ABSTRACT
In contrast to abiotically formed carbonates, biogenetic carbonates have been observed to be nanocomposite, organo-mineral structures, the basic build-blocks of which are particles of quasi-uniform size (10-100 nm) organized into complex higher-order hierarchical structures, typically with highly controlled crystal-axis alignments. Some of these characteristics serve as criteria for inferring a biological origin and the state of preservation of fossil carbonate materials, and to determine whether the biomineralization process was biologically induced or controlled. Here we show that a calcium storage structure formed by the American lobster, a gastrolith initially consisting of amorphous calcium carbonate (ACC) and amorphous calcium phosphate (ACP), post-mortem can crystallize into (thus secondary) calcite with structural properties strongly influenced by the inherited organic matrix. This secondary calcite meets many structural criteria for biominerals (thus called the biomorphic calcite), but differs in trace element distributions (e.g., P and Mg). Such observations refine the capability to determine whether a fossil carbonates can be attributed to biogenic processes, with implications for the record of life on Earth and other terrestrial planets.
Subject(s)
Calcium Carbonate , Crystallization , Fossils , Calcium Carbonate/chemistry , Calcium Carbonate/metabolism , Animals , Calcium Phosphates/chemistry , Calcium Phosphates/metabolism , Nephropidae/metabolism , Nephropidae/chemistry , BiomineralizationABSTRACT
RATIONALE: Lipid correction models use elemental carbon-to-nitrogen ratios to estimate the effect of lipids on δ13 C values and provide a fast and inexpensive alternative to chemically removing lipids. However, the performance of these models varies, especially in whole-body invertebrate samples. The generation of tissue-specific lipid correction models for American lobsters, both an ecologically and an economically important species in eastern North America, will aid ecological research of this species and our understanding of the function of these models in invertebrates. METHOD: We determined the δ13 C and δ15 N values before and after lipid extraction in muscle and digestive glands of juvenile and adult lobster. We assessed the performance of four commonly used models (nonlinear, linear, natural logarithm (LN) and generalized linear model (GLM)) at estimating lipid-free δ13 C values based on the non-lipid-extracted δ13 C values and elemental C:N ratios. The accuracy of model predictions was tested using paired t-tests, and the performance of the different models was compared using the Akaike information criterion score. RESULTS: Lipid correction models accurately estimated post-lipid-extraction δ13 C values in both tissues. The nonlinear model was the least accurate for both tissues. In muscle, the three other models performed well, and in digestive glands, the LN model provided the most accurate estimates throughout the range of C:N values. In both tissues, the GLM estimates were not independent of the post-lipid-extraction δ13 C values, thus reducing their transferability to other datasets. CONCLUSIONS: Whereas previous work found that whole-body models poorly estimated the effect of lipids in invertebrates, we show that tissue-specific lipid correction models can generate accurate and precise estimates of lipid-free δ13 C values in lobster. We suggest that the tissue-specific logarithmic models presented here are the preferred models for accounting for the effect of lipid on lobster isotope ratios.
Subject(s)
Carbon Isotopes/chemistry , Lipids/chemistry , Nephropidae/chemistry , Animals , Carbon Isotopes/metabolism , Digestive System/chemistry , Digestive System/metabolism , Lipid Metabolism , Mass Spectrometry , Muscles/chemistry , Muscles/metabolism , Nephropidae/metabolism , Nitrogen Isotopes/chemistry , Nitrogen Isotopes/metabolism , Shellfish/analysisABSTRACT
The extraction of nanochitin from marine waste has attracted great industrial interest due to its unique properties, namely biodegradability, biocompatibility and as a functional reinforcing agent. Conventional acid hydrolysis isolation of nanochitin requires high temperatures and acid concentration, time and energy. Herein, for the first time, microwave irradiation method was used as an eco-friendly approach to isolate nanochitin from different sources. The isolation conditions were optimized through an experimental Box-Behnken design using surface response methodology. The data showed optimal conditions of 1 M HCl, 10.00 min and 124.75 W to obtain lobster nanocrystals; 1 M HCl, 14.34 min and 50.21 W to obtain shrimp nanocrystals; and 1 M HCl, 29.08 min and 54.08 W to obtain squid pen nanofibres, reducing time and HCl concentration. The obtained isolation yields where of 85.30, 79.92 and 80.59 % for lobster, shrimp and squid, respectively. The morphology of the nanochitins was dependent of the chitin origin, and the lengths of the nanochitins were of 314.74, 386.12 and > 900 nm for lobster, shrimp and squid pen, respectively. The thermal stability of the ensuing nanochitins was maintained after treatment. The results showed that nanochitin could be obtained by using an eco-friendly approach like microwave irradiation.
