ABSTRACT
Inflammatory cardiomyopathy, characterized by inflammatory cell infiltration into the myocardium and a high risk of deteriorating cardiac function, has a heterogeneous aetiology. Inflammatory cardiomyopathy is predominantly mediated by viral infection, but can also be induced by bacterial, protozoal or fungal infections as well as a wide variety of toxic substances and drugs and systemic immune-mediated diseases. Despite extensive research, inflammatory cardiomyopathy complicated by left ventricular dysfunction, heart failure or arrhythmia is associated with a poor prognosis. At present, the reason why some patients recover without residual myocardial injury whereas others develop dilated cardiomyopathy is unclear. The relative roles of the pathogen, host genomics and environmental factors in disease progression and healing are still under discussion, including which viruses are active inducers and which are only bystanders. As a consequence, treatment strategies are not well established. In this Review, we summarize and evaluate the available evidence on the pathogenesis, diagnosis and treatment of myocarditis and inflammatory cardiomyopathy, with a special focus on virus-induced and virus-associated myocarditis. Furthermore, we identify knowledge gaps, appraise the available experimental models and propose future directions for the field. The current knowledge and open questions regarding the cardiovascular effects associated with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are also discussed. This Review is the result of scientific cooperation of members of the Heart Failure Association of the ESC, the Heart Failure Society of America and the Japanese Heart Failure Society.
Subject(s)
Cardiomyopathies/physiopathology , Inflammation/physiopathology , Myocarditis/physiopathology , Virus Diseases/physiopathology , Animals , Antiviral Agents/therapeutic use , Autoimmunity/immunology , Biopsy , COVID-19/physiopathology , COVID-19/therapy , Cardiomyopathies/diagnosis , Cardiomyopathies/immunology , Cardiomyopathies/therapy , Cardiomyopathy, Dilated , Coronavirus Infections/immunology , Coronavirus Infections/physiopathology , Coronavirus Infections/therapy , Coxsackievirus Infections/immunology , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/therapy , Cytomegalovirus Infections/immunology , Cytomegalovirus Infections/physiopathology , Cytomegalovirus Infections/therapy , Disease Models, Animal , Echovirus Infections/immunology , Echovirus Infections/physiopathology , Echovirus Infections/therapy , Epstein-Barr Virus Infections/immunology , Epstein-Barr Virus Infections/physiopathology , Epstein-Barr Virus Infections/therapy , Erythema Infectiosum/immunology , Erythema Infectiosum/physiopathology , Erythema Infectiosum/therapy , HIV Infections/physiopathology , Hepatitis C/immunology , Hepatitis C/physiopathology , Hepatitis C/therapy , Humans , Immunoglobulins, Intravenous/therapeutic use , Immunologic Factors/therapeutic use , Inflammation/diagnosis , Inflammation/immunology , Inflammation/therapy , Influenza, Human/immunology , Influenza, Human/physiopathology , Influenza, Human/therapy , Leukocytes/immunology , Myocarditis/diagnosis , Myocarditis/immunology , Myocarditis/therapy , Myocardium/pathology , Prognosis , Roseolovirus Infections/immunology , Roseolovirus Infections/physiopathologyABSTRACT
BACKGROUND: Coxsackie-B-viruses (CVB) are frequent causes of acute myocarditis and dilated cardiomyopathy, but an effective antiviral therapy is still not available. Previously, we and others have demonstrated that treatment with an engineered sCAR-Fc (soluble coxsackievirus-adenovirus receptor fused to the carboxyl-terminus of human IgG) efficiently neutralizes CVB3 and inhibits the development of cardiac dysfunction in mice with acute CVB3-induced myocarditis. In this study, we analyzed the potential of sCAR-Fc for treatment of chronic CVB3-induced myocarditis in an outbred NMRI mouse model. METHODS: NMRI mice were infected with the CVB3 strain 31-1-93 and treated with a sCAR-Fc expressing adeno-associated virus 9 vector 1, 3, and 7 days after CVB3 infection. Chronic myocarditis was analyzed on day 28 after infection. RESULTS: Initial investigations showed that NMRI mice develop pronounced chronic myocarditis between day 18 and day 28 after infection with the CVB3 strain 31-1-93. Chronic cardiac infection was characterized by inflammation and fibrosis as well as persistence of viral genomes in the heart tissue and by cardiac dysfunction. Treatment of NMRI mice resulted in a distinct reduction of cardiac inflammation and fibrosis and almost complete elimination of virus RNA from the heart by day 28 after infection. Moreover, hemodynamic measurement revealed improved cardiac contractility and diastolic relaxation in treated mice compared with mice treated with a control vector (mean±SD; maximal pressure, 81.9±9.2 versus 69.4±8.6 mm Hg, P=0.02; left ventricular ejection fraction, 68.9±8.5 versus 54.2±11.5%, P=0.02; dP/dtmax, 7275.2±1674 versus 4432.6±1107 mm Hg/s, P=0.004; dP/dtmin, -4046.9±776 versus -3146.3±642 mm Hg/s, P=0.046). The therapeutic potential of sCAR-Fc is limited, however, since postponed start of sCAR-Fc treatment either 3 or 7 days after infection could not attenuate myocardial injury. CONCLUSIONS: Early therapeutic employment of sCAR-Fc, initiated at the beginning of the primary viremia, inhibits the development of chronic CVB3-induced myocarditis and improves the cardiac function to a level equivalent to that of uninfected animals.
