Fatty Acid Incorporation in the Muscle, Oxidative Markers, Lipid Peroxidation and PPAR-α and SREBP-2 Expression of Zebrafish Fed Linseed Oil and Clove Leaf Essential Oil.

The objective of this study is to assess, in zebrafish, the effects of combining linseed oil (LO) and clove leaf essential oil (CLEO) on the incorporation of fatty acids in the muscle, oxidative markers, lipid peroxidation and expression of the PPAR-α (Peroxisome Proliferator-Activated Receptor-α) and the SREBP-2 (Sterol Regulatory Element Binding Protein-2) genes. Six diets were prepared, containing combinations of LO (3, 6 and 9%) and CLEO (0.5 and 1%): 3% LO + 0.5% CLEO; 3% LO + 1% CLEO; 6% LO + 0.5% CLEO; 6% LO + 1% CLEO; 9% LO + 0.5% CLEO; 9% LO + 1% CLEO. Results showed increase in the incorporation of n-3 fatty acids in the muscle concomitantly with the addition of LO and CLEO. The activities of superoxide dismutase and catalase were reduced and the glutathione content had increased. Lipid peroxidation was lower in the treatment with 1% CLEO, regardless of LO content. The expression of the PPAR-α and the SREBP-2 genes was higher in animals fed 9% LO + 0.5% CLEO. Therefore, for a greater incorporation and protection against the oxidative damages of n-3 fatty acids, a combined use of 9% LO with 0.5% CLEO is recommended for zebrafish.

Flaxseed oil and clove leaf essential oil in Zebrafish diet (Danio rerio)

Flaxseed oil is recognized as the plant source richest in α-linolenic acid, whereas clove leaf essential oil has a strong antioxidant capacity.The objective of this study was to determine the in vitro antioxidant capacity of diets containing a combination of flaxseed oil (FO) and clove leaf essential oil (CLEO), as well as to use zebrafish (Danio rerio) to assess theireffect on the animals’growth. Fifty days after hatching, a total of 420 male specimens (0.29 ± 0.04 g)weredivided into seven groups for each diet and fed for 55 daysto be used, with the diets being: control, absent FO and CLEO; 3% FO + 0.5% CLEO; 3% FO + 1% CLEO; 6% FO + 0.5% CLEO; 6% FO + 1% CLEO; 9% FO + 0.5% CLEO and 9% FO + 1% CLEO. Antioxidant activity was determined through DPPH (2,2-diphenyl-1-picrylhydrazyl) tests, showing interaction effect between factors (FO x CLEO, p 0.05), only isolated effect for FO, with the fish fed 6 and 9% diets having the best results. Final total length and specific growth rates showed interaction effect (p <0.05). As for specific growth rates, the best response was that of the diet with 6% FO and 0.5% CLEO. Final length showed increase with FO levels, even when there was association with0.5 or 1% of CLEO. Therefore, combined use of9% of FL with 0.5% of CLEO is recommended for zebrafish.

Flaxseed oil and clove leaf essential oil in Zebrafish diet (Danio rerio)

Flaxseed oil is recognized as the plant source richest in α-linolenic acid, whereas clove leaf essential oil has a strong antioxidant capacity.The objective of this study was to determine the in vitro antioxidant capacity of diets containing a combination of flaxseed oil (FO) and clove leaf essential oil (CLEO), as well as to use zebrafish (Danio rerio) to assess theireffect on the animalsgrowth. Fifty days after hatching, a total of 420 male specimens (0.29 ± 0.04 g)weredivided into seven groups for each diet and fed for 55 daysto be used, with the diets being: control, absent FO and CLEO; 3% FO + 0.5% CLEO; 3% FO + 1% CLEO; 6% FO + 0.5% CLEO; 6% FO + 1% CLEO; 9% FO + 0.5% CLEO and 9% FO + 1% CLEO. Antioxidant activity was determined through DPPH (2,2-diphenyl-1-picrylhydrazyl) tests, showing interaction effect between factors (FO x CLEO, p <0.05); the diets containing 1% combined with 3, 6 or 9%of FOpresented meanshigher than those of the0.5%diets. No mortality was observed during the experiment. For final weight and weight gain, there was no interaction effect (p> 0.05), only isolated effect for FO, with the fish fed 6 and 9% diets having the best results. Final total length and specific growth rates showed interaction effect (p <0.05). As for specific growth rates, the best response was that of the diet with 6% FO and 0.5% CLEO. Final length showed increase with FO levels, even when there was association with0.5 or 1% of CLEO. Therefore, combined use of9% of FL with 0.5% of CLEO is recommended for zebrafish.(AU)

The stabilizing effect of cellulose crystals in O/W emulsions obtained by ultrasound process.

