ABSTRACT
4(5)-methylimidazole (4-MeI) is a potential carcinogen widely used in food colours. EU regulations specify a maximum allowable concentration of 200 ppm for 4-MeI in caramel colours. This study reports an electrochemical determination technique for 4-MeI in caramel colours for the first time. The effect of pH and interference from air were studied to optimize the detection conditions on a glassy carbon electrode in aqueous alkaline solutions using square wave voltammetry (SWV) technique. The concentration of 4-MeI was quantitatively measured down to 10 µM (â¼0.8 ppm). Traditional methods such as HPLC, GC, spectrometry and immunoassays involve either expensive instrumentation and reagents or time consuming preparation and detection processes. This study demonstrates the possibility of rapid and simple electrochemical determination of (4-MeI) in food colours with minimum workup using a portable potentiostat.
Subject(s)
Electrochemical Techniques , Imidazoles , Imidazoles/chemistry , Imidazoles/analysis , Electrochemical Techniques/instrumentation , Food Coloring Agents/analysis , Food Coloring Agents/chemistry , Food Contamination/analysis , Hydrogen-Ion Concentration , CarbohydratesABSTRACT
Quinoline yellow (QY), as a food coloring agent, will consume a large number of detoxifying substances in the body after being ingested by the human body, interfering with the normal metabolic functions of the human body, and may cause allergies, diarrhea and other symptoms, as well as a certain degree of carcinogenicity, posing a great threat to human health. As a result, it is critical to develop a fast, sensitive, and effective approach to determining quinoline yellow in food. In this study, carbon dots (N-CQDs) with high fluorescence quantum yield were prepared and used to determine the QY content using the dual mode of internal filtering effect and fluorescence emission shift detection. Both methods showed good linearity in the range of QY concentration of 0.3-3.2 µM, and the detection limits were classified as 2.6 nM and 0.18 µM. In addition, in order to achieve visual detection of QY, fluorescent test strips were constructed using the carbon dots and non-fluorescent qualitative filter paper to make the detection of QY more convenient. This probe presents a novel way for detecting quinoline yellow in food analysis.
Subject(s)
Carbon , Nitrogen , Quantum Dots , Quinolines , Spectrometry, Fluorescence , Quantum Dots/chemistry , Carbon/chemistry , Spectrometry, Fluorescence/methods , Quinolines/chemistry , Nitrogen/chemistry , Food Coloring Agents/analysis , Limit of Detection , Fluorescent Dyes/chemistryABSTRACT
Some synthetic dyes are fraudulently added into spices to appeal visually to consumers. Food regulations in several countries, including the United States, Australia, Japan and the European Union, strictly prohibit the use of unauthorised synthetic dyes in food. Nevertheless, illegal practices persist, where spices contaminated with potentially carcinogenic dyes have been documented, posing potential health risks to consumers. In the present study, 14 synthetic dyes were investigated through liquid chromatography/tandem mass spectrometry in 252 commercially available spices in the Singapore market. In 18 out of these (7.1%) at least 1 illegal dye was detected at concentrations ranging from 0.010 to 114 mg/kg. Besides potential health risks, presence of these adulterants also reflects the economic motivations behind their fraudulent use. Findings in the present study further emphasise the need for increased public awareness, stricter enforcement, and continuous monitoring of illegal synthetic dyes in spices to ensure Singapore's food safety.
