ABSTRACT
Copaifera langsdorffii L. is one of the most known medicinal species in Brazil. Its leaves are rich in phenolic compounds with potential biological activities as an antioxidant and chelating agent. This paper reports the isolation of four compounds from the hydroalcoholic extract of the leaves of C. langsdorffii and the investigation of their possible cytoprotective effects against heavy metal poisoning. Quercitrin (1), afzelin (2), 3,5-di-O-(3-O-methyl galloyl) quinic acid (3) and 4,5-di-O-(3-O-methyl galloyl) quinic acid (4), were associated with toxic doses of methylmercury and lead and evaluated by Alamar blue cell viability assays in HepG2 and PC12. The compounds displayed significant cytoprotective effect for the HepG2 cell line against both metals. Compounds 1-4 did not protect PC12 cells against methylmercury induced-cytotoxicity, but at lower concentrations, they protected against lead induced-cytotoxicity. The evaluated compounds showed a promising cytoprotection effect against exposure to heavy metals and should be further investigated as protective agents.
Subject(s)
Fabaceae/chemistry , Heavy Metal Poisoning/drug therapy , Methylmercury Compounds/antagonists & inhibitors , Plant Extracts/pharmacology , Protective Agents/isolation & purification , Animals , Antioxidants , Brazil , Cell Line , Heavy Metal Poisoning/prevention & control , Humans , Lead/toxicity , Lead Poisoning/drug therapy , Lead Poisoning/prevention & control , Mannosides , Mercury Poisoning/drug therapy , Mercury Poisoning/prevention & control , Methylmercury Compounds/toxicity , Phenols , Plant Leaves/chemistry , Proanthocyanidins , Protective Agents/pharmacology , Quercetin/analogs & derivatives , Quinic Acid , RatsABSTRACT
BACKGROUND: The present study aimed to evaluate the nutritional, physiological and biochemical effects of dietary supplementation of an association of probiotic bacteria in rats intoxicated with chromium (VI). Ninety-six male rats, recently weaned, were randomly divided into eight groups (n = 12): Control, DK12, DK24 and DK36 (0, 0.12, 0.24 and 0.36 g kg-1 of K2 Cr2 O7 incorporated in the basal feed, respectively) and groups Prob, DK12 + Prob, DK24 + Prob and DK36 + Prob received a progressive dose of 0, 0.12, 0.24 and 0.36 g kg-1 of K2 Cr2 O7 incorporated in the basal feed and supplemented with 0.02 g kg-1 of an association of probiotic bacteria (Lactobacillus acidophilus, Enterococcus faecium, Bifidobacterium thermophilum and Bifidobacterium longum). RESULTS: After 90 days, we observed significant (P < 0.05) and dose-dependent alterations from incorporation of increasing doses of chromium (VI) related to nutritional, physiological and biochemical parameters. These changes were attenuated (P < 0.05) with probiotic supplementation. CONCLUSION: Supplementation with probiotics in the diet beneficially modified the nutritional and physiological parameters, as well as hepatic, renal, glycemic and lipid profiles, of animals intoxicated with increasing doses of K2 Cr2 O7 . © 2018 Society of Chemical Industry.