ABSTRACT
Removing ß2-microglobulin (ß2M) from blood circulation is considered to be the most effective method to delay the occurrence of dialysis-related amyloidosis (DRA). The ideal extracorporeal ß2M removal system should be cost-effective, highly specific and having a high capacity. However, the traditional technologies based on size exclusion do not have an adequate specificity, and alternative immunosorbents have limited applications due to low capacity and their high cost. Nanobodies (Nbs), the smallest functional recombinant antibody fragments, offer several advantages to overcome these obstacles. In this study, an anti-ß2M Nb with a C-terminal thiol-tag was successfully prepared from E. coli for site-directed and oriented immobilization and usage as capture ligand in a ß2M-selective immunosorbent. The prepared immunosorbent showed a high binding capacity of up to 7 mg ß2M per mL resin, which is 17 times higher than that of previous studies using single-chain variable antibody fragments (scFv). Furthermore, an exceptional high specificity has been demonstrated as other human serum proteins were not adsorbed during dynamic adsorption experiments. About 80% of the original binding capacity of the immunosorbent was restored after four consecutive easy regenerations, whereas 90% of the original capacity was retained after 1-month storage of the resin. Moreover, the mathematical model fitted very well the in vitro perfusion. The results with this pioneering immunosorbent confirm its possible clinical application and is expected to reach the required clinical effect of immunoadsorption therapy. STATEMENT OF SIGNIFICANCE: Dialysis-related amyloidosis (DRA), associated with the accumulation of ß2-microglobulin (ß2M), is a serious complication of end-stage kidney disease. Removing ß2M from blood circulation by extracorporeal blood purification is considered to be the most effective method to delay the occurrence of DRA. However, the existing methods are incapable to eliminate sufficient quantities of ß2M from circulation, either because of lack of specificity, high cost or for low capacity. In this manuscript, we provide a practical and economic immunosorbent based on anti-ß2M nanobody for DRA. The prepared immunosorbent was reusable and storable, and demonstrated high specificity and realized a high binding capacity of up to 7 mg ß2M per mL resin, which is 17 times higher than that of the previous studies.
Subject(s)
Immunosorbents/immunology , Single-Domain Antibodies/immunology , beta 2-Microglobulin/blood , beta 2-Microglobulin/isolation & purification , Adsorption , Antibodies, Immobilized/immunology , Humans , Immunosorbent Techniques , beta 2-Microglobulin/immunologyABSTRACT
BACKGROUND: Clinical efficacy of allergen-specific Immunotherapy (AIT) towards Japanese cedar (JC) pollen allergy is firmly established but JC pollen-specific biomarker assays are lacking. Treatment-related increase of allergen-specific antibodies is a robust biomarker of successful AIT. Allergen-specific non-IgE antibodies are believed to reduce the effects of allergen exposure by competing with IgE for allergen binding, and in-vitro assays quantifying the effects of AIT-induced IgE-blocking antibodies are advantageous. A cell-free enzyme-linked immunosorbent facilitated antigen binding (ELIFAB) assay of JC pollen was established. METHODS: Serum IgE-allergen complexes were captured by immobilized recombinant CD23, and allergen-IgE-CD23 complexes were detected by a biotin-conjugated anti-human IgE antibody. Sera from JC pollen-allergic subjects without or with subcutaneous immunotherapy (SCIT) with JC pollen extract were used (n = 11/group). RESULTS: Optimal assay conditions were established at 20 µg/mL CD23 and 0.3 µg/mL JC pollen extract, and the dependency on CD23 and IgE was verified. The data show that the JC pollen ELIFAB assay is fit for purpose and demonstrates that the IgE-blocking activity is significantly increased in the JC pollen SCIT group compared with the non-treated group. CONCLUSION: The JC pollen ELIFAB assay represents a simple, cell-free biomarker assay for monitoring the development of IgE-blocking antibody activity during JC pollen AIT.
