ABSTRACT
Oxylipins are powerful signalling compounds derived from polyunsaturated fatty acids (PUFAs) and involved in regulating the immune system response. A mass spectrometry-based method was developed and validated for the targeted profiling of 52 oxylipins (e.g., isoprostanoids, prostaglandins, epoxy- and hydroxy-fatty acids, specialized pro-resolving mediators) and 4 PUFAs in small urinary extracellular vesicles (uEVs). Ultrasound-assisted extraction using a 50:50 v/v MeOH:H2O mixture ensured optimal analytical performances. Limits of detection ranged between 10 and 400 pg/mL for oxylipins and 0.10-3 ng/mL for PUFAs. Satisfactory recoveries (85-116 %) and good intra- and inter-day precisions (RSD ≤15 %) were obtained for all the analytes. The reliability of the procedure was tested in a real case scenario by monitoring ultramarathon runners during the world Tor des Géants® (TDG) race. Both F2- and E2-isoprostanes were detected in small uEVs of the ultramarathon runners, suggesting the onset of an oxidant insult. 5-F2t-IsoP exhibited significant pre- to post-race variations, thus potentially representing a non-invasive marker of in-vivo lipid peroxidation. The presence of specialized pro-resolving mediators suggests the activation of pro-resolution signalling cascade resolving inflammation. These outcomes may help manage post-exercise recovery and improve training.
Subject(s)
Extracellular Vesicles , Isoprostanes , Running , Tandem Mass Spectrometry , Humans , Tandem Mass Spectrometry/methods , Extracellular Vesicles/chemistry , Chromatography, High Pressure Liquid/methods , Isoprostanes/urine , Isoprostanes/analysis , Male , Adult , Fatty Acids, Unsaturated/analysis , Fatty Acids, Unsaturated/urine , Oxylipins/analysis , Oxylipins/urine , Solid Phase Microextraction , Middle AgedABSTRACT
Isoprostanes are a large group of compounds formed as products of free radical oxidation of polyunsaturated fatty acids, which are isomers of prostaglandin. They are present in all body tissues and biological fluids in quantifiable concentrations. Since 2018, the determination of isoprostanes by chromatographic technique with mass spectrometry is the golden standard of the oxidative stress markers determination in relation to oxidative damage to lipids. The publication is a synthetic review of recently published articles on the use of isoprostanes as a marker of lipid peroxidation determined with the liquid chromatography with tandem mass spectrometry technique. It presents the results of research using isoprostanes as a marker in medicine, in monitoring people working in exposure to harmful substances and in lifestyle research. Med Pr. 2023;74(2):119-25.
Subject(s)
Isoprostanes , Tandem Mass Spectrometry , Humans , Chromatography, Liquid/methods , Tandem Mass Spectrometry/methods , Isoprostanes/analysis , Lipid Peroxidation , Oxidative Stress , Biomarkers/analysisABSTRACT
BACKGROUND: The Academy of Breastfeeding Medicine published a clinical protocol for Human Milk storage, recommending refrigeration at a temperature of 4 °C up to 4 d as the optimal conditions for the safety and bactericidal capacity of Human Milk. However, few studies were conducted to evaluate the change in milk composition during this type of refrigeration storage. AIM: To elucidate some uncertainties regarding the Human Milk composition and prolonged cold storage, we have investigated the effects of storage at 4 °C up to 96 h on an important category of oxidative stress markers: the Isoprostanes (F2-isoprostanes, F4-neuroprostanes and F3-isoprostanes). MATERIAL AND METHOD: The experiment was repeated 3 times to ensure reproducibility of the results. We enrolled 3 donating healthy mothers for each time (total: 9 mothers). Milk was collected with standard extraction methods. Immediately after collection, each Human Milk sample from each mother was pooled and then divided into 5 aliquots. One aliquot (0 h) was immediately frozen at -80 °C until the analysis. The other aliquots (24 h, 48 h, 72 h, 96 h) were stored in a refrigerator at 4 °C respectively for 24, 48, 72 and 96 h, then immediately frozen at -80 °C until the analysis. Milk samples were then used to determine concentration of Isoprostanes in Liquid Chromatography - Mass Spectrometry and Liquid Chromatography - Tandem Mass Spectrometry. RESULTS: Isoprostanes were detectable in all Human Milk samples. There was no significant trend of the concentration of the tested analytes over time. DISCUSSION AND CONCLUSION: This study provides evidence of the presence in human milk of all the tested isoprostanes: in particular, F2-isoprostanes, F4-neuroprostanes and F3-isoprostanes. Refrigeration and storage of fresh Human Milk in controlled conditions for 96 h did not significantly affect its bioactivity and nutritional quality related with these biomarkers.
