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1.
STAR Protoc ; 2(3): 100763, 2021 09 17.
Article in English | MEDLINE | ID: mdl-34467231

ABSTRACT

Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) techniques to examine changes in histone H2A ubiquitination patterns at various developmental stages. This protocol can be applied for both in vitro NPCs and NPCs directly isolated from mouse neocortices. For complete details on the use and execution of this protocol, please refer to (Tsuboi et al., 2018).


Subject(s)
Chromatin Immunoprecipitation/methods , Histones/metabolism , Neocortex/cytology , Neocortex/embryology , Neural Stem Cells/metabolism , Animals , Cell Culture Techniques/instrumentation , Cell Culture Techniques/methods , Cells, Cultured , Chromatin Immunoprecipitation/instrumentation , Female , Gene Expression Regulation, Developmental , Mice, Inbred C57BL , Mice, Inbred ICR , Neural Stem Cells/cytology , Pregnancy , Real-Time Polymerase Chain Reaction/methods , Ubiquitination
2.
Methods Mol Biol ; 1914: 145-167, 2019.
Article in English | MEDLINE | ID: mdl-30729464

ABSTRACT

Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.


Subject(s)
Bone and Bones/cytology , Chromatin Immunoprecipitation/methods , Electrophoretic Mobility Shift Assay/methods , Gene Expression Regulation , Transcription, Genetic , CRISPR-Cas Systems/genetics , Cell Culture Techniques/instrumentation , Cell Culture Techniques/methods , Cell Line, Tumor , Cell Separation/instrumentation , Cell Separation/methods , Chromatin Immunoprecipitation/instrumentation , Electrophoretic Mobility Shift Assay/instrumentation , Flow Cytometry/instrumentation , Flow Cytometry/methods , Gene Editing/instrumentation , Gene Editing/methods , Genes, Reporter/genetics , Humans , Promoter Regions, Genetic/genetics
3.
Anal Chem ; 90(12): 7666-7674, 2018 06 19.
Article in English | MEDLINE | ID: mdl-29842781

ABSTRACT

Genome-wide epigenetic changes, such as histone modifications, form a critical layer of gene regulations and have been implicated in a number of different disorders such as cancer and inflammation. Progress has been made to decrease the input required by gold-standard genome-wide profiling tools like chromatin immunoprecipitation followed by sequencing (i.e., ChIP-seq) to allow scarce primary tissues of a specific type from patients and lab animals to be tested. However, there has been practically no effort to rapidly increase the throughput of these low-input tools. In this report, we demonstrate LIFE-ChIP-seq (low-input fluidized-bed enabled chromatin immunoprecipitation followed by sequencing), an automated and high-throughput microfluidic platform capable of running multiple sets of ChIP assays on multiple histone marks in as little as 1 h with as few as 50 cells per assay. Our technology will enable testing of a large number of samples and replicates with low-abundance primary samples in the context of precision medicine.


Subject(s)
Automation , Chromatin Immunoprecipitation , Histones/chemistry , Histones/metabolism , Microfluidic Analytical Techniques , Cells, Cultured , Chromatin Immunoprecipitation/instrumentation , High-Throughput Screening Assays/instrumentation , Histones/genetics , Humans , Microfluidic Analytical Techniques/instrumentation
4.
Methods Mol Biol ; 1765: 43-56, 2018.
Article in English | MEDLINE | ID: mdl-29589300

ABSTRACT

Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.


Subject(s)
Chromatin Immunoprecipitation/methods , Colorectal Neoplasms/genetics , Integrin alpha1/genetics , Mutagenesis, Site-Directed/methods , Response Elements/genetics , Binding Sites , Carcinogenesis/genetics , Chromatin Immunoprecipitation/instrumentation , Computer Simulation , Gene Expression Regulation, Neoplastic , HEK293 Cells , Humans , Mutagenesis, Site-Directed/instrumentation , Proto-Oncogene Proteins c-myc/metabolism , Sequence Analysis, DNA/methods , Transcription Initiation Site
5.
J Vis Exp ; (124)2017 06 17.
Article in English | MEDLINE | ID: mdl-28654055