Subject(s)
Chitin/isolation & purification , Green Chemistry Technology , Microwaves , Nanofibers , Nanoparticles , Seafood , Waste Products , Animals , Carbohydrate Conformation , Chemical Fractionation , Decapodiformes/chemistry , Food Handling , Hydrochloric Acid/chemistry , Hydrogen-Ion Concentration , Hydrolysis , Nephropidae/chemistry , Penaeidae/chemistry , Structure-Activity Relationship , Time FactorsABSTRACT
Lobster species can accumulate paralytic shellfish toxins (PST) in their hepatopancreas following the consumption of toxic prey. The Southern Rock Lobster (SRL), Jasus edwardsii, industry in Tasmania, Australia, and New Zealand, collectively valued at AUD 365 M, actively manages PST risk based on toxin monitoring of lobsters in coastal waters. The SRL supply chain predominantly provides live lobsters, which includes wet holding in fishing vessels, sea-cages, or processing facilities for periods of up to several months. Survival, quality, and safety of this largely exported high-value product is a major consideration for the industry. In a controlled experiment, SRL were exposed to highly toxic cultures of Alexandrium catenella at field relevant concentrations (2 × 105 cells L-1) in an experimental aquaculture facility over a period of 21 days. While significant PST accumulation in the lobster hepatopancreas has been reported in parallel experiments feeding lobsters with toxic mussels, no PST toxin accumulated in this experiment from exposure to toxic algal cells, and no negative impact on lobster health was observed as assessed via a wide range of behavioural, immunological, and physiological measures. We conclude that there is no risk of PST accumulation, nor risk to survival or quality at the point of consumption through exposure to toxic algal cells.
Subject(s)
Aquaculture , Dinoflagellida/metabolism , Food Storage , Hepatopancreas/chemistry , Marine Toxins/analysis , Nephropidae/chemistry , Shellfish Poisoning , Shellfish/analysis , Animals , Body Burden , Dinoflagellida/growth & development , Female , Food Supply , Harmful Algal Bloom , Male , Risk Assessment , Risk FactorsABSTRACT
Commonly used to treat skin injuries in Asia, several Homalium spp. have been found to promote skin regeneration and wound healing. While ethnobotanical surveys report the use of H. bhamoense trunk bark as a wound salve, there are no studies covering bioactive properties. As impaired cutaneous healing is characterized by excessive inflammation, a series of inflammatory mediators involved in wound healing were targeted with a methanol extract obtained from H. bhamoense trunk bark. Results showed concentration-dependent inhibition of hyaluronidase and 5-lipoxygenase upon exposure to the extract, with IC50 values of 396.9 ± 25.7 and 29.0 ± 2.3 µg mL-1, respectively. H. bhamoense trunk bark extract also exerted anti-inflammatory activity by significantly suppressing the overproduction of interleukin 6 (IL-6) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages at concentrations ranging from 125 to 1000 µg mL-1, while leading to a biphasic effect on nitric oxide (NO) and tumor necrosis factor alpha (TNF-α) levels. The phenolic profile was elucidated by HPLC-DAD, being characterized by the occurrence of ellagic acid as the main constituent, in addition to a series of methylated derivatives, which might underlie the observed anti-inflammatory effects. Our findings provide in vitro data on anti-inflammatory ability of H. bhamoense trunk bark, disclosing also potential cutaneous toxicity as assessed in HaCaT keratinocytes.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Interleukin-6/adverse effects , Lipopolysaccharides/adverse effects , Macrophages/drug effects , Medicine, Traditional/methods , Nephropidae/chemistry , Plant Extracts/pharmacology , Animals , Arachidonate 5-Lipoxygenase/drug effects , Cell Survival/drug effects , Cytokines/metabolism , Herbal Medicine , Hyaluronoglucosaminidase/drug effects , Hydroxybenzoates , Inflammation Mediators/pharmacology , Inhibitory Concentration 50 , Interleukin-6/metabolism , Keratinocytes , Lipopolysaccharides/metabolism , Mice , Nitric Oxide/metabolism , RAW 264.7 Cells , Tumor Necrosis Factor-alphaABSTRACT
