ABSTRACT
Both UV and blue light have been reported to regulate the biosynthesis of flavonoids in tea plants; however, the respective contributions of the corresponding regions of sunlight are unclear. Additionally, different tea cultivars may respond differently to altered light conditions. We investigated the responses of different cultivars ('Longjing 43', 'Zhongming 192', 'Wanghai 1', 'Jingning 1' and 'Zhonghuang 2') to the shade treatments (black and colored nets) regarding the biosynthesis of flavonoids. For all cultivars, flavonol glycosides showed higher sensitivity to light conditions compared with catechins. The levels of total flavonol glycosides in the young shoots of different tea cultivars decreased with the shade percentages of polyethylene nets increasing from 70% to 95%. Myricetin glycosides and quercetin glycosides were more sensitive to light conditions than kaempferol glycosides. The principal component analysis (PCA) result indicated that shade treatment greatly impacted the profiles of flavonoids in different tea samples based on the cultivar characteristics. UV is the crucial region of sunlight enhancing flavonol glycoside biosynthesis in tea shoots, which is also slight impacted by light quality according to the results of the weighted correlation network analysis (WGCNA). This study clarified the contributions of different wavelength regions of sunlight in a field experiment, providing a potential direction for slightly bitter and astringent tea cultivar breeding and instructive guidance for practical field production of premium teas based on light regimes.
Subject(s)
Camellia sinensis/growth & development , Flavonoids/biosynthesis , Glycosides/biosynthesis , Plant Shoots/growth & development , Camellia sinensis/radiation effects , Flavonoids/chemistry , Flavonoids/radiation effects , Glycosides/radiation effects , Kaempferols/chemistry , Plant Shoots/radiation effects , Principal Component Analysis , Sunlight , Ultraviolet RaysABSTRACT
Addition of selenium or application of ultraviolet A (UVA) radiation for crop production could be an effective way of producing phytochemical-rich food. This study was conducted to investigate the effects of selenium and UVA radiation, as well as their combination on growth and phytochemical contents in broccoli microgreens. There were three treatments: Se (100 µmol/L Na2SeO3), UVA (40 µmol/m2/s) and Se + UVA (with application of Se and UVA). The control (CK) was Se spraying-free and UVA radiation-free. Although treatment with Se or/and UVA inhibited plant growth of broccoli microgreens, results showed that phytochemical contents increased. Broccoli microgreens under the Se treatment had higher contents of total soluble sugars, total phenolic compounds, total flavonoids, ascorbic acid, Fe, and organic Se and had lower Zn content. The UVA treatment increased the contents of total chlorophylls, total soluble proteins, total phenolic compounds, and FRAP. However, the Se + UVA treatment displayed the most remarkable effect on the contents of total anthocyanins, glucoraphanin, total aliphatic glucosinolates, and total glucosinolates; here, significant interactions between Se and UVA were observed. This study provides valuable insights into the combinational selenium and UVA for improving the phytochemicals of microgreens grown in an artificial lighting plant factory.
Subject(s)
Brassica/growth & development , Crop Production , Phytochemicals/biosynthesis , Selenium/pharmacology , Ascorbic Acid/metabolism , Brassica/drug effects , Brassica/radiation effects , Flavonoids/metabolism , Flavonoids/radiation effects , Iron/metabolism , Phenol/metabolism , Phenol/radiation effects , Phytochemicals/radiation effects , Sugars/metabolism , Sugars/radiation effects , Ultraviolet RaysABSTRACT
Although our previous study revealed that gamma-irradiated chrysin enhanced anti-inflammatory activity compared to intact chrysin, it remains unclear whether the chrysin derivative, CM1, produced by gamma irradiation, negatively regulates toll-like receptor (TLR) signaling. In this study, we investigated the molecular basis for the downregulation of TLR4 signal transduction by CM1 in macrophages. We initially determined the appropriate concentration of CM1 and found no cellular toxicity below 2 µg/mL. Upon stimulation with lipopolysaccharide (LPS), CM1 modulated LPS-stimulated inflammatory action by suppressing the release of proinflammatory mediators (cytokines TNF-α and IL-6) and nitric oxide (NO) and downregulated the mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) signaling pathways. Furthermore, CM1 markedly elevated the expression of the TLR negative regulator toll-interacting protein (Tollip) in dose- and time-dependent manners. LPS-induced expression of cell surface molecules (CD80, CD86, and MHC class I/II), proinflammatory cytokines (TNF-α and IL-6), COX-2, and iNOS-mediated NO were inhibited by CM1; these effects were prevented by the knockdown of Tollip expression. Additionally, CM1 did not affect the downregulation of LPS-induced expression of MAPKs and NF-κB signaling in Tollip-downregulated cells. These findings provide insight into effective therapeutic intervention of inflammatory disease by increasing the understanding of the negative regulation of TLR signaling induced by CM1.
