ABSTRACT
Vineyard soils are frequently polluted with high concentrations of copper due application of copper sulfate in order to control fungal diseases. Bioremediation is an efficient process for the treatment of contaminated sites. Efficient copper sorption bacteria can be used for bioremoval of copper from contaminated sites. In this study, a total of 106 copper resistant bacteria were examined for resistance to copper toxicity and biosorption of copper. Eighty isolates (45 from vineyard Mollisol, 35 from Inceptisol) were obtained from EMBRAPA (Empresa Brasileira de Pesquisa Agropecuária) experimental station, Bento Gonçalves, RS, Brazil (29º09'53.92''S and 51º31'39.40''W) and 26 were obtained from copper mining waste from Caçapava do Sul, RS, Brazil (30º29'43.48''S and 53'32'37.87W). Based on resistance to copper toxicity and biosorption, 15 isolates were identified by 16S rRNA gene sequencing. Maximal copper resistance and biosorption at high copper concentration were observed with isolate N2 which removed 80 mg L-1 in 24 h. Contrarily isolate N11 (Bacillus pumilus) displayed the highest specific copper biosorption (121.82 mg/L/OD unit in 24 h). GenBank MEGABLAST analysis revealed that isolate N2 is 99 percent similar to Staphylococcus pasteuri. Results indicate that several of our isolates have potential use for bioremediation treatment of vineyards soils and mining waste contaminated with high copper concentration.
Subject(s)
Mining Acid Water/analysis , Base Sequence , Bacillus/genetics , Bacillus/isolation & purification , Copper/analysis , Copper/toxicity , Garbage , Soil Microbiology , Water Pollution , Water Purification , Biodegradation, Environmental , Methods , Soil , Methods , ToxicityABSTRACT
Las aguas ácidas de minas tienen una considerable diversidad de microorganismos eucariontes, entre ellos hongos y protistas; en particular, poco se conoce sobre la diversidad de levaduras en drenajes ácidos de minas peruanas. En el presente estudio se aisló y caracterizó la levadura 1MA9, mediante la amplificación y secuenciación del LSU D1/D2 del gen rRNA 26S. La cepa 1MA9 se identificó molecularmente como Pichia guillermondi, ésta presentó una resistencia alta a Mn2+ (>400 mM), intermedia a iones Zn2+ y Co2+ (400600 mM), pero fue sensible a iones Cu2+. Este trabajo representa el primer reporte de levaduras en ambientes acuáticos ácidos proveniente de minas peruanas.
Microbe eukaryotes like fungi and protista are common in the drainage of mines. Few information is known on microbial diversity of acid drainages of Peruvian mines. In this work we isolated and characterized the 1M9 yeast. We utilized LSU D1/D2 of the 26S rRNA gene sequence phylogenetic analyses to characterize the diversity the yeast 1MA9 isolated. The strain 1MA9 was closely related to the Pichia guilliermondii. The yeast showed high resistance to Mn2+ (>400 mM), intermediate to ions Zn2+ and Co2+ (400600 mM), but sensible to ions Cu2+. This work provides the first data on yeasts from an aquatic acid environment of Peruvian mines.
Subject(s)
Yeasts , Pichia , Mining Acid WaterABSTRACT
This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of these samples with high concentrations of metals ranged from 2.0 to 3.5. Amplified ribosomal DNA restriction analysis (ARDRA) was used to characterize the bacterial population by amplifying the 16S rRNA gene of microorganisms. A total of 39 operational taxonomic units (OTUs) were obtained from the three samples and sequenced from 384 clones. Sequence data and phylogenetic analyses showed that two dominant clones (JYC-1B, JYC-1D) in sample JYC-1 represented 69.5 percent of the total clones affiliated with Acidithiobacillus ferrooxidans (gamma-Proteobacteria), and the most dominant clones of JYC-2 and JYC-3 were affiliated with Caulobacter crescentus (alpha-Protebacteria). At the level of bacterial divisions, differences in the relative incidence of particular phylogenetic groups among the three samples and discrepancies in physicochemical characteristics suggested that the physico-chemical characteristics had an influence on phylogenetic diversity. Furthermore, the relationships between the discrepancies of physicochemical characteristics and the diversity of the bacteria communities in the three samples suggested that the biogeochemical properties, pH and concentration of soluble metal, could be key factors in controlling the structure of the bacterial population.
Subject(s)
Bacteria/classification , Mining Acid Water , Soil Drainage , China , Copper/isolation & purification , Copper/analysis , DNA, Ribosomal , Percolation , Polymerase Chain ReactionABSTRACT
Explica que cuando en los procesos tecnológicos en las explotaciones mineras no se han ejecutado técnicas preventivas es necesario poner en práctica técnicas de remediación cuya elección depende del caso a tratar ya que se deben evaluar numerosos y complejos parámetros
Subject(s)
Industrial Effluent Treatment , Mining Acid WaterABSTRACT
Explica que cuando en los procesos tecnológicos en las explotaciones mineras no se han ejecutado técnicas preventivas es necesario poner en práctica técnicas de remediación cuya elección depende del caso a tratar ya que se deben evaluar numerosos y complejos parámetros
Subject(s)
Industrial Effluent Treatment , Mining Acid WaterABSTRACT
Este trabajo está en relación a la contaminación vinculada con la acción de las aguas de drenaje ácido (AMD, acid mine drainage) provenientes de las industrias mineras que explotan carbón y/o minerales sulfatados. Describe algunos de los procesos de remediación
