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1.
Comput Math Methods Med ; 2022: 8332737, 2022.
Article in English | MEDLINE | ID: mdl-35281947

ABSTRACT

The goal of this study is to see how cold plasma affects rabbit bone tissue infected with osteoporosis. The search is divided into three categories: control, infected, and treated. The rabbits were subjected to cold plasma for five minutes in a room with a microwave plasma voltage of "175 V" and a gas flow of "2." A histopathological photograph of infected bone cells is obtained to demonstrate the influence of plasma on infected bone cells, as well as the extent of destruction and effect of plasma therapy before and after exposure. The findings of the search show that plasma has a clear impact on Ca and vitamin D levels. In the cold plasma, the levels of osteocalcin and alkali phosphates (ALP) respond as well. Image processing techniques (second-order gray level matrix) with textural elements are employed as an extra proof. The outcome gives good treatment indicators, and the image processing result corresponds to the biological result.


Subject(s)
Osteoporosis/therapy , Plasma Gases/therapeutic use , Animals , Bone and Bones/diagnostic imaging , Bone and Bones/metabolism , Calcium/metabolism , Computational Biology , Disease Models, Animal , Female , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Osteoporosis/diagnostic imaging , Osteoporosis/physiopathology , Phosphorus/blood , Rabbits , Vitamin D/metabolism
2.
Comput Math Methods Med ; 2022: 1991138, 2022.
Article in English | MEDLINE | ID: mdl-35295201

ABSTRACT

With the continuous development of science and technology, people can apply more and more technology to the cultivation of children's abilities. In the process of cultivating children's ability, the most fancy is the study of executive function, and this is the research topic of this article. In the past, training methods such as music, mindfulness, and exercise have been used in the study of children's executive abilities to promote the development of preschool children's executive functions. While various approaches have had some effect, researchers have been exploring more comprehensive approaches to effective training. This article is aimed at studying how to use image recognition technology to conduct an intervention analysis of breakdancing in promoting the executive function of preschool children. For this reason, this paper proposes image recognition technology based on deep learning neural network and conducts research, analysis, and improvement on related technologies obtained from deep learning. This makes it more suitable for the research topic of this article and design-related experiments and analysis to explore its related performance. The experimental results in this paper show that the improved image recognition technology has improved accuracy by 31.2%. And the performance of its algorithm is also improved by 21%, which can be very effective in monitoring preschool children during breakdancing.


Subject(s)
Child Development/physiology , Dancing/physiology , Dancing/psychology , Executive Function/physiology , Algorithms , Child, Preschool , Computational Biology , Deep Learning , Early Intervention, Educational/methods , Early Intervention, Educational/statistics & numerical data , Female , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Male , Movement/physiology , Neural Networks, Computer
3.
PLoS One ; 17(2): e0262286, 2022.
Article in English | MEDLINE | ID: mdl-35192638

ABSTRACT

Computer vision (CV) is widely used in the investigation of facial expressions. Applications range from psychological evaluation to neurology, to name just two examples. CV for identifying facial expressions may suffer from several shortcomings: CV provides indirect information about muscle activation, it is insensitive to activations that do not involve visible deformations, such as jaw clenching. Moreover, it relies on high-resolution and unobstructed visuals. High density surface electromyography (sEMG) recordings with soft electrode array is an alternative approach which provides direct information about muscle activation, even from freely behaving humans. In this investigation, we compare CV and sEMG analysis of facial muscle activation. We used independent component analysis (ICA) and multiple linear regression (MLR) to quantify the similarity and disparity between the two approaches for posed muscle activations. The comparison reveals similarity in event detection, but discrepancies and inconsistencies in source identification. Specifically, the correspondence between sEMG and action unit (AU)-based analyses, the most widely used basis of CV muscle activation prediction, appears to vary between participants and sessions. We also show a comparison between AU and sEMG data of spontaneous smiles, highlighting the differences between the two approaches. The data presented in this paper suggests that the use of AU-based analysis should consider its limited ability to reliably compare between different sessions and individuals and highlight the advantages of high-resolution sEMG for facial expression analysis.