Subject(s)
Neuroprostanes , Refrigeration , Humans , Isoprostanes/analysis , F2-Isoprostanes/analysis , Milk, Human/chemistry , Neuroprostanes/analysis , Reproducibility of Results , Biomarkers/analysisABSTRACT
Biomarkers of oxidative stress are generally measured in blood and its derivatives. However, the invasiveness of blood collection makes the monitoring of such chemicals during exercise not feasible. Saliva analysis is an interesting approach in sport medicine because the collection procedure is easy-to-use and does not require specially-trained personnel. These features guarantee the collection of multiple samples from the same subject in a short span of time, thus allowing the monitoring of the subject before, during and after physical tests, training or competitions. The aim of this work was to evaluate the possibility of following the changes in the concentration of some oxidative stress markers in saliva samples taken over time by athletes under exercise. To this purpose, ketones (i.e. acetone, 2-butanone and 2-pentanone), aldehydes (i.e. propanal, butanal, and hexanal), α,ß-unsaturated aldehydes (i.e. acrolein and methacrolein) and di-carbonyls (i.e. glyoxal and methylglyoxal) were derivatized with 2,4-dinitrophenylhydrazine, and determined by ultra-high performance liquid chromatography coupled to diode array detector. Prostaglandin E2, F2/E2-isoprostanes, F2-dihomo-isoprostanes, F4-neuroprostanes, and F2-dihomo-isofuranes were also determined by a reliable analytical procedure that combines micro-extraction by packed sorbent and ultra-high performance liquid chromatography-electrospray ionization tandem mass spectrometry. Overall the validation process showed that the methods have limits of detection in the range of units of ppb for carbonyls and tens to hundreds of ppt for isoprostanes and prostanoids, very good quantitative recoveries (90-110%) and intra- and inter-day precision lower than 15%. The proof of applicability of the proposed analytical approach was investigated by monitoring the selected markers of oxidative stress in ten swimmers performing a VO2max cycle ergo meter test. The results highlighted a clear increase of salivary by-products of oxidative stress during exercise, whereas a sharp decrease, approaching baseline values, of these compounds was observed in the recovery phase. This study opens up a new approach in the evaluation of oxidative stress and its relation to aerobic activity.
Subject(s)
Exercise Test , Glyoxal/analysis , Isoprostanes/analysis , Prostaglandins/analysis , Saliva/chemistry , Swimming/physiology , Adult , Athletes , Biomarkers/analysis , Female , Humans , Male , Oxidative Stress/physiology , Young AdultABSTRACT
Traditional medicine may not control bronchial asthma. Many patients have uncontrolled symptoms and the underlying ongoing inflammation is persistent. OBJECTIVE: to assess efficacy of laser acupuncture in improving asthma symptoms and underlying oxidative stress through monitoring exhaled 8-isoprostane. METHOD: 48 asthmatic (case group) received successive low level laser acupuncture sessions to stimulate acupoints for chronic asthma and 24 asthmatics received deactivated laser acupuncture sessions (control group). Asthma symptoms, asthma control questionnaire, concentration of 8-Isoprostane in exhaled breath condensate and airway resistance were assessed before and after laser acupuncture therapy. RESULTS: After the completion of the course of laser acupuncture therapy, we observed significant improvement of asthma symptoms. Asthma control questionnaire improved from 9.7⯱â¯3.3 to 21.8⯱â¯3.6 (p 0.001). EBC 8-Isoprostane dropped from 14.7⯱â¯5.4 to 8.1⯱â¯5.0 (p 0.001). The airway resistance at R5 and R20 significantly decreased from 116.6⯱â¯25.8 & 124.5⯱â¯31.2 to 101.5⯱â¯25.6 &110.9⯱â¯29.9 respectively (p 0.001). Control patients who received sham acupuncture therapy did not show such improvement. CONCLUSION: Laser acupuncture is an effective modality in treating bronchial asthma as evidenced by improved symptoms, airway resistance, and oxidative biomarkers.