Ingestion of microplastics (MPs) has been documented in several marine organisms, but their occurrence in deep-sea species remains almost unknown. In this study, MPs were investigated in two economically and ecologically key crustaceans of the Mediterranean Sea, the Norwegian lobster Nephrops norvegicus and the shrimp Aristeus antennatus. Both the species were collected from 14 sites around Sardinia Island, at depths comprised between 270 and 660â¯m. A total of 89 and 63 stomachs were analysed for N. norvegicus and A. antennatus respectively, and more than 2000â¯MPs-like particles were extracted and sorted for identification and characterization by µFT-IR. In N. norvegicus, 83% of the specimens contained MPs, with an average abundance of 5.5⯱â¯0.8â¯MPs individual-1, while A. antennatus showed a lower frequency of ingestion (67%) and a lower mean number of MPs (1.66⯱â¯0.1â¯MPs individual-1). Composition and size of particles differed significantly between the two species. The non-selective feeding strategy of N. norvegicus could explain the 3-5 folds higher numbers of MPs in its stomach, which were mostly composed of films and fragments derived by polyethylene and polypropylene single-use plastic items. Contrarily, most MPs in the stomachs of A. antennatus were polyester filaments. The MPs abundance observed in N. norvegicus is among the highest detected in Mediterranean species considering both fish and invertebrates species, and provides novel insights on MPs bioavailability in deep-sea habitats. The overall results suggest that both N. norvegicus and A. antennatus, easily available in common fishery markets, could be valuable bioindicators and flagship species for plastic contamination in the deep-sea.
Subject(s)
Environmental Monitoring/methods , Microplastics/analysis , Nephropidae/chemistry , Penaeidae/chemistry , Seafood/analysis , Water Pollutants, Chemical/analysis , Animals , Ecosystem , Fisheries , Fishes , Italy , Mediterranean Sea , Norway , Polyethylene/analysis , Polypropylenes/analysis , Stomach/chemistryABSTRACT
Sous vide (SV) and high-pressure processing (HPP) are promising techniques in the development of high-quality seafood products. The objectives of this study were to evaluate the impacts of HPP on the physicochemical quality and consumer acceptance of subsequently SV-cooked lobster tails. Raw shucked lobster tails were processed at 150 or 350 MPa for 5 or 10 min. Subsequently, half were SV cooked to a core temperature of 65 °C/10 min. Texture profile analysis, shear force, color, salt soluble protein content, water-holding capacity (WHC), moisture content, and weight loss were analyzed. Pressurization at 150 MPa/10 min decreased (P < 0.05) the hardness of raw lobsters compared to non-HPP-treated controls. However, 350 MPa for 5 or 10 min increased (P < 0.05) the shear force in raw and SV-cooked samples. HPP increased (P < 0.05) the L* values but did not affect moisture content, WHC, or weight loss of raw or SV-cooked lobsters. Lobsters were subjected to consumer acceptability testing using a 9-point hedonic scale. Although panelists rated the flavor, texture, and overall liking of the 350 MPa/10 min samples higher than the control and 150 MPa/10 min samples, there were no significant differences among treatment means, indicating that physicochemical changes induced by HPP did not affect consumer acceptance. In addition, approximately 84% of panelists reported that the 350 MPa product met their expectations compared to approximately 75% for the control and 150 MPa treatments. These results suggest that HPP has the potential to be applied in combination with SV cooking to produce consumer-acceptable, value-added lobster products. PRACTICAL APPLICATION: Lobsters are an expensive menu item in restaurants. However, they are susceptible to being overcooked using conventional methods, producing a tough and rubbery texture. Sous vide cooking is reported to provide evenly cooked lobsters with a succulent and juicy texture. In this study, lobsters were sous vide cooked to reach a core temperature of 65 °C, and then maintained at that temperature for 10 min. The application of moderate processing pressures to vacuum-packaged raw lobsters prior to SV cooking altered some physicochemical attributes but has the potential to increase the availability of high-quality, minimally processed seafood with good consumer acceptability.
