ABSTRACT
In the quest for novel tools for early detection and treatment of cancer, we propose the use of multimers targeting overexpressed receptors at the cancer cell surface. Indeed, multimers are prone to create multivalent interactions, more potent and specific than their corresponding monovalent versions, thus enabling the potential for early detection. There is a lack of tools for early detection of pancreatic cancer, one of the deadliest forms of cancer, but CCK2-R overexpression on pancreatic cancer cells makes CCK based multimers potential markers for these cells. In this Letter, we describe the synthesis and evaluation of CCK trimers targeting overexpressed CCK2-R.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Pancreatic Neoplasms/metabolism , Biomarkers, Tumor/biosynthesis , Biomarkers, Tumor/chemistry , Cholecystokinin/chemistry , Humans , Pancreatic Neoplasms/diagnosis , Pancreatic Neoplasms/drug therapy , Receptors, Cholecystokinin/biosynthesis , Receptors, Cholecystokinin/chemistry , Receptors, Cholecystokinin/metabolismABSTRACT
Two gemini surfactants, [C18CysL5CCK8](2) and [C18CysDTPAGlu](2), containing, respectively, the CCK8 peptide and the DTPAGlu chelating agent or its gadolinium complex have been prepared by linking lipophilic chains through a disulfide bond between two cysteine residues. The two surfactants aggregate in water solution forming pure or mixed micelles, with a critical micellar concentration in the 5 x 10(-6)-5 x 10(-5) mol kg(-1) range, as measured by fluorescence spectroscopy. As indicated by small-angle neutron scattering, the shape and size of the micelles are influenced by the temperature: increasing temperature leads to progressive reduction of the size of the supramolecular aggregates. Cylindrical structures found at lower temperatures (10-40 degrees C) evolve into ellipsoidal micelles at 50-80 degrees C. Furthermore, the surface-exposed CCK8 peptide changes its conformation above a transition temperature of approximately 45 degrees C, going from a beta-sheet to a random-coil structure, as indicated by circular dichroism measurements. The mixed aggregate obtained by coaggregation of the two gemini-based amphiphilic compounds, [C18CysDTPAGlu(Gd)](2) and [C18CysL5CCK8](2) in 70:30 molar ratio, represents the first example of a peptide-containing gemini surfactant as a potential target-selective contrast agent in MRI. In fact, it presents a high relaxivity value of the gadolinium complex, 21.5 mM(-1) s(-1), and the CCK8 bioactive peptide exposed on the external surface is therefore capable of selective targeting of the cholecystokinin receptors.
Subject(s)
Cholecystokinin/chemistry , Cholecystokinin/metabolism , Gadolinium/chemistry , Micelles , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Protein Conformation , Surface-Active Agents/chemistry , Cholecystokinin/chemical synthesis , Cholecystokinin/genetics , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Peptide Fragments/chemical synthesis , Peptide Fragments/genetics , Surface-Active Agents/chemical synthesisABSTRACT
The development of receptor targeting radiolabeled ligands has gained much interest in recent years for diagnostic and therapeutic applications in nuclear medicine. Cholecystokinin (CCK) receptors have been shown to be overexpressed in a subset of neuroendocrine and other tumors. We are evaluating binding and biodistribution properties of a CCK8 peptide derivative labeled with (99m)Tc(I)-tricarbonyl. The CCK8 peptide was modified at its N-terminus by adding to its N-terminus two lysine-histidine modules (KH), where histidine is coupled to the side chain of the lysine ((KH)(2)-CCK8). (99m)Tc(I)-tricarbonyl was generated with the IsoLinktrade mark kit. A431 cells stably transfected with a cDNA encoding for the human CCK2 receptor were utilized to determine binding affinity, internalization, and retention of the labeled peptide, in comparison with wild-type A431 cells. A nude mouse tumor model was obtained by generating A431-CCK2R and A431-control tumors in opposite flanks of the animals. High specific activity labeling with (99m)Tc was achieved. In A431-CCK2R cells, specific saturable binding was observed as well as evident internalization of the radiolabeled peptide after binding. Biodistribution experiments showed rapid, specific localization of (KH)(2)-CCK8 on A431-CCK2R xenografts compared with control tumors, although absolute uptake values were not markedly higher compared with background activity. Clearance of unbound radioactivity was both urinary and hepatobiliary. In imaging experiments, while targeting to CCK2R positive tumors could be appreciated, there was poor contrast between target and nontarget areas. (KH)(2)-CCK8 shows adequate in vitro and in vivo properties for CCK2R targeting although improvement of biodistribution warrant further development.
