ABSTRACT
Under the long-term effect of mineral resource exploitation, especially open-pit mining, ecosystems are severely disturbed. Constructing and optimizing urban ecological networks influenced by open-pit mines based on mine-city coordination helps integrate ecological restoration and the construction of urban ecological environments. We applied an InVEST model to Fushun City to evaluate urban ecosystem services under the influence of large open-pit mines. Twenty-one key patches important for maintaining landscape connectivity were screened as the ecological sources in the network, from which ecological resistance surfaces were constructed by combining the impacts of mines on the environment. Minimum cumulative resistance (MCR) and gravity models were then used to extract and classify ecological corridors favorable to species migration and diffusion. Fushun City had large spatial differences in ecosystem service functions, with high-value areas concentrated in the forest-rich Dongzhou District and the northern Shuncheng District. Under the influence of open-pit mining, the ecosystem service capacity of the region south of the Hunhe River was poor and lacked ecological sources. Urban ecological resistance surfaces reached a maximum in the open-pit mining area, and 210 ecological corridors were estimated using the MCR model, of which 46 were important. Only two corridors crossed the West and East open pit, forming two "ecological fracture surfaces." The Dongzhou and eastern Shuncheng districts had complex network structures and stable ecological environments. In contrast, the central and southern parts of Fushun City lacked ecological corridors owing to the influence of mining pits and gangue mountains, had simple network structures, and low connectivities with other sources. Combined with Fushun City's development plan, we propose that ecological network optimization should add new ecological source sites, reconstruct and repair ecological corridors, and upgrade ecological breakpoints. This study provides reference and basis for ecological network research in mining cities influenced by open-pit mines.
Subject(s)
Cities , Ecosystem , Mining , Mining/methods , China , Conservation of Natural Resources/methods , Models, TheoreticalABSTRACT
Underground resource exploitation has seriously damaged the surface ecological environment and underground water system. As an effective control measure, the filling mining process has greatly reduced the surface subsidence. As a branch of the filling mining process, the continuous mining and continuous backfilling (CMCB) method solves the contradiction of mining and filling in this process. The control index of filling ratio of even number lane (FRE) was presented to investigate the technical advantages of the CMCB method. The numerical analysis model was used to investigate the laws such as deformation characteristics of the surrounding rock, stress distribution, and plastic area distribution characteristics of backfill under four typical cases. As a consequence, the FRE effect law on overburden deformation and the roof control function of the backfill was disclosed, and overburden rock deformation control solutions were provided. According to the results, the overburden deformation varies dramatically when the FRE decreases, and it rises greatly when the even-numbered lane backfill (ELB) is not contacted with the roof. The contacting condition and filling condition of the odd numbered lane backfill (OLB) are connected to the distribution of stress and plastic zone. The backfill transmits the rock beam load by building a composite support system with the roof and floor rock layers, and it accomplishes the backfill's roof control function by combining the primary and secondary load-bearing and synergistic load-bearing connections between the backfills. Measures such as differential FRE, differential strength, non-uniformity of filling lane, and synergistic bearing of temporary support and backfill may help to decrease deformations and internal cracks in the surrounding rock. This measure has been successfully implemented in the field, serving as an experience for the application of the CMCB method.
Subject(s)
Environment , Groundwater , Mining , Weight-Bearing , Mining/methodsABSTRACT
Electronic scraps (e-scraps) represent an attractive raw material to mine demanded metals, as well as rare earth elements (REEs). A sequential microbial-mediated process developed in two steps was examined to recover multiple elements. First, we made use of an acidophilic bacteria consortium, mainly composed of Acidiphilium multivorum and Leptospidillum ferriphilum, isolated from acid mine drainages. The consortium was inoculated in a dissolution of e-scraps powder and cultured for 15 days. Forty-five elements were analyzed in the liquid phase over time, including silver, gold, and 15 REEs. The bioleaching efficiencies of the consortium were >99% for Cu, Co, Al, and Zn, 53% for Cd, and around 10% for Cr and Li on Day 7. The second step consisted of a microalgae-mediated uptake from e-scraps leachate. The strains used were two acidophilic extremotolerant microalgae, Euglena sp. (EugVP) and Chlamydomonas sp. (ChlSG) strains, isolated from the same extreme environment. Up to 7.3, 4.1, 1.3, and 0.7 µg by wet biomass (WB) of Zn, Al, Cu, and Mn, respectively, were uptaken by ChlSG biomass in 12 days, presenting higher efficiency than EugVP. Concerning REEs, ChlSG biouptake 14.9, 20.3, 13.7, 8.3 ng of Gd, Pr, Ce, La per WB. Meanwhile, EugVP captured 1.1, 1.5, 1.4, and 7.5, respectively. This paper shows the potential of a microbial sequential process to revalorize e-scraps and recover metals and REEs, harnessing extremotolerant microorganisms.
