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1.
Respir Physiol Neurobiol ; 296: 103800, 2022 02.
Article in English | MEDLINE | ID: mdl-34626831

ABSTRACT

Infants born with neonatal opioid withdrawal syndrome (NOWS) can display abnormal cardiorespiratory patterns including tachypnea, tachycardia, and impaired ventilatory responses to hypoxia (HVR) and hypercapnia (HCVR). Chronic morphine exposure is associated with increased midbrain microglial expression. Using a rat model of pre- and post-natal morphine exposure, we assessed cardiorespiratory features of NOWS (resting tachycardia and tachypnea) including the attenuated HVR and HCVR and whether they are associated with increased brainstem microglia expression. Pregnant rats (dams) received twice-daily subcutaneous injections of morphine (5 mg/kg) during the third (last) week of pregnancy to simulate 3rd trimester in utero opioid exposure. Offspring then received once-daily subcutaneous injections of morphine (0.5 mg/kg) until postnatal (P) day P10 days of age to simulate postnatal morphine therapy. Cardiorespiratory responses were assessed 24 h later (P11 days) following spontaneous withdrawal. Compared to saline-treated pups, morphine-exposed offspring exhibited tachycardia and tachypnea as well as an attenuated HVR and HCVR. Microglial cell counts were increased in the nucleus tractus solitarius (nTS), dorsal motor nucleus of the vagus (DMNV) and nucleus ambiguous (NAamb), but not the retrapezoid nucleus (RTN) or the non-cardiorespriatory region, the cuneate nucleus (CN). These data suggest that the cardiorespiratory features and autonomic dysregulation in NOWS infants may be associated with altered microglial function in specific brainstem cardiorespiratory control regions.


Subject(s)
Brain Stem , Infant, Newborn, Diseases , Microglia , Opioid-Related Disorders , Substance Withdrawal Syndrome , Tachycardia , Tachypnea , Animals , Animals, Newborn , Brain Stem/immunology , Brain Stem/physiopathology , Disease Models, Animal , Female , Humans , Hypercapnia/immunology , Hypercapnia/physiopathology , Hypoxia/immunology , Hypoxia/physiopathology , Infant, Newborn , Infant, Newborn, Diseases/etiology , Infant, Newborn, Diseases/immunology , Infant, Newborn, Diseases/physiopathology , Microglia/immunology , Opioid-Related Disorders/complications , Opioid-Related Disorders/immunology , Opioid-Related Disorders/physiopathology , Pregnancy , Prenatal Exposure Delayed Effects/immunology , Prenatal Exposure Delayed Effects/physiopathology , Rats , Substance Withdrawal Syndrome/complications , Substance Withdrawal Syndrome/immunology , Substance Withdrawal Syndrome/physiopathology , Tachycardia/etiology , Tachycardia/immunology , Tachycardia/physiopathology , Tachypnea/etiology , Tachypnea/immunology , Tachypnea/physiopathology
2.
Med Hypotheses ; 144: 110002, 2020 Nov.
Article in English | MEDLINE | ID: mdl-32590322

ABSTRACT

Straying away from a sedentary lifestyle is essential, especially in these troubled times of a global pandemic to reverse the ill effects associated with the health risks as mentioned earlier. In the view of anticipated effects on immune system and prevention against influenza and Covid-19, globally moderate to vigorous exercises are advocated wearing protective equipment such as facemasks. Though WHO supports facemasks only for Covid-19 patients, healthy "social exercisers" too exercise strenuously with customized facemasks or N95 which hypothesized to pose more significant health risks and tax various physiological systems especially pulmonary, circulatory and immune systems. Exercising with facemasks may reduce available Oxygen and increase air trapping preventing substantial carbon dioxide exchange. The hypercapnic hypoxia may potentially increase acidic environment, cardiac overload, anaerobic metabolism and renal overload, which may substantially aggravate the underlying pathology of established chronic diseases. Further contrary to the earlier thought, no evidence exists to claim the facemasks during exercise offer additional protection from the droplet transfer of the virus. Hence, we recommend social distancing is better than facemasks during exercise and optimal utilization rather than exploitation of facemasks during exercise.


Subject(s)
COVID-19/prevention & control , Exercise , Hypercapnia/etiology , Hypoxia/etiology , Masks/adverse effects , Pulmonary Ventilation , Air Microbiology , Anaerobiosis , Brain/physiopathology , COVID-19/immunology , COVID-19/transmission , Carbon Dioxide/blood , Exercise/physiology , Guidelines as Topic , Heart/physiopathology , Humans , Hypercapnia/blood , Hypercapnia/immunology , Hypercapnia/physiopathology , Hypoxia/blood , Hypoxia/immunology , Hypoxia/physiopathology , Kidney/physiopathology , Muscle, Skeletal/physiopathology , Oxygen/blood , Oxygen Consumption , SARS-CoV-2/isolation & purification , World Health Organization
3.
J Immunol ; 205(2): 489-501, 2020 07 15.
Article in English | MEDLINE | ID: mdl-32540997

