ABSTRACT
Hepatitis is one of earlier, but serious, signs of liver damage. High doses of statins for a long time can induce hepatitis. This study aimed to evaluate and compare the therapeutic potential of thymoquinone (TQ) and bee pollen (BP) on fluvastatin (F)-induced hepatitis in rats. Rats were randomly divided into: group 1 (G1, control), G2 (F, hepatitis), G3 (F + TQ), G4 (F + BP), and G5 (F + TQ + BP). Single treatment with TQ or BP relieved fluvastatin-induced hepatitis, with best effect for the combined therapy. TQ and/or BP treatment significantly (1) reduced serum levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma glutamyl transpeptidase, and total bilirubin, (2) decreased malondialdehyde levels and increased level of reduced glutathione, and activities of glutathione peroxidase and catalase in the liver, (3) improved liver histology with mild deposition of type I collagen, (4) increased mRNA levels of transforming growth factor beta 1, nuclear factor Kappa B, and cyclooxygenase 1 and 2, and (5) decreased tumor necrosis factor alpha and upregulated interleukin 10 protein in the liver. These data clearly highlight the ability of TQ and BP combined therapy to cause better ameliorative effects on fluvastatin-induced hepatitis than individual treatment by each alone.
Subject(s)
Bees , Benzoquinones/pharmacology , Chemical and Drug Induced Liver Injury/drug therapy , Fluvastatin/adverse effects , Hepatitis, Animal/drug therapy , Pollen , Animals , Antioxidants/metabolism , Biomarkers , Chemical and Drug Induced Liver Injury/diagnosis , Chemical and Drug Induced Liver Injury/etiology , Chemical and Drug Induced Liver Injury/metabolism , Disease Management , Disease Susceptibility , Gene Expression , Hepatitis, Animal/diagnosis , Hepatitis, Animal/etiology , Hepatitis, Animal/metabolism , Immunohistochemistry , Liver Function Tests , Oxidative Stress/drug effects , Rats , Treatment OutcomeABSTRACT
In mammals, forkhead box O3 (foxo3) plays important roles in liver immune system. The foxo3 can regulate cell cycle, DNA repair, hypoxia, apoptosis and so on. However, as such an important transcription factor, few studies on foxo3 in fish have been reported. The present study characterized the foxo3 in turbot (Scophthalmus maximus L.). Lipopolysaccharide (LPS) incubated in vitro (hepatocytes) and injected in vivo (turbot liver) were used to construct inflammatory models. The foxo3 was interfered and overexpressed to investigate its functions in liver inflammation. The open reading frame (ORF) of foxo3 was 1998 bp (base pair), encoding 665 amino acids. Sequence analysis showed that foxo3 of turbot was highly homologous to other fishes. Tissue distribution analysis revealed that the highest expression of foxo3 was in muscle. Immunofluorescence result showed that foxo3 was expressed in cytoplasm and nucleus. Knockdown of foxo3 significantly increased mRNA levels of tumor necrosis factor-α (tnf-α), interleukin-1ß (il-1ß), interleukin-6 (il-6), myeloid-differentiation factor 88 (myd88), cd83, toll-like receptor 2 (tlr-2) and protein level of c-Jun N-terminal kinase (JNK) in sifoxo3 + LPS (siRNA of foxo3+ LPS) group compared with NC + LPS (negative control + LPS) group in turbot hepatocytes. Overexpressed foxo3 significantly decreased mRNA levels of tnf-α, il-6, nuclear transcription factor-kappa B (nf-κb), cd83, tlr-2 and the protein level of JNK in vitro. In vivo analysis, foxo3 knockdown significantly increased levels of GOT in serum after LPS injection compared with NC+LPS group. Overexpressed foxo3 significantly decreased levels of GPT and GOT in pcDNA3.1-foxo3+LPS group compared with pcDNA3.1+LPS group in vivo. Foxo3 knockdown significantly increased mRNA levels of tnf-α, il-1ß, il-6, nf-κb, myd88 and protein level of JNK in vivo in sifoxo3+LPS group compared with NC+LPS group in turbot liver. Overexpressed foxo3 significantly decreased mRNA levels of il-1ß, il-6, myd88, cd83, jnk and protein level of JNK in pcDNA3.1-foxo3+LPS group compared with pcDNA3.1+LPS group in turbot liver. The results indicated that foxo3 might modulate LPS-activated hepatic inflammation in turbot by decreasing the proinflammatory cytokines, the levels of GOT and GPT as well as activating JNK/caspase-3 and tlr-2/myd88/nf-κb pathways. Taken together, these findings indicated that FoxO3 may play important roles in liver immune responses to LPS in turbot and the research of FoxO3 in liver immunity enriches the studies on immune regulation, and provides theoretical basis and molecular targets for solving liver inflammation and liver injury in fish.
