ABSTRACT
Streptococcus pneumoniae (pneumococcal) meningitis is a common bacterial infection of the brain. The cholesterol-dependent cytolysin pneumolysin represents a key factor, determining the neuropathogenic potential of the pneumococci. Here, we demonstrate selective synaptic loss within the superficial layers of the frontal neocortex of post-mortem brain samples from individuals with pneumococcal meningitis. A similar effect was observed in mice with pneumococcal meningitis only when the bacteria expressed the pore-forming cholesterol-dependent cytolysin pneumolysin. Exposure of acute mouse brain slices to only pore-competent pneumolysin at disease-relevant, non-lytic concentrations caused permanent dendritic swelling, dendritic spine elimination and synaptic loss. The NMDA glutamate receptor antagonists MK801 and D-AP5 reduced this pathology. Pneumolysin increased glutamate levels within the mouse brain slices. In mouse astrocytes, pneumolysin initiated the release of glutamate in a calcium-dependent manner. We propose that pneumolysin plays a significant synapto- and dendritotoxic role in pneumococcal meningitis by initiating glutamate release from astrocytes, leading to subsequent glutamate-dependent synaptic damage. We outline for the first time the occurrence of synaptic pathology in pneumococcal meningitis and demonstrate that a bacterial cytolysin can dysregulate the control of glutamate in the brain, inducing excitotoxic damage.
Subject(s)
Frontal Lobe/metabolism , Glutamic Acid/metabolism , Meningitis, Pneumococcal/metabolism , Receptors, N-Methyl-D-Aspartate/metabolism , Streptococcus pneumoniae/metabolism , Streptolysins/metabolism , Synapses/metabolism , Adult , Aged , Aged, 80 and over , Animals , Astrocytes/metabolism , Astrocytes/microbiology , Astrocytes/pathology , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Dendrites/metabolism , Dendrites/microbiology , Dendrites/pathology , Dizocilpine Maleate/pharmacology , Frontal Lobe/microbiology , Frontal Lobe/pathology , Humans , Meningitis, Pneumococcal/genetics , Meningitis, Pneumococcal/microbiology , Meningitis, Pneumococcal/pathology , Mice , Middle Aged , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Streptococcus pneumoniae/genetics , Streptococcus pneumoniae/pathogenicity , Streptolysins/genetics , Synapses/microbiology , Synapses/pathologyABSTRACT
It has recently been shown that bacteria and viruses can be delivered to the brain parenchyma without evoking an immune response. These experiments demonstrate that there are no cells within the brain parenchyma that can initiate a primary immune response, and that the drainage of pathogens from the brain parenchyma is distinct from that documented for soluble proteins. A persistent pathogen in the brain parenchyma can become a target for the immune system following peripheral sensitisation, and this may lead to bystander tissue damage. These observations may have consequences for vaccination of persons with central nervous system HIV infection.
Subject(s)
Brain/immunology , Brain/microbiology , Hypersensitivity, Delayed/immunology , Animals , BCG Vaccine/pharmacology , Blood-Brain Barrier , Brain/pathology , Dendrites/microbiology , Dendrites/pathology , Hypersensitivity, Delayed/pathology , Mycobacterium bovis , Rats , Vaccines, Inactivated/pharmacologyABSTRACT
The sequential distribution of transneuronally infected neurons was studied in the olfactory pathway of mice after unilateral inoculation of the challenge virus standard (CVS) strain in the nasal cavity. A first cycle of viral multiplication was observed in a subpopulation of receptor cells scattered in the main olfactory epithelium and in the septal organ. No viral spread from cell body to cell body was reported even in later stages of infection. The second round of viral replication which took place in the ipsilateral main olfactory bulb at 2 and 2.5 days post-inoculation (p.i.), involved second order neurons and periglomerular cells, known to be directly connected with the axon terminals of receptor cells. Also reported as a result of a second cycle of viral replication, was surprisingly the spread of CVS at 2 and 2.5 days p.i. in bulbar interneurons located in the internal plexiform layer and in the superficial granule cell layer, as well as that of 2 ipsilateral cerebral nuclei, the anterior olfactory nucleus and the horizontal limb of the diagonal band. From day 3, a rapid spread of CVS was suggested by detection of virus in all ipsilateral direct terminal regions of the second order neurons and in most tertiary olfactory projections. The locus coeruleus, a noradrenergic nucleus which sends direct afferents to the olfactory bulb, never appeared immunoreactive. In spite of a certain inability of CVS to infect some neuron types, the virus appears relevant to provide new information regarding the complex network of olfactory-related neurons into the CNS.