ABSTRACT

Hypercapnia (HC), elevation of the partial pressure of CO2 in blood and tissues, is a risk factor for mortality in patients with severe acute and chronic lung diseases. We previously showed that HC inhibits multiple macrophage and neutrophil antimicrobial functions and increases the mortality of bacterial pneumonia in mice. In this study, we show that normoxic HC increases viral replication, lung injury, and mortality in mice infected with influenza A virus (IAV). Elevated CO2 increased IAV replication and inhibited antiviral gene and protein expression in macrophages in vivo and in vitro. HC potentiated IAV-induced activation of Akt, whereas specific pharmacologic inhibition or short hairpin RNA knockdown of Akt1 in alveolar macrophages blocked HC's effects on IAV growth and the macrophage antiviral response. Our findings suggest that targeting Akt1 or the downstream pathways through which elevated CO2 signals could enhance macrophage antiviral host defense and improve clinical outcomes in hypercapnic patients with advanced lung disease.


Subject(s)
Hypercapnia/immunology , Influenza A virus/physiology , Influenza, Human/immunology , Lung/pathology , Macrophages/immunology , Oncogene Protein v-akt/metabolism , Orthomyxoviridae Infections/immunology , Animals , Cells, Cultured , Gene Expression Regulation , Humans , Immunity, Cellular , Immunosuppression Therapy , Lung/virology , Macrophage Activation , Mice , Mice, Inbred C57BL , Signal Transduction , Virus Replication
4.
Anesthesiology ; 128(1): 130-139, 2018 01.
Article in English | MEDLINE | ID: mdl-29023354

ABSTRACT

BACKGROUND: Hypercapnia alleviates pulmonary ischemia-reperfusion injury, regulates T lymphocytes, and inhibits immune reaction. This study aimed to evaluate the effect of hypercapnia on acute cellular rejection in a rat lung transplantation model. METHODS: Recipient rats in sham-operated (Wistar), isograft (Wistar to Wistar), and allograft (Sprague-Dawley to Wistar) groups were ventilated with 50% oxygen, whereas rats in the hypercapnia (Sprague-Dawley to Wistar) group were administered 50% oxygen and 8% carbon dioxide for 90 min during reperfusion (n = 8). Recipients were euthanized 7 days after transplantation. RESULTS: The hypercapnia group showed a higher oxygenation index (413 ± 78 vs. 223 ± 24), lower wet weight-to-dry weight ratio (4.23 ± 0.54 vs. 7.04 ± 0.80), lower rejection scores (2 ± 1 vs. 4 ± 1), and lower apoptosis index (31 ± 6 vs. 57 ± 4) as compared with the allograft group. The hypercapnia group showed lower CD8 (17 ± 4 vs. 31 ± 3) and CD68 (24 ± 3 vs. 43 ± 2), lower CD8 T cells (12 ± 2 vs. 35 ± 6), and higher CD4/CD8 ratio (2.2 ± 0.6 vs. 1.1 ± 0.4) compared to the allograft group. Tumor necrosis factor-α (208 ± 40 vs. 292 ± 49), interleukin-2 (30.6 ± 6.7 vs. 52.7 ± 8.3), and interferon-γ (28.1 ± 4.9 vs. 62.7 ± 10.1) levels in the hypercapnia group were lower than those in allograft group. CD4, CD4 T cells, and interleukin-10 levels were similar between groups. CONCLUSIONS: Hypercapnia ameliorated acute cellular rejection in a rat lung transplantation model.


Subject(s)
Graft Rejection/metabolism , Hypercapnia/metabolism , Lung Transplantation/adverse effects , Lung/metabolism , T-Lymphocytes/metabolism , Allografts/immunology , Allografts/metabolism , Allografts/pathology , Animals , Graft Rejection/immunology , Graft Rejection/pathology , Hypercapnia/immunology , Hypercapnia/pathology , Lung/immunology , Lung/pathology , Lung Transplantation/trends , Male , Random Allocation , Rats , Rats, Sprague-Dawley , Rats, Wistar , Species Specificity , T-Lymphocytes/immunology
5.
J Biol Chem ; 292(27): 11561-11571, 2017 07 07.
Article in English | MEDLINE | ID: mdl-28507099

ABSTRACT

CO2 is a physiological gas normally produced in the body during aerobic respiration. Hypercapnia (elevated blood pCO2 >≈50 mm Hg) is a feature of several lung pathologies, e.g. chronic obstructive pulmonary disease. Hypercapnia is associated with increased susceptibility to bacterial infections and suppression of inflammatory signaling. The NF-κB pathway has been implicated in these effects; however, the molecular mechanisms underpinning cellular sensitivity of the NF-κB pathway to CO2 are not fully elucidated. Here, we identify several novel CO2-dependent changes in the NF-κB pathway. NF-κB family members p100 and RelB translocate to the nucleus in response to CO2 A cohort of RelB protein-protein interactions (e.g. with Raf-1 and IκBα) are altered by CO2 exposure, although others are maintained (e.g. with p100). RelB is processed by CO2 in a manner dependent on a key C-terminal domain located in its transactivation domain. Loss of the RelB transactivation domain alters NF-κB-dependent transcriptional activity, and loss of p100 alters sensitivity of RelB to CO2 Thus, we provide molecular insight into the CO2 sensitivity of the NF-κB pathway and implicate altered RelB/p100-dependent signaling in the CO2-dependent regulation of inflammatory signaling.