Subject(s)
Fish Diseases/etiology , Fish Diseases/metabolism , Forkhead Box Protein O3/metabolism , Hepatitis, Animal/etiology , Hepatitis, Animal/metabolism , Hepatocytes/metabolism , Lipopolysaccharides/adverse effects , Animals , Biomarkers , Cloning, Molecular , Disease Susceptibility , Fish Diseases/pathology , Flatfishes , Forkhead Box Protein O3/genetics , Gene Expression , Hepatitis, Animal/pathology , Hepatocytes/pathology , Liver Function Tests , RNA, Small InterferingABSTRACT
Traumatic reticuloperitonitis combined with embolic pneumonia and hepatitis is unusual signs of foreign body syndrome in cattle. A 4-year-old Holstein bull presented decreased appetite, dry cough, progressive weight loss, sternal recumbence and reluctance to stand and move. Laboratory tests revealed leucocytosis (18.4 × 103 /µl) accompanied by neutrophilia (10.48 × 103 /µl), and monocytosis (1.28 × 103 /µl), hyperglobulinaemia (6.3 g/dl), hypoalbuminaemia (1.5 g/dl), hyperfibrinogenaemia (10 g/L) and severe increase in gamma-glutamyl transferase activity (1,216 U/L). Reticular ultrasonographical examination showed a large amount of hyperechoic and hypoechoic content between the reticular serosa and the hepatic visceral surface. The main gross findings included fibrin deposition and adhesions between the reticulum, liver and diaphragm surfaces; a 4.0 mm in diameter transmural reticular perforation; a 12.0-cm diameter and scarce small randomly abscesses in the liver's parenchyma. The lungs presented multifocal areas of suppurative embolic foci (pulmonary abscesses), interstitial emphysema and multifocal fibrin deposition on the pleural surface. Ancillary diagnostic tests, such as ultrasonography and laboratory test, associated with clinical evaluation, may increase the accuracy of the correct diagnosis and avoid wasting time and money on untreatable cases.
Subject(s)
Cattle Diseases/diagnosis , Foreign Bodies/veterinary , Hepatitis, Animal/diagnosis , Peritonitis/veterinary , Pneumonia/veterinary , Animals , Cattle , Cattle Diseases/diagnostic imaging , Cattle Diseases/etiology , Foreign Bodies/complications , Hepatitis, Animal/diagnostic imaging , Hepatitis, Animal/etiology , Male , Peritonitis/diagnosis , Peritonitis/diagnostic imaging , Peritonitis/etiology , Pneumonia/diagnosis , Pneumonia/diagnostic imaging , Pneumonia/etiologyABSTRACT
A chronic high-fat diet (HFD) produces obesity, leading to pathological consequences in the liver and skeletal muscle. The fat in the liver leads to accumulation of a large number of intrahepatic lipid droplets (LD), which are susceptible to oxidation. Obesity also affects skeletal muscle, increasing LD and producing insulin signaling impairment. Physalis peruviana L. (PP) (Solanaceae) is rich in peruvioses and has high antioxidant activity. We assessed the ability of PP to enhance insulin-dependent glucose uptake in skeletal muscle and the capacity to prevent both inflammation and lipoperoxidation in the liver of diet-induced obese mice. Male C57BL/6J mice were divided into groups and fed for eight weeks: control diet (C; 10% fat, 20% protein, 70% carbohydrates); C + PP (300 mg/kg/day); HFD (60% fat, 20% protein, 20% carbohydrates); and HFD + PP. Results suggest that PP reduces the intracellular lipoperoxidation level and the size of LD in both isolated hepatocytes and skeletal muscle fibers. PP also promotes insulin-dependent skeletal muscle glucose uptake. In conclusion, daily consumption of 300 mg/kg of fresh pulp of PP could be a novel strategy to prevent the hepatic lipoperoxidation and insulin resistance induced by obesity.
Subject(s)
Hepatitis, Animal/etiology , Hepatitis, Animal/metabolism , Insulin Resistance , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Obesity/complications , Physalis/chemistry , Plant Extracts/pharmacology , Adipose Tissue/metabolism , Animals , Biomarkers , Body Weight , Diet, High-Fat , Disease Models, Animal , Fruit/chemistry , Glucose Tolerance Test , Hepatitis, Animal/pathology , Hepatitis, Animal/prevention & control , Inflammation Mediators/metabolism , Insulin/metabolism , Lipid Peroxidation/drug effects , Male , Mice , Obesity/etiology , Obesity/metabolism , Plant Extracts/chemistry , Protective Agents/chemistry , Protective Agents/pharmacologyABSTRACT
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD), a condition that leads to fibrosis, is caused by intake of very high-fat diets (HFDs). However, while the negative impact on the liver of these diets has been an issue of interest, systematic research on the effect of HFDs are lacking. OBJECTIVE: To characterize the overall impact of HFDs on both molecular and morphological signs of liver remodeling. METHODS: A study was conducted on male C57BL/6J mice to assess the effect of 4- and 8-week HFDs (60% kcal from fat) on (i) liver steatosis and fibrosis, and (ii) expression of factors involved in inflammation and angiogenesis. RESULTS: After an 8-week HFD, vascular endothelial growth factor type-2 receptor (VEGF-R2) and fatty acid translocase/trombospondin-1 receptor (CD36) were overexpressed in liver tissue of mice given HFDs. These changes suggest impaired liver angiogenesis and occurred together with (i) increased GPR78-BiP and EIF2α phosphorylation, suggesting endoplasmic reticulum stress, (ii) induction of Col1a1 gene expression, a marker of fibrosis, and (iii) increased CD31 immunolabeling, consistent with active angiogenesis and fibrosis. CONCLUSION: Our data show that very HFDs promote a rapid inflammatory response, as well as deregulation of angiogenesis, both consistent with development of liver fibrosis.
