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1.
J Pharm Biomed Anal ; 53(4): 884-94, 2010 Dec 01.
Article in English | MEDLINE | ID: mdl-20638213

ABSTRACT

A multivariate model was constructed by correlating Raman spectral data with coated amount of the API diprophylline using Partial Least Squares. In agreement with ICH guideline Q2 the method was validated in order to achieve the requirement of demonstrating that Raman spectroscopy is suitable as rapid PAT tool for inline quantitative monitoring of active coating. The present work presents an appropriate approach to transfer the requirements of the guidelines to the Raman method used for inline measurements and demonstrates that the requirements of the validation characteristics were achieved.


Subject(s)
Spectrum Analysis, Raman/methods , Tablets/analysis , Technology, Pharmaceutical , Dyphylline/analysis , Guidelines as Topic , Least-Squares Analysis , Reproducibility of Results , Sensitivity and Specificity , Time Factors
2.
Drug Dev Ind Pharm ; 36(2): 234-43, 2010 Feb.
Article in English | MEDLINE | ID: mdl-19778159

ABSTRACT

BACKGROUND: Active coating is a specific application of film coating where the active ingredient is comprised in the coating layer. This implementation is a challenging operation regarding the achievement of desired amount of coating and coating uniformity. To guarantee the quality of such dosage forms it is desirable to develop a tool that is able to monitor the coating operation and detect the end of the process. METHOD: Coating experiments were performed at which the model drug diprophylline is coated in a pan coater on placebo tablets and tablets containing the active ingredient itself. During the active coating Raman spectra were recorded in-line. The spectral measurements were correlated with the average weight gain and the amount of coated active ingredient at each time point. The developed chemometric model was tested by monitoring further coated batches. Furthermore, the effects of pan rotation speed and working distance on the acquired Raman signal and, hence, resulting effect of the chemometric model were examined. RESULTS: Besides coating on placebo cores it was possible to determine the amount of active ingredient in the film when coated onto cores containing the same active ingredient. In addition, the method is even applicable when varying the process parameters and measurement conditions within a restricted range. CONCLUSION: Raman spectroscopy is an appropriate process analytical technology too.


Subject(s)
Drug Compounding/methods , Tablets, Enteric-Coated/analysis , Technology, Pharmaceutical/methods , Chemistry, Pharmaceutical , Drug Carriers , Dyphylline/analysis , Spectrum Analysis, Raman
3.
AAPS PharmSciTech ; 9(3): 991-7, 2008.
Article in English | MEDLINE | ID: mdl-18758966

ABSTRACT

The stability of hydroxypropyl methylcellulose acetate succinate (HPMC-AS) and its potential incompatibility with active pharmaceutical ingredients (API) carrying hydroxyl group(s) were investigated in this research. HPMC-AS may undergo hydrolysis under harsh processing conditions with the generation of succinic acid and acetic acid, which can form ester bond(s) with the hydroxyl group(s) in API. In this case, the hot-melt extrusion (HME) product prepared from HPMC-AS and our model compound (compound A) was tested after heating at 140 degrees C up to 5 h. The succinate esters of compound A and its epimer were found in the product, suggesting potential drug-excipient incompatibility during formulation development. In addition, dyphylline was also tested with HPMC-AS and the potential incompatibility was further confirmed.


Subject(s)
Drug Incompatibility , Excipients/chemistry , Methylcellulose/analogs & derivatives , Chemistry, Pharmaceutical , Chromatography, Liquid , Dyphylline/analysis , Dyphylline/chemistry , Esterification , Excipients/analysis , Hydrolysis , Methylcellulose/analysis , Methylcellulose/chemistry , Tandem Mass Spectrometry
4.
J AOAC Int ; 91(1): 39-51, 2008.
Article in English | MEDLINE | ID: mdl-18376584