Subject(s)
Antiviral Agents/administration & dosage , Cardiomyopathies/drug therapy , Coxsackievirus Infections/drug therapy , Enterovirus B, Human/drug effects , Immunoconjugates/administration & dosage , Immunoglobulin G/administration & dosage , Myocarditis/drug therapy , Receptors, Virus/administration & dosage , Animals , Cardiomyopathies/pathology , Cardiomyopathies/physiopathology , Cardiomyopathies/virology , Chronic Disease , Coxsackievirus Infections/pathology , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/virology , Disease Models, Animal , Enterovirus B, Human/pathogenicity , Fibrosis , Male , Mice , Myocarditis/pathology , Myocarditis/virology , Myocardium/pathology , Recombinant Fusion Proteins/adverse effects , Ventricular Function, Left , Viral LoadABSTRACT
Rituximab and other B cell depleting agents are increasingly used for haematological, immunological and neurological diseases. In a small minority, immunosuppression leads to increased virulence of normally mild infections. Brainstem encephalitis has been described occurring after infection from enteroviruses, more commonly in the paediatric population, but also in immunosuppressed adults. In this paper, we describe an enteroviral brainstem encephalitis in a rituximab-immunosuppressed patient. The enterovirus identified was Coxsackie A16, which has never yet been reported to cause brainstem encephalitis in an adult.
Subject(s)
Cerebrospinal Fluid/virology , Coxsackievirus Infections , Encephalitis , Enterovirus/isolation & purification , Immunoglobulins, Intravenous/administration & dosage , Rituximab , Adult , Antineoplastic Agents, Immunological/administration & dosage , Antineoplastic Agents, Immunological/adverse effects , Antineoplastic Agents, Immunological/immunology , Antineoplastic Combined Chemotherapy Protocols/administration & dosage , Brain Stem/diagnostic imaging , Brain Stem/virology , Coxsackievirus Infections/diagnosis , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/therapy , Cyclophosphamide/administration & dosage , Doxorubicin/administration & dosage , Encephalitis/diagnosis , Encephalitis/physiopathology , Encephalitis/therapy , Encephalitis/virology , Humans , Immunocompromised Host , Immunologic Factors/administration & dosage , Lymphoma, Follicular/drug therapy , Magnetic Resonance Imaging/methods , Male , Prednisone/administration & dosage , Rituximab/administration & dosage , Rituximab/adverse effects , Rituximab/immunology , Treatment Outcome , Vincristine/administration & dosageABSTRACT
Coxsackievirus-B4 (CV-B4) E2 can persist in the pancreatic ductal-like cells (Panc-1 cell line), which results in an impaired differentiation of these cells into islet-like cell aggregates (ICA). In this study, primary pancreatic ductal cells obtained as a by-product of islet isolation from the pancreas of seven brain-dead adults were inoculated with CV-B4 E2, followed-up for 29 days, and the impact was investigated. Viral titers in culture supernatants were analyzed throughout the culture. Intracellular viral RNA was detected by RT-PCR. Levels of ductal cell marker CK19 mRNA and of insulin mRNA were evaluated by qRT-PCR. The concentration of c-peptide in supernatants was determined by ELISA. Ductal cells exposed to trypsin and serum-free medium formed ICA and resulted in an increased insulin secretion. Ductal cells from five brain-dead donors were severely damaged by CV-B4 E2, whereas the virus persisted in cultures of cells obtained from the other two. The ICAs whose formation was induced on day 14 post-inoculation were scarce and appeared tiny in infected cultures. Also, insulin mRNA expression and c-peptide levels were strongly reduced compared to the controls. In conclusion, CV-B4 E2 lysed human primary pancreatic ductal cells or persisted in these cells, which resulted in the impairment of differentiation into insulin-producing cells.
