ABSTRACT
The marine microorganisms thraustochytrids have been explored for their potential in the production of various bioactive compounds, such as DHA, carotenoids, and squalene. Squalene is a secondary metabolite of the triterpenoid class and is known for its importance in various industrial applications. The bioinformatic analysis for squalene synthase (SQS) gene (the first key enzyme in the tri-terpenoid synthesis pathway), that is prevailing among thraustochytrids, is poorly investigated. In-silico studies combining sequence alignments and bioinformatic tools helped in the preliminary characterization of squalene synthases found in Aurantiochytrium limacinum. The sequence contained highly conserved regions for SQS found among different species indicated the enzyme had all the regions for its functionality. The signal peptide sequence and transmembrane regions were absent, indicating an important aspect of the subcellular localization. Secondary and 3-D models generated using appropriate templates demonstrated the similarities with SQS of the other species. The 3-D model also provided important insights into possible active, binding, phosphorylation, and glycosylation sites.
Subject(s)
Aquatic Organisms/enzymology , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/genetics , Stramenopiles/enzymology , Amino Acid Sequence , Aquatic Organisms/genetics , Binding Sites , Computational Biology , Ligands , Models, Molecular , Molecular Structure , Stramenopiles/geneticsABSTRACT
Camellia vietnamensis Huang, which belongs to Camellia oleifera, is a traditional Chinese medicinal plant widely planted on Hainan Island. Tea saponin is an important functional component of C. vietnamensis, and squalene is the precursor substance that controls its formation. Squalene synthase (SQS: EC 2.5.1.21) synthesizes squalene from 2 molecules of farnesyl pyrophosphate (FPP). In this study, 1683 bp of the C. vietnamensis SQS gene, designated as CvSQS, was cloned and encoded 414 amino acids. Bioinformatics and phylogenetic tree analysis revealed the high homology of CvSQS with squalene synthases from other plants. For soluble proteins, the carboxy-terminal deleted CvSQS was obtained for expression in Escherichia coli Transetta (DE3), and the recombinant protein with a weight of 42.5 kDa was detected using SDS-PAGE and Western blot. After an enzymatic reaction, the presence of squalene in the product was analyzed using GC-MS detection, which indicated that CvSQS had catalytic activity. The tissue specificity of CvSQS and its presence in seeds at various ripening stages was detected by q-RT PCR. CvSQS had the highest transcriptional level in leaves, followed by seeds, roots, and flowers; the amount of CvSQS in the seeds was highest in September. The identification and functional characterization of CvSQS is essential for further studies on the regulation mechanism of tea saponin in C. vietnamensis.
Subject(s)
Camellia , Saponins , Camellia/genetics , Camellia/metabolism , Cloning, Molecular , DNA, Complementary , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Phylogeny , Squalene/metabolism , TeaABSTRACT
Methicillin resistant Staphylococcus aureus is considered multidrug resistant bacterium due to developing biofilm formation associated with antimicrobial resistance mechanisms. Therefore, inhibition of biofilm formation is an alternative therapeutic action to control MRSA infections. The present study revealed the non-antibacterial biofilm inhibitory potential of hesperidin against ATCC strain and clinical isolates of S. aureus. Hesperidin is a flavanone glycoside commonly found in citrus fruit. Hesperidin has been shown to exhibits numerous pharmacological activities. The present study aimed to evaluate the antibiofilm and antivirulence potential of hesperidin against MRSA. Results showed that hesperidin treatment significantly impedes lipase, hemolysin, autolysin, autoaggregation and staphyloxanthin production. Reductions of staphyloxanthin production possibly increase the MRSA susceptibility rate to H2O2 oxidative stress condition. In gene expression study revealed that hesperidin treatment downregulated the biofilm-associated gene (sarA), polysaccharide intracellular adhesion gene (icaA and icaD), autolysin (altA), fibronectin-binding protein (fnbA and fnbB) and staphyloxanthin production (crtM). Molecular docking analysis predicted the ability of hesperidin to interact with SarA and CrtM proteins involved in biofilm formation and staphyloxanthin production in MRSA.