Subject(s)
Cooking/methods , Nephropidae/chemistry , Shellfish/analysis , Animals , Color , Consumer Behavior , Cooking/instrumentation , Humans , Nephropidae/metabolism , Taste , Temperature , VacuumABSTRACT
Contaminated sediments at a pulp mill and former chor-alkali effluent treatment facility in Nova Scotia, Canada will undergo remediation. However, baseline studies assessing contaminants in marine biota in the marine receiving environment are lacking. Historical qualitative and quantitative contaminant data in biota from Boat Harbour (a former tidal lagoon which was used to treat industrial effluent since 1967), and surrounding marine environment were reviewed to establish baseline pollution from industrial effluent and contaminated sediments. Elevated metal, dioxins and furan concentrations previously measured in marine biota needs updating to help inform pre-remediation monitoring. Selection of species, contaminants of concern and sampling locations were ad hoc and often inconsistent with environmental effects monitoring requirements under Canadian federal Pulp and Paper Effluent Regulations. These consolidated baseline data are required to determine historical impacts and to assist future monitoring during Boat Harbour sediment remediation to compare against.
Subject(s)
Aquatic Organisms/chemistry , Water Pollutants, Chemical/analysis , Animals , Biota , Bivalvia/chemistry , Dioxins/analysis , Environmental Monitoring , Estuaries , Geologic Sediments/analysis , Industry , Metals/analysis , Nephropidae/chemistry , Nova ScotiaABSTRACT
The chemical composition, structural features and bioactivities of chitosan derivatives were studied and reported here. Chitin and chitosan was extracted by chemical methods and yield was recorded 35% and 41%, moisture and ash has showed 7.4, 0.73, 1.2 and 0.79% and Ca was recorded high level (590â¯ppm). The IR spectrum has showed NH primary and secondary amines, OH group and several sugar stretching. N-acetyl glucosamine (GlcNAc), H-2 proton of glucosamine (GlcN), Anomeric protons, H-1 [GlcN (H-1D), GlcNAc (H-1A)] were noticed in 1H NMR and anomeric, methyl carbon atoms in 13C NMR. Rough surface, irregular block, crystalline with cluster and porosity structure was noticed by SEM observation. Antioxidant effect has showed good and concentration depended in four antioxidant assays. The α-amylase and ß-glucosidase enzyme inhibition effect of chitosan has showed promising and dose depend also anticoagulant potential. Chitosan could be used in pharmaceutical industry and tissue engineering.
Subject(s)
Chitosan/chemistry , Chitosan/pharmacology , Nephropidae/chemistry , Animals , Antioxidants/chemistry , Antioxidants/pharmacology , Chemical Phenomena , Chitin/chemistry , Chitosan/analogs & derivatives , Magnetic Resonance Spectroscopy , Spectroscopy, Fourier Transform Infrared , Structure-Activity RelationshipABSTRACT
Frozen storage of lobster meat (Homarus americanus) can result in undesirable quality changes that decrease consumer acceptability of these products. Current seafood industry methods use cryoprotective agents that contain phosphates including sodium tripolyphosphates (STPP). However, recent evidence suggests that cryoprotective mixtures that combine different carbohydrates and STPP can have equal or even greater cryoprotective properties compared to using STPP alone. The objective of this study was to compare the overall consumer acceptability of lobster meat stored for 6 months in different blends of these cryoprotective solutions. One hundred and seven panelists were recruited to score the acceptability of the lobster samples using nine-point hedonic scales. A check-all-that-apply (CATA) question containing 27 literature-informed, sensory descriptors was also used to identify terms frequently used to describe lobster meat. Analysis of variance analysis, indicated a significant increase for overall liking (22.1%, P < 0.0001), liking of flavor (23.6%, P < 0.0001) and texture (15.6%, P = 0.000) scores for samples stored in a novel carbohydrate blend plus sodium chloride (NaCl) and STPP compared to the water control. Subsequent penalty analysis revealed that overall liking scores were most positively associated with the attributes tender, sweet, moist and soft. Moreover, the attributes with the highest positive mean impact were more frequently used to describe lobster samples stored in solutions containing NaCl and the novel carbohydrate blend, as well as NaCl and STPP (Lobster-3 and Lobster-5 samples, respectively). PRACTICAL APPLICATION: The positive impact on the sensory quality of this novel blend of cryoprotective compounds (carbohydrates and NaCl) is proof of concept that this mixture is comparable, if not better than preservatives currently used by the seafood industry. Given the necessary regulatory approval and industry acceptance, lobster processors may consider this novel blend as a suitable alternative to freeze lobster products for up to 6 months.