Subject(s)
Acupuncture Therapy/methods , Asthma/diagnosis , Asthma/therapy , Isoprostanes/analysis , Laser Therapy/methods , Asthma/etiology , Biomarkers/analysis , Breath Tests , Humans , Oxidative StressABSTRACT
BACKGROUND: Clinical data indicate that airway inflammation in children with cystic fibrosis (CF) arises early, is associated with structural lung damage, and predicts progression. In bronchoalveolar lavage fluid (BALF) from CFTR mutant mice, several aspects of lipid metabolism are abnormal that contributes to lung disease. We aimed to determine whether lipid pathway dysregulation is also observed in BALF from children with CF, to identify biomarkers of early lung disease and potential therapeutic targets. METHODS: A comprehensive panel of lipids that included Sphingolipids, oxylipins, isoprostanes and lysolipids, all bioactive lipid species known to be involved in inflammation and tissue remodeling, were measured in BALF from children with CF (1-6â¯years, Nâ¯=â¯33) and age-matched non-CF patients with unexplained inflammatory disease (Nâ¯=â¯16) by HPLC-MS/MS. Lipid data were correlated with chest CT scores and BALF inflammation biomarkers. RESULTS: The ratio of long chain to very long chain ceramide species (LCC/VLCC) and lysolipid levels were enhanced in CF compared to non-CF patients, despite comparable neutrophil counts and bacterial load. In CF patients both LCC/VLCC and lysolipid levels correlated with inflammation and chest CT scores. The ceramide precursors Sphingosine, Sphinganine, Sphingomyelin, correlated with inflammation, whilst the oxidative stress marker isoprostane correlated with inflammation and chest CT scores. No correlation between lipids and current bacterial infection in CF (Nâ¯=â¯5) was observed. CONCLUSIONS: Several lipid biomarkers of early CF lung disease were identified, which point toward potential disease monitoring and therapeutic approaches that can be used to complement CFTR modulators.
Subject(s)
Bronchoalveolar Lavage Fluid/immunology , Cystic Fibrosis , Isoprostanes , Lung , Oxidative Stress/immunology , Oxylipins , Sphingolipids , Biomarkers/analysis , Biomarkers/metabolism , Cell Count/methods , Child, Preschool , Cystic Fibrosis/diagnosis , Cystic Fibrosis/immunology , Cystic Fibrosis/metabolism , Disease Progression , Female , Humans , Inflammation/metabolism , Isoprostanes/analysis , Isoprostanes/metabolism , Lipidomics/methods , Lung/immunology , Lung/metabolism , Male , Oxylipins/analysis , Oxylipins/metabolism , Sphingolipids/analysis , Sphingolipids/metabolism , Tandem Mass Spectrometry/methodsABSTRACT
The evolutionary history of hominins has been characterized by significant dietary changes, which include the introduction of meat eating, cooking, and the changes associated with plant and animal domestication. The Western pattern diet has been linked with the onset of chronic inflammation, and serious health problems including obesity, metabolic syndrome, and cardiovascular diseases. Diets enriched with ω-3 marine PUFAs have revealed additional improvements in health status associated to a reduction of proinflammatory ω-3 and ω-6 lipid mediators. Lipid mediators are produced from enzymatic and non-enzymatic oxidation of PUFAs. Interest in better understanding the occurrence of these metabolites has increased exponentially as a result of the growing evidence of their role on inflammatory processes, control of the immune system, cell signaling, onset of metabolic diseases, or even cancer. The scope of this review has been to highlight the recent findings on: a) the formation of lipid mediators and their role in different inflammatory and metabolic conditions, b) the direct use of lipid mediators as antiinflammatory drugs or the potential of new drugs as a new therapeutic option for the synthesis of antiinflammatory or resolving lipid mediators and c) the impact of nutritional interventions to modulate lipid mediators synthesis towards antiinflammatory conditions. In a second part, we have summarized methodological approaches (Lipidomics) for the accurate analysis of lipid mediators. Although several techniques have been used, most authors preferred the combination of SPE with LC-MS. Advantages and disadvantages of each method are herein addressed, as well as the main LC-MS difficulties and challenges for the establishment of new biomarkers and standardization of experimental designs, and finally to deepen the study of mechanisms involved on the inflammatory response.
Subject(s)
Cardiovascular Diseases/metabolism , Fatty Acids, Omega-3/metabolism , Fatty Acids, Omega-6/metabolism , Lipidomics/methods , Metabolic Syndrome/metabolism , Obesity/metabolism , Biomarkers/analysis , Cardiovascular Diseases/diagnosis , Cardiovascular Diseases/diet therapy , Cardiovascular Diseases/physiopathology , Chromatography, Liquid , Diet/methods , Dietary Fats/administration & dosage , Dietary Fats/metabolism , Fatty Acids, Omega-3/administration & dosage , Fatty Acids, Omega-3/chemistry , Fatty Acids, Omega-6/administration & dosage , Fatty Acids, Omega-6/chemistry , Humans , Inflammation , Isoprostanes/analysis , Isoprostanes/chemistry , Isoprostanes/metabolism , Lipid Peroxides/analysis , Lipid Peroxides/chemistry , Lipid Peroxides/metabolism , Lipidomics/instrumentation , Mass Spectrometry , Metabolic Syndrome/diagnosis , Metabolic Syndrome/diet therapy , Metabolic Syndrome/physiopathology , Obesity/diagnosis , Obesity/diet therapy , Obesity/physiopathology , Prostaglandins/analysis , Prostaglandins/chemistry , Prostaglandins/metabolism , Thromboxanes/analysis , Thromboxanes/chemistry , Thromboxanes/metabolismABSTRACT
Preterm newborns have an immature antioxidant defense system and are especially susceptible to oxidative stress. Resuscitation, mechanical ventilation, intermittent hypoxia and apneic episodes require frequently oxygen supplementation which leads to oxidative stress in preterm newborns. The consequences of oxidative damage are increased short and long-term morbidities, neurodevelopmental impairment and increased mortality. Oxidative stress biomarkers are determined in blood samples from preterm children during their stay in neonatal intensive care units especially for research purposes. However, there is a tendency towards reducing invasive and painful techniques in the NICU (Neonatal Intensive Care Unit) and avoiding excessive blood extractions procedures. In this paper, it has been described some studies that employed non-invasive samples to determine oxidative stress biomarkers form preterm infants in order to perform a close monitoring biomarker with a significant greater predictive value. Among these methods we describe a previously developed and validated high-performance liquid chromatography tandem mass spectrometry method that allow to accurately determine the most reliable biomarkers in biofluids, which are non-invasively and painlessly obtained.