Subject(s)
Chelating Agents/chemistry , Cholecystokinin/chemical synthesis , Histidine/analogs & derivatives , Histidine/chemistry , Organotechnetium Compounds/metabolism , Peptide Fragments/chemical synthesis , Radiopharmaceuticals , Animals , Cell Line, Tumor , Cholecystokinin/metabolism , Drug Design , Histidine/metabolism , Humans , Isotope Labeling , Mice , Mice, Nude , Neoplasm Transplantation , Peptide Fragments/metabolism , Receptors, Cholecystokinin/metabolism , Transplantation, HeterologousABSTRACT
A library of 14 cyclic peptide analogues derived from the octapeptide C-terminal sequence of the human cholecystokinin hormone (CCK(26-33), or CCK8) was designed, synthesized, and characterized. The 14 peptide analogues were rationally designed to specifically interact with the CCK type B receptor (CCK(B)-R) on the basis of the structure of the bimolecular complex between CCK8 and the third extracellular loop of CCK(B)-R, namely CCK(B)-R(352-379). The rational design of new ligands for CCK(B)-R has relied on stabilization by cyclic constraints of the structural motifs that bring the key residues of the ligand (especially Trp 30, Met 31, and Phe 33) in the proper spatial orientation for optimal interaction with the receptor. The binding affinity of the new ligands for CCK(B)-R was assessed by displacement experiments of (111)In-radiolabeled CCK8 in cells that overexpress the CCK(B) receptor. The new ligands generally showed binding affinities lower than that of parent CCK8, with the best compounds having IC50 values around 10 microM. Structure-activity relationship data show that preservation of the Trp 30-Met 31 motif is essential and that the Phe 33 side chain must be present. NMR conformational studies of the compound with maximal binding affinity (cyclo-B11, IC50=11 microM) in DPC micelles shows that this compound presents a turn-like conformation centered at the Trp 30-Met 31 segment, as planned by rational design. Such a conformation is stabilized by its interaction with the micelle rather than by the cyclic constraint.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemistry , Receptors, Cholecystokinin/antagonists & inhibitors , Cholecystokinin/chemical synthesis , Drug Design , Ligands , Magnetic Resonance Spectroscopy , Models, Molecular , Molecular Conformation , Peptide Library , Peptides/chemical synthesis , Peptides/chemistryABSTRACT
In this work, we 1) synthesized rat CCK-58, 2) determined the amounts and forms of rat CCK in whole blood after stimulation of its release by casein, 3) determined the potency of CCK-8 and CCK-58 peptides to displace labeled CCK-8 from CCK(A) and CCK(B) receptors transfected into Chinese hamster ovary (CHO) cells, and 4) examined the biological actions of CCK-8 and rat CCK-58 in an anesthetized rat model. CCK-58 was the only detected endocrine form of CCK in rat blood. Synthetic rat CCK-58 was less potent than CCK-8 for displacing the label from CCK(A) and CCK(B) receptors in transfected CHO cells. However, rat CCK-58 was more potent than CCK-8 for stimulation of pancreatic protein secretion in the anesthetized rat. In addition, CCK-58 but not CCK-8 stimulated fluid secretion in this anesthetized rat model. These data suggest that regions outside the COOH terminus of rat CCK-58 influence the expression of CCK biological activity. The presence of only CCK-58 in the circulation and the fact that its biological activity differs from CCK-8 suggests that CCK-58 deserves scrutiny in other physiological models of CCK activity.