Subject(s)
Cryoprotective Agents/pharmacology , Food Preservation/methods , Food Preservatives/pharmacology , Nephropidae/chemistry , Shellfish/analysis , Animals , Flavoring Agents/chemistry , Food Preservation/instrumentation , Freezing , Humans , Meat/analysis , Nephropidae/drug effects , Polyphosphates/pharmacology , Sodium Chloride/pharmacology , TasteABSTRACT
Homarus americanus, known as American lobster, is fully covered by its exoskeleton composed of rigid cuticles and soft membranes. These soft membranes are mainly located at the joints and abdomen to connect the rigid cuticles and greatly contribute to the agility of the lobster in swimming and preying. Herein, we show that the soft membrane from American lobster is a natural hydrogel (90% water) with exceptionally high toughness (up to 24.98â¯MJ/m3) and strength (up to 23.36â¯MPa), and is very insensitive to cracks. By combining experimental measurements and large-scale computational modeling, we demonstrate that the unique multilayered structure in this membrane, achieved through the ordered arrangement of chitin fibers, plays a crucial role in dissipating energy during rupture and making this membrane tough and damage tolerant. The knowledge learned from the soft membrane of natural lobsters sheds light on designing synthetic soft, yet strong and tough materials for reliable usage under extreme mechanical conditions, including a flexible armor that can provide full-body protection without sacrificing limb mobility. STATEMENT OF SIGNIFICANCE: A body armor to provide protection to people who are at risk of being hurt is only enabled by using a material that is tough and strong enough to prevent mechanical penetration. However, most modern body armors sacrifice limb protection to gain mobility, simply because none of the existing armor materials are flexible enough and they all inhibit movement of the arms and legs. Herein, we focus on the mechanics and mesoscopic structure of American lobsters' soft membrane and explore how such a natural flexible armor is designed to integrate flexibility and toughness. The knowledge learned from this study is useful to design a flexible armor for full-body protection under extreme mechanical conditions.
Subject(s)
Chitin/chemistry , Hydrogels/chemistry , Membranes, Artificial , Models, Chemical , Nephropidae/chemistry , Stress, Mechanical , AnimalsABSTRACT
Deep eutectic solvent (DES) prepared from choline chloride and four organic acid were evaluated for the extraction of chitin from lobster shell. It was found that the purity of chitins extracted with DESs was related to acid used. Purity of chitin extracted with choline chloride-malonic acid was the highest. Chitins extracted through DES treatment results in various molecular weight, which is associated with type of acid and temperature used during the treatment. For instance, chitin produced by malonic acid at 50 °C and 100 °C results in molecular weight of 312 KDa and 199 KDa respectively, whereas it extracted with malic acid at 100 °C results in 91 KDa. The physicochemical properties of chitins were characterized by FTIR, XRD, TG and SEM. Moreover, the CaCO3 was successfully converted into levulinic acid calcium salt which could be used as calcium supplement.
Subject(s)
Animal Shells/chemistry , Carboxylic Acids/chemistry , Chitin/chemistry , Choline/chemistry , Nephropidae/chemistry , Solvents/chemistry , Animals , Hot Temperature , Hydrolysis , Molecular WeightABSTRACT
We report on the characterization of the native form of an American lobster, Homarus americanus, ß-defensin-like putative antimicrobial peptide, H. americanus defensin 1 (Hoa-D1), sequenced employing top-down and bottom-up peptidomic strategies using a sensitive, chip-based nanoLC-QTOF-MS/MS instrument. The sequence of Hoa-D1 was determined by mass spectrometry; it was found to contain three disulfide bonds and an amidated C-terminus. The sequence was further validated by searching publicly-accessible H. americanus expressed sequence tag (EST) and transcriptome shotgun assembly (TSA) datasets. Hoa-D1, SYVRScSSNGGDcVYRcYGNIINGAcSGSRVccRSGGGYamide (with c representing a cysteine participating in a disulfide bond), was shown to be related to ß-defensin-like peptides previously reported from Panulirus japonicas and Panulirus argus. We found Hoa-D1 in H. americanus hemolymph, hemocytes, the supraoesophageal ganglion (brain), eyestalk ganglia, and pericardial organ extracts, as well as in the plasma of some hemolymph samples. Using discontinuous density gradient separations, we fractionatated hemocytes and localized Hoa-D1 to hemocyte sub-populations. While Hoa-D1 was detected in semigranulocytes and granulocytes using conventional proteomic strategies for analysis, the direct analysis of cell lysates exposed evidence of Hoa-D1 processing, including truncation of the C-terminal tyrosine residue, in the granulocytes, but not semigranulocytes. These measurements demonstrate the insights regarding post-translational modifications and peptide processing that can be revealed through the MS analysis of intact peptides. The identification of Hoa-D1 as a widely-distributed peptide in the lobster suggests the possibility that it may be pleiotropic, with functions in addition to its proposed role as an antimicrobial molecule in the innate immune system.