Subject(s)
Coxsackievirus Infections/virology , Enterovirus B, Human/physiology , Insulin-Secreting Cells/metabolism , Insulin/metabolism , Pancreatic Ducts/virology , Cell Differentiation , Cells, Cultured , Coxsackievirus Infections/metabolism , Coxsackievirus Infections/physiopathology , Enterovirus B, Human/genetics , Epithelial Cells , Humans , Insulin-Secreting Cells/cytologyABSTRACT
Apoptosis plays a crucial role in regulating cardiomyopathy and injuries of coxsackievirus B3 (CVB3)-induced viral myocarditis (VM). It has been reported that Astragaloside IV (AST-IV) from Astragalus membranaceus could inhibit apoptosis under a variety of pathological conditions in vivo or in vitro. However, the functional roles of AST-IV in CVB3-induced VM still remain unknown. Here, we found that AST-IV significantly enhanced survival for CVB3-induced mice. AST-IV protected the mice against CVB3-induced virus myocarditis characterized by the increased body weight, decreased serum level of creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH), supressed expression of Ifn-γ, Il-6 in heart, enhanced systolic and diastolic function of left ventricle. At the pathological level, AST-IV ameliorated the mice against CVB3-induced myocardial damage and myocardial fibrosis. In vitro, the results from flow cytometry showed that AST-IV significantly suppressed CVB3-induced cardiomyocytes apoptosis, which also were verified in vivo. Moreover, an increased expression of pro-apoptotic genes including FAS, FASL, cleaved caspase-8 and cleaved caspase-3 was found in CVB3-induced cardiomyocytes, while those was inhibited in cardiomyocytes treated with AST-IV. Taken together, the data suggest that AST-IV protected against CVB3-induced myocardial damage and fibrosis, which may partly attribute to supress activation of FAS/FASL signaling pathway.
Subject(s)
Apoptosis/drug effects , Coxsackievirus Infections/physiopathology , Myocarditis/physiopathology , Myocytes, Cardiac/drug effects , Protective Agents/pharmacology , Saponins/pharmacology , Triterpenes/pharmacology , Animals , Apoptosis/physiology , Astragalus propinquus/chemistry , Disease Models, Animal , Enterovirus B, Human/physiology , Male , Mice , Mice, Inbred C57BL , Myocytes, Cardiac/physiology , Random AllocationABSTRACT
INTRODUCTION: Coxsackievirus B3 (CVB3) is known to induce acute and chronic myocarditis. Most infections are clinically unapparent but some patients suffer from ventricular arrhythmias (VA) and sudden cardiac death (SCD). Studies showed that acute CVB3 infection may cause impaired function of cardiac ion channels, creating a proarrhythmic substrate. However, it is unknown whether low level CVB3+ expression in myocytes may cause altered cardiac electrophysiology leading to VA. METHODS: Cellular electrophysiology was used to analyze cellular action potentials (APs) and occurrence of afterdepolarizations from isolated cardiomyocytes of wildtype (WT) and transgenic CVB3ΔVP0 (CVB3+) mice. Further, we studied surface ECGs, monophasic APs, ventricular effective refractory period (VERP) and inducibility of VAs in Langendorff-perfused whole hearts. All used cardiomyocytes and whole hearts originated from male mice. RESULTS: Cellular action potential duration (APD) in WT and CVB3+ myocytes was unchanged. No difference in mean occurrence or amplitude of afterdepolarizations in WT and CVB3+ myocytes was found. Interestingly, resting membrane potential in CVB3+ myocytes was significantly hyperpolarized (WT: -90.0±2.2 mV, n = 7; CVB3+: -114.1±3.0 mV, n = 14; p<0.005). Consistently, in Langendorff-perfused hearts, APDs were also not different between WT and CVB3+ whole hearts. Within both groups, we found a heart rate dependent shortening of ADP90 with increasing heart rate in Langendorff-perfused hearts. VERP was significantly prolonged in CVB3+ hearts compared to WT (WT: 36.0±2.7 ms, n = 5; CVB3+: 47.0±2.0 ms, n = 7; p = 0.018). Resting heart rate (HR) in Langendorff-perfused hearts was not significantly different between both genotypes. Electrical pacing protocols induced no VA in WT and CVB3+ hearts. CONCLUSION: In CVB3+ mice, prolonged ventricular refractoriness and hyperpolarized resting membrane potentials in presence of unchanged APD were observed, suggesting that low level CVB3 expression does not promote VA by altered cardiac electrophysiology in this type of chronic myocarditis. These findings may suggest that other mechanisms such as chronic myocardial inflammation or fibrosis may account for arrhythmias observed in patients with chronic enteroviral myocarditis.