The encapsulation of lipophilic bioactive compounds, such as flaxseed oil, is usually done using O/W emulsions as carrier matrix. The aim of this study was to understand the stabilization mechanism of micro-nano cellulose crystals produced from acid hydrolysis in O/W emulsion. Effects of emulsification process conditions using ultrasound on the cellulose particles properties were evaluated varying the proportion of oil-cellulose particles in the emulsion formulation. Cellulose structure did not change using different conditions of emulsification and X-ray diffraction showed major presence of cellulose I. Particle size distribution of cellulose was bimodal and mean particle size reduced after hydrolysis. Emulsions stabilized by cellulose were opaque, homogeneous and showed good kinetic stability. The largest microcrystals were displayed between the oil droplets, preventing the flocculation of the droplets while smaller particles were adsorbed on the oil-water interface. The mechanism of droplets stabilization was not associated to the reduction of interfacial tension. Stabilization was associated to significant effect of electrostatic repulsion and increase in viscosity. Moreover, the flaxseed oil droplets were completely surrounded by cellulose nanocrystals, showing also Pickering-type stabilization. Therefore, emulsions with cellulose crystals were stabilized by different mechanisms and have interesting properties and characteristics for the protection of lipophilic compounds that could be applied in food and cosmetics products.

Protective Effect of α-Linolenic Acid on Non-Alcoholic Hepatic Steatosis and Interleukin-6 and -10 in Wistar Rats.

Consumption of omega-3 (n-3) polyunsaturated fatty acids (PUFA) is related to improvement in the inflammatory response associated with decreases in metabolic disorders of obesity, such as low-grade inflammation and hepatic steatosis. Linseed (Linum usitatissimum) oil is a primary source of n-3 fatty acids (FAs) of plant origin, particularly α-linolenic acid, and provides an alternative for the ingestion of n-3 PUFA by persons allergic to, or wishing to avoid, animal sources. In our study, we evaluated the effect of the consumption of different lipidic sources on metabolic and inflammatory parameters in Wistar rats. We split 56 male rats into four groups that were fed for 60 days with the following diets: sesame oil, (SO, Sesamum indicum), linseed oil (LO), SO + LO (SLO), and a control group (CG) fed with animal fat. Our results reveal that the use of LO or SLO produced improvements in the hepatic tissue, such as lower values of aspartate aminotransferase, liver weight, and hepatic steatosis. LO and SLO reduced the weight of visceral fats, weight gain, and mediated the inflammation through a decrease in interleukin (IL)-6 and increase in IL-10. Though we did not detect any significant differences in the intestine histology and the purinergic system enzymes, the consumption of α-linolenic acid appears to contribute to the inflammatory and hepatic modulation of animals compared with a diet rich in saturated FAs and or unbalanced in n-6/n-3 PUFAs, inferring possible use in treatment of metabolic disorders associated with obesity and cardiovascular diseases.

Hydrogelled emulsion from chia and linseed oils: A promising strategy to produce low-fat burgers with a healthier lipid profile.

Burgers (20% pork back fat) were produced with the replacement of 0, 20, 40, 60, 80, and 100% of pork back fat by hydrogelled emulsion (HE) from chia and linseed oils. No changes (P > .05) were observed for the moisture retention, diameter reduction, and cooking loss of the treatments, with a significant increase in the lipid retention (P < .05). Hardness increased (P < .05) with increasing the lipid replacement level, and a significant color difference (ΔE) was detected between the treatments and the control. In addition to reducing animal fat, a healthier fatty acid profile was reached after the lipid reformulation of the burgers, thus allowing the burgers to be labeled with health claims. The sensory tests (acceptance and Check-All-That-Apply) indicated that it is possible to replace up to 60% of pork back fat by HE.

Nanoencapsulation of linseed oil with chia mucilage as structuring material: Characterization, stability and enrichment of orange juice.

