ABSTRACT
For the first time, we suggest using leaf extract from Ocimum americanum as the economically viable bio-fabrication of copper nanomaterials. The residuals of leaf extract bio-capping provide the stability of the nanomaterials in-situ. UV-Vis and XRD confirmed the formation, with the UV-Vis spectrum of Cu-NMs revealing a surface plasmon resonance characteristic peak at 350 nm. FT-IR analysis was employed to examine the functional groups. FE-SEM with EDX was used to assess the morphology and carry out an elemental analysis of the nanomaterials. Diffusion and MTT assays were used to study the antimicrobial and anticancer activities. The synthesized copper nanomaterials exhibited in-vitro cytotoxicity against human skin cancer (A431) cell lines. Green nanomaterial was examined against the methylene blue dye, photodegradation was reduced by up to 90.6% within 50 minutes. The copper nanomaterials synthesized in our study exhibit promising applications in biomedicine and environmental pollution research.
Subject(s)
Cell Proliferation , Copper , Copper/chemistry , Copper/pharmacology , Humans , Cell Proliferation/drug effects , Cell Line, Tumor , Nanostructures/chemistry , Green Chemistry Technology , Antineoplastic Agents/pharmacology , Antineoplastic Agents/chemistry , Metal Nanoparticles/chemistry , Luminescence , Plant Extracts/chemistry , Plant Extracts/pharmacology , Drug Screening Assays, Antitumor , Plant Leaves/chemistry , Anti-Bacterial Agents/pharmacology , Anti-Bacterial Agents/chemistry , Particle Size , Microbial Sensitivity Tests , Luminescent Agents/chemistry , Luminescent Agents/pharmacology , Luminescent Agents/chemical synthesisABSTRACT
Organometallic compounds are increasingly recognized as promising anticancer and antibiotic drug candidates. Among the transition metal ions investigated for these purposes, rhenium occupies a special role. Its tri- and dicarbonyl complexes, in particular, attract continuous attention due to their relative ease of preparation, stability and unique photophysical and luminescent properties that allow the combination of diagnostic and therapeutic purposes, thereby permitting, e.g., molecules to be tracked within cells. In this review, we discuss the anticancer and antibiotic properties of rhenium tri- and dicarbonyl complexes described in the last seven years, mainly in terms of their structural variations and in vitro efficacy. Given the abundant literature available, the focus is initially directed on tricarbonyl complexes of rhenium. Dicarbonyl species of the metal ion, which are slowly gaining momentum, are discussed in the second part in terms of future perspective for the possible developments in the field.
Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , Rhenium/chemistry , Rhenium/pharmacology , Animals , Anti-Bacterial Agents/therapeutic use , Antineoplastic Agents/therapeutic use , Humans , Luminescent Agents/chemistry , Luminescent Agents/pharmacology , Luminescent Agents/therapeutic use , Organometallic Compounds/chemistry , Organometallic Compounds/pharmacology , Organometallic Compounds/therapeutic use , Rhenium/therapeutic useABSTRACT
Trivalent europium-based monochromatic red light-emitting phosphors are an essential component to realize high-performance smart lighting devices; however, the concentration and thermal quenching restrict their usage. Here, we report a series of efficient Eu3+-substituted Li3Y3BaSr(MoO4)8 red-emitting phosphors based on a stratified scheelite structure with negligible concentration and thermal quenching. All of the host and phosphor compositions crystallize in monoclinic crystal structure (space group C2/c). All of the phosphor compositions produce narrow-band red emission (FWHM â¼6 nm), which is highly apparent to the human eyes, and lead to exceptional chromatic saturation of the red spectral window. Concurrently, detailed investigations were carried out to comprehend the concentration and thermal quenching mechanism. Absolute quantum yields as high as 88.5% were obtained for Li3Y0.3Eu2.7BaSr(MoO4)8 phosphor with virtuous thermal stability (at 400 K, retaining 87% of its emission intensity). The light-emitting diodes were constructed by coupling Li3BaSrY0.3Eu2.7(MoO4)8 red phosphor with a near-UV LED chip (395 nm) operated at 20 mA forward bias, and the hybrid white LED (an organic yellow dye + red Li3Y3BaSr(MoO4)8:Eu3+ phosphor integrated with an NUV LED chip) showed a low CCT (6645 K), high CRI (83) values, and CIE values of x = 0.303; y = 0.368, which indicated that the synthesized phosphors can be a suitable red component for white LEDs. In addition, we have systematically investigated the Sm3+ and Sm3+, Eu3+ activation in Li3Y3BaSr(MoO4)8 to display the latent use of the system in plant growth applications and establish that the phosphor exhibits orange red emission with an intense deep-red emission (645 nm (4G5/2 â 6H9/2)). The phytochrome (Pr) absorption spectrum well matched the fabricated deep-red LED (by integrating a NUV LED + Li3Y3BaSr(MoO4)8:Sm3+ and Eu3+ phosphor) spectral lines.
