Plant/genetics , DNA, Satellite/genetics , Gene Library , High-Throughput Nucleotide Sequencing , Phylogeny , Species SpecificityABSTRACT
MAIN CONCLUSION: Detrimental pleiotropic effects of resistance mutation(s) were observed for multiple-resistant phenotypes (resistant to both atrazine and dicamba). The multiple-resistant phenotypes had lower growth rates and less capacity for vegetative growth compared to the phenotypes only resistant to atrazine. The fitness costs that are conferred by herbicide resistance alleles can affect the rate of herbicide resistance evolution within populations. We evaluated the direct fitness costs involved with multiple resistance to dicamba and atrazine (R1 and R2) in Chenopodium album by comparing the performance of multiple-resistant phenotypes to those phenotypes that were only resistant to atrazine (S1 and S2). The R1 and R2 phenotypes were consistently shorter and produced less dry matter than the S1 and S2 phenotypes. The R1 and R2 phenotypes were shown to have lower relative growth rates (RGR) and net assimilation rates (NAR) than the S1 and S2 phenotypes at an early stage of growth. However, there was no significant difference in RGR between the R1 and R2 and, S1 and S2 phenotypes at a later stage of growth, though the R1 and R2 phenotypes still had a lower NAR at this later stage. Further investigations using a neighbouring crop competition approach showed that the R1 and R2 phenotypes were weaker competitors, and exhibited significantly less capacity for vegetative growth compared to the S1 and S2 phenotypes during competition. Overall, the results of this study revealed multiple- resistance to atrazine and dicamba endowed a significant fitness penalty to C. album, and it is possible that the frequency of multiple-resistant individuals would gradually decline once selection pressure from herbicides was discontinued.
Subject(s)
Atrazine/pharmacology , Chenopodium album/drug effects , Dicamba/pharmacology , Herbicide Resistance , Herbicides/pharmacology , Biomass , Chenopodium album/growth & development , Chenopodium album/metabolism , Plant Leaves/drug effects , Plant Leaves/growth & development , Plant Leaves/metabolismABSTRACT
The aim of the present study was to obtain an oil/water (O/W) nanoemulsion (NE) containing garden savory (Satureja hortensis) essential oil (EO) and evaluating its herbicidal activity against Amaranthus retroflexus and Chenopodium album. Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the EO. Carvacrol (55.6%) and γ-terpinene (31.9%) were the major EO components. Low energy method was applied, allowing achievement of EO nanodroplets. The NE also presented low polydispersity, and the mean droplet was below 130nm even after storage for 30d. Laboratory tests showed that the NE at different concentrations (100, 200, 400, 800, and 1000µL.L-1) significantly (P≤0.05) reduced the germination indices and the seedling's growth in dose-response. The inhibitory effect was the greatest at 800µL.L-1 NE. Overall, root length was more inhibited as compared to shoot length. Post-emergence application of NE at different concentrations (1000, 2000, 3000, 4000 and 5000µL.L-1 of EO) on 2-4 true leaves' stage of the weeds caused significant (P≤0.05) decrease in the growth factors in dose-dependent manner. Complete lethality was observed by 4000µL.L-1 NE sprayed on the weeds. Spraying of NE significantly (P≤0.05) reduced chlorophyll content in the tested weeds. Increasing in relative electrolyte leakage (REL) 1 and 5d after treatment represented significant cell membrane disruption and increased cell membrane permeability. Transmission electron microscope (TEM) pictures confirmed NE droplet size and demonstrated membrane destruction. The study approved that the NE of S. hortensis EO has herbicidal properties as it has high phytotoxic effect, and interferes with the germination, growth and physiological processes of the weeds. The production of NE from S. hortensis EO is a low energy method that offers a promising practical natural herbicide for weed control in organic agricultural systems.
