ABSTRACT
A unifying mechanism for abused drugs has been proposed previously from the standpoint of electron transfer. Mescaline can be accommodated within the theoretical framework based on redox cycling by the catechol metabolite with its quinone counterpart. Electron transfer may play a role in electrical effects involving the nervous system in the brain. This approach is in accord with structure activity relationships involving mescaline, abused drugs, catecholamines, and etoposide. Inefficient demethylation is in keeping with the various drug properties, such as requirement for high dosage and slow acting. There is a discussion of receptor binding, electrical effects, cell signaling and other modes of action. Mescaline is a nonselective, seretonin receptor agonist. 5-HTP receptors are involved in the stimulus properties. Research addresses the aspect of stereochemical requirements. Receptor binding may involve the proposed quinone metabolite and/or the amino sidechain via protonation. Electroencephalographic studies were performed on the effects of mescaline on men. Spikes are elicited by stimulation of a cortical area. The potentials likely originate in nonsynaptic dendritic membranes. Receptor-mediated signaling pathways were examined which affect mescaline behavior. The hallucinogen belongs to the class of 2AR agonists which regulate pathways in cortical neurons. The research identifies neural and signaling mechanisms responsible for the biological effects. Recently, another hallucinogen, psilocybin, has been included within the unifying mechanistic framework. This mushroom constituent is hydrolyzed to the phenol psilocin, also active, which is subsequently oxidized to an ET o-quinone or iminoquinone.
Subject(s)
Catechols/chemistry , Central Nervous System/drug effects , Mescaline/chemistry , Serotonin Receptor Agonists/chemistry , Catechols/metabolism , Central Nervous System/metabolism , Electron Transport , Mescaline/chemical synthesis , Mescaline/pharmacology , Oxidation-Reduction , Protein Binding , Quinones/chemistry , Quinones/metabolism , Receptors, Serotonin/chemistry , Receptors, Serotonin/metabolism , Serotonin Receptor Agonists/chemical synthesis , Serotonin Receptor Agonists/pharmacology , Signal Transduction , Structure-Activity RelationshipABSTRACT
A conformationally restricted analogue of mescaline, C-(4,5,6-trimethoxyindan-1-yl)-methanamine, was designed using a 5-HT(2A) receptor homology model. The compound possessed 3-fold higher affinity and potency than and efficacy equal to that of mescaline at the 5-HT(2A) receptor. The new analogue substituted fully for LSD in drug discrimination studies and was 5-fold more potent than mescaline. Resolution of this analogue into its enantiomers corroborated the docking experiments, showing the R-(+) isomer to have higher affinity and potency and to have efficacy similar to that of mescaline at the 5-HT(2A) receptor.
Subject(s)
Hallucinogens/chemical synthesis , Indans/chemical synthesis , Mescaline/analogs & derivatives , Mescaline/chemical synthesis , Methylamines/chemical synthesis , Receptor, Serotonin, 5-HT2A/chemistry , Serotonin 5-HT2 Receptor Agonists , Animals , Binding Sites , Cells, Cultured , Computer Simulation , Crystallography, X-Ray , Discrimination Learning/drug effects , Hallucinogens/pharmacology , Indans/pharmacology , Inositol Phosphates/biosynthesis , Lysergic Acid Diethylamide/pharmacology , Mescaline/pharmacology , Methylamines/pharmacology , Models, Molecular , Radioligand Assay , Rats , Sequence Homology, Amino Acid , Stereoisomerism , Structure-Activity RelationshipABSTRACT
A directed C-H bond activation approach to the synthesis of indans, tetralins, dihydrofurans, dihydroindoles, and other polycyclic aromatic compounds is presented. Cyclization of aromatic ketimines and aldimines containing alkenyl groups tethered at the meta position relative to the imine directing group has been achieved using (PPh3)3RhCl (Wilkinson's catalyst). The cyclization of a range of aromatic ketimines and aldimines provides bi- and tricyclic ring systems with good regioselectivity. Different ring sizes and substitution patterns can be accessed through the coupling of monosubstituted, 1,1- or 1,2-disubstituted, and trisubstituted alkenes bearing both electron-rich and electron-deficient functionality.