Subject(s)
Carbon Dioxide/immunology , Hypercapnia/immunology , NF-kappa B p52 Subunit/immunology , Signal Transduction/immunology , Transcription Factor RelB/immunology , A549 Cells , Animals , Humans , Hypercapnia/genetics , Hypercapnia/pathology , Mice , NF-kappa B p52 Subunit/genetics , Protein Domains , Signal Transduction/genetics , Transcription Factor RelB/genetics , Transcription, Genetic/genetics , Transcription, Genetic/immunology
6.
Respir Physiol Neurobiol ; 242: 45-51, 2017 08.
Article in English | MEDLINE | ID: mdl-28330778

ABSTRACT

Perinatal inflammation and infection are commonly associated with various respiratory morbidities in preterm infants including apnea of prematurity. In this study, we investigated whether pulmonary inflammation via intra-tracheal micro-injection of lipopolysaccharide (LPS) into neonatal rats modifies respiratory neural control via an IL-1ß receptor-dependent mechanism. Prior to an intra-tracheal micro-injection of LPS (1mg/kg), 10day old (Postnatal age, P10) rats received an intraperitoneal (i.p.) or intracisternal (i.c.) micro-injection of the IL-1ß receptor antagonist AF12198. Whole-body plethysmography was performed two hours later to assess the magnitude of the acute hypoxic (HVR) and hypercapnic (HCVR) ventilatory responses. Intra-tracheal LPS dose-dependently attenuated the acute HVR compared to saline (control) treated rats, whereas the HCVR was not affected. Pre-treatment with an i.c. (but not i.p.) micro-injection of AF12198 15min prior to LPS prevented the attenuated HVR. These data indicate that intrapulmonary inflammation affects brainstem respiratory neural pathways mediating the ventilatory response to acute hypoxia via an IL-1ß-dependent pathway. These findings are relevant to our understanding of the way that pulmonary inflammation may affect central neural mechanisms of respiratory insufficiency commonly seen in preterm infants.


Subject(s)
Brain Stem/immunology , Hypoxia/immunology , Interleukin-1beta/metabolism , Pneumonia/immunology , Respiration , Animals , Animals, Newborn , Brain Stem/drug effects , Central Nervous System Agents/pharmacology , Escherichia coli , Hypercapnia/immunology , Injections, Intraperitoneal , Lipopolysaccharides , Male , Microinjections , Neural Pathways/drug effects , Neural Pathways/immunology , Plethysmography , Proteins/pharmacology , Random Allocation , Rats, Sprague-Dawley , Receptors, Interleukin-1/antagonists & inhibitors , Receptors, Interleukin-1/metabolism , Trachea
7.
J Immunol ; 196(2): 655-667, 2016 Jan 15.
Article in English | MEDLINE | ID: mdl-26643480

ABSTRACT

Hypercapnia, elevated partial pressure of CO2 in blood and tissue, develops in many patients with chronic severe obstructive pulmonary disease and other advanced lung disorders. Patients with advanced disease frequently develop bacterial lung infections, and hypercapnia is a risk factor for mortality in such individuals. We previously demonstrated that hypercapnia suppresses induction of NF-κB-regulated innate immune response genes required for host defense in human, mouse, and Drosophila cells, and it increases mortality from bacterial infections in both mice and Drosophila. However, the molecular mediators of hypercapnic immune suppression are undefined. In this study, we report a genome-wide RNA interference screen in Drosophila S2* cells stimulated with bacterial peptidoglycan. The screen identified 16 genes with human orthologs whose knockdown reduced hypercapnic suppression of the gene encoding the antimicrobial peptide Diptericin (Dipt), but did not increase Dipt mRNA levels in air. In vivo tests of one of the strongest screen hits, zinc finger homeodomain 2 (Zfh2; mammalian orthologs ZFHX3/ATBF1 and ZFHX4), demonstrate that reducing zfh2 function using a mutation or RNA interference improves survival of flies exposed to elevated CO2 and infected with Staphylococcus aureus. Tissue-specific knockdown of zfh2 in the fat body, the major immune and metabolic organ of the fly, mitigates hypercapnia-induced reductions in Dipt and other antimicrobial peptides and improves resistance of CO2-exposed flies to infection. Zfh2 mutations also partially rescue hypercapnia-induced delays in egg hatching, suggesting that Zfh2's role in mediating responses to hypercapnia extends beyond the immune system. Taken together, to our knowledge, these results identify Zfh2 as the first in vivo mediator of hypercapnic immune suppression.