Subject(s)
Food Contamination , Spices , Tandem Mass Spectrometry , Spices/analysis , Singapore , Food Contamination/analysis , Humans , Coloring Agents/analysis , Coloring Agents/chemistry , Food Coloring Agents/analysisABSTRACT
BACKGROUND: Ponceau 4R (E124) and carmoisine (CMS; E122) are frequently utilized azo synthetic dyes in the food industry owing to their aesthetically pleasing coloration and broad consumer acceptability. It is imperative to prioritize environmentally favorable technologies for quantifying these dyes, as excessive consumption of these poses significant health risks. OBJECTIVE: The primary objective of this research was to establish a reversed-phase (RP)-HPLC method that could simultaneously detect Ponceau 4R and CMS, implementing green analytical chemistry (GAC) and analytical quality by design (AQbD), using an ultrasound-assisted extraction (UAE) technique in commercial food samples. METHODS: An Agilent Eclipse Plus column (C18, 250 × 4.6 mm id, 5 µm) was utilized for effective separation with a mobile phase of ethanol-acetate buffer pH 5 (60:40, v/v), flow rate of 1 mL/min, and detection wavelength of 515 nm. Critical variables selected for method optimization were ethanol percentage and flow rate, determined using central composite design (CCD). In order to adhere to the 12 principles of green chemistry, hazardous solvents were substituted with ethanol, which is distinguished by its ease of use, effectiveness, and ecological sustainability. The greenness assessment was conducted utilizing the green analytical procedure index (GAPI), analytical eco-scale (AES), and analytical greenness metrics (AGREE). RESULTS: The respective retention times for Ponceau 4R and CMS were 2.276 and 3.450 min. The recovery rate of Ponceau 4R and CMS fluctuated between 70% and 102% and 80% and 102%, respectively, across various marketed food samples. The procedure passed validation in accordance with the International Conference on Harmonization Q14 guidelines. CONCLUSION: The devised method demonstrates that the validation parameters like linearity, precision, sensitivity, and reproducibility are within the specified limits of ICH guidelines. The greenness assesment tools GAPI, AES, and AGREE produced the most favorable results. HIGHLIGHTS: In future, environmentally sustainable, solvent-based, robust AQbD methodologies for assessing varieties of food colorants may be adopted and improved commercially.
Subject(s)
Azo Compounds , Food Coloring Agents , Green Chemistry Technology , Naphthalenesulfonates , Chromatography, High Pressure Liquid/methods , Green Chemistry Technology/methods , Azo Compounds/analysis , Food Coloring Agents/analysis , Food Analysis/methods , Chromatography, Reverse-Phase/methods , Ultrasonic Waves , UltrasonicsABSTRACT
Aluminum is added to many food colors to change their solubility. This study compares the aluminum-containing food color carmine with its aluminum-free version carminic acid (both E 120), hypothesizing that the addition of aluminum does not only change the color's solubility, but also its effects on human cells. We could show that carmine, but not carminic acid, is taken up by gastrointestinal Caco-2 and umbilical vein endothelial cells (HUVEC). Clear differences between gene expression profiles of Caco-2 cells exposed to carmine, carminic acid or control were shown. KEGG analysis revealed that carmine-specific genes suppress oxidative phosphorylation, and showed that this suppression is associated with neurodegenerative diseases such as Alzheimer and Parkinson disease. Furthermore, carmine, but not carminic acid, increased proliferation of Caco-2 cells. Our findings show that a food color containing aluminum induces different cellular effects compared to its aluminum-free form, which is currently not considered in EU legislation.
Subject(s)
Carmine , Food Coloring Agents , Humans , Carmine/analysis , Aluminum/toxicity , Caco-2 Cells , Endothelial Cells , Food Coloring Agents/analysis , ExcipientsABSTRACT
In recent years, artificial additives, especially synthetic food colorants, were found to demonstrate wider properties compared to their natural equivalents; however, their health impact is still not totally mapped. Our study aimed to determine the long-term (30 and 90 days) exposure effect of one of the commonly used artificial food colorants, tartrazine, on NMRI mice. The applied dose of tartrazine referred to the human equivalent dose for acceptable daily intake (ADI). Further, we evaluated its impact on the transcription of a range of epigenetic effectors, members of the DNA methyltransferase (DNMT) as well as histone deacetylase (HDAC) families. Following the exposure, organ biopsies were collected from the lungs, kidneys, liver, and spleen, and the gene expression levels were determined by real-time quantitative reverse transcription PCR (RT-qPCR). Our results demonstrated significant upregulation of genes in the tested organs in various patterns followed by the intake of tartrazine on ADI. Since DNMT and HDAC genes are involved in different steps of carcinogenesis, have roles in the development of neurological disorders and the effect of dose of everyday exposure is rarely studied, further investigation is warranted to study these possible associations.