Subject(s)
Electromyography/methods , Face/diagnostic imaging , Facial Expression , Facial Muscles/diagnostic imaging , Pattern Recognition, Automated/methods , Pattern Recognition, Visual/physiology , Adult , Electrodes , Face/anatomy & histology , Face/physiology , Facial Muscles/anatomy & histology , Facial Muscles/physiology , Female , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Male
4.
Comput Math Methods Med ; 2022: 5665972, 2022.
Article in English | MEDLINE | ID: mdl-35178115

ABSTRACT

In recent years, the performance of sports dance in China has become better and better. Naturally, the technical requirements for this dance are getting higher and higher, and the number and intensity of training have also increased, which has led to increasing injuries in sports dance. This article is based on visual sensor images to analyze and study the common injuries and prevention of sports dance practitioners. It is aimed at providing a certain reference basis for athletes' injuries, so that dance practitioners and coaches can better master sports dance training and teaching. Injury-related rules and prevention reduce the injury rate. This article puts forward the related technology of a visual sensor image and applies its technology to the prevention and research of common injuries in sports dance. At the same time, it analyzes the causes of sports dance practitioners' injuries and seeks economical and affordable massage techniques for prevention, and the method of treatment provides protection for dance practitioners. The experimental results in this article show that the Tuina group cured 15 subjects, 41 subjects were markedly effective, 13 subjects were improved, and 6 subjects were unhealed. The total effective rate was 92%.


Subject(s)
Athletic Injuries/prevention & control , Athletic Injuries/therapy , Dancing/injuries , Image Processing, Computer-Assisted/methods , Massage/methods , Adolescent , Algorithms , Athletic Injuries/diagnostic imaging , China , Computational Biology , Female , Humans , Image Processing, Computer-Assisted/instrumentation , Image Processing, Computer-Assisted/statistics & numerical data , Male , Thermography , Wavelet Analysis , Young Adult
5.
Comput Math Methods Med ; 2022: 2794326, 2022.
Article in English | MEDLINE | ID: mdl-35132329

ABSTRACT

Salp swarm algorithm (SSA) is an innovative contribution to smart swarm algorithms and has shown its utility in a wide range of research domains. While it is an efficient algorithm, it is noted that SSA suffers from several issues, including weak exploitation, convergence, and unstable exploitation and exploration. To overcome these, an improved SSA called as adaptive salp swarm algorithm (ASSA) was proposed. Thresholding is among the most effective image segmentation methods in which the objective function is described in relation of threshold values and their position in the histogram. Only if one threshold is assumed, a segmented image of two groups is obtained. But on other side, several groups in the output image are generated with multilevel thresholds. The methods proposed by authors previously were traditional measures to identify objective functions. However, the basic challenge with thresholding methods is defining the threshold numbers that the individual must choose. In this paper, ASSA, along with type II fuzzy entropy, is proposed. The technique presented is examined in context with multilevel image thresholding, specifically with ASSA. For this reason, the proposed method is tested using various images simultaneously with histograms. For evaluating the performance efficiency of the proposed method, the results are compared, and robustness is tested with the efficiency of the proposed method to multilevel segmentation of image; numerous images are utilized arbitrarily from datasets.


Subject(s)
Algorithms , Image Processing, Computer-Assisted/methods , Animals , Computational Biology , Computer Simulation , Entropy , Fuzzy Logic , Image Processing, Computer-Assisted/statistics & numerical data , Urochordata/physiology
6.
Comput Math Methods Med ; 2022: 1905151, 2022.
Article in English | MEDLINE | ID: mdl-35069776

ABSTRACT

The goal of this project is to write a program in the C++ language that can recognize motions made by a subject in front of a camera. To do this, in the first place, a sequence of distance images has been obtained using a depth camera. Later, these images are processed through a series of blocks into which the program has been divided; each of them will yield a numerical or logical result, which will be used later by the following blocks. The blocks into which the program has been divided are three; the first detects the subject's hands, the second detects if there has been movement (and therefore a gesture has been made), and the last detects the type of gesture that has been made accomplished. On the other hand, it intends to present to the reader three unique techniques for acquiring 3D images: stereovision, structured light, and flight time, in addition to exposing some of the most used techniques in image processing, such as morphology and segmentation.