Subject(s)
Color , Light , Luminescent Agents/pharmacology , Plants/drug effects , Barium/chemistry , Barium/pharmacology , Europium/chemistry , Europium/pharmacology , Humans , Lithium/chemistry , Lithium/pharmacology , Luminescent Agents/chemistry , Luminescent Measurements , Molybdenum/chemistry , Molybdenum/pharmacology , Phosphorus/chemistry , Phosphorus/pharmacology , Samarium/chemistry , Samarium/pharmacology , Strontium/chemistry , Strontium/pharmacology , TemperatureABSTRACT
The encapsulation and/or surface modification can stabilize and protect the phosphorescence bio-probes but impede their intravenous delivery across biological barriers. Here, a new class of biocompatible rhenium (ReI ) diimine carbonyl complexes is developed, which can efficaciously permeate normal vessel walls and then functionalize the extravascular collagen matrixes as in situ oxygen sensor. Without protective agents, ReI -diimine complex already exhibits excellent emission yield (34%, λem = 583 nm) and large two-photon absorption cross-sections (σ2 = 300 GM @ 800 nm) in water (pH 7.4). After extravasation, remarkably, the collagen-bound probes further enhanced their excitation efficiency by increasing the deoxygenated lifetime from 4.0 to 7.5 µs, paving a way to visualize tumor hypoxia and tissue ischemia in vivo. The post-extravasation functionalization of extracellular matrixes demonstrates a new methodology for biomaterial-empowered phosphorescence sensing and imaging.
Subject(s)
Blood Vessels/diagnostic imaging , Collagen/metabolism , Luminescent Agents/pharmacology , Oxygen/metabolism , Blood Vessels/drug effects , Blood Vessels/metabolism , Blood Vessels/pathology , Collagen/genetics , Humans , Iridium/pharmacology , Microscopy, Confocal , Neoplasms/genetics , Neoplasms/pathology , Photons , Rhenium/chemistry , Tumor Hypoxia/genetics , Tumor Microenvironment/drug effects , Tumor Microenvironment/geneticsABSTRACT
The role of small molecules in the preparation of metal nanomaterials generates considerable interest in the fields from materials science to interdisciplinary sciences. In this study, a small amino acid, l-tyrosine (Tyr), has been used as a ligand precursor for the preparation of silver nanomaterials (AgNMs) comprising a dual system: smaller silver nanoclusters (responsible exclusively for the photophysical properties) and larger silver nanoparticles (responsible exclusively for the antimicrobial properties). The luminescent properties of this AgNM system substantiate the role played by Tyr as a capping and a reducing agent outside the protein environment. An interesting feature of this report is the promising antimicrobial properties of the AgNMs against Saccharomyces cerevisiae, Candida albicans, Escherichia coli, and Bacillus cereus cell lines. The importance of this work is that this investigation demonstrates the combating ability of our AgNM system against pathogenic strains (C. albicans and B. cereus) as well. Moreover, the mechanistic aspects of the antimicrobial activity of the AgNMs were elucidated using various methods, such as propidium iodide staining, monitoring reactive oxygen species generation, leakage of proteins, DNA cleavage, etc. We propose that AgNM-mediated cytotoxicity in S. cerevisiae stems from the generation of singlet oxygen (1O2) species that create oxidative stress, disrupting the cell membrane and thereby resulting in leakage of proteins from the cells. This study can pave the way toward elucidating the role of a small molecule, Tyr, in the formation of NMs and describes the use of new NMs in potential antimicrobial applications.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Luminescent Agents/pharmacology , Metal Nanoparticles/chemistry , Silver/pharmacology , Singlet Oxygen/metabolism , Anti-Bacterial Agents/chemical synthesis , Antifungal Agents/chemical synthesis , Bacillus cereus/drug effects , Candida albicans/drug effects , Escherichia coli/drug effects , Luminescent Agents/chemical synthesis , Microbial Sensitivity Tests , Saccharomyces cerevisiae/drug effects , Silver/chemistry , Tyrosine/chemistryABSTRACT
X-ray excited persistent luminescence (XEPL) imaging has attracted increasing attention in biomedical imaging due to elimination of autofluorescence, high signal-to-noise ratio and repeatable activation with high penetration. However, optical imaging still suffers from limited for high spatial resolution. Methods: Herein, we report Mn3+-rich manganese oxide (MnOx)-coated chromium-doped zinc gallogermanate (ZGGO) nanoparticles (Mn-ZGGOs). Enhanced XEPL and magnetic resonance (MR) imaging were investigated by the decomposition of MnOx shell in the environment of tumors. We also evaluated the tumor cell-killing mechanism by detection of reactive oxygen (ROS), lipid peroxidation and mitochondrial membrane potential changes in vitro. Furthermore, the in vivo biodistribution, imaging and therapy were studied by U87MG tumor-bearing mice. Results: In the tumor region, the MnOx shell is quickly decomposed to produce Mn3+ and oxygen (O2) to directly generate singlet oxygen (1O2). The resulting Mn2+ transforms endogenous H2O2 into highly toxic hydroxyl radical (·OH) via a Fenton-like reaction. The Mn2+ ions and ZGGOs also exhibit excellent T1-weighted magnetic resonance (MR) imaging and ultrasensitive XEPL imaging in tumors. Conclusion: Both the responsive dual-mode imaging and simultaneous self-supplied O2 for the production of 1O2 and oxygen-independent ·OH in tumors allow for more accurate diagnosis of deep tumors and more efficient inhibition of tumor growth without external activation energy.
