ABSTRACT
No disponible
Subject(s)
Humans , Male , Aged, 80 and over , Aeromonas caviae/isolation & purification , Cholecystitis, Acute/microbiology , Venous Thrombosis/microbiology , Mesenteric Veins/microbiology , Gram-Negative Bacterial Infections/complicationsABSTRACT
Gastric mucosal inflammation causes hypergastrinemia, and gastrin receptors have been detected in several leukocyte types. We have analyzed whether gastrin affects the leukocyte-endothelial cell interactions in vivo by monitoring leukocyte rolling, adhesion, and emigration in rat mesenteric venules using intravital microscopy. Mesenteric superfusion with exogenous gastrin increased these processes in a concentration- and time-dependent manner, effects prevented by the cholecystokinin (CCK)-2 receptor antagonists (proglumide, L-365,260) but not by the CCK-1 receptor antagonist devazepide. A similar response was induced by exogenous CCK or endogenously released gastrin. CCK-2 receptors were localized in mesenteric macrophages and polymorphonuclear leukocytes. This effect of gastrin is not modulated by somatostatin and is independent of the endogenous release of histamine. To analyze whether hypergastrinemia elicited by Helicobacter pylori (HP) modulates the inflammation induced by the germ, rats were chronically administered with an extract of a CagA+/VacA+ strain of HP. This protocol increased gastrinemia and induced an inflammatory response in the rat mesentery. Blockade of CCK-2 receptors attenuated this response and induced a qualitative change in the leukocyte infiltrate suggestive of a receding inflammatory process. Our results reveal a new proinflammatory role of gastrin that seems to contribute to the maintenance of the inflammation elicited by HP components.
Subject(s)
Endothelium, Vascular/physiology , Gastrins/pharmacology , Helicobacter Infections/physiopathology , Helicobacter pylori , Inflammation/physiopathology , Leukocytes/physiology , Animals , Endothelium, Vascular/drug effects , Endothelium, Vascular/physiopathology , Leukocytes/drug effects , Male , Mesenteric Arteries/microbiology , Mesenteric Arteries/physiopathology , Mesenteric Veins/microbiology , Mesenteric Veins/physiopathology , Rats , Rats, Sprague-DawleySubject(s)
Diverticulitis, Colonic/complications , Mesenteric Veins/diagnostic imaging , Sepsis/etiology , Sigmoid Diseases/complications , Thrombophlebitis/etiology , Anti-Bacterial Agents/therapeutic use , Anticoagulants/therapeutic use , Bacteroides Infections/drug therapy , Diverticulitis, Colonic/diagnostic imaging , Diverticulitis, Colonic/drug therapy , Escherichia coli Infections/drug therapy , Heparin/therapeutic use , Humans , Male , Mesenteric Veins/microbiology , Middle Aged , Sepsis/drug therapy , Sepsis/microbiology , Sigmoid Diseases/diagnostic imaging , Sigmoid Diseases/drug therapy , Thrombophlebitis/diagnostic imaging , Thrombophlebitis/drug therapy , Thrombophlebitis/microbiology , Tomography, X-Ray Computed , Treatment OutcomeABSTRACT
A 56-year old man with non-Hodgkin's lymphoma and biliary tract endoprosthesis, developed chronic bacteremia caused by Enterococcus faecalis with high-level resistance to gentamicin and streptomycin. The sources of bacteremia were a device-associated biliary tract infection, a suppurative thrombophlebitis of the confluence of the superior mesenteric vein with the splenic vein as well as multiple liver and pancreatic abscesses. Despite antibiotic therapy and multiple drainages of abscesses, the patient died due to overwhelming infection.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Enterococcus faecalis/drug effects , Gentamicins/therapeutic use , Gram-Positive Bacterial Infections/drug therapy , Mesenteric Veins/pathology , Streptomycin/therapeutic use , Thrombosis/drug therapy , Thrombosis/microbiology , Anti-Bacterial Agents/administration & dosage , Bacteremia/complications , Bacteremia/drug therapy , Drug Resistance, Microbial , Fatal Outcome , Gentamicins/administration & dosage , Gram-Positive Bacterial Infections/microbiology , Humans , Lymphoma, Non-Hodgkin/complications , Male , Mesenteric Veins/microbiology , Microbial Sensitivity Tests , Middle Aged , Splenic Vein/microbiology , Splenic Vein/pathology , Streptomycin/administration & dosage , Suppuration/microbiology , Treatment FailureABSTRACT
A total of 42 children aged 6 months to 14 years who had the portal hypertension syndrome were studied. Blood and a lymph node were taken during a planned surgery by applying venous anastomosis. Translocation to the veins of the small and large bowels and to the mesenteric lymph nodes were recorded in 35 (83.3%), 34 (81%), and 17 (41%) patients, respectively. Translocation of aerobic bacteria (81, 78.6, and 40.5%) was more common than that of anaerobic ones (11.9, 11.9, and 4.8%) to the blood of the small and large bowels and lymph nodes, respectively, gram-negative bacteria heading the list in their frequency, Streptococci ranking next to the latter. The data on the composition of fecal microflora in the same children prior to surgery are given in the paper, which suggests that they had compensatory dysbacteriosis. A contribution of various factors (portal hypertension, operative stress, dysbacteriosis, etc.) to the development of translocation and its pathogenetic value are discussed too.