Subject(s)
Afferent Pathways/microbiology , Brain/microbiology , Neurons/microbiology , Olfactory Bulb/microbiology , Olfactory Pathways/microbiology , Rabies virus/pathogenicity , Virus Replication , Afferent Pathways/anatomy & histology , Afferent Pathways/pathology , Animals , Axonal Transport , Brain/anatomy & histology , Brain/pathology , Dendrites/microbiology , Dendrites/ultrastructure , Epithelium/microbiology , Epithelium/pathology , Female , Fluorescent Antibody Technique , Golgi Apparatus/microbiology , Golgi Apparatus/ultrastructure , Mice , Mice, Inbred Strains , Neurons/pathology , Olfactory Bulb/anatomy & histology , Olfactory Pathways/anatomy & histology , Organ Specificity , Rabies virus/physiology , Time Factors , VirulenceABSTRACT
Cultured hippocampal neurons were infected with a temperature-sensitive mutant of vesicular stomatitis virus (VSV) and a wild-type strain of the avian influenza fowl plague virus (FPV). The intracellular distribution of viral glycoproteins was monitored by immunofluorescence microscopy. In mature, fully polarized neurons the VSV glycoprotein (a basolateral protein in epithelial MDCK cells) moved from the Golgi complex to the dendritic domain, whereas the hemagglutinin protein of FPV (an apically sorted protein in MDCK cells) was targeted preferentially, but not exclusively, to the axon. The VSV glycoprotein appeared in clusters on the dendritic surface, while the hemagglutinin was distributed uniformly along the axonal membrane. Based on the finding that the same viral glycoproteins are sorted in a polarized fashion in both neuronal and epithelial cells, we propose that the molecular mechanisms of surface protein sorting share common features in the two cell types.
Subject(s)
Axons/microbiology , Dendrites/microbiology , Glycoproteins/analysis , Hippocampus/microbiology , Influenza A virus/genetics , Neurons/microbiology , Vesicular stomatitis Indiana virus/genetics , Viral Proteins/analysis , Animals , Cells, Cultured , Embryo, Mammalian , Fluorescent Antibody Technique , Glycoproteins/genetics , Rats , Viral Proteins/geneticsABSTRACT
Ultrastructural immunohistochemistry was used to localize type 2 human poliovirus (HPV 2) during virulent infection of mice caused by the Lansing strain. In the spinal cord, immune-reaction product was exclusively localized within neurons and their processes. The absence of viral antigen in glial, endothelial and inflammatory cells further supports the strict neuronotropicity of HPV. In addition, viral antigen and virus-like particles were localized in synaptic complexes and axons, including preterminal axons. This clear demonstration of HPV in neuronal cell bodies, their axons, and synaptic elements strongly supports the hypothesis of HPV dissemination in the central nervous system via axonal transport.
Subject(s)
Poliomyelitis/microbiology , Poliovirus/isolation & purification , Animals , Antigens, Viral/analysis , Dendrites/microbiology , Histocytochemistry , Immunochemistry , Male , Mice , Mice, Inbred Strains , Microscopy, Electron , Poliovirus/pathogenicity , Spinal Cord/microbiology , Spinal Cord/ultrastructure , VirulenceABSTRACT
The mechanism by which neurotropic arboviruses gain access to the central nervous system remains uncertain, although it is generally assumed that viremic infection results in growth across or passive diffusion through brain capillaries. In contrast to the natural reservoir hosts of these arboviruses, clinical hosts (e.g., horses, humans) have viremias of very brief duration and low magnitude. We investigated the question of neuroinvasion in 5- to 6-week-old Syrian hamsters infected with St. Louis encephalitis virus (strain TBH-28). This model shares with the human disease low or undetectable viremia and many clinical and pathoanatomical features. The mortality rate after intraperitoneal inoculation of a moderate viral dose was 88%. No viremia was detectable by a sensitive assay in 31% of the animals. In the remaining hamsters, the mean peak viremia was 1.0 log10 plaque-forming units/0.05 ml and the mean duration 1 to 2 days. There was no correlation between viremia and outcome of infection, length of incubation period, or brain virus titer. Tissue infectivity studies showed a rise in titer in the olfactory neuroepithelium on day 4 postinoculation, then in the olfactory bulbs (day 5 postinoculation), and finally in the remainder of the brain (day 6 postinoculation). Specific immunofluorescence was demonstrated in the bipolar neurons of the olfactory epithelium, their dendrites, and in axon bundles of the olfactory nerves in the submucosa. By electron microscopy, virus particles and associated tubular structures were demonstrated within dendrites, perikarya, and axons of olfactory neurons, and to a lesser extent in macrophages and Bowman's gland cells in the lamina propria. In cells of Bowman's glands large numbers of virions were sequestered within secretory granules. Virus was recovered from nasal washings on day 4 postinoculation. Similar findings were obtained in weanling mice inoculated intraperitoneally with another (mouse-virulent) St. Louis encephalitis viral strain (77V-12908). These data taken together indicate that the olfactory pathway is the principal route of viral entry into the central nervous system. After peripheral inoculation a low-level viremia results in infection of highly susceptible cells in the olfactory neuroepithelium, allowing centripetal axonal transport of virus to the olfactory bulb, whence spread is unimpeded throughout the neuropil of the central nervous system. Infection of Bowman's gland cells in the olfactory mucosa and shedding of virus in nasal mucus may be an adaptation for nonarthropod-borne transmission, a feature of many flaviviruses.