Subject(s)
Hydroxyl Radical/metabolism , Luminescent Agents , Manganese Compounds , Nanoparticles , Neoplasms, Experimental , Optical Imaging , Oxides , Singlet Oxygen/metabolism , Animals , Cell Line, Tumor , Humans , Luminescent Agents/chemistry , Luminescent Agents/pharmacokinetics , Luminescent Agents/pharmacology , Manganese Compounds/chemistry , Manganese Compounds/pharmacokinetics , Manganese Compounds/pharmacology , Mice , Nanoparticles/chemistry , Nanoparticles/therapeutic use , Neoplasms, Experimental/diagnostic imaging , Neoplasms, Experimental/drug therapy , Neoplasms, Experimental/metabolism , Oxides/chemistry , Oxides/pharmacokinetics , Oxides/pharmacology , Xenograft Model Antitumor AssaysABSTRACT
A facile chemical route is reported for synthesizing red-emitting photoluminescent/MRI multi-functional KLa(0.95-x)GdxF4:Eu3+ (x = 0 to 0.4) bio-compatible nanomaterials for targeted in vitro tumor imaging. Hexagonal phase pure nanoparticles show a significant and systematic change in morphology with enhanced photoluminescence due to the substitution of La3+ with Gd3+ ions. Single phase ß-KLa(0.95-x)GdxF4:Eu3+ exhibits multifunctional properties, both intense red emission and strong paramagnetism for high-contrast bioimaging applications. These silica capped magnetic/luminescent nanoparticles show long-term colloidal stability, optical transparency in water, strong red emission, and low cytotoxicity. The cellular uptake of coated nanoparticles was investigated in liver cancer cell line Huh-7. Our findings suggest that these nanoparticles can serve as highly luminescent imaging probes for in vitro applications with potential for in vivo and live cell imaging applications.
Subject(s)
Antineoplastic Agents/chemistry , Liver Neoplasms/diagnostic imaging , Luminescent Agents/chemistry , Nanoparticles/chemistry , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Cell Proliferation/drug effects , Cell Survival/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Europium/chemistry , Fluorine/chemistry , Gadolinium/chemistry , Humans , Lanthanoid Series Elements/chemistry , Luminescent Agents/chemical synthesis , Luminescent Agents/pharmacology , Particle Size , Potassium/chemistry , Tumor Cells, CulturedABSTRACT
Narrow-band red-emitters are the key to solving problems encountered by the current white LED technology. In this context, a series of new red-emitting Li3BaSrLa3(MoO4)8:Eu3+ phosphors were synthesized and characterized through various spectroscopic methods. All phosphor compositions were crystallized in the monoclinic phase, with space group C2/c. A broad charge transfer (O2-â Mo6+) extended up to the blue region along with strong 7F0â5L6, 5D3 absorption, making them looked-for materials for warm white LED applications. The concentration quenching study reveals that there was no concentration-quenching occuring and the quantum yield of this non-concentration-quenching Li3BaSrLa0.3Eu2.7(MoO4)8 phosphor reaches 92.6%. The Li3BaSrLa0.3Eu2.7(MoO4)8 retain >80% of its emission intensity at 150 °C. The best red-emitting composition was integrated with near UV LED and obtained bright red emission with CIE x = 0.6647, y = 0.3357. White LED was fabricated by integrating the blue LED with yellow dye + red phosphor and white LED showed bright white light with CCT (5546 K), CIE (0.331, 0.385), and CRI (81%). In addition, the red LED spectrum is well-matched with the phytochrome (Pr) absorption spectrum and is useful for plant growth applications.