ABSTRACT

Multivariate spectrophotometric calibration and liquid chromatographic (LC) methods were applied to the determination of 2 multicomponent mixtures containing diprophylline, guaiphenesin, methylparaben, and propylparaben (Mixture 1), or clobutinol, orciprenaline, saccharin sodium, and sodium benzoate (Mixture 2). For the multivariate spectrophotometric calibration methods, principal component regression (PCR) and partial least-squares regression (PLS-1), a calibration set of the mixtures consisting of the components of each mixture was prepared in 0.1 M HCl. Analytical figures of merit such as sensitivity, selectivity, limit of quantitation, and limit of detection were determined for both PLS-1 and PCR. The LC separation was achieved on a reversed-phase C18 analytical column by using isocratic elution with 20 mM potassium dihydrogen phosphate, pH 3.3-acetonitrile (55 + 45, v/v) as the mobile phase and UV detection at 260 and 220 nm for Mixture 1 and Mixture 2, respectively. The proposed methods were validated and successfully applied to the analysis of pharmaceutical formulations and laboratory-prepared mixtures containing the 2 multicomponent combinations.


Subject(s)
Antitussive Agents/analysis , Chromatography, Liquid/methods , Spectrophotometry, Ultraviolet/methods , Dyphylline/analysis , Guaifenesin/analysis , Parabens/analysis , Sodium Benzoate/analysis
5.
J Pharm Biomed Anal ; 46(1): 52-7, 2008 Jan 07.
Article in English | MEDLINE | ID: mdl-17936535

ABSTRACT

The following first-order rate constants of the degradation of ertapenem in INVANZ and meropenem in MERONEM were determined: (a) in dry air at 363, 373, 378, 383, 388, 393 K; (b) at increased relative air humidity (76.4% RH) at 313, 323, 333 and 343 K; (c) at increased relative air humidity (50.9, 60.5, 66.5, 76.4% RH-ertapenem and 50.9, 66.5, 76.4 and 90.0% RH-meropenem) at 333 K. The dependence ln k(i) = f(RH%) was described by the equations: ln k(i) = (6.63+/-1.22) x 10(-2) x (RH%)-13.36 +/- 1.68 (ertapenem) and ln k(i) = (4.22 +/- 2.98) x 10(-2) x (RH%)-12.14 +/- 2.16 (meropenem). The dependence lnk(i)=f(1/T) was described by equations: ln k(i) =19.4 +/- 2.6-(9230 +/- 800)(1/T) for ertapenem, at 76.4% RH; ln k(i) = 11.5 +/- 4.9-(9880 +/- 1800)(1/T) for ertapenem in dry air; ln k(i) = 14.8 +/- 11.9-(7785 +/- 3905)(1/T) for meropenem, at 76.4% RH; ln k(i) = 37.6 +/- 7.73-(18385 +/- 2930)(1/T) for meropenem in dry air. The thermodynamic parameters E(a), DeltaH( not equal) and DeltaS( not equal) of the degradation of ertapenem and meropenem were calculated. The difference between the influence of temperature on the stability of ertapenem and meropenem was not significant at 76.4% RH. In dry air (363-393 K) this influence was greater in the case of meropenem. The degradation of ertapenem was slower in this temperature range. Humidity was a significant factor affecting the degradation of these antibiotics and it influenced their stability is similar ways.


Subject(s)
Pharmaceutical Preparations/chemistry , Thienamycins/chemistry , beta-Lactams/chemistry , Algorithms , Chromatography, High Pressure Liquid/methods , Drug Packaging/methods , Drug Stability , Drug Storage/methods , Dyphylline/analysis , Dyphylline/chemistry , Entropy , Ertapenem , Humidity , Meropenem , Molecular Structure , Pharmaceutical Preparations/analysis , Pharmaceutical Preparations/standards , Reference Standards , Tandem Mass Spectrometry/methods , Temperature , Thienamycins/analysis , Time Factors , Validation Studies as Topic , beta-Lactams/analysis
6.
Farmaco ; 60(9): 745-53, 2005 Sep.
Article in English | MEDLINE | ID: mdl-16023119