Subject(s)
Flavonoids/pharmacology , Intracellular Signaling Peptides and Proteins/metabolism , Macrophages/metabolism , Animals , Anti-Inflammatory Agents/pharmacology , Flavonoids/metabolism , Flavonoids/radiation effects , Inflammation/drug therapy , Interleukin-6 , Lipopolysaccharides/adverse effects , Lipopolysaccharides/pharmacology , Macrophages/drug effects , Mice , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Nitric Oxide , RAW 264.7 Cells , Signal Transduction/drug effects , Toll-Like Receptor 4/drug effects , Toll-Like Receptor 4/metabolism , Toll-Like Receptors/metabolism , Tumor Necrosis Factor-alphaABSTRACT
Recently, there have been efforts to use ultraviolet-B radiation (UV-B) as a biotechnological tool in greenhouses. Leafy Brassica species are mainly considered for their ability to synthesize glucosinolates and are valued as baby salads. They also have a remarkable concentration of chemically diverse flavonoid glycosides. In this study, the effect of short-term UV-B radiation at the end of the production cycle was investigated without affecting plant growth. The aim was to verify which exposure and adaptation time was suitable and needs to be further investigated to use UV as a biotechnological tool in greenhouse production of Brassica species. It is possible to modify the flavonoid glycoside profile of leafy Brassica species by increasing compounds that appear to have potentially high antioxidant activity. Exemplarily, the present experiment shows that kaempferol glycosides may be preferred over quercetin glycosides in response to UV-B in Brassica rapa ssp. chinensis, for example, whereas other species appear to prefer quercetin glycosides over kaempferol glycosides, such as Brassica oleracea var. sabellica or Brassica carinata. However, the response to short-term UV-B treatment is species-specific and conclusions on exposure and adaptation time cannot be unified but must be drawn separately for each species.
Subject(s)
Adaptation, Physiological , Brassica/metabolism , Flavonoids/metabolism , Glycosides/metabolism , Plant Leaves/metabolism , Ultraviolet Rays/adverse effects , Brassica/classification , Brassica/growth & development , Brassica/radiation effects , Flavonoids/analysis , Flavonoids/radiation effects , Glycosides/analysis , Glycosides/radiation effects , Plant Leaves/growth & development , Plant Leaves/radiation effects , Species Specificity , Time FactorsABSTRACT
BACKGROUND: Black cumin seed contains considerable amounts of bioactive phenolics that have physiological activity and antioxidant potency, which may be boosted by microwave radiation. The core objective of the study was to evaluate the influence of microwave radiation on the antioxidant activity of cumin seed as a function of pretreatment time and extract concentration. METHODS: In this study, total flavonoid content (TFC) and antioxidant activity of methanol (CME), 70% methanol in water (CMW), ethanol (CEE) and 70% ethanol in water (CEW) extracts of microwaved radiated cumin seed were measured. The antioxidant activity was determined using a Folin-Ciocalteu assay, phosphomolybdenum assay, DPPH radical scavenging activity, H2O2 scavenging activity, hydroxyl radical scavenging activity and reducing power. To predict antioxidant potency, FTIR spectra of extracts were also recorded. RESULTS: Microwave pretreatment significantly reduced the TFC with the pretreatment time. Antioxidant activity increased with increasing pretreatment time and extract concentration in all extracts. Aqueous methanolic extract from radiated seeds appeared to display the highest effectiveness. The results from FT-IR of ex- tracts indicating the existence of multiple functional groups were comparable to those obtained from multiple antioxidative assays. The antioxidant effectiveness of the samples was ranked: CMW > CEW > CME > CEE. CONCLUSIONS: Both treatment duration and extract concentration were found to be critical factors in determin- ing the overall quality of the product. The present study revealed important information for using black cumin seed in developing food products with high antioxidant potency.