Subject(s)
Amaranthus/drug effects , Chenopodium album/drug effects , Herbicides/pharmacology , Oils, Volatile/pharmacology , Satureja/chemistry , Weed Control/methods , Amaranthus/growth & development , Chenopodium album/growth & development , Cyclohexane Monoterpenes , Cymenes , Emulsions , Gas Chromatography-Mass Spectrometry , Germination/drug effects , Herbicides/chemistry , Monoterpenes/analysis , Oils, Volatile/chemistry , Particle Size , Seeds/drug effectsABSTRACT
The chemical composition of the essential oils isolated by hydrodistillation from the fruits of four selected Myrtus communis L. genotypes from Turkey was characterized by GC-FID and GC/MS analyses. 1,8-Cineole (29.20-31.40%), linalool (15.67-19.13%), α-terpineol (8.40-18.43%), α-pinene (6.04-20.71%), and geranyl acetate (3.98-7.54%) were found to be the major constituents of the fruit essential oils of all M. communis genotypes investigated. The oils were characterized by high amounts of oxygenated monoterpenes, representing 73.02-83.83% of the total oil compositions. The results of the fungal growth inhibition assays showed that the oils inhibited the growth of 19 phytopathogenic fungi. However, their antifungal activity was generally lower than that of the commercial pesticide benomyl. The herbicidal effects of the oils on the seed germination and seedling growth of Amaranthus retroflexus L., Chenopodium album L., Cirsium arvense (L.) Scop., Lactuca serriola L., and Rumex crispus L. were also determined. The oils completely or partly inhibited the seed germinations and seedling growths of the plants. The findings of the present study suggest that the M. communis essential oils might have potential to be used as natural herbicides as well as fungicides.
Subject(s)
Antifungal Agents/pharmacology , Fruit/chemistry , Fungi/drug effects , Herbicides/pharmacology , Myrtus/chemistry , Myrtus/genetics , Oils, Volatile/pharmacology , Seeds/drug effects , Amaranthus/drug effects , Amaranthus/growth & development , Antifungal Agents/chemistry , Antifungal Agents/isolation & purification , Chenopodium album/drug effects , Chenopodium album/growth & development , Cirsium/drug effects , Cirsium/growth & development , Genotype , Herbicides/chemistry , Herbicides/isolation & purification , Lactuca/drug effects , Lactuca/growth & development , Molecular Structure , Oils, Volatile/chemistry , Oils, Volatile/isolation & purification , Rumex/drug effects , Rumex/growth & development , Seeds/growth & developmentABSTRACT
This review deals with the isolation and chemical and biological characterization of phytotoxins produced by Ascochyta caulina and Phoma chenopodiicola proposed as mycoherbicides for the biological control of Chenopodium album, a worldwide spread weed which causes serious problems to some agrarian crops, including sugar beet and maize. Studies on the structure activity relationships and on the modes of actions of toxins isolated are also described, as well as the optimization of analytical methods focused on selection of the best fungal toxin producers. The attempts to scale up production of these phytotoxins aimed to obtain sufficient amounts for their application in greenhouse and field trials are also reported.
Subject(s)
Ascomycota/chemistry , Chenopodium album/drug effects , Herbicides/toxicity , Mycotoxins/toxicity , Plant Weeds/drug effects , Ascomycota/metabolism , Chenopodium album/growth & development , Herbicides/chemistry , Herbicides/metabolism , Molecular Structure , Mycotoxins/chemistry , Mycotoxins/metabolism , Plant Weeds/growth & development , Weed ControlABSTRACT
It is necessary to elucidate its growth mechanism in order to prevent and control the further spread of Flaveria bidentis, an invasive plant in China. The effects of shading (shading rate of 0, 50% and 80%, respectively) and planting pattern (single cropping of F. bidentis, single cropping of Chenopodium album and their intercropping) on germination rate, fluorescence characteristics and growth characteristics of the two plants were investigated. The results showed that moderate shading contributed to emergence rate, but emergence rate of F. bidentis was not uniform, which was one of important factors as a stronger invader. With the increasing light intensity, net photosynthetic rate (Pn), photochemical quenching (qP), electron transport rate of PS II (ETR), quantum yield of PS II (Y), non-photochemical quenching (qN), water use efficiency (WUE), shoot bio-mass rate (SMR), crown width (CW) and dry biomass (DM) increased, specific leaf area (SLA) decreased, LMR of F. bidentis significantly increased, LMR of C. album changed insignificantly, and the increment of DM of F. bidentis was higher than that of C. album. In 80% shade treatment, Pn and DM of F. bidentis were lower than those of C. album. In natural light treatment, Pn, qN, WUE and relative competitive index (RCI) were the highest, CW and DM of intercropped F. bidentis and Pn, Y of C. album were significantly lower than that of the respective single treatment. F. bidentis had higher light saturation point (LSP) and light compensation point (LCP). In conclusion, the shade-tolerant ability of F. bidentis was weaker than that of C. album, but it was reversed in natural light treatment. The two plants adapted to the weak light in 80% shade treatment by increasing SLA and decreasing LMR. F. bidentis improved competition under natural light by increasing SMR and decreasing CW.