Subject(s)
Bronchopulmonary Dysplasia/diagnosis , Enterocolitis, Necrotizing/diagnosis , Oxidative Stress , Reactive Oxygen Species/analysis , Retinopathy of Prematurity/diagnosis , Biomarkers/analysis , Bronchopulmonary Dysplasia/blood , Bronchopulmonary Dysplasia/urine , Chromatography, High Pressure Liquid , Enterocolitis, Necrotizing/blood , Enterocolitis, Necrotizing/urine , Female , Fetus , Humans , Infant, Newborn , Infant, Premature , Isoprostanes/analysis , Pregnancy , Retinopathy of Prematurity/blood , Retinopathy of Prematurity/urine , Saliva/chemistry , Tandem Mass Spectrometry , Thiobarbituric Acid Reactive Substances/analysis , Vitamins/analysisABSTRACT
PURPOSE: Lipid mediators of inflammation are a group of signaling molecules produced by various cells under physiological conditions and modulate the inflammatory process during various pathologic conditions. Although eicosanoids and F2-isoprostanes are recognized lipid mediators of inflammation, there is no consensus yet on the extraction and mass spectrometry (MS) method for their analysis in individual human tear samples. Thus, the aim of this study was to develop an optimal method for extraction of lipid mediators of inflammation in the tear film and evaluate MS techniques for their analysis. METHODS: Basal tears were collected from each eye of 19 subjects using glass microcapillaries. Lipid extraction was performed using either varying concentrations of acidified methanol, a modified Folch method, or solid-phase extraction. Initially, an untargeted analysis of the extracts was performed using SCIEX TripleTOF 5600 mass spectrometer to identify any lipid mediators of inflammation (eicosanoids) and later a targeted analysis was performed using the SCIEX 6500 Qtrap to identify and quantify prostaglandins and isoprostanes. Mass spectra and chromatograms were analyzed using Peakview, XCMS, and Multiquant software. RESULTS: Prostaglandins and isoprostanes were observed and quantified using the Qtrap mass spectrometer under multiple reaction monitoring (MRM) mode after solid-phase extraction. Extraction with acidified methanol along with the Folch method produced cleaner spectra during MS with the Triple time of flight (TOF) mass spectrometer. Lipid mediators of inflammation were not observed in any of the tear samples using the Triple TOF mass spectrometer. CONCLUSIONS: Solid-phase extraction may be the method of choice for extraction of prostaglandins and isoprostanes in low volumes of tears. The SCIEX Qtrap 6500 in MRM mode may be suitable to identify and quantify similar lipid mediators of inflammation.
Subject(s)
Eicosanoids/analysis , Inflammation Mediators/analysis , Isoprostanes/analysis , Tears/chemistry , Chromatography, High Pressure Liquid , Female , Humans , Male , Mass SpectrometryABSTRACT
Misregulation of oxidative and antioxidative processes in the organism - oxidative stress - contributes to the pathogenesis of different diseases, e.g. inflammatory or neurodegenerative diseases. Oxidative stress leads to autoxidation of polyunsaturated fatty acids giving rise to prostaglandin-like isoprostanes (IsoP) and isofurans (IsoF). On the one hand they could serve as biomarker of oxidative stress and on the other hand may act as lipid mediators, similarly as the enzymatically formed oxylipins. In the present paper we describe the development of an LC-ESI(-)-MS/MS method allowing the parallel quantification of 27 IsoP and 8 IsoF derived from 6 different PUFA (ALA, ARA, EPA, AdA, n6-DPA, DHA) within 12 min. The chromatographic separation was carried out on an RP-C18 column (2.1 × 150 mm, 1.8 µm) yielding narrow peaks with an average width at half maximum of 3.3-4.2 s. Detection was carried out on a triple quadrupole mass spectrometer operating in selected reaction monitoring mode allowing the selective detection of regioisomers. The limit of detection ranged between 0.1 and 1 nM allowing in combination with solid phase extraction the detection of IsoP and IsoF at subnanomolar concentrations in biological samples. The method was validated for human plasma showing high accuracy and precision. Application of the approach on the investigation of oxidative stress in cultured cells indicated a distinct pattern of IsoP and IsoF in response to reactive oxygen species which warrants further investigation. The described method is not only the most comprehensive approach for the simultaneous quantification of IsoP and IsoF, but it was also integrated in a targeted metabolomics method (Ostermann et al. (2015) Anal Bioanal Chem) allowing the quantification of in total 164 oxylipins formed enzymatically and non-enzymatically within 30.5 min.