Subject(s)
Biomarkers/chemistry , Cryptomeria/immunology , Enzyme-Linked Immunosorbent Assay/methods , Immunosorbents/immunology , Pollen/immunology , Allergens/immunology , Desensitization, Immunologic/methods , Humans , Hypersensitivity/immunology , Immunoglobulin E/immunology , Immunologic Factors/immunology , Receptors, IgE/immunology , Rhinitis, Allergic, Seasonal/immunologyABSTRACT
Dialysis-related amyloidosis (DRA), which has been widely recognized to be associated with the accumulation of ß2-microglobulin (ß2-m) in blood, is one of the most common complications in patients receiving long-term dialysis treatment. The most significant side-effect of existing hemodialysis sorbents for the removal of ß2-m from blood is the loss of vital proteins due to non-specific adsorptions. Although the traditional antibodies have the capability to specifically remove ß2-m from blood, high cost limits their applications in clinics. Single domain antibodies derived from the Camelidae species serve as a superior choice in the preparation of immunoadsorbents due to their small size, high stability, amenability, simplicity of expression in microbes, and high affinity to recognize and interact with ß2-m. In this study, we modified the anti-ß2-m VHH by the formylglycine-generating enzyme (FGE), and then directly immobilized the aldehyde-modified VHH to the amino-activated beads. Notably, the fabrication is cost- and time-effective, since all the preparation steps were performed in the crude cell extract without rigorous purification. The accordingly prepared immunoadsorbent with VHHs as ligands exhibited the high capacity of ß2-m (0.75 mg/mL). In conclusion, the VHH antibodies were successfully used as affinity ligands in the preparation of novel immunoadsorbents by the site-specific immobilization, and effectively adsorbed ß2-m from blood, therefore opening a new avenue for efficient hemodialysis.
Subject(s)
Immunosorbents , Single-Domain Antibodies , beta 2-Microglobulin , Adsorption , Catalysis , Enzyme-Linked Immunosorbent Assay , Humans , Immunosorbents/immunology , Oxidoreductases Acting on Sulfur Group Donors/genetics , Oxidoreductases Acting on Sulfur Group Donors/immunology , Oxidoreductases Acting on Sulfur Group Donors/metabolism , Protein Processing, Post-Translational , Protein Stability , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Renal Dialysis/methods , Single-Domain Antibodies/genetics , Single-Domain Antibodies/immunology , Single-Domain Antibodies/metabolism , beta 2-Microglobulin/immunologyABSTRACT
The ability to detect disease markers at the single molecule level promises the ultimate sensitivity in clinical diagnosis. Fluorescence-based single-molecule analysis, however, is limited by matrix interference and can only probe a very small detection volume, which is typically not suitable for real world analytical applications. We have developed a microtiter plate immunoassay for counting single molecules of the cancer marker prostate specific antigen (PSA) using photon-upconversion nanoparticles (UCNPs) as labels that can be detected without background fluorescence. Individual sandwich immunocomplexes consisting of (1) an anti-PSA antibody immobilized to the surface of a microtiter well, (2) PSA, and (3) an anti-PSA antibody-UCNP conjugate were counted under a wide-field epifluorescence microscope equipped with a 980 nm laser excitation source. The single-molecule (digital) upconversion-linked immunosorbent assay (ULISA) reaches a limit of detection of 1.2 pg mL-1 (42 fM) PSA in 25% blood serum, which is about ten times more sensitive than commercial ELISAs, and covers a dynamic range of three orders of magnitude. This upconversion detection mode has the potential to pave the way for a new generation of digital immunoassays.