Subject(s)
Antioxidants/chemistry , Microwaves , Nigella sativa/chemistry , Seeds/chemistry , Antioxidants/radiation effects , Flavonoids/chemistry , Flavonoids/radiation effects , Nigella sativa/radiation effects , Seeds/radiation effects , Time FactorsABSTRACT
Irradiation technology can improve the biological activities of natural molecules through a structural modification. This study was conducted to investigate the enhancement of the anticancer effects of chrysin upon exposure to gamma irradiation. Gamma irradiation induces the production of new radiolytic peaks simultaneously with the decrease of the chrysin peak, which increases the cytotoxicity in HT-29 human colon cancer cells. An isolated chrysin derivative (CM1) exhibited a stronger apoptotic effect in HT-29 cells than intact chrysin. The apoptotic characteristics induced by CM1 in HT-29 cells was mediated through the intrinsic signaling pathway, including the excessive production of included reactive oxygen species, the dissipation of the mitochondrial membrane potential, regulation of the B cell lymphoma-2 family, activation of caspase-9, 3, and cleavage of poly (adenosine diphosphate-ribose) polymerase. Our findings suggest that CM1 can be a potential anticancer candidate for the treatment of colon cancer.
Subject(s)
Antineoplastic Agents/pharmacology , Colonic Neoplasms/metabolism , Flavonoids/pharmacology , Mitochondria/drug effects , Antineoplastic Agents/radiation effects , Caspases/genetics , Caspases/metabolism , Colonic Neoplasms/drug therapy , Colonic Neoplasms/genetics , Flavonoids/radiation effects , Gamma Rays , HT29 Cells , Humans , Membrane Potential, Mitochondrial/drug effects , Mitochondria/metabolism , Poly(ADP-ribose) Polymerases/genetics , Poly(ADP-ribose) Polymerases/metabolism , Reactive Oxygen Species/metabolismABSTRACT
To investigate the effect of post-harvest light irradiation on the accumulation of flavonoids and limonoids, harvested Newhall navel oranges were continuously exposed to light-emitting diode (LED) and ultraviolet (UV) light irradiation for 6 days, and the composition and content of flavonoids and limonoids in the segments were determined using UPLC-qTOF-MS at 0, 6, and 15 days after harvest. In total, six polymethoxylated flavonoids (PMFs), five flavone-O/C-glycosides, seven flavanone-O-glycosides, and three limonoids were identified in the segments. The accumulation of these components was altered by light irradiation. Red and blue light resulted in higher levels of PMFs during exposure periods. The accumulation of PMFs was also significantly induced after white light, UVB and UVC irradiation were removed. Red and UVC irradiation induced the accumulation of flavone and flavanone glycosides throughout the entire experimental period. Single light induced limonoid accumulation during exposure periods, but limonoid levels decreased significantly when irradiation was removed. Principal component analysis showed a clear correlation between PMFs and white light, between flavonoid glycosides and red light and UVC, and between limonoids and UVC. These results suggest that the accumulation of flavonoids and limonoids in citrus is regulated by light irradiation. White light, red light and UVC irradiation might be a good potential method for improving the nutrition and flavor quality of post-harvest citrus.