Subject(s)
Fatty Acids, Omega-3/chemistry , Fatty Acids, Omega-6/chemistry , Furans/analysis , Isoprostanes/analysis , Chromatography, Liquid , Fatty Acids, Omega-3/blood , Fatty Acids, Omega-3/metabolism , Fatty Acids, Omega-6/blood , Fatty Acids, Omega-6/metabolism , Furans/metabolism , HCT116 Cells , Humans , Isoprostanes/metabolism , Molecular Structure , Oxidative Stress , Solid Phase Extraction , Spectrometry, Mass, Electrospray Ionization , Tandem Mass SpectrometryABSTRACT
Isoprostanes (IsoPs) are a class of oxidation products naturally formed in vivo that are indicative of endogenous oxidative stress. In individuals with chronic and oxidative stress related diseases, IsoPs are increased to pathological levels. Since they are excreted through urine into sewage systems, IsoPs can be detected in wastewater treatment plants' (WWTPs) effluents and thus can be used to evaluate the health status of a given population. The underlying principle is that higher isoprostanes WWTPs' levels correspond to populations undergoing higher levels of oxidative stress, and thus disease. However, IsoPs are not eliminated by WWTPs and will end up being released into the aquatic environment, where they will be available for uptake by aquatic species. Being bioactive molecules, it has been suggested that IsoPs in the environment may elicit oxidative stress in aquatic organisms. In this context, we have critically reviewed the available data on IsoPs as products and effectors of toxicity, and propose the new concept of "circular toxicity". In general, IsoPs excreted by humans as a consequence of oxidative stress are released into the aquatic environment where they may interact with aquatic organisms and induce the production of more IsoPs. These stress markers, in turn, will also be excreted, increasing the already high levels of stressors in the aquatic environment and thus create an escalating cycle of oxidative stress.
Subject(s)
Aquatic Organisms/drug effects , Isoprostanes/analysis , Isoprostanes/toxicity , Oxidative Stress/physiology , Wastewater/chemistry , Animals , Biomarkers/analysis , Humans , Lipid Peroxidation/physiology , Oxidation-ReductionABSTRACT
AIM: The aim of this meta-analysis was to investigate associations between idiopathic pulmonary fibrosis (IPF) and markers of oxidative stress (OS) measured in different biological samples. METHODS: A systematic search of publications listed in PubMed, Web of Science, Scopus and Google Scholar from inception to December 2017 was conducted. RESULTS: Significant differences between IPF patients and controls were observed for all biomarkers (thiobarbituric acid reactive substances, hydroperoxides and glutathione), barring systemic isoprostanes. CONCLUSION: This meta-analysis showed a consistent increase in the concentrations of OS markers or a reduction in antioxidant markers, in patients with IPF, independent of the type of biological sample. Pending the results of further studies, OS biomarkers might be useful for the diagnosis and monitoring of IPF.