Linseed oil was nanoencapsulated with chia seed mucilage (CSM) as structuring material. Linseed oil nanoparticles (LO-NP) were evaluated regarding particle size distribution, zeta potential, pH, viscosity, encapsulation efficiency, loading capacity, morphology, FT-IR and thermal properties. Furthermore, the nanoparticles were spray-dried, and oxidative stability was evaluated during 28 days under storage at accelerated conditions (40 °C). The bioaccessibility of spray dried nanoparticles (SP LO-NP) was also evaluated after in vitro digestion. Thereafter, SP LO-NP were utilized in the enrichment of orange juice, and physicochemical and sensory evaluation of pure orange juice and orange juice with SP LO-NP were evaluated. Nanoparticles in suspension presented a mean diameter of 356 ±â€¯2.83 nm, zeta potential of -22.75 ±â€¯3.89 mV and encapsulation efficiency of 52%. No significant differences regarding consumer acceptance were observed between pure orange juice and orange juice with SP LO-NP. The results suggest that CSM can be used as structuring material to nanoencapsulate hydrophobic compounds, allowing its solubility in foods with high water content. Furthermore, the SP LO-NP provided a good bioaccessibility to linseed oil after in vitro digestion, which represents an advantage to incorporate the nanoparticles in food.

Unsaturated fatty acids from flaxseed oil and exercise modulate GPR120 but not GPR40 in the liver of obese mice: a new anti-inflammatory approach.

GPR120 and GPR40 were recently reported as omega-3 (ω3) receptors with anti-inflammatory properties. Physical exercise could increase the expression of these receptors in the liver, improving hepatic metabolism in obesity and type 2 diabetes. Our aim was to investigate GPR120/40 in the liver of lean and obese mice after acute or chronic physical exercise, with or without the supplementation of ω3 rich flaxseed oil (FS), as well as assess the impact of exercise and FS on insulin signaling and inflammation. Mice were fed a high-fat diet (HF) for 4 weeks to induce obesity and subsequently subjected to exercise with or without FS, or FS alone. Insulin signaling, inflammatory markers and GPR120/40 and related cascades were measured. Chronic, but not acute, exercise and FS increased GPR120, but not GPR40, activating ß-arrestin-2 and decreasing the inflammatory response, as well as reducing fat depots in liver and adipose tissue. Exercise or a source of ω3 led to a higher tolerance to fatigue and an increased running distance and speed. The combination of physical exercise and ω3 food sources could provide a new strategy against obesity through the modulation of hepatic GPR120 and an increase in exercise performance.

Omega-3 from Flaxseed Oil Protects Obese Mice Against Diabetic Retinopathy Through GPR120 Receptor.

The chronic and low-grade inflammation induced by obesity seem to be the "first hit" to retinopathy associated to diabetes type 2. Herein, we hypothesized that omega-3 fatty acids from flaxseed oil enriched diet disrupt the pro-inflammatory status in the retina, protecting against retinopathy development. For eight weeks under a high-fat diet (HF), several physiological parameters were monitored to follow the metabolic homeostasis disruption. After this period, mice were treated with a HF substituted in part of lard by flaxseed oil (FS) for another eight weeks. Food behavior, weight gain, glucose and insulin sensitivity, electroretinography, RT-qPCR and western blots were carried out. The HF was able to induce a pro-inflammatory background in the retina, changing IL1ß and TNFα. VEGF, a master piece of retinopathy, had early onset increased also induced by HF. The FS-diet was able to decrease inflammation and retinopathy and improved retinal electro stimuli compared to HF group. GPR120 and GPR40 (G Protein-Coupled Receptors 120 and 40), an omega-3 fatty acid receptors, were detected in the retina for the first time. FS-diet modulated the gene expression and protein content of these receptors. Thus, unsaturated fatty acids protect the retina from diabetes type 2 mice model from disease progression.

Flaxseed oil rich in omega-3 protects aorta against inflammation and endoplasmic reticulum stress partially mediated by GPR120 receptor in obese, diabetic and dyslipidemic mice models.

