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1.
Am J Physiol Regul Integr Comp Physiol ; 320(4): R541-R546, 2021 04 01.
Article in English | MEDLINE | ID: mdl-33533311

ABSTRACT

Physical exercise attenuates the development of l-3,4-dihydroxyphenylalanine (l-DOPA)-induced dyskinesia (LID) in 6-hydroxydopamine-induced hemiparkinsonian mice through unknown mechanisms. We now tested if exercise normalizes the aberrant corticostriatal neuroplasticity associated with experimental murine models of LID. C57BL/6 mice received two unilateral intrastriatal injections of 6-hydroxydopamine (12 µg) and were treated after 3 wk with l-DOPA/benserazide (25/12.5 mg/kg) for 4 wk, with individualized moderate-intensity running (60%-70% V̇o2peak) or not (untrained). l-DOPA converted the pattern of plasticity in corticostriatal synapses from a long-term depression (LTD) into a long-term potentiation (LTP). Exercise reduced LID severity and decreased aberrant LTP. These results suggest that exercise attenuates abnormal corticostriatal plasticity to decrease LID.


Subject(s)
Antiparkinson Agents/toxicity , Cerebral Cortex/drug effects , Corpus Striatum/drug effects , Dyskinesia, Drug-Induced/prevention & control , Exercise Therapy , Levodopa/toxicity , Neuronal Plasticity/drug effects , Parkinsonian Disorders/drug therapy , Animals , Benserazide/toxicity , Cerebral Cortex/physiopathology , Corpus Striatum/physiopathology , Dihydroxyphenylalanine/analogs & derivatives , Disease Models, Animal , Dyskinesia, Drug-Induced/etiology , Dyskinesia, Drug-Induced/physiopathology , Long-Term Potentiation/drug effects , Long-Term Synaptic Depression/drug effects , Male , Mice, Inbred C57BL , Motor Activity/drug effects , Parkinsonian Disorders/chemically induced , Parkinsonian Disorders/physiopathology , Running , Time Factors
2.
Synapse ; 70(6): 240-52, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26860222

ABSTRACT

Erythropoietin has shown wide physiological effects on the central nervous system in animal models of disease, and in healthy animals. We have recently shown that systemic EPO administration 15 min, but not 5 h, after daily training in a water maze is able to induce the recovery of spatial memory in fimbria-fornix chronic-lesioned animals, suggesting that acute EPO triggers mechanisms which can modulate the active neural plasticity mechanism involved in spatial memory acquisition in lesioned animals. Additionally, this EPO effect is accompanied by the up-regulation of plasticity-related early genes. More remarkably, this time-dependent effects on learning recovery could signify that EPO in nerve system modulate specific living-cellular processes. In the present article, we focus on the question if EPO could modulate the induction of long-term synaptic plasticity like LTP and LTD, which presumably could support our previous published data. Our results show that acute EPO peripheral administration 15 min before the induction of synaptic plasticity is able to increase the magnitude of the LTP (more prominent in PSA than fEPSP-Slope) to facilitate the induction of LTD, and to protect LTP from depotentiation. These findings showing that EPO modulates in vivo synaptic plasticity sustain the assumption that EPO can act not only as a neuroprotective substance, but is also able to modulate transient neural plasticity mechanisms and therefore to promote the recovery of nerve function after an established chronic brain lesion. According to these results, EPO could be use as a molecular tool for neurorestaurative treatments.


Subject(s)
Dentate Gyrus/drug effects , Erythropoietin/pharmacology , Hippocampus/drug effects , Long-Term Potentiation/drug effects , Neuronal Plasticity/drug effects , Synapses/drug effects , Synaptic Transmission/drug effects , Animals , Long-Term Synaptic Depression/drug effects , Male , Memory/drug effects , Neuronal Plasticity/physiology , Rats, Wistar , Synaptic Transmission/physiology , Up-Regulation
3.
Neuropharmacology ; 65: 143-55, 2013 Feb.
Article in English | MEDLINE | ID: mdl-23022398

