ABSTRACT
The search for new methods of antiviral therapy is primarily focused on the use of substances of natural origin. In this context, a triterpene compound, betulin 1, proved to be a good starting point for derivatization. Thirty-eight betulin acid ester derivatives were synthetized, characterized, and tested against DNA and RNA viruses. Several compounds exhibited 4- to 11-fold better activity against Enterovirus E (compound 5 EC50: 10.3 µM) and 3- to 6-fold better activity against Human alphaherpesvirus 1 (HHV-1; compound 3c EC50: 17.2 µM). Time-of-addition experiments showed that most of the active compounds acted in the later steps of the virus replication cycle (e.g., nucleic acid/protein synthesis). Further in-silico analysis confirmed in-vitro data and demonstrated that interactions between HHV-1 DNA polymerase and the most active compound, 3c, were more stable than interactions with the parent non-active betulin 1.
Subject(s)
Antiviral Agents/pharmacology , Dicarboxylic Acids/pharmacology , Drug Design , Esters/pharmacology , Triterpenes/pharmacology , Antiviral Agents/chemical synthesis , Antiviral Agents/chemistry , DNA Viruses/drug effects , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/chemistry , Dose-Response Relationship, Drug , Esters/chemical synthesis , Esters/chemistry , Microbial Sensitivity Tests , Molecular Structure , RNA Viruses/drug effects , Structure-Activity Relationship , Triterpenes/chemical synthesis , Triterpenes/chemistryABSTRACT
The TET (Ten-Eleven Translocation) dioxygenase enzyme family comprising 3 members, TET1-3, play key roles in DNA demethylation. These processes regulate transcription programs that determine cell lineage, survival, proliferation, and differentiation. The impetus for our investigations described here is derived from the need to develop illuminating small molecule probes for TET enzymes with cellular activity and specificity. The studies were done so in the context of the importance of TET2 in the hematopoietic system and the preponderance of loss of function somatic TET2 mutations in myeloid diseases. We have identified that 2-hydroxy-4-methylene-pentanedicarboxylic acid 2a reversibly competes with the co-substrate α-KG in the TET2 catalytic domain and inhibits the dioxygenase activity with an IC50 = 11.0 ± 0.9 µM at 10 µM α-KG in a cell free system and binds in the TET2 catalytic domain with Kd = 0.3 ± 0.12 µM.
Subject(s)
Catalytic Domain/drug effects , DNA-Binding Proteins/metabolism , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/pharmacology , Dioxygenases/metabolism , Cell-Free System , DNA Methylation , Dicarboxylic Acids/chemistry , Humans , Molecular Docking Simulation , Spectrum Analysis/methods , Structure-Activity Relationship , THP-1 CellsABSTRACT
A series of coumarin-like diacid derivatives were designed and synthesized as novel agonists of human G-protein-coupled receptor 35 (hGPR35). Active compounds were characterized to possess one acidic group on both sides of a fused tricyclic aromatic scaffold. Most of them functioned as full agonists selective to hGPR35 and exhibited excellent potency at low nanomolar concentrations. Substitution on the middle ring of the scaffold could effectively regulate compound potency. Structure-activity relationship studies and docking simulation indicated that compounds that carried two acidic groups with a proper special distance and attached to a rigid aromatic scaffold would most likely show a potent agonistic activity on hGPR35. Following this principle, we screened a list of known compounds and some were found to be potent GPR35 agonists, and compound 24 even had an EC50 of 8 nM. Particularly, a dietary supplement pyrroloquinoline quinone (PQQ) was identified as a potent agonist (EC50 = 71.4 nM). To some extent, this principle provides a general strategy to design and recognize GPR35 agonists.