Subject(s)
Coxsackievirus Infections/physiopathology , Myocarditis/physiopathology , Myocarditis/virology , Myocytes, Cardiac/physiology , Myocytes, Cardiac/virology , Animals , Arrhythmias, Cardiac/physiopathology , Arrhythmias, Cardiac/virology , Cells, Cultured , Chronic Disease , Disease Models, Animal , Electrophysiological Phenomena , Heart Rate/physiology , Male , Membrane Potentials , Mice, Inbred C57BL , Mice, Transgenic , Patch-Clamp Techniques , Tissue Culture Techniques , Viral Proteins/genetics , Viral Proteins/metabolismABSTRACT
In previous studies it was shown that inoculation of Swiss albino mice with CV-B4 E2 resulted in the production of serum IgG capable of enhancing the CV-B4 E2 infection of murine spleen cells cultures. To investigate whether such an enhancing activity of serum can play a role in vivo, we decided to study the CV-B4 E2 infection in mice exposed to successive inoculations of virus. In Swiss albino mice infected with CV-B4 E2 at the age of 21 days, anti-CV-B4 E2 neutralizing and enhancing activities of their serum peaked after 55 d. In contrast, mice inoculated at the age of 55 d expressed much lower activities. Despite the neutralizing activity of serum, CV-B4 E2 inoculated a second time to 55 day-old animals spread into the host. At the age of 72 and 89 d the levels of viral RNA and infectious particles were higher in organs of animals exposed to 2 successive infections compared with animals infected once at the age of 21 d or 55 d. In animals with 2 successive inoculations of CV-B4 E2 there was a relationship between the anti-CV-B4 E2 enhancing activity of serum and the level of viral RNA in organs and an enhancement of pathology was observed as displayed by histological analysis of pancreas and hyperglycaemia. Altogether our data strongly suggest that an anti-CV-B4 E2 enhancing activity in the host can play a role in the outcome of a secondary infection with this virus.
Subject(s)
Antibodies, Blocking/blood , Antibodies, Viral/blood , Coxsackievirus Infections/immunology , Coxsackievirus Infections/virology , Enterovirus B, Human/immunology , Enterovirus B, Human/physiology , Animals , Antibodies, Neutralizing/blood , Cell Line , Coxsackievirus Infections/blood , Coxsackievirus Infections/physiopathology , Enterovirus B, Human/pathogenicity , Host-Pathogen Interactions , Humans , Hyperglycemia , Mice , Neutralization Tests , Pancreas/pathology , Pancreas/virology , RNA, Viral , Spleen/pathology , Spleen/virology , Viral LoadABSTRACT
Autophagy is an intrinsic cellular process that can degrade cytoplasmic components. It has been reported that several pathogens hijack this process to facilitate their replication. Coxsackievirus B3 (CVB3), a member of the family Picornaviridae, induces autophagy upon infection. However, the details of CVB3-induced autophagy remain a subject of debate. This study applied a combination of multiple assays for the measurement of autophagy and demonstrated that CVB3 induces a complete autophagic flux. Experiments with infected HEK293A cells revealed that autophagosomes were induced upon CVB3 infection. Most of these autophagosomes were mCherry positive in mCherry-GFP-LC3 cells. Conversely, mCherry-positive autophagosomes were rescued to green positive when treated with the acidification inhibitors chloroquine (CQ) and bafilomycin A1 (BAF), suggesting that autophagosomes fused with late endosomes or lysosomes. The co-localization of LC3-positive puncta with lysosome-associated membrane protein 1 (LAMP1) or LysoTracker confirmed that the autophagosomes fused primarily with lysosomes. Interestingly, the disruption of autophagosome formation by 3-methyladenine (3-MA) or ATG5 siRNA treatment during viral infection significantly decreased CVB3 replication. However, inhibitors of lysosomal acidification, fusion, or degradation did not affect viral replication. Therefore, autolysosomes may not be critical for viral replication in vitro.
Subject(s)
Autophagosomes/virology , Autophagy , Coxsackievirus Infections/physiopathology , Enterovirus B, Human/physiology , Virus Replication , Autophagosomes/metabolism , Coxsackievirus Infections/genetics , Coxsackievirus Infections/metabolism , Coxsackievirus Infections/virology , Enterovirus B, Human/genetics , HEK293 Cells , Humans , Lysosomal-Associated Membrane Protein 1/genetics , Lysosomal-Associated Membrane Protein 1/metabolism , Lysosomes/metabolism , Lysosomes/virologyABSTRACT
Unilateral acute idiopathic maculopathy (UAIM) is a rare disorder presenting in young people with an acute onset of unilateral central visual loss often associated with a prodromal flu-like illness. The authors present the early anatomical findings of a 35-year-old man clinically diagnosed with UAIM using swept-source optical coherence tomography (SS-OCT) and SS-OCT angiography.