Subject(s)
Bacterial Proteins/metabolism , Biofilms/drug effects , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Hesperidin/pharmacology , Methicillin-Resistant Staphylococcus aureus/drug effects , Methicillin-Resistant Staphylococcus aureus/physiology , Trans-Activators/metabolism , Xanthophylls/metabolism , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/chemistry , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Gene Expression Regulation, Bacterial , Hesperidin/chemistry , Humans , Methicillin-Resistant Staphylococcus aureus/chemistry , Microbial Sensitivity Tests , Molecular Docking Simulation , Staphylococcal Infections/drug therapy , Staphylococcal Infections/microbiology , Trans-Activators/chemistry , Virulence/drug effects , Virulence Factors/metabolismABSTRACT
Lilium 'Siberia' is a perennial herbaceous plant that is commercially significant because of its snowy white floral color and appealing scent which is mainly due to the presence of monoterpenes and benzoids compounds in floral volatile profile. In the current study, LoTPS5 was cloned and functionally characterized. Results revealed that LoTPS5 specifically generates squalene from FPP, whereas no product was produced when it was incubated with GPP or GGPP. The subcellular localization experiment showed that LoTPS5 was located in plastids. Furthermore, LoTPS5 showed its high expression in the leaf followed by petals and sepals of the flower. Moreover, the expression of LoTPS5 gradually increased from the bud stage and peak at the full-bloom stage. Besides, LoTPS5 showed a diurnal circadian rhythmic pattern with a peak in the afternoon (16:00) followed by deep night (24:00) and morning (8:00), respectively. LoTPS5 is highly responsive to mechanical wounding by rapidly elevating its mRNA transcript level. The current study will provide significant information for future studies of terpenoid and squalene biosynthesis in Lilium 'Siberia'.
Subject(s)
Farnesyl-Diphosphate Farnesyltransferase/genetics , Lilium/enzymology , Lilium/genetics , Amino Acid Sequence , Biosynthetic Pathways , Cloning, Molecular , Farnesyl-Diphosphate Farnesyltransferase/analysis , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Gene Expression , Gene Expression Regulation, Plant , Lilium/chemistry , Lilium/metabolism , Odorants/analysis , Phylogeny , Sequence Alignment , Squalene/metabolismABSTRACT
BACKGROUND: Clinopodium gracile (Benth.) Matsum (C. gracile) is an annual herb with pharmacological properties effective in the treatment of various diseases, including hepatic carcinoma. Triterpenoid saponins are crucial bioactive compounds in C. gracile. However, the molecular understanding of the triterpenoid saponin biosynthesis pathway remains unclear. RESULTS: In this study, we performed RNA sequencing (RNA-Seq) analysis of the flowers, leaves, roots, and stems of C. gracile plants using the BGISEQ-500 platform. The assembly of transcripts from all four types of tissues generated 128,856 unigenes, of which 99,020 were mapped to several public databases for functional annotation. Differentially expressed genes (DEGs) were identified via the comparison of gene expression levels between leaves and other tissues (flowers, roots, and stems). Multiple genes encoding pivotal enzymes, such as squalene synthase (SS), or transcription factors (TFs) related to triterpenoid saponin biosynthesis were identified and further analyzed. The expression levels of unigenes encoding important enzymes were verified by quantitative real-time PCR (qRT-PCR). Different chemical constituents of triterpenoid saponins were identified by Ultra-Performance Liquid Chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). CONCLUSIONS: Our results greatly extend the public transcriptome dataset of C. gracile and provide valuable information for the identification of candidate genes involved in the biosynthesis of triterpenoid saponins and other important secondary metabolites.