Subject(s)
Coumarins/pharmacology , Dicarboxylic Acids/pharmacology , Receptors, G-Protein-Coupled/agonists , Animals , CHO Cells , Catalytic Domain , Cell Line, Tumor , Coumarins/chemical synthesis , Coumarins/metabolism , Cricetulus , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/metabolism , Drug Design , Humans , Molecular Docking Simulation , Molecular Structure , Receptors, G-Protein-Coupled/metabolism , Structure-Activity RelationshipABSTRACT
A silica based hydrophilic stationary phase bonding with 2,3-pyrazinedicarboxylic anhydride and amino groups was synthesized via amino-acid anhydride ring opening reaction. The bonded groups could not only provide hydrophilic interaction, but also electrostatic, π-π and hydrogen bonding interactions, etc. The results of characterization with elemental analysis and solid-state 13C cross-polarization magic-angle-spinning NMR indicated the successful preparation of amino and carboxyl bonded stationary phase named ZAC. The ζ-potential of ZAC stationary phase showed the negatively charge was dominate at pH larger than 3.5. Chromatographic evaluation revealed that ZAC stationary phase behaved well under HILIC mode. It showed different selectivity and retention compared to some typical commercial columns, and it was validated by the separation of chitooligosaccharides, flavonoid glycosides, organic acids and alkaloid samples. Based on the different selectivity between ZAC stationary phase and C18 columns, ZAC stationary phase also showed different selectivity with C18. And it was verified by the separation of Lonicerae Japonicae Flos and Menispermi Rhizoma extracts.
Subject(s)
Anhydrides/chemistry , Chromatography/methods , Dicarboxylic Acids/chemistry , Pyrazines/chemistry , Alkaloids/isolation & purification , Dicarboxylic Acids/chemical synthesis , Flavonoids/isolation & purification , Hydrophobic and Hydrophilic Interactions , Magnetic Resonance Spectroscopy , Oligosaccharides/chemistry , Pyrazines/chemical synthesis , Silicon Dioxide/chemistry , Water/chemistryABSTRACT
Nb(0.05 moles%)-zeolites prepared via a post synthesis methodology (BEA, Y, ZSM-5), or a direct sol-gel method (Silicalite-1) were investigated in the hydroxymethylfurfural (HMF) oxidation by both molecular oxygen, in aqueous phase, and organic peroxides, in acetonitrile. The catalysts prepared through the post synthesis methodology (i.e., Nb-Y5, Nb-ZSM25, Nb-Y30, Nb-BEA12, and Nb-BEA18) displayed a mono-modal mesoporosity and contain residual framework Al-acid sites, extra framework isolated Nb(V)O-H and Nb2O5 pore-encapsulated clusters, while Nb-Sil-1, prepared through a direct synthesis procedure, displayed a bimodal micro-mesoporosity and contains only -Nb=O species. These modified zeolites behave as efficient catalysts in both HMF/glucose wet oxidation to succinic acid (SA) and HMF oxidation with organic peroxides to the 2,5-furandicarboxylic acid (FDCA). The catalytic behavior of these catalysts, in terms of conversion and especially the selectivity, mainly depended on the base/acid sites ratio. Thus, the HMF/glucose wet oxidation occurred with a total conversion and a selectivity to SA of 37.7% (from HMF) or 69.1% (from glucose) on the Nb-Y5 catalyst, i.e., the one with the lowest base/acid sites ratio. On the contrary, the catalysts with the highest base/acid sites ratio, i.e., Nb-ZSM25 and Nb-Sil-1, afforded a high catalytic efficiency in HMF oxidation with organic peroxides, in which FDCA was produced with selectivities of 61.3-63.8% for an HMF conversion of 96.7-99.0%.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Furans/chemical synthesis , Niobium/chemistry , Oxides/chemistry , Succinic Acid/chemical synthesis , Zeolites/chemistry , Adsorption , Catalysis , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , Glucose/chemistry , Nitrogen/chemistry , Oxidation-Reduction , Oxygen/chemistry , Peroxides/chemistry , PorosityABSTRACT
The intriguing structure of tagetitoxin (1), a long-standing challenge in natural product synthesis, has been the subject of multiple revisions and has been confirmed through total synthesis. The route commences from a renewable furan starting material and features a number of unusual transformations (such as rearrangements, bromocyclization, and P(V)-based phosphate installation) to arrive at the target in 15 steps. As the route was designed to enable access to both enantiomers, the absolute configuration of the natural product could be assigned using a bioassay on (+)-1 and (-)-1.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Organophosphorus Compounds/chemical synthesis , Dicarboxylic Acids/chemistry , Molecular Structure , Organophosphorus Compounds/chemistry , StereoisomerismABSTRACT