Subject(s)
Luminescent Agents/pharmacology , Plant Development/drug effects , Crystallography, X-Ray , Luminescent Agents/chemical synthesis , Luminescent Agents/chemistry , Models, Molecular , Particle SizeABSTRACT
Four new cyclometalated Pt(ii) complexes bearing acyclic diaminocarbene (ADC) ligands, [Pt(C^N)Cl{C(NHXyl)(NHR)}] [C^N = 2,6-difluorophenylpyridine (dfppy), phenylquinoline (pq); R = Pr 3a, 4a, CH2Ph 3b, 4b], were prepared by the nucleophilic attack on the isocyanide [Pt(C^N)Cl(CNXyl)] (C^N = dfppy 1, pq 2) by the corresponding amine RNH2 (R = Pr, CH2Ph). Complexes 3 show in their 1H NMR spectra in CDCl3 a notable concentration dependence, with a clear variation of the δH (NHXyl) signal, suggesting an assembling process implying donor-acceptor NHXylCl bonding, also supported by 1D-PGSE (Pulse Field Gradient Spin Echo) and 2D-DOSY (Diffusion Ordered Spectroscopy) NMR experiments in solution and X-ray diffraction studies. The intermolecular interactions in compounds 3a and 3b were studied by using Hirshfeld surface analysis and Non-Covalent Interaction (NCI) methods on their X-ray structures. Their photophysical properties were investigated by absorption and emission spectroscopies and also by TD-DFT calculations performed on 3a and 4b. These complexes show green (3) or orange (4) phosphorescence, attributed to a mixed 3IL/3MLCT excited state. The carbene ligand does not affect the emission maxima but it produces an increase of the quantum yields in relation to the isocyanide in the precursors. In fluid solutions, the emission is not concentration-dependent, but the complexes may show aggregation induced emission as detailed for complexes 3a and 4a. In addition, cytotoxicity studies in the human cell lines A549 (lung carcinoma) and HeLa (cervix carcinoma) showed good activity for these complexes and 3a, 3b and 4a exhibit a strong effect on DNA electrophoretic mobility. To the best of our knowledge, compounds 3 and 4 represent the first examples of cycloplatinated complexes bearing acyclic diamino carbenes with antiproliferative properties.
Subject(s)
Alkynes/pharmacology , Antineoplastic Agents/pharmacology , Dioxolanes/pharmacology , Luminescent Agents/pharmacology , Organoplatinum Compounds/pharmacology , Alkynes/chemistry , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Cell Proliferation/drug effects , Cell Survival/drug effects , Crystallography, X-Ray , Density Functional Theory , Dioxolanes/chemistry , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , Humans , Ligands , Luminescent Agents/chemical synthesis , Luminescent Agents/chemistry , Models, Molecular , Molecular Structure , Organoplatinum Compounds/chemical synthesis , Organoplatinum Compounds/chemistry , Structure-Activity Relationship , Tumor Cells, CulturedABSTRACT
The mitotic kinesin Eg5 is a plus-end directed homotetrameric molecular motor essential for the formation of bipolar spindles during cell division. Kinesin Eg5 is overexpressed in cancer cells and hence considered as a target for cancer therapy; the inhibitors specific for Eg5 have been developed as anticancer drugs. In this study, we synthesized a novel functional photoresponsive inhibitor composed of spiropyran and azobenzene derivatives to control Eg5 function with multistage inhibitory activity accompanied by the formation of different isomerization states. The photochromic inhibitor spiropyran-sulfo-azobenzene (SPSAB) exhibited three isomerization states: spiro (SP)-trans, merocyanine (MC)-cis and MC-trans, upon exposure to visible light, ultraviolet and in the dark, respectively. SPSAB-induced reversible changes in the inhibitory activity of ATPase and motor activities correlating with photoisomerization among the three states. Among the three isomerization states of SPSAB, the SP-trans isomer showed potent inhibitory activity at an IC50 value of 30 µM in the basal ATPase assay. MC-trans and MC-cis exhibited less inhibitory activity at IC50 values of 38 and 86 µM, respectively. The results demonstrated that the novel photochromic inhibitor enabled precise control of Eg5 function at three different levels using light irradiation.