Subject(s)
Acidiphilium/metabolism , Bacteria/metabolism , Metals/metabolism , Mining/methods , Recycling/methods , High-Throughput Nucleotide Sequencing , Industrial Waste/analysis , Microscopy, Electron, ScanningABSTRACT
This paper proposes a cutting head optimization method based on meshing the spatial position of the picks. According to the expanded shape of the spatial mesh composed of four adjacent picks on the plane, a standard mesh shape analysis method can be established with mesh skewness, mesh symmetry, and mesh area ratio as the indicators. The traversal algorithm is used to calculate the theoretical meshing rate, pick rotation coefficient, and the variation of cutting load for the longitudinal cutting head with 2, 3, and 4 helices. The results show that the 3-helix longitudinal cutting head has better performance. By using the traversal result with maximum theoretical meshing rate as the design parameter, the longitudinal cutting head CH51 with 51 picks was designed and analyzed. The prediction model of pick consumption is established based on cutting speed, direct rock cutting volume of each pick, pick rotation coefficient, uniaxial compressive strength, and CERCHAR abrasivity index. And the rock with normal distribution characteristics of Uniaxial Compressive Strength is used for the specific energy calculating. The artificial rock wall cutting test results show that the reduction in height loss suppresses the increase in pick equivalent loss caused by the increase in mass loss, and the pick consumption in this test is only 0.037-0.054 picks/m3. In addition, the correlation between the actual pick consumption and the prediction model, and the correlation between the actual cutting specific energy and the theoretical calculation value are also analyzed. The research results show that the pick arrangement design method based on meshing pick tip spatial position can effectively reduce pick consumption and improve the rock cutting performance.
Subject(s)
Mining/methods , Algorithms , Compressive StrengthABSTRACT
In response to national policy obligations, many mining companies in Ghana have restored/reclaimed degraded mined out sites through revegetation. The area extent of such restored areas is unknown and there is also paucity of data on success of restoration, species diversity and compositional dynamics of such restored landscapes, particularly using mixed species. This study assessed stand structure, diversity and composition dynamics of sites restored with mixed species and models species abundance distribution on these sites. Three reclaimed and one control site (adjacent natural forest) were inventoried using 27, 30 x 30 m plots on the Hwini-Butre and Benso concession of the Golden Star Wassa Limited. Overall 3057 (per 24 plots) and 150 (per 3 plots) individual trees were recorded in the overstorey of the reclaimed and control sites, respectively. In all, 31 species in 13 families occurred on the reclaimed site while 61 species in 29 families occurred on the control. Species richness, abundance and diversity were significantly lower in the reclaimed sites than the control in the overstorey (p≤0.018), mid-storey (p ≤ 0.032), and understorey (p≤ 0.031). Species composition of the reclaimed and control sites were mostly dissimilar in the overstorey, midstorey, and understorey. However, the midstorey and overstorey of the reclaimed sites showed high similarity in composition (Jaccard's index = 0.817). Pioneer and shade-tolerant species were most dominant in the understorey of the control while only shade-tolerant species (mostly herbs and grasses) dominated the reclaimed sites. Species abundance distribution of both reclaimed and control sites followed the geometric series model, indicating that both sites are disturbed but at different intensities. It is concluded that reclamation with mixed species does not necessarily lead to rapid restoration of indigenous climax species on mine spoils. Nonetheless, it may lead to accomplishments of short-term goals of stabilizing and protecting landscapes while conditioning the sites for colonisation of the climax species.