Subject(s)
Chenopodium album/growth & development , Flaveria/physiology , Light , Photosynthesis , Biomass , China , Fluorescence , Introduced Species , Plant LeavesABSTRACT
Plant-soil feedback (PSF) and plant competition play an important role in structuring vegetation composition, but their interaction remains unclear. Recent studies suggest that competing plants could dilute pathogenic effects, whereas the standing view is that competition may increase the sensitivity of the focal plant to PSF. In agro-ecosystems each of these two options would yield contrasting outcomes: reduced versus enhanced effects of weeds on crop biomass production. To test the effect of competition on sensitivity to PSF, we grew Triticum aestivum (Common wheat) with and without competition from a weed community composed of Vicia villosa, Chenopodium album and Myosotis arvensis. Plants were grown in sterilized soil, with or without living field inoculum from 4 farms in the UK. In the conditioning phase, field inocula had both positive and negative effects on T. aestivum shoot biomass, depending on farm. In the feedback phase the differences between shoot biomass in T. aestivum monoculture on non-inoculated and inoculated soils had mostly disappeared. However, T. aestivum plants growing in mixtures in the feedback phase were larger on non-inoculated soil than on inoculated soil. Hence, T. aestivum was more sensitive to competition when the field soil biota was present. This was supported by the statistically significant negative correlation between shoot biomass of weeds and T. aestivum, which was absent on sterilized soil. In conclusion, competition in cereal crop-weed systems appears to increase cereal crop sensitivity to soil biota.
Subject(s)
Ecosystem , Plant Weeds/growth & development , Soil Microbiology , Triticum/growth & development , Agriculture , Biomass , Chenopodium album/growth & development , Linear Models , United Kingdom , Vicia/growth & developmentABSTRACT
In crowded stands, height is often similar among dominant plants, as plants adjust their height to that of their neighbours (height convergence). We investigated which of the factors, light quality, light quantity and mechanical stimuli, is primarily responsible for stem elongation and height convergence in crowded stands. We established stands of potted Chenopodium album plants. In one stand, target plants were surrounded by artificial plants that were painted black to ensure that the light quality was not modified by their neighbours. In a second stand, target plants were surrounded by real plants. In both stands, one-half of the target plants were anchored to stakes to prevent flexing by wind. The target plants were lifted or lowered by 10 cm to test whether height convergence was affected by the different treatments. Stem length was affected by being surrounded by artificial plants, anchoring and pot elevation, indicating that light quality, light quantity and mechanical stimuli all influenced stem elongation. Height convergence did not occur in the stand with artificial plants or in anchored plants. We conclude that light quality and mechanical stimuli are important factors for the regulation of stem growth and height convergence in crowded stands.
Subject(s)
Chenopodium album/anatomy & histology , Chenopodium album/radiation effects , Environment , Light , Plant Stems/growth & development , Plant Stems/radiation effects , Analysis of Variance , Biomass , Biomechanical Phenomena/radiation effects , Chenopodium album/growth & development , Plant Stems/anatomy & histologyABSTRACT
Lead (Pb) has great toxicity to human beings and other livings. Although there are varied ways to rehabilitate the Pb contaminated area, phytoremediation of Pb pollution in arid lands is still a difficult task, it is therefore urgent to find and identify Pb tolerant plants in arid areas. The physiological responses and tolerance mechanisms to Pb stress (expressed as the Pb concentration, e.g., 0, 50, 150, 300, 600, 800, 1000 mg/L) were investigated for the xerophils Salsola passerina Bunge and Chenopodium album L. Results indicated that S. passerina exhibited higher Pb tolerance than Ch. album in terms of the seed germination rate, bio-activities of SOD and POD, and lower MDA production. There were two ways for S. passerina to reduce Pb toxicity in organism level, e.g., cell wall precipitation and state transfer of free Pb into anchorage. These findings demonstrate that S. passerina is a Pb tolerant species and may have potential application in phytoremediation of Pb contaminated arid lands.