Subject(s)
Biomarkers/metabolism , Idiopathic Pulmonary Fibrosis/diagnosis , Oxidative Stress , Antioxidants/metabolism , Biomarkers/blood , Bronchoalveolar Lavage Fluid/chemistry , Glutathione/metabolism , Humans , Idiopathic Pulmonary Fibrosis/metabolism , Isoprostanes/analysis , Protein CarbonylationABSTRACT
Hazelnut and cocoa spread is an Italian product containing cocoa and hazelnut. Several epidemiological studies suggest that cocoa and hazelnuts cocoa exert beneficial cardiovascular effects. To investigate whether in smokers, hazelnut and cocoa spread elicits artery dilatation via down-regulation of oxidative stress. Flow-mediated dilatation (FMD), oxidative stress (as assessed by serum isoprostanes excretion, Nox2 activation and NO bioavailability) and antioxidant status [as assessed by vitamin E levels, plasma total polyphenols and H2O2 breaking down activity (HBA)] were studied in 20 smokers in a crossover, single-blind study. Patients were randomly allocated to 60 g of Hazelnut and cocoa spread or 60 g of milk chocolate (≤ 35% cocoa). FMD, serum isoprostanes, Nox2 activation, NOx, vitamin E, HBA and total polyphenols were assessed at baseline and 2 h after chocolate ingestion. After Hazelnut and cocoa spread intake, FMD and NOx significantly increased (from 4.3 ± 2.8 to 8.0 ± 3.2%, p < 0.001 and from 23.1 ± 5.5 to 32.0 ± 12.6 µM, p = 0.016, respectively); conversely, serum isoprostanes and Nox2 activation significantly decreased (from 302.8 ± 59.8 to 240.7 ± 90.8 pmol/l, p = 0.03 and from 25 ± 4.4 to 22.6 ± 3.2, p = 0.03, respectively). After Hazelnut and cocoa spread intake, serum total polyphenols, vitamin E and HBA significantly increased (from 133.8 ± 49.7 to 202.5 ± 69.5 mg/l GAE, p = 0.001; from 3.56 ± 1.4 to 4.5 ± 1.0 µmol/mmol cholesterol, p = 0.002 and from 63.3 ± 13.2 to 74.2 ± 12.4%, p = 0.003, respectively). No changes in the above variables were observed after milk chocolate intake. A linear correlation analysis shows that Δ (expressed by difference of values between before and after chocolate intake) of FMD correlates with Δ of total polyphenols and Δ of vitamin E. This study shows that Hazelnut and cocoa spread improves FMD with a mechanism potentially involving downregulation of oxidative stress and eventually increased NO generation in smokers.
Subject(s)
Arteries/drug effects , Chocolate , Corylus , Dilatation/methods , Smokers/statistics & numerical data , Adult , Analysis of Variance , Biological Availability , Female , Humans , Isoprostanes/analysis , Isoprostanes/blood , Italy , Male , NADPH Oxidase 2/analysis , NADPH Oxidase 2/blood , Nitrous Oxide/analysis , Nitrous Oxide/blood , Oxidative Stress , Polyphenols/chemistry , Polyphenols/classification , Polyphenols/therapeutic use , Statistics, Nonparametric , Vitamin E/chemistry , Vitamin E/classification , Vitamin E/therapeutic useABSTRACT
Oxidative stress and inflammation are underlying pathogenic mechanisms associated with the progression of several pathological conditions and immunological responses. Elucidating the role of signalling lipid classes, which include, among others, the isoprostanes, nitro fatty acids, prostanoids, sphingoid bases and lysophosphatidic acids, will create a snapshot of the cause and effect of inflammation and oxidative stress at the metabolic level. Here we describe a fast, sensitive, and targeted ultra-high-performance liquid chromatography-tandem mass spectrometry metabolomics method that allows the quantitative measurement and biological elucidation of 17 isoprostanes as well as their respective isomeric prostanoid mediators, three nitro fatty acids, four sphingoid mediators, and 24 lysophosphatidic acid species from serum as well as organ tissues, including liver, lung, heart, spleen, kidney and brain. Application of this method to paired mouse serum and tissue samples revealed tissue- and serum-specific stress and inflammatory readouts. Little correlation was found between localized (tissue) metabolite levels compared with the systemic (serum) circulation in a homeostatic model. The application of this method in future studies will enable us to explore the role of signalling lipids in the metabolic pathogenicity of stress and inflammation during health and disease.
Subject(s)
Inflammation/metabolism , Metabolome , Metabolomics/methods , Nitrosative Stress , Oxidative Stress , Animals , Chromatography, High Pressure Liquid/methods , Fatty Acids/analysis , Fatty Acids/metabolism , Humans , Isoprostanes/analysis , Isoprostanes/metabolism , Lysophospholipids/analysis , Lysophospholipids/metabolism , Male , Mice, Inbred C57BL , Tandem Mass Spectrometry/methodsABSTRACT
Oxygenated lipid products of non-cyclooxygenase derivatives, namely, prostanoids such as, isoprostanes and isofurans, are formed in vivo through lipid autoxidation. Insofar it has been marked as novel biomarkers of oxidative stress in the biological systems. Elevations of these oxidized products are associated with several diseases. This chapter describes the preparation and measurement of the products, including newly identified F2-dihomo-isoprostanes and dihomo-isofurans, from plasma and tissue samples using the liquid chromatography-tandem mass spectrometry approach.
Subject(s)
Chromatography, Liquid/methods , Furans/analysis , Isoprostanes/analysis , Tandem Mass Spectrometry/methods , Biomarkers/analysis , Blood Chemical Analysis , Humans , Lipid Peroxidation , Oxidative StressABSTRACT
Oxidant stress has been identified as important in the pathology of many diseases. Oxidation products of polyunsaturated fatty acids collectively termed isoprostanes, neuroprostanes, and isofurans are considered the most reliable measures of in vivo lipid oxidation, and they are widely used to assess oxidant stress in various diseases. Here we describe the measurement of these lipid oxidation products using gas chromatography mass spectrometry with electron capture negative ionization.
