ABSTRACT
The search for more effective platinum anticancer drugs has led to the design, synthesis, and preclinical testing of hundreds of new platinum complexes. This search resulted in the recognition and subsequent FDA approval of the third-generation Pt(II) anticancer drug, [Pt(1,2-diaminocyclohexane)(oxalate)], oxaliplatin, as an effective agent in treating colorectal and gastrointestinal cancers. Another promising example of the class of anticancer platinum(II) complexes incorporating the Pt(1,n-diaminocycloalkane) moiety is kiteplatin ([Pt(cis-1,4-DACH)Cl2], DACH = diaminocyclohexane). We report here our progress in evaluating the role of the cycloalkyl moiety in these complexes focusing on the synthesis, characterization, evaluation of the antiproliferative activity in tumor cells and studies of the mechanism of action of new [Pt(cis-1,3-diaminocycloalkane)Cl2] complexes wherein the cis-1,3-diaminocycloalkane group contains the cyclobutyl, cyclopentyl, and cyclohexyl moieties. We demonstrate that [Pt(cis-1,3-DACH)Cl2] destroys cancer cells with greater efficacy than the other two investigated 1,3-diamminocycloalkane derivatives, or cisplatin. Moreover, the investigated [Pt(cis-1,3-diaminocycloalkane)Cl2] complexes show selectivity toward tumor cells relative to non-tumorigenic normal cells. We also performed several mechanistic studies in cell-free media focused on understanding some early steps in the mechanism of antitumor activity of bifunctional platinum(II) complexes. Our data indicate that reactivities of the investigated [Pt(cis-1,3-diaminocycloalkane)Cl2] complexes and cisplatin with glutathione and DNA binding do not correlate with antiproliferative activity of these platinum(II) complexes in cancer cells. In contrast, we show that the higher antiproliferative activity in cancer cells of [Pt(cis-1,3-DACH)Cl2] originates from its highest hydrophobicity and most efficient cellular uptake.
Subject(s)
Antineoplastic Agents/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Organometallic Compounds/chemical synthesis , Platinum/chemistry , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Cell Line, Tumor , Cell Membrane Permeability , Cell Proliferation/drug effects , Cisplatin/pharmacology , Cisplatin/standards , DNA/chemistry , Drug Screening Assays, Antitumor , Glutathione/chemistry , Humans , Organometallic Compounds/pharmacologyABSTRACT
RORγt is the master regulator of the IL-23/IL-17 axis, a pathway that is clinically validated for the treatment of various immunological disorders. Over the last few years, our group has reported different chemotypes that potently act as inverse agonists of RORγt. One of them, the tricyclic pyrrolidine chemotype, has demonstrated biologic-like preclinical efficacy and has led to our clinical candidate BMS-986251. In this letter, we discuss the invention of an annulation reaction which enabled the synthesis of a tricyclic exocyclic amide chemotype and the identification of compounds with RORγt inverse agonist activity. Preliminary structure activity relationships are disclosed.
Subject(s)
Amides/chemistry , Hydrocarbons, Cyclic/chemistry , Nuclear Receptor Subfamily 1, Group F, Member 3/antagonists & inhibitors , Sulfones/chemistry , Amides/chemical synthesis , Amides/metabolism , Animals , Cyclization , Drug Inverse Agonism , Humans , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/metabolism , Mice , Microsomes, Liver/metabolism , Molecular Docking Simulation , Molecular Structure , Nuclear Receptor Subfamily 1, Group F, Member 3/metabolism , Structure-Activity Relationship , Sulfones/chemical synthesis , Sulfones/metabolismABSTRACT
Ring systems in pharmaceuticals, agrochemicals, and dyes are ubiquitous chemical motifs. While the synthesis of common ring systems is well described and novel ring systems can be readily and computationally enumerated, the synthetic accessibility of unprecedented ring systems remains a challenge. "Ring Breaker" uses a data-driven approach to enable the prediction of ring-forming reactions, for which we have demonstrated its utility on frequently found and unprecedented ring systems, in agreement with literature syntheses. We demonstrate the performance of the neural network on a range of ring fragments from the ZINC and DrugBank databases and highlight its potential for incorporation into computer aided synthesis planning tools. These approaches to ring formation and retrosynthetic disconnection offer opportunities for chemists to explore and select more efficient syntheses/synthetic routes.