Subject(s)
Defensins/metabolism , Nephropidae/chemistry , Peptides/metabolism , Amino Acid Sequence , Animals , Computer Simulation , Defensins/chemistry , Defensins/isolation & purification , Disulfides/metabolism , Granulocytes/metabolism , Hemocytes/metabolism , Hot Temperature , Molecular Weight , Peptides/chemistry , Peptides/isolation & purification , Protein Processing, Post-Translational , Tandem Mass SpectrometryABSTRACT
Metallothioneins content was investigated in digestive gland of two wild-caught Norway lobster Nephrops norvegicus populations from the Northern Adriatic Sea, in relation to body size, season and gender. Concomitant accumulation of cadmium, mercury, arsenic, lead, chromium and manganese, reactive oxygen species concentration and energy reserves in digestive gland were also assessed. While differences between genders were not recorded, metallothioneins content seasonal trends were affected by body size. Most of parameters displayed inconsistent trends across sampling sites. Significant correlation between metallothioneins content and cadmium, arsenic and mercury concentrations was recorded only for larger lobsters. A negative correlation of reactive oxygen species concentration and metallothioneins content was observed for small, but not large lobsters. Energy reserves, in particular lipids, could considerably influence biochemical and chemical parameters variations. The present results constitute the essential baseline for future studies aimed at evaluating the N. norvegicus health in relation to metal contamination of coastal sediments.
Subject(s)
Digestive System/chemistry , Metallothionein/analysis , Metals/analysis , Nephropidae/chemistry , Nephropidae/physiology , Animals , Arsenic/analysis , Body Size , Croatia , Ecotoxicology/methods , Female , Male , Mercury/analysis , Reactive Oxygen Species/metabolism , Seasons , Shellfish/analysis , Water Pollutants, Chemical/analysisABSTRACT
This study investigated mercury contamination levels in eight commercially valuable crustacean species caught off the Central Adriatic and Tyrrhenian coasts of Italy. Total mercury levels were measured by Thermal Decomposition-Amalgamation-Atomic Absorption Spectrophotometry. Results showed a high variability among species with values ranging from 0.070 to 1.24 (mg kg-1 wet weight). The lowest mercury levels were detected in caramote prawn (Penaeus kerathurus), warty crab (Eriphia verrucosa) and European spider crab (Maja squinado), decapods living in shallow waters. Levels exceeding the limits established by the European Commission were found in species living in close contact with bottom sediments: deepwater rose shrimp (Parapenaeus longirostris), blue and red shrimp (Aristeus antennatus) and Norway lobster (Nephrops norvegicus). For shrimps, the inter-individual variability observed was mostly related to the body size, indicating the accumulation of mercury with age. An estimation of the human intake of mercury associated to the consumption of the crustaceans sampled and its comparison with the Tolerable Weekly Intake are provided.