Subject(s)
Citrus sinensis/metabolism , Flavonoids/radiation effects , Flavoring Agents/radiation effects , Limonins/radiation effects , Chromatography, High Pressure Liquid/methods , Flavanones/metabolism , Flavones/metabolism , Flavonoids/metabolism , Flavoring Agents/metabolism , Glycosides/metabolism , Light , Limonins/metabolism , Principal Component Analysis/methods , Tandem Mass Spectrometry/methods , Time Factors , Ultraviolet RaysABSTRACT
The impact of short-term UV-B treatment on the content of individual flavonoids and photosynthetic pigments in cotyledons and the growth of common buckwheat (Fagopyrum esculentum Moench) seedlings was investigated. Seeds of four common buckwheat cultivars were germinated in darkness over a period of 4 days and acclimatized for 2 days under a 16/8 h light/dark photoperiod at 24/18 °C day/night, and exposure to 100-120 µmol â m-2 â s-1 of photosynthetically active radiation (PAR). Seedlings were divided into three batches, including two batches subjected to different doses of UV-B (5 W â m-2 and 10 W â m-2, one hour per day) for 5 days, and a control group exposed to PAR only. Exposure to UV-B increased anthocyanin levels in the cotyledons of all examined cultivars, it inhibited hypocotyl elongation, but did not affect the content of photosynthetic pigments. Flavone concentrations increased in cv. Red Corolla and Kora, remained constant in cv. Panda and decreased in cv. Hruszowska. Exposure to UV-B decreased rutin levels in cv. Hruszowska, but not in the remaining cultivars. Cultivars Hruszowska, Panda and Kora appeared to be less resistant to UV-B than Red Corolla. Higher resistance to UV-B radiation in Red Corolla can probably be attributed to its higher content of anthocyanins and rutin in comparison with the remaining cultivars.
Subject(s)
Anthocyanins/radiation effects , Carotenoids/radiation effects , Cotyledon/radiation effects , Fagopyrum/radiation effects , Flavones/radiation effects , Flavonols/radiation effects , Seedlings/radiation effects , Ultraviolet Rays , Anthocyanins/metabolism , Carotenoids/metabolism , Chlorophyll/metabolism , Chlorophyll/radiation effects , Cotyledon/metabolism , Fagopyrum/growth & development , Fagopyrum/metabolism , Flavones/metabolism , Flavonoids/metabolism , Flavonoids/radiation effects , Flavonols/metabolism , Hypocotyl/growth & development , Hypocotyl/radiation effects , Phenols/metabolism , Phenols/radiation effects , Rutin/metabolism , Rutin/radiation effects , Seedlings/growth & developmentABSTRACT
Epimedium pseudowushanense B.L.Guo is used in traditional medicine as an aphrodisiac and to strengthen muscles and bones. Several recent reports have shown that flavonoids from Epimedium also significantly affect the treatment of breast cancer, liver cancer, and leukemia. However, few studies have examined the medicinal-ingredient yield of Epimedium, a light-demanding shade herb, under different light intensities. To investigate the effects of light intensity on medicinal-ingredient yields, Epimedium was exposed to five levels of light intensity until harvest time. Leaf dry biomass under L4 was the highest among different light treatments. L4 was also associated with the highest net photosynthetic rate. Quantification of epimedin A, epimedin B, epimedin C, and icariin showed that L3 produced the highest amount of epimedin C, and that flavonoid content responded to light levels differently. Results indicated that L3 and L4 were the optimal light levels for medicinal-ingredient yield.
Subject(s)
Drugs, Chinese Herbal/analysis , Epimedium/growth & development , Flavonoids/analysis , Photosynthesis/radiation effects , Biomass , Epimedium/chemistry , Epimedium/radiation effects , Flavonoids/radiation effects , Light , Plant Leaves/chemistry , Plant Leaves/growth & development , Plant Leaves/radiation effectsABSTRACT
The accumulation of ultraviolet (UV)-absorbing compounds (flavonoids and related phenylpropanoids) and the resultant decrease in epidermal UV transmittance (TUV ) are primary protective mechanisms employed by plants against potentially damaging solar UV radiation and are critical components of the overall acclimation response of plants to changing solar UV environments. Whether plants can adjust this UV sunscreen protection in response to rapid changes in UV, as occurs on a diurnal basis, is largely unexplored. Here, we use a combination of approaches to demonstrate that plants can modulate their UV-screening properties within minutes to hours, and these changes are driven, in part, by UV radiation. For the cultivated species Abelmoschus esculentus, large (30-50%) and reversible changes in TUV occurred on a diurnal basis, and these adjustments were associated with changes in the concentrations of whole-leaf UV-absorbing compounds and several quercetin glycosides. Similar results were found for two other species (Vicia faba and Solanum lycopersicum), but no such changes were detected in Zea mays. These findings reveal a much more dynamic UV-protection mechanism than previously recognized, raise important questions concerning the costs and benefits of UV-protection strategies in plants and have practical implications for employing UV to enhance crop vigor and quality in controlled environments.