Subject(s)
Chemistry Techniques, Synthetic/methods , Heterocyclic Compounds/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Neural Networks, Computer , Databases, ChemicalABSTRACT
Natural products containing eight-membered carbocycles constitute a class of structurally intriguing and biologically important molecules such as the famous diterpenes taxol and vinigrol. Such natural products are being increasingly investigated because of their fascinating architectural features and potent medicinal properties. However, synthesis of natural products with cyclooctane moieties has proved to be highly challenging. This review highlights the recently completed total syntheses of natural products with eight-membered carbocycles with a focus on strategic considerations. A collection of 27 representative studies from the literature covering the decade from 2009 to 2019 is described in chronological order with relevant studies grouped together, including syntheses of the same natural product by different research groups using different strategies. Finally, a summary and outlook including a discussion of the major features of each strategy used in the syntheses are presented. This review illustrates the diversity and creativity in the elegant synthetic designs of eight-membered carbocycles. We hope this review will provide timely illumination and beneficial guidance for future synthetic efforts for organic chemists who are interested in this area.
Subject(s)
Biological Products/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Biological Products/chemistry , Cyclization , Hydrocarbons, Cyclic/chemistry , Molecular ConformationABSTRACT
A key challenge in the synthesis of diterpenoid alkaloids lies in identifying strategies that rapidly construct their multiply bridged polycyclic skeletons. Existing approaches to these structurally intricate secondary metabolites are discussed in the context of a "bond-network analysis" of molecular frameworks, which was originally devised by Corey some 40 years ago. The retrosynthesis plans that emerge from a topological analysis of the highly bridged frameworks of the diterpenoid alkaloids are discussed in the context of eight recent syntheses of hetidine and hetisine natural products and their derivatives. This Minireview highlights the extent to which network analyses of the type described here sufficed for designing synthesis plans, as well as areas where they had to be amalgamated with functional group oriented synthetic planning considerations.
Subject(s)
Alkaloids/chemical synthesis , Diterpenes/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Alkaloids/chemistry , Diterpenes/chemistry , Hydrocarbons, Cyclic/chemistry , Hydrogen Bonding , Molecular StructureABSTRACT
BACKGROUND: One of the most successful reagents used in the synthesis of the reactive enaminone is DMF-DMA, but it is very expensive with harmful effects on the human health and reacts with special compounds to generate the enaminone such as active methylene centers. AIM: In this article, we synthesized a new ketenaminal by simple method with inexpensive reagents (through desulfurization in diphenylether). METHODS: Thus, a novel reactive ketenaminal (enaminone) was synthesized from the desulfurization of 2-((2-(4-chlorophenyl)-2-oxoethyl)thio)-5,7-bis(4-methoxyphenyl)pyrido[2,3-d]pyrimidin- 4(3H)-one with diphenylether. The starting keteneaminal was coupled with diazotized anilines via the known coupling conditions to give a new series of 2-(4-chlorophenyl)-1-(2-(arylhydrazono)-2- oxoethyl)-5,7-bis(4-methoxy-phenyl)pyrido[2,3-d]pyrimidin-4(1H)-ones. RESULTS: The structures of the new compounds were elucidated based on their IR, 1H-NMR, 13CNMR, and Mass spectra. Moreover, the potency of these compounds as antimicrobial agents has been evaluated. The results showed that some of the products have high activity nearly equal to that of the used standard antibiotic. Additionally, the docking study was done to get the binding mode of the synthesized compounds with the binding site of the DHFR enzyme. The results of molecular docking of the synthesized arylhydrazono compounds are able to fit in DHFR binding site with binding energies ranging from -4.989 to -8.178 Kcal/mol. CONCLUSION: Our goal was achieved in this context by the synthesis of new ketenaminal from inexpensive reagents, which was utilized in the preparation of bioactive arylhydrazone derivatives.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Antifungal Agents/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/pharmacology , Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Aspergillus fumigatus/drug effects , Bacteria/drug effects , Candida albicans/drug effects , Hydrocarbons, Cyclic/chemistry , Models, Molecular , Molecular Docking Simulation , Molecular Structure , Structure-Activity RelationshipABSTRACT
Here, we demonstrate that a metallaphotoredox-catalyzed cross-electrophile coupling mechanism provides a unified method for the α-arylation of diverse activated alkyl chlorides, including α-chloroketones, α-chloroesters, α-chloroamides, α-chlorocarboxylic acids, and benzylic chlorides. This strategy, which is effective for a wide variety of aryl bromide coupling partners, is predicated upon a halogen atom abstraction/nickel radical-capture mechanism that is generically successful across an extensive range of carbonyl substrates. The construction and use of arylacetic acid products have further enabled two-step protocols for the delivery of valuable building blocks for medicinal chemistry, such as aryldifluoromethyl and diarylmethane motifs.