Subject(s)
Carbon/chemistry , Hydrogen/chemistry , Imines/chemistry , Mescaline/analogs & derivatives , Mescaline/chemical synthesisABSTRACT
[reaction: see text] A tetrahydrobis(benzofuran) mescaline analogue has been prepared in six steps and 38% overall yield from (4'-O-methyl)methyl gallate. The key step in this synthesis is a tandem cyclization reaction via directed C[bond]H activation followed by olefin insertion.
Subject(s)
Gallic Acid/analogs & derivatives , Mescaline/analogs & derivatives , Mescaline/chemical synthesis , Alkenes/chemistry , Catalysis , Cyclization , Gallic Acid/chemistry , Ruthenium/chemistryABSTRACT
Dihydrobenzofuran and tetrahydrobenzodifuran functionalities were employed as conformationally restricted bioisosteres of the aromatic methoxy groups in the prototypical hallucinogen, mescaline (1). Thus, 4-(2-aminoethyl)-6,7-dimethoxy-2,3-dihydrobenzofuran hydrochloride (8) and 1-(8-methoxy-2,3,5,6-tetrahydrobenzo[1,2-b:5,4-b']difuran-4-yl)-2- aminoethane hydrochloride (9) were prepared and evaluated along with 1 for activity in the two-lever drug discrimination (DD) paradigm in rats trained to discriminate saline from LSD tartrate (0.08 mg/kg). Also, 1, 8, and 9 were assayed for their ability to displace [3H]ketanserin from rat cortical homogenate 5-HT2A receptors and [3H]8-OH-DPAT from rat hippocampal homogenate 5-HT1A receptors. In addition, these compounds were evaluated for their ability to compete for agonist and antagonist binding to cells expressing cloned human 5-HT2A, 5-HT2B, and 5-HT2C receptors. Finally, agonist efficacy was assessed by measurement of phosphoinositide hydrolysis in NIH 3T3 cells expressing the rat 5-HT2A or 5-HT2C receptors. Although 1 fully substituted for LSD in the DD assays (ED50 = 33.5 mumol/kg), neither 8 nor 9 substituted for LSD, with just 50% of the rats administered 8 selecting the drug lever, and only 29% of the rats administered 9 selecting the drug lever. All of the test compounds had micromolar affinity for the 5-HT1A and 5-HT2A receptors in rat brain homogenate. Curiously, the rank order of affinities of the compounds at 5-HT2A sites was opposite their order of potency in the behavioral assay. An evaluation for ability to stimulate phosphoinositide turnover as a measure of functional efficacy revealed that all the compounds were of approximately equal efficacy to serotonin in 5-HT2C receptors. At 5-HT2A receptors, however, 8 and 9 were significantly less efficacious, eliciting only 61 and 45%, respectively, of the maximal response. These results are consistent with the proposed mechanism of action for phenethylamine hallucinogens, that such compounds must be full agonists at the 5-HT2A receptor subtype. In contrast to the 2,5-dimethoxy-substituted phenethylamines, where rigidification of the methoxy groups had no deleterious effect on activity, the loss of activity in the 3,4,5-trioxygenated mescaline analogues may suggest that the 3 and 5 methoxy groups must remain conformationally mobile to enable receptor activation.