Subject(s)
Gestures , Image Processing, Computer-Assisted/methods , Pattern Recognition, Automated/methods , User-Computer Interface , Computational Biology , Hand/physiology , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Imaging, Three-Dimensional/methods , Imaging, Three-Dimensional/statistics & numerical data , Movement/physiology , Pattern Recognition, Automated/statistics & numerical data , Video Recording/methods , Video Recording/statistics & numerical data
7.
Comput Math Methods Med ; 2022: 4593330, 2022.
Article in English | MEDLINE | ID: mdl-35069782

ABSTRACT

Drosophila melanogaster is an important genetic model organism used extensively in medical and biological studies. About 61% of known human genes have a recognizable match with the genetic code of Drosophila flies, and 50% of fly protein sequences have mammalian analogues. Recently, several investigations have been conducted in Drosophila to study the functions of specific genes exist in the central nervous system, heart, liver, and kidney. The outcomes of the research in Drosophila are also used as a unique tool to study human-related diseases. This article presents a novel automated system to classify the gender of Drosophila flies obtained through microscopic images (ventral view). The proposed system takes an image as input and converts it into grayscale illustration to extract the texture features from the image. Then, machine learning (ML) classifiers such as support vector machines (SVM), Naive Bayes (NB), and K-nearest neighbour (KNN) are used to classify the Drosophila as male or female. The proposed model is evaluated using the real microscopic image dataset, and the results show that the accuracy of the KNN is 90%, which is higher than the accuracy of the SVM classifier.


Subject(s)
Drosophila melanogaster/anatomy & histology , Drosophila melanogaster/classification , Machine Learning , Sex Determination Analysis/methods , Animals , Bayes Theorem , Computational Biology , Female , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Male , Microscopy , Sex Determination Analysis/statistics & numerical data , Support Vector Machine
8.
Comput Math Methods Med ; 2022: 5938493, 2022.
Article in English | MEDLINE | ID: mdl-35069786

ABSTRACT

In rhinoplasty, it is necessary to consider the correlation between the anthropometric indicators of the nasal bone, so that it prevents surgical complications and enhances the patient's satisfaction. The penetrating form of high-energy electromagnetic radiation is highly impacted on human health, which has often raised concerns of alternative method for facial analysis. The critical stage to assess nasal morphology is the nasal analysis on its anthropology that is highly reliant on the understanding of the structural features of the nasal radix. For example, the shape and size of nasal bone features, skin thickness, and also body factors aggregated from different facial anthropology values. In medical diagnosis, however, the morphology of the nasal bone is determined manually and significantly relies on the clinician's expertise. Furthermore, the evaluation anthropological keypoint of the nasal bone is nonrepeatable and laborious, also finding widely differ and intralaboratory variability in the results because of facial soft tissue and equipment defects. In order to overcome these problems, we propose specialized convolutional neural network (CNN) architecture to accurately predict nasal measurement based on digital 2D photogrammetry. To boost performance and efficacy, it is deliberately constructed with many layers and different filter sizes, with less filters and optimizing parameters. Through its result, the back-propagation neural network (BPNN) indicated the correlation between differences in human body factors mentioned are height, weight known as body mass index (BMI), age, gender, and the nasal bone dimension of the participant. With full of parameters could the nasal morphology be diagnostic continuously. The model's performance is evaluated on various newest architecture models such as DenseNet, ConvNet, Inception, VGG, and MobileNet. Experiments were directly conducted on different facials. The results show the proposed architecture worked well in terms of nasal properties achieved which utilize four statistical criteria named mean average precision (mAP), mean absolute error (MAE), R-square (R 2), and T-test analyzed. Data has also shown that the nasal shape of Southeast Asians, especially Vietnamese, could be divided into different types in two perspective views. From cadavers for bony datasets, nasal bones can be classified into 2 morphological types in the lateral view which "V" shape was presented by 78.8% and the remains were "S" shape evaluated based on Lazovic (2015). With 2 angular dimension averages are 136.41 ± 7.99 and 104.25 ± 5.95 represented by the nasofrontal angle (g-n-prn) and the nasomental angle (n-prn-sn), respectively. For frontal view, classified by Hwang, Tae-Sun, et al. (2005), nasal morphology of Vietnamese participants could be divided into three types: type A was present in 57.6% and type B was present in 30.3% of the noses. In particular, types C, D, and E were not a common form of Vietnamese which includes the remaining number of participants. In conclusion, the proposed model performed the potential hybrid of CNN and BPNN with its application to give expected accuracy in terms of keypoint localization and nasal morphology regression. Nasal analysis can replace MRI imaging diagnostics that are reflected by the risk to human body.