Subject(s)
Azo Compounds/pharmacology , Benzopyrans/pharmacology , Indoles/pharmacology , Kinesins/antagonists & inhibitors , Kinesins/metabolism , Mitosis , Nitro Compounds/pharmacology , Adenosine Triphosphatases/metabolism , Azo Compounds/chemistry , Azo Compounds/metabolism , Benzopyrans/chemistry , Benzopyrans/metabolism , Cell Division/drug effects , Cell Movement/drug effects , Humans , Indoles/chemistry , Indoles/metabolism , Isomerism , Kinesins/chemistry , Light , Luminescent Agents/pharmacology , Microtubules/metabolism , Nitro Compounds/chemistry , Nitro Compounds/metabolism , Protein BindingABSTRACT
We designed a biodegradable hybrid nanostructure for near-infrared (NIR)-induced photodynamic therapy (PDT) using an ultrasmall upconversion (UC) phosphor (ß-NaYF4:Yb3+, Er3+ nanoparticle: NPs) and a hydrocarbonized rose bengal (C18RB) dye, a hydrophobized rose bengal (RB) derivative. The UC-NPs were encapsulated along with C18RB in the hydrophobic core of the micelle composed of poly(ethylene glycol) (PEG)-block-poly(ε-caprolactone) (PCL). The UC-NPs were well shielded from the aqueous environment, owing to the encapsulation in the hydrophobic PCL core, to efficiently emit green UC luminescence by avoiding the quenching by the hydroxyl groups. The hydrophobic part of C18 of C18RB worked well to be involved in the PCL core and located RB on the surface of the PCL core, making the efficient absorption of green light and the emission of singlet oxygen to surrounding water possible. Moreover, as the location is covered by PEG, the direct contact of RB to cells is prohibited to avoid their irradiation-free toxic effect on the cells. The hybrid nanostructure proved to be degradable by the hydrolysis of PEG-b-PCL. This degradation potentially results in renal excretion by the decomposition of the nanostructure into sub-10 nm size particles and makes them viable for clinical uses. These nanostructures can potentially be used for PDT of cancer in deep tissues.
Subject(s)
Antineoplastic Agents/pharmacology , Biocompatible Materials/pharmacology , Luminescent Agents/pharmacology , Nanostructures/chemistry , Photochemotherapy , Photosensitizing Agents/pharmacology , Rose Bengal/chemistry , Animals , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Biocompatible Materials/chemical synthesis , Biocompatible Materials/chemistry , Cell Line, Tumor , Cell Survival/drug effects , Drug Screening Assays, Antitumor , Infrared Rays , Luminescent Agents/chemical synthesis , Luminescent Agents/chemistry , Materials Testing , Particle Size , Photosensitizing Agents/chemical synthesis , Photosensitizing Agents/chemistryABSTRACT
Nanoscale materials have been explored as better alternatives to conventional therapeutic agents in cancer theranostics in the recent period due to efficacy in overcoming biological, biomedical, and biophysical barriers. Analysis on the ability of copper nanocluster (CuNC)-doped hydroxyapatite nanoparticles (Cu-HXNPs) as suitable nanocarriers for anticell proliferative application was carried out. Having high adsorption capacity, the Cu-HXNPs could be loaded with the anticancer drug quercetin, which is a polyphenolic flavonoid compound, and were used as nanocarriers to be applied on HeLa (cancer cells) and HEK-293 (normal cells). The drug release profile was found to be pH-dependent, where maximum release of quercetin from quercetin-loaded Cu-HXNPs was observed in acidic pH as compared to physiological pH. The Cu-HXNPs could release quercetin, which could effectively decline proliferation of cancer cells via generation of reactive oxygen species. Moreover, the released quercetin significantly altered the cell cycle pattern and triggered the cells to undergo apoptosis. Additionally, the efficacy of Cu-HXNPs as a nanocarrier to release quercetin on 3D spheroids of HeLa had been checked, which demonstrated significant reduction in the viability of 3D spheroids. The luminescent CuNCs used for doping HXNPs endowed the nanocarrier with the imaging property, which was an excellent feature in confirming their uptake by the cells. Thus, the study suggested Cu-HXNPs to be a beneficial nanocarrier for both bioimaging and therapeutic purpose in the field of cancer theranostics.