Subject(s)
Brain/microbiology , Encephalitis Virus, St. Louis/physiology , Encephalitis, St. Louis/microbiology , Flavivirus/physiology , Olfactory Mucosa/microbiology , Animals , Axons/microbiology , Cricetinae , Dendrites/microbiology , Exocrine Glands/microbiology , Female , Male , Mesocricetus , Mice , Neurons/microbiology , Olfactory Bulb/microbiology , Olfactory Mucosa/innervation , Olfactory Nerve/microbiology , ViremiaABSTRACT
Within the nervous system the highly specialized structure and function of nerve cells renders the pathogenesis of viral infections amazingly complex. In vivo and in vitro studies reveal that viruses may display tropism for distinct types of cells such as neurons, myelin-forming cells, or astrocytes. In neurons, RNA viruses mature in the cell body and in dendrites close to synapses, from which they can spread to synaptic endings. Undefined host factors and stage of differentiation may favor defective viral assembly, which, in turn, results in persistent infections of neurons. In myelin-forming cells, lytic infection results is persistent infections of neurons. In myelin-forming cells, lytic infection results in degeneration of myelin and, consequently, in altered conduction in those axons that are ensheathed by a myelin-forming cell. In addition, breakdown of myelin may induce an autoimmune response, which then leads to further demyelination. Autoimmune demyelination may also occur when glial cells other than myelin-forming cells are infected. Astrocytes are prone to persistent infection or viral transformation.
Subject(s)
Neuroglia/microbiology , Neurons/microbiology , Schwann Cells/microbiology , Virus Diseases/microbiology , Virus Replication , Animals , Astrocytes/microbiology , Autoantibodies , Birds , Cells, Cultured , Demyelinating Diseases/microbiology , Dendrites/microbiology , Distemper/microbiology , Dogs , Herpes Simplex/microbiology , Humans , Marek Disease/microbiology , Oligodendroglia/microbiology , Synapses/microbiology , Time FactorsABSTRACT
The paper systematizes the data of the literature on ultrastructural changes in the central nervous system (CNS) of experimental animals infected with arboviruses of the Togaviridae family. The main site of virus reproduction in the CNS was found to be nerve cells in which the ultrastructural lesions typical for this group of viruses developed. The main features of these lesions consist in degeneration of rough membranes, ribosomes, and polysomes of the cell, hyperplasia and hypertrophy of smooth membranes, formation of various vacuoles and vesicles in the cell hyaloplasm. Mature virions accumulate in cisterns and cavities of the endoplasmic reticulum, lamellar complex, in vacuoles and vesicles. Each of arboviruses produces in the cells not strictly specific but typical ultrastructural lesions. In the CNS the viruses spread hematogenically, in intercellular and perivascular spaces and dendrites of the nerve cells.
Subject(s)
Arbovirus Infections/pathology , Cell Transformation, Viral , Central Nervous System Diseases/pathology , Central Nervous System/ultrastructure , Animals , Arbovirus Infections/microbiology , Central Nervous System/microbiology , Central Nervous System Diseases/microbiology , Dendrites/microbiology , Dendrites/ultrastructure , Encephalitis, Tick-Borne/microbiology , Encephalitis, Tick-Borne/pathology , Mice , Microscopy, Electron , Neuroglia/microbiology , Neuroglia/ultrastructure , Neurons/microbiology , Neurons/ultrastructure , Virus ReplicationABSTRACT
Electron microscopic investigation of the brains and lumbar spinal cords of adult albino mice infected with Powassan virus was carried out. Virus particles were found within all parts of neurons (perikarya, dendrites, axon), as well as within synaptic apparatus and intercellular gaps of the central nervous tissue. The possibility of the virus spread both throughout the cytoplasm of nerve cells and their processes and the extracellular spaces of the brain was confirmed. Localization of virions within neurons, synapses and myelinated fibers of the spinal cord after intracerebral inoculation suggests that virus spread in the CNS can occur through the CNS parenchyma and also through the nervous conduction pathways. The possible mechanisms of virus dissemination in the CNS of albino mice with experimental Powassan virus encephalomyelitis are discussed.