ABSTRACT

Cholinergic fibers from the brainstem and basal forebrain innervate the medial prefrontal cortex (mPFC) modulating neuronal activity and synaptic plasticity responses to hippocampal inputs. Here, we investigated the muscarinic and glutamatergic modulation of long-term depression (LTD) in the intact projections from CA1 to mPFC in vivo. Cortical-evoked responses were recorded in urethane-anesthetized rats for 30 min during baseline and 4 h following LTD. In order to test the potentiating effects of pilocarpine (PILO), independent groups of rats received either a microinjection of PILO (40 nmol; i.c.v.) or vehicle, immediately before or 20 min after a sub-threshold LTD protocol (600 pulses, 1 Hz; LFS600). Other groups received either an infusion of the selective NMDA receptor antagonist (AP7; 10 nmol; intra-mPFC) or vehicle, 10 min prior to PILO preceding LFS600, or prior to a supra-threshold LTD protocol (900 pulses, 1 Hz; LFS900). Our results show that PILO converts a transient cortical depression induced by LFS600 into a robust LTD, stable for at least 4 h. When applied after LFS600, PILO does not change either mPFC basal neurotransmission or late LTD. Our data also indicate that NMDA receptor pre-activation is essential to the muscarinic enhancement of mPFC synaptic depression, since AP7 microinjection into the mPFC blocked the conversion of transient depression into long-lasting LTD produced by PILO. In addition, AP7 effectively blocked the long-lasting LTD induced by LFS900. Therefore, our findings suggest that the glutamatergic co-activation of prefrontal neurons is important for the effects of PILO on mPFC synaptic depression, which could play an important role in the control of executive and emotional functions.


Subject(s)
Brain Waves/physiology , Hippocampus/physiology , Long-Term Synaptic Depression/physiology , Muscarinic Agonists/administration & dosage , Prefrontal Cortex/physiology , Receptors, N-Methyl-D-Aspartate/physiology , Animals , Brain Waves/drug effects , Excitatory Amino Acid Antagonists/administration & dosage , Hippocampus/drug effects , Long-Term Synaptic Depression/drug effects , Male , Microinjections , Neural Pathways/drug effects , Neural Pathways/physiology , Neuronal Plasticity/drug effects , Neuronal Plasticity/physiology , Pilocarpine/administration & dosage , Prefrontal Cortex/drug effects , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Time Factors
4.
PLoS One ; 7(10): e47484, 2012.
Article in English | MEDLINE | ID: mdl-23118873

ABSTRACT

The mediodorsal nucleus of the thalamus (MD) is a rich source of afferents to the medial prefrontal cortex (mPFC). Dysfunctions in the thalamo-prefrontal connections can impair networks implicated in working memory, some of which are affected in Alzheimer disease and schizophrenia. Considering the importance of the cholinergic system to cortical functioning, our study aimed to investigate the effects of global cholinergic activation of the brain on MD-mPFC synaptic plasticity by measuring the dynamics of long-term potentiation (LTP) and depression (LTD) in vivo. Therefore, rats received intraventricular injections either of the muscarinic agonist pilocarpine (PILO; 40 nmol/µL), the nicotinic agonist nicotine (NIC; 320 nmol/µL), or vehicle. The injections were administered prior to either thalamic high-frequency (HFS) or low-frequency stimulation (LFS). Test pulses were applied to MD for 30 min during baseline and 240 min after HFS or LFS, while field postsynaptic potentials were recorded in the mPFC. The transient oscillatory effects of PILO and NIC were monitored through recording of thalamic and cortical local field potentials. Our results show that HFS did not affect mPFC responses in vehicle-injected rats, but induced a delayed-onset LTP with distinct effects when applied following PILO or NIC. Conversely, LFS induced a stable LTD in control subjects, but was unable to induce LTD when applied after PILO or NIC. Taken together, our findings show distinct modulatory effects of each cholinergic brain activation on MD-mPFC plasticity following HFS and LFS. The LTP-inducing action and long-lasting suppression of cortical LTD induced by PILO and NIC might implicate differential modulation of thalamo-prefrontal functions under low and high input drive.


Subject(s)
Muscarinic Agonists/administration & dosage , Neuronal Plasticity , Prefrontal Cortex , Synapses , Thalamus , Animals , Electric Stimulation , Long-Term Potentiation/drug effects , Long-Term Potentiation/physiology , Long-Term Synaptic Depression/drug effects , Long-Term Synaptic Depression/physiology , Male , Memory, Short-Term/drug effects , Neuronal Plasticity/drug effects , Neuronal Plasticity/physiology , Nicotine/administration & dosage , Pilocarpine/administration & dosage , Prefrontal Cortex/drug effects , Prefrontal Cortex/metabolism , Rats , Synapses/drug effects , Synapses/physiology , Thalamus/drug effects
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