Subject(s)
Food Coloring Agents , Neoplasms , Nervous System Diseases , Humans , Mice , Animals , Tartrazine/analysis , Food Coloring Agents/adverse effects , Food Coloring Agents/analysis , Histone Deacetylases/genetics , Histone Deacetylases/metabolism , Mice, Inbred Strains , Neoplasms/geneticsABSTRACT
Safety concerns in the food industry have increased the demand for natural food colorants. However, the application ranges of natural blue colorants are insufficient because they are scarce in nature, and the currently available natural blue dyes are limited to water-soluble products. In this study, we investigated a fat-soluble azulene derivative isolated from the mushroom Lactarius indigo as a potential candidate for a natural blue colorant. We developed its first total synthesis, where the azulene skeleton was constructed from a pyridine derivative and an ethynyl group was converted into an isopropenyl group using zirconium complexes. Moreover, nanoparticles of the azulene derivative were prepared via reprecipitation method, and their colorant ability was investigated in aqueous solutions. The new candidate food colorant exhibited a deep-blue color in an organic solvent and aqueous dispersion.
Subject(s)
Azulenes , Food Coloring Agents , Food Coloring Agents/analysis , Coloring Agents , WaterABSTRACT
This article presents an original planar carbon fiber electrode (PCFE), in which shungite (SHU) is used as a modifier for the first time. Shungite is a unique natural nanostructured composite consisting of carbon in the form of aggregated graphene stacks, oxides of silicon, titanium, aluminum, iron, magnesium, potassium, etc. Macro- and micro-elements, biologically active components that are present in shungite provide it with attractive antioxidant properties, make it a biocompatible and environmentally friendly material that meets the principles of green chemistry. A unique supramolecular structure of shungite carbon presents a multilayer globular-cluster formation with mesopores in the internal volume. It determines specific physical, chemical, catalytic, and adsorption properties of shungite. Carbon fiber with an irregular 3D structure was used as an effective electrode platform for strong immobilization of shungite. The PCFE was fabricated using a simple and scalable hot lamination technology that produces very low cost flexible planar electrodes. The sensor (SHU/PCFE) was characterized by scanning electron microscopy; electrochemical impedance analysis; cyclic, differential-pulse and stripping voltammetry. The SHU/PCFE showed a 2.5-fold increase in the electroactive surface area, a 1.8-fold decrease in the electron transfer resistance compared with the bare PCFE. Under optimal experimental conditions and preconcentration at +0.2 V (vs. Ag/AgCl) 180 s, the developed sensor allowed the quantification of Allura Red in the ranges of 0.001-0.1 and 0.1-2 µmol L-1 with an extremely low detection limit of 0.36 nmol L-1. Moreover, this convenient and cost-effective sensor also has good repeatability, stability and anti-interference ability. The interfering effect of sweeteners and preservatives in the determination of Allura Red does not exceed 3.6%. The practical application of the SHU/PCFE was demonstrated using drink samples, lollipops and pharmaceuticals.
Subject(s)
Food Coloring Agents , Carbon Fiber , Food Coloring Agents/analysis , Azo Compounds/analysis , Carbon/chemistry , Electrodes , Electrochemical Techniques , Limit of DetectionABSTRACT
Surface-enhanced Raman scattering (SERS) with the advantages of high sensitivity, nondestructive analysis, and a unique fingerprint effect shows great potential in point-of-care testing (POCT). However, SERS faces challenges in rapidly constructing a substrate with high repeatability, homogeneity, and sensitivity, which are the key factors that restrict its practical applications. In this study, we propose a one-step chemical printing strategy for synthesizing a three-dimensional (3D) plasmon-coupled silver nanocoral (AgNC) substrate (only need about 5 min) without any pretreatments and complex instruments. The galvanic replacement between AgNO3 and Cu sheets will provide both Ag0 for the formation of silver nanostructures and Cu2+ for the polymerization of fish sperm DNA (FSDNA). The protection of AgNCs is facilitated by the crosslinked FSDNA, which can improve the stability of the substrate and promote the control of its coral-like morphology. The obtained substrate displays excellent capacity of signal enhancement due to the 3D plasmon coupling both between nanocoral tentacles and between nanocorals and Cu sheets as well. Therefore, the AgNC substrates display high activity (enhancement factor = 1.96 × 108) and uniformity (RSD < 6%). Food colorants have been widely used in various foods to improve their color, but the inevitable toxicity of colorants seriously threatens food safety. Therefore, the proposed AgNC substrates were used to directly quantify three kinds of weak-affinity food colorant molecules including Brilliant Blue, Allura Red, and Sunset Yellow assisted by the capture by cysteamine hydrochloride (CA), showing the detection limits (S/N = 3) of 0.053, 0.087, and 0.089 ppm, respectively. The SERS method has been further applied in the detection of the three kinds of food colorants in both complex food samples and urine with recoveries of 91-119%. The satisfactory detection results suggest that the facile preparation strategy of AgNC substrates will be widely used in SERS-based POCT to promote the development of food safety and on-site healthcare.