A chemo-enzymatic approach for the conversion of oleic acid into azelaic and pelargonic acid is herein described. It represents a sustainable alternative to ozonolysis, currently employed at the industrial scale to perform the reaction. Azelaic acid is produced in high chemical purity in 44% isolation yield after three steps, avoiding column chromatography purifications. In the first step, the lipase-mediated generation of peroleic acid in the presence of 35% H2O2 is employed for the self-epoxidation of the unsaturated acid to the corresponding oxirane derivative. This intermediate is submitted to in situ acid-catalyzed opening, to afford 9,10-dihydroxystearic acid, which readily crystallizes from the reaction medium. The chemical oxidation of the diol derivative, using atmospheric oxygen as a stoichiometric oxidant with catalytic quantities of Fe(NO3)3â9âH2O, (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO), and NaCl, affords 9,10-dioxostearic acid which is cleaved by the action of 35% H2O2 in mild conditions, without requiring any catalyst, to give pelargonic and azelaic acid.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Fatty Acids/chemical synthesis , Oleic Acid/chemistry , Biocatalysis , Fatty Acids, Unsaturated/chemistry , Lipase/chemistry , Molecular Structure , Oxidation-ReductionABSTRACT
BACKGROUND: The scaffold for head and neck reconstruction needs mechanical strength to maintain specific forms. Hydroxyapatite (HA) enhances the mechanical strength of hydrogel and is routinely used for cartilage regeneration. However, there is a demand for hydroxyapatite that controls chondrogenic cell behavior. OBJECTIVE: Our aim was to regulate HA morphology through a hydrothermal process using organic acid and enhance chondrocyte proliferation and differentiation using shaped-regulated HA. METHODS: HA was synthesized from dodecanedioic acid (DD:HA) and oleic acid (OA:HA) by a hydrothermal method and then coated onto glass plates. Surface properties of the samples were compared by various techniques. Surface roughness and contact angles were calculated. Proliferation and differentiation of chondrogenic cells were measured by MTT assays and Alcian Blue staining, respectively, after various incubation periods. RESULTS: The morphological structures of DD:HA and OA:HA were different; however, the crystallinity and chemical structures were similar. Surface roughness and hydrophilic behavior were higher on DD:HA. DD:HA enhanced chondrogenic cell proliferation over time. The differentiation of ATDC5 cells was also increased on the DD:HA surface compared with those in other groups. CONCLUSIONS: DD:HA enhanced cell viability to a greater extent than OA:HA did, indicating its excellent potential as an inorganic material compatible with chondrocyte regeneration.
Subject(s)
Biocompatible Materials/chemistry , Chondrocytes/cytology , Chondrogenesis , Dicarboxylic Acids/chemistry , Durapatite/chemistry , Animals , Biocompatible Materials/chemical synthesis , Cell Differentiation , Cell Line , Cell Proliferation , Dicarboxylic Acids/chemical synthesis , Durapatite/chemical synthesis , Mice , Surface Properties , Tissue Scaffolds/chemistryABSTRACT
Furancarboxylic acids are promising biobased building blocks in pharmaceutical and polymer industries. In this work, dual-enzyme cascade systems composed of galactose oxidase (GOase) and alcohol dehydrogenases (ADHs) are constructed for controlled synthesis of 5-formyl-2-furancarboxylic acid (FFCA) and 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF), based on the catalytic promiscuity of ADHs. The byproduct H2 O2 , which is produced in GOase-catalyzed oxidation of HMF to 2,5-diformylfuran (DFF), is used for horseradish peroxidase (HRP)-mediated regeneration of the oxidized nicotinamide cofactors for subsequent oxidation of DFF promoted by an ADH, thus implementing H2 O2 internal recycling. The desired products FFCA and FDCA are obtained with yields of more than 95 %.