Subject(s)
Abelmoschus/radiation effects , Flavonoids/radiation effects , Hibiscus/radiation effects , Solanum lycopersicum/radiation effects , Vicia faba/radiation effects , Zea mays/radiation effects , Abelmoschus/physiology , Acclimatization , Circadian Rhythm , Flavonoids/physiology , Hibiscus/physiology , Solanum lycopersicum/physiology , Plant Epidermis/physiology , Plant Epidermis/radiation effects , Plant Leaves/physiology , Plant Leaves/radiation effects , Sunlight , Ultraviolet Rays , Vicia faba/physiology , Zea mays/physiologyABSTRACT
BACKGROUND: Light is one of the most significant environmental factors affecting to the accumulation of flavonoids in fruits. The composition of the light spectrum has been shown to affect the production of phenolic compounds during fruit ripening. However, specific information on the biosynthesis of flavonoids in fruits in response to different wavelengths of light is still scarce. In the present study bilberry (Vaccinium myrtillus L.) fruits, which are known to be rich with anthocyanin compounds, were illuminated with blue, red, far-red or white light during the berry ripening process. Following the illumination, the composition of anthocyanins and other phenolic compounds was analysed at the mature ripening stage of fruits. RESULTS: All the three monochromatic light treatments had significant positive effect on the accumulation of total anthocyanins in ripe fruits compared to treatment with white light or plants kept in darkness. The elevated levels of anthocyanins were mainly due to a significant increase in the accumulation of delphinidin glycosides. A total of 33 anthocyanin compounds were detected in ripe bilberry fruits, of which six are novel in bilberry (cyanidin acetyl-3-O-galactose, malvidin acetyl-3-O-galactose, malvidin coumaroyl-3-O-galactose, malvidin coumaroyl-3-O-glucose, delphinidin coumaroyl-3-O-galactose, delphinidin coumaroyl-3-O-glucose). CONCLUSIONS: Our results indicate that the spectral composition of light during berry development has significant effect on the flavonoid composition of ripe bilberry fruits.
Subject(s)
Anthocyanins/metabolism , Flavonoids/biosynthesis , Fruit/radiation effects , Light , Phenols/metabolism , Vaccinium myrtillus/radiation effects , Anthocyanins/radiation effects , Chromatography, High Pressure Liquid , Flavonoids/genetics , Flavonoids/radiation effects , Fruit/growth & development , Fruit/metabolism , Phenols/radiation effects , Polymerase Chain Reaction , Tandem Mass Spectrometry , Vaccinium myrtillus/growth & development , Vaccinium myrtillus/metabolismABSTRACT
We discuss on the relative significance of different functional roles potentially served by flavonoids in photoprotection, with special emphasis to their ability to scavenge reactive oxygen species (ROS) and control the development of individual organs and whole plant. We propose a model in which chloroplast-located flavonoids scavenge H2O2 and singlet oxygen generated under excess light-stress, thus avoiding programmed cell death. We also draw a picture in which vacuolar flavonoids in conjunction with peroxidases and ascorbic acid constitute a secondary antioxidant system aimed at detoxifying H2O2, which may diffuse out of the chloroplast at considerable rates and enter the vacuole following excess light stress-induced depletion of ascorbate peroxidase. We hypothesize for flavonols key roles as developmental regulators in early and current-day land-plants, based on their ability to modulate auxin movement and auxin catabolism. We show that antioxidant flavonoids display the greatest capacity to regulate key steps of cell growth and differentiation in eukaryotes. These regulatory functions of flavonoids, which are shared by plants and animals, are fully accomplished in the nM concentration range, as likely occurred in early land plants. We therefore conclude that functions of flavonoids as antioxidants and/or developmental regulators flavonoids are of great value in photoprotection. We also suggest that UV-B screening was just one of the multiple functions served by flavonoids when early land-plants faced an abrupt increase in sunlight irradiance.