Subject(s)
Food Coloring Agents , Metal Nanoparticles , Nanostructures , Male , Animals , Silver/chemistry , Food Coloring Agents/analysis , Semen/chemistry , Spectrum Analysis, Raman/methods , Printing, Three-Dimensional , Metal Nanoparticles/chemistryABSTRACT
This study developed a rapid and easy analytical method for the simultaneous determination of nine synthetic colourants (SCs) in capsule dietary supplements. Sample pretreatment involved thermal treatment to dissolve gelatin, using the enzymes protease and amylase to prevent the gelation of gelatin and fat-soluble substances removal using petroleum ether. The method was linear (r2 ≥0.999), with LOD of 0.009-0.029 µg/mL and LOQ of 0.42-1.40 µg/g. Recovery ranged from 90.9 to 108.9%. The relative expanded measurement uncertainty ranged from 4.1 to 6.3%. Allura Red AC (R40) and Brilliant Blue FCF (B1) were commonly detected in 20 of the 28 samples. Up to six SCs such as Tartrazine (Y4), Sunset yellow (Y5), Amaranth (R2), Erythrosine B (R3), R40 and B1 were detected in a single sample, ranging from 30.5 to 40.2 µg/g. Total content of SCs in various capsule supplements ranged from 0.3 to 73.7 µg/g.
Subject(s)
Food Coloring Agents , Food Coloring Agents/analysis , Gelatin , Food Contamination , Dietary Supplements , Republic of KoreaABSTRACT
Colorant tartrazine is widely used in the food industry, but its long-term and excessive consumption is harmful to human health. Therefore, it is necessary to establish a sensitive detection method for tartrazine. Blue fluorescent carbon dots with L-arginine and o-phenylenediamine as precursors, namely L-Arg/oPD-CDs, were prepared via the hydrothermal method. Then, L-Arg/oPD-CDs were further purified by dialysis, thin layer chromatography and column chromatography. A dual-mode nanosensor based on fluorescent and UV absorption was successfully developed. Excellent linear ranges of 0-5 µM and 10-50 µM were obtained with a low detection limit of 42.3 nM based on fluorescence. A good linear range of 0-50 µM was obtained with a low detection limit of 130.15 nM based on UV absorption. The quenching mechanism of tartrazine towards L-Arg/oPD-CDs fluorescence was the inner filter effect. In addition, a dual-mode nanosensor was used for tartrazine determination in millet, maize flour, carbonated drink, and sugar samples. This study provides new insight into the detection of tartrazine by applying a dual-mode nanosensor.
Subject(s)
Food Coloring Agents , Quantum Dots , Humans , Tartrazine/analysis , Carbon/chemistry , Food Coloring Agents/analysis , Quantum Dots/chemistry , Renal DialysisABSTRACT
It is necessary to determine whether synthetic dyes are present in food since their excessive use has detrimental effects on human health. For the simultaneous assessment of tartrazine and Patent Blue V, a novel electrochemical sensing platform was developed. As a result, two artificial azo colorants (Tartrazine and Patent Blue V) with toxic azo groups (-NN-) and other carcinogenic aromatic ring structures were examined. With a low limit of detection of 0.06 µM, a broad linear concentration range 0.09µM to 950µM, and a respectable recovery, scanning electron microscopy (SEM) was able to reveal the excellent sensing performance of the suggested electrode for patent blue V. The electrochemical performance of an electrode can be characterized using cyclic and differential pulse voltammetry, and electrochemical impedance spectroscopy. Moreover, the classification model was created by applying binary classification assessment using enhanced artificial intelligence comprises of support vector machine (SVM) and Genetic Algorithm (GA), respectively, a support vector machine and a genetic algorithm, which was then validated using the 50 dyes test set. The best binary logistic regression model has an accuracy of 83.2% and 81.1%, respectively, while the best SVM model has an accuracy of 90.3% for the training group of samples and 81.1% for the test group (RMSE = 0.644, R2 = 0.873, C = 205.41, and = 5.992). According to the findings, Cu-BTC MOF (copper (II)-benzene-1,3,5-tricarboxylate) has a crystal structure and is tightly packed with hierarchically porous nanomaterials, with each particle's edge measuring between 20 and 37 nm. The suggested electrochemical sensor's analytical performance is suitable for foods like jellies, condiments, soft drinks and candies.