Subject(s)
Diet, High-Fat/adverse effects , Hepatitis, Animal/etiology , Neovascularization, Pathologic/etiology , Adiposity , Animals , Body Weight , Disease Models, Animal , Endoplasmic Reticulum Chaperone BiP , Endoplasmic Reticulum Stress , Gene Expression Regulation , Hepatitis, Animal/metabolism , Hepatitis, Animal/physiopathology , Inflammation Mediators/metabolism , Insulin/blood , Leptin/blood , Lipase/metabolism , Lipid Metabolism , Lipids/blood , Liver Cirrhosis/etiology , Liver Cirrhosis/metabolism , Liver Cirrhosis/physiopathology , Male , Mice , Mice, Inbred C57BL , Non-alcoholic Fatty Liver Disease/etiology , Non-alcoholic Fatty Liver Disease/metabolism , Non-alcoholic Fatty Liver Disease/physiopathologyABSTRACT
BACKGROUND/AIMS: The elaborate structure of the extracellular matrix (ECM) and the appropriate surface glycoforms upon it are indispensable to CD4+ T cell regulation. METHODS: To explore the effects of Glcα1,2Galß1 glycosylation mediated by GLT25D2 (Colgalt2) for CD4+ T cell regulation, we prepared C57BL/6J Glt25d2-/- mice. In the induction of hepatitis, after concanavalin A (Con A) challenge for 6, 12, and 24 h, more extensive parenchymal injury was noted in Glt25d2-/- mice than in wild-type (WT) mice at 12 h. Immunohistochemistry and laser scanning confocal microscopy were used to detect GLT25D2 expression, and subsets of CD4+T cells was analyzed by flow cytometry. A total of 26 cytokines in serum samples were determined using Luminex technology. RESULTS: The trend in liver injury score variation was consistent with serum alanine aminotransferase and aspartate aminotransferase levels. The levels of interleukin 4 (IL-4), IL-1ß, IL-9, and several chemokines such as macrophage inflammatory protein-2, eotaxin, and growth-related oncogene α were significantly increased in Glt25d2-/- mice compared with WT mice after Con A challenge. A further phenotype analysis of primary Glt25d2-/- CD4+ T cells showed that Glt25d2 knockout increased the frequency of the CD25+CD69- subset but decreased the frequency of the CD25-CD69+ subset after Con A challenge for 6, 12, and 24 h compared with those of WT CD4+ T cells. Activation-induced apoptosis was also significantly increased in Glt25d2-/- CD4+ T cells after Con A challenge compared with WT CD4+ T cells. Lectin microarray hybridization showed that Glt25d2 knockout increased the binding activity of Narcissus pseudonarcissus lectin to CD4+ T cells but Amaranthus caudatus lectin-binding activity was lost during Con A challenge. CONCLUSION: The present results suggest that collagen glycosylation mediated by GLT25D2 may regulate a subset of CD4+ T cells and be involved in the pathogenesis of Con A-induced hepatitis.
Subject(s)
CD4-Positive T-Lymphocytes/metabolism , Cell Proliferation/drug effects , Concanavalin A/pharmacology , Galactosyltransferases/genetics , Animals , Antigens, CD/metabolism , Antigens, Differentiation, T-Lymphocyte/metabolism , Apoptosis/drug effects , CD4-Positive T-Lymphocytes/cytology , CD4-Positive T-Lymphocytes/drug effects , Chemokines/blood , Cytokines/blood , Galactosyltransferases/deficiency , Hepatitis, Animal/etiology , Hepatitis, Animal/immunology , Hepatitis, Animal/pathology , Interleukin-2 Receptor alpha Subunit/metabolism , Lectins/metabolism , Lectins, C-Type/metabolism , Liver/metabolism , Male , Mice , Mice, Inbred C57BL , Mice, Knockout , Spleen/metabolismABSTRACT
To describe a case of naturally occurring pneumatosis intestinalis, pneumatosis coli and emphysematous hepatitis in a cat. A 9-month-old, indoors-only, female spayed, domestic medium hair cat presented for vomiting, open-mouth breathing and acute collapse. The initial physical examination identified moderate to severe hypothermia [35°C (95°F)], obtunded mentation, weak femoral pulses, tachycardia (heart rate 240 beats per min), pale pink mucous membranes and significant splenomegaly on abdominal palpation. Immediate diagnostics performed [packed cell volume and total solids (PCV, TS), venous blood gas and electrolytes] revealed severe anaemia (PCV 12%), hypoproteinaemia (TS = 2.2 g/dl), and severe metabolic acidosis (pH 6.956). Additional diagnostics performed included Feline Leukaemia Virus and Feline Immunodeficiency Virus testing (FeLV/FIV), complete blood count (CBC) with pathology review, serum biochemistry profile, prothrombin time (PT) and partial thromboplastin time (PTT), urinalysis, and abdominal radiographs. Abdominal radiographs were consistent with gas within hepatic and splenic veins and parenchyma, small intestinal walls and colonic wall. Due to the guarded prognosis, euthanasia was elected. Necropsy was performed and the most significant gross and histopathological findings included intra-luminal and intra-mural intestinal haemorrhage and vascular congestion with mild neutrophilic hepatitis, and marked hepatic periportal emphysema. Clostridium perfrigens and Escherichia coli were cultured from the bowel wall; no bacterial growth from the liver or spleen was identified. This case report describes idiopathic emphysematous hepatitis, with concurrent emphysema of the spleen and intestinal wall and intestinal haemorrhage. To the authors' knowledge, this type of pathology in a feline patient has not been previously described.