ABSTRACT

Signaling pathways regulate gene expression programs via the modulation of the chromatin structure at different levels, such as by post-translational modifications (PTMs) of histone tails, the exchange of canonical histones with histone variants, and nucleosome eviction. Such regulation requires the binding of signal-sensitive transcription factors (TFs) that recruit chromatin-modifying enzymes at regulatory elements defined as enhancers. Understanding how signaling cascades regulate enhancer activity requires a comprehensive analysis of the binding of TFs, chromatin modifying enzymes, and the occupancy of specific histone marks and histone variants. Chromatin immunoprecipitation (ChIP) assays utilize highly specific antibodies to immunoprecipitate specific protein/DNA complexes. The subsequent analysis of the purified DNA allows for the identification the region occupied by the protein recognized by the antibody. This work describes a protocol to efficiently perform ChIP of histone proteins in a mature mouse T-cell line. The presented protocol allows for the performance of ChIP assays in a reasonable timeframe and with high reproducibility.


Subject(s)
Chromatin Immunoprecipitation/methods , Chromatin/metabolism , Histones/metabolism , Regulatory Sequences, Nucleic Acid , T-Lymphocytes/metabolism , Animals , Cell Culture Techniques , Cell Line , Chromatin/genetics , Chromatin Immunoprecipitation/instrumentation , DNA/genetics , DNA/metabolism , DNA-Binding Proteins/genetics , DNA-Binding Proteins/metabolism , Histones/genetics , Humans , Mice , Nucleosomes/metabolism , Protein Binding , Protein Processing, Post-Translational , Reproducibility of Results , Transcription Factors/metabolism
6.
Lab Chip ; 17(3): 530-537, 2017 01 31.
Article in English | MEDLINE | ID: mdl-28092380

ABSTRACT

Genetic organization is governed by the interaction of DNA with histone proteins, and differential modifications of these proteins is a fundamental mechanism of gene regulation. Histone modifications are primarily studied through chromatin immunoprecipitation (ChIP) assays, however conventional ChIP procedures are time consuming, laborious and require a large number of cells. Here we report for the first time the development of ChIP in droplets based on a microfluidic platform combining nanoliter droplets, magnetic beads (MB) and magnetic tweezers (MT). The droplet approach enabled compartmentalization and improved mixing, while reducing the consumption of samples and reagents in an integrated workflow. Anti-histone antibodies grafted to MB were used as a solid support to capture and transfer the target chromatin from droplets to droplets in order to perform chromatin immunoprecipitation, washing, elution and purification of DNA. We designed a new ChIP protocol to investigate four different types of modified histones with known roles in gene activation or repression. We evaluated the performances of this new ChIP in droplet assay in comparison with conventional methods. The proposed technology dramatically reduces analytical time from a few days to 7 hours, simplifies the ChIP protocol and decreases the number of cells required by 100 fold while maintaining a high degree of sensitivity and specificity. Therefore this droplet-based ChIP assay represents a new, highly advantageous and convenient approach to epigenetic analyses.


Subject(s)
Chromatin Immunoprecipitation/instrumentation , Chromatin Immunoprecipitation/methods , Chromatin/isolation & purification , Microfluidic Analytical Techniques/instrumentation , Cell Line, Tumor , Chromatin/chemistry , Equipment Design , Histones/analysis , Histones/chemistry , Histones/genetics , Histones/isolation & purification , Humans , Sensitivity and Specificity
7.
Nat Methods ; 12(10): 963-965, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26280331

ABSTRACT

Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is widely used to map histone marks and transcription factor binding throughout the genome. Here we present ChIPmentation, a method that combines chromatin immunoprecipitation with sequencing library preparation by Tn5 transposase ('tagmentation'). ChIPmentation introduces sequencing-compatible adaptors in a single-step reaction directly on bead-bound chromatin, which reduces time, cost and input requirements, thus providing a convenient and broadly useful alternative to existing ChIP-seq protocols.