Subject(s)
Forests , Soil/chemistry , Ecosystem , Environmental Monitoring/methods , Ghana , Mining/methods , Poaceae/growth & development , Trees/growth & developmentABSTRACT
The Paleogene succession of the Helmstedt Lignite Mining District in Northern Germany includes coastal peat mire records from the latest Paleocene to the middle Eocene at the southern edge of the Proto-North Sea. Therefore, it covers the different long- and short-term climate perturbations of the Paleogene greenhouse. 56 samples from three individual sections of a lower Eocene seam in the record capture the typical succession of the vegetation in a coastal wetland during a period that was not affected by climate perturbation. This allows facies-dependent vegetational changes to be distinguished from those that were climate induced. Cluster analyses and NMDS of well-preserved palynomorph assemblages reveal four successional stages in the vegetation during peat accumulation: (1) a coastal vegetation, (2) an initial mire, (3) a transitional mire, and (4) a terminal mire. Biodiversity measures show that plant diversity decreased significantly in the successive stages. The highly diverse vegetation at the coast and in the adjacent initial mire was replaced by low diversity communities adapted to wet acidic environments and nutrient deficiency. The palynomorph assemblages are dominated by elements such as Alnus (Betulaceae) or Sphagnum (Sphagnaceae). Typical tropical elements which are characteristic for the middle Eocene part of the succession are missing. This indicates that a more warm-temperate climate prevailed in northwestern Germany during the early lower Eocene.
Subject(s)
Ecosystem , Greenhouse Effect , Wetlands , Biodiversity , Climate , Fossils , Germany , Mining/methods , North Sea , Plants , SoilABSTRACT
Heavy metal sequestration from industrial wastes and agricultural soils is a long-standing challenge. This is more critical for copper since copper pollution is hazardous both for the environment and for human health. In this study, we applied an integrated approach of Darwin's theory of natural selection with bacterial genetic engineering to generate a biological system with an application for the accumulation of Cu2+ ions. A library of recombinant non-pathogenic Escherichia coli strains was engineered to express seven potential Cu2+ binding peptides encoded by a 'synthetic degenerate' DNA motif and fused to Maltose Binding Protein (MBP). Most of these peptide-MBP chimeras conferred tolerance to high concentrations of copper sulphate, and in certain cases in the order of 160-fold higher than the recognised EC50 toxic levels of copper in soils. UV-Vis spectroscopic analysis indicated a molar ratio of peptide-copper complexes, while a combination of bioinformatics-based structure modelling, Cu2+ ion docking, and MD simulations of peptide-MBP chimeras corroborated the extent of Cu2+ binding among the peptides. Further, in silico analysis predicted the peptides possessed binding affinity toward a broad range of divalent metal ions. Thus, we report on an efficient, cost-effective, and environment-friendly prototype biological system that is potentially capable of copper bioaccumulation, and which could easily be adapted for the removal of other hazardous heavy metals or the bio-mining of rare metals.
Subject(s)
Bioaccumulation/genetics , Bioengineering/methods , Copper/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Genetic Engineering/methods , Soil Pollutants/metabolism , Biodegradation, Environmental , Environmental Pollution/prevention & control , Maltose-Binding Proteins/metabolism , Mining/methods , Molecular Docking Simulation , Soil/chemistryABSTRACT
Groundwater inflow to an underground mine will seriously affect its mining plan and engineering geology safety. Groundwater models are powerful tools commonly used in the mines to develop dewatering strategies. Many mines in the Kolwezi area have been present since the 1950s, and groundwater flow patterns have been significantly influenced by mining activities. A mining plan is developed for an underground mine with overturned syncline strata in Kolwezi, Congo. Previous groundwater models using layered homogeneous media lowered model accuracies. A new three-dimensional groundwater model using FEFLOW, consisting of a combined regionally and locally geology models integrating 16 hydrogeological cross-sections and borehole logging data, are formulated to predict the underground dewatering in the study area. A 31-days pumping tests with 3 pumping wells and 28 observation wells are carried out to estimate the hydrogeological properties. The simulated water level data match the observed data rather well. Under 8 scenarios of possible well designs, the model predicts a possible dewatering capacity greater 23,900 m3/d at the initial stage of mining. The concept of the model and its application can be a reference for other mines with complex geology for mining safety in the region of interest.
Subject(s)
Environmental Monitoring/methods , Forecasting/methods , Groundwater/analysis , Computer Simulation , Democratic Republic of the Congo , Finite Element Analysis , Geology/methods , Mining/methods , Water Supply/methodsABSTRACT
Coal mining professionals in coal mining have recognized that the assessment of top coal release rate can not only improve the recovery rate of top coal, but also improve the quality of coal. But the process was often performed using a manual-based operation mode, which intensifies workload and difficulty, and is at risk of human errors. The study designs a assessment system to give the caving output ratio in top coal caving as accurately as possible based on the parameters adaptive Takagi-Sugeno (T-S) fuzzy system and the Levenberg-Marquardt (LM) algorithm. The main goal of the adaptive parameters based on LM algorithm is to construct its damping factor in the light of lowering of the objective function which is as taken as the index of termination iteration. The performance of the system is evaluated by Pearson correlation coefficient, Coefficient of Determination and relative error where the results of the Takagi-Sugeno method and the parameters adaptive Takagi-Sugeno method are compared to make the evaluation more robust and comprehensive.