Subject(s)
Cat Diseases/diagnosis , Emphysema/veterinary , Hepatitis, Animal/etiology , Pneumatosis Cystoides Intestinalis/veterinary , Animals , Cat Diseases/diagnostic imaging , Cat Diseases/etiology , Cats , Diagnosis, Differential , Emphysema/diagnosis , Emphysema/diagnostic imaging , Emphysema/etiology , Female , Hepatitis, Animal/diagnosis , Hepatitis, Animal/diagnostic imaging , Intestines/diagnostic imaging , Intestines/pathology , Pneumatosis Cystoides Intestinalis/diagnosis , Pneumatosis Cystoides Intestinalis/diagnostic imaging , Pneumatosis Cystoides Intestinalis/etiology , Spleen/diagnostic imaging , Spleen/pathologyABSTRACT
Inclusion body hepatitis-hydropericardium syndrome (IBH-HPS) induced by fowl adenovirus type 4 (FAdV-4) has caused huge economic losses to the poultry industry of China, but the source of infection for different flocks, especially flocks with high biological safety conditions, has remained unclear. This study tested the pathogenicity of Newcastle disease virus (NDV)-attenuated vaccine from a large-scale poultry farm in China where IBH-HPS had appeared with high mortality. Analysis revealed that the NDV-attenuated vaccine in use from the abovementioned poultry farm was simultaneously contaminated with FAdV-4 and chicken infectious anemia virus (CIAV). The FAdV and CIAV isolated from the vaccine were purified for the artificial preparation of an NDV-attenuated vaccine singly contaminated with FAdV or CIAV, or simultaneously contaminated with both of them. Seven-day-old speciï¬c pathogen-free chicks were inoculated with the artificially prepared contaminated vaccines and tested for corresponding indices. The experiments showed that no hydropericardium syndrome (HPS) and corresponding death occurred after administering the NDV-attenuated vaccine singly contaminated with FAdV or CIAV, but a mortality of 75% with IBH-HPS was commonly found in birds after administering the NDV-attenuated vaccine co-contaminated with FAdV and CIAV. In conclusion, this study found the co-contamination of FAdV-4 and CIAV in the same attenuated vaccine and confirmed that such a contaminated attenuated vaccine was a significant source of infection for outbreaks of IBH-HPS in some flocks.
Subject(s)
Aviadenovirus/isolation & purification , Chicken anemia virus/isolation & purification , Drug Contamination , Inclusion Bodies/virology , Newcastle disease virus/genetics , Vaccines, Attenuated/adverse effects , Adenoviridae Infections/epidemiology , Adenoviridae Infections/etiology , Adenoviridae Infections/veterinary , Adenoviridae Infections/virology , Animals , Chickens/immunology , China/epidemiology , Circoviridae Infections/epidemiology , Circoviridae Infections/etiology , Circoviridae Infections/veterinary , Circoviridae Infections/virology , Hepatitis, Animal/epidemiology , Hepatitis, Animal/etiology , Hepatitis, Animal/virology , Newcastle Disease/epidemiology , Newcastle Disease/prevention & control , Newcastle Disease/virology , Newcastle disease virus/immunology , Poultry/immunology , Poultry/virology , Poultry Diseases/etiology , Poultry Diseases/virology , Specific Pathogen-Free Organisms , Syndrome , Vaccines, Attenuated/administration & dosage , Viral Vaccines/administration & dosage , Viral Vaccines/adverse effects , Viral Vaccines/immunologyABSTRACT
Rumen-derived lipopolysaccharide (LPS) is translocated from the rumen into the bloodstream when subacute ruminal acidosis (SARA) occurs following long-term feeding with a high-concentrate (HC) diet in dairy cows. The objective of this study was to investigate the mechanism of inflammatory responses in the liver caused by HC diet feeding. We found that SARA was induced in dairy cows when rumen pH below 5.6 lasted for at least 3 h/d with HC diet feeding. Also, the LPS levels in the portal and hepatic veins were increased significantly and hepatocytes were impaired as well as the liver function was inhibited during SARA condition. Meanwhile, the mRNA expression of immune genes including TNF receptor associated factor 6 (TRAF6), nuclear factor-kappa B (NF-κB), p38 mitogen-activated protein kinase (MAPK), extracellular regulated protein kinases (ERK) MAPK, Interleukin-1 (IL-1) and serum amyloid A (SAA) in the liver were significantly increased in SARA cows. Moreover, the phosphorylation level of NF-κB p65 and p38 MAPK proteins in the liver and the concentration of Tumor Necrosis Factor (TNF-α), Interleukin-1ß (IL-1ß) and Interleukin-6 (IL-6) in peripheral blood were obviously increased under SARA condition. In conclusion, the inflammatory injury in the liver caused by LPS that traveled from the digestive tract to the liver through the portal vein after feeding with a HC diet.