Subject(s)
Flavonoids/metabolism , Flavonoids/radiation effects , Plants/metabolism , Plants/radiation effects , Antioxidants/metabolism , Ultraviolet RaysABSTRACT
The photostability of quercetin in alcoholic solutions was studied. Both UVA and UVB light induced degradation of quercetin, yielding a single product 1 deriving from oxidation and addition of an alcohol molecule to the 2,3 double bond. The same mechanism operated when quercetin was dissolved in alkaline solutions, and again a product 2 due to oxidation and addition of water was characterized. Comparison with quercetin analogs confirmed that, despite the presence of five hydroxy groups in quercetin, those in positions 3, 3', and 4' are mainly involved in the antioxidant activity of the compound , as well as in its photolability.
Subject(s)
Quercetin/analogs & derivatives , Quercetin/chemistry , Ultraviolet Rays , Ammonia/chemistry , Apigenin/chemistry , Apigenin/radiation effects , Drug Stability , Ethanol/chemistry , Flavonoids/chemistry , Flavonoids/radiation effects , Hydrogen-Ion Concentration , Kaempferols/chemistry , Kaempferols/radiation effects , Methanol/chemistry , Oxidation-Reduction , Photochemical Processes , Quercetin/radiation effects , Rutin/chemistry , Rutin/radiation effects , Solutions , Solvents/chemistry , Spectrophotometry, UltravioletABSTRACT
Flavonols such as kaempferol and quercetin are believed to provide protection against ultraviolet (UV)-induced damage to plants. Recent in vitro studies have examined the ability of flavonols to protect against UV-induced damage to mammalian cells. Stability of flavonols in cell culture media, however, has been problematic, especially for quercetin, one of the most widely studied flavonols. As part of our investigations into the potential for flavonols to protect skin against UV-induced damage, we have determined the stability of a series of flavonols that differ only in the number of substituents on the B-ring. We measured the stability of these flavonols over time to UVA radiation, Dulbecco's modified Eagle's medium (DMEM), and Dulbecco's phosphate-buffered saline (DPBS) using high performance liquid chromatography with UV detection (HPLC-UV). The identification of the breakdown products of flavonols was accomplished by using a hybrid quadrupole linear ion trap mass spectrometer coupled with liquid chromatography. Tandem mass spectrometric analysis (MS/MS) of flavonol photoproducts was confirmed by comparing with the known standard samples. We have determined that flavonol stability decreases with increasing B-ring substitution, suggesting that future investigation of potential photoprotective flavonols will need to be cognizant of this trend.
Subject(s)
Flavonoids/chemistry , Flavonoids/radiation effects , Ultraviolet Rays , Chromatography, High Pressure Liquid , Drug Stability , Solutions , Spectrometry, Mass, Electrospray Ionization , WaterABSTRACT
Peanut skin, which is removed in the peanut blanching process, is rich in bioactive compounds with antioxidant properties. The aims of this study were to measure bioactive compounds in peanut skins and evaluate the effect of gamma radiation on their antioxidant activity. Peanut skin samples were treated with 0.0, 5.0, 7.5, or 10.0 kGy gamma rays. Total phenolics, condensed tannins, total flavonoids, and antioxidant activity were evaluated. Extracts obtained from the peanut skins were added to refined-bleached-deodorized (RBD) soybean oil. The oxidative stability of the oil samples was determined using the Oil Stability Index method and compared to a control and synthetic antioxidants (100 mg/kg BHT and 200 mg/kg TBHQ). Gamma radiation changed total phenolic content, total condensed tannins, total flavonoid content, and the antioxidant activity. All extracts, gamma irradiated or not, presented increasing induction period (h), measured by the Oil Stability Index method, when compared with the control. Antioxidant activity of the peanut skins was higher than BHT. The present study confirmed that gamma radiation did not affect the peanut skin extracts' antioxidative properties when added to soybean oil.