Subject(s)
Coal , Expert Systems , Mining/methods , Fuzzy LogicABSTRACT
The distinctive physico-chemical features of rare earth elements (REEs) have led to an increase in demand by the global market due to their multiple uses in industrial, medical and agricultural implementations. However, the scarcity of REEs and the harsh eco-unfriendly leaching processes from primary sources beside obliviousness to their recycling from secondary sources, together with the geopolitical situation, have created the need to develop a more sustainable mining strategy. Therefore, there is a growing interest in bio-hydrometallurgy, which may contribute to the scavenging of these strategic elements from low-grade resources in an environmentally friendly and economically feasible way as with copper and gold. Several prokaryotes and eukaryotes show the ability to leach REEs, however, the success in employing these microorganisms or their products in this process relays on several biotic and abiotic factors. This review focuses on the differences made by microorganisms in REEs leaching and fundamentally explains microbes-REEs interaction.
Subject(s)
Metals, Rare Earth/isolation & purification , Mining/methods , Bacteria/growth & development , Biodegradation, Environmental , Fungi/growth & development , Recycling , Renewable EnergyABSTRACT
Acid and metalliferous drainage (AMD) remains a challenging issue for the mining sector. AMD management strategies have attempted to shift from treatment of acid leachates post-generation to more sustainable at-source prevention. Here, the efficacy of microbial-geochemical at-source control approach was investigated over a period of 84 weeks. Diverse microbial communities were stimulated using organic carbon amendment in a simulated silicate-containing sulfidic mine waste rock environment. Mineral waste in the unamended leach system generated AMD quickly and throughout the study, with known lithotrophic iron- and sulfur-oxidising microbes dominating column communities. The organic-amended mineral waste column showed suppressed metal dissolution and AMD generation. Molecular DNA-based next generation sequencing confirmed a less diverse lithotrophic community in the acid-producing control, with a more diverse microbial community under organic amendment comprising organotrophic iron/sulfur-reducers, autotrophs, hydrogenotrophs and heterotrophs. Time-series multivariate statistical analyses displayed distinct ecological patterns in microbial diversity between AMD- and non-AMD-environments. Focused ion beam-TEM micrographs and elemental mapping showed that silicate-stabilised passivation layers were successfully established across pyrite surfaces in organic-amended treatments, with these layers absent in unamended controls. Organic amendment and resulting increases in microbial abundance and diversity played an important role in sustaining these passivating layers in the long-term.
Subject(s)
Bacteria/metabolism , Industrial Waste/prevention & control , Iron/chemistry , Mining/methods , Sulfides/chemistry , Bacteria/classification , Bacteria/genetics , Bacterial Typing Techniques , Ferric Compounds/chemistry , High-Throughput Nucleotide Sequencing , Hydrogen-Ion Concentration , Oxidation-Reduction , Silicates/chemistry , Soil MicrobiologyABSTRACT
The working conditions of underground mining are complex and variable, and roof fall and rib spalling are one of the main types of accidents that can occur. Building an integrated model to evaluate the risk of roof fall and rib spalling is the foundation of mine safety. On the basis of the inherent attributes of event risk, the fuzzy evaluation set and probability of basic events are obtained by using the fuzzy fault tree analysis method based on the sample's fuzzy information. Subsequently, the likelihood of roof fall and rib spalling is determined. Consequence severity data are obtained by using the dynamic fuzzy logic method, and the consequence severity grade of roof fall and rib spalling is evaluated via the dynamic fuzzy comprehensive evaluation method. The event risk level is determined by the risk matrix method. Roof fall and rib spalling in a non-coal mine is analyzed and evaluated by using fuzzy fault tree analysis and dynamic fuzzy comprehensive evaluation. The weak links in the operation of an underground mine are identified by fuzzy fault tree analysis as "mining process, roof management, support and reinforcement." Then, the risk development trend is determined by the dynamic fuzzy comprehensive evaluation method. The risk matrix method is integrated to determine whether the risk level of the mine is "high risk, unacceptable" and expected to deteriorate in the future. The results show the validity and feasibility of the risk analysis and prediction model for roof fall and rib spalling.