Subject(s)
Artificial Intelligence , Azo Compounds , Electrochemical Techniques , Food Coloring Agents , Food Contamination , Rosaniline Dyes , Tartrazine , Humans , Azo Compounds/analysis , Azo Compounds/isolation & purification , Electrochemical Techniques/methods , Electrodes , Food Coloring Agents/analysis , Food Coloring Agents/isolation & purification , Food Contamination/prevention & control , Rosaniline Dyes/analysis , Rosaniline Dyes/isolation & purificationABSTRACT
The existing strategies for the determination of synthetic food colorants (FCs) in manufactured foods are highly relied on specialized instruments and skilled personnel which are limited by the high technical threshold and instrumentation cost. Herein, highly branched pipette tips (PTs) were fabricated as a robust all-in-one device for high-performance extraction and visual detection of FCs via handy aspiration and dispensing procedures of pipette controller. The density of extraction groups and inner specific surface area of PTs greatly increased after facile physical coating and subsequent layer-by-layer branching reactions, and the maximum increment in binding capacity of PTs was exceeded 300 times at 8-10 iterations of branching layers, enabling the PTs to be colored just by short-time extraction of FCs and to achieve the instrument-independent visual detection of FCs by virtue of their outstanding PT-SPE performance. As a proof-of-concept, the in-situ PT-based solid phase extraction (PT-SPE) with high recoveries (from 91.73 ± 4.76% to 99.90 ± 4.14%) and semiquantitative naked-eye detection of FCs (Allura red and brilliant blue) in real beverages were experimentally demonstrated to be highly feasible by comparison with classical techniques like spectrophotometry, HPLC, and mass spectrometry.
Subject(s)
Food Coloring Agents , Beverages/analysis , Chromatography, High Pressure Liquid/methods , Food Coloring Agents/analysis , Mass Spectrometry , Solid Phase Extraction/methodsABSTRACT
Solid-state SERS sensors are desirable point-of-care tools due to their portability. However, the level of SERS sensitivity achieved in liquid phase is rarely duplicated in the solid phase. We report herein the fabrication of a SERS sensor using alumina beads as the solid support and demonstrate its high SERS sensitivity with the model analyte 4-aminophenyl disulfide (4-APDS). The key to sensitivity is a hydrophilic-hydrophobic surface gradient constructed by sequentially coating with the surfactant cetyltrimethylammonium bromide and fluorous 1H,1H,2H,2H-perfluorooctyltriethoxysilane. The surface gradient, together with chloride etching, allows the detection of 4-APDS at the low concentration of 10-15 M. The practicality of the sensor beads is evidenced by successfully tracking the SERS fingerprints of five food colorant standards in the SERS spectra of a popular candy product. These SERS sensor beads are easy to prepare, convenient to use, and highly responsive as a SERS platform for the analysis of colorants.
Subject(s)
Food Coloring Agents , Silver , Food Analysis , Food Coloring Agents/analysis , Hydrophobic and Hydrophilic Interactions , Silver/chemistry , Spectrum Analysis, RamanABSTRACT
Synthetic food colorants are extensively used across the globe regardless of the fact that they induce deleterious side effects when used in higher amounts. In this work, a novel electrochemical sensor based on nickel nanoparticles doped lettuce-like Co3O4 anchored graphene oxide (GO) nanosheets was developed for effective detection of sulfonated azo dye sunset yellow widely used as a food colorant. Hydrothermal synthesis was adopted for the preparation of lettuce-like spinel Co3O4 nanoparticles and Ni-Co3O4 NPs/GO nanocomposite was prepared using ecofriendly and economical sonochemical method. The prepared ternary nanocomposite meticulously fabricated on a screen-printed carbon electrode exhibited remarkable electrocatalytic activity towards sunset yellow determination. This is apparent from the resultant well-defined and intense redox peak currents of Ni-Co3O4 NPs/GO nanocomposite modified electrode at very low potentials. The developed sunset yellow sensor exhibited a high sensitivity of 4.16 µA µM-1 cm-2 and a nanomolar detection limit of 0.9 nM in the linear range 0.125-108.5 µM. Furthermore, experiments were conducted to affirm excellent stability, reproducibility, repeatability, and selectivity of proposed sensor. The practicality of sunset yellow determination using the developed sensor was analyzed in different varieties of food samples including jelly, soft drink, ice cream, and candy resulting in recovery in the range of 96.16%-102.56%.