Subject(s)
Magnoliopsida/genetics , Saponins/biosynthesis , Transcriptome , Triterpenes/metabolism , Biosynthetic Pathways/genetics , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Magnoliopsida/enzymology , Magnoliopsida/metabolism , RNA-Seq , Real-Time Polymerase Chain Reaction , Saponins/chemistry , Secondary Metabolism/genetics , Transcription Factors/genetics , Transcription Factors/metabolism , Triterpenes/chemistryABSTRACT
The quality of alfalfa, a main forage legume worldwide, is of great importance for the dairy industry and is affected by the content of triterpene saponins. These natural terpenoid products of triterpene aglycones are catalyzed by squalene synthase (SQS), a highly conserved enzyme present in eukaryotes. However, there is scare information on alfalfa SQS. Here, an open reading frame (ORF) of SQS was cloned from alfalfa. Sequence analysis showed MsSQS had the same exon/intron composition and shared high homology with its orthologs. Bioinformatic analysis revealed the deduced MsSQS had two transmembrane domains. When transiently expressed, GFP-MsSQS fusion protein was localized on the plasma membrane of onion epidermal cells. Removal of the C-terminal transmembrane domain of MsSQS improved solubility in Escherichia coli. MsSQS was preferably expressed in roots, followed by leaves and stems. MeJA treatment induced MsSQS expression and increased the content of total saponins. Overexpression of MsSQS in alfalfa led to the accumulation of total saponins, suggesting a correlation between MsSQS expression level with saponins content. Therefore, MsSQS is a canonical squalene synthase and contributes to saponin synthesis in alfalfa. This study provides a key candidate gene for genetic manipulation of the synthesis of triterpene saponins, which impact both plant and animal health.
Subject(s)
Farnesyl-Diphosphate Farnesyltransferase/genetics , Genes, Plant , Medicago sativa/enzymology , Medicago sativa/genetics , Acetates/pharmacology , Amino Acid Sequence , Cell Membrane/metabolism , Cloning, Molecular , Cyclopentanes/pharmacology , Escherichia coli/metabolism , Exons/genetics , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Gene Expression Regulation, Plant/drug effects , Green Fluorescent Proteins/metabolism , Introns/genetics , Onions/cytology , Oxylipins/pharmacology , Phylogeny , Plant Epidermis/cytology , Plants, Genetically Modified , Protein Domains , Protein Structure, Secondary , Saponins/metabolism , SolubilityABSTRACT
Tea contains high quantities and diverse types of triterpenoids, particularly in the form of saponins. However, little is yet known about the molecular basis of triterpenoid biosynthesis in tea plant. Here we report on isolation and functional analysis of squalene synthase (SQS) gene from tea plant (Camellia sinensis var. sinensis), which controls the biosynthesis of triterpenoids precursor. First, a full-length cDNA of squalene synthase, designated CsSQS, was isolated from tea plant. The protein is highly homologous to SQSs from other plants. Using CsSQS-reporter assays, CsSQS was demonstrated to be endoplasmic reticulum membrane-bound. The coding region of CsSQS excluding transmemberane sequence was expressed in Escherichia coli. Recombinant CsSQS catalyzed the formation of squalene using farnesyl-pyrophosphate (FPP) as substrate with NADPH and Mg2+. In tea plant leaves, CsSQS expression was significantly induced by both herbivore and mechanical damages. Consistent with the stronger induction of CsSQS expression by mechanical damage than herbivory, tea plants injured mechanically released squalene as a volatile compound, which however was not detected from herbivore-damaged tea plants. Furthermore, it was found that the flowers of another tea plant cultivar Camellia sinensis var. assamica contain higher concentrations of squalene than the cultivar sinensis, indicating variations among tea plant varieties. With the identification and molecular characterization of squalene synthase in tea plant, next, we can ask the questions about the roles of squalene as a volatile product as well as a precursor for triterpenoids, which may promote product development from diverse tea materials and mining of excellent tea germplasm resources.
Subject(s)
Camellia sinensis/enzymology , Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism , Camellia sinensis/genetics , Camellia sinensis/physiology , Cloning, Molecular , Endoplasmic Reticulum/metabolism , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Flowers/metabolism , Gene Expression Regulation, Plant , Herbivory , Phylogeny , Plant Proteins/chemistry , Plants, Genetically Modified , Squalene/analysis , Squalene/metabolism , Stress, Physiological , Nicotiana/genetics , Volatile Organic Compounds/analysis , Volatile Organic Compounds/metabolismABSTRACT
Squalene synthase (SQS) is a potential target for hyperlipidemia treatment. To identify novel chemical scaffolds of SQS inhibitors, we generated 3D-QSAR pharmacophore models using HypoGen. The best quantitative pharmacophore model, Hypo 1, was selected for virtual screening using two chemical databases, Specs and Traditional Chinese Medicine database (TCM). The best-mapped hit compounds were then subjected to filtering by Lipinski's rule of five and docking studies to refine the hits. Finally, five compounds were selected from the top-ranked hit compounds for SQS inhibitory assay in vitro. Three of these compounds could inhibit SQS in vitro, and should be further evaluated pre-clinically as a treatment for hyperlipidemia.