Subject(s)
Chromatin Immunoprecipitation/methods , Histones/metabolism , Transcription Factors/metabolism , Chromatin Immunoprecipitation/economics , Chromatin Immunoprecipitation/instrumentation , Genome, Human , Humans , K562 Cells , Transcription Factors/analysis
8.
Nat Methods ; 12(10): 959-62, 2015 Oct.
Article in English | MEDLINE | ID: mdl-26214128

ABSTRACT

The sensitivity of chromatin immunoprecipitation (ChIP) assays poses a major obstacle for epigenomic studies of low-abundance cells. Here we present a microfluidics-based ChIP-seq protocol using as few as 100 cells via drastically improved collection of high-quality ChIP-enriched DNA. Using this technology, we uncovered many new enhancers and super enhancers in hematopoietic stem and progenitor cells from mouse fetal liver, suggesting that enhancer activity is highly dynamic during early hematopoiesis.


Subject(s)
Chromatin Immunoprecipitation/instrumentation , Chromatin Immunoprecipitation/methods , Epigenomics/instrumentation , Lab-On-A-Chip Devices , Acetylation , Animals , Enhancer Elements, Genetic , Epigenomics/methods , Equipment Design , Female , Hematopoiesis , Hematopoietic Stem Cells/physiology , Histones/metabolism , Humans , Liver/embryology , Liver/physiology , Male , Manufacturing Industry/methods , Mice, Inbred C57BL
9.
Genes Cells ; 19(12): 919-26, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25324176

ABSTRACT

Raw data from ChIP-seq (chromatin immunoprecipitation combined with massively parallel DNA sequencing) experiments are deposited in public databases as SRAs (Sequence Read Archives) that are publically available to all researchers. However, to graphically visualize ChIP-seq data of interest, the corresponding SRAs must be downloaded and converted into BigWig format, a process that involves complicated command-line processing. This task requires users to possess skill with script languages and sequence data processing, a requirement that prevents a wide range of biologists from exploiting SRAs. To address these challenges, we developed SraTailor, a GUI (Graphical User Interface) software package that automatically converts an SRA into a BigWig-formatted file. Simplicity of use is one of the most notable features of SraTailor: entering an accession number of an SRA and clicking the mouse are the only steps required to obtain BigWig-formatted files and to graphically visualize the extents of reads at given loci. SraTailor is also able to make peak calls, generate files of other formats, process users' own data, and accept various command-line-like options. Therefore, this software makes ChIP-seq data fully exploitable by a wide range of biologists. SraTailor is freely available at http://www.devbio.med.kyushu-u.ac.jp/sra_tailor/, and runs on both Mac and Windows machines.


Subject(s)
Chromatin Immunoprecipitation/instrumentation , Electronic Data Processing , Software , User-Computer Interface , Sequence Analysis, DNA
10.
Methods Mol Biol ; 1204: 25-34, 2014.
Article in English | MEDLINE | ID: mdl-25182758

ABSTRACT

Better defining the dynamics of biomolecular interactions is an important step in understanding molecular biology and cellular processes. DNA-protein interactions, and specifically hormone-triggered DNA-nuclear receptor interactions, are key events which are still poorly understood. To date, the most commonly used approach in studying chromatin interactions is the immunoprecipitation of chemically cross-linked chromatin (ChIP) coupled with single gene or global genomic analyses. Currently, establishing a stable interplay between nucleic acids and proteins (DNA-protein cross-link) is mainly obtained through conventional, diffusion-triggered, chemical methods using formaldehyde. Here we describe an alternative method, called Laser-ChIP (LChIP), for the specific analysis of interactions between chromatin and nuclear receptors driven by a UV laser energy source. Photo-induced cross-linking in LChIP is achieved very rapidly, allowing the study of transient interactions, depending on laser source parameters.