Subject(s)
Antioxidants/analysis , Antioxidants/radiation effects , Arachis/chemistry , Arachis/radiation effects , Flavonoids/analysis , Flavonoids/radiation effects , Food Irradiation , Gamma Rays , Plant Extracts/chemistry , Plant Extracts/radiation effects , Polyphenols/analysis , Polyphenols/radiation effects , Proanthocyanidins/analysis , Proanthocyanidins/radiation effects , Seeds/chemistry , Seeds/radiation effects , Soybean Oil/chemistryABSTRACT
CONTEXT: Irradiation is the process of exposing food such as herbal plant to ionizing radiation to destroy microorganisms. Zataria multiflora Boiss (Lamiaceae), known as Avishan-e-Shirazi in Persian, is a thyme-like plant that grows naturally in central and southern parts of Iran and is used in traditional folk medicine. OBJECTIVE: In this study, the effects of γ-radiation on chemical composition and antioxidant properties of Z. multiflora were investigated. MATERIALS AND METHODS: The plants were first irradiated with Co60 source (0, 10, and 25 kGy) and then subjected to Clevenger extraction to obtain essential oils. The composition of the oil was analyzed by a gas chromatography and compared with samples pretreated under different conditions. In parallel, the hydroalcoholic extract was prepared and used for measuring flavonoid content. Thereafter, the free-radical scavenging and antioxidant properties of essential oils and hydroalcoholic extract were examined. RESULTS: Despite the minor change in the individual oil constituents, the total percentage of the main components remained unaffected before and after irradiation (~95%). In addition, the total flavonoid content of hydroalcoholic extract was also unchanged due to irradiation (~32 mg QE/g extract). The high radical scavenging activity of the oil (~67%) and hydroalcoholic extract (~71%), in addition, the antioxidant properties of the oil (~91%) and hydroalcoholic extract (~95%), were unaffected after irradiation. DISCUSSION AND CONCLUSIONS: These findings may suggest the sustainability of Z. multiflora extract properties pretreated with γ-radiation. With a view to its antioxidant applications, resistance of Z. multiflora and its properties against radiation effects are promising findings.
Subject(s)
Antioxidants/pharmacology , Gamma Rays , Lamiaceae/chemistry , Plant Extracts/pharmacology , Antioxidants/isolation & purification , Antioxidants/radiation effects , Chromatography, Gas , Dose-Response Relationship, Radiation , Flavonoids/isolation & purification , Flavonoids/radiation effects , Free Radical Scavengers/isolation & purification , Free Radical Scavengers/pharmacology , Free Radical Scavengers/radiation effects , Iran , Lamiaceae/radiation effects , Medicine, Traditional , Oils, Volatile/isolation & purification , Oils, Volatile/pharmacology , Oils, Volatile/radiation effects , Plant Extracts/isolation & purification , Plant Extracts/radiation effectsABSTRACT
Hawthorn seed (HS), an important by-product of the Hawthorn industry, is rich in potentially health-promoting flavonoids compounds. In this paper, the ultrasound-assisted extraction (UAE) of FC from HS was investigated. Important variables and their levels were obtained using Plackett-Burman (PB) design and Box-Behnken (BB) design. A mathematical model was developed to show the effects of each variable and their combinatorial interactions on extraction yield of FC. A high coefficient of determination (R(2) = 91.26%) indicated good agreement between the experimental and predicted values of FC yield. The optimum levels of these significant parameters were determined using response surface methodology (RSM), which revealed these as follows: ultrasound temperature 65 °C, ultrasonic time 37 min, extraction temperature 91 °C, extraction time 1.5h, solid-liquid ratio of 1:18, and 72% ethanol. Under the optimum condition, the UAE rate of FC was up to 91.7%, and the yield of FC was 16.45 ± 0.02 mg/g (P<0.05) that was 1.32-fold the yield of conventional reflux extraction (CRE).