Subject(s)
Coxsackievirus Infections/diagnosis , Enterovirus/isolation & purification , Eye Infections, Viral/diagnosis , Retinal Detachment/diagnosis , Retinal Photoreceptor Cell Outer Segment/pathology , Retinal Pigment Epithelium/pathology , Acute Disease , Adult , Antibodies, Viral/blood , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/virology , Enterovirus/immunology , Eye Infections, Viral/physiopathology , Eye Infections, Viral/virology , Fluorescein Angiography , Humans , Immunoglobulin M/blood , Male , Retinal Detachment/physiopathology , Retinal Detachment/virology , Tomography, Optical Coherence , Visual Acuity/physiologySubject(s)
Coxsackievirus Infections/virology , Enterovirus B, Human/isolation & purification , Eye Infections, Viral/virology , Retinal Detachment/virology , Retinal Hemorrhage/virology , Skin Diseases, Viral/virology , Adult , Antibodies, Viral/blood , Blindness/diagnosis , Blindness/physiopathology , Blindness/virology , Coxsackievirus Infections/diagnosis , Coxsackievirus Infections/physiopathology , Enterovirus B, Human/immunology , Exudates and Transudates , Eye Infections, Viral/diagnosis , Eye Infections, Viral/physiopathology , Humans , Male , Retinal Detachment/diagnosis , Retinal Detachment/physiopathology , Retinal Hemorrhage/diagnosis , Retinal Hemorrhage/physiopathology , Skin Diseases, Viral/diagnosis , Skin Diseases, Viral/physiopathology , Tomography, Optical Coherence , Visual AcuityABSTRACT
This study is to determine the therapeutic effects of Panax notoginseng saponins (PNSs) on coxsackievirus B3 (CVB3)-induced myocarditis, and whether cystathionine-γ-lyase (CSE)/hydrogen sulfide (H2S) pathway is involved. Mouse model of myocarditis was induced by CVB3 infection, and the mice were subjected to vehicle (saline) or drug treatments (sodium bisulfide (NaHS), propargylglycine (PAG), or PNSs). The results showed that there were inflammatory cell infiltrations, interstitial edemas, and elevated inflammatory cytokines, in CVB3-induced myocarditis. PAG administration increased, whereas NaHS treatment decreased the severity of the myocarditis. PNS treatment dramatically alleviated these myocardial injuries and decreased the viral messenger RNA (mRNA) expression by the enhanced expression of CSE/H2S pathway. Moreover, the therapeutic effects of PNSs on myocarditis were stronger than those of NaHS. Finally, the effect of PNSs on CSE/H2S pathway and cardiac cell protection were verified in cultured cardiac cells. PNSs may be a promising medication for viral myocarditis therapy.
Subject(s)
Cystathionine gamma-Lyase/metabolism , Hydrogen Sulfide/metabolism , Myocarditis/drug therapy , Myocarditis/virology , Panax notoginseng , Plant Preparations/administration & dosage , Analysis of Variance , Animals , Biomarkers/metabolism , Blotting, Western , Coxsackievirus Infections/drug therapy , Coxsackievirus Infections/physiopathology , Disease Models, Animal , Enterovirus B, Human/drug effects , Enterovirus B, Human/isolation & purification , Enzyme-Linked Immunosorbent Assay , Humans , Male , Mice , Mice, Inbred BALB C , Phytotherapy/methods , RNA, Small Interfering/metabolism , Random Allocation , Real-Time Polymerase Chain Reaction/methods , Saponins/therapeutic use , Signal TransductionSubject(s)
Analgesics/administration & dosage , Antipyretics/administration & dosage , Coxsackievirus Infections , Enterovirus/isolation & purification , Hand, Foot and Mouth Disease , Acute Disease , Adult , Coxsackievirus Infections/diagnosis , Coxsackievirus Infections/etiology , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/therapy , Exanthema , Female , Fluid Therapy/methods , Hand, Foot and Mouth Disease/diagnosis , Hand, Foot and Mouth Disease/etiology , Hand, Foot and Mouth Disease/physiopathology , Hand, Foot and Mouth Disease/therapy , Humans , PrognosisABSTRACT
BACKGROUND: This study tested the hypothesis whether endoplasmic reticulum (ER) stress/C/EBP homologous protein (CHOP) signaling is linked with coxsackievirus B3 (CVB3)-induced acute viral myocarditis (AVMC) in vivo. METHODS AND RESULTS: AVMC was induced by intraperitoneal injection of 1000 tissue culture infectious dose (TCID50) of CVB3 virus in mice. In AVMC mouse hearts (n=11), ER stress and CHOP were significantly activated, and were linked to the induction of proapoptotic signaling including reduction of Bcl-2, activation of Bax and caspase 3, compared with the controls (n=10), whereas these could be markedly blocked by ER stress inhibitor tauroursodeoxycholic acid administration (n=11). Moreover, chemical inhibition of ER stress significantly attenuated cardiomyocytes apoptosis, and prevented cardiac troponin I elevation, ameliorated cardiac dysfunction assessed by both hemodynamic and echocardiographic analysis, reduced viral replication, and increased survival rate after CVB3 inoculation. We further discovered that genetic ablation of CHOP (n=10) suppressed cardiac Bcl-2/Bax ratio reduction and caspase 3 activation, and prevented cardiomyotes apoptosis in vivo, compared with wild-type receiving CVB3 inoculation (n=10). Strikingly, CHOP deficiency exhibited dramatic protective effects on cardiac damage, cardiac dysfunction, viral replication, and promoted survival in CVB3-caused AVMC. CONCLUSIONS: Our data imply the involvement of ER stress/CHOP signaling in CVB3-induced AVMC via proapoptotic pathways, and provide a novel strategy for AVMC treatment.