Subject(s)
Animal Feed , Hepatitis, Animal/etiology , Hepatitis, Animal/metabolism , Lipopolysaccharides/metabolism , Rumen/metabolism , Animals , Biomarkers , Cattle , Cytokines/blood , Cytokines/metabolism , Gene Expression Regulation , Hepatitis, Animal/blood , Hepatitis, Animal/pathology , Hepatocytes/metabolism , Hepatocytes/pathology , Hydrogen-Ion Concentration , Inflammation Mediators/metabolism , Lipopolysaccharides/blood , Liver Function Tests , NF-kappa B/metabolism , Phosphorylation , RNA, Messenger/genetics , RNA, Messenger/metabolism , Signal Transduction , p38 Mitogen-Activated Protein Kinases/metabolismABSTRACT
BACKGROUND & AIMS: The severity of liver diseases is exacerbated by the death of hepatocytes, which can be induced by the sensing of pathogen associated molecular patterns (PAMPs) derived from the gut microbiota. The molecular mechanisms regulating these cell death pathways are poorly documented. In this study, we investigated the role of the receptor interacting protein kinase 1 (RIPK1), a protein known to regulate cell fate decisions, in the death of hepatocytes using two in vivo models of PAMP-induced hepatitis. METHODS: Hepatitis was induced in mice by independent injections of two different bacterial PAMPs: lipopolysaccharide (LPS) and unmethylated CpG oligodeoxynucleotide (CpG-DNA) motifs. The role of RIPK1 was evaluated by using mice specifically lacking RIPK1 in liver parenchymal cells (Ripk1LPC-KO). Administration of liposome-encapsulated clodronate served to investigate the role of Kupffer cells in the establishment of the disease. Etanercept, a tumor necrosis factor (TNF)-decoy receptor, was used to study the contribution of TNF-α during LPS-mediated liver injury. RESULTS: Whereas RIPK1 deficiency in liver parenchymal cells did not trigger basal hepatolysis, it greatly sensitized hepatocytes to apoptosis and liver damage following a single injection of LPS or CpG-DNA. Importantly, hepatocyte death was prevented by previous macrophage depletion or by TNF inhibition. CONCLUSIONS: Our data highlight the pivotal function of RIPK1 in maintaining liver homeostasis in conditions of macrophage-induced TNF burst in response to PAMPs sensing. LAY SUMMARY: Excessive death of hepatocytes is a characteristic of liver injury. A new programmed cell death pathway has been described involving upstream death ligands such as TNF and downstream kinases such as RIPK1. Here, we show that in the presence of LPS liver induced hepatic injury was due to secretion of TNF by liver macrophages, and that RIPK1 acts as a powerful protector of hepatocyte death. This newly identified pathway in the liver may be helpful in the management of patients to predict their risk of developing acute liver failure.
Subject(s)
Hepatitis, Animal/metabolism , Hepatitis, Animal/pathology , Hepatocytes/metabolism , Hepatocytes/pathology , Kupffer Cells/metabolism , Kupffer Cells/pathology , Pathogen-Associated Molecular Pattern Molecules/toxicity , Receptor-Interacting Protein Serine-Threonine Kinases/metabolism , Tumor Necrosis Factor-alpha/metabolism , Animals , Apoptosis/drug effects , Hepatitis, Animal/etiology , Hepatocytes/drug effects , Kupffer Cells/drug effects , Lipopolysaccharides/toxicity , Mice , Mice, Knockout , Receptor-Interacting Protein Serine-Threonine Kinases/deficiency , Receptor-Interacting Protein Serine-Threonine Kinases/geneticsABSTRACT
The World Small Animal Veterinary Association's Liver Standardization Group produced standardized criteria for the histologic diagnosis of canine chronic hepatitis (CH). They define CH by the presence of hepatocellular apoptosis or necrosis, a variable mononuclear or mixed inflammatory cell infiltrate, regeneration, and fibrosis. There are variations in histologic appearance between breeds. Hepatic copper accumulation is an important cause of canine CH. However, where copper accumulation has been ruled out, dogs are said to have idiopathic CH. This article reviews theories regarding the etiopathogenesis of canine CH other than copper accumulation, and its clinical features, diagnostic findings, and management.
Subject(s)
Dog Diseases , Hepatitis, Animal , Hepatitis, Chronic/veterinary , Animals , Antifibrinolytic Agents/therapeutic use , Diet Therapy/veterinary , Dog Diseases/diagnosis , Dog Diseases/etiology , Dog Diseases/physiopathology , Dog Diseases/therapy , Dogs , Female , Hepatitis, Animal/diagnosis , Hepatitis, Animal/etiology , Hepatitis, Animal/physiopathology , Hepatitis, Animal/therapy , Hepatitis, Chronic/etiology , Hepatitis, Chronic/physiopathology , Hepatitis, Chronic/therapy , Male , Prognosis , Risk Factors , Ursodeoxycholic Acid/therapeutic useABSTRACT
cAMP responsive element binding protein 3-like 3 (CREB3L3), a transcription factor expressed in the liver and small intestine, governs fasting-response energy homeostasis. Tissue-specific CREB3L3 knockout mice have not been generated till date. To our knowledge, this is the first study using the one-step CRISPR/Cas9 system to generate CREB3L3 floxed mice and subsequently obtain liver- and small intestine-specific Creb3l3 knockout (LKO and IKO, respectively) mice. While LKO mice as well as global KO mice developed hypertriglyceridemia, LKO mice exhibited hypercholesterolemia in contrast to hypocholesterolemia in global KO mice. LKO mice demonstrated up-regulation of hepatic Srebf2 and its corresponding target genes. No phenotypic differences were observed between IKO and floxed mice. Severe liver injury was observed in LKO mice fed a methionine-choline deficient diet, a model for non-alcoholic steatohepatitis. These results provide new evidence regarding the hepatic CREB3L3 role in plasma triglyceride metabolism and hepatic and intestinal CREB3L3 contributions to cholesterol metabolism.