Subject(s)
Bile/metabolism , Cholecystokinin/pharmacology , Pancreas/metabolism , Sincalide/pharmacology , Amino Acids/analysis , Amylases/metabolism , Anesthesia , Animals , Bile/drug effects , Binding, Competitive/drug effects , CHO Cells , Caseins/pharmacology , Cholecystokinin/blood , Cholecystokinin/chemical synthesis , Cricetinae , Mass Spectrometry , Pancreas/drug effects , Radioimmunoassay , Rats , Rats, Sprague-Dawley , Receptor, Cholecystokinin A/drug effects , Receptor, Cholecystokinin A/metabolism , Receptor, Cholecystokinin B/drug effects , Receptor, Cholecystokinin B/metabolism , Sincalide/blood , Spectrometry, Mass, Fast Atom Bombardment , Sulfates/metabolismABSTRACT
[(3)H]BBL454, a new CCK(2) selective tritiated agonist was prepared via the reductive tritiation of a 5-aminopentyn-1-yl moiety introduced on the N-terminal end of a pentapeptide derivative of cholecystokinin. The binding properties of this labelled compound were determined on CHO cells transfected with the rat CCK(2) receptor. [(3)H]BBL454 is able to discriminate two affinity states of the CCK(2) receptor a supplementary indication of its validity for further exploring the heterogeneity of this receptor.
Subject(s)
Cholecystokinin/chemical synthesis , Cholecystokinin/metabolism , Oligopeptides/chemical synthesis , Peptide Fragments/chemical synthesis , Peptide Fragments/metabolism , Receptor, Cholecystokinin B/agonists , Animals , CHO Cells , Cholecystokinin/analogs & derivatives , Cricetinae , Guinea Pigs , Oligopeptides/metabolism , Radioligand Assay/methods , Rats , Receptor, Cholecystokinin B/metabolism , Tritium/metabolismABSTRACT
The carboxyl terminal octapeptide of cholecystokinin (CCK-8) has been hypothesized to account for the bioactivity of all the molecular forms of cholecystokinin. However, the physiological relevance of CCK-58 has not been rigorously examined because of the lack of sufficient amounts of the peptide and concerns about inactivation of natural peptides during their purification. Therefore, canine-sulfated CCK-58 was synthesized and conditions determined for its unblocking and purification that preserved the sulfated tyrosine. Synthetic CCK-58 was indistinguishable from natural CCK-58 by amino acid analysis and by mass spectrometry. Synthetic CCK-58 and CCK-8 have different patterns of pancreatic stimulation: both caused a dose-related increase in amylase release, while only CCK-58 stimulated bile-pancreatic output volume. Thus, CCK-58 and CCK-8 are biased agonists at the CCK-A receptor (they have distinct patterns of action mediated by the same receptor). Previous work has demonstrated that the identical carboxyl termini of CCK-8 and CCK-58 have different solution conformations. Taken together, the physiological and structural results support the hypothesis that different carboxyl terminal conformations of CCK-58 and CCK-8 alter the expression of their biological activity.
Subject(s)
Cholecystokinin/chemical synthesis , Cholecystokinin/pharmacology , Amino Acids/metabolism , Amylases/metabolism , Animals , Dogs , Dose-Response Relationship, Drug , Mass Spectrometry/methods , Pancreas/drug effects , Pancreas/metabolism , Peptide Fragments/chemical synthesis , Peptide Fragments/pharmacology , Time FactorsABSTRACT
A novel CCK8 derivative bearing a chelating agent at its N- end and its oxo-rhenium(V) complex have been synthesized and characterized. The chelating agent N-[N-13-(diphenylphosphino)propionyl]glycyl]cysteine (PN2S) ligand, the coordination set of which is made by the phosphorus atom of phosphine, the nitrogen atoms of the two amido groups and the sulphur atom of cysteine, has been used due to its high affinity towards the oxo-rhenium(V) moiety. Molecular modelling studies indicate that the CCK8 peptide adopts the right conformation for cholecystokinin receptor binding, and that modifications on the N-terminal side of CCK8 obtained by introducing chelating agents and its metal complexes should not affect the interaction with CCK(A) receptor.