Subject(s)
Antineoplastic Agents/pharmacology , Biocompatible Materials/pharmacology , Durapatite/pharmacology , Luminescent Agents/pharmacology , Quercetin/pharmacology , Theranostic Nanomedicine , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Apoptosis/drug effects , Biocompatible Materials/chemical synthesis , Biocompatible Materials/chemistry , Cell Line , Cell Proliferation/drug effects , Drug Screening Assays, Antitumor , Durapatite/chemistry , Humans , Hydrogen-Ion Concentration , Luminescent Agents/chemistry , Materials Testing , Molecular Structure , Nanoparticles/chemistry , Particle Size , Quercetin/chemistryABSTRACT
Due to several negative issues, market available drugs have been gradually losing their importance in the treatment of cancer. With a view to discover suitable drugs capable of diagnosing as well as inhibiting the growth of cancer cells, we have aspired to develop a group of theranostic metal complexes which will be (i) target specific, (ii) cytoselective, thus rendering the normal cell unaffected, (iii) water-soluble, (iv) cancer cell permeable, and (v) luminescent, being beneficial for healing the cancer eternally. Therefore, to reach our goal, we have prepared novel Ru(II)- and Ir(III)-based bimetallic and hetero bimetallic scaffolds using click-derived pyridinyltriazolylmethylquinoxaline ligands followed by metal coordination. Most of the compounds have displayed significant cytoselectivity against colorectal adenocarcinoma (Caco-2) and epithiloid cervical carcinoma (HeLa) cells with respect to normal human embryonic kidney cells (HEK-293) compared to cisplatin [cis-diamminedichloroplatinum(II)] along with excellent binding efficacy with DNA as well as serum albumin. Complex [(η6-p-cymene)(η5-Cp*)RuIIIrIIICl2(K2-N,N-L)](PF6)2 [RuIrL] exhibited the best cytoselectivity against all the human cancer cells and was identified as the most significant cancer theranostic agent in terms of potency, selectivity, and fluorescence quantum yield. Investigation of the localization of complex [Ir2L] and [RuIrL] in the more aggressive colorectal adenocarcinoma cell HT-29 indicates that mitochondria are the key cellular target for destroying cancer cells. Mitochondrial dysfunction and G2/M phase cell cycle arrest in HT-29 cell were found to be involved in the apoptotic cell death pathway induced by the test complexes [Ir2L] and [RuIrL]. These results validate the concept that these types of complexes will be reasonably able to exert great potential for tumor diagnosis as well as therapy in the near future.
Subject(s)
Antineoplastic Agents/pharmacology , Luminescent Agents/pharmacology , Mitochondria/drug effects , Theranostic Nanomedicine , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/chemistry , Cell Cycle Checkpoints/drug effects , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Drug Screening Assays, Antitumor , HEK293 Cells , Humans , Iridium/chemistry , Iridium/pharmacology , Luminescent Agents/chemical synthesis , Luminescent Agents/chemistry , Mitochondria/metabolism , Molecular Structure , Pyridines/chemistry , Pyridines/pharmacology , Quinoxalines/chemistry , Quinoxalines/pharmacology , Ruthenium/chemistry , Ruthenium/pharmacology , Structure-Activity RelationshipABSTRACT
In this article, we report the design, synthesis, and characterization of a series of cyclometalated iridium(III) polypyridine complexes containing a perfluorobiphenyl (PFBP) moiety [Ir(N^C)2(bpy-PFBP)](PF6) (bpy-PFBP = 4-(S-(perfluoro-(1,1'-biphenyl)-4-yl)-N-mercaptoethylaminocarbonyloxymethyl)-4'-methyl-2,2'-bipyridine; HN^C = 2-phenylpyridine (Hppy) (1a), 2-(4-hydroxymethylphenyl)pyridine (Hppy-CH2OH) (2a), 2-((1,1'-biphenyl)-4-yl)pyridine (Hpppy) (3a), 2-((4'-hydroxymethyl-1,1'-biphenyl)-4-yl)pyridine (Hpppy-CH2OH) (4a), 2-phenylquinoline (Hpq) (5a), 2-(4-hydroxymethylphenyl)quinoline (Hpq-CH2OH) (6a)). Their PFBP-free counterparts [Ir(N^C)2(bpy-C4)](PF6) (bpy-C4 = 4-(N-n-butylaminocarbonyloxymethyl)-4'-methyl-2,2'-bipyridine; HN^C = Hppy (1b), Hppy-CH2OH (2b), Hpppy (3b), Hpppy-CH2OH (4b), Hpq (5b), Hpq-CH2OH (6b)) were also prepared for comparison studies. Upon irradiation, all the complexes displayed intense and long-lived greenish-yellow to orange luminescence in solutions under ambient conditions and in low-temperature alcohol glass. Reactions of the PFBP complexes with peptides containing the FCPF sequence via the π-clamp-mediated cysteine conjugation afforded luminescent peptide conjugates that exhibited rich photophysical properties. Using complex 3a as an example, we demonstrated that the conjugation of complexes to organelle-targeting peptides is an effective means to modulate their intracellular localization behavior, which was further shown to be important to their performance in photodynamic therapy. The results of this work will contribute to the development of photofunctional transition metal complexes as theranostic agents.