Subject(s)
Fuzzy Logic , Mining , Accidents/statistics & numerical data , Mining/methods , ProbabilityABSTRACT
In Paracatu, a city in Minas Gerais State (Brazil), the gold mineral extraction produces wastes that contribute to environmental contamination by arsenic. This work describes the evaluation of arsenic concentration from soil of a gold mining area in Paracatu and the selection of arsenic resistant bacteria. In the process of culturing enrichment, 38 bacterial strains were isolated and the minimum inhibitory concentration (MIC) was determined in solid medium for each strain. Three bacterial strains named P1C1Ib, P2Ic and P2IIB were resistant to 3000â¯mgâ¯L-1 of arsenite. Analysis of 16S rDNA gene sequences revealed that these bacteria belong to Bacillus cereus and Lysinibacillus boronitolerans species. After cultivation of the strains P1C1Ib, P2Ic and P2IIIb, 69.38%-71.88% of arsenite and 82.39%-85.72% of arsenate concentrations were reduced from the culture medium, suggesting the potential application of theses strains in bioremediation processes.
Subject(s)
Arsenic/chemistry , Bioaccumulation/drug effects , Gold/chemistry , Mining/methods , Bacteria/genetics , BrazilABSTRACT
Background: Workers in the informal economy often incur exposure to well-documented occupational health hazards. Insufficient attention has been afforded to rigorously evaluating intervention programs to reduce the risks, especially in artisanal and small-scale gold mining (ASGM). Objectives: This systematic review, conducted as part of the World Health Organization's Global Plan of Action for Workers' Health, sought to assess the state of knowledge on occupational health programs and interventions for the informal artisanal and small-scale gold mining (ASGM) sector, an occupation which directly employs at least 50 million people. Methods: We used a comprehensive search strategy for four well-known databases relevant to health outcomes: PubMed, Engineering Village, OVID Medline, and Web of Science, and employed the PRISMA framework for our analysis. Findings: Ten studies met the inclusion criteria of a primary study focused on assessing the impact of interventions addressing occupational health concerns in ASGM. There were no studies evaluating or even identifying comprehensive occupational health and safety programs for this sector although target interventions addressing specific hazards exist. Major areas of intervention-education and introduction of mercury-reducing/eliminating technology were identified, and the challenges and limitations of each intervention taken into assessment. Even for these, however, there was a lack of standardization for measuring outcome or impact let alone long-term health outcomes for miners and mining communities. Conclusion: There is an urgent need for research on comprehensive occupational health programs addressing the array of hazards faced by artisanal and small-scale miners.
Subject(s)
Gold , Health Education , Informal Sector , Mining/methods , Occupational Health , Technology , Conservation of Natural Resources , Environmental Pollution/prevention & control , Extraction and Processing Industry , Humans , Mercury/adverse effects , Mercury Poisoning/prevention & control , Occupational Exposure , Water Pollution/prevention & control , World Health OrganizationABSTRACT
Safety is the fundamental guarantee for the sustainable development of mining enterprises. As the safety evaluation of mines is a complex system engineering project, consistent and inconsistent, even hesitant evaluation information may be contained simultaneously. Linguistic neutrosophic numbers (LNNs), as the extensions of linguistic terms, are effective means to entirely and qualitatively convey such evaluation information with three independent linguistic membership functions. The aim of our work is to investigate several mean operators so that the safety evaluation issues of mines are addressed under linguistic neutrosophic environment. During the safety evaluation process of mines, many influence factors should be considered, and some of them may interact with each other. To this end, the Muirhead mean (MM) operators are adopted as they are powerful tools to deal with such situation. On the other hand, to diminish the impacts of irrational data provided by evaluators, the power average (PA) operators are under consideration. Thus, with the combination of MM and PA, the power MM operators and weighted power MM operators are proposed to aggregate linguistic neutrosophic information. Meanwhile, some key points and special cases are studied. The advantages of these operators are that not only the interrelations among any number of inputs can be reflected, but also the effects of unreasonable information can be reduced. Thereafter, a new linguistic neutrosophic ranking technique based on these operators is developed to evaluate the mine safety. Moreover, in-depth discussions are made to show the robust and flexible abilities of our method. Results manifest that the proposed method is successful in dealing with mine safety evaluation issues within linguistic neutrosophic circumstances.