Subject(s)
Coxsackievirus Infections/metabolism , Endoplasmic Reticulum Stress , Endoplasmic Reticulum/metabolism , Enterovirus B, Human/pathogenicity , Myocarditis/metabolism , Myocytes, Cardiac/metabolism , Transcription Factor CHOP/metabolism , Animals , Antiviral Agents/pharmacology , Apoptosis , Caspase 3/metabolism , Coxsackievirus Infections/genetics , Coxsackievirus Infections/pathology , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/prevention & control , Coxsackievirus Infections/virology , Disease Models, Animal , Endoplasmic Reticulum/drug effects , Endoplasmic Reticulum/virology , Endoplasmic Reticulum Stress/drug effects , Enterovirus B, Human/drug effects , Enterovirus B, Human/growth & development , Male , Mice, Inbred C57BL , Mice, Knockout , Myocarditis/genetics , Myocarditis/pathology , Myocarditis/physiopathology , Myocarditis/prevention & control , Myocarditis/virology , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/pathology , Myocytes, Cardiac/virology , Proto-Oncogene Proteins c-bcl-2/metabolism , Signal Transduction , Taurochenodeoxycholic Acid/pharmacology , Transcription Factor CHOP/deficiency , Transcription Factor CHOP/genetics , Virus Replication/drug effects , bcl-2-Associated X Protein/metabolismABSTRACT
Myocarditis is an inflammatory disease caused by viral infection. Different subpopulations of leukocytes enter the cardiac tissue and lead to severe cardiac inflammation associated with myocyte loss and remodeling. Here, we study possible cell sources for viral replication using three compartments of the heart: fibroblasts, cardiomyocytes, and macrophages. We infected C57BL/6j mice with Coxsackievirus B3 (CVB3) and detected increased gene expression of anti-inflammatory and antiviral cytokines in the heart. Subsequently, we infected cardiac fibroblasts, cardiomyocytes, and macrophages with CVB3. Due to viral infection, the expression of TNF-α, IL-6, MCP-1, and IFN-ß was significantly increased in cardiac fibroblasts compared to cardiomyocytes or macrophages. We found that in addition to cardiomyocytes cardiac fibroblasts were infected by CVB3 and displayed a higher virus replication (132-fold increase) compared to cardiomyocytes (14-fold increase) between 6 and 24 hours after infection. At higher virus concentrations, macrophages are able to reduce the viral copy number. At low virus concentration a persistent virus infection was determined. Therefore, we suggest that cardiac fibroblasts play an important role in the pathology of CVB3-induced myocarditis and are another important contributor of virus replication aggravating myocarditis.
Subject(s)
Coxsackievirus Infections/pathology , Coxsackievirus Infections/virology , Enterovirus B, Human/physiology , Enterovirus B, Human/pathogenicity , Myocarditis/pathology , Myocarditis/virology , Myocardium/pathology , Animals , Cells, Cultured , Coxsackievirus Infections/physiopathology , Cytokines/genetics , Enterovirus B, Human/genetics , Fibroblasts/immunology , Fibroblasts/pathology , Fibroblasts/virology , Gene Expression , Genome, Viral , Heart/virology , Macrophages/immunology , Macrophages/pathology , Macrophages/virology , Male , Mice , Mice, Inbred C57BL , Myocarditis/physiopathology , Myocardium/immunology , Myocytes, Cardiac/immunology , Myocytes, Cardiac/pathology , Myocytes, Cardiac/virology , Ventricular Function, Left , Viral Load , Virus ReplicationABSTRACT
OBJECTIVE: To investigate the correlations among persistent viral infection, heart function and Chinese medicine (CM) difined-syndromes in patients with dilated cardiomyopathy (DCM). METHODS: Fifty patients with DCM in the First Affiliated Hospital of Zhejiang Chinese Medical University from October 2009 to December 2011 were selected as the research subjects, and 30 healthy people were simultaneously selected as the normal control group to detect persistent viral infections after admission. The CM syndrome type and grade of heart function were then evaluated. The expression level of Coxsackie adenovirus receptor (CAR) was detected using the flow cytometry (FCM) technique, coxsackie virus RNA (CVB-RNA) using reverse transcription