Subject(s)
Cholecystokinin/chemistry , Cholecystokinin/chemical synthesis , Isotope Labeling/methods , Peptide Fragments/chemistry , Peptide Fragments/chemical synthesis , Phenols/chemistry , Phosphines/chemistry , Rhenium/chemistry , Amino Acid Sequence , Models, Molecular , Molecular Structure , Protein ConformationABSTRACT
Chemical synthesis of tyrosine O-sulfated peptides is still a laborious task for peptide chemists because of the intrinsic acid-lability of the sulfate moiety. An efficient cleavage/deprotection procedure without loss of the sulfate is the critical difficulty remaining to be solved for fluoren-9-ylmethoxycarbonyl (Fmoc)-based solid-phase synthesis of sulfated peptides. To overcome the difficulty, TFA-mediated solvolysis rates of a tyrosine O-sulfate [Tyr(SO3H)] residue and two protecting groups, tBu for the hydroxyl group of Ser and 2,2,4,6,7-pentamethyldihydrobenzofuran-5-sulfonyl (Pbf) for the guanidino group of Arg, were examined in detail. The desulfation obeyed first-order kinetics with a large entropy (59.6 J.K-1.mol-1) and enthalpy (110.5 kJ.mol-1) of activation. These values substantiated that the desulfation rate of the rigidly solvated Tyr(SO3H) residue was strongly temperature-dependent. By contrast, the SN1-type deprotections were less temperature-dependent and proceeded smoothly in TFA of a high ionizing power. Based on the large rate difference between the desulfation and the SN1-type deprotections in cold TFA, an efficient deprotection protocol for the sulfated peptides was developed. Our synthetic strategy for Tyr(SO3H)-containing peptides with this effective deprotection protocol is as follows: (i) a sulfated peptide chain is directly constructed on 2-chlorotrityl resin with Fmoc-based solid-phase chemistry using Fmoc-Tyr(SO3Na)-OH as a building block; (ii) the protected peptide-resin is treated with 90% aqueous TFA at 0 degree C for an appropriate period of time for the cleavage and deprotection. Human cholecystokinin (CCK)-12, mini gastrin-II (14 residues), and little gastrin-II (17 residues) were synthesized with this method in 26-38% yields without any difficulties. This method was further applied to the stepwise synthesis of human big gastrin-II (34 residues), CCK-33 and -39. Despite the prolonged acid treatment (15-18 h at 0 degree C), the ratios of the desulfated peptides were less than 15%, and the pure sulfated peptides were obtained in around 10% yields.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Gastrins/chemical synthesis , Peptides/chemical synthesis , Protein Precursors/chemical synthesis , Tyrosine/chemistry , Amino Acid Sequence , Animals , Chromatography, High Pressure Liquid , Humans , Hydrolysis , In Vitro Techniques , Indicators and Reagents , Islets of Langerhans/drug effects , Islets of Langerhans/metabolism , Kinetics , Male , Molecular Sequence Data , Peptides/chemistry , Rats , Serine Endopeptidases/chemistry , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Sulfates/chemistry , Water/chemistryABSTRACT
New DOTA-based bifunctional prochelators, e.g., 1-(1-carboxy-3-carbotertbutoxypropyl)-4,7,10-(carbotertbutoxyme thyl)-1,4,7,10-tetraazacyclodode-cane (DOTAGA(tBu)4), (6d) for a broad application in the modification of biomolecules with metal ions were prepared. The five-step synthesis of 6d has an overall yield of about 20%. The coupling of 6d to a bioactive peptide on solid-phase was exemplified with use of a CCK-B (cholecystokinin) analogue.