Subject(s)
DNA-Binding Proteins/immunology , Drosophila Proteins/immunology , Hypercapnia/immunology , Staphylococcal Infections/complications , Animals , Blotting, Western , Disease Models, Animal , Drosophila , Gene Knockdown Techniques , Hypercapnia/microbiology , Immunity, Innate/immunology , RNA Interference , Staphylococcal Infections/immunology , Staphylococcus aureus
8.
Int Immunopharmacol ; 27(1): 76-84, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25921030

ABSTRACT

Hypercapnia is known to have immunoregulatory effects within the lung. Cell culture systems demonstrate this in both macrophages and alveolar cell lines, suggesting that the alveoli are affected by changes in CO2 levels. We hypothesized that hypercapnia would also modulate human bronchial epithelial cell immune responses. Innate immune responses to Pam3CSK4 (TLR2 ligand), LPS (TLR4 ligand) and a complex innate immune stimulus, an extract from the organic dust of swine confinement barns (barn dust extract or BDE), were tested in a human bronchial epithelial cell line, BEAS-2B. Both TLR ligands showed a decrease in IL-6 and IL-8 production, and an increase in MCP-1 in response to elevated CO2 indicating an enhancement in cytokine production to hypercapnia. This change was not reflected in expression levels of TLR receptor RNA which remained unchanged in response to elevated CO2. Interestingly, barn dust showed an increase in IL-6, IL-8 and MCP-1 response at 9% CO2, suggesting that elevated CO2 exerts different effects on different stimuli. Our results show that airway epithelial cell immune responses to barn dust respond differently to hypercapnic conditions than individual TLR ligands.


Subject(s)
Bronchi/pathology , Carbon Dioxide/blood , Hypercapnia/immunology , Respiratory Mucosa/metabolism , Air Pollution, Indoor/adverse effects , Animals , Cell Line , Chemokine CCL2/metabolism , Dust/immunology , Immunity, Innate , Inflammation Mediators/metabolism , Interleukin-8/metabolism , Lipopeptides/pharmacology , Lipopolysaccharides/pharmacology , Respiratory Mucosa/drug effects , Respiratory Mucosa/pathology , Swine , Toll-Like Receptor 2/agonists , Toll-Like Receptor 4/agonists
9.
J Immunol ; 194(11): 5388-96, 2015 Jun 01.
Article in English | MEDLINE | ID: mdl-25895534

ABSTRACT

Hypercapnia, the elevation of CO2 in blood and tissue, commonly develops in patients with advanced lung disease and severe pulmonary infections, and it is associated with high mortality. We previously reported that hypercapnia alters expression of host defense genes, inhibits phagocytosis, and increases the mortality of Pseudomonas pneumonia in mice. However, the effect of hypercapnia on autophagy, a conserved process by which cells sequester and degrade proteins and damaged organelles that also plays a key role in antimicrobial host defense and pathogen clearance, has not previously been examined. In the present study we show that hypercapnia inhibits autophagy induced by starvation, rapamycin, LPS, heat-killed bacteria, and live bacteria in the human macrophage. Inhibition of autophagy by elevated CO2 was not attributable to acidosis. Hypercapnia also reduced macrophage killing of Pseudomonas aeruginosa. Moreover, elevated CO2 induced the expression of Bcl-2 and Bcl-xL, antiapoptotic factors that negatively regulate autophagy by blocking Beclin 1, an essential component of the autophagy initiation complex. Furthermore, small interfering RNA targeting Bcl-2 and Bcl-xL and the small molecule Z36, which blocks Bcl-2 and Bcl-xL binding to Beclin 1, prevented hypercapnic inhibition of autophagy and bacterial killing. These results suggest that targeting the Bcl-2/Bcl-xL-Beclin 1 interaction may hold promise for ameliorating hypercapnia-induced immunosuppression and improving resistance to infection in patients with advanced lung disease and hypercapnia.


Subject(s)
Autophagy/immunology , Hypercapnia/immunology , Macrophages, Alveolar/immunology , Proto-Oncogene Proteins c-bcl-2/genetics , bcl-X Protein/genetics , Acidosis , Animals , Apoptosis Regulatory Proteins/antagonists & inhibitors , Autophagy/drug effects , Beclin-1 , Carbon Dioxide/blood , Carbon Dioxide/pharmacology , Cell Line , Humans , Hypercapnia/blood , Indoles/pharmacology , Lipopolysaccharides , Lung Diseases/pathology , Macrophages, Alveolar/microbiology , Membrane Proteins/antagonists & inhibitors , Mice , Phagocytosis/drug effects , Protein Binding/drug effects , Proto-Oncogene Proteins c-bcl-2/biosynthesis , Pseudomonas aeruginosa/immunology , RNA Interference , RNA, Small Interfering , Sirolimus/pharmacology , bcl-X Protein/biosynthesis
10.
Exp Biol Med (Maywood) ; 239(12): 1597-605, 2014 Dec.
Article in English | MEDLINE | ID: mdl-25013165