Subject(s)
Nasal Bone/anatomy & histology , Nasal Bone/diagnostic imaging , Neural Networks, Computer , Photogrammetry/methods , Adult , Anthropometry/methods , Computational Biology , Female , Humans , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Machine Learning/statistics & numerical data , Male , Middle Aged , Models, Anatomic , Nasal Bone/surgery , Nose/anatomy & histology , Nose/diagnostic imaging , Nose/surgery , Photogrammetry/statistics & numerical data , Rhinoplasty/methods , Rhinoplasty/statistics & numerical data , Surgery, Computer-Assisted/methods , Surgery, Computer-Assisted/statistics & numerical data , Young Adult
9.
Sci Rep ; 12(1): 1408, 2022 01 26.
Article in English | MEDLINE | ID: mdl-35082346

ABSTRACT

Magnetic resonance imaging offers unrivaled visualization of the fetal brain, forming the basis for establishing age-specific morphologic milestones. However, gauging age-appropriate neural development remains a difficult task due to the constantly changing appearance of the fetal brain, variable image quality, and frequent motion artifacts. Here we present an end-to-end, attention-guided deep learning model that predicts gestational age with R2 score of 0.945, mean absolute error of 6.7 days, and concordance correlation coefficient of 0.970. The convolutional neural network was trained on a heterogeneous dataset of 741 developmentally normal fetal brain images ranging from 19 to 39 weeks in gestational age. We also demonstrate model performance and generalizability using independent datasets from four academic institutions across the U.S. and Turkey with R2 scores of 0.81-0.90 after minimal fine-tuning. The proposed regression algorithm provides an automated machine-enabled tool with the potential to better characterize in utero neurodevelopment and guide real-time gestational age estimation after the first trimester.


Subject(s)
Brain/diagnostic imaging , Deep Learning , Gestational Age , Image Processing, Computer-Assisted/statistics & numerical data , Magnetic Resonance Imaging/standards , Neuroimaging/standards , Artifacts , Brain/growth & development , Datasets as Topic , Female , Fetus , Humans , Magnetic Resonance Imaging/methods , Neuroimaging/methods , Pregnancy , Pregnancy Trimesters/physiology , Turkey , United States
10.
Comput Math Methods Med ; 2022: 9415694, 2022.
Article in English | MEDLINE | ID: mdl-35035528

ABSTRACT

An anisotropic diffusion filtering- (ADF-) ultrasound (ADF-U) for ultrasound reconstruction was constructed based on the ADF to explore the diagnostic application of ultrasound imaging based on electronic health (E-health) for cardiac insufficiency and neuronal regulation in patients with sepsis. The 144 patients with sepsis were divided into an experimental group (78 patients with cardiac insufficiency) and a control group (66 patients with normal cardiac function), and another 58 healthy people were included in a blank control. The ultrasound examination was performed on all patients. In addition, new ultrasound image reconstruction and diagnosis were performed based on ADF and E-health, and its reconstruction effects were compared with those of the Bilateral Filter-ultrasonic (BFU) algorithm and the Wavelet Threshold-ultrasonic (WTU) algorithm. The left and right ventricular parameters and neuropeptide levels were detected and recorded. The results show that the running time, average gradient (AG), and peak signal-to-noise ratio (SNR) (PSNR) of the ADF-U algorithm were greater than those of the Bilateral Filter-ultrasonic (BFU) and Wavelet Threshold-ultrasonic (WTU), but the mean square error (MSE) was opposite (P < 0.05); the left ventricular end-systolic volume (LVESV) and the vertical distance between the mitral valve E-point to septal separation (EPSS) in the experimental group were higher than those in the control and blank group, while the left ventricular ejection fraction (LVEF), stroke volume (SV), cardiac output (CO), and left ventricular fractional shortening (LVFS) were opposite (P < 0.05); the systolic peak velocity of right ventricular free wall tricuspid annulus (Sm) and pulmonary valve blood velocity (PVBV) in the experimental group were lower than those of the control group and blank group (P < 0.05); the messenger ribonucleic acid (mRNA) of Proopiomelanocortin (POMC) and Cocain and amphetamine-regulated transcript (CART) was higher than the mRNA IN control group and blank group (P < 0.05). In short, the ADF-U algorithm proposed in this study improved the resolution, SNR, and reconstruction efficiency of E-health ultrasound images and provided an effective reference value for the diagnosis of cardiac insufficiency and neuronal adjustment analysis in patients with sepsis in the emergency department.