Subject(s)
Azo Compounds/analysis , Electrochemical Techniques/methods , Food Coloring Agents/analysis , Metal Nanoparticles/chemistry , Nanocomposites/chemistry , Aluminum Oxide/chemistry , Cobalt/chemistry , Graphite , Limit of Detection , Linear Models , Magnesium Oxide/chemistry , Nickel/chemistry , Oxides/chemistry , Reproducibility of ResultsABSTRACT
In this study, a magnetic solid-phase extraction (MSPE) method coupled with High-Performance Liquid Chromatography Mass Spectrometry (HPLC-MS/MS) for the determination of illegal basic dyes in food samples was developed and validated. This method was based on Magnetic sulfonated reduced graphene oxide (M-S-RGO), which was sensitive and selective to analytes with structure of multiaromatic rings and negatively charged ions. Several factors affecting MSPE efficiency such as pH and adsorption time were optimized. Under the optimum conditions, the calibration curves exhibited good linearity, ranging from 5 to 60 µg/g with correlation coefficients >0.9950. The limits of detection of 16 basic dyes were in the range of 0.01-0.2 µg/L. The recoveries ranged from 70% to 110% with RSD% < 10%. The results indicate that M-S-RGO is an efficient and selective adsorbent for the extraction and cleanup of basic dyes. Due to the MSPE procedures, matrix effect and interference were eliminated in the analysis of HPLC-MS/MS without the matrix-matched standards. Thus, validation data showed that the proposed MSPE-HPLC-MS/MS method was rapid, efficient, selective, and sensitive for the determination of illegal basic dyes in foods.
Subject(s)
Food Analysis , Food Coloring Agents/analysis , Food Contamination/analysis , Graphite/chemistry , Solid Phase Extraction , Sulfonic Acids/chemistry , Chromatography, High Pressure Liquid , Magnetic Phenomena , Tandem Mass SpectrometryABSTRACT
There is growing evidence from human and animal studies indicating an association between exposure to synthetic food dyes and adverse neurobehavioral outcomes in children. However, data gaps persist for potential mechanisms by which the synthetic food dyes could elicit neurobehavioral impacts. We developed an approach to evaluate seven US FDA-batch-certified food dyes using publicly available high-throughput screening (HTS) data from the US EPA's Toxicity Forecaster to assess potential underlying molecular mechanisms that may be linked to neurological pathway perturbations. The dyes were screened through 270 assays identified based on whether they had a neurological-related gene target and/or were mapped to neurodevelopmental processes or neurobehavioral outcomes, and were conducted in brain tissue, targeted specific hormone receptors, or targeted oxidative stress and inflammation. Some results provided support for neurological impacts found in human and animal studies, while other results showed a lack of correlation with in vivo findings. The azo dyes had a range of activity in assays mapped to G-protein-coupled receptors and were active in assays targeting dopaminergic, serotonergic, and opioid receptors. Assays mapped to nuclear receptors (androgen, estrogen, and thyroid hormone) also exhibited activity with the food dyes. Other molecular targets included the aryl hydrocarbon receptor, acetylcholinesterase, and monoamine oxidase. The Toxicological Prioritization Index tool was used to visualize the results of the Novascreen assays. Our results highlight certain limitations of HTS assays but provide insight into potential underlying mechanisms of neurobehavioral effects observed in in vivo animal toxicology studies and human clinical studies.