Subject(s)
Chromatin Immunoprecipitation/instrumentation , Chromatin/metabolism , Lasers , Receptors, Cytoplasmic and Nuclear/metabolism , Animals , Cell Cycle , Cell Death , Chromatin Immunoprecipitation/methods , DNA/metabolism , Equipment Design , Humans
11.
Genome Biol ; 14(11): R124, 2013 Nov 07.
Article in English | MEDLINE | ID: mdl-24200198

ABSTRACT

ChIP-seq is an established manually-performed method for identifying DNA-protein interactions genome-wide. Here, we describe a protocol for automated high-throughput (AHT) ChIP-seq. To demonstrate the quality of data obtained using AHT-ChIP-seq, we applied it to five proteins in mouse livers using a single 96-well plate, demonstrating an extremely high degree of qualitative and quantitative reproducibility among biological and technical replicates. We estimated the optimum and minimum recommended cell numbers required to perform AHT-ChIP-seq by running an additional plate using HepG2 and MCF7 cells. With this protocol, commercially available robotics can perform four hundred experiments in five days.


Subject(s)
Chromatin Immunoprecipitation/methods , High-Throughput Nucleotide Sequencing/methods , Robotics/methods , Animals , Chromatin Immunoprecipitation/instrumentation , Hep G2 Cells , High-Throughput Nucleotide Sequencing/instrumentation , Humans , Liver/metabolism , MCF-7 Cells , Male , Mice , Mice, Inbred C57BL , Reproducibility of Results , Robotics/instrumentation , Sequence Analysis, DNA
12.
Methods Mol Biol ; 1067: 225-33, 2013.
Article in English | MEDLINE | ID: mdl-23975795

ABSTRACT

The recent revolution of genomics techniques has allowed the detection of various sequence features and biological variations on whole-genome scale. However, these high-resolution data present significant challenges for experimental biologists to understand and analyze. The conventional way is to use genome browsers to locate and visualize regions of interest. But it lacks user-friendly data mining functionality. Here we present a protocol that allows rapid annotation of genomic coordinate data by using TileMapper. Interesting biological annotations from large-scale genomic data, such as transcriptome analysis, chromatin immunoprecipitation on chip, or methyl-DNA immunoprecipitation (MeDIP) studies generated from the tiling microarrays and other platforms, could be analyzed without requiring computational skills. The outputs are saved in tabulated format, which permit flexible and simple processing in spreadsheet software, or to be exported to other pipelines for subsequent analysis.


Subject(s)
Chromatin Immunoprecipitation/methods , Chromosome Mapping/methods , Genomics/methods , Oligonucleotide Array Sequence Analysis/methods , Animals , Chromatin Immunoprecipitation/instrumentation , Chromosome Mapping/instrumentation , Databases, Genetic , Genome , Genomics/instrumentation , Humans , Oligonucleotide Array Sequence Analysis/instrumentation , Software
13.
J Vis Exp ; (74)2013 Apr 19.
Article in English | MEDLINE | ID: mdl-23629434

ABSTRACT

ChIP-sequencing (ChIP-seq) methods directly offer whole-genome coverage, where combining chromatin immunoprecipitation (ChIP) and massively parallel sequencing can be utilized to identify the repertoire of mammalian DNA sequences bound by transcription factors in vivo. "Next-generation" genome sequencing technologies provide 1-2 orders of magnitude increase in the amount of sequence that can be cost-effectively generated over older technologies thus allowing for ChIP-seq methods to directly provide whole-genome coverage for effective profiling of mammalian protein-DNA interactions. For successful ChIP-seq approaches, one must generate high quality ChIP DNA template to obtain the best sequencing outcomes. The description is based around experience with the protein product of the gene most strongly implicated in the pathogenesis of type 2 diabetes, namely the transcription factor transcription factor 7-like 2 (TCF7L2). This factor has also been implicated in various cancers. Outlined is how to generate high quality ChIP DNA template derived from the colorectal carcinoma cell line, HCT116, in order to build a high-resolution map through sequencing to determine the genes bound by TCF7L2, giving further insight in to its key role in the pathogenesis of complex traits.