Subject(s)
Alcohol Dehydrogenase/metabolism , Furaldehyde/analogs & derivatives , Furans/chemical synthesis , Biocatalysis , Dicarboxylic Acids/chemical synthesis , Furaldehyde/chemistry , Furans/chemistry , Galactose Oxidase , Hydrogen Peroxide , Oxidation-ReductionABSTRACT
A one pot-two step procedure for the synthesis of diethyl furan-2,5-dicarboxylate (DEFDC) starting from mucic acid without isolation of the intermediate furan dicarboxylic acid (FDCA) was studied. Then, the production of three different kinds of furan-based polyesters- polyethylene-2,5-furan dicarboxylate (PEF), polyhydropropyl-2,5-furan dicarboxylate(PHPF) and polydiglycerol-2,5-furandicarboxylate (PDGF)-was realized through a Co(Ac)2·4H2O catalyzed polytransesterification performed at 160 °C between DEFDC and a defined diol furan-based prepolymer or pure diglycerol. In parallel to polymerization process, an unattended regioselective 1-OH acylation of glycerol by direct microwave-heated FDCA diester transesterification led to the formation of a symmetric prepolymer ready for further polymerization and clearly identified by 2D NMR sequences. Furthermore, the synthesis of a more soluble and hydrophilic diglycerol-based furanic polyester was also achieved. The resulting biobased polymers were characterized by NMR, FT-IR spectroscopy, DSC, TGA and XRD. The morphologies of the resulted polymers were observed by FE-SEM and the purity of the material by EDX.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Furans/chemical synthesis , Sugar Acids/chemistry , Catalysis , Dicarboxylic Acids/chemistry , Esterification , Furans/chemistry , Magnetic Resonance Spectroscopy , Polymerization , Solvents/chemistryABSTRACT
Aerobic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) as a bioplastics monomer is efficiently promoted by a simple system based on a nonprecious-metal catalyst of MnO2 and NaHCO3. Kinetic studies indicate that the oxidation of 5-formyl-2-furancarboxylic acid (FFCA) to FDCA is the slowest step for the aerobic oxidation of HMF to FDCA over activated MnO2. We demonstrate through combined computational and experimental studies that HMF oxidation to FDCA is largely dependent on the MnO2 crystal structure. Density functional theory (DFT) calculations reveal that vacancy formation energies at the planar oxygen sites in α- and γ-MnO2 are higher than those at the bent oxygen sites. ß- and λ-MnO2 consist of only planar and bent oxygen sites, respectively, with lower vacancy formation energies. Consequently, ß- and λ-MnO2 are likely to be good candidates as oxidation catalysts. On the other hand, experimental studies reveal that the reaction rates per surface area for the slowest step (FFCA oxidation to FDCA) decrease in the order of ß-MnO2 > λ-MnO2 > γ-MnO2 ≈ α-MnO2 > δ-MnO2 > ε-MnO2; the catalytic activity of ß-MnO2 exceeds that of the previously reported activated MnO2 by three times. The order is in good agreement not only with the DFT calculation results, but also with the reduction rates per surface area determined by the H2-temperature-programmed reduction measurements for MnO2 catalysts. The successful synthesis of high-surface-area ß-MnO2 significantly improves the catalytic activity for the aerobic oxidation of HMF to FDCA.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Furaldehyde/analogs & derivatives , Furans/chemical synthesis , Manganese Compounds/chemistry , Oxides/chemistry , Catalysis , Density Functional Theory , Furaldehyde/chemistry , Models, Chemical , Oxidation-Reduction , Oxygen/chemistry , Sodium Bicarbonate/chemistryABSTRACT
Axially chiral binaphthothiophene dicarboxylic acid was prepared as a novel functionalized chiral dicarboxylic acid. The crystal structures of both the racemic form and its salt with chiral diamine revealed the intramolecular S···O interactions (chalcogen bonds) between the sulfur in the naphthothiophene rings and the oxygen of the carboxy groups. The negative-positive and the positive-negative Cotton effects from longer to shorter wavelengths were observed for (R)- and (S)-enantiomers, respectively, in the circular dichroism (CD) spectra.