Subject(s)
Crataegus/chemistry , Flavonoids/isolation & purification , Flavonoids/radiation effects , Plant Extracts/isolation & purification , Seeds/chemistry , Seeds/radiation effects , Sonication/methods , Crataegus/radiation effects , Flavonoids/chemistry , High-Energy Shock Waves , Plant Extracts/chemistry , Plant Extracts/radiation effects , Radiation Dosage , Solid Phase Extraction/methodsABSTRACT
The biological activities of garlic may be affected by different processing methods. This study, therefore, aimed to evaluate potential anticancer effects of different type of processed garlic extracts on WEHI-164 tumor cells in inbred BALB/c mice and correlate the tumor growth rates with some garlic constituents. In a preclinical trial 60 BALB/c mice were injected with WEHI-164 tumor cells and divided into six groups of 10 animals. Group 1 mice received 200 µL of saline, and groups 2-6 were injected intraperitoneally with fresh, microwaved, 3-month-old, leaves, and boiled garlic extracts, respectively, at 20 mg/kg/0.2 mL. Three weeks following tumor inoculation, the mean tumor size in garlic extract-treated groups was reduced with significant reductions observed in the fresh and microwaved extract groups compared with the control group (P<.05). The antioxidant capacity and the amounts of allicin, flavonoids, and phenolic compounds in differentially processed garlic were evaluated and correlated with their anticancer activities. There was a linear correlation between the amounts of allicin, flavonoids, or phenolic components derived from fresh, microwaved, 3-month-old, leaves, and boiled garlic and cancer growth prevention. In conclusion, garlic has anticancer activity against WEHI-164 tumor cells, and processing such as heating reduces its effect dramatically. The anticancer activities of different kinds of garlic are related to the level of allicin, flavonoids, and phenolic components. Therefore, fresh garlic has the highest content of bioactive components and the greatest anticancer efficacy.
Subject(s)
Antineoplastic Agents, Phytogenic/therapeutic use , Antioxidants/therapeutic use , Fibrosarcoma/drug therapy , Garlic/chemistry , Plant Extracts/therapeutic use , Plant Roots/chemistry , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/radiation effects , Antioxidants/analysis , Antioxidants/chemistry , Antioxidants/radiation effects , Cell Line, Tumor , Disulfides , Drug Stability , Female , Fibrosarcoma/pathology , Flavonoids/analysis , Flavonoids/radiation effects , Flavonoids/therapeutic use , Hot Temperature/adverse effects , Iran , Mice , Mice, Inbred BALB C , Microwaves/adverse effects , Neoplasm Transplantation , Phenols/analysis , Phenols/radiation effects , Phenols/therapeutic use , Plant Extracts/chemistry , Plant Extracts/radiation effects , Plant Leaves/chemistry , Sulfinic Acids/analysis , Sulfinic Acids/radiation effects , Sulfinic Acids/therapeutic use , Tumor Burden/drug effectsABSTRACT
Fisetin, a bioflavonoid, has important biological relevance. It exhibits intramolecular excited state proton transfer (ESIPT), analogous to the structurally similar flavonoids. The presence of multiple prototropic forms of fisetin was observed at various concentrations of different bile salt molecules. The presence of ground state fisetin anion (FA)(GS) (λ(ex) 418 nm; λ(em) 490 nm) in alcohols and bile salt micellar media is a novel observation. The interaction of fisetin with sodium cholate (NaC) and some other bile salts has been studied in detail, using the intrinsic fluorescence of different prototropic forms of fisetin: neutral form (FN, λ(ex) 369 nm, λ(em) ~ 400 nm), ground state anion form ((FA)(GS), λ(ex) 418 nm, λ(em) 490 nm) and phototautomer (FT, λ(ex) 369 nm, λ(em) 540 nm). The hypsochromic shift of (FA*)(ES) emission and bathochromic shift of FT emission with increasing bile salt concentration suggests the progressive reduction of polarity of the bile salt media, which could be resulting from the neutralization of bile salt molecules as their concentration increases.
Subject(s)
Bile Acids and Salts/chemistry , Flavonoids/chemistry , Sodium Cholate/chemistry , Flavonoids/radiation effects , Flavonols , Micelles , Protons , Spectrometry, Fluorescence , TemperatureABSTRACT
OBJECTIVE: To investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis. METHOD: The content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR. RESULT: Active ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995). CONCLUSION: Active ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.