Subject(s)
Chenopodium album/drug effects , Lead/toxicity , Salsola/drug effects , Soil Pollutants/toxicity , Biodegradation, Environmental , Chenopodium album/growth & development , Chenopodium album/metabolism , Germination/drug effects , Lead/metabolism , Malondialdehyde/metabolism , Peroxidase/metabolism , Photosynthesis/drug effects , Plant Leaves/drug effects , Plant Leaves/growth & development , Plant Leaves/metabolism , Plant Proteins/metabolism , Plant Roots/drug effects , Plant Roots/growth & development , Plant Roots/metabolism , Plant Stems/drug effects , Plant Stems/growth & development , Plant Stems/metabolism , Salsola/growth & development , Salsola/metabolism , Seedlings/drug effects , Seedlings/growth & development , Seeds/drug effects , Seeds/growth & development , Soil Pollutants/metabolism , Superoxide Dismutase/metabolismABSTRACT
BACKGROUND AND AIMS: Although being tall is advantageous in light competition, plant height growth is often similar among dominant plants in crowded stands (height convergence). Previous theoretical studies have suggested that plants should not overtop neighbours because greater allocation to supporting tissues is necessary in taller plants, which in turn lowers leaf mass fraction and thus carbon gain. However, this model assumes that a competitor has the same potential of height growth as their neighbours, which does not necessarily account for the fact that height convergence occurs even among individuals with various biomass. METHODS: Stands of individually potted plants of Chenopodium album were established, where target plants were lifted to overtop neighbours or lowered to be overtopped. Lifted plants were expected to keep overtopping because they intercept more light without increased allocation to stems, or to regulate their height to similar levels of neighbours, saving biomass allocation to the supporting organ. Lowered plants were expected to be suppressed due to the low light availability or to increase height growth so as to have similar height to the neighbours. KEY RESULTS: Lifted plants reduced height growth in spite of the fact that they received higher irradiance than others. Lowered plants, on the other hand, increased the rate of stem elongation despite the reduced irradiance. Consequently, lifted and lowered plants converged to the same height. In contrast to the expectation, lifted plants did not increase allocation to leaf mass despite the decreased stem length. Rather, they allocated more biomass to roots, which might contribute to improvement of mechanical stability or water status. It is suggested that decreased leaf mass fraction is not the sole cost of overtopping neighbours. Wind blowing, which may enhance transpiration and drag force, might constrain growth of overtopping plants. CONCLUSIONS: The results show that plants in crowded stands regulate their height growth to maintain similar height to neighbours even when they have potential advantages in height growth. This might contribute to avoidance of stresses caused by wind blowing.
Subject(s)
Chenopodium album/growth & development , Light , Plant Stems/growth & development , Biomass , Biometry , Chenopodium album/radiation effects , Japan , Models, Biological , Plant Stems/radiation effects , Stress, Physiological , Water/metabolism , WindABSTRACT
Molecular markers can provide valuable information on the spread of resistant weed biotypes. In particular, tracing local spread of resistant weed patches will give details on the importance of seed migration with machinery, manure, wind or birds. This study investigated the local spread of metamitron resistant Chenopodium album L. patches in the southwest region of the province West-Flanders (Belgium). During the summer of 2009, leaf and seed samples were harvested in 27 patches, distributed over 10 sugar beet fields and 1 maize field. The fields were grouped in four local clusters. Each cluster corresponded with the farmer who cultivated these fields. A cleaved amplified polymorphic sequence (CAPS) procedure identified the Ser264 to Gly mutation in the D1 protein, endowing resistance to metamitron, a key herbicide applied in sugar beet. The majority of the sampled plants within a patch (97% on average) carried this mutation. Amplified fragment length polymorphism (AFLP) analysis was performed with 4 primer pairs and yielded 270 molecular markers, polymorphic for the whole dataset (303 samples). Analysis of molecular variance revealed that a significant part of the genetic variability was attributed to variation among the four farmer locations (12 %) and variation among Chenopodium album patches within the farmer locations (14%). In addition, Mantel tests revealed a positive correlation between genetic distances (linearised phipt between pairs of patches) and geographic distances (Mantel-coefficient significant at p = 0.002), suggesting isolation-by-distance. In one field, a decreased genetic diversity and strong genetic relationships between all the patches in this field supported the hypothesis of a recent introduction of resistant biotypes. Furthermore, genetic similarity between patches from different fields from the same farmer and from different farmers indicated that seed transport between neighbouring fields is likely to have an important impact on the spread of metamitron resistant biotypes.