Subject(s)
Dicarboxylic Acids/chemistry , Thiophenes/chemistry , Dicarboxylic Acids/chemical synthesis , Models, Molecular , Molecular Structure , Stereoisomerism , Thiophenes/chemical synthesisABSTRACT
The quinoline-5,8 dicarboxylic acid scaffold has been identified by a fragment-based approach as new potential lead compound for the development of JMJD3 inhibitors. Among them, 3-(2,4-dimethoxypyrimidin-5-yl)quinoline-5,8-dicarboxylic acid (compound 3) shows low micromolar inhibitory activity against Jumonji domain-containing proteinâ 3 (JMJD3). The experimental evaluation of inhibitory activity against seven related isoforms of JMJD3 highlighted an unprecedented selectivity toward the biological target of interest.
Subject(s)
Dicarboxylic Acids/chemistry , Enzyme Inhibitors/chemistry , Isoenzymes/antagonists & inhibitors , Jumonji Domain-Containing Histone Demethylases/antagonists & inhibitors , Quinolines/chemistry , Binding Sites , Dicarboxylic Acids/chemical synthesis , Enzyme Inhibitors/chemical synthesis , Humans , Isoenzymes/chemistry , Jumonji Domain-Containing Histone Demethylases/chemistry , Molecular Docking Simulation , Molecular Dynamics Simulation , Quinolines/chemical synthesisABSTRACT
2,5-Furandicarboxylic acid (FDCA) is a bio-based platform chemical for the production of polyethylene furanoate (PEF) and other valuable furanic chemicals. A magnetic laccase catalyst with (2,2,6,6-tetramethyl-piperidin-1-yl)oxyl (TEMPO) as the mediator has the remarkable capability of oxidizing 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA). Under optimal reaction conditions, a quantitative yield (90.2 %) of FDCA with complete HMF conversion was obtained after 96â h of reaction. More importantly, the magnetic laccase catalyst exhibited good recyclability and stability, maintaining 84.8 % of its original activity following six reuse cycles. This is the first report on the efficient catalytic oxidation of HMF to FDCA by using an immobilized enzyme catalyst.
Subject(s)
Dicarboxylic Acids/chemical synthesis , Enzymes, Immobilized/chemistry , Furaldehyde/analogs & derivatives , Furans/chemical synthesis , Laccase/chemistry , Magnetite Nanoparticles/chemistry , Biocatalysis , Cyclic N-Oxides/chemistry , Furaldehyde/chemistry , Green Chemistry Technology/methods , Oxidation-Reduction , Silicon Dioxide/chemistryABSTRACT
Following a supramolecular synthon rationale, a dicarboxylic acid derivative having a naphthalenediimide (NDI) core, namely, bis-N-carboxymethyl naphthalenediimide (NDI-G), was reacted with n-alkyl amines with varying alkyl chain lengths to generate a new series of primary ammonium dicarboxylate (PAD) salts. The majority of the salts (≈85 %) were found to gel various polar solvents. The gels were characterized by dynamic rheology and high-resolution electron microscopy. Single-crystal and powder X-ray diffraction analyses were used to study the supramolecular synthon present in one of the gelator salts (i.e., S8). Charge-transfer (CT)-induced gelation with donor molecules such as anthracene methanol (Ant) and pyrene (Py) was also possible with S8. The CT complex (S8.Ant) displayed anticancer activity as probed by cell migration assay on the highly aggresive breast cancer cell line MDA-MB-231. The DMSO gel of S8.Ant also displayed semiconducting behavior. To the best of our knowledge, simple organic salts with an NDI core that display such mulitifunctional properties are hitherto unknown.