Subject(s)
Immunoassay/methods , Immunosorbents/chemistry , Limit of Detection , Prostate-Specific Antigen/analysis , Biomarkers/analysis , Immunosorbents/immunology , Luminescence , Nanoparticles/chemistryABSTRACT
BACKGROUND: Gelsolin is an actin-binding protein with several cellular functions including anti-apoptosis and is reported to have an anti-inflammatory effect. Apoptosis of keratinocytes has been implicated as a key mechanism of atopic dermatitis (AD). OBJECTIVE: We aimed to determine plasma gelsolin (pGSN) levels in children with atopic dermatitis (AD). METHOD: The diagnosis of AD was made according to Hanifin and Rajka criteria. The disease severity was scored by objective SCORAD index by the same allergist. Skin prick testing (SPT), total IgE levels, and eosinophil counts were analyzed. The pGSN levels were determined using ELISA technique. RESULTS: Children aged between 0.5 and 3.0 years were included in the study. The children with AD (AD; n = 84) were analyzed in two groups according to the presence (AD+/Atopy+; n = 54) or absence of SPT positivity (AD+/Atopy−; n = 30). The comparisons were made with a healthy control group matched for age and sex (n = 81). The median (interquartile range) of pGSN levels in AD+/A+, AD+/A− and control groups were 267 μg/ml (236-368), 293 (240-498) and 547 (361-695), respectively (p < 0.001). The difference between the control group and AD sub-groups remained significant after Bonferroni correction (p < 0.001). Correlation analysis failed to reach significance with the disease severity total IgE levels and eosinophil counts. CONCLUSION: This is the first study investigating the association of pGSN levels with AD and disease severity. pGSN levels decreased in AD. These findings suggest that gelsolin may have a role in the disease process in AD patients
No disponible
Subject(s)
Humans , Male , Female , Infant , Dermatitis, Atopic/diagnosis , Dermatitis, Atopic/etiology , Dermatitis, Atopic/pathology , Apoptosis/immunology , Apoptosis/physiology , Gelsolin/analysis , Gelsolin/immunology , Gelsolin/therapeutic use , Rhinitis, Allergic/radiotherapy , Immunosorbents/immunology , Immunosorbents/therapeutic use , Edetic Acid/analysis , Edetic Acid/immunology , Edetic Acid/therapeutic use , Cohort Studies , Prospective StudiesABSTRACT
Myeloperoxidase (MPO), a leukocyte hemoprotein released from neutrophils, is thought to be a potential participant in plaque formation and plaque rupture. Therefore, MPO is regarded as an early marker predicting the risk for atherosclerosis, especially for coronary artery disease and acute coronary syndrome. We generated hybridoma clones 1E3 and 3E8 secreting monoclonal antibodies (mAbs) specific to human MPO. BALB/c mice were immunized with MPO protein purified from human neutrophils. Splenocytes from these mice were fused with the mouse myeloma cell line SP2/0. Based on isotyping of the mAbs, both clones 1E3 and 3E8 were referred to the IgG1 subclass. The specificities of 1E3 and 3E8 were assessed by enzyme-linked immunosorbent assay (ELISA), and only 3E8 was confirmed by western blot. We developed a simple MPO-immunosorbent assay (MPO-ISA) on microplate based on both the immune activity and peroxidase activity of MPO. The mAb secreted by clone 3E8 was chosen as coating antibody to capture the plasma MPO without interfering with the peroxidase activity of MPO. Then, tetramethylbenzidine substrate was added to the microwell directly, catalyzed by captured MPO, and a colored product was formed. The simple MPO-ISA test has a sensitivity of 3.68 ng/mL. The linear concentration of MPO-ISA for commercial MPO standard ranged to 250 ng/mL. The average recovery rate is 101.02%. The imprecision within-day was <10% at three different MPO levels. The imprecision between-day was <10% at low and middle MPO levels and varied to 14.61% at the high MPO level. We found that the established MPO-ISA can detect the plasma MPO from human and cavy, but not from mouse and rat. Compared with the commercial human MPO ELISA assay, the MPO-ISA can be used to detect the natural human MPO protein, but not recombinant MPO polypeptides. The generated mAbs and MPO-ISA test may be useful tools to assess risk for inflammation and cardiac events.
Subject(s)
Antibodies, Monoclonal/immunology , Enzyme-Linked Immunosorbent Assay , Immunosorbents/immunology , Peroxidase/immunology , Animals , Antibody Specificity , Gene Expression Regulation, Enzymologic/immunology , Humans , Mice , RatsABSTRACT
Enzyme-linked immunosorbent assays (ELISA's) reported for thyroxine (T4) and 3,5,3'-triiodothyronine (T3), involved coupling of the haptens through (i) carboxylic group to carrier protein for producing antibodies and (ii) amino group to detection labels. To improve the titer and specificity of antibodies, immunogens were prepared by coupling of carboxyl group to bovine serum albumin (BSA) either directly or through adipic acid dihydrazide (ADH), after protecting amino group through acetylation of T4 and T3. Direct coupling resulted in the incorporation of 40-50 moles of T4 and T3 per BSA molecule and helped in improving immunogenic response and specificity, especially of T4. High epitope density of immunogens evoked better antibody response, since attachement of ADH as spacer, introduced 18-27 moles of haptens into carrier protein and had less effect on antibody development, with T3 being exception. Detection labels were prepared by coupling horseradish peroxidase (HRP) to amino group of thyroid hormones directly and after preparing their methyl esters, which provided sensitive displacement curves in combination with the antibodies developed against N-acetylated-T4 and T3. Unlike methyl esters, T4-HRP and T3-HRP showed higher sensitivity and seemed to be related to the affinity of the labels for binding the antibody.