Subject(s)
Furans/analysis , Gas Chromatography-Mass Spectrometry/methods , Isoprostanes/analysis , Neuroprostanes/analysis , Biomarkers/analysis , Furans/blood , Furans/urine , Humans , Isoprostanes/blood , Isoprostanes/urine , Lipid Peroxidation , Neuroprostanes/blood , Neuroprostanes/urine , Oxidative StressABSTRACT
Introduction: Homocysteine (Hcy) tissue accumulation occurs in a metabolic disease characterized biochemically by cystathionine ß-synthase (CBS) deficiency and clinically by mental retardation, vascular problems, and skeletal abnormalities. Previous studies indicate the occurrence of DNA damage secondary to hyperhomocysteinemia and it was observed that DNA damage occurs in leukocytes from CBS-deficient patients. This study aimed to investigate whether an oxidative mechanism could be involved in DNA damage previously found and investigated the in vitro effect of N-acety-L-cysteine (NAC) on DNA damage caused by high Hcy levels. Methods: We evaluated a biomarker of oxidative DNA damage in the urine of CBSdeficient patients, as well as the in vitro effect of NAC on DNA damage caused by high levels of Hcy. Moreover, a biomarker of lipid oxidative damage was also measured in urine of CBS deficient patients. Results: There was an increase in parameters of DNA (8-oxo-7,8-dihydro-2'- deoxyguanosine) and lipid (15-F2t-isoprostanes levels) oxidative damage in CBS-deficient patients when compared to controls. In addition, a significant positive correlation was found between 15-F2t-isoprostanes levels and total Hcy concentrations. Besides, an in vitro protective effect of NAC at concentrations of 1 and 5 mM was observed on DNA damage caused by Hcy 50 µM and 200 µM. Additionally, we showed a decrease in sulfhydryl content in plasma from CBS-deficient patients when compared to controls. Discussion: These results demonstrated that DNA damage occurs by an oxidative mechanism in CBS deficiency together with lipid oxidative damage, highlighting the NAC beneficial action upon DNA oxidative process, contributing with a new treatment perspective of the patients affected by classic homocystinuria.
Subject(s)
Humans , Female , Child , Adolescent , Adult , Young Adult , Acetylcysteine/pharmacology , DNA Damage , Oxidative Stress , Cystathionine/metabolism , Deoxyguanosine/urine , Homocystinuria/genetics , Antioxidants/pharmacology , Biomarkers/urine , Case-Control Studies , Creatinine/urine , Comet Assay , Cystathionine/biosynthesis , Cystathionine/blood , Isoprostanes/analysis , Deoxyguanosine/analogs & derivatives , Homocysteine/blood , Homocystinuria/bloodABSTRACT
INTRODUCTION: Rat mesenchymal stem cells (rMSCs) labeled with 1) poly-l-lysine-coated superparamagnetic iron oxide nanoparticles or 2) silica-coated cobalt-zinc-iron nanoparticles were implanted into the left brain hemisphere of rats, to assess their effects on the levels of oxidative damage to biological macromolecules in brain tissue. METHODS: Controls were implanted with unlabeled rMSCs. Animals were sacrificed 24 hours or 4 weeks after the treatment, and the implantation site along with the surrounding tissue was isolated from the brain. At the same intervals, parallel groups of animals were scanned in vivo by magnetic resonance imaging (MRI). The comet assay with enzymes of excision DNA repair (endonuclease III and formamidopyrimidine-DNA glycosylase) was used to analyze breaks and oxidative damage to DNA in the brain tissue. Oxidative damage to proteins and lipids was determined by measuring the levels of carbonyl groups and 15-F2t-isoprostane (enzyme-linked immunosorbent assay). MRI displayed implants of labeled cells as extensive hypointense areas in the brain tissue. In histological sections, the expression of glial fibrillary acidic protein and CD68 was analyzed to detect astrogliosis and inflammatory response. RESULTS: Both contrast labels caused a similar response in the T2-weighted magnetic resonance (MR) image and the signal was clearly visible within 4 weeks after implantation of rMSCs. No increase of oxidative damage to DNA, lipids, or proteins over the control values was detected in any sample of brain tissue from the treated animals. Also, immunohistochemistry did not indicate any serious tissue impairment around the graft. CONCLUSION: Both tested types of nanoparticles appear to be prospective and safe labels for tracking the transplanted cells by MR.
