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1.
Am J Physiol Heart Circ Physiol ; 314(4): H716-H723, 2018 04 01.
Article in English | MEDLINE | ID: mdl-29351468

ABSTRACT

Previous studies have indicated that central GABAergic mechanisms are involved in the heart rate (HR) responses at the onset of exercise. On the basis of previous research that showed similar increases in HR during passive and active cycling, we reasoned that the GABAergic mechanisms involved in the HR responses at the exercise onset are primarily mediated by muscle mechanoreceptor afferents. Therefore, in this study, we sought to determine whether central GABA mechanisms are involved in the muscle mechanoreflex-mediated HR responses at the onset of exercise in humans. Twenty-eight healthy subjects (14 men and 14 women) aged between 18 and 35 yr randomly performed three bouts of 5-s passive and active cycling under placebo and after oral administration of diazepam (10 mg), a benzodiazepine that produces an enhancement in GABAA activity. Beat-to-beat HR (electrocardiography) and arterial blood pressure (finger photopletysmography) were continuously measured. Electromyography of the vastus lateralis was obtained to confirm no electrical activity during passive trials. HR increased from rest under placebo and further increased after administration of diazepam in both passive (change: 12 ± 1 vs. 17 ± 1 beats/min, P < 0.01) and active (change: 14 ± 1 vs. 18 ± 1 beats/min, P < 0.01) cycling. Arterial blood pressure increased from rest similarly during all conditions ( P > 0.05). Importantly, no sex-related differences were found in any variables during experiments. These findings demonstrate, for the first time, that the GABAergic mechanisms significantly contribute to the muscle mechanoreflex-mediated HR responses at the onset of exercise in humans. NEW & NOTEWORTHY We found that passive and voluntary cycling evokes similar increases in heart rate and that these responses were enhanced after diazepam administration, a benzodiazepine that enhances GABAA activity. These findings suggest that the GABAergic system may contribute to the muscle mechanoreflex-mediated vagal withdrawal at the onset of exercise in humans.


Subject(s)
Brain/drug effects , Diazepam/administration & dosage , Exercise/physiology , GABA-A Receptor Agonists/administration & dosage , GABAergic Neurons/drug effects , Heart Rate/drug effects , Heart/innervation , Muscle Spindles/metabolism , Quadriceps Muscle/innervation , Reflex/drug effects , Adolescent , Adult , Arterial Pressure/drug effects , Bicycling , Brain/metabolism , Cross-Over Studies , Double-Blind Method , Female , GABAergic Neurons/metabolism , Humans , Male , Quadriceps Muscle/metabolism , Random Allocation , Receptors, GABA-A/drug effects , Receptors, GABA-A/metabolism , Time Factors , Young Adult , gamma-Aminobutyric Acid/metabolism
2.
Am J Physiol Heart Circ Physiol ; 305(2): H228-37, 2013 Jul 15.
Article in English | MEDLINE | ID: mdl-23709596

ABSTRACT

Myocardial stretch is an established signal that leads to hypertrophy. Myocardial stretch induces a first immediate force increase followed by a slow force response (SFR), which is a consequence of an increased Ca(2+) transient that follows the NHE1 Na(+)/H(+) exchanger activation. Carbonic anhydrase II (CAII) binds to the extreme COOH terminus of NHE1 and regulates its transport activity. We aimed to test the role of CAII bound to NHE1 in the SFR. The SFR and changes in intracellular pH (pHi) were evaluated in rat papillary muscle bathed with CO2/HCO3(-) buffer and stretched from 92% to 98% of the muscle maximal force development length for 10 min in the presence of the CA inhibitor 6-ethoxzolamide (ETZ, 100 µM). SFR control was 120 ± 3% (n = 8) of the rapid initial phase and was fully blocked by ETZ (99 ± 4%, n = 6). The SFR corresponded to a maximal increase in pHi of 0.18 ± 0.02 pH units (n = 4), and pHi changes were blocked by ETZ (0.04 ± 0.04, n = 6), as monitored by epifluorescence. NHE1/CAII physical association was examined in the SFR by coimmunoprecipitation, using muscle lysates. CAII immunoprecipitated with an anti-NHE1 antibody and the CAII immunoprecipitated protein levels increased 58 ± 9% (n = 6) upon stretch of muscles, assessed by immunoblots. The p90(RSK) kinase inhibitor SL0101-1 (10 µM) blocked the SFR of heart muscles after stretch 102 ± 2% (n = 4) and reduced the binding of CAII to NHE1, suggesting that the stretch-induced phosphorylation of NHE1 increases its binding to CAII. CAII/NHE1 interaction constitutes a component of the SFR to heart muscle stretch, which potentiates NHE1-mediated H(+) transport in the myocardium.


Subject(s)
Carbonic Anhydrase II/antagonists & inhibitors , Carbonic Anhydrase Inhibitors/pharmacology , Ethoxzolamide/pharmacology , Muscle Spindles/metabolism , Papillary Muscles/drug effects , Sodium-Hydrogen Exchangers/metabolism , Animals , Carbonic Anhydrase II/metabolism , Extracellular Signal-Regulated MAP Kinases/metabolism , Hydrogen-Ion Concentration , Immunoprecipitation , Luminescent Measurements , Male , Papillary Muscles/enzymology , Phosphorylation , Protein Binding , Protein Interaction Domains and Motifs , Protein Interaction Mapping/methods , Protein Kinase Inhibitors/pharmacology , Rats , Rats, Wistar , Ribosomal Protein S6 Kinases, 90-kDa/antagonists & inhibitors , Ribosomal Protein S6 Kinases, 90-kDa/metabolism , Sodium-Hydrogen Exchanger 1 , Time Factors
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