Subject(s)
Hydrocarbons, Cyclic/chemical synthesis , Metals/chemistry , Catalysis , Hydrocarbons, Cyclic/chemistry , Hydrogen Bonding , Molecular Structure , Oxidation-ReductionABSTRACT
Five new dibenzocyclooctynes bearing different substituents on their aryl moieties were synthesized and evaluated for their reactivity toward strain-promoted alkyne-azide cycloaddition (SPAAC). The dinaphthylcyclooctynes proved to be poorly reactive with azides, and the formation of triazole required many days compared to a few hours for the other cyclooctynes. Fluoride atoms and methoxy groups were also introduced to the aryl rings, leading to more active compounds. Oxidation of the alcohol on the cyclooctyne ring also increased the reaction rates by 3.5- to 6-fold. 3,9-Difluoro-4,8-dimethoxy-dibenzocyclooctyne-1-one thus displayed a SPAAC kinetic rate of 3.5 M-1 s-1, which is one of the highest rates ever described. Furthermore, the dibenzocyclooctyn-1-one displayed fluorescence properties that have allowed their detection in the protozoan parasites Plasmodium falciparum and Trypanosoma brucei by microscopy imaging, proving that they can cross cell membranes and that they are stable enough in biological media.
Subject(s)
Hydrocarbons, Cyclic/chemical synthesis , Antiprotozoal Agents/chemical synthesis , Antiprotozoal Agents/chemistry , Antiprotozoal Agents/pharmacology , Azides , Cycloaddition Reaction , Fluorescence , Hydrocarbons, Cyclic/chemistry , Hydrocarbons, Cyclic/metabolism , Molecular Structure , Plasmodium falciparum/metabolism , Trypanosoma brucei brucei/metabolismABSTRACT
Several novel cycloalkyl-fused 2,3-diaryl pyrazole derivatives were designed, synthesized, and evaluated as potential anti-tubulin agents. Compound A10 exhibited the most potent antiproliferative activity against a panel of cancer lines (IC50 = 0.78-2.42 µM) and low cytotoxicity against 293T & L02 (CC50 values of 131.74 and 174.89 µM, respectively). Moreover, A10 displayed inhibition of tubulin polymerization in vitro, arrested the G2/M phase of the cell cycle, changed morphology of tubulin, increased intracellular reactive oxygen species, and induced apoptosis of HeLa cells. Docking simulation and 3D-QSAR models were performed to elaborate on the anti-tubulin mechanism of the derivatives. The inhibition of monoclonal colony formation provided more intuitional data to verify the possibility of A10 as a novel tubulin assembling inhibitor.
Subject(s)
Antineoplastic Agents/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/metabolism , Pyrazoles/chemical synthesis , Tubulin Modulators/chemical synthesis , Tubulin/metabolism , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Drug Screening Assays, Antitumor , Humans , Hydrocarbons, Cyclic/chemistry , Molecular Docking Simulation , Molecular Structure , Protein Binding , Protein Conformation , Pyrazoles/pharmacology , Quantitative Structure-Activity Relationship , Reactive Oxygen Species/metabolism , Tubulin Modulators/pharmacologyABSTRACT
Nonconjugated hydrocarbons, like bicyclo[1.1.1]pentane, bicyclo[2.2.2]octane, triptycene, and cubane are a unique class of rigid linkers. Due to their similarity in size and shape they are useful mimics of classic benzene moieties in drugs, so-called bioisosteres. Moreover, they also fulfill an important role in material sciences as linear linkers, in order to arrange various functionalities in a defined spatial manner. In this Review article, recent developments and usages of these special, rectilinear systems are discussed. Furthermore, we focus on covalently linked, nonconjugated linear arrangements and discuss the physical and chemical properties and differences of individual linkers, as well as their application in material and medicinal sciences.