Subject(s)
Heart Failure/diagnostic imaging , Sepsis/diagnostic imaging , Ultrasonography/statistics & numerical data , Aged , Aged, 80 and over , Algorithms , Computational Biology , Emergency Service, Hospital , Female , Heart Failure/complications , Heart Failure/physiopathology , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Male , Middle Aged , Nervous System/diagnostic imaging , Nervous System/physiopathology , Neuropeptides/genetics , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sepsis/complications , Sepsis/physiopathology , Telemedicine/statistics & numerical data , Ventricular Function, Left , Ventricular Function, Right , Wavelet Analysis
12.
Anim Reprod Sci ; 236: 106907, 2022 Jan.
Article in English | MEDLINE | ID: mdl-34923194

ABSTRACT

This study was conducted to compare the effectiveness of two methodologies in evaluating B- and Doppler-mode ultrasonic images: analysis using ultrasonic software and utilizing a computer with ImageJ software. To determine if ImageJ software utilization is an efficacious alternative to ultrasonic software device- analysis, there were comparisons of values when using the two methods for morphological and vascular characteristics of follicular dynamics and luteal function in 18 crossbred cattle. From day 8 of an ovarian dynamics synchronization treatment regimen period until the time of ovulation (Day 10), B-mode and power-flow ultrasonic cineloops were obtained every 12 h to assess follicular diameter, wall area, and wall blood perfusion area. On Day 14 after ovulation, US cineloops of ovaries were obtained in B mode and power flow to evaluate various morphological and vascular characteristics of the corpus luteum (CL), including luteal diameter, luteal area, and CL blood perfusion area. Cineloops were evaluated and analyzed using ultrasonic software, and in a computer with ImageJ software. To evaluate consistency in results between the two methods, there was evaluation utilizing paired t-test, Pearson correlation coefficient, Bland-Altman plot, and Linear Regression Test procedures to calculate proportion of bias between values for measurements of variables evaluated. Results indicated none of the values for variables before and after ovulation differed (P > 0.05). It, therefore, was concluded that utilization of ImageJ software is an efficacious biomedical technique to analyze ultrasonic images of morphological and vascular characteristics before and after ovulation in cattle.


Subject(s)
Corpus Luteum/diagnostic imaging , Image Processing, Computer-Assisted/statistics & numerical data , Ovarian Follicle/diagnostic imaging , Software , Ultrasonics/methods , Animals , Cattle , Corpus Luteum/cytology , Female , Ovarian Follicle/cytology , Ultrasonics/instrumentation
13.
J Alzheimers Dis ; 85(3): 1251-1265, 2022.
Article in English | MEDLINE | ID: mdl-34924392