Subject(s)
Chromatin Immunoprecipitation/methods , Colorectal Neoplasms/chemistry , DNA, Neoplasm/isolation & purification , High-Throughput Nucleotide Sequencing/methods , Chromatin Immunoprecipitation/instrumentation , Colorectal Neoplasms/genetics , DNA, Neoplasm/genetics , DNA, Neoplasm/metabolism , HCT116 Cells , High-Throughput Nucleotide Sequencing/instrumentation , Humans , Transcription Factor 7-Like 2 Protein/chemistry , Transcription Factor 7-Like 2 Protein/genetics , Transcription Factor 7-Like 2 Protein/metabolism
14.
BMC Mol Biol ; 12: 49, 2011 Nov 18.
Article in English | MEDLINE | ID: mdl-22098709

ABSTRACT

BACKGROUND: The processes that compose expression of a given gene are far more complex than previously thought presenting unprecedented conceptual and mechanistic challenges that require development of new tools. Chromatin structure, which is regulated by DNA methylation and histone modification, is at the center of gene regulation. Immunoprecipitations of chromatin (ChIP) and methylated DNA (MeDIP) represent a major achievement in this area that allow researchers to probe chromatin modifications as well as specific protein-DNA interactions in vivo and to estimate the density of proteins at specific sites genome-wide. Although a critical component of chromatin structure, DNA methylation has often been studied independently of other chromatin events and transcription. RESULTS: To allow simultaneous measurements of DNA methylation with other genomic processes, we developed and validated a simple and easy-to-use high throughput microplate-based platform for analysis of DNA methylation. Compared to the traditional beads-based MeDIP the microplate MeDIP was more sensitive and had lower non-specific binding. We integrated the MeDIP method with a microplate ChIP assay which allows measurements of both DNA methylation and histone marks at the same time, Matrix ChIP-MeDIP platform. We illustrated several applications of this platform to relate DNA methylation, with chromatin and transcription events at selected genes in cultured cells, human cancer and in a model of diabetic kidney disease. CONCLUSION: The high throughput capacity of Matrix ChIP-MeDIP to profile tens and potentially hundreds of different genomic events at the same time as DNA methylation represents a powerful platform to explore complex genomic mechanism at selected genes in cultured cells and in whole tissues. In this regard, Matrix ChIP-MeDIP should be useful to complement genome-wide studies where the rich chromatin and transcription database resources provide fruitful foundation to pursue mechanistic, functional and diagnostic information at genes of interest in health and disease.


Subject(s)
Chromatin Immunoprecipitation/methods , Proteins/metabolism , Animals , Cell Line , Chromatin/genetics , Chromatin/metabolism , Chromatin Immunoprecipitation/instrumentation , DNA/genetics , DNA/metabolism , DNA Methylation , High-Throughput Screening Assays/methods , Humans , Male , Mice , Mice, Inbred C57BL , Proteins/genetics
15.
Methods Enzymol ; 497: 51-73, 2011.
Article in English | MEDLINE | ID: mdl-21601082

ABSTRACT

Chromatin immunoprecipitation coupled with ultra-high-throug put parallel DNA sequencing (ChIP-seq) is an effective technology for the investigation of genome-wide protein-DNA interactions. Examples of applications include the studies of RNA polymerases transcription, transcriptional regulation, and histone modifications. The technology provides accurate and high-resolution mapping of the protein-DNA binding loci that are important in the understanding of many processes in development and diseases. Since the introduction of ChIP-seq experiments in 2007, many statistical and computational methods have been developed to support the analysis of the massive datasets from these experiments. However, because of the complex, multistaged analysis workflow, it is still difficult for an experimental investigator to conduct the analysis of his or her own ChIP-seq data. In this chapter, we review the basic design of ChIP-seq experiments and provide an in-depth tutorial on how to prepare, to preprocess, and to analyze ChIP-seq datasets. The tutorial is based on a revised version of our software package CisGenome, which was designed to encompass most standard tasks in ChIP-seq data analysis. Relevant statistical and computational issues will be highlighted, discussed, and illustrated by means of real data examples.