The "first hit" to atherogenesis is driven by toll-like receptor 4, endoplasmic reticulum stress and ultimately metabolic dysfunction. In this study, we hypothesized that a flaxseed oil-enriched diet (FS) abolishes these inflammatory signaling pathway and restore metabolic homeostasis by activating the fatty acid receptor GPR120 in aorta of obese mice. Glucose homeostasis was assessed by GTT and ITT; lipidomics was performed using a Hybrid Ion Trap-Orbitrap Mass Spectrometer; serum lipids were measured using colorimetric assays; GPR120 and infiltrating macrophages were analyzed by immunofluorescence; protein immunoprecipitation and gene expression were evaluated by Western blot and RT-PCR, respectively. There were no differences in body weight and food intake between the groups from both strains (Swiss and LDLr-KO mice). GTT and cholesterol levels were improved by FS in both mice models. Lipidomics showed an increase in ω3 (C18:3) content, meanwhile stearic acid (C18:0) was not detected in endothelial tissue in response to FS. Moreover, FS markedly decreased pro-inflammatory (IL-1ß, TNF-α, pIκBα, pIKKß) and unfolded protein response markers (ATF6 and GRP78) in aorta. In Swiss mice, GPR120 was partially involved in the ω3-mediated anti-inflammatory actions, disrupting TLR4 pathway, but not in LDLr-KO mice. Partial replacement of dietary saturated by unsaturated ω3 fatty acids contributes to inhibition of cardiovascular risk markers, pro-inflammatory cytokines and ER stress sensors and effectors in the aorta. However, downregulation of inflammation is not mediated by arterial GPR120 activation.

Nutritional properties and sensory analysis of yogurts made from milk of sheep fed linseed oil / Propriedades nutricionais e análise sensorial de iogurtes elaborados com leite de ovelhas alimentadas com óleo de linhaça

The objective of this study was to evaluate the effect of inclusion of linseed oil in the diets of lactating ewes on the nutritional and sensory quality of yogurts. The yogurts were produced using 3 liters of sheep milk per treatment. In the first treatment, the yogurt was made from milk of ewes that did not receive dietary addition of linseed oil during lactation, and in the second treatment the yogurt was made from milk of ewes receiving 3% linseed oil supplementation in the concentrate. The chemical composition and fatty acid profile of the milk obtained in the two treatments were analyzed. In addition, sensory analysis was performed and the purchasing intention for the yogurts was evaluated. The results showed a similar fat, protein, total solids, moisture and ash content of the two yogurts produced (P>0.05). However, a higher concentration of omega-3 fatty acids was found in yogurts made from milk of ewes that received lipid supplementation (P0.05). The addition of 3% linseed oil to the diet of lactating ewes can be recommended as an alternative feed management of dairy sheep since it provides yogurts of adequate nutritional value and sensory characteristics. In addition, the product exhibits good acceptance and purchasing intention, and appears to be a promising product for the dairy industry.(AU)

Este estudo teve por objetivo avaliar o efeito da inclusão de óleo de linhaça na dieta de ovelhas lactentes sobre a qualidade nutricional e sensorial de iogurtes. Para a fabricação dos iogurtes foram utilizando 3 litros de leite de ovelha de cada tratamento, sendo o primeiro elaborado com leite de ovelhas que não receberam durante a lactação, a adição de óleo de linhaça a dieta e o segundo, fabricado com leite obtido de ovelhas que receberam a suplementação de 3% de óleo de linhaça no concentrado da dieta. Foram avaliados a composição química e perfil de ácidos graxos do leite obtido nos dois tratamentos bem como teste sensorial, além da intenção de compra dos iogurtes. Os resultados mostraram que ambos os iogurtes desenvolvidos apresentaram teores semelhantes de gordura, proteína, sólidos totais, umidade e cinzas (P>0,05). No entanto, houve maior concentração de ácidos graxos ômega 3 nos iogurtes fabricados com leite obtidos de ovelhas que receberam suplementação lipídica (P0,05). O emprego de 3% de óleo de linhaça na dieta de ovelhas em lactação pode ser recomendado como alternativa de manejo alimentar de ovelhas leiteiras, pois proporciona a obtenção de iogurte de adequado valor nutricional e características sensoriais, bem como, boa aceitação e intenção de compra, demonstrando ser um produto promissor ao segmento lácteo.(AU)

Nutritional properties and sensory analysis of yogurts made from milk of sheep fed linseed oil / Propriedades nutricionais e análise sensorial de iogurtes elaborados com leite de ovelhas alimentadas com óleo de linhaça