Subject(s)
Hydrocarbons, Cyclic/chemistry , Biochemistry , Catalysis , Chemistry, Pharmaceutical , Hydrocarbons, Cyclic/chemical synthesis , Materials Science , ThermodynamicsABSTRACT
A range of natural products from marine invertebrates, bacteria and fungi have been assessed as leads for nature-inspired antifouling (AF) biocides, but little attention has been paid to microalgal-derived compounds. This study assessed the AF activity of the spirocyclic imine portimine (1), which is produced by the benthic mat-forming dinoflagellate Vulcanodinium rugosum. Portimine displayed potent AF activity in a panel of four macrofouling bioassays (EC50 0.06-62.5 ng ml-1), and this activity was distinct from that of the related compounds gymnodimine-A (2), 13-desmethyl spirolide C (3), and pinnatoxin-F (4). The proposed mechanism of action for portimine is induction of apoptosis, based on the observation that portimine inhibited macrofouling organisms at developmental stages known to involve apoptotic processes. Semisynthetic modification of select portions of the portimine molecule was subsequently undertaken. Observed changes in bioactivity of the resulting semisynthetic analogues of portimine were consistent with portimine's unprecedented 5-membered imine ring structure playing a central role in its AF activity.
Subject(s)
Alkaloids/pharmacology , Biofouling/prevention & control , Heterocyclic Compounds, 3-Ring/pharmacology , Hydrocarbons, Cyclic/pharmacology , Imines/pharmacology , Microalgae/chemistry , Spiro Compounds/pharmacology , Alkaloids/chemical synthesis , Alkaloids/chemistry , Aquatic Organisms/drug effects , Heterocyclic Compounds, 3-Ring/chemical synthesis , Heterocyclic Compounds, 3-Ring/chemistry , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/chemistry , Imines/chemical synthesis , Imines/chemistry , Molecular Structure , Spiro Compounds/chemical synthesis , Spiro Compounds/chemistry , Structure-Activity RelationshipABSTRACT
A diastereodivergent hydroarylation of terminal alkynes is accomplished using tandem catalysis. The hydroarylation allows highly selective synthesis of both E and Z diastereoisomers of aryl alkenes, from the same set of starting materials, using the same combination of palladium and copper catalysts. The selectivity is controlled by simple changes in the stoichiometry of the alcohol additive. The hydroarylation has excellent substrate scope and can be accomplished in the presence of various classes of compounds, including esters, nitriles, alkyl halides, epoxides, carbamates, acetals, ethers, silyl ethers, and thioethers. The Z-selective hydroarylation is accomplished using a new approach based on tandem Sonogashira coupling and catalytic semireduction. The E-selective hydroarylation involves an additional catalytic isomerization of the Z-alkene. Our explorations of the reaction mechanism explain the role of individual reaction components and how the subtle changes in the reaction conditions influence the rates of specific steps of the hydroarylation. Our studies also show that, although the Z- and E-selective hydroarylation reactions are mechanistically closely related, the roles of the palladium and copper catalysts in the two reactions are different.
Subject(s)
Alkynes/chemical synthesis , Alkynes/chemistry , Catalysis , Copper/chemistry , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/chemistry , Molecular Structure , Palladium/chemistryABSTRACT
Chemical C-glycosylation has been well developed to improve stereoselectivity in recent years. Due to its high efficiency to build C-glycosides or O-cyclic compounds, C-glycosylation has found widespread use in the synthesis of biologically active molecules. This review highlights the C-glycosylation methods that have been practised in the total synthesis of natural products and pharmaceuticals in the past decade.
Subject(s)
Biological Products/chemical synthesis , Chemistry Techniques, Synthetic/methods , Pharmaceutical Preparations/chemical synthesis , Biological Products/chemistry , Glycosides/chemical synthesis , Glycosides/chemistry , Glycosylation , Hydrocarbons, Acyclic/chemical synthesis , Hydrocarbons, Acyclic/chemistry , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Cyclic/chemistry , Pharmaceutical Preparations/chemistry , StereoisomerismABSTRACT
Although the brominated flame retardant 1,2,5,6,9,10-hexabromocyclododecane (HBCD) has been widely used to reduce the flammability of polymeric materials, it is a toxic and persistent organic compound. In this paper, we report an efficient method for the debromination of HBCD by using a Pd-catalyzed system. HBCD was completely debrominated to the C12 cyclic compounds such as cyclododecatriene, cyclododecadiene, and cyclododecene in a solution of 2-propanol/methanol (99:1, v/v) containing dissolved NaOH in the presence of a silica-supported Pd catalyst (Pd/SiO2) at 35 °C. The reaction achieved product yields of 92% for the bromine-free products and 94% for the released Br ions. In the absence of Pd/SiO2, HBCD was partially debrominated to yield penta-, tetra-, and tribrominated C12 cyclic compounds. The HBCD debromination pathway seems to involve both HBr elimination by reaction with NaOH and Pd-catalyzed hydrodebromination by hydrogen transfer from 2-propanol.