Subject(s)
Chenopodium album/growth & development , Herbicide Resistance , Herbicides/pharmacology , Plant Weeds/growth & development , Triazines/pharmacology , Amplified Fragment Length Polymorphism Analysis , Belgium , Chenopodium album/classification , Chenopodium album/drug effects , Chenopodium album/genetics , Genetic Variation , Mutation , Phylogeny , Plant Proteins/genetics , Plant Weeds/classification , Plant Weeds/drug effects , Plant Weeds/geneticsABSTRACT
Plants are rich source of biologically active allelochemicals. However, natural product discovery is not an easy task. Many problems encountered during this laborious practice can be overcome through the modification of preliminary trials. Bioassay-directed isolation of active plant compounds can increase efficiency by eliminating many of the problems encountered. This strategy avoids unnecessary compounds, concentrating on potential components and thus reducing the cost and time required. In this study, a crude aqueous extract of sunflower leaves was fractionated through high performance liquid chromatography. The isolated fractions were checked against Chenopodium album and Rumex dentatus. The fraction found active against two selected weeds was re-fractionated, and the active components were checked for their composition. Thin layer chromatography isolated a range of phenolics, whereas the presence of bioactive terpenoids was confirmed through mass spectroscopy and nuclear magnetic resonance spectroscopy.
Subject(s)
Helianthus/chemistry , Phenols/isolation & purification , Phenols/pharmacology , Pheromones/isolation & purification , Pheromones/pharmacology , Biomass , Chenopodium album/drug effects , Chenopodium album/growth & development , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Magnetic Resonance Spectroscopy , Mass Spectrometry , Rumex/drug effects , Rumex/growth & development , WaterABSTRACT
If growing cells in plants are considered to be composed of increments (ICs) an extended version of the law of mass action can be formulated. It evidences that growth of plants runs optimal if the reaction-entropy term (entropy times the absolute temperature) matches the contact energy of ICs. Since these energies are small, thermal molecular movements facilitate via relaxation the removal of structure disturbances. Stem diameter distributions exhibit extra fluctuations likely to be caused by permanent constraints. Since the signal-response system enables in principle perfect optimization only within finite-sized cell ensembles, plants comprising relatively large cell numbers form a network of size-limited subsystems. The maximal number of these constituents depends both on genetic and environmental factors. Accounting for logistical structure-dynamics interrelations, equations can be formulated to describe the bimodal growth curves of very different plants. The reproduction of the S-bended growth curves verifies that the relaxation modes with a broad structure-controlled distribution freeze successively until finally growth is fully blocked thus bringing about "continuous solidification".
Subject(s)
Plant Development , Chenopodium album/growth & development , Entropy , Fagus/growth & development , Models, Biological , Plant Stems/growth & development , TemperatureABSTRACT
Chenopodium album L. (fat-hen) with a Ser264-Gly mutation is resistant to photosystem II-inhibiting herbicides like the triazinone metamitron, a key herbicide in sugar beet. In recent years, this resistant biotype may cause unsatisfactory weed control in Belgian sugar beet. However, the dimension of the problem was yet unknown. Therefore, a survey was conducted in 2008 covering the whole Belgian sugar beet area. In randomly selected fields, C. album plants surviving weed control were counted and sampled. First, the number of surviving plants was used to estimate the prevalence of fields with unsatisfactory control and to classify the surveyed fields. Then, the share of the resistant biotype in each field was determined with cleaved amplified polymorphic sequence-analysis (CAPS-analysis) on sampled leaves. Finally, all results were visualised on the map of Belgium. Twenty percent of the fields had more than 500 surviving plants per hectare and were thus classified as fields with unsatisfactory C. album control. The resistant biotype was present in 95% of these fields and even in 74% of the sampled fields with good weed control. No pattern was found during mapping. These results indicate that the metamitron-resistant biotype has spread over the whole sugar beet area but that it is not (yet) causing severe problems in every field. To get a more accurate estimation of the portion of resistant plants in the field and the effect of herbicide treatment on this biotype, an elaborate survey will be conducted in 2010 on fields that have both untreated and treated plots installed.