ABSTRACT

T cells play a key role in lung ischemia/reperfusion injury (IRI). Hypercapnia has been indicated to decrease IRI and inhibit immunity. This study aimed to evaluate the effects of hypercapnia on T cells during lung IRI and to identify the underlying mechanism of these effects. In the in vivo study, rat recipients of lung transplants were randomized into a control group M and a hypercapnia group H. Peripheral blood T cells and cytokines were analyzed during reperfusion. In the in vitro study, we analyzed the T cells and cytokine levels in culture media from phytohemagglutinin-stimulated T cells from normal rats, stimulated under the normal (group C), hypercapnic (group H), or buffer hypercapnic (group BH) condition. In the in vivo study, the CD3(+)/CD4(+) T-cell ratio and interleukin (IL)-2, IL-8, interferon (IFN)-γ, intracellular adhesion molecule (ICAM)-1, and P-selectin levels were decreased, but the IL-4 and IL-10 levels were increased, after reperfusion in group H compared to group M. In the in vitro study, groups H and BH exhibited a decreased CD2(+)/CD28(+) ratio and IL-2 and IFN-γ levels, but elevated IL-4 and IL-10 levels, compared to group C. The CD2(+)/CD28(+) ratio was not different between groups BH and H; however, group H evidenced a lower IL-2 level and higher IL-4 and IL-10 levels compared to group BH. Hypercapnia decreased the CD3(+)/CD4(+) T-cell ratio and pro-inflammatory cytokine levels, but promoted anti-inflammatory factors in lung IRI. Hypercapnia inhibits CD2 and CD28 in T cells by CO2 and modulates T-cell cytokines via acidosis.


Subject(s)
Hypercapnia/immunology , Reperfusion Injury , T-Lymphocytes/physiology , Animals , Antigens, CD/analysis , Cells, Cultured , Cytokines/metabolism , Lung Transplantation , Random Allocation , Rats, Wistar
11.
Am J Respir Cell Mol Biol ; 49(5): 821-8, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23777386

ABSTRACT

Hypercapnia, an elevation of the level of carbon dioxide (CO2) in blood and tissues, is a marker of poor prognosis in chronic obstructive pulmonary disease and other pulmonary disorders. We previously reported that hypercapnia inhibits the expression of TNF and IL-6 and phagocytosis in macrophages in vitro. In the present study, we determined the effects of normoxic hypercapnia (10% CO2, 21% O2, and 69% N2) on outcomes of Pseudomonas aeruginosa pneumonia in BALB/c mice and on pulmonary neutrophil function. We found that the mortality of P. aeruginosa pneumonia was increased in 10% CO2-exposed compared with air-exposed mice. Hypercapnia increased pneumonia mortality similarly in mice with acute and chronic respiratory acidosis, indicating an effect unrelated to the degree of acidosis. Exposure to 10% CO2 increased the burden of P. aeruginosa in the lungs, spleen, and liver, but did not alter lung injury attributable to pneumonia. Hypercapnia did not reduce pulmonary neutrophil recruitment during infection, but alveolar neutrophils from 10% CO2-exposed mice phagocytosed fewer bacteria and produced less H2O2 than neutrophils from air-exposed mice. Secretion of IL-6 and TNF in the lungs of 10% CO2-exposed mice was decreased 7 hours, but not 15 hours, after the onset of pneumonia, indicating that hypercapnia inhibited the early cytokine response to infection. The increase in pneumonia mortality caused by elevated CO2 was reversible when hypercapnic mice were returned to breathing air before or immediately after infection. These results suggest that hypercapnia may increase the susceptibility to and/or worsen the outcome of lung infections in patients with severe lung disease.


Subject(s)
Hypercapnia/complications , Lung/immunology , Neutrophils/immunology , Pneumonia, Bacterial/complications , Pseudomonas aeruginosa/pathogenicity , Acidosis, Respiratory/immunology , Acidosis, Respiratory/microbiology , Animals , Bacterial Load , Disease Models, Animal , Female , HL-60 Cells , Humans , Hypercapnia/immunology , Hypercapnia/pathology , Inflammation Mediators/metabolism , Interleukin-6/metabolism , Lung/microbiology , Lung/pathology , Mice , Mice, Inbred BALB C , Neutrophils/microbiology , Phagocytosis , Pneumonia, Bacterial/immunology , Pneumonia, Bacterial/microbiology , Pneumonia, Bacterial/pathology , Reactive Oxygen Species/metabolism , Time Factors , Tumor Necrosis Factor-alpha/metabolism
12.
Artif Organs ; 37(12): 1049-58, 2013 Dec.
Article in English | MEDLINE | ID: mdl-23738674

ABSTRACT

Experimental data indicate that hypercapnic adidosis has anti-inflammatory effects. These anti-inflammatory effects may even be a beneficial property in case of low tidal volume ventilation with consecutive hypercapnic acidosis. It is unclear whether these anti-inflammatory effects predominate in critically ill patients who suffer from multiple pro- and anti-inflammatory insults like extracorporeal organ support (pro-inflammatory), metabolic acidosis (pro- and anti-inflammatory), as well as hypoxia (pro-inflammatory). Eighteen pigs were randomized into three groups, mechanically ventilated and connected to a continuous veno-venous hemofiltration (CVVH) as pro-inflammatory insult. A reference group with normal acid-base state obtained normoventilation; a normoxemic acidemia group obtained normoxemic, mixed acidemia due to infusion of lactic and hyperchloremic acid and low tidal volume ventilation, and in a hypoxemic acidemia group the mixed acidemia was paralleled by hypoxemia. Lung histology including pulmonary leukocyte invasion, blood gases, blood cell counts, and hemodynamics were examined. The histological examination of the lungs of acidemic pigs showed a suppressed invasion of leukocytes and thinner alveolar walls compared with normoventilated and with hypoxemic pigs. Enhanced congestion and alveolar red blood cells (RBCs) combined with an increase of the pulmonary artery pressure were observed in acidemic pigs in comparison with the reference group. Normoxemic acidemia reduced the pro-inflammatory reaction to the CVVH and mechanical ventilation in the ventilated lung areas in the form of pulmonary leukocyte invasion. However, this did not result in reduced scores for lung injury. Instead, an increased score for criteria which represent lung injury (congestion and alveolar RBCs) was observed in acidemic pigs.