Subject(s)
CRISPR-Cas Systems/genetics , Cyclic AMP Response Element-Binding Protein/genetics , Hepatitis, Animal/etiology , Hyperlipidemias/etiology , Liver/metabolism , Animals , Cholesterol/metabolism , Choline Deficiency , Cyclic AMP Response Element-Binding Protein/deficiency , Female , Hepatitis, Animal/metabolism , Hyperlipidemias/metabolism , Hyperlipidemias/veterinary , Insulin/blood , Intestine, Small/metabolism , Liver/pathology , Male , Methionine/deficiency , Mice , Mice, Inbred C57BL , Mice, Knockout , Non-alcoholic Fatty Liver Disease/etiology , Non-alcoholic Fatty Liver Disease/metabolism , Sterol Regulatory Element Binding Protein 2/metabolism , Triglycerides/blood , Triglycerides/metabolism , Up-RegulationABSTRACT
Cell ablation is a powerful tool for studying cell lineage and/or function; however, current cell-ablation models have limitations. Intermedilysin (ILY), a cytolytic pore-forming toxin that is secreted by Streptococcus intermedius, lyses human cells exclusively by binding to the human complement regulator CD59 (hCD59), but does not react with CD59 from nonprimates. Here, we took advantage of this feature of ILY and developed a model of conditional and targeted cell ablation by generating floxed STOP-CD59 knockin mice (ihCD59), in which expression of human CD59 only occurs after Cre-mediated recombination. The administration of ILY to ihCD59+ mice crossed with various Cre-driver lines resulted in the rapid and specific ablation of immune, epithelial, or neural cells without off-target effects. ILY had a large pharmacological window, which allowed us to perform dose-dependent studies. Finally, the ILY/ihCD59-mediated cell-ablation method was tested in several disease models to study immune cell functionalities, hepatocyte and/or biliary epithelial damage and regeneration, and neural cell damage. Together, the results of this study demonstrate the utility of the ihCD59 mouse model for studying the effects of cell ablation in specific organ systems in a variety of developmental and disease states.
Subject(s)
Bacteriocins/administration & dosage , CD59 Antigens/physiology , Animals , Astrocytes/drug effects , Astrocytes/immunology , Astrocytes/pathology , Bacteriocins/toxicity , CD59 Antigens/genetics , Gene Knock-In Techniques , Hemolysis/genetics , Hemolysis/physiology , Hepatitis, Animal/etiology , Hepatitis, Animal/genetics , Hepatitis, Animal/immunology , Hepatocytes/drug effects , Hepatocytes/immunology , Hepatocytes/pathology , Humans , Integrases , Lymphocyte Depletion , Mice , Mice, Inbred C57BL , Mice, Transgenic , T-Lymphocytes/drug effects , T-Lymphocytes/immunology , T-Lymphocytes/pathologyABSTRACT
Common parenchymal liver diseases in dogs include reactive hepatopathies and primary hepatitis (acute or chronic). In chronic hepatitis, there is usually a long subclinical phase. Specific clinical signs become overt only when liver damage is severe and in this phase, treatment is usually less effective. Limited data are available regarding the sensitivity of liver enzyme activity or biomarkers for early detection of subclinical hepatitis. Hepatocyte-derived microRNAs (HDmiRs) were recently identified as promising biomarkers for hepatocellular injury in multiple species. Here, the potential of the HDmiRs miR-122 and miR-148a as sensitive diagnostic biomarkers for hepatocellular injury in Labrador retrievers was investigated. Samples from 66 Labrador retrievers with histologically normal livers, high hepatic copper, and with various forms of liver injury were evaluated for serum alanine aminotransferase (ALT) activity and microRNA values. Median values of HDmiR-122 were 34.6 times higher in dogs with liver injury and high ALT than in normal dogs (95% confidence intervals [CI], 13-95; P <0.001). HDmiR-122 values were significantly increased in dogs with liver injury and normal ALT (4.2 times; 95% CI, 2-12; P <0.01) and in dogs with high hepatic copper concentrations and unremarkable histopathology (2.9 times; 95% CI, 1.1-8.0; P <0.05). Logistic regression analyses demonstrated that miR-122 and miR-148a were both predictors of hepatocellular injury. The sensitivity of miR-122 was 84% (95% CI, 73-93%), making it superior to ALT (55%; 95% CI, 41-68%) for the detection of hepatocellular injury in Labrador retrievers (P <0.001). This study demonstrated that serum HDmiR, particularly miR-122, is a highly sensitive marker for the detection of hepatocellular injury in Labrador retrievers and is a promising new biomarker that may be used for early detection of subclinical hepatitis in dogs.