Subject(s)
Enzyme Inhibitors/metabolism , Farnesyl-Diphosphate Farnesyltransferase/antagonists & inhibitors , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Catalytic Domain , Datasets as Topic , Drug Design , Drug Evaluation, Preclinical , Enzyme Inhibitors/chemistry , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Molecular Docking Simulation , Molecular Structure , Protein Binding , Quantitative Structure-Activity RelationshipABSTRACT
Dryopteris fragrans (L.) Schott is a traditional herbal medicine containing medicinal sterols and triterpenoids. Squalene synthase (SQS) is the first crucial enzyme in the biosynthesis pathway of sterols and triterpenoids. The full-length cDNA named DfSQS1 was isolated by RACE. It was predicted that DfSQS1 contained an open reading frame (ORF) of 1239 bp coding 412 amino acid residues with molecular weight of 46.6â¯kDa. It had 18 potential phosphorylation sites, 1 potential N-glycosylation site and 2 transmembrane domains. In neighbor-joining (NJ) phylogenetic tree, DfSQS1 was away from branch of gymnosperms and angiosperms. One hydrophobic domain at the C-terminal of DfSQS1 was deleted to express soluble recombinant enzyme. The truncated DfSQS1 (tDfSQS1) was expressed in Escherichia coli BL21 (DE3). Then, tDfSQS1 was obtained and incubated with farnesyl diphosphate (FPP) to identify its enzymatic activity. The result demontrated that squalene, the product of enzyme catalyzed reaction, was detected by HPLC. Quantitative real-time PCR (qRT-PCR) analysis revealed that the transcription level of DfSQS1 in D. fragrans was the highest in roots, followed by leaves and rhizomes. This work is the first report on cloning, characteration and expression of SQS from D. fragrans. It will be helpful to understand the regulatory role of SQS on the biosynthesis of triterpenoids in the fern.
Subject(s)
Dryopteris/genetics , Farnesyl-Diphosphate Farnesyltransferase/genetics , Plant Proteins/genetics , Amino Acid Sequence , Cloning, Molecular/methods , Dryopteris/chemistry , Dryopteris/metabolism , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Genes, Plant , Models, Molecular , Open Reading Frames , Phylogeny , Plant Proteins/chemistry , Plant Proteins/metabolismABSTRACT
INTRODUCTION: In the present research work, a pharmacophore based virtual screening was performed using Discovery Studio 2.1 for the discovery of some novel molecules as inhibitors of Squalene Synthase Enzyme, a key enzyme in cholesterol biosynthetic pathway. METHODS: A quantitative pharmacophore HypoGen was generated and the best HypoGen had two ring aromatic and one hydrogen bond acceptor lipid features. The best HypoGen showed a very good correlation coefficient (r = 0.901) with satisfactory cost analysis. Furthermore, the HypoGen was validated externally by predicting the activity of test set. The developed model was found to be predictive as it showed low error of prediction for test set molecules. The developed model was used as a search query for virtually screening two chemical databases: sample database from catalyst and minimaybridge. RESULTS AND DISCUSSION: The best hit with good fit value and low predicted activity was further modified to design novel drug-like molecules, which were able to bind to Squalene synthase enzyme active site. CONCLUSION: The best scoring molecule, compound 67 showed 53% inhibition of the human Squalene synthase enzyme, isolated from the cell lysates of Human Hepatoma Cell Line, at a dose of 10 mcg with an IC50 value of 9.43 µm.