Subject(s)
Biphenyl Compounds/pharmacology , Coordination Complexes/pharmacology , Luminescent Agents/pharmacology , Pyridines/pharmacology , Radiation-Sensitizing Agents/pharmacology , Biphenyl Compounds/metabolism , Biphenyl Compounds/radiation effects , Cell Nucleus/metabolism , Coordination Complexes/metabolism , Coordination Complexes/radiation effects , Endoplasmic Reticulum/metabolism , HeLa Cells , Humans , Iridium/chemistry , Iridium/radiation effects , Light , Luminescent Agents/metabolism , Luminescent Agents/radiation effects , Microscopy, Confocal , Peptides/metabolism , Peptides/pharmacology , Precision Medicine , Pyridines/metabolism , Pyridines/radiation effects , Radiation-Sensitizing Agents/metabolism , Radiation-Sensitizing Agents/radiation effects , Singlet Oxygen/metabolismABSTRACT
A novel luminescent coordination polymer (CP) based on Zn(II) ions as nodes [Zn(OPY)1.5(Hbtc)]n (1), [H3btc = trimesic acid and OPY = 4,4'-(oxybis(4,1-phenylene))dipyridine] has been prepared via the solvothermal assembly of a tripodal multicarboxylic acid ligand, a bis-pyridyl ligand with V-shape containing two diverse coordination patterns as well as Zn2 + ion. The experiments of photoluminescence also reflect that the coordination polymer 1 has high sensitivity to potassium dichromate, and its quenching efficiency is Ksv of 2.12 × 104 L·mol- 1. Furthermore, its treatment activity on orthodontic root absorption was evaluated in vivo. Firstly, the CCK-8 assay was performed in this research to evaluate the biotoxicity of the synthetic compound. Next, the TNF-α and Cbfα1 released by the periodontal ligament fibroblast was determined via the ELISA test kit. In addition to this, the signaling pathway of NF-κB activation after treated with compound was measured by the RT-PCR.
Subject(s)
Coordination Complexes/pharmacology , Inflammation/drug therapy , Luminescent Agents/pharmacology , Root Resorption/drug therapy , Tooth Root/drug effects , Zinc/pharmacology , Animals , Cell Line , Coordination Complexes/chemical synthesis , Coordination Complexes/chemistry , Inflammation/metabolism , Luminescent Agents/chemical synthesis , Luminescent Agents/chemistry , Potassium Dichromate/analysis , Rats , Rats, Wistar , Root Resorption/metabolism , Tooth Root/metabolism , Zinc/chemistryABSTRACT
Chemotherapeutic agents that affect lysosomal functions represent a promising strategy for selective tumor therapy and overcoming drug resistance. In this work, two dinuclear phosphorescent rhenium(i) tricarbonyl complexes (DRe1 and DRe2) containing carboline derivatives have been synthesized, characterized and explored as potential chemotherapeutic and photodynamic therapy agents. The two dinuclear rhenium(i) complexes have good intrinsic phosphorescence properties and can label the lysosomes in cancer cells. Both dinuclear rhenium(i) complexes show potent anticancer activities toward several tested cancer cells. Moreover, they also have marked inhibitory activities against cisplatin-resistant human lung carcinoma cells (A549R), with complex DRe2 displaying 16-fold higher activity than cisplatin. Mechanism studies reveal that complex DRe2 can induce cancer cells to overproduce reactive oxygen species (ROS), including superoxide anion radicals, which leads to lysosomal membrane permeabilization (LMP) and subsequent cell apoptosis. Additionally, both DRe1 and DRe2 display significant phototoxicity under light (425 nm) irradiation in A549 cells, with phototoxicity index values of 60.8 and 41.8, respectively. Therefore, these two dinuclear organometallic rhenium(i) tricarbonyl complexes are potential anticancer agents for combined chemo-photodynamic therapy.