polymerase chain reaction (RTPCR), and the plasma brain natriuretic peptide (BNP) level with a Triage meter plus diagnosis instrument. Finally, the parameters such as left ventricular end diastolic diameter (LVEDd) and left ventricular ejection fraction (LVEF) were measured by ultrasonic cardiogram. Person correlation analysis was used for measured data, Spearman correlation analysis for rating data, and the Chi-square test for numerical data. RESULTS: CVB-RNA was positive in 22 patients (44%) with DCM, while only 6 cases (20%) were CVB-RNA-positive in the normal control group, with a significant difference between the two groups (P<0.01). The expression level of CAR was significantly elevated in the DCM group compared with the normal control group (P<0.01). In CVB-RNA-positive patients (22 cases), the expression level of CAR was significantly higher than in CVB-RNA-negative patients (28 cases; P<0.01). In the DCM patients, there was a positive correlation between the CAR expression and the BNP level (r=0.34, P<0.05), while no significant difference was found between the CAR expression and the LVEF and LVEDd (r=-0.32, 0.30, P>0.05). There was no clear correlation between virus infection and the CM syndrome types in DCM patients (r=-0.22, P>0.05). According to the sequence of syndrome types: phlegm â qi deficiency â blood stasis â hydroretention with asthenic yang (from low to high), a positive correlation was existed between the BNP levels and CM syndrome types (r=0.139, P<0.05). CONCLUSION: The expression of CAR on the surface of white cells could be used to detect persistent viral infection. The expression level of CAR and heart function in DCM patients were highly correlated. The expression level of BNP may serve as an objective index for differentiating CM syndromes for patients with DCM.
Subject(s)
Cardiomyopathy, Dilated/complications , Cardiomyopathy, Dilated/physiopathology , Coxsackievirus Infections/complications , Coxsackievirus Infections/physiopathology , Heart Function Tests , Medicine, Chinese Traditional , Adult , Aged , Cardiomyopathy, Dilated/blood , Cardiomyopathy, Dilated/virology , Coxsackie and Adenovirus Receptor-Like Membrane Protein , Coxsackievirus Infections/blood , Female , Humans , Male , Middle Aged , Natriuretic Peptide, Brain/blood , RNA, Viral/blood , SyndromeABSTRACT
Adiponectin (APN) is an immunomodulatory adipocytokine that improves outcome in patients with virus-negative inflammatory cardiomyopathy and mice with autoimmune myocarditis. Here, we investigated whether APN modulates cardiac inflammation and injury in coxsackievirus B3 (CVB3) myocarditis. Myocarditis was induced by CVB3 infection of APN-KO and WT mice. APN reconstitution was performed by adenoviral gene transfer. Expression analyses were performed by qRT-PCR and immunoblot. Cardiac histology was analyzed by H&E-stain and immunohistochemistry. APN-KO mice exhibited diminished subacute myocarditis with reduced viral load, attenuated inflammatory infiltrates determined by NKp46, F4/80 and CD3/CD4/CD8 expression and reduced IFNß, IFNγ, TNFα, IL-1ß and IL-12 levels. Moreover, myocardial injury assessed by necrotic lesions and troponin I release was attenuated resulting in preserved left ventricular function. Those changes were reversed by APN reconstitution. APN had no influence on adhesion, uptake or replication of CVB3 in cardiac myocytes. In acute CVB3 myocarditis, cardiac viral load did not differ between APN-KO and WT mice. However, APN-KO mice displayed an enhanced acute immune response, i.e. increased expression of myocardial CD14, IFNß, IFNγ, IL-12, and TNFα resulting in increased cardiac infiltration with pro-inflammatory M1 macrophages and activated NK cells. Up-regulation of cardiac CD14 expression, type I and II IFNs and inflammatory cell accumulation in APN-KO mice was inhibited by APN reconstitution. Our observations indicate that APN promotes CVB3 myocarditis by suppression of toll-like receptor-dependent innate immune responses, polarization of anti-inflammatory M2 macrophages and reduction of number and activation of NK cells resulting in attenuated acute anti-viral immune responses.