ABSTRACT

Three methods are developed for the simultaneous determination of diprophylline (DP), phenobarbitone (PH) and papaverine hydrochloride (PP). The chromatographic method depends on a high performance liquid chromatographic (HPLC) separation on a reversed-phase C18 column with a mobile phase consisting of 0.02 M potassium dihydrogen phosphate, pH 3.5--acetonitrile (55:45 v/v). Quantitation was achieved with UV detection at 210 nm based on peak area. The other two chemometric methods applied were principal component regression (PCR) and partial least squares (PLS-1). These approaches were successfully applied to quantify the three drugs in the mixture using the information included in the UV absorption spectra of appropriate solutions in the range 215-245 nm with the intervals Delta lambda = 0.2 nm. The calibration PCR and PLS-1 models were evaluated by internal validation (prediction of compounds in its own designed training set of calibration), by cross-validation (obtaining statistical parameters that show the efficiency for a calibration fit model) and by external validation over laboratory-prepared mixtures and pharmaceutical preparations. The PCR and PLS-1 methods require neither any separation step, nor any priori graphical treatment of the overlapping spectra of the three drugs in a mixture. The results of PCR and PLS-1 methods were compared with HPLC method obtained in pharmaceutical formulation and a good agreement was found.


Subject(s)
Bronchodilator Agents/analysis , Chromatography, High Pressure Liquid/methods , Dyphylline/analysis , Papaverine/analysis , Pharmaceutical Preparations/chemistry , Phenobarbital/analysis , Reference Standards , Reproducibility of Results , Sensitivity and Specificity , Spectrophotometry, Ultraviolet
7.
Article in English | MEDLINE | ID: mdl-14522037

ABSTRACT

A simple micellar electrokinetic chromatography is described for well resolution of theophylline, dyphylline and caffeine. The separation was performed at 25 degrees C using a background electrolyte consisting of 10mM borate buffer at pH 9 and 40 mM sodium dodecyl sulfate (SDS) as running buffer. Under this condition, good separation with high efficiency and short analyses time required is achieved. Several parameters affecting the separation of the drugs were studied, including the pH and concentrations of the borate buffer and sodium dodecyl sulfate. Using caffeine as an internal standard (I.S.), the linear range of the method for the determination of theophylline and dyphylline was over 0.03-1 micromol ml(-1); the detection limit (signal-to-noise ratio 3; injection 0.3 psi, 3s) was 0.01 and 0.02 micromol ml(-1), respectively.


Subject(s)
Chromatography, Micellar Electrokinetic Capillary/methods , Dyphylline/analysis , Theophylline/analysis , Calibration , Hydrogen-Ion Concentration , Reference Standards , Reproducibility of Results
8.
Anal Bioanal Chem ; 374(1): 33-8, 2002 Sep.
Article in English | MEDLINE | ID: mdl-12207237

ABSTRACT

A kinetic-spectrophotometric method for the determination of theophylline, dyphylline and proxyphylline, based on their azo coupling reaction with the diazonium ion of sulfanilic acid after a treatment with alkali, is proposed. The absorbance is recorded from 340 to 600 nm every second during reaction for 90 s, and calibration is performed by partial least-squares regression, using first derivative spectra values. Mixtures containing 2.5-13 micro g mL(-1) dyphylline and proxyphylline, and 2-9 micro g mL(-1) theophylline were successfully resolved with root mean squared errors of prediction (RMSEP) of 0.4, 0.3, and 0.2 for dyphylline, proxyphylline, and theophylline, respectively. The proposed method was satisfactorily applied to the determination of the three compounds in a commercially available pharmaceutical preparation and provided results similar to those obtained by HPLC.