Subject(s)
Enzyme-Linked Immunosorbent Assay/methods , Horseradish Peroxidase/metabolism , Immunosorbents/chemistry , Serum Albumin, Bovine/chemistry , Thyroid Hormones/analysis , Thyronines/chemistry , Thyronines/immunology , Animals , Cattle , Immunosorbents/immunology , Rabbits , Thyronines/analysisABSTRACT
In this study, we fabricated a nanopillar array of silicon oxide, involving very-large-scale integration (VLSI) and reactive ion etching (RIE), as two-dimensional periodic relief gratings (2DPRGs) on Si surfaces. Antihuman ALB was successively oriented on the pillar surface of 2DPRG modified protein G as an optical detector that is specific for targeted antigen. The antibody modified 2DPRG alone produces insignificant structure change, but upon immunocapture of antigens, the antigen filling in the 2DPRG leads to a dramatic change of the pillar scale. Binding of the antibodies to the 2DPRG occurs in a way that still allows them to function and selectively bind antigen. The performance of the sensor was evaluated by capturing HRP-human ALB on the antibody-modified 2DPRG and measuring the effective refractive index (neff) resulting from the attachment of antigens. The neff values of the 2DPRG are found to relate with the pillar scale of the 2DPRG, generated by antigen coupling, resulting in color change from pure green to orange, observed by the naked eye along an incident angle of 10-20°. Moreover, we calculated the filling factors inside the 2DPRG with effective-medium theory to verify the pillar structure changes. This technique eliminates much of the surface modifications and the secondary immunochemical or enzyme-linked steps that are common in immunoassays. Such films have potential applications as optical biosensors.
Subject(s)
Antibodies/chemistry , Antigens/chemistry , Immunoassay/instrumentation , Immunosorbents/chemistry , Antibodies/immunology , Antigens/immunology , Humans , Immunoassay/methods , Immunosorbents/immunology , Surface PropertiesABSTRACT
The sugar moiety of IgA is known to provide a link between the innate and adaptive immune systems. Terminally located glycotopes on IgA are potential ligands engaged in the interactions which may modulate the biological activities of IgA. In the present work the expressions of Maackia amurensis (MAA), Sambucus nigra (SNA), Lens culinaris (LCA), Tetragonolobus purpureus (LTA), and Ulex europaeus (UEA) reactive glycotopes on maternal plasma and amniotic IgA were evaluated in relation to the progression of a normal human pregnancy, from the 2nd trimester, throughout the 3rd trimester, perinatal period, post-date pregnancy and delivery, by lectin-IgA-ELISA, using specific biotinylated lectins. The amniotic and maternal plasma IgA concentrations and a degree of SNA and LCA reactivity of maternal plasma IgA were almost unaltered during the normal pregnancy. The amniotic IgA from the 2nd trimester was decorated by MAA-, SNA-reactive and LCA-, LTA-, and UEA-reactive glycotopes. At the turn of the 2nd and 3rd trimesters the expression of MAA-, SNA-, LTA-, and UEA-reactive glycotopes, except for LCA-reactive, increased and remained almost at unaltered levels throughout the perinatal period and delivery. However, in the post-date pregnancy the expression of LCA-, LTA-, and UEA-reactive and SNA-reactive glycotopes were significantly higher. The unique fucosylated and sialylated glycovariants of amniotic IgA associated with the progression of the normal pregnancy may illustrate a general importance of carbohydrate-lectin receptor interactions in the control and modulation of biological events to ensuring homeostasis during pregnancy, protection and well-being of fetus.