Subject(s)
Acidosis/complications , Hemofiltration/adverse effects , Hypercapnia/complications , Lung , Respiration, Artificial/adverse effects , Ventilator-Induced Lung Injury/etiology , Acidosis/immunology , Acidosis/physiopathology , Animals , Hemodynamics , Hypercapnia/immunology , Hypercapnia/physiopathology , Inflammation Mediators/metabolism , Leukocytes/immunology , Lung/immunology , Lung/pathology , Lung/physiopathology , Male , Risk Factors , Swine , Time Factors , Ventilator-Induced Lung Injury/diagnosis , Ventilator-Induced Lung Injury/immunology , Ventilator-Induced Lung Injury/physiopathology , Ventilator-Induced Lung Injury/prevention & control
13.
Fiziol Cheloveka ; 38(2): 119-29, 2012.
Article in Russian | MEDLINE | ID: mdl-22679806

ABSTRACT

This review summarizes and analyzes the results of the present experimental studies indicating immune system involvement in control of breathing. The hypothesis about the role of cytokines in the mechanisms of respiratory muscle fatigue and reduced ventilatory sensitivity to hypercapnia during respiration with the added resistive loading is justified. The possible ways of implementing of respiratory cytokine effects are discussed.


Subject(s)
Airway Resistance , Cytokines/metabolism , Hypercapnia/physiopathology , Respiration/immunology , Respiratory Muscles/physiopathology , Cytokines/physiology , Diaphragm/cytology , Diaphragm/pathology , Humans , Hypercapnia/immunology , Monocytes/immunology , Muscle Cells/cytology , Muscle Cells/metabolism , Oxidative Stress , Respiratory Muscles/immunology
15.
FASEB J ; 24(7): 2178-90, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20181940

ABSTRACT

Elevated blood and tissue CO(2), or hypercapnia, is common in severe lung disease. Patients with hypercapnia often develop lung infections and have an increased risk of death following pneumonia. To explore whether hypercapnia interferes with host defense, we studied the effects of elevated P(CO2) on macrophage innate immune responses. In differentiated human THP-1 macrophages and human and mouse alveolar macrophages stimulated with lipopolysaccharide (LPS) and other Toll-like receptor ligands, hypercapnia inhibited expression of tumor necrosis factor and interleukin (IL)-6, nuclear factor (NF)-kappaB-dependent cytokines critical for antimicrobial host defense. Inhibition of IL-6 expression by hypercapnia was concentration dependent, rapid, reversible, and independent of extracellular and intracellular acidosis. In contrast, hypercapnia did not down-regulate IL-10 or interferon-beta, which do not require NF-kappaB. Notably, hypercapnia did not affect LPS-induced degradation of IkappaB alpha, nuclear translocation of RelA/p65, or activation of mitogen-activated protein kinases, but it did block IL-6 promoter-driven luciferase activity in mouse RAW 264.7 macrophages. Elevated P(CO2) also decreased phagocytosis of opsonized polystyrene beads and heat-killed bacteria in THP-1 and human alveolar macrophages. By interfering with essential innate immune functions in the macrophage, hypercapnia may cause a previously unrecognized defect in resistance to pulmonary infection in patients with advanced lung disease.


Subject(s)
Carbon Dioxide/pharmacology , Hypercapnia/immunology , Interleukin-6/antagonists & inhibitors , Macrophages, Alveolar/immunology , Phagocytosis/drug effects , Tumor Necrosis Factor-alpha/antagonists & inhibitors , Animals , Cell Line, Tumor , Cytokines/biosynthesis , Humans , Immunity, Innate/drug effects , Interleukin-6/biosynthesis , Macrophages, Alveolar/drug effects , Mice , Tumor Necrosis Factor-alpha/biosynthesis
16.
Anesthesiology ; 112(2): 462-72, 2010 Feb.
Article in English | MEDLINE | ID: mdl-20068449

ABSTRACT

Acute respiratory distress syndrome is a devastating disease that causes substantial morbidity and mortality. Mechanical ventilation can worsen lung injury, whereas ventilatory strategies that reduce lung stretch, resulting in a "permissive" hypercapnic acidosis (HCA), improve outcome. HCA directly reduces nonsepsis-induced lung injury in preclinical models and, therefore, has therapeutic potential in these patients. These beneficial effects are mediated via inhibition of the host immune response, particularly cytokine signaling, phagocyte function, and the adaptive immune response. Of concern, these immunosuppressive effects of HCA may hinder the host response to microbial infection. Recent studies suggest that HCA is protective in the earlier phases of bacterial pneumonia-induced sepsis but may worsen injury in the setting of prolonged lung sepsis. In contrast, HCA is protective in preclinical models of early and prolonged systemic sepsis. Buffering of the HCA is not beneficial and may worsen pneumonia-induced injury.