Subject(s)
Chenopodium album/genetics , Weed Control , Belgium , Beta vulgaris/growth & development , Chenopodium album/growth & development , Chenopodium album/toxicity , Genotype , Herbicide Resistance , Herbicides/toxicity , Triazines/toxicityABSTRACT
The composition of weed species in spring barley and weed biodiversity was evaluated in experiments in different growing seasons and with different previous crops. The aim of the experiments was to evaluate the composition of weed species in spring barley during a four year period in weather conditions of different growing seasons and with different previous crop as well as to assess the biodiversity in the experiments where the different groups of herbicides were applied. Over years and previous crops, the dicotyledonous weed community was dominated by Chenopodium album, followed by Viola arvensis. The herbicides from different groups had significant influences on the biodiversity of weeds.
Subject(s)
Biodiversity , Hordeum/classification , Plant Weeds/classification , Chenopodium album/growth & development , Herbicides/toxicity , Hordeum/growth & development , Latvia , Plant Weeds/growth & development , Seasons , Viola/growth & development , Weather , Weed Control/methodsABSTRACT
A central paradigm of life-history theory is the existence of resource mediated trade-offs among different traits that contribute to fitness, yet observations inconsistent with this tenet are not uncommon. We previously found a clonal population of the aphid Myzus persicae to exhibit positive genetic correlations among major components of fitness, resulting in strong heritable fitness differences on a common host. This raises the question of how this genetic variation is maintained. One hypothesis states that variation for resource acquisition on different hosts may override variation for allocation, predicting strong fitness differences within hosts as a rule, but changes in fitness hierarchies across hosts due to trade-offs. Therefore, we carried out a life-table experiment with 17 clones of M. persicae, reared on three unrelated host plants: radish, common lambsquarters and black nightshade. We estimated the broad-sense heritabilities of six life-history traits on each host, the genetic correlations among traits within hosts, and the genetic correlations among traits on different hosts (cross-environment genetic correlations). The three plants represented radically different environments with strong effects on performance of M. persicae, yet we detected little evidence for trade-offs. Fitness components were positively correlated within hosts but also between the two more benign hosts (radish and lambsquarters), as well as between those and another host tested earlier. The comparison with the most stressful host, nightshade, was hampered by low survival. Survival on nightshade also exhibited genetic variation but was unrelated to fitness on other hosts. Acknowledging that the number of environments was necessarily limited in a quantitative genetic experiment, we suggest that the rather consistent fitness hierarchies across very different plants provided little evidence to support the idea that the clonal variation for life-history traits and their covariance structure are maintained by strong genotypexenvironment interactions with respect to hosts. Alternative explanations are discussed.
Subject(s)
Aphids/genetics , Genetic Variation , Plant Development , Animals , Aphids/anatomy & histology , Aphids/physiology , Biological Evolution , Body Size , Chenopodium album/growth & development , Fertility , Genotype , Raphanus/growth & development , Solanum/growth & developmentABSTRACT
Chenopodium album L. (fat-hen), a highly competitive and very prolific species, is a common weed in most spring- and summer-sown crops such as maize, sugar beet and vegetables. In the late seventies, C. album stepped into the limelight as a problem weed in maize. Frequent use of atrazine in maize monoculture did select for plants having a Ser-264-Gly mutation on the psbA gene resulting in atrazine-resistance and cross-resistance to other Photosystem (PS) II-inhibitors. The psbA gene encodes the D1 protein of PS II which is the target site of PS II-inhibitors. Introduction of new herbicides made it possible to control this atrazine-resistant biotype in maize, which allowed C. album to fade into the background again until it resurfaced some years ago as a problem weed in European sugar beet (Belgium, France, The Netherlands and Sweden). Greenhouse bioassays at Ghent University revealed that the unsatisfactory control of C. album in sugar beet is due to resistance to the triazinone metamitron, a key herbicide in sugar beet. The expected cross-resistance to atrazine and metribuzin was found in all populations except for a Swedish one, which is highly resistant to metamitron and metribuzin but not to atrazine. DNA sequence analysis confirmed the presence of a Ser-264-Gly mutation for all populations that are both metamitron- and atrazine-resistant. The Swedish population has an Ala-251-Val mutation on the psbA gene explaining its aberrant (cross)-resistance profile. The occurrence of C. album biotypes with resistance to metamitron but different genotypes and cross-resistance profiles could raise the question which herbicide(s) did select for the resistance. In Sweden, having no history of atrazine use, the triazinones metamitron, used in sugar beet, and metribuzin, used in rotational potato, could have selected for resistance. In Belgium, at least three different herbicides and/or crop rotations could have contributed to resistance development: (1) a record of continuous use of atrazine in maize resulting in triazine-resistant C. album in the seed bank, (2) metamitron use in sugar beet and (3) metribuzin use in potato.