ABSTRACT

BACKGROUND: Affecting nearly half of the patients with Alzheimer's disease (AD), apathy is associated with higher morbidity and reduced quality of life. Basal ganglia and cortical atrophy have been implicated in apathy. However, the findings have varied across studies and left unclear whether subdomains of apathy may involve distinct neuroanatomical correlates. OBJECTIVE: To identify neuroanatomical correlates of AD-associated apathy. METHODS: We performed a meta-analysis and label-based review of the literature. Further, following published routines of voxel-based morphometry, we aimed to confirm the findings in an independent cohort of 19 patients with AD/mild cognitive impairment and 25 healthy controls assessed with the Apathy Evaluation Scale. RESULTS: Meta-analysis of 167 AD and 56 healthy controls showed convergence toward smaller basal ganglia gray matter volume (GMV) in apathy. Label-based review showed anterior cingulate, putamen, insula, inferior frontal gyrus (IFG) and middle temporal gyrus (MTG) atrophy in AD apathy. In the independent cohort, with small-volume-correction, right putamen and MTG showed GMVs in negative correlation with Apathy Evaluation Scale total, behavioral, and emotional scores, and right IFG with emotional score (p < 0.05 family-wise error (FWE)-corrected), controlling for age, education, intracranial volume, and depression. With the Mini-Mental State Examination scores included as an additional covariate, the correlation of right putamen GMV with behavioral and emotional score, right MTG GMV with total and emotional score, and right IFG GMV with emotional score were significant. CONCLUSION: The findings implicate putamen, MTG and IFG atrophy in AD associated apathy, potentially independent of cognitive impairment and depression, and suggest potentially distinct volumetric correlates of apathy.


Subject(s)
Alzheimer Disease/pathology , Apathy/physiology , Atrophy/pathology , Brain/pathology , Cognitive Dysfunction/pathology , Image Processing, Computer-Assisted/statistics & numerical data , Aged , Basal Ganglia/pathology , Cohort Studies , Gray Matter/pathology , Gyrus Cinguli/pathology , Humans , Magnetic Resonance Imaging , Prefrontal Cortex
14.
J Am Soc Nephrol ; 33(2): 420-430, 2022 02.
Article in English | MEDLINE | ID: mdl-34876489

ABSTRACT

BACKGROUND: In kidney transplantation, a contrast CT scan is obtained in the donor candidate to detect subclinical pathology in the kidney. Recent work from the Aging Kidney Anatomy study has characterized kidney, cortex, and medulla volumes using a manual image-processing tool. However, this technique is time consuming and impractical for clinical care, and thus, these measurements are not obtained during donor evaluations. This study proposes a fully automated segmentation approach for measuring kidney, cortex, and medulla volumes. METHODS: A total of 1930 contrast-enhanced CT exams with reference standard manual segmentations from one institution were used to develop the algorithm. A convolutional neural network model was trained (n=1238) and validated (n=306), and then evaluated in a hold-out test set of reference standard segmentations (n=386). After the initial evaluation, the algorithm was further tested on datasets originating from two external sites (n=1226). RESULTS: The automated model was found to perform on par with manual segmentation, with errors similar to interobserver variability with manual segmentation. Compared with the reference standard, the automated approach achieved a Dice similarity metric of 0.94 (right cortex), 0.90 (right medulla), 0.94 (left cortex), and 0.90 (left medulla) in the test set. Similar performance was observed when the algorithm was applied on the two external datasets. CONCLUSIONS: A fully automated approach for measuring cortex and medullary volumes in CT images of the kidneys has been established. This method may prove useful for a wide range of clinical applications.


Subject(s)
Algorithms , Image Processing, Computer-Assisted/methods , Kidney Cortex/diagnostic imaging , Kidney Medulla/diagnostic imaging , Tomography, X-Ray Computed/methods , Adult , Contrast Media , Deep Learning , Donor Selection/methods , Donor Selection/statistics & numerical data , Female , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Kidney Transplantation , Living Donors , Male , Middle Aged , Neural Networks, Computer , Observer Variation , Tomography, X-Ray Computed/statistics & numerical data
15.
Comput Math Methods Med ; 2021: 2214359, 2021.
Article in English | MEDLINE | ID: mdl-34925536

ABSTRACT

As we all know, the dietary nutrition of athletes has a great influence on physical condition and exercise ability. A good diet pattern is the basis of a reasonable diet for athletes. It helps to improve the function and physical state of athletes. This article is aimed at studying the impact of nutritious food on athletes' training and physical health. This article proposes the relevant technology of medical image recognition, which is used to study the relationship between nutritious food and the health of volleyball players and athletes, and proposes methods such as weighing method, meal review method, and measurement method, and the purpose is to exercise nutritional research and provide new ideas and methods. In addition, 200 female volleyball players were randomly selected for comparative analysis. The experimental results in this paper show that the energy intake and energy consumption of the female intervention group maintained a balance after the intervention, and there was a significant change in the negative balance state before the intervention. The energy consumption changed from -158.2 ± 156.2 to -157.2 ± 129.6. The number of athletes whose weight is close to the ideal range has increased from 44.8% to 48.5%.