Subject(s)
Amniotic Fluid/chemistry , Fucose/chemistry , Immunoglobulin A/chemistry , N-Acetylneuraminic Acid/chemistry , Adult , Amniotic Fluid/immunology , Female , Fucose/immunology , Humans , Immunoglobulin A/immunology , Immunosorbents/immunology , Lectins/immunology , N-Acetylneuraminic Acid/immunology , PregnancyABSTRACT
Anti-A/B antibody removal from blood in the peritransplantation period facilitates ABO-incompatible transplantation and significantly increases the donor pool. We have been developing an anti-A/B immunoadsorption device (BSAF), compatible with whole blood perfusion. The BSAF is based on integrated microfiltration hollow fibers with antibody capturing beads uniformly distributed within the fiber interstitial space. In this study we fabricated BSAF prototypes, appropriately scaled down from a conceptual clinical scale device. We then, for the first time, measured the time course of anti-A capture from blood samples recirculating through the scaled down BSAF devices. We observed a significant reduction in IgM (96% ± 5%, n = 5, p < 0.001), and IgG (81% ± 18%, n = 5, p < 0.05) anti-A antibody titers within 2 h. We did not observe a significant change between the initial and final values of hematocrit, total plasma protein concentration, plasma free hemoglobin concentration, and anti-B antibody titer over five experiments. In conclusion we showed that the BSAF modules selectively removed anti-A antibodies from blood in a simple one step process, without requiring a separate plasmapheresis unit.
Subject(s)
ABO Blood-Group System/immunology , Hemofiltration/instrumentation , Hemofiltration/methods , Immunosorbents/chemistry , Immunosorbents/immunology , Isoantibodies/immunology , HumansABSTRACT
BACKGROUND: Application of immunoapheresis to eliminate pathogenic autoantibodies targeting the second extracellular loop of the ß1-receptor (ß1-AABs) is currently investigated in patients with cardiomyopathy. Aptamers (single short DNA or RNA strands) are a new class of molecules that bind to a specific target molecule. This property qualifies aptamers for potential use in the apheresis technique. We recently identified an aptamer that specifically binds to ß1-AABs, so in the present study we tested whether this aptamer could be used as a binder to prepare an apheresis column suitable for clearing ß1-AABs from rat's blood. METHODS AND RESULTS: An apheresis column was designed containing the ß1-AAB-targeting-aptamer coupled to sepharose. As tested in vitro, this column (1) binds ß1-AABs highly specifically without marked interference with common IgGs, (2) has a capacity for clearing of approximately 1L of ß1-AAB-positive serum and (3) can be completely regenerated for subsequent use. Using the column for extracorporeal apheresis of spontaneously hypertensive rats (SHR) positive for both ß1-AABs and muscarinic 2-receptor autoantibodies (M2-AABs), only ß1-AABs were removed. In a follow-up of 9 weeks, recurrence of ß1-AABs in the blood of SHR could not be detected. CONCLUSIONS: For the first time, a newly designed apheresis column with a ß1-AAB specific aptamer as a binder was successfully used to eliminate ß1-AABs from SHR blood.
Subject(s)
Aptamers, Nucleotide/chemistry , Autoantibodies , Blood Component Removal/instrumentation , Blood Component Removal/methods , Cardiomyopathies/therapy , Immunosorbents/chemistry , Receptors, Adrenergic, beta-1 , Animals , Aptamers, Nucleotide/immunology , Cardiomyopathies/blood , Cardiomyopathies/immunology , Immunosorbents/immunology , Protein Structure, Secondary , Rabbits , Rats, Inbred SHR , Receptor, Muscarinic M2/blood , Receptor, Muscarinic M2/immunologyABSTRACT
A polyclonal antiserum obtained after the immunization of a rabbit with recombinant human sperm-specific glyceraldehyde-3-phosphate dehydrogenase lacking in 68 N-terminal amino acid residues (dN-GAPDS) was purified using different immunosorbents with immobilized dN-GAPDS in the native or denatured states. The procedure resulted in isolation of two types of polyclonal antibodies. The first type interacted with native recombinant dN-GAPDS as well as with native human sperm-specific glyceraldehyde-3-phosphate dehydrogenase, not cross-reacting with muscle glyceraldehyde-3-phosphate dehydrogenase (GAPD). The second type interacted with both native and denatured forms of the sperm-specific proteins, exhibiting some cross-reaction with GAPD. Thus, the suggested approach allows isolation of the antibodies against conformational or linear epitopes from the same polyclonal serum.