Subject(s)
Central Nervous System/microbiology , Encephalitis Viruses, Tick-Borne/pathogenicity , Encephalitis, Tick-Borne/microbiology , Neurons/microbiology , Animals , Axons/microbiology , Brain/microbiology , Dendrites/microbiology , Inclusion Bodies, Viral , Mice , Neural Pathways/microbiology , Spinal Cord/microbiology , Virion/pathogenicityABSTRACT
Two strains of tissue culture-grown rabies virus developed in suckling mouse brain predominantly by the process of virus budding from the neuronal cell membrane.
Subject(s)
Brain/microbiology , Cell Membrane/microbiology , Rabies virus/growth & development , Virus Replication , Animals , Cytoplasm/microbiology , Dendrites/microbiology , Hippocampus/microbiology , Mice , Mice, Inbred ICR , Neurons/microbiologyABSTRACT
A previously healthy middle aged man died following a 6 month illness which presented with middle ear symptoms, apparently resolved, and then 2 months later manifested as encephalitis. The illness was characterized initially by depression and intellectual deterioration. No family member or working associate was affected. The clinical diagnosis of viral encephalitis was confirmed by brain biopsy but no virus was isolated in the laboratory. Numerous intracisternal toroidal virus-like particles were demonstrated by electron microscopy in the perikarya and dendrites but not in glia. The particles resemble, but are not identical to, the oncornaviruses associated with spontaneous and induced murine neoplasms. The resemblance of these structures to the intracisternal toroidal type "A" virus of murine leukemia is noted and other possible causes for this atypical meningoencephalitis are discussed.
Subject(s)
Inclusion Bodies, Viral , Meningoencephalitis/microbiology , Dendrites/microbiology , Endoplasmic Reticulum/microbiology , Helper Viruses/analysis , Humans , Lymphocytic choriomeningitis virus , Male , Meningoencephalitis/pathology , Microscopy, Electron , Middle Aged , Neuroglia/microbiology , Neurons/microbiology , Oncogenic Viruses/analysis , RNA Viruses/analysisABSTRACT
In experimental yellow fever virus encephalomyelitis of adult albino mice, virions, and virus-associated structures were observed not only inside neuronal perikarya but also within dendrites of varied size. The finding permits the following explanations: (1) either the viral agent is synthesized in the nerve cell bodies and transported intradendritically in a proximodistal direction; or (2) virus morphogenesis takes place in neuronal perikarya and dendrites as well; or (3) both possiblities are equally valid. Some incidental findings were suggestive of virus release at postsynaptic dendrite membranes. They are discussed with reference to a hypothetical long-distance pathway of viral dissemination involving endocytosis of the agent by presynaptic axon terminals, intraaxonal virus decoating, and retrograde axoplasmic transport of the infectious nucleic acid to the cell soma.
Subject(s)
Central Nervous System/microbiology , Dendrites/microbiology , Encephalomyelitis/microbiology , Yellow Fever/microbiology , Animals , Central Nervous System/ultrastructure , Encephalomyelitis/pathology , Inclusion Bodies, Viral/ultrastructure , Mice , Synapses/microbiology , Synapses/ultrastructure , Yellow Fever/pathology , Yellow fever virus/ultrastructureABSTRACT
Comparative ultrastructural studies were performed on the development of Junín virus in mouse brain and in cerebellum explants and brain monolayers of the same animal. In mouse brain, neurons and astrocytes released virus particles by a budding mechanism identical to that previously described for this virus. In the neurons, the viral multiplication took place in the perikarion as well as in the cytoplasmic processes, including areas near synapses. Viral particles were observed emerging from pericapillary neurons and astrocytes. In the explants, the budding also occurred in neurons and astrocytes. In the monolayers, however, the virus originated in astrocytes and cells of fibroblastic appearance, which were the two cell types that developed in this substrate. These results indicate that the characteristics of the development of Junín virus in mouse brain are faithfully reproduced in cerebellum explants from the same animal, thus allowing some extrapolation of data from one system to the other. The explant proved to be a better model than the monolayer, not only because it reproduced the structural complexity of nervous tissue better, but also because it contains neurons and astrocytes, i.e., the two cell types that release the virus in the in vivo system.