Subject(s)
Acidosis/etiology , Hypercapnia/etiology , Sepsis/complications , Acidosis/immunology , Acidosis/microbiology , Critical Illness , Humans , Hypercapnia/immunology , Hypercapnia/microbiology , Lung Diseases/microbiology , Lung Diseases/pathology , Pneumonia/microbiology , Pneumonia/pathology , Sepsis/immunology , Sepsis/microbiology
17.
Proc Natl Acad Sci U S A ; 106(44): 18710-5, 2009 Nov 03.
Article in English | MEDLINE | ID: mdl-19846771

ABSTRACT

Elevated CO(2) levels (hypercapnia) frequently occur in patients with obstructive pulmonary diseases and are associated with increased mortality. However, the effects of hypercapnia on non-neuronal tissues and the mechanisms that mediate these effects are largely unknown. Here, we develop Drosophila as a genetically tractable model for defining non-neuronal CO(2) responses and response pathways. We show that hypercapnia significantly impairs embryonic morphogenesis, egg laying, and egg hatching even in mutants lacking the Gr63a neuronal CO(2) sensor. Consistent with previous reports that hypercapnic acidosis can suppress mammalian NF-kappaB-regulated innate immune genes, we find that in adult flies and the phagocytic immune-responsive S2* cell line, hypercapnia suppresses induction of specific antimicrobial peptides that are regulated by Relish, a conserved Rel/NF-kappaB family member. Correspondingly, modest hypercapnia (7-13%) increases mortality of flies inoculated with E. faecalis, A. tumefaciens, or S. aureus. During E. faecalis and A. tumefaciens infection, increased bacterial loads were observed, indicating that hypercapnia can decrease host resistance. Hypercapnic immune suppression is not mediated by acidosis, the olfactory CO(2) receptor Gr63a, or by nitric oxide signaling. Further, hypercapnia does not induce responses characteristic of hypoxia, oxidative stress, or heat shock. Finally, proteolysis of the Relish IkappaB-like domain is unaffected by hypercapnia, indicating that immunosuppression acts downstream of, or in parallel to, Relish proteolytic activation. Our results suggest that hypercapnic immune suppression is mediated by a conserved response pathway, and illustrate a mechanism by which hypercapnia could contribute to worse outcomes of patients with advanced lung disease, who frequently suffer from both hypercapnia and respiratory infections.


Subject(s)
Bacterial Infections/immunology , Carbon Dioxide/pharmacology , Drosophila melanogaster/immunology , Drosophila melanogaster/microbiology , Immunity, Innate/drug effects , Acidosis/complications , Acidosis/immunology , Animals , Antimicrobial Cationic Peptides/genetics , Antimicrobial Cationic Peptides/metabolism , Bacterial Infections/complications , Drosophila Proteins/metabolism , Drosophila melanogaster/drug effects , Drosophila melanogaster/growth & development , Gene Expression Regulation/drug effects , Hydrogen-Ion Concentration/drug effects , Hypercapnia/complications , Hypercapnia/immunology , Immune Tolerance/drug effects , Neurons/drug effects , Neurons/immunology , Nitric Oxide/metabolism , Protein Processing, Post-Translational/drug effects , Survival Analysis , Transcription Factors/metabolism
18.
Sleep ; 30(6): 723-7, 2007 Jun.
Article in English | MEDLINE | ID: mdl-17580593

ABSTRACT

STUDY OBJECTIVES: Obstructive sleep apnea (OSA) is characterized by repeated episodes of upper-airway obstruction during sleep leading to significant hypercapnic hypoxic conditions. These conditions are associated with increased levels of proinflammatory cytokines (including interleukin [IL]-6, tumor necrosis factor [TNF]-alpha, and C-reactive protein [CRP]) and subsequent increased cardiovascular risk. It is unclear whether hypercapnic hypoxia itself causes inflammatory perturbations. DESIGN: We evaluated circulating IL-6, TNF- a and CRP in a piglet model of infant OSA, following exposure to acute intermittent hypercapnic hypoxia (IHH). Study groups comprised of treatment (n = 8) and control (n = 8) groups. Treatment was two 90-minute sessions of IHH with arterial blood sampled before and after each IHH session. MEASUREMENTS AND RESULTS: IL-6, TNF-alpha and CRP levels were measured before and after IHH treatment sessions. Results showed an increase in IL-6 following the first session of IHH that was neither sustained, nor repeated, during a subsequent exposure. Using mixed-modelling, TNF-alpha changed between time points and groups. There were no changes in CRP over the duration of the study. CONCLUSION: These results suggest that acute hypoxia causes a transient increase in IL-6 levels and has implications for the pathogenesis of increased cardiovascular disease in OSA, especially in childhood.