Subject(s)
Chelating Agents/chemical synthesis , Prodrugs/chemical synthesis , Cholecystokinin/chemical synthesis , Molecular Mimicry , Oligopeptides/chemical synthesis , Organometallic Compounds/chemical synthesis , Radioisotopes/chemistrySubject(s)
Cholecystokinin/pharmacology , Conflict, Psychological , Motor Activity/drug effects , Peptide Fragments/pharmacology , Psychotropic Drugs/pharmacology , Animals , Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Learning/drug effects , Pain Measurement , Peptide Fragments/chemical synthesis , Psychotropic Drugs/chemical synthesis , Rats , Receptors, Cholecystokinin/agonistsABSTRACT
A systematic investigation of solid-phase peptide synthesis at elevated temperatures using the well-known aggregating peptide acyl carrier protein (65-74) and the unsulfated cholecystokinin-8 as models is presented. The main goal of the investigation was the determination of an optimized experimental condition for the synthesis of unsulfated cholecystokinin-12. Of the elevated temperatures used, 60 degrees C was the most appropriate. The efficiency of N,N'-diisopropylcarbodiimide/1-hydroxybenzotriazole (DIC/HOBt) in 25% dimethyl sulfoxide (DMSO)/toluene at this temperature was similar to that of 2-(1-H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU). Interestingly, this coupling reagent was more efficient than TBTU, benzotriazol-1-yl oxy-tris(dimethylamino)phosphonium and O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate in N-methylpyrrolidone. 25% DMSO/toluene proved to be suitable for the swelling of the resins phenylacetamidomethyl, methylbenzhydrylamine, hydroxymethylphenoxy, 4-(benzyloxy)-2',4'-dimethoxybenzhydrylamine, 4-(2',4'-dimethoxyphenyl-Fmoc-aminomethyl)phenoxy and (4-succinylamido-2',2',4'-trimethoxy)benzhydrylamine. Those polymeric supports were fully compatible with the approach. Under the optimized synthesis condition found in these studies (temperature of 60 degrees C, DIC/HOBt as coupling reagent and 25% DMSO/toluene as solvent), no difficulties related to the aggregation phenomenon were encountered. These data confirm the usefulness of solid-phase peptide synthesis at elevated temperatures and extend its applicability.
Subject(s)
Cholecystokinin/chemical synthesis , Peptide Fragments/chemical synthesis , Acyl Carrier Protein/chemical synthesis , Chromatography, High Pressure Liquid , Peptide Fragments/isolation & purification , Sincalide/chemical synthesis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Sulfates/chemistry , TemperatureABSTRACT
We describe selective CCKA receptor antagonists, based on the 1-oxo-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid core. Selectivity A vs B is discussed on the basis of molecular modelling. Chemical preparation uses electrophilic cyclization of isocyanates derivating from unnatural tryptophan esters. A stereoselective version of the reaction is given. A few peptides incorporating unnatural tryptophans are prepared, with a view of SAR.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Receptors, Cholecystokinin/antagonists & inhibitorsABSTRACT
The sequence of a cholecystokinin (CCK) related peptide was modified to obtain analogues, which interact selectively either with CCK-B, or with delta-opioid receptors. Two kinds of peptides were designed, namely, the cyclic peptides of the H-Tyr-cyclo (D-Pen-Gly-Trp-L/D-3-transmercaptoproline)-Asp-Phe-NH2 sequence (compounds 1a and 1b, respectively), and the linear peptides of the H-Tyr-D-Val-Gly-Trp-L/D-3-trans-methylmercaptoproline-Asp-Phe- NH2 sequence (compounds 2a and 2b, respectively). The only difference between the chemical structures of the linear analogues compared to the cyclic ones is that one covalent bond has been eliminated and a sulfur atom is replaced by a methyl group. Molecular modeling showed that, among low-energy conformers of cyclic compounds 1, there are three-dimensional structures compatible to the model for delta-receptor-bound conformer, suggested earlier [G. V. Nikiforovich, V.J. Hruby, O. Prakash, and C.A. Gehrig (1991) Biopolymers, vol. 31, pp. 941-955]. Results of binding assays fully supported the rationale for the design of compounds 1 and 2. The cyclic analogue 1a has Ki values of 4.5 and > 5000 nM at delta- and mu-opioid receptors, respectively; and IC50 values of 1.6 and > 10,000 nM for CCK-A and CCK-B receptors, respectively. The results of this study demonstrate a possibility to redirect a peptide sequence that interacts with one type of receptors (CCK-B receptors) toward interaction with another type (delta-opioid receptors) belonging to a different physiological system. This redirection could be performed by changing the conformational properties of the peptide with very minimal changes in its chemical structure.