Subject(s)
Antibodies/isolation & purification , Chromatography, Affinity , Animals , Antibodies/immunology , Epitopes/immunology , Glyceraldehyde-3-Phosphate Dehydrogenases/immunology , Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism , Humans , Immobilized Proteins/immunology , Immobilized Proteins/metabolism , Immunosorbents/immunology , Immunosorbents/metabolism , Male , Muscles/enzymology , Protein Denaturation , Rabbits , Spermatozoa/enzymologyABSTRACT
BACKGROUND: Complement activation was characterized during and after desensitization treatment in 19 consecutive patients receiving ABO-incompatible (ABOi) living donor kidney transplants to assess the effect of desensitization protocol including antigen-specific immunoadsorption (IA) on complement activation. METHODS: All patients received rituximab- and tacrolimus-based triple treatment. Anti-A/B antibodies were removed by IA. Serial determinations of C3, C3a, the C3a/C3 ratio, and sC5b-9 were carried out between day -30 and postoperative day 30. C1q was measured on day -30 and the day before the transplantation. In two recipients, eluates from immunoadsorbent columns were analyzed for C3a, C1q, and immunoglobulins by western blotting. Same complement analysis was performed in eluate from a control column after in vitro perfusion of AB-plasma. RESULTS: Patient and graft survival were 100% for a median follow-up of 40 months (range, 12-60 months). There were no humoral rejections based on ABO-antigen-antibody interactions. C3a and the C3a/C3 ratio declined with the start of IA treatment, and this decline was maintained postoperatively. C1q declined from day -30 to a lower value on the day before transplantation (P<0.05). In eluates from both patient and control, immunoadsorbent column immunoglobulins together with C3a and C1q were detected. CONCLUSIONS: The current protocol including antigen-specific IA interferes with the complement system; this effect may be partially responsible for the absence of humoral rejection resulting from ABO-antigen-antibody interactions and the excellent outcomes obtained after ABO-incompatible kidney transplantation.
Subject(s)
ABO Blood-Group System/immunology , Blood Group Incompatibility/immunology , Complement Activation/physiology , Desensitization, Immunologic/methods , Immunosorbents/immunology , Kidney Transplantation/immunology , Living Donors , Adult , Aged , Antibodies/blood , Cohort Studies , Complement C1q/metabolism , Complement C3/metabolism , Complement C3a/metabolism , Female , Follow-Up Studies , Graft Rejection/epidemiology , Graft Rejection/immunology , Graft Survival/immunology , Humans , Immunity, Humoral/physiology , Incidence , Male , Middle Aged , Retrospective StudiesABSTRACT
Selectivity of both peptide- and glycan-targeting antibodies was examined by 2-D LC-MS/MS. Proteins selected from biological extracts immunospecifically in a first chromatography dimension using antibodies immobilized by either covalent coupling or adsorption to protein G were desorbed with a denaturing mobile phase and transferred to a 1.5 microm nonporous particle RP chromatography (NP-RPC) column in a second dimension. Protein peak capacity of the NP-RPC column was approximately 50. Peaks collected from the RPC column were tryptic digested and the peptide fragments were identified by MALDI-MS/MS. The objective of this analytical strategy was to discriminate between protein antigens and nonantigens through identification of their peptides, leading to an evaluation of the selectivity of antibodies and immunosorbents. Quantification of the relative amount of antigen and nonantigen species captured by immunosorbents was achieved by absorbance, along with the likely capture mechanism. A limitation of the approach was in discriminating between isoforms of an antigen in which neither the antibody nor the LC-MS system targeted the differentiating feature in the isoforms.