Subject(s)
C-Reactive Protein/immunology , Hypercapnia , Hypoxia , Interleukin-6/immunology , Tumor Necrosis Factor-alpha/immunology , Acute Disease , Animals , C-Reactive Protein/metabolism , Hypercapnia/immunology , Hypercapnia/metabolism , Hypercapnia/therapy , Hypoxia/immunology , Hypoxia/metabolism , Hypoxia/therapy , Interleukin-6/blood , Male , Swine , Tumor Necrosis Factor-alpha/blood
19.
J Appl Physiol (1985) ; 99(5): 1782-8, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16024517

ABSTRACT

Patients with chronic bronchial asthma show a depressed ventilatory response to hypoxia (DVH), but the underlying mechanism remains unclear. We tested whether DVH existed in ovalbumin (Ova)-treated guinea pigs, an established animal model of asthma. Twelve guinea pigs were exposed to Ova (1% in saline) or saline aerosol (control) for 5 min, 5 days/wk, for 2 wk. After completing aerosol exposure, the animals were anesthetized and exposed to systemic hypoxia. Ova treatment had no effects on animal body weight, baseline cardiorespiratory variables, or arterial blood O2 and CO2 tensions, but it attenuated the ventilatory response to hypoxia (10 breaths of pure N2) by 65% (P < 0.05). When the animals were subjected to intracarotid injections of sodium cyanide (20 microg) and doxapram (2 mg) to selectively stimulate carotid chemoreceptors, the ventilatory responses were reduced by 50% (P < 0.05) and 74% (P < 0.05), respectively. In contrast, Ova exposure failed to affect the ventilatory response to CO2 (7% CO2-21% O2-balance N2 for 5 min; P > 0.05). Furthermore, the apneic response evoked by stimulating bronchopulmonary C fibers (PCFs) with right atrial injection of capsaicin (5 microg) was markedly increased in the Ova-sensitized group (5.02 +/- 1.56 s), compared with the control group (1.82 +/- 0.45 s; P < 0.05). These results suggest that Ova sensitization induces a DVH in guinea pigs, which probably results from an attenuation of the carotid chemoreceptor-mediated ventilatory excitation and an enhancement of the PCF-mediated ventilatory inhibition.


Subject(s)
Asthma/physiopathology , Hypoxia/physiopathology , Ovalbumin/pharmacology , Respiratory Mechanics/physiology , Animals , Asthma/immunology , Capsaicin/pharmacology , Carbon Dioxide/blood , Carotid Body/drug effects , Carotid Body/physiology , Disease Models, Animal , Doxapram/pharmacology , Enzyme Inhibitors/pharmacology , Guinea Pigs , Hypercapnia/immunology , Hypercapnia/physiopathology , Hypoxia/immunology , Male , Nerve Fibers, Unmyelinated/drug effects , Nerve Fibers, Unmyelinated/physiology , Nitrogen/pharmacology , Ovalbumin/immunology , Oxygen/blood , Respiratory System Agents/pharmacology , Sodium Cyanide/pharmacology
20.
J Surg Res ; 108(1): 32-8, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12443712

ABSTRACT

BACKGROUND: Experimental evidence supporting the safety of laparoscopic intervention during sepsis is limited. The purpose of this study was to evaluate the effects of pneumoperitoneum on immunologic and hemodynamic responses to peritoneal sepsis. MATERIALS AND METHODS: A porcine model of peritonitis was created using an intraperitoneal autologous fecal inoculum. Pigs were then subjected to one of four procedures 24 h postinoculation (n = 6 per group): laparotomy, CO(2) laparoscopy, helium laparoscopy, and anesthesia only (1.5% isoflurane in 100% O(2), mechanical ventilation). Venous blood samples were obtained prior to inoculation, and at 24 (prior to procedure), 30, 48, 72, and 96 h postinoculation to determine white blood count (WBC) with differential, C-reactive protein (CRP), tumor necrosis factor, and bacteremia. Heart rate, end-tidal CO(2) (ETCO(2)), mean arterial blood pressure (MAP), and arterial blood gas variables were also measured at baseline and every 30 min throughout the procedure. RESULTS: Postoperative blood cultures confirmed systemic bacteremia in all groups at all time periods postinoculation. Following inoculation, WBC, band cell count, and CRP remained elevated above baseline in all groups throughout the study (P < 0.01). However, no significant differences in these parameters were observed among groups. In the CO(2) laparoscopy group, MAP, ETCO(2), and arterial pCO(2) were increased above baseline, while pH was decreased throughout the procedure (P < 0.01). CONCLUSIONS: In this animal model of peritonitis, CO(2) pneumoperitoneum induced hypercapnia, acidemia, and systemic hypertension intraoperatively, without a discernable effect on systemic immune function.


Subject(s)
Peritonitis/immunology , Peritonitis/physiopathology , Pneumoperitoneum/immunology , Pneumoperitoneum/physiopathology , Acidosis/immunology , Acidosis/physiopathology , Animals , Blood Cell Count , C-Reactive Protein/metabolism , Carbon Dioxide/blood , Disease Models, Animal , Escherichia coli Infections/complications , Female , Heart Rate , Hypercapnia/immunology , Hypercapnia/physiopathology , Peritonitis/microbiology , Pneumoperitoneum/microbiology , Sepsis/immunology , Sepsis/microbiology , Sepsis/physiopathology , Staphylococcal Infections/complications , Streptococcal Infections/complications , Streptococcus bovis , Swine
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