Subject(s)
Adiponectin/metabolism , Coxsackievirus Infections/metabolism , Enterovirus B, Human/immunology , Myocarditis/metabolism , Myocardium/metabolism , Adiponectin/deficiency , Adiponectin/genetics , Animals , Animals, Newborn , Cells, Cultured , Coxsackievirus Infections/genetics , Coxsackievirus Infections/immunology , Coxsackievirus Infections/pathology , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/virology , Disease Models, Animal , Enterovirus B, Human/genetics , Enterovirus B, Human/pathogenicity , Immunity, Innate , Inflammation Mediators/metabolism , Killer Cells, Natural/immunology , Killer Cells, Natural/metabolism , Killer Cells, Natural/virology , Macrophages/immunology , Macrophages/metabolism , Macrophages/virology , Mice , Mice, Inbred C57BL , Mice, Knockout , Myocarditis/genetics , Myocarditis/immunology , Myocarditis/pathology , Myocarditis/physiopathology , Myocarditis/virology , Myocardium/immunology , Myocardium/pathology , Necrosis , Rats , Rats, Wistar , Toll-Like Receptors/metabolism , Ventricular Function, Left , Viral LoadABSTRACT
INTRODUCTION: Myocarditis is induced by coxsackievirus B3 (CVB3). Myocardial inflammation is tied to the activation of coagulation. Coagulation factor (F) Xa, a central player in coagulation, activates matrix metalloproteinases (MMP), which modulate the remodeling. AIMS: In this study, we investigated the effects of pharmacological FXa inhibition on myocardial function, inflammation, and remodeling during a CVB3-induced myocarditis. METHODS AND RESULTS: Immune cells and matrix proteins were detected by immunohistochemistry. The expression of cytokines was measured by real-time PCR and the activity of MMP-2 by zymography. Left ventricular function was analyzed using microconductance pressure catheter. Treatment with the FXa inhibitor fondaparinux led to an improved left ventricular function in CVB3-induced mice compared to saline-treated controls (dPdtmax: fondaparinux 4632 ± 499.6 vs. saline 3131 ± 374.0 [mmHg/s], P = 0.0503; SV: fondaparinux 33.19 ± 4.893 vs. saline 19.32 ± 2.236 [µL], P < 0.118; CO: fondaparinux 15124 ± 2183 vs. saline 8088 ± 1035 [µL/min], P < 0.05). Therapy with fondaparinux reduced the activity of MMP-2 (fondaparinux 1.208 ± 0.1247 vs. saline 1.565 ± 0.05476, P < 0.05). The collagen type I/III ratio as well as the expression of TIMP-1 was comparable in both infection groups postinfectionem (p.i.), despite an increased infiltration of macrophages into the hearts of mice treated with fondaparinux 8 days p.i. (CD68+: fondaparinux 494.2 ± 64.73 vs. saline 306.9 ± 43.73 [cells/mm(2) ], P < 0.05). Anti-inflammatory CD206-positive M2-type macrophages were increased in the infected hearts after fondaparinux treatment (CD206+: fondaparinux 182.1 ± 18.18 vs. saline 111.6 ± 21.07 [cells/mm(2) ], P < 0.05), whereas CD80-positive M1-type macrophages were comparable in both groups. CONCLUSION: In conclusion, selective inhibition of FXa improves the left ventricular function during CVB3-induced myocarditis and seems to be associated with an improved myocardial remodeling.
Subject(s)
Coxsackievirus Infections/drug therapy , Enterovirus B, Human/pathogenicity , Factor Xa Inhibitors/pharmacology , Factor Xa/metabolism , Myocarditis/drug therapy , Polysaccharides/pharmacology , Ventricular Function, Left/drug effects , Ventricular Remodeling/drug effects , Animals , Coxsackievirus Infections/blood , Coxsackievirus Infections/physiopathology , Coxsackievirus Infections/virology , Cytokines/genetics , Cytokines/metabolism , Disease Models, Animal , Fondaparinux , Inflammation Mediators/metabolism , Male , Matrix Metalloproteinase 2/metabolism , Mice, Inbred C57BL , Myocarditis/blood , Myocarditis/physiopathology , Myocarditis/virology , RNA, Messenger/metabolism , Viral LoadABSTRACT
Calpain is a family of neutral cysteine proteinase involved in many physiological and pathological processes including virus replication, autophagy and apoptosis. Previous study has indicated the involvement of calpain in pathogenesis of coxsackievirus B3 (CVB3)-induced myocarditis. Besides, many studies demonstrated that host cell autophagy and apoptosis mechanisms participate in virus life cycle. However, role of calpain in CVB3 replication via autophagy/apoptosis mechanisms has not been reported, which was discussed here in H9c2 cardiomyocytes. The data demonstrated that calpain was activated following CVB3 infection. Calpain inhibition decreased autophagy, indicating role of calpain in enhancing autophagy during CVB3 infection. Both calpain activity and autophagy were involved in facilitating CVB3 replication demonstrated by virus titer and CVB3 capsid protein VP1 expression alterations resulting from calpain inhibitor ALLN and autophagy inhibitor 3MA intervention. We also found that both calpain activity and autophagy suppressed caspase3 activity and host cell apoptosis 5-10h post-infection (p.i.). In summary, the present study shows that CVB3 infection of H9c2 cells hinders caspase3 activity provocation and cell apoptosis at least in the early phase of infection (5-10h p.i.) via calpain-induced autophagy enhancement, which might be a mechanism facilitating CVB3 replication in host cells.