Subject(s)
Computer-Aided Design , Drug Design , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Farnesyl-Diphosphate Farnesyltransferase/antagonists & inhibitors , Small Molecule Libraries/chemistry , Small Molecule Libraries/pharmacology , Catalytic Domain/drug effects , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Hep G2 Cells , Humans , Molecular Docking Simulation , Quantitative Structure-Activity RelationshipABSTRACT
Celastrol is an active triterpenoid compound derived from Tripterygium wilfordii which is well-known as a traditional Chinese medicinal plant. Squalene synthase has a vital role in condensing two molecules of farnesyl diphosphate to form squalene, a key precursor of triterpenoid biosynthesis. In the present study, T. wilfordii squalene synthase (TwSQS) was cloned followed by prokaryotic expression and functional verification. The open reading frame cDNA of TwSQS was 1242 bp encoding 413 amino acids. Bioinformatic and phylogenetic analysis showed that TwSQS had high homology with other plant SQSs. To obtain soluble protein, the truncated TwSQS without the last 28 amino acids of the carboxy terminus was inductively expressed in Escherichia coliTransetta (DE3). The purified protein was detected by SDS-PAGE and Western blot analysis. Squalene was detected in the product of in vitro reactions by gas chromatograph-mass spectrometry, which meant that TwSQS did have catalytic activity. Organ-specific and inducible expression levels of TwSQS were detected by quantitative real-time PCR. The results indicated that TwSQS was highly expressed in roots, followed by the stems and leaves, and was significantly up-regulated upon MeJA treatment. The identification of TwSQS is important for further studies of celastrol biosynthesis in T. wilfordii.
Subject(s)
Cloning, Molecular , Farnesyl-Diphosphate Farnesyltransferase , Gene Expression Regulation, Enzymologic/physiology , Gene Expression Regulation, Plant/physiology , Plant Proteins , Tripterygium , Farnesyl-Diphosphate Farnesyltransferase/biosynthesis , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/genetics , Plant Leaves/enzymology , Plant Leaves/genetics , Plant Proteins/biosynthesis , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Roots/enzymology , Plant Roots/genetics , Tripterygium/enzymology , Tripterygium/geneticsABSTRACT
Recently, the biosynthetic pathway for lycopadiene, a C40 tetraterpenoid hydrocarbon, was deciphered from the L race of Botryococcus braunii, an alga that produces hydrocarbon oils capable of being converted into combustible fuels. The lycopadiene pathway is initiated by the squalene synthase (SS)-like enzyme lycopaoctaene synthase (LOS), which catalyzes the head-to-head condensation of two C20 geranylgeranyl diphosphate (GGPP) molecules to produce C40 lycopaoctaene. LOS shows unusual substrate promiscuity for SS or SS-like enzymes by utilizing C15 farnesyl diphosphate (FPP) and C20 phytyl diphosphate in addition to GGPP as substrates. These three substrates can be combined by LOS individually or in combinations to produce six different hydrocarbons of C30, C35, and C40 chain lengths. To understand LOS substrate and product specificity, rational mutagenesis experiments were conducted based on sequence alignment with several SS proteins as well as a structural comparison with the human SS (HSS) crystal structure. Characterization of the LOS mutants in vitro identified Ser276 and Ala288 in the LOS active site as key amino acids responsible for controlling substrate binding, and thus the promiscuity of this enzyme. Mutating these residues to those found in HSS largely converted LOS from lycopaoctaene production to C30 squalene production. Furthermore, these studies were confirmed in vivo by expressing LOS in E. coli cells metabolically engineered to produce high FPP and GGPP levels. These studies also offer insights into tetraterpene hydrocarbon metabolism in B. braunii and provide a foundation for engineering LOS for robust production of specific hydrocarbons of a desired chain length.