Subject(s)
Antibodies/analysis , Antibodies/immunology , Chromatography, Liquid/methods , Immunosorbents/analysis , Immunosorbents/immunology , Tandem Mass Spectrometry/methods , Nerve Tissue Proteins/analysis , Nerve Tissue Proteins/immunologyABSTRACT
In the current study, we developed a nanosphere bio-barcode technology to detect trace gonyautoxin 2/3 (GTX 2/3). GTX 2/3-glucose oxidase (GOX) conjugates were first prepared as the coating antigen in a periodate reaction. Subsequently, gold nanoparticles (NP) dual-labeled with anti-GTX 2/3 monoclonal antibodies (Mab) and DNA oligonucleotides were synthesized via a one-step preparation method. Combining PCR with indirect competitive ELISA (icELISA), a novel immunosorbent bio-barcode assay was established utilizing the Mab-NP-dsDNA complex to convert enzymatic signals to DNA signals. Importantly, the limit of detection of the method was lower than 0.74 microg mL(-1). Thus, the immunosorbent bio-barcode assay is a rapid and high-throughput screening tool to detect GTX 2/3 in aquatic products.
Subject(s)
Enzyme-Linked Immunosorbent Assay/methods , Polymerase Chain Reaction/methods , Saxitoxin/analogs & derivatives , Antibodies, Monoclonal/immunology , Antibodies, Monoclonal/metabolism , DNA/chemistry , Glucose Oxidase/chemistry , Glucose Oxidase/metabolism , Gold/chemistry , Immunosorbents/immunology , Immunosorbents/metabolism , Metal Nanoparticles/chemistry , Saxitoxin/analysis , Saxitoxin/chemistryABSTRACT
Hemoperfusion through the granulated hemoadsorbent SCN, fibrous carbonic adsorbent AUVM and immunoadsorbent (SCN with immobilized MBP) was applied for the treatment of experimental allergic encephalomyelitis (EAE) in guinea pigs. EAE was induced by single subcutaneous injection of 100 microg of MBP in complete Freund's adjuvant. Hemoperfusion was performed on the stage of EAE manifestation or in latent period. It was found that immunoadsorbent has the highest therapeutic efficacy and allows to eliminate up to 32% anti-MBP antibodies from the serum of guinea pigs with EAE, has up to 84% and 90% of adsorptive capacity of small and middle weight endogenous substances, respectively, and reduces the level of metabolites with molecular weight less than 30 kDa in blood plasma up to 36%.
Subject(s)
Encephalomyelitis, Autoimmune, Experimental/therapy , Hemoperfusion/methods , Immunosorbents , Animals , Autoantibodies/blood , Autoantibodies/isolation & purification , Encephalomyelitis, Autoimmune, Experimental/etiology , Encephalomyelitis, Autoimmune, Experimental/immunology , Female , Guinea Pigs , Hemoperfusion/instrumentation , Immunosorbents/chemistry , Immunosorbents/immunology , Male , Myelin Basic Protein/immunology , Ovalbumin/immunology , Treatment OutcomeABSTRACT
Dilated cardiomyopathy (DCM) is a common myocardial disease characterized by ventricular dilatation and progressive depression of myocardial contractile function. Disturbances in both humoral and cellular immunity have been described among these patients. A number of antibodies against various cardiac cell proteins have been identified in DCM. Recent data indicate that cardiac antibodies play an active role in the pathogenesis of DCM, and may contribute to cardiac dysfunction of DCM patients. Therefore, removal of cardiac autoantibodies by immunoadsorption may induce hemodynamic improvement in DCM patients. Various studies with a limited number of patients indicate that immunoadsorption improves left ventricular function in DCM.
Subject(s)
Cardiomyopathy, Dilated/immunology , Cardiomyopathy, Dilated/therapy , Immunosorbents/immunology , Antibody Formation , Autoantibodies/blood , Autoantibodies/immunology , Cardiomyopathy, Dilated/physiopathology , Hemodynamics , Humans , Immunosorbent TechniquesABSTRACT
Myasthenia gravis (MG) is usually caused by autoantibodies against the human muscle acetylcholine receptor (AChR). Plasmapheresis offers a therapeutic option, but, as well as removing the pathogenic anti-AChR autoantibodies, it non-specifically removes indispensable immunoglobulins. An attractive alternative to plasmapheresis would be the extracorporeal specific removal of the autoantibodies using AChR-based immunoadsorbents. Previously, we used the N-terminal extracellular domain (ECD) of the AChR alpha subunit to immunoadsorb anti-alpha subunit autoantibodies from MG sera. In this study, we immobilised the beta -, gamma- and epsilon-AChR ECDs on Sepharose and tested them as immunoadsorbents on 50 MG sera. A given ECD removed a different percentage of autoantibodies from different sera and different ECDs removed different percentages from the same serum; on average, the beta-, gamma- and epsilon-ECDs removed 22%, 20% and 15.5% of the autoantibodies, respectively. Immunoadsorption was completed in 3 min, 1 mug of ECD removed approximately 2 pmol of autoantibodies, and the immunoadsorbent could be recycled approximately 4 times. The combined use of two (alpha+gamma) or four (alpha+beta+gamma+epsilon) ECDs in a single immunoadsorbent resulted in much higher (often additive) immunoadsorption. These results show that MG sera have autoantibodies against several AChR subunits, and suggest that the combined use of all AChR ECDs could provide the basis for a novel, antigen-specific therapy for MG.