Subject(s)
Physical Conditioning, Human , Sports Nutritional Physiological Phenomena , Volleyball/physiology , Adolescent , Aged , Athletes , Body Composition , Body Weight , Computational Biology , Cross-Sectional Studies , Diet , Energy Intake , Female , Humans , Image Processing, Computer-Assisted/statistics & numerical data , Nutritional Status , Prospective Studies , Surveys and Questionnaires , Young Adult
16.
PLoS Comput Biol ; 17(11): e1009063, 2021 11.
Article in English | MEDLINE | ID: mdl-34723957

ABSTRACT

A common feature of morphogenesis is the formation of three-dimensional structures from the folding of two-dimensional epithelial sheets, aided by cell shape changes at the cellular-level. Changes in cell shape must be studied in the context of cell-polarised biomechanical processes within the epithelial sheet. In epithelia with highly curved surfaces, finding single-cell alignment along a biological axis can be difficult to automate in silico. We present 'Origami', a MATLAB-based image analysis pipeline to compute direction-variant cell shape features along the epithelial apico-basal axis. Our automated method accurately computed direction vectors denoting the apico-basal axis in regions with opposing curvature in synthetic epithelia and fluorescence images of zebrafish embryos. As proof of concept, we identified different cell shape signatures in the developing zebrafish inner ear, where the epithelium deforms in opposite orientations to form different structures. Origami is designed to be user-friendly and is generally applicable to fluorescence images of curved epithelia.


Subject(s)
Cell Shape/physiology , Image Processing, Computer-Assisted/statistics & numerical data , Models, Biological , Animals , Biomechanical Phenomena , Cell Polarity , Computational Biology , Computer Simulation , Ear, Inner/embryology , Epithelium/embryology , Imaging, Three-Dimensional , Microscopy, Fluorescence , Morphogenesis , Proof of Concept Study , Software , Zebrafish/embryology
17.
Comput Math Methods Med ; 2021: 6048137, 2021.
Article in English | MEDLINE | ID: mdl-34745327

ABSTRACT

Motion blur is a common artifact in image processing, specifically in e-health services, which is caused by the motion of a camera or scene. In linear motion cases, the blur kernel, i.e., the function that simulates the linear motion blur process, depends on the length and direction of blur, called linear motion blur parameters. The estimation of blur parameters is a vital and sensitive stage in the process of reconstructing a sharp version of a motion blurred image, i.e., image deblurring. The estimation of blur parameters can also be used in e-health services. Since medical images may be blurry, this method can be used to estimate the blur parameters and then take an action to enhance the image. In this paper, some methods are proposed for estimating the linear motion blur parameters based on the extraction of features from the given single blurred image. The motion blur direction is estimated using the Radon transform of the spectrum of the blurred image. To estimate the motion blur length, the relation between a blur metric, called NIDCT (Noise-Immune Discrete Cosine Transform-based), and the motion blur length is applied. Experiments performed in this study showed that the NIDCT blur metric and the blur length have a monotonic relation. Indeed, an increase in blur length leads to increase in the blurriness value estimated via the NIDCT blur metric. This relation is applied to estimate the motion blur. The efficiency of the proposed method is demonstrated by performing some quantitative and qualitative experiments.


Subject(s)
Image Interpretation, Computer-Assisted/methods , Telemedicine/methods , Algorithms , Artifacts , Computational Biology , Computer Simulation , Fourier Analysis , Humans , Image Enhancement/methods , Image Interpretation, Computer-Assisted/statistics & numerical data , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Linear Models , Motion , Optical Phenomena , Telemedicine/statistics & numerical data
18.
Comput Math Methods Med ; 2021: 1053242, 2021.
Article in English | MEDLINE | ID: mdl-34659445