Subject(s)
Food Analysis , Food Coloring Agents/analysis , High-Throughput Screening Assays , Animals , Food Coloring Agents/chemical synthesis , Food Coloring Agents/metabolism , Humans , Molecular Structure , Software , United States , United States Food and Drug AdministrationABSTRACT
Flowers of the genus Impatiens are classified as edible; however, their inclusion in the human diet is not yet a common practice. Its attractive colours have stirred great interest by the food industry. In this sense, rose (BP) and orange (BO) I. balsamina flowers were nutritionally studied, followed by an in-depth chemical study profile. The non-anthocyanin and anthocyanin profiles of extracts of both flower varieties were also determined by high-performance liquid chromatography coupled to a diode array and mass spectrometry detector (HPLC-DAD-ESI/MS). The results demonstrated that both varieties presented significant amounts of phenolic compounds, having identified nine non-anthocyanin compounds and 14 anthocyanin compounds. BP extract stood out in its bioactive properties (antioxidant and antimicrobial potential) and was selected for incorporation in "bombocas" filling. Its performance as a colouring ingredient was compared with the control formulations (white filling) and with E163 (anthocyanins) colorant. The incorporation of the natural ingredient did not cause changes in the chemical and nutritional composition of the product; and although the colour conferred was lighter than presented by the formulation with E163 (suggesting a more natural aspect), the higher antioxidant activity could meet the expectations of the current high-demand consumer.
Subject(s)
Anthocyanins , Food Coloring Agents/analysis , Impatiens , Plant Extracts/analysis , Anthocyanins/analysis , Antioxidants , Chromatography, High Pressure Liquid , FlowersABSTRACT
The use of artificial colorants in food is highly regulated due to their potential to harm human health. Thus, it is crucial to detect these substances effectively to ensure conformance with industrial standards. In this work, we prepared a photomultiplier tube (PMT)-based electrochemiluminescence (ECL) sensor and a charged coupled device (CCD)-based ECL sensor and compared their merits in the detection of sunset yellow (SY) dye. The sensors used C,N quantum dot-embedded g-C3N4 nanosheets (QDs@NSs) as the ECL agent and K2S2O8 as the coreactant. SY was analyzed on the basis of amplification in the QDs@NHs-K2S2O8 ECL system. The PMT-based sensor realized ultrasensitive detection using a single electrode, especially at low concentrations of SY. A CCD-based sensor imaged the ECL phenomenon of an electrode array and provided the advantages of high throughput and time savings. Under optimized conditions, both sensors exhibited high specificity, reproducibility and stability; detection limits of 20 nM with PMT detection and 5 µM with CCD detection were determined for SY, with detection ranging over at least two decades. The practical feasibilities of these systems were confirmed by satisfactory detection of SY in real drink samples.
Subject(s)
Azo Compounds/analysis , Carbonated Beverages/analysis , Electrochemical Techniques/instrumentation , Food Coloring Agents/analysis , Azo Compounds/chemistry , Electrochemical Techniques/methods , Electrodes , Food Coloring Agents/chemistry , Limit of Detection , Luminescent Measurements/instrumentation , Luminescent Measurements/methods , Nanostructures , Quantum Dots , Reproducibility of ResultsABSTRACT
Carmines (E120) are used worldwide as natural food colouring agents of animal origin, with a widespread application, including yoghurts. Despite being considered safe for human health, carmines are known to cause allergic reactions. Our goal was to evaluate the presence of carmines in different yoghurts with a label declaration of E120, purchased in Portugal, and, for the first time, to assess the human risk. The analytical methodology, recommended by JECFA, was based on acid hydrolysis of the samples followed by spectrophotometric UV-Vis analysis at 494 nm. This methodology allowed for a limit of quantification (LOQ) of 39.0 mg/kg and recovery rates higher than 97.7%. All the samples had carmines at levels above the LOQ, ranging between 43.8 and 193.8 mg/kg, with an average of 125.2 ± 34.5 mg/kg. In total, 8 (26.7%) samples exceeded the European Union (EU) maximum permitted level (MPL) established for carmines in this foodstuff, 150 mg/kg. Solid yoghurts presented higher average levels, 137.2 mg/kg, when compared to liquid samples, 107.2 mg/kg, with a significant statistical difference (p= 0.0236) being observed. No significant statistical difference was observed between white and private labels, whose average levels were very similar, 125.4 vs 125 mg/kg, respectively. Although some samples were above the allowable values, the estimated daily intake (EDI), designed for the different scenarios of different yoghurt types, did not exceed the established ADI, 5 mg/kg bw/day. According to the obtained results, carmine ingestion through the consumption of yoghurt poses low risk to the Portuguese consumers. However, children were the most vulnerable population group with a calculated risk value of up to 10% considering the mean content scenario. These first findings point out the need to reinforce surveillance programmes and monitoring studies, contributing to an increased awareness regarding carmine exposure, however it must be emphasised that yoghurt has evident nutritional benefits depending on a healthful consumer choice.