Subject(s)
Ammonium Compounds/pharmacology , Antineoplastic Agents/pharmacology , Dicarboxylic Acids/pharmacology , Imides/pharmacology , Naphthalenes/pharmacology , Semiconductors , Amines/chemistry , Amines/pharmacology , Ammonium Compounds/chemical synthesis , Ammonium Compounds/chemistry , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Cell Line, Tumor , Cell Movement/drug effects , Cell Proliferation/drug effects , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/chemistry , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Imides/chemistry , Molecular Structure , Naphthalenes/chemistry , Salts/chemical synthesis , Salts/chemistry , Salts/pharmacology , Structure-Activity RelationshipABSTRACT
Teneraic acid (piperidine-2,6-dicarboxylic acid) is a naturally occurring imino acid that comprises three stereoisomers due to its two asymmetric centers at C2 and C6. The configuration of natural teneraic acid is reported to correspond to trans-(2S,6S). However, a few studies are focused on the stereospecific synthesis of trans-(2S,6S)-teneraic acid. The present study investigates a convenient synthetic method that includes regiospecific anodic oxidation and stereospecific cobalt-catalyzed carbonylation to obtain trans-(2S,6S)-teneraic acid. Methyl (S)-N-benzoyl-α-methoxypipecolate, the key intermediate that displays a structure that corresponds to an intermediate (N-α-hydroxyalkyl amide) of intramolecular amidocarbonylation, was obtained via an anodic oxidation of methyl (S)-N-benzoylpipecolate. Subsequently, cobalt-catalyzed carbonylation converted the methyl (S)-N-benzoyl-α-methoxypipecolate to trans-(2S,6S)-N-benzoyl-teneraic acid dimethyl ester in good optical purity (>95% enantiomeric excess (ee)) and modest yield (63%). Finally, de-protection occurred via acidic hydrolysis to obtain trans-(2S,6S)-teneraic acid. The stereochemistry of synthesized teneraic acid was confirmed as corresponding to trans-(2S,6S) by comparing its physical properties with those of a cis-meso-isomer and those of a trans-(2S,6S)-isomer that were reported in previous studies.
Subject(s)
Cobalt/chemistry , Dicarboxylic Acids/chemistry , Piperidines/chemistry , Aldehydes/chemistry , Amides/chemistry , Catalysis , Circular Dichroism , Dicarboxylic Acids/chemical synthesis , Oxidation-Reduction , StereoisomerismABSTRACT
Herein a facile method is reported to prepare polymer gels based on the formation of acylhydrazone bond under mild conditions. A pillar[5]arene derivative appended with ten hydrazide groups provides multiple sites for the reaction with the aldehyde groups of bis(p-formylphenyl) sebacate in the presence of a small amount of HCl as the catalyst in dimethyl sulfoxide (DMSO), producing transparent polymer organogels. The mechanical properties of gels can be easily tuned by the molar ratio of the reactant compounds. After solvent exchange from DMSO to water, translucent polymer hydrogels with dramatically enhanced strength and stiffness are obtained. The tensile breaking stress and Young's modulus of hydrogels are 20-60 and 1.2-2.7 MPa, respectively, 100 and 20 times those of the corresponding organogels. These robust hydrogels with ultrahigh stiffness should find applications such as in load-bearing artificial organs. This work should merit designing functional materials using other macrocycles.