The objective of this study was to evaluate the effect of inclusion of linseed oil in the diets of lactating ewes on the nutritional and sensory quality of yogurts. The yogurts were produced using 3 liters of sheep milk per treatment. In the first treatment, the yogurt was made from milk of ewes that did not receive dietary addition of linseed oil during lactation, and in the second treatment the yogurt was made from milk of ewes receiving 3% linseed oil supplementation in the concentrate. The chemical composition and fatty acid profile of the milk obtained in the two treatments were analyzed. In addition, sensory analysis was performed and the purchasing intention for the yogurts was evaluated. The results showed a similar fat, protein, total solids, moisture and ash content of the two yogurts produced (P>0.05). However, a higher concentration of omega-3 fatty acids was found in yogurts made from milk of ewes that received lipid supplementation (P0.05). The addition of 3% linseed oil to the diet of lactating ewes can be recommended as an alternative feed management of dairy sheep since it provides yogurts of adequate nutritional value and sensory characteristics. In addition, the product exhibits good acceptance and purchasing intention, and appears to be a promising product for the dairy industry.

Este estudo teve por objetivo avaliar o efeito da inclusão de óleo de linhaça na dieta de ovelhas lactentes sobre a qualidade nutricional e sensorial de iogurtes. Para a fabricação dos iogurtes foram utilizando 3 litros de leite de ovelha de cada tratamento, sendo o primeiro elaborado com leite de ovelhas que não receberam durante a lactação, a adição de óleo de linhaça a dieta e o segundo, fabricado com leite obtido de ovelhas que receberam a suplementação de 3% de óleo de linhaça no concentrado da dieta. Foram avaliados a composição química e perfil de ácidos graxos do leite obtido nos dois tratamentos bem como teste sensorial, além da intenção de compra dos iogurtes. Os resultados mostraram que ambos os iogurtes desenvolvidos apresentaram teores semelhantes de gordura, proteína, sólidos totais, umidade e cinzas (P>0,05). No entanto, houve maior concentração de ácidos graxos ômega 3 nos iogurtes fabricados com leite obtidos de ovelhas que receberam suplementação lipídica (P0,05). O emprego de 3% de óleo de linhaça na dieta de ovelhas em lactação pode ser recomendado como alternativa de manejo alimentar de ovelhas leiteiras, pois proporciona a obtenção de iogurte de adequado valor nutricional e características sensoriais, bem como, boa aceitação e intenção de compra, demonstrando ser um produto promissor ao segmento lácteo.

Oxidative stability of sesame and flaxseed oils and their effects on morphometric and biochemical parameters in an animal model.

BACKGROUND: Sesame and flaxseed oils, which are rich in essential n-6 and n-3 polyunsaturated fatty acids, are widely consumed. We have determined the optical behavior with respect to the quality and identity of cold-pressed sesame and flaxseed oils. The effects of these oils and their combinations on metabolic parameters in animal models were also measured. RESULTS: Flaxseed oil emitted carotenoid fluorescence (500-650 nm), although it was more unstable than sesame oil, which had a larger induction period by the Rancimat method. The greater stability of sesame may be a result of the lower quantity of linolenic fatty acids. These oils were added to the feed of 56 rats, whereas animal fat was used for the control group. The sesame oil, flaxseed oil and sesame + flaxseed oils groups showed a significantly reduced adiposity index and blood glucose compared to the control group, whereas total cholesterol, high-density lipoprotein and triglycerides were lower in flaxseed oil and sesame + flaxseed oils (P < 0.05). Sesame + flaxseed oils had reduced levels of low-density lipoprotein and non-high-density lipoprotein (P < 0.05), indicating an anti-atherogenic effect in this group. CONCLUSION: Sesame oil was more stable than flaxseed oil. In an animal model, the diets with polyunsaturated fat sources proportions of 1:1 n-6:n-3 polyunsaturated fatty acids, improved the metabolic parameters, implying cardioprotective effects. © 2016 Society of Chemical Industry.

High carotenoid bioaccessibility through linseed oil nanoemulsions with enhanced physical and oxidative stability.