Subject(s)
Brain/diagnostic imaging , Mesenchymal Stem Cell Transplantation , Mesenchymal Stem Cells/chemistry , Metal Nanoparticles/administration & dosage , Animals , Brain/drug effects , Brain/metabolism , Cobalt/chemistry , Dinoprost/analogs & derivatives , Enzyme-Linked Immunosorbent Assay , Ferric Compounds/chemistry , Iron/chemistry , Isoprostanes/analysis , Isoprostanes/metabolism , Magnetic Resonance Imaging/methods , Male , Metal Nanoparticles/chemistry , Metal Nanoparticles/toxicity , Prospective Studies , Rats, Inbred Lew , Silicon Dioxide/chemistry , Tissue Extracts , Zinc/chemistryABSTRACT
A simple, fast, sensitive and accurate methodology based on a LLE followed by liquid chromatography-tandem mass spectrometry for simultaneous determination of four regioisomers (8-iso prostaglandin F2α, 8-iso-15(R)-prostaglandin F2α, 11ß-prostaglandin F2α, 15(R)-prostaglandin F2α) in routine analysis of human plasma samples was developed. Isoprostanes are stable products of arachidonic acid peroxidation and are regarded as the most reliable markers of oxidative stress in vivo. Validation of method was performed by evaluation of the key analytical parameters such as: matrix effect, analytical curve, trueness, precision, limits of detection and limits of quantification. As a homoscedasticity was not met for analytical data, weighted linear regression was applied in order to improve the accuracy at the lower end points of calibration curve. The detection limits (LODs) ranged from 1.0 to 2.1pg/mL. For plasma samples spiked with the isoprostanes at the level of 50pg/mL, intra-and interday repeatability ranged from 2.1 to 3.5% and 0.1 to 5.1%, respectively. The applicability of the proposed approach has been verified by monitoring of isoprostane isomers level in plasma samples collected from young patients (n=8) subjected to hyperbaric hyperoxia (100% oxygen at 280kPa(a) for 30min) in a multiplace hyperbaric chamber.
Subject(s)
Chromatography, High Pressure Liquid/methods , Isoprostanes/blood , Prostaglandins/blood , Tandem Mass Spectrometry/methods , Adult , Biomarkers/analysis , Biomarkers/blood , Humans , Hyperoxia/blood , Isomerism , Isoprostanes/analysis , Limit of Detection , Linear Models , Liquid-Liquid Extraction/methods , Oxidative Stress , Prostaglandins/analysis , Young AdultABSTRACT
An experiment was conducted to evaluate the impact of lipid source on GE and ether extract (EE) digestibility, oxidative stress, and gut integrity in nursery pigs fed diets containing 10% soybean oil (SO), choice white grease (CWG), palm oil (PO), distillers' corn oil with approximately 5% FFA (DCO-1), or distillers' corn oil with approximately 10% FFA (DCO-2). Fifty-four barrows weaned at 28 d of age were fed a common starter diet for 7 d, group fed their respective experimental diets for an additional 7 d, and then moved to metabolism crates and individually fed their respective diets for another 10 d. Following this period, a 4-d total fecal and urine collection period was used to determine apparent total tract digestibility (ATTD) of GE and EE and to determine the DE and ME content of each lipid source (11.03 ± 0.51 kg final BW). Following the last day of fecal and urine collection, pigs were given an oral dose of lactulose and mannitol and fed their respective experimental diets with urine collected for the following 12 h. A subsequent urine collection occurred for 5 h to determine thiobarbituric acid reactive substances (TBARS) and isoprostane (IsoP) concentrations. Following this urine collection, serum was obtained and analyzed for TBARS and endotoxin concentrations. Soybean oil had the greatest ( < 0.05) DE (9,388 kcal/kg) content compared with DCO-1, DCO-2, CWG, and PO (8,001, 8,052, 8,531, and 8,293 kcal/kg lipid, respectively). Energy digestibility was greatest for SO compared with the other lipid sources ( < 0.05). The ATTD of EE averaged 85.0% and varied slightly (84.4 to 85.6%) among treatments. Differences in ME content among lipids were similar to those reported for DE, with ME values for DCO-1, DCO-2, CWG, PO, and SO being 7,921, 7,955, 8,535, 8,350, and 9,408 kcal/kg lipid, respectively. Metabolizable energy as a percentage of DE did not differ among lipid sources. Pigs fed lipid diets had greater ( < 0.05) serum TBARS compared with pigs fed the control diet, but no differences were observed in urinary TBARS excretion among the lipid treatments. Urinary IsoP excretion differed among treatments ( < 0.01) but was highly variable (34.0 to 104.6 pg). However, no differences were observed among treatments for the urinary lactulose:mannitol ratio and serum endotoxin. These results indicate that DE and ME content of SO are greater than that of other lipid sources evaluated, but feeding these lipids has no effect on gut integrity while producing variable effects on oxidative stress.