Subject(s)
Decanoic Acids/chemistry , Dicarboxylic Acids/chemistry , Hydrazines/chemistry , Hydrogels/chemistry , Quaternary Ammonium Compounds/chemistry , Schiff Bases/chemistry , Calixarenes , Catalysis , Decanoic Acids/chemical synthesis , Dicarboxylic Acids/chemical synthesis , Dimethyl Sulfoxide/chemistry , Elastic Modulus , Hydrogels/chemical synthesis , Magnetic Resonance Spectroscopy , Microscopy, Electron, ScanningABSTRACT
A series of 11 unsymmetrical dicarboxylate conjugates of dinucleoside reverse transcriptase inhibitors were synthesized. Three dicarboxylic acids, succinic acid, suberic acid and 1,14-tetradecandioc acid, were diesterified with either 3'-azido-2',3'-dideoxythymidine (AZT), 3'-fluoro-2',3'-dideoxythymidine (FLT), 2',3'-dideoxy-3'-thiacytidine (3TC), or 5-fluoro-2',3'-dideoxy-3'-thiacytidine (FTC). The anti-HIV activity of synthesized compounds was evaluated against HIV-1 X4 (IIIB) and R5 (BaL) viral strains in single-round infection assays. Results indicated that the tetradecandioate esters of nucleosides were more active against HIV than the corresponding parent nucleosides and nucleoside conjugates. The tetradecandioate conjugate of FLT and FTC (5) was found to be the most potent compounds with EC50 values of 47 and 75nM against X4 and R5 HIV-1 strains, respectively, while the EC50 values for the parent analogs, FLT and FTC, ranged from 700 to 3300nM.
Subject(s)
Anti-HIV Agents/chemistry , Anti-HIV Agents/pharmacology , HIV Reverse Transcriptase/antagonists & inhibitors , Nucleosides/chemistry , Nucleosides/pharmacology , Reverse Transcriptase Inhibitors/chemistry , Reverse Transcriptase Inhibitors/pharmacology , Anti-HIV Agents/chemical synthesis , Dicarboxylic Acids/chemical synthesis , Dicarboxylic Acids/chemistry , Dicarboxylic Acids/pharmacology , Esters/chemical synthesis , Esters/chemistry , Esters/pharmacology , HIV Infections/drug therapy , HIV-1/drug effects , Humans , Nucleosides/chemical synthesis , Reverse Transcriptase Inhibitors/chemical synthesisABSTRACT
5-Hydroxymethylfurfural (HMF) is a versatile intermediate in biomass conversion pathways. However, the notoriously unstable nature of HMF imposes challenges to design selective routes to chemicals such as furan-2,5-dicarboxylic acid (FDCA). Here, a new strategy for obtaining furans is presented, bypassing the formation of the unstable HMF. Instead of starting with glucose/fructose and thus forming HMF as an intermediate, the new route starts from uronic acids, which are abundantly present in many agro residues such as sugar beet pulp, potato pulp, and citrus peels. Conversion of uronic acids, via ketoaldonic acids, to the intermediate formylfuroic acid (FFA) esters, and subsequently to FDCA esters, proceeds without formation of levulinic acid or insoluble humins. This new route provides an attractive strategy to valorize agricultural waste streams and a route to furanic building blocks without the co-production of levulinic acid or humins.
Subject(s)
Dicarboxylic Acids/chemistry , Dicarboxylic Acids/chemical synthesis , Esters/chemistry , Furans/chemistry , Furans/chemical synthesis , Levulinic Acids/chemistry , Catalysis , Chemistry Techniques, Synthetic , Isomerism , Oxidation-Reduction , Solvents/chemistryABSTRACT
Poly(anhydride) are unstable and prone to hydrolytic degradation and depolymerisation via anhydride interchange. They are stored at -20°C, packed under inert atmosphere until use. We synthesized a new poly(anhydride) from ricinoleic (RA) and sebacic (SA) acid with alternating ester-anhydride structure that is stable at 25°C for over 18months. The copolymer is also stable in chloroform solution and under γ-irradiation. The polymer hydrolyses through anhydride cleavage lasting ~7days to form oligoesters, which are stable for >30days. The release of gentamycin from the synthesized alternate polymer matrix is sustained compared to the random copolymer.