Subject(s)
Benzofurans/chemical synthesis , Hallucinogens/chemical synthesis , Mescaline/analogs & derivatives , Mescaline/chemical synthesis , 3T3 Cells , Animals , Benzofurans/chemistry , Benzofurans/pharmacology , Brain/drug effects , Brain/metabolism , Hallucinogens/chemistry , Hallucinogens/pharmacology , Humans , Indicators and Reagents , Lethal Dose 50 , Lysergic Acid Diethylamide/analogs & derivatives , Lysergic Acid Diethylamide/chemical synthesis , Lysergic Acid Diethylamide/chemistry , Lysergic Acid Diethylamide/pharmacology , Mescaline/chemistry , Mescaline/pharmacology , Mice , Molecular Structure , Phosphatidylinositols/metabolism , Radioligand Assay , Rats , Receptor, Serotonin, 5-HT2A , Receptor, Serotonin, 5-HT2C , Receptors, Serotonin/drug effects , Receptors, Serotonin/physiology , Recombinant Proteins/drug effects , Recombinant Proteins/metabolism , Structure-Activity Relationship , TransfectionSubject(s)
Narcotics/chemical synthesis , Opioid-Related Disorders , Amphetamines/chemical synthesis , Cocaine/analogs & derivatives , Cocaine/chemical synthesis , Fentanyl/analogs & derivatives , Fentanyl/chemical synthesis , Humans , Meperidine/chemical synthesis , Mescaline/analogs & derivatives , Mescaline/chemical synthesis , Narcotics/adverse effectsABSTRACT
Structural juxtaposition of the 3,4,5-trimethoxyphenyl group in the same molecule with a piperazine or homopiperazine ring has been realized in a series of mescaline analogues (I-IV) as part of an investigation into the pharmacological properties of the seven-membered perhydro-1,4-diazepines (homopiperazines). The analogous six-membered piperazines were synthesized and tested as reference substances to determine whether the seven-membered ring conveyed special properties. A variety of pharmacological tests of action on the CNS showed that replacement of the amino group in mescaline by the heterocycles significantly alters the biological activity. In particular, both the piperazine and the homopiperazine derivatives displayed sedative activity to about the same extent.
Subject(s)
Central Nervous System/drug effects , Mescaline/analogs & derivatives , Animals , Chemical Phenomena , Chemistry , Hexobarbital/pharmacology , Humans , Male , Mescaline/chemical synthesis , Mescaline/pharmacology , Mice , Motor Activity/drug effects , Piperazines/chemical synthesis , Piperazines/pharmacology , Rats , Rats, Inbred Strains , Sleep/drug effects , Stereotyped Behavior/drug effects , Time FactorsABSTRACT
Two monothio analogues of mescaline and three monothio analogues of 2,3,4-trimethoxyphenethylamine (isomescaline) have been synthesized and characterized. Only the two mescaline analogues (3-and 4-thiomescaline) were found to be psychotomimetics in man, being 6 and 12 times more potent than mescaline, respectively. All five compounds can serve as substrates for bovine plasma monoamine oxidase in vitro, but no positive correlation is apparent between the extent of enzymatic degradation and human psychotomimetic potency.
Subject(s)
Hallucinogens/chemical synthesis , Mescaline/analogs & derivatives , Adult , Aged , Animals , Cattle , Chemical Phenomena , Chemistry , Humans , In Vitro Techniques , Mescaline/chemical synthesis , Mescaline/pharmacology , Middle Aged , Monoamine Oxidase InhibitorsABSTRACT
Replacement of the 4-methoxy of mescaline with higher alkyl homologues or with bromine led to increased activity at serotonin receptors in a sheep umbilical artery preparation. This activity appears correlated with lipophilicity, as measured by 1-octanol-water partition coefficients, but drops off when the 4-substituent is about five atoms in length. It is suggested that 3,4,5-trisubhe 2,4,5-substitution pattern.
Subject(s)
Mescaline/analogs & derivatives , Receptors, Serotonin/drug effects , Animals , In Vitro Techniques , Lipids , Mescaline/chemical synthesis , Mescaline/pharmacology , Sheep , Solubility , Stimulation, Chemical , Structure-Activity Relationship , Umbilical Arteries/drug effects , Umbilical Arteries/innervationABSTRACT
1-[2-(3,4,5-Trimethoxyphenyl)ethyl]-3-pyrroline, 2-(3,4,5-trimethoxybenzyl)-1,2,3,6-tetrahydropyridine, N-n-propylmescaline, N-cyclopropylmethylmescaline, and N-allylmescaline were synthesized as potential mescaline antagonists. The ability of these compounds to antagonize mescaline-induced disruption of swim behavior is also given.