Subject(s)
Algorithms , Decision Making , Decision Support Techniques , Linguistics/methods , Mining/methods , Risk Assessment/methods , Fuzzy Logic , HumansABSTRACT
This study describes the preparation, characterisation and application of pelletised immobilised alginate/montmorillonite/banana peels nanocomposite (BPNC) in a fixed-bed column for continuous adsorption of rare earth elements and radioactive minerals from water. The materials was characterised by Fourier transform infrared, X-ray diffraction and scanning electron microscopy analyses. Analyses indicated that the pellets are porous and spherical in shape. FT-IR analysis showed that the functional groups responsible for the coordination of metal ions were the carboxylic (-COO-) and siloxane (Si-O-Si and Si-O-Al) groups. XRD analysis showed two additional peaks which were attributed to alginate and montmorillonite. The influence of the initial concentration, bed depth and flow rate were investigated using synthetic and real mine water in order to determine the breakthrough behaviour of both minerals. The processed bed volume, adsorbent exhaustion rate and service time, were also explored as performance indices for the adsorbent material. Furthermore, the breakthrough data were fitted to both the Thomas and Bohart-Adams models. The BPNC exhibited high affinity for U, Th, Gd and La in the real mine water sample. However, studies may still be required using waters from different environments in order to determine the robustness of BPNC.
Subject(s)
Alginates/chemical synthesis , Elements, Radioactive/isolation & purification , Metals, Rare Earth/isolation & purification , Mining , Musa/chemistry , Nanocomposites , Water Purification/methods , Adsorption , Alginates/chemistry , Alginates/pharmacokinetics , Elements, Radioactive/pharmacokinetics , Green Chemistry Technology , Humans , Materials Testing , Metals, Rare Earth/pharmacokinetics , Minerals/isolation & purification , Minerals/pharmacokinetics , Mining/methods , Nanocomposites/chemistry , Wastewater/chemistry , Water Pollutants, Chemical/isolation & purificationABSTRACT
Laboratory studies investigating subsurface microbial processes, such as metal leaching in deep ore deposits (biomining), share common and challenging obstacles, including the special environmental conditions that need to be replicated, e.g., high pressure and in some cases acidic solutions. The former requires an experimental setup suitable for pressurization up to 100 bar, while the latter demands a fluid container with high chemical resistance against corrosion and unwanted chemical reactions with the container wall. To meet these conditions for an application in the field of in situ biomining, a special flexible gold-titanium reaction cell inside a rocking high-pressure reactor was used in this study. The described system allowed simulation of in situ biomining through sulfur-driven microbial iron reduction in an anoxic, pressure-controlled, highly chemically inert experimental environment. The flexible gold-titanium reaction cell can accommodate up to 100 mL of sample solution, which can be sampled at any given time point while the system maintains the desired pressure. Experiments can be performed on timescales ranging from hours to months. Assembling the high-pressure reactor system is fairly time consuming. Nevertheless, when complex and challenging (microbiological) processes occurring in the earth's deep subsurface in chemically aggressive fluids have to be investigated in the laboratory, the advantages of this system outweigh the disadvantages. The results found that even at high pressure the microbial consortium is active, but at significantly lower metabolic rates.
Subject(s)
Gold , Microbial Consortia/physiology , Mining/methods , Pressure , Titanium , Bioreactors , Sulfur/metabolismABSTRACT
Bioflocculation is a physicochemical technique often employed to efficiently remove colloidal water pollutants. Consequently, in this study, a bioflocculant was produced, characterised and applied to remove pollutants in mine wastewater. The maximum flocculation activity of 92% was recorded at 30 °C, pH 9.0 when maltose and urea were used as energy sources and 72 h of fermentation at the inoculum size of 1% (v/v). K+ proved to be a favourable cation. The bioflocculant yield of 4 g/L was obtained. Scanning electron microscopy illustrated a hexagonal-like structure of the bioflocculant. It is composed of carbohydrates and proteins in mass proportion of 88.6 and 9.5%, respectively. The Fourier transform infrared spectrum revealed the presence of hydroxyl, amide and amino functional groups. More than 73% of the bioflocculant was obtained after exposure to 600 °C using the thermogravimetric analyser. Human embryonic kidney 293 (HEK 293) cells exhibited 95% viability after being treated with 200 µg/µL of the bioflocculant. The flocculation mechanisms were proposed to be as a result of a double layer compression by K+, chemical reactions and bridging mechanism. The removal efficiencies of 59, 72, and 75% on biological oxygen demand, chemical oxygen demand and sulphur, were obtained respectively. Thus, the bioflocculant have potential use in wastewater treatment.