Subject(s)
Environmental Restoration and Remediation/methods , Flame Retardants , Halogenation , Hydrocarbons, Brominated/chemistry , Hydrocarbons, Cyclic/chemical synthesis , 2-Propanol , Bromine , Catalysis , Hydrogen/chemistry , Methanol , Palladium/chemistry , Silicon DioxideABSTRACT
Novel Rh(I)-catalyzed cyclizations through a different type of rhodacycle intermediate which is formed by hydroacylation of 4,6-dienal or oxidative addition of diene and alkene are described. Hydroacylation of 4,6-dienal afforded various 7-membered rings in good to high yields, while cycloisomerization of diene and alkene provided 5- or 6-membered rings in good yields. On the basis of these studies, we have also succeeded in developing the sequential reaction of hydroacylation followed by cycloisomerization to produce bicyclic compounds in a stereoselective manner and thus this reaction was expanded to the synthesis of epiglobulol. Furthermore, both Rh(I)-catalyzed hydroacylation and cycloisomerization using ionic liquid (IL) as a solvent were investigated and it was found that the IL recovered after the reaction, which contains the Rh(I) catalyst, could be recycled several times without loss of catalytic activity.
Subject(s)
Alkenes/chemistry , Chemistry Techniques, Synthetic/methods , Hydrocarbons, Cyclic/chemical synthesis , Rhodium/chemistry , Acylation , Alkenes/chemical synthesis , Bridged Bicyclo Compounds/chemical synthesis , Bridged Bicyclo Compounds/chemistry , Cyclization , Hydrocarbons, Cyclic/chemistry , Isomerism , Oxidation-ReductionABSTRACT
A straightforward and convenient approach for trifluoromethylthiolation of various acyclic and cyclic ketones with PhNHSCF3 is described. The reaction proceeds smoothly in the presence of acetyl chloride at room temperature and affords α-trifluoromethylthiolated ketones in fair to good yields.
Subject(s)
Hydrocarbons, Cyclic/chemistry , Hydrocarbons, Fluorinated/chemistry , Ketones/chemistry , Sulfhydryl Compounds/chemistry , Halogenation , Hydrocarbons, Cyclic/chemical synthesis , Hydrocarbons, Fluorinated/chemical synthesis , Ketones/chemical synthesis , Methylation , Sulfhydryl Compounds/chemical synthesisABSTRACT
Switchable tandem intramolecular aza-Michael/Michael and double aza-Michael reactions allow the oriented synthesis of highly functionalised cyclic skeletons. Conjugate addition of deactivated anilines triggers chemo- and stereo-divergent ring-closure reaction pathways with a striking selectivity depending on reaction conditions.
Subject(s)
Acids/chemistry , Amines/chemistry , Aza Compounds/chemistry , Hydrocarbons, Cyclic/chemistry , Hydrocarbons, Cyclic/chemical synthesis , Catalysis , Molecular Structure , StereoisomerismABSTRACT
Sulfinic acids and their salts have recently emerged as versatile coupling partners to efficiently access a wide variety of hetero- and carbocyclic compounds, under relatively mild conditions. Their growing importance is attributable to their dual capacity for acting as nucleophilic or electrophilic reagents. This report summarizes recent advances in the preparation and use of sulfinates in organic synthesis.
Subject(s)
Heterocyclic Compounds/chemical synthesis , Hydrocarbons, Cyclic/chemical synthesis , Sulfinic Acids/chemistry , Heterocyclic Compounds/chemistry , Hydrocarbons, Cyclic/chemistry , Molecular StructureABSTRACT
A novel copper-catalyzed intermolecular trifluoromethylarylation of alkenes is developed using less active ether-type Togni's reagent under mild reaction conditions. Various alkenes and diverse arylboronic acids are compatible with these conditions. Preliminary mechanistic studies reveal that a mutual activation process between arylboronic acid and CF3(+) reagent is essential. In addition, the reaction might involve a rate-determining transmetalation, and the final aryl C-C bond is derived from reductive elimination of the aryl(alkyl)Cu(III) intermediate.