Subject(s)
Chlorophyta/enzymology , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Microalgae/enzymology , Polyisoprenyl Phosphates/metabolism , Squalene/metabolism , Terpenes/metabolism , Amino Acid Sequence , Biosynthetic Pathways , Chlorophyta/chemistry , Chlorophyta/metabolism , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Humans , Microalgae/chemistry , Microalgae/metabolism , Models, Molecular , Sequence Alignment , Sesquiterpenes/metabolismABSTRACT
OBJECTIVES: To clone and characterize the squalene synthase from Siraitia grosvenorii (SgSQS). RESULTS: The gene encoding SgSQS was cloned. SgSQS has 417 amino acid residues with an pI of 7.3. There are 32 phosphorylation sites in its sequence: S48 as well as S196 play important roles in regulation of enzyme activity. The enzyme is a monomeric protein with a cave-like active center formed by α helixes and has two transmembrane domains at its C-terminus. SgSQS mRNA expression in stem and root were about twice as much as that in leaf and peel. Full-length SgSQS with measurable catalytic activity was expressed in Escherichia coli. SgSQS activity was optimal at 37 °C and pH 7.5 respectively. CONCLUSION: SgSQS gene was cloned, and the molecular structure and biochemical function of SgSQS were characterized.
Subject(s)
Cucurbitaceae/enzymology , Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Catalytic Domain , Cloning, Molecular , Cucurbitaceae/genetics , Cucurbitaceae/metabolism , Enzyme Stability , Escherichia coli/genetics , Escherichia coli/metabolism , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Gene Expression , Gene Expression Profiling , Hydrogen-Ion Concentration , Isoelectric Point , Phosphorylation , Plant Leaves/enzymology , Plant Roots/enzymology , Plant Stems/enzymology , Protein Conformation , Protein Processing, Post-Translational , RNA, Messenger/analysis , TemperatureABSTRACT
As the first step of ongoing efforts to investigate the genes responsible for the biosynthesis of steroidal saponins in the medicinal plant Ornithogalum caudatum, this investigation reported the cDNA isolation, prokaryotic expression and functional characterization of squalene synthase (SQS) gene from O. caudatum for the first time. Specifically, two unigenes showing high sequence identity to SQS were retrieved from RNA-Taq data, and then a full-length OcSQS1 corresponding to the two unigenes was isolated from O. caudatum genome by a nested PCR assay. The open reading frame of OcSQS1 was 1230 bp and encoded a polypeptide of 409 aa. OcSQS1 was predicted to be a membrane-bound protein with at least four conserved motifs associated with binding, regulatory and catalytic activities of OcSQS1 and two transmembrane domains. Next, many attempts to generate soluble OcSQS1 in heterologous Escherichia coli were made, including optimization of expression conditions, application of varied expression plasmids with different tags, secretory peptides and molecular chaperones, and truncated mutation of OcSQS1. Finally, the successful availability of a soluble, truncated OcSQS1 mutant was achieved by combinational use of the utensils from the vast genetic toolbook. Moreover, this truncated OcSQS1 mutant retained the folding capability as well as its catalytic activity, converting FPP to form squalene. Importantly, the present research tentatively verified the involvement of the second transmembrane domain in the proper folding of the recombinant OcSQS1 protein.
Subject(s)
Cloning, Molecular , DNA, Complementary , Escherichia coli/metabolism , Farnesyl-Diphosphate Farnesyltransferase , Ornithogalum/genetics , Plant Proteins , DNA, Complementary/genetics , DNA, Complementary/isolation & purification , Escherichia coli/genetics , Farnesyl-Diphosphate Farnesyltransferase/biosynthesis , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/isolation & purification , Ornithogalum/enzymology , Plant Proteins/biosynthesis , Plant Proteins/chemistry , Plant Proteins/genetics , Plant Proteins/isolation & purification , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purificationABSTRACT
Panax notoginseng (Burk.) F. H. Chen, which is a used traditional Chinese medicine known as Sanqi or Tianqi in China, is widely studied for its ability to accumulate the triterpene saponins. Squalene synthase (SS: EC 2.5.1.21) catalyzes the first enzymatic step from the central isoprenoid pathway toward sterol and triterpenoid biosynthesis. In this study, SS from P. notoginseng was cloned and investigated followed by its recombinant expression and preliminary enzyme activity. The nucleotide sequence of the ORF contains 1 248 nucleotides and encodes 415 amino acid residues with molecular weight of 47.16kDa and pI of 6.50. Bioinformatics analysis revealed that the deduced PnSS protein had a high similarity with other plant squalene synthases. To obtain soluble recombinant enzymes, 29 hydrophobic amino acids were deleted from the carboxy terminus and expressed as GST-Tag fusion protein in Escherichia coli BL21 (DE3). Approximately 66.46kDa recombinant protein was checked on SDS-PAGE and Western Blot analysis. Preliminary activity of the resultant bacterial crude extract was analyzed by gas chromatograph-mass spectrometer (GC-MS). The identification and function of PnSS is important for further studies of the triterpene saponins biosynthesis in P. notoginseng.