Carotenoid (astaxanthin or lycopene) emulsions obtained by high pressure homogenization were investigated for their physical, oxidative and storage stability and biological fate on an in vitro digestion model of bioaccessibility. Emulsion stability evaluated at various processing environments (20-50°C, 2-10 pH, 0-500 mM NaCl, and 0-35 days storage at 25°C) depended on carotenoid and homogenization pressures (5, 10, 100 MPa). Trolox increased the oxidative stability of nanoemulsions (100 MPa) and acted synergistically with BHT in increasing the stability of lycopene nanoemulsion. Intestinal digestibility depended on homogenization pressures with the fastest release and lower amount of free fatty acids observed at 100 MPa. Carotenoid nanoemulsions (100 MPa) were partially (66%) digested and highly bioaccessible (>70%). Therefore, nanoemulsions provide an effective and stable system for efficient astaxanthin or lycopene delivery and bioavailability in foods, beverages, nutraceuticals and/or other agriproducts.

The chemopreventive activity of butyrate-containing structured lipids in experimental rat hepatocarcinogenesis.

SCOPE: Emerging evidence indicates that the use of bioactive food components is a promising strategy to prevent the development of liver cancer. The goal of this study was to examine the chemopreventive effect of butyrate-containing structured lipids (STLs) produced by an enzymatic interesterification of tributyrin and flaxseed oil on rat hepatocarcinogenesis. METHODS AND RESULTS: Male Wistar rats were subjected to a classic "resistant hepatocyte" model of liver carcinogenesis and treated with STLs, tributyrin or flaxseed oil during the initial phases of hepatocarcinogenesis. Treatment with STLs and tributyrin strongly inhibited the development of preneoplastic liver lesions. The chemopreventive activity of tributyrin was associated with the induction of apoptosis and reduction of the expression of major activated hepatocarcinogenesis-related oncogenes. Treatment with STLs caused substantially greater inhibitory effects than tributyrin on oncogene expression. CONCLUSION: These results demonstrate that the tumor-suppressing activity of butyrate-containing STLs is associated with its ability to prevent and inhibit activation of major hepatocarcinogenesis-related oncogenes. Enrichment of histone H3K9me3 and H3K27me3 at the promoter of Myc and Ccnd1 genes may be related to the inhibitory effect on oncogene expression in the livers of STL-treated rats.

Subcritical extraction of flaxseed oil with n-propane: Composition and purity.

Flaxseed (Linum usitatissimum L.) oil was obtained via subcritical n-propane fluid extraction (SubFE) under different temperatures and pressures with an average yield of 28% and its composition, purity and oxidative stability were compared to oils obtained via conventional solvent extraction methods (SEMs). When the oxidative stability was measured by differential scanning calorimetry, the oil was found to be up to 5 times more resistant to lipid oxidation as compared to the SEM oils. Direct infusion electrospray ionization mass spectrometry (ESI-MS) analysis showed characteristic and similar TAG profiles for SubFE and SEMs oils but higher purity for the SubFE oil. The flaxseed oil content of ß-tocopherol, campesterol, stigmasterol and sitosterol were quantified via GC-MS. SubFE showed to be a promising alternative to conventional SEM since SubFE provides an oil with higher purity and higher oxidation stability and with comparable levels of biologically active components.

Maternal exposure to diets containing flaxseed flour or flaxseed oil during pregnancy and lactation protects the aortic remodeling in adult male offspring of diabetic rat dams.

BACKGROUND: Diabetes during pregnancy is associated with cardiovascular complications in the fetus and extends into adulthood. Therapeutic applications of flaxseed have been studied in cardiovascular disorders, because its oilseed is the best plant source of omega-3 fatty acid, which is currently considered by researchers to be an essential protective against cardiovascular disease. The aim of this study was to evaluate the influence of flaxseed flour and oil on cardiovascular biochemical parameters and the histoarchitecture of the aorta in adult rats which were offspring of diabetic mothers. RESULTS: At 100 days of age in offspring it was observed that maternal consumption of a high-fat diet containing flaxseed oil (FOG) and flaxseed flour (FFG) did not affect the serum concentration of monocyte chemoattractant protein-1, vascular endothelial growth factor, cholesterol, triglycerides, high-density-, low-density- or very-low-density-lipoprotein cholesterol. However, the thickness of the intima media layer of the aorta was significantly smaller in FOG and FFG groups; the lumen area was similar among the groups; and a higher percentage of elastic fiber was found in FOG and FFG groups. CONCLUSION: These data suggest that the use of both flaxseed flour and its oil reduces the remodeling of the aorta; however; it has not been possible to modify the cardiovascular biochemical parameters.