Subject(s)
Aminophylline/analogs & derivatives , Aminophylline/analysis , Dyphylline/analysis , Spectrophotometry, Ultraviolet/methods , Theophylline/analysis , Bronchodilator Agents/analysis , Chromatography, High Pressure Liquid , Hydrogen-Ion Concentration , Kinetics , Least-Squares Analysis , Sensitivity and Specificity
9.
Electrophoresis ; 23(4): 578-83, 2002 Feb.
Article in English | MEDLINE | ID: mdl-11870768

ABSTRACT

Theophylline(1,3-dimethylxanthine), dyphylline [7-(2,3-dihydroxypropyl)theophylline] and proxyphylline [7-(beta-hydroxypropyl)theophylline] are three bronchodilators administered jointly in a single pharmaceutical preparation used against asthma. A micellar electrokinetic chromatography (MEKC) method for their resolution using a background electrolyte consisting of 20 mM tetraborate at pH 8.5 and 100 mM sodium dodecyl sulfate is proposed. The method was used to determine the three active principles in a pharmaceutical preparation. The small amount of sample required and the expeditiousness of the procedure allow content uniformity to be determined in individual tablets. The values of the validation parameters for the method (viz. selectivity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness) are reported. A complete factor design (2(3)x2) including pH, the surfactant concentration and the ionic strength of the background electrolyte as factors was used to estimate robustness. Based on the results, the method is robust enough for quantitation purposes.


Subject(s)
Aminophylline/analogs & derivatives , Bronchodilator Agents/administration & dosage , Chromatography, Micellar Electrokinetic Capillary/methods , Aminophylline/administration & dosage , Aminophylline/analysis , Bronchodilator Agents/analysis , Drug Combinations , Drug Compounding/instrumentation , Drug Compounding/methods , Dyphylline/administration & dosage , Dyphylline/analysis , Linear Models , Methods , Quality Control , Reproducibility of Results , Sensitivity and Specificity , Theophylline/administration & dosage , Theophylline/analysis
10.
Biomed Chromatogr ; 6(3): 141-2, 1992.
Article in English | MEDLINE | ID: mdl-1525489

ABSTRACT

The content and dissolution rate of theophylline, diprophylline and proxyphylline from a sustained release formulation were determined by UV in situ densitometry. After separation the chromatographic zones corresponding to the spots of theophylline, diprophylline and proxyphylline on the high performance thin layer chromatographic plates were scanned in reflectance/absorbance mode at 275 nm. Quantification was performed with a second degree polynomial function over the range 40-200 ng for theophylline and 60-300 ng for diprophylline and proxyphylline. Percentages of dissolved theophylline, diprophylline and proxyphylline were monitored over 1, 3 and 6 h. The method was found to be simple, accurate, reliable, time-saving (up to 18 samples can be determined simultaneously) and low-cost.


Subject(s)
Aminophylline/analogs & derivatives , Dyphylline/analysis , Theophylline/analysis , Aminophylline/administration & dosage , Aminophylline/analysis , Chemical Phenomena , Chemistry, Physical , Chromatography, Thin Layer , Delayed-Action Preparations , Dyphylline/administration & dosage , Indicators and Reagents , Reference Standards , Solubility , Theophylline/administration & dosage
13.
J Pharm Sci ; 74(2): 184-7, 1985 Feb.
Article in English | MEDLINE | ID: mdl-3989689

ABSTRACT

Four diesters and four monoesters of dyphylline were synthesized as prodrugs proposed to prolong the duration of action of dyphylline. They were characterized by IR, 1H NMR, HPLC, and MS. Appropriate solvent-programming conditions for the HPLC separation of dyphylline and the newly synthesized mono- and diesters were developed. It was confirmed by low-temperature 1H NMR at approximately -40 degrees C that all four monoesters were located on the primary hydroxy position. Attempts to produce the secondary monoesters yielded the primary monoesters during purification. Monoesters were shown by HPLC and MS to migrate between the primary and secondary hydroxy groups in aqueous solution.


Subject(s)
Dyphylline/chemical synthesis , Theophylline/analogs & derivatives , Chemistry, Pharmaceutical , Chromatography, High Pressure Liquid , Dyphylline/administration & dosage , Dyphylline/analysis , Magnetic Resonance Spectroscopy , Mass Spectrometry , Spectrophotometry, Infrared
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