Subject(s)
Autoantibodies/drug effects , Immunosorbents/pharmacology , Immunotherapy/methods , Myasthenia Gravis/drug therapy , Protein Subunits/pharmacology , Receptors, Nicotinic/immunology , Autoantibodies/immunology , Cell Line , Drug Combinations , Drug Synergism , Extracellular Fluid/chemistry , Humans , Immunosorbent Techniques , Immunosorbents/immunology , Immunosorbents/therapeutic use , Myasthenia Gravis/immunology , Myasthenia Gravis/physiopathology , Protein Structure, Tertiary/physiology , Protein Subunits/chemistry , Protein Subunits/immunology , Receptors, Nicotinic/therapeutic useABSTRACT
An immunosorbent assay system was integrated into a PMVS microchip. MagnaBind carboxyl derivatized beads were introduced into a microchannel, and then human immunoglobulin G (IgG) was bound to the bead surface in the microchannel of the chip. Immunoreaction was conducted in the microchannel for the bead-bounded antigen IgG with the antibody FITC-labelled IgG. On-chip detection was performed using a laser-induced fluorescence (LIF) system. The integration shortened the overall analysis time from 7 h to less than 40 min.
Subject(s)
Immunoglobulin G/analysis , Immunosorbents/immunology , Lab-On-A-Chip Devices , Microchip Analytical Procedures/methods , Microspheres , Antigens/chemistry , Antigens/immunology , Calibration , Humans , Immunoglobulin G/immunology , MicrochemistryABSTRACT
BACKGROUND: Kidney transplant recipients with a current positive complement-dependent cytotoxicity crossmatch (CDCXM) are at high risk for hyperacute rejection and graft loss. Immunoadsorption (IA) represents an efficient strategy to remove donor-specific alloantibodies. In this analysis, we evaluated effectiveness of peritransplant IA as an anti-humoral strategy to overcome a current positive CDCXM in presensitized renal allograft recipients. METHODS: Between 1999 and 2003, 40 high risk cadaveric kidney allograft recipients (median CDC panel reactive antibody [PRA] level, 77%; number of retransplants, n = 38) were subjected to peritransplant IA with protein A (one pretransplant IA session followed by a course of repeat posttransplant IA sessions) in addition to preemptive antilymphocyte antibody therapy. RESULTS: In nine of these patients, a current positive CDCXM was rendered negative by a single pretransplant IA session. Thirty-one recipients had a negative CDCXM already before pretransplant IA. No difference in graft survival was found between CDCXM-positive and CDCXM-negative recipients (3-year graft survival, 78% vs. 71%, P = 0.6). Comparable rates of immunological graft loss at 3 years were observed (11% vs. 13%, P = 0.8). Patient groups did not significantly differ with respect to median serum creatinine at 1 year (1.23 mg/dL [CDCXM-positive] vs. 1.57 mg/dl [CDCXM-negative], P = 0.07) and at the end of follow-up (median 32 months; 1.19 mg/dL vs. 1.63 mg/dL, P = 0.06). Moreover, patient groups showed similar rates of biopsy-proven cellular rejection (11% vs. 20%) or C4d-positive graft dysfunction (33% vs. 32%). CONCLUSION: Our results demonstrate that peritransplant IA enables successful cadaveric kidney transplantation in the context of a positive CDCXM.