Oxidative stability and sensory evaluation of microencapsulated flaxseed oil.

Cold pressed flaxseed oil was microencapsulated by spray drying using an emulsion containing modified starch. The fatty acid composition, moisture, water activity, wettability, water holding capacity, water solubility, crystallinity, and particle size distribution of the microcapsules were determined. The stability of the microcapsules and the crude oil were assessed. An acceptance test was used for the sensory evaluation of a powdered supplement containing the microcapsules. The fatty acid composition was not significantly affected by the microencapsulation. The moisture, water activity, wettability, water solubility and crystallinity were appropriate for dry powders. The microcapsules had no cracks and showed better oxidative stability compared with the crude oil. Storage under vacuum prevented oxidation of the microcapsules. In sensory evaluation, all quality scores of the supplement containing microcapsules were mid-range or higher. The microencapsulation improved the oxidative stability of the oil and this procedure was satisfactorily applied in powdered food.

Quality traits and lipid composition of meat from Nellore young bulls fed with different oils either protected or unprotected from rumen degradation.

The qualitative characteristics, lipids and chemical composition of the meat of 35 Nellore young bulls were analyzed. These bulls had an average slaughter weight and fat thickness of 532.17±30.2 kg, and 7.00 mm, respectively. Significant differences were found only in the meat's water holding capacity (WHC), which was higher for animals fed with fresh linseed oil. More conjugated linoleic acid (CLA) was found in the meat of animals fed with unprotected soybean oil, while better omega-6/omega-3 ratios were noted for those fed unprotected linseed oil. The addition of different vegetable oils to the bulls' diet (soybean or linseed, either protected or not protected from rumen digestion) did not interfere with the qualitative characteristics of their meat while improving the lipid composition of the longissimus muscle. Of the oils examined, unprotected linseed oil most improved the omega-6/omega-3 ratio, thus producing the healthiest meat for human consumption.

Lipid oxidation stability of omega-3- and conjugated linoleic acid-enriched sous vide chicken meat.

Lipid oxidation is known to occur rather rapidly in cooked chicken meat containing relatively high amounts of polyunsaturated fatty acids. To assess the lipid oxidation stability of sous vide chicken meat enriched with n-3 and conjugated linoleic acid (CLA) fatty acids, 624 Cobb × Ross broilers were raised during a 6-wk feeding period. The birds were fed diets containing CLA (50% cis-9, trans-11 and 50% trans-10, cis-12 isomers), flaxseed oil (FSO), or menhaden fish oil (MFO), each supplemented with 42 or 200 mg/kg of vitamin E (dl-α-tocopheryl acetate). Breast or thigh meat was vacuum-packed, cooked (74°C), cooled in ice water, and stored at 4.4°C for 0, 5, 10, 15, and 30 d. The lipid oxidation development of the meat was estimated by quantification of malonaldehyde (MDA) values, using the 2-thiobarbituric acid reactive substances analysis. Fatty acid, nonheme iron, moisture, and fat analyses were performed as well. Results showed that dietary CLA induced deposition of cis-9, trans-11 and trans-10, cis-12 CLA isomers, increased the proportion of saturated fatty acids, and decreased the proportions of monounsaturated and polyunsaturated fatty acids. Flaxseed oil induced higher deposition of C18:1, C18:2, C18:3, and C20:4 fatty acids, whereas MFO induced higher deposition of n-3 fatty acids, eicosapentaenoic acid (C20:5), and docosahexaenoic acid (C22:6; P < 0.05). Meat lipid oxidation stability was affected by the interaction of either dietary oil or vitamin E with storage day. Lower (P < 0.05) MDA values were found in the CLA treatment than in the MFO and FSO treatments. Lower (P < 0.05) MDA values were detected in meat samples from the 200 mg/kg of vitamin E than in meat samples from the 42 mg/kg of vitamin E. Nonheme iron values did not affect (P > 0.05) lipid oxidation development. In conclusion, dietary CLA, FSO, and MFO influenced the fatty acid composition of chicken muscle and the lipid oxidation stability of meat over the storage time. Supranutritional supplementation of vitamin E enhanced the lipid oxidation stability of sous vide chicken meat.