ABSTRACT

Most traditional superpixel segmentation methods used binary logic to generate superpixels for natural images. When these methods are used for images with significantly fuzzy characteristics, the boundary pixels sometimes cannot be correctly classified. In order to solve this problem, this paper proposes a Superpixel Method Based on Fuzzy Theory (SMBFT), which uses fuzzy theory as a guide and traditional fuzzy c-means clustering algorithm as a baseline. This method can make full use of the advantage of the fuzzy clustering algorithm in dealing with the images with the fuzzy characteristics. Boundary pixels which have higher uncertainties can be correctly classified with maximum probability. The superpixel has homogeneous pixels. Meanwhile, the paper also uses the surrounding neighborhood pixels to constrain the spatial information, which effectively alleviates the negative effects of noise. The paper tests on the images from Berkeley database and brain MR images from the Brain web. In addition, this paper proposes a comprehensive criterion to measure the weights of two kinds of criterions in choosing superpixel methods for color images. An evaluation criterion for medical image data sets employs the internal entropy of superpixels which is inspired by the concept of entropy in the information theory. The experimental results show that this method has superiorities than traditional methods both on natural images and medical images.


Subject(s)
Algorithms , Fuzzy Logic , Image Interpretation, Computer-Assisted/methods , Brain/diagnostic imaging , Computational Biology , Humans , Image Interpretation, Computer-Assisted/statistics & numerical data , Image Processing, Computer-Assisted/methods , Image Processing, Computer-Assisted/statistics & numerical data , Magnetic Resonance Imaging/methods , Magnetic Resonance Imaging/statistics & numerical data , Pattern Recognition, Automated/methods , Pattern Recognition, Automated/statistics & numerical data
19.
Nat Commun ; 12(1): 5992, 2021 10 13.
Article in English | MEDLINE | ID: mdl-34645817

ABSTRACT

Understanding the basis of brain function requires knowledge of cortical operations over wide spatial scales and the quantitative analysis of brain activity in well-defined brain regions. Matching an anatomical atlas to brain functional data requires substantial labor and expertise. Here, we developed an automated machine learning-based registration and segmentation approach for quantitative analysis of mouse mesoscale cortical images. A deep learning model identifies nine cortical landmarks using only a single raw fluorescent image. Another fully convolutional network was adapted to delimit brain boundaries. This anatomical alignment approach was extended by adding three functional alignment approaches that use sensory maps or spatial-temporal activity motifs. We present this methodology as MesoNet, a robust and user-friendly analysis pipeline using pre-trained models to segment brain regions as defined in the Allen Mouse Brain Atlas. This Python-based toolbox can also be combined with existing methods to facilitate high-throughput data analysis.


Subject(s)
Algorithms , Brain Mapping/methods , Cerebral Cortex/anatomy & histology , Machine Learning , Nerve Net/anatomy & histology , Optical Imaging/methods , Animals , Atlases as Topic , Cerebral Cortex/physiology , Image Processing, Computer-Assisted/statistics & numerical data , Male , Mice , Mice, Transgenic , Nerve Net/physiology , Stereotaxic Techniques
20.
Comput Math Methods Med ; 2021: 6622255, 2021.
Article in English | MEDLINE | ID: mdl-34707684

ABSTRACT

Photoacoustic imaging (PAI) is a new nonionizing, noninvasive biomedical imaging technology that has been employed to reconstruct the light absorption characteristics of biological tissues. The latest developments in compressed sensing (CS) technology have shown that it is possible to accurately reconstruct PAI images from sparse data, which can greatly reduce scanning time. This study focuses on the comparative analysis of different CS-based total variation regularization reconstruction algorithms, aimed at finding a method suitable for PAI image reconstruction. The performance of four total variation regularization algorithms is evaluated through the reconstruction experiment of sparse numerical simulation signal and agar phantom signal data. The evaluation parameters include the signal-to-noise ratio and normalized mean absolute error of the PAI image and the CPU time. The comparative results demonstrate that the TVAL3 algorithm can well balance the quality and efficiency of the reconstruction. The results of this study can provide some useful guidance for the development of the PAI sparse reconstruction algorithm.


Subject(s)
Algorithms , Diagnostic Imaging/methods , Image Processing, Computer-Assisted/statistics & numerical data , Photoacoustic Techniques/statistics & numerical data , Computational Biology , Computer Simulation , Diagnostic Imaging/statistics & numerical data , Humans , Phantoms, Imaging , Photoacoustic Techniques/instrumentation , Signal-To-Noise Ratio
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