Subject(s)
Antineoplastic Agents/chemical synthesis , Coordination Complexes/chemical synthesis , Luminescent Agents/chemical synthesis , Photosensitizing Agents/chemical synthesis , Rhenium/chemistry , A549 Cells , Antineoplastic Agents/pharmacology , Apoptosis/drug effects , Carbolines/chemistry , Cell Membrane Permeability , Cell Proliferation/drug effects , Cisplatin/pharmacology , Coordination Complexes/pharmacology , Drug Discovery , HeLa Cells , Humans , Ligands , Luminescent Agents/pharmacology , Lysosomes/metabolism , Photochemotherapy , Photosensitizing Agents/pharmacology , Reactive Oxygen Species/metabolism , Structure-Activity RelationshipABSTRACT
A series of neutral and cationic heterotrimetallic complexes of the type fac-[Re(CO)3(bipy(CC)2-(AuL)2)X]n, where bipy(CC)2 is 4,4'-alkynyl-2,2'-bipyridine; L is either triphenylphosphine (PPh3), [1,3-bis(2,6-diisopropylphenyl)-imidazol-2-ylidene] (IPr), or tert-butyl isocyanide (CNtBu); and X is a chloride (n = 0) or acetonitrile (n = 1), were synthesized and characterized together with their Re(I) precursors, i.e., fac-[Re(CO)3(bipy(CC)2)X]n. X-ray diffraction of complexes 1, 3, and 6 corroborated the expected octahedral and linear distribution of the ligands along the Re(I) and Au(I) centers, respectively. Luminescent studies showed that all the complexes displayed a broad emission band centered between 565 and 680 nm, corresponding to a 3MLCT from the Re(I) to the diimine derivative. The presence of the gold fragment coordinated to the diimine ligand shifted in all cases the emission maxima toward higher energies. Such an emission difference could be potentially used for assessing the precise moment of interaction of the probe with the biological target if the gold fragment is implicated. Antiproliferative studies in cancer cells, A549 (lung cancer) and HeLa (cervix cancer), showed a generalized selectivity toward HeLa cells for those heterotrimetallic species incubated at longer times (72 vs 24 h). ICP-MS spectrometry revealed the greater cell internalization of cationic vs neutral species. Preliminary fluorescence microscopy experiments showed a different behavior of the complexes in HeLa and A549 cell lines. Whereas the complexes in A549 were randomly distributed in the outside of the cell, those incubated with HeLa cells were located close to the cellular membrane, suggesting some type of interaction, and possibly explaining their cellular selectivity when it comes to the antiproliferative activity displayed in the different cell lines.
Subject(s)
Antineoplastic Agents/pharmacology , Coordination Complexes/pharmacology , Luminescent Agents/pharmacology , Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/radiation effects , Cell Line, Tumor , Cell Proliferation/drug effects , Coordination Complexes/chemical synthesis , Coordination Complexes/radiation effects , Drug Screening Assays, Antitumor , Gold/chemistry , Humans , Light , Luminescent Agents/chemical synthesis , Luminescent Agents/radiation effects , Microscopy, Fluorescence , Rhenium/chemistryABSTRACT
Organic room temperature phosphorescence (RTP) materials have drawn increasing attention due to their unique features, especially the long emission lifetime for applications in biomedicine. In this review, we provide an overview of the recent developments of organic RTP materials applied in the biomedicine field. First, we introduce the basic mechanism of phosphorescence and subsequently we present various strategies of modulating the lifetime and efficiency of room temperature organic phosphorescence. Next, we summarize the progress of organic RTP materials in biological applications, including bioimaging, anti-cancer and antibacterial therapies. Finally, we provide an outlook with regard to the challenges and future perspectives in the field.
Subject(s)
Luminescent Agents/pharmacology , Luminescent Measurements/methods , Temperature , Animals , Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/therapeutic use , Antineoplastic Agents/chemistry , Antineoplastic Agents/therapeutic use , Diagnostic Imaging/methods , Humans , Luminescence , Luminescent Agents/therapeutic useABSTRACT
Near infra-red (NIR) light emitting nanomaterials had shown great promise in clinical imaging in view of negligible absorption by skin or tissue of mammalian. Thus, it demands for synthesizing stable NIR emitting nanomaterials in water environment. The present work presents synthesis of biologically acceptable luminescent near-IR emitting silver sulfide nanoparticles through an aqueous route using 2-mercaptoethanol. The prepared as-synthesized Ag2S nanoparticles exhibited bright photoluminescence with quantum yield of ca. 4%. X-ray diffraction (XRD) analysis indicated that the products were monoclinic α-Ag2S. Fourier transform infrared spectral analysis revealed that the stretching vibration at 2560â¯cm-1 responsible for S-H bond of thiol group disappeared suggesting the conjugation of 2-mercaptoethanol with Ag2S nanoparticles. In view of investigating any possible effect on genetic materials, interactions of the synthesized particles with calf thymus DNA was investigated employing Ethidium bromide (EB) as structural probe. To understand the binding mechanism, the UV-vis absorption, fluorescence and circular dichroism (CD) spectroscopic, as well as DNA melting studies measurements were carried out. The observed results confirm that nanoparticles interact with DNA through groove binding.
Subject(s)
DNA/chemistry , Luminescent Agents/pharmacology , Nanoparticles , Nucleic Acid Conformation/drug effects , Silver Compounds/pharmacology , Animals , Cattle , DNA/metabolism , Infrared Rays , Luminescent Agents/chemistry , Nanoparticles/chemistry , Nucleic Acid Denaturation/drug effects , Optical Imaging , Silver Compounds/chemistry , TemperatureABSTRACT
Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.