Subject(s)
Chromatin Immunoprecipitation/methods , Sequence Analysis, DNA/methods , Base Sequence , Chromatin Immunoprecipitation/instrumentation , Computational Biology/instrumentation , Computational Biology/methods , Databases, Nucleic Acid , Genome , Humans , Sequence Analysis, DNA/instrumentation , Software
16.
Methods Mol Biol ; 712: 45-58, 2011.
Article in English | MEDLINE | ID: mdl-21359799

ABSTRACT

The completion of the alfalfa, Arabidopsis, papaya, poplar, and rice genome sequences along with ongoing sequencing projects of various crop species, offers an excellent opportunity to study gene expression at the whole genome level and to unravel the complexity of gene networks underlying the reprogramming of plant defense toward pathogen challenge. Gene expression in eukaryotic cells is mainly controlled by regulatory elements that recruit transcription factors (TFs) to modulate transcriptional outputs. Therefore, methods allowing the identification of all cognate TF binding sites (TFBS) within the regulatory regions of target genes on a genome-wide basis are the next obvious step to elucidate the plant defense transcriptome. Chromatin immunoprecipitation (ChIP) is one such powerful technique for analyzing functional cis-regulatory DNA elements. The ChIP assay allows the identification of specific regulatory DNA regions associated with trans-acting regulatory factors in vivo. ChIP assays can provide spatial and temporal snapshots of the regulatory components involved in reprogramming host gene expression upon pathogen ingress. Moreover, the use of ChIP-enriched DNA for hybridization to tiling microarrays (ChIP-chip) or for direct sequencing (ChIP-Seq) by means of massively parallel sequencing technology has expanded this methodology to address global changes in gene expression.


Subject(s)
Chromatin Immunoprecipitation/methods , Plant Immunity , Plant Proteins/isolation & purification , Transcription Factors/isolation & purification , Animals , Arabidopsis/anatomy & histology , Arabidopsis/genetics , Arabidopsis/immunology , Arabidopsis/microbiology , Chromatin Immunoprecipitation/instrumentation , DNA, Plant/analysis , Immunomagnetic Separation , Plant Leaves/immunology , Plant Leaves/microbiology , Plant Proteins/genetics , Plant Proteins/immunology , Transcription Factors/immunology
17.
Respirology ; 16(2): 210-22, 2011 Feb.
Article in English | MEDLINE | ID: mdl-21077988

ABSTRACT

Next generation sequencing (NGS) has pushed back the limitations of prior sequencing technologies to advance genomic knowledge infinitely by allowing cost-effective, rapid sequencing to become a reality. Genome-wide transcriptional profiling can be achieved using NGS with either Tag-Seq, in which short tags of cDNA represent a gene, or RNA-Seq, in which the entire transcriptome is sequenced. Furthermore, the level and diversity of miRNA within different tissues or cell types can be monitored by specifically sequencing small RNA. The biological mechanisms underlying differential gene regulation can also be explored by coupling chromatin immunoprecipitation with NGS (ChIP-Seq). Using this methodology genome-wide binding sites for transcription factors, RNAP II, epigenetic modifiers and the distribution of modified histones can be assessed. The superior, high-resolution data generated by adopting this sequencing technology allows researchers to distinguish the precise genomic location bound by a protein and correlate this with observed gene expression patterns. Additional methods have also been established to examine other factors influencing gene regulation such as DNA methylation or chromatin conformation on a genome-wide scale. Within any research setting, these techniques can provide relevant data and answer numerous questions about gene expression and regulation. The advances made by pairing NGS with strategic experimental protocols will continue to impact the research community.


Subject(s)
Gene Expression Profiling/methods , Gene Expression Regulation , Sequence Analysis/methods , Base Sequence , Chromatin/genetics , Chromatin Immunoprecipitation/instrumentation , Chromatin Immunoprecipitation/methods , DNA Methylation/genetics , Gene Expression Profiling/instrumentation , Histones/chemistry , Histones/genetics , Humans , RNA Polymerase II/genetics , RNA, Small Interfering/genetics , Sequence Analysis/instrumentation , Transcription Factors/genetics
18.
Methods Mol Biol ; 582: 103-18, 2009.
Article in English | MEDLINE | ID: mdl-19763945