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemistry , Narcotics/chemistry , Oligopeptides/chemistry , Protein Conformation , Receptors, Cholecystokinin/metabolism , Receptors, Opioid, delta/metabolism , Amino Acid Sequence , Animals , Cell Line , Cholecystokinin/chemical synthesis , Cholecystokinin/metabolism , Humans , Kinetics , Ligands , Male , Models, Molecular , Molecular Sequence Data , Narcotics/chemical synthesis , Narcotics/metabolism , Oligopeptides/chemical synthesis , Oligopeptides/metabolism , Pancreas/metabolism , Rats , Rats, Wistar , Structure-Activity RelationshipABSTRACT
This article describes a rationale and strategy for the design of low molecular weight, non-peptide ligands (peptoids), using the chemical structure of mammalian neuropeptides as a starting point. These peptoids may act as either agonists or antagonists at neuropeptide receptors. As they are non-peptides, they can serve as robust tools to help establish the role of the peptides in models of physiological and pathophysiological processes and indeed they may emerge as therapeutic agents in their own right. The strategy is exemplified by the first rational design of 'peptoid' ligands for cholecystokinin (CCK) and tachykinin receptors.
Subject(s)
Neuropeptides , Amino Acid Sequence , Animals , Cholecystokinin/agonists , Cholecystokinin/antagonists & inhibitors , Cholecystokinin/chemical synthesis , Drug Design , Humans , Ligands , Molecular Sequence Data , Neuropeptides/agonists , Neuropeptides/antagonists & inhibitors , Neuropeptides/chemical synthesis , Peptoids , Tachykinins/agonists , Tachykinins/antagonists & inhibitors , Tachykinins/chemical synthesisABSTRACT
Two derivatives of the peptide hormone cholecystokinin (CCK-8s) containing a photocleavable o-nitrobenzylester group have been synthesized. One analog, [3H]4-(biotin-epsilon-Ahx-oxymethyl)-3-nitrobenzoyl-Gly-Orn (propionyl)-epsilon-Ahx-CCK-8s ([3H]BANA-CCK-8s), was biotinylated, while the other, 4-alanyloxymethyl-3-nitrobenzoyl-epsilon-Ahx-CCK-8s (ANA-CCK-8s) had a free amino group for coupling to amino-reactive affinity matrices. The analogs retained high affinity to both CCK receptors and anti-CCK antibodies. [3H]BANA-CCK-8s was bound to a streptavidin-agarose affinity matrix and was subsequently released by irradiation with uv light of wavelengths > 320 nm. The ability to recover a biotinylated peptide ligand from a streptavidin column under very mild conditions should be useful in the purification of specifically binding proteins. As a demonstration of this approach, [3H]BANA-CCK-8s was incubated with anti-CCK-antiserum and was subsequently passed over a streptavidin-agarose affinity matrix. After washing, the bound antibodies were eluted by photocleavage of the affinity ligand. Forty-six percent of the bound antibodies were recovered after 40 min of irradiation. The eluted antibodies showed essentially unchanged binding characteristics. The suitability of ANA-CCK-8s for the purification of anti-CCK antibodies was also demonstrated. The approach may prove to be generally useful in the isolation of labile proteins in an intact form.
Subject(s)
Biotin/metabolism , Chromatography, Affinity/methods , Photochemistry/methods , Amino Acid Sequence , Animals , Bacterial Proteins , Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Cholecystokinin/immunology , Cholecystokinin/metabolism , Ligands , Light , Molecular Sequence Data , Rabbits , Receptors, Cholecystokinin/metabolism , Sepharose , StreptavidinABSTRACT
A number of protected active peptide analogues of Cholecystokinin were prepared in solution by N-end stretching method with active esters. In the synthesis t-butoxycarbonyl (Boc-) and benzyloxycarbonyl (Z-) were applied as protecting groups. Six oligopeptides which contained protecting groups have not yet been reported in literature. Their structures were identified by amino acid analysis, opticity, IR and FAB-MS. The results of preliminary pharmacological tests show all the samples have biological activities in various degrees.