Subject(s)
Beta vulgaris/growth & development , Chenopodium album/drug effects , Herbicide Resistance/genetics , Herbicides/pharmacology , Pest Control/methods , Zea mays/growth & development , Atrazine/pharmacology , Chenopodium album/genetics , Chenopodium album/growth & development , Dose-Response Relationship, Drug , Genotype , Triazines/pharmacologyABSTRACT
This paper presents the results of laboratory tests on a selection of weeds (Viola arvensis, Polygonum persicaria, Chamomilla recutita, Chenopodium album, Veronica persicaria, Alopecurus myosusroides) to investigate the efficiency of flupyrsulfuron-methyl plus metsutfuronmethyl (Lexus XPE) in combination with different adjuvants. The efficiency of the herbicide improved in combination of adjuvants. The level of phytotoxicity of the adjuvants-herbicide treatments appllied varied among the different weed species.
Subject(s)
Arylsulfonates/pharmacology , Pesticide Synergists/pharmacology , Photolysis , Poaceae/drug effects , Pyrimidines/pharmacology , Sulfonylurea Compounds/pharmacology , Adjuvants, Pharmaceutic/pharmacology , Chenopodium album/drug effects , Chenopodium album/growth & development , Dose-Response Relationship, Drug , Herbicides , Matricaria/drug effects , Matricaria/growth & development , Poaceae/growth & development , Polygonum/drug effects , Polygonum/growth & development , Veronica/drug effects , Veronica/growth & development , Viola/drug effects , Viola/growth & developmentABSTRACT
This study shows the ability of Artificial Neural Network (ANN) technology to be used for the prediction of the correlation between common lambsquarters (Chenopodium album L.) population, corn (Zea mays L.) population and planting pattern in different days after planting (as inputs) with common lambsquarters biomass production (as output). The number of patterns used in this study was 60 which were randomly divided into 45 and 15 data sets for training and testing the neural network, respectively. The results showed that a very good performance of the neural network is achieved. Some explanation of the predicted results is given. The multi layer perceptrons with training algorithm of backpropagation (BP) was the best one for creating nonlinear mapping between input and output parameters. The mean training of root mean square error (RMSE) was equal to 0.0156. ANN model predicted the common lambsquarters biomass with maximum RMSE, t-value, average prediction error and correlation coefficient of 0.0091, 0.985, 2.6% and 0.989, respectively. The ANN model, predicted common lambsquarters biomass within +/- 5% of the measured biomass for 59.8% of the samples indicates that the ANN can potentially be used to estimate plant biomass. Adjusting ANN parameters such as learning rate, momentum, number of patterns and number of hidden nodes/layers affected the accuracy of biomass production predictions.
Subject(s)
Agriculture/methods , Chenopodium album/growth & development , Models, Biological , Neural Networks, Computer , Zea mays/growth & development , Algorithms , Biomass , Computer SimulationABSTRACT
The metal accumulation potential of Chenopodium album L. grown on various amendments of tannery sludge (TS) was studied after 60 days of sapling planted. The analysis of the results showed that the levels of pH, cation exchange capacity, organic carbon, organic matter and DTPA extractable metals (except Mn) of amendments increased by the addition of tannery sludge ratio. Shoot length of the plant increased by the addition of sludge, whereas, no marked change was observed in root length, fresh and dry weight of the plant. Accumulation of the metals in the plants was found in the order; Fe > Mn > Zn > Cr > Cu > Pb > Ni > Cd. Translocation of toxic metals (Cr, Pb, Cd) in different parts of the tested plant was found in the order; leaves > stems > roots. An increase in the photosynthetic pigments, carotenoid and leaf protein contents of the plants were found to increase with increase in sludge amendments. Correlation analysis between metal accumulation in the plants with DTPA extractable metals emphasized that Mn, Ni, Cr, Pb and Cd showed positive correlation (p < 0.05), whereas, Fe, Zn and Cu showed negative correlation. Transfer factor analysis emphasized that 10% TS amendments were suitable for phytoextraction of Cr. Overall analysis of the data exhibited that the plants may be used for phytoextraction of Cr from tannery waste contaminated soil as most of the metal was accumulated in harvestable part which is a matter of serious concern, whenever used for edible purposes.