ABSTRACT

Here we describe an adapted ChIP-on-chip protocol for the analysis of DNA topoisomerase chromosomal binding in Saccharomyces cerevisiae cells. The ChIP-on-chip technique is based on the immunoprecipitation of crosslinked chromatin (ChIP, chromatin immunoprecipitation), followed by DNA amplification and hybridization to high-density oligonucleotide arrays (Chip). Comparison of the signal intensities of immunoprecipitated and control fractions provides a measurement of the protein-DNA association along entire genomes. ChIP-on-chip analysis of DNA topoisomerase binding to chromosomal DNA opens a window to the understanding of the in vivo contribution of these enzymes to the different DNA transactions taking place concomitantly within the context of the highly organized eukaryotic genome. Chromosomal binding profiles obtained from synchronized cells allow scoring the temporal and spatial restriction of these enzymes at different cell cycle stages. By using this approach, novel aspects of DNA topoisomerase function in chromosome metabolism might be unmasked.


Subject(s)
Chromatin Immunoprecipitation , DNA Topoisomerases/metabolism , Oligonucleotide Array Sequence Analysis , Amino Acid Sequence , Animals , Chromatin Immunoprecipitation/instrumentation , Chromatin Immunoprecipitation/methods , Chromosomes, Fungal/genetics , Chromosomes, Fungal/metabolism , DNA/chemistry , DNA/genetics , DNA/metabolism , Models, Genetic , Nucleic Acid Conformation , Oligonucleotide Array Sequence Analysis/instrumentation , Oligonucleotide Array Sequence Analysis/methods , Protein Binding , Saccharomyces cerevisiae/enzymology , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
19.
Methods Mol Biol ; 582: 119-30, 2009.
Article in English | MEDLINE | ID: mdl-19763946

ABSTRACT

In vertebrate cells, DNA topoisomerase II (Topo II), named Top2 in yeast, localizes along chromosome axes early in mitosis and concentrates within centromeric chromatin during metaphase. The factors controlling these changes in enzyme distribution are largely unknown. Insight into Topo II dynamics could potentially be derived through genetic approaches in yeast. In practice, however, the small size and limited compaction of yeast chromosomes has precluded a detailed analysis of Top2 localization along mitotic chromosomes. As an alternative approach, we describe a method for examining Top2 distribution using chromatin immunoprecipitation (ChIP). By adding a detergent solubilization step, this method allows efficient recovery of DNA sequences associated with Top2 in the insoluble chromosome scaffold fraction.


Subject(s)
Chromatin Immunoprecipitation/methods , Chromosomes, Fungal/metabolism , DNA Topoisomerases, Type II/metabolism , Chromatin/genetics , Chromatin/metabolism , Chromatin Immunoprecipitation/instrumentation , Chromosomes, Fungal/genetics , DNA Topoisomerases, Type II/genetics , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism
20.
Methods Mol Biol ; 567: 27-43, 2009.
Article in English | MEDLINE | ID: mdl-19588083

ABSTRACT

We present here the very robust characterization and quality control (QC) process that we have established for our polyclonal antibodies, which are mainly directed against targets relevant to the epigenetics field such as modified histones, modifying enzymes, and chromatin-interacting proteins. The final purpose of the characterization and QC is to label antibodies as chromatin immunoprecipitation (ChIP) grade. Indeed, the ChIP method is extensively used in epigenetics to study gene regulation and relies on the use of antibodies to select the protein of interest and then precipitate and identify the DNA associated to it. We have optimized in-house all protocols and reagents needed from the first to the last step of antibody characterization. First, following immunizations, the rabbit crude serum is tested for immune response. Whether or not the antibody is specific is determined in further characterizations. Then, only specific antibodies are tested in ChIP using an optimized method which is ideal for antibody screening. Once QC is established for one antibody, it is used to similarly characterize each antibody batch in order to supply researchers in a reproducible manner with validated antibodies. All in all, this demonstrates that we develop epigenetics research tools based on everyday's researcher's needs by providing batch-specific fully characterized ChIP-grade antibodies.


Subject(s)
Antibodies/pharmacology , Chromatin Immunoprecipitation/methods , Chromatin Immunoprecipitation/standards , Animals , Blotting, Western/methods , Chromatin Immunoprecipitation/instrumentation , Enzyme-Linked Immunosorbent Assay/methods , Humans , Quality Control , Rabbits
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