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Sincalide/chemical synthesis , Amino Acid Sequence , Cholecystokinin/chemistry , Molecular Sequence Data , Oligopeptides , Sincalide/chemistryABSTRACT
The synthesis of [Phe(p-CH2SO3Na)52, Nle32,53,56 Nal55]-CCK20-58, [Tyr52, Nle32,53,56, Nal55]-CCK-58 and of [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK-58 using the (9-fluorenylmethyloxy)-carbonyl (Fmoc) strategy on a 2,4-DMBHA resin is described. The crude peptide preparations were extremely complex when analyzed by RP-HPLC, capillary zone electrophoresis (CZE), and ion-exchange chromatography (IE-FPLC). We found that the most effective strategy for purification included cation-exchange chromatography followed by a RP-HPLC desalting step. The highly purified peptides (purity greater than 90%) were characterized by RP-HPLC, size exclusion HPLC (SEC), IE-FPLC, CZE, mass spectrometry, amino acid analysis, and Edman sequence analysis (for [Tyr52, Nle32,53,56, Nal55]-CCK-58). The results demonstrate the applicability of the 2,4-DMBHA resin for Fmoc solid-phase synthesis of long peptides amides (58 residues in length in this case) as well as the efficacy of an FPLC/RP-HPLC approach for the purification of very long, heterogeneous crude peptides, allowing a true assessment of the biological properties of these analogs to be carried out. [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK20-58 was less than 1% as potent as CCK-8 while [Tyr52, Nle32,53,56, Nal55]-CCK-58 and [Phe(p-CH2SO3Na)52, Nle32,53,56, Nal55]-CCK-58 were inactive at the doses tested (< 0.01%).
Subject(s)
Cholecystokinin/analogs & derivatives , Cholecystokinin/chemical synthesis , Amino Acid Sequence , Amylases/metabolism , Animals , Biological Assay , Cholecystokinin/isolation & purification , Cholecystokinin/pharmacology , Chromatography, High Pressure Liquid/methods , Chromatography, Liquid/methods , Humans , Indicators and Reagents , Molecular Sequence Data , Pancreas/drug effects , Pancreas/enzymology , RatsABSTRACT
New and existing methodologies were used to prepare a series of modified CCK analogs in which each amide bond was replaced by a trans-alkene unit. The data indicate that every amide linkage at C-terminal tetrapeptide (CCK-4) region is crucial for biological activity. While the amide bond beyond the Trp residue in the N-terminal direction can be replaced by a trans-alkene and still retain most of the binding potency and functional activity.
Subject(s)
Cholecystokinin/analogs & derivatives , Peptide Fragments/chemical synthesis , Amino Acid Sequence , Animals , Cerebral Cortex/metabolism , Cholecystokinin/chemical synthesis , Cholecystokinin/chemistry , Guinea Pigs , In Vitro Techniques , Magnetic Resonance Spectroscopy , Molecular Sequence Data , Molecular Structure , Pancreas/metabolism , Peptide Fragments/chemistry , Peptide Fragments/metabolism , Radioligand Assay , Structure-Activity RelationshipABSTRACT
Acidolytic deprotection and cleavage conditions for an acid-labile Tyr(SO3H)-containing peptide were systematically examined with respect to acid, temperature, and scavenger. The 90% aqueous trifluoroacetic acid (TFA)-based reagent systems (90% aqueous TFA/m-cresol and 90% aqueous TFA/m-cresol/2-methylindole) at 4 degrees C were found to minimize the deterioration of Tyr(SO3Na) in the peptide. The latter deprotection/cleavage system was applied to the 9-fluorenylmethyloxycarbonyl (Fmoc)-based solid-phase synthesis of cholecystokinin (CCK)-12 on an acid-labile PAL-linked support (PAL = peptide amide linker), with Fmoc-Tyr(SO3Na)-OH as a building block.