Subject(s)
Alanine Transaminase/blood , Chemical and Drug Induced Liver Injury/veterinary , Copper/toxicity , Dog Diseases/diagnosis , Hepatitis, Animal/diagnosis , MicroRNAs/blood , Animals , Biomarkers/blood , Chemical and Drug Induced Liver Injury/diagnosis , Chemical and Drug Induced Liver Injury/etiology , Dog Diseases/etiology , Dogs , Female , Hepatitis, Animal/etiology , Hepatocytes/metabolism , MaleABSTRACT
Disruption of the mucosal epithelium during lentivirus infections permits translocation of microbial products into circulation, causing immune activation and driving disease. Although the liver directly filters blood from the intestine and is the first line of defense against gut-derived antigens, the effects of microbial products on the liver are unclear. In livers of normal macaques, minute levels of bacterial products were detectable, but increased 20-fold in simian immunodeficiency virus (SIV)-infected animals. Increased microbial products in the liver induced production of the chemoattractant CXCL16 by myeloid dendritic cells (mDCs), causing subsequent recruitment of hypercytotoxic natural killer (NK) cells expressing the CXCL16 receptor, CXCR6. Microbial accumulation, mDC activation, and cytotoxic NK cell frequencies were significantly correlated with markers of liver damage, and SIV-infected animals consistently had evidence of hepatitis and fibrosis. Collectively, these data indicate that SIV-associated accumulation of microbial products in the liver initiates a cascade of innate immune activation, resulting in liver damage.
Subject(s)
Killer Cells, Natural/physiology , Liver/metabolism , Myeloid Cells/physiology , Simian Acquired Immunodeficiency Syndrome/complications , Simian Immunodeficiency Virus , Animals , Apoptosis , Chemokines, CXC/genetics , Chemokines, CXC/metabolism , Gene Expression Regulation , Hepatitis, Animal/etiology , Hepatitis, Animal/metabolism , Hepatitis, Animal/pathology , Liver/microbiology , Macaca mulatta , Receptors, CXCR/genetics , Receptors, CXCR/metabolism , Simian Acquired Immunodeficiency Syndrome/metabolism , Simian Acquired Immunodeficiency Syndrome/microbiologyABSTRACT
A 7-month-old female domestic shorthair cat was diagnosed with chronic regenerative hemolytic anemia characterized by increased osmotic fragility of unknown etiology. At 13 months of age, the cat was evaluated for acute collapse. The cat was icteric with severe hyperbilirubinemia but no hematocrit changes. Severe obtundation and lateral recumbency progressed to tetraparesis and loss of proprioception in all 4 limbs, and a cerebellar or brainstem lesion was suspected. Postmortem examination revealed suppurative cholangiohepatitis and acute neuronal necrosis in the nuclei of the brainstem and cerebellum, consistent with bilirubin encephalopathy. This is the first known occurrence of cholangiohepatitis and bilirubin encephalopathy in an adult cat with chronic hemolytic anemia. Although rare, bilirubin encephalopathy should be considered a possible sequela to hyperbilirubinemia in adult patients. It remains unknown whether increased osmotic fragility was related to the cholangiohepatopathy.
Subject(s)
Anemia, Hemolytic/veterinary , Cat Diseases/diagnosis , Cholangitis/veterinary , Hepatitis, Animal/etiology , Kernicterus/veterinary , Anemia, Hemolytic/etiology , Animals , Bile Ducts/pathology , Cat Diseases/etiology , Cat Diseases/pathology , Cats , Cholangitis/diagnosis , Cholangitis/pathology , Female , Hyperbilirubinemia , Kernicterus/diagnosis , Kernicterus/pathology , Liver/pathology , Osmotic FragilityABSTRACT
The objective of this study was to identify the causative agents of hepatitis observed in broiler chickens at processing. Livers of chickens from 16 broiler farms in Saskatchewan with gross lesions of hepatitis were collected at processing. In addition to routine bacterial isolation and histopathological examination, serologic studies for infectious bursal disease virus (IBDV) and Chicken anaemia virus (CAV), calculation of the ratio of the weight of the bursa of Fabricius (BF) to body weight (BBW), and histopathological examination of the BF were done. Of the 264 livers with gross lesions, 83% had multifocal to coalescing necrotizing hepatitis, 16% had perihepatitis, and 1% had hemorrhages. No definitive causative microorganisms were isolated from the hepatic lesions; however, no significant bacterial isolations were made. Bursal atrophy, low BBW ratio, and high titer of antibody against IBDV each correlated with the rate of total condemnations (P = 0.0188, P = 0.0001, and P = 0.0073, respectively). Nucleotide sequencing of IBDV isolated from the BF identified the variant strains Delaware-E and 586. Condemnation because of hepatic lesions was correlated with titer of antibody against IBDV and BBW (P = 0.016 and P = 0.027). The results of this study demonstrate that hepatic lesions in Saskatchewan chickens are not currently caused by a primary bacterial pathogen but are associated with indicators of immunosuppression that is likely due to variant IBDV.