Subject(s)
Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Panax notoginseng/enzymology , Panax notoginseng/genetics , Amino Acid Sequence , Base Sequence , Cloning, Molecular , Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/isolation & purification , Humans , Models, Molecular , Phylogeny , Protein Conformation , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolismABSTRACT
Many drugs for the treatment of hypercholesterolemia are targeting the enzymes involved in human cholesterol biosynthesis pathway. Squalene synthase, the rate-limiting enzyme located at the downstream of cholesterol synthesis pathway, has become a better candidate to develop next-generation hypocholesterolemia drugs. In the present study, we cloned and expressed the recombinant human squalene synthase (hSQS) as the lure to isolate potential peptide inhibitors from screening the conformation-constrained phage-displayed cyclic peptide c7c library. Their binding capabilities were further estimated by ELISA. Their pharmaceutical potentials were then analyzed through molecular modeling and the ADMET property evaluations. Four ennea-peptides and nine tetra-peptides were finally synthesized to evaluate their inhibitory potentials toward hSQS. The results indicate that the ennea-peptide CLSPHSMFC, tetra-peptides SMFC, CKTE, and WHQW can effectively inhibit hSQS activities (IC50 values equal to 64, 76, 87, and 90 µM, respectively). These peptides may have potentials to develop future cholesterol-lowering therapeutics. The ligand-protein interaction analysis also reveals that the inner hydrophobic pocket could be a more critical site of hSQS.
Subject(s)
Enzyme Inhibitors/isolation & purification , Farnesyl-Diphosphate Farnesyltransferase/antagonists & inhibitors , Peptide Library , Peptides, Cyclic/isolation & purification , Bacteriophages/chemistry , Bacteriophages/genetics , Cholesterol/metabolism , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Enzyme-Linked Immunosorbent Assay/methods , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Humans , Hypercholesterolemia/drug therapy , Ligands , Peptides, Cyclic/chemistry , Peptides, Cyclic/pharmacologyABSTRACT
Terpenoids, a class of isoprenoids usually isolated from plants, are always used as commercial flavor and anticancer drugs. As a key precursor for triterpenes and sterols, biosynthesis of squalene (SQ) can be catalyzed by squalene synthase (SQS) from two farnesyl diphosphate molecules. In this work, the key SQS gene involved in sterols synthesis by Mortierella alpine, an industrial strain often used to produce unsaturated fatty acid such as γ-linolenic acid and arachidonic acid, was identified and characterized. Bioinformatic analysis indicated that MaSQS contained 416 amino acid residues involved in four highly conserved regions. Phylogenetic analysis revealed the closest relationship of MaSQS with Ganoderma lucidum and Aspergillus, which also belonged to the member of the fungus. Subsequently, the recombinant protein was expressed in Escherichia coli BL21(DE3) and detected by SDS-PAGE. To improve the expression and solubility of protein, 17 or 27 amino acids in the C-terminal were deleted. In vitro activity investigation based on gas chromatography-mass spectrometry revealed that both the truncated enzymes could functionally catalyze the reaction from FPP to SQ and the enzymatic activity was optimal at 37 °C, pH 7.2. Moreover, based on the site-directed mutagenesis, the mutant enzyme mMaSQSΔC17 (E186K) displayed a 3.4-fold improvement in catalytic efficiency (k(cat)/K(m)) compared to the control. It was the first report of characterization and modification of SQS from M. alpine, which facilitated the investigation of isoprenoid biosynthesis in the fungus. The engineered mMaSQSΔC17 (E186K) can be a potential candidate of the terpenes and steroids synthesis employed for synthetic biology.