L'objectif de la présente étude était d'identifier les agents causals de l'hépatite observée chez des poulets à griller au moment de la transformation. Les foies de poulets provenant de 16 fermes de poulets à griller en Saskatchewan avec des lésions macroscopiques d'hépatite furent prélevés. En plus de l'isolement bactérien de routine et de l'examen histopathologique, on effectua des analyses sérologiques pour le virus de la bursite infectieuse aviaire (VBIA) et le virus de l'anémie du poulet (VAP), le calcul du ratio du poids de la bourse de Fabricius (BF) sur le poids corporel (BPC), et l'examen histopathologique de la BF. Sur les 264 foies ayant des lésions macroscopiques, 83 % avaient des lésions multifocales à coalescentes d'hépatite nécrosante, 16 % de la péri-hépatite et 1 % des hémorragies. Aucun agent causal définitif ne fut isolé des lésions hépatiques; toutefois, aucun agent bactérien significatif ne fut isolé. Une atrophie de la bourse, un faible ratio BPC, et un titre élevé d'anticorps dirigé contre VBIA corrélaient tous avec le taux de condamnation totale (P = 0,0188, P = 0,0001, et P = 0,0073, respectivement). Le séquençage nucléotidique des VBIA isolés des BF identifia les souches variantes Delaware-E et 586. La condamnation due aux lésions hépatiques était corrélée avec le titre d'anticorps contre VBIA et le BPC (P = 0,016 et P = 0,027, respectivement). Les résultats de la présente étude démontrent que les lésions hépatiques chez les poulets de la Saskatchewan ne sont pas actuellement causées par un agent bactérien pathogène primaire mais sont associées à des indicateurs d'immunosuppression qui est probablement causée par un variant de VBIA.(Traduit par Docteur Serge Messier).
Subject(s)
Chickens , Hepatitis, Animal/pathology , Poultry Diseases/pathology , Animals , Antibodies, Viral/blood , Bacterial Infections/complications , Bacterial Infections/epidemiology , Bacterial Infections/microbiology , Bacterial Infections/veterinary , Birnaviridae Infections/complications , Birnaviridae Infections/veterinary , Chicken anemia virus , Circoviridae Infections/complications , Circoviridae Infections/veterinary , Hepatitis, Animal/epidemiology , Hepatitis, Animal/etiology , Infectious bursal disease virus , Poultry Diseases/epidemiology , Poultry Diseases/etiology , Saskatchewan/epidemiology , Serologic TestsABSTRACT
Pancreatitis in cats is frequently accompanied by concurrent disease in other organ systems. Co-morbidities include hepatic lipidosis, inflammatory liver disease, bile duct obstruction, diabetes mellitus, inflammatory bowel disease, vitamin deficiency (B12/cobalamin, folate or K), intestinal lymphoma, nephritis, pulmonary thromboembolism and pleural and peritoneal effusions. "Triaditis" is the term used to describe concurrent inflammation of the pancreas, liver and small intestines. Triaditis has been reported in 50 to 56% of cats diagnosed with pancreatitis and 32 to 50% of those with cholangitis/inflammatory liver disease. A definitive diagnosis of triaditis is based on the histopathological evaluation of each organ. However, the specific conditions of each organ that constitute a diagnosis of triaditis remains to be defined. While the aetiopathogenesis of pancreatitis and its relationship to inflammation in other organ systems is unclear, preliminary studies point to a heterogeneous group of conditions with differential involvement of host inflammatory and immune responses and enteric bacteria. Comprehensive, prospective studies that simultaneously evaluate the presence of predefined clinical, clinicopathological and histopathological abnormalities, coupled with high-resolution evaluation of pancreaticobiliary morphology, immunological profiling and screening for bacterial colonisation are required to advance diagnosis and therapy.
Subject(s)
Cat Diseases/etiology , Enteritis/veterinary , Hepatitis, Animal/complications , Pancreatitis/veterinary , Animals , Cat Diseases/therapy , Cats , Enteritis/complications , Enteritis/etiology , Enteritis/therapy , Hepatitis, Animal/etiology , Hepatitis, Animal/therapy , Pancreatitis/etiology , Pancreatitis/therapySubject(s)
Angiomatosis, Bacillary/veterinary , Bartonella henselae , Cholangitis/veterinary , Hepatitis, Animal/microbiology , Horse Diseases/microbiology , Liver/microbiology , Angiomatosis, Bacillary/complications , Angiomatosis, Bacillary/pathology , Animals , Cholangitis/etiology , Cholangitis/microbiology , Hepatitis, Animal/etiology , Hepatitis, Animal/pathology , Horse Diseases/pathology , Horses/microbiology , Liver/pathology , MaleABSTRACT
The One Health principle recognizes that human health, animal health, and environmental health are inextricably linked. An excellent example is the study of naturally occurring copper toxicosis in dogs to help understand human disorders of copper metabolism. Besides the Bedlington terrier, where copper toxicosis is caused by a mutation in the COMMD1 gene, more complex hereditary forms of copper-associated hepatitis were recognized recently in other dog breeds. The Labrador retriever is one such breed, where an interplay between genetic susceptibility and exposure to copper lead to clinical copper toxicosis. Purebred dog populations are ideal for gene mapping studies, and because genes involved in copper metabolism are highly conserved across species, newly identified gene mutations in the dog may help unravel the genetic complexity of different human forms of copper toxicosis. Furthermore, increasing knowledge with respect to diagnosis and treatment strategies will benefit both species.