Subject(s)
Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Mortierella/enzymology , Mutagenesis, Site-Directed , Amino Acid Sequence , Aspergillus/enzymology , Conserved Sequence , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Farnesyl-Diphosphate Farnesyltransferase/analysis , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Gas Chromatography-Mass Spectrometry , Mortierella/genetics , Phylogeny , Recombinant Proteins/analysis , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Reishi/enzymology , Sequence Deletion , SolubilityABSTRACT
According to the designed specific primers of gene fragment based on the Salvia miltiorrhiza transcriptome data, a full-length cDNA sequence of SQS2 from S. miltiorrhiza f. alba was cloned by the method of reverse transcription polymerase chain reaction (RT-PCR). The SmSQS2 cDNA sequence was obtained, this sequence is named SmSQS2 and its GenBank registration number is KM244731. The full length of SmSQS2 cDNA was 1245 bp, encoding 414 amino acids including 5'UTR 115 bp and 3'UTR 237 bp. Sequence alignment and phylogenetic analysis demonstrated that SmSQS2 had relative close relationship to the SQS2 of S. miltiorrhiza. The induction of E. coli [pET28-SQS2] in different temperature, induction time, IPTG concentrations and density of inducing host bacterium (A600) were performed, Shaking the culture at 30 degrees C until the A600 is approximately 0.6 and add IPTG to final concentration of 0.2 mmol x L(-1), and then the optimal expression of SmSQS2 recombinant protein were accumulated after the induction time of 20 h. The research provided important base for the study of sterol and terpene biosynthesis of SQS2 in S. miltiorrhiza f. alba.
Subject(s)
Cloning, Molecular , Farnesyl-Diphosphate Farnesyltransferase/genetics , Plant Proteins/genetics , Salvia miltiorrhiza/enzymology , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Models, Molecular , Molecular Sequence Data , Phylogeny , Plant Proteins/chemistry , Plant Proteins/metabolism , Salvia miltiorrhiza/chemistry , Salvia miltiorrhiza/classification , Salvia miltiorrhiza/genetics , Sequence AlignmentABSTRACT
The triterpene squalene is a key metabolic intermediate for sterols, hopanoids and various other triterpenoids. The biosynthesis of squalene is catalyzed by squalene synthase (SQS), which converts two molecules of farnesyl diphosphate to squalene. In this study, a lycophyte Selaginella moellendorffii was found to emit squalene as a volatile compound under a number of conditions that mimic biotic stresses. Searching the genome sequence of S. moellendorffii led to the identification of a putative squalene synthase gene. It was designated as SmSQS. SmSQS is homologous to known squalene synthases from other plants and animals at both the amino acid level and structural level. Recombinant SmSQS expressed in Escherichia coli catalyzed the formation of squalene using farnesyl diphosphate as substrate. The expression of SmSQS was significantly induced by the same set of stress factors that induced the emission of volatile squalene from S. moellendorffii plants. Taken together, these results support that SmSQS is responsible for the biosynthesis of volatile squalene and volatile squalene may have a role in the defense of S. moellendorffii plants against biotic stresses.
Subject(s)
Selaginellaceae/metabolism , Squalene/metabolism , Volatile Organic Compounds/metabolism , Amino Acid Sequence , Biocatalysis , Escherichia coli/genetics , Farnesyl-Diphosphate Farnesyltransferase/chemistry , Farnesyl-Diphosphate Farnesyltransferase/genetics , Farnesyl-Diphosphate Farnesyltransferase/metabolism , Gene Expression Regulation, Plant , Genes, Plant , Models, Molecular , Molecular Sequence Data , Selaginellaceae/genetics , Sequence Homology, Amino AcidABSTRACT
Squalene is a precursor of thousands of bioactive triterpenoids and also has industrial value as a lubricant, health-promoting agent, and/or drop-in biofuel. To establish an efficient Escherichia coli-based system for squalene production, we tested two different squalene synthases and their mutants in combination with precursor pathways. By co-expressing a chimeric mevalonate pathway with human or Thermosynechococcus squalene synthase, E. coli accumulated squalene up to 230 mg/L or 55 mg/g-DCW in flask culture. We also determined that a significant truncation of squalene synthase at the C-terminus retains partial cellular activity. The squalene-producing strain described herein represents a convenient platform for gene discovery and the construction of the pathway toward natural and non-natural hopanoids/steroids.