Subject(s)
Crustacea/chemistry , Food Contamination , Mercury/analysis , Shellfish/analysis , Water Pollutants, Chemical/analysis , Animals , Brachyura/chemistry , Brachyura/growth & development , Crustacea/growth & development , Fisheries , Food Inspection , Humans , Italy , Nephropidae/chemistry , Nephropidae/growth & development , Penaeidae/chemistry , Penaeidae/growth & development , Seasons , Shellfish/standardsABSTRACT
In animals, the accepted model of carbohydrate digestion and absorption involves reduction of disaccharides into the monosaccharides glucose, fructose, and galactose followed by their individual transmembrane transport into cells. In 2011, a gene for a distinct disaccharide sucrose transporter (SCRT) was found in Drosophila melanogaster and characterized in a yeast expression system. The purpose of the present investigation was to functionally identify and characterize a putative disaccharide transporter analog in the hepatopancreas of the American lobster, Homarus americanus. Purified hepatopancreatic brush-border membrane vesicles (BBMV) were used in transport experiments using 14C-sucrose and a Millipore filter isolation technique. In the absence of sodium, an external pH of 4 significantly stimulated the uptake of 14C-sucrose compared to that occurring at pH 5, 6, or 7. At pH 7, increasing external concentrations of sodium increased 14C-sucrose uptake by BBMV in a hyperbolic fashion and this stimulation was significantly reduced when the pH was changed to 4, suggesting that both protons and sodium ions were each capable of driving the uptake of the sugar. In experiments with a variety of monosaccharides, disaccharides, and trisaccharides, used as potential inhibitors of 14C-sucrose uptake, only maltose and trehalose inhibited carrier-mediated 14C-sucrose transport. An additional experiment showed that 20 mM maltose was a competitive inhibitor of 14C-sucrose uptake. The use of a putative lobster SCRT by both maltose and trehalose is nutritionally appropriate for lobsters as they commonly digest glycogen and chitin, polymers of maltose and trehalose, respectively. These findings suggest there is a brush-border proton- or sodium-dependent, hepatopancreatic carrier process, shared by sucrose, maltose, and trehalose, that may function to absorb disaccharides that are produced from digestion of naturally occurring dietary constituents.
Subject(s)
Carrier Proteins/metabolism , Disaccharides/metabolism , Hepatopancreas/metabolism , Nephropidae/metabolism , Animals , Biological Transport/drug effects , Carbon Radioisotopes/metabolism , Carrier Proteins/antagonists & inhibitors , Carrier Proteins/chemistry , Disaccharides/pharmacology , Hydrogen-Ion Concentration , Maltose/metabolism , Maltose/pharmacology , Microvilli/metabolism , Nephropidae/chemistry , Sodium/metabolism , Sucrose/metabolism , Trehalose/metabolismABSTRACT
Recent reports of the widespread occurrence of the neurotoxin ß-N-methylamino-L-alanine (BMAA) in cyanobacteria and particularly seafood have raised concerns for public health. LC-MS/MS is currently the analytical method of choice for BMAA determinations but incomplete separation of isomeric and isobaric compounds, matrix suppression and conjugated forms are plausible limitations. In this study, capillary electrophoresis (CE) coupled with MS/MS has been developed as an alternative method for the quantitative determination of free BMAA. Using a bare fused silica capillary, a phosphate buffer (250 mM, pH 3.0) and UV detection, it was possible to separate BMAA from four isomers, but the limit of detection (LOD) of 0.25 µg mL-1 proved insufficient for analysis of typical samples. Coupling the CE to a triple quadrupole MS was accomplished using a custom sheath-flow interface. The best separation was achieved with a 5 M formic acid in water/acetonitrile (9:1) background electrolyte. Strong acid hydrolysis of lyophilized samples was used to release BMAA from conjugated forms. Field-amplified stacking after injection was achieved by lowering sample ionic strength with a cation-exchange cleanup procedure. Quantitation was accomplished using isotope dilution with deuterium-labelled BMAA as internal standard. An LOD for BMAA in solution of 0.8 ng mL-1 was attained, which was equivalent to 16 ng g-1 dry mass in samples using the specified extraction procedure. This was comparable with LC-MS/MS methods. The method displayed excellent resolution of amino acid isomers and had no interference from matrix components. The presence of BMAA in cycad, mussel and lobster samples was confirmed by CE-MS/MS, but not in an in-house cyanobacterial reference material, with quantitative results agreeing with those from LC-MS/MS. Graphical Abstract CE-MS separation and detection of BMAA, its isomers and the internal standard BMAA-d3.
Subject(s)
Amino Acids, Diamino/analysis , Electrophoresis, Capillary/methods , Food Contamination/analysis , Neurotoxins/analysis , Shellfish/analysis , Tandem Mass Spectrometry/methods , Animals , Bivalvia/chemistry , Cyanobacteria/chemistry , Cyanobacteria Toxins , Electrophoresis, Capillary/instrumentation , Equipment Design , Limit of Detection , Nephropidae/chemistry , Tandem Mass Spectrometry/instrumentationABSTRACT
In the UK, the Norway lobster (Nephrops norvegicus) supports its most important shellfish fishery. Nephrops are sold either whole, or as "tails-only" for the scampi trade. In the "tailing" process, the "head" (cephalothorax) is discarded as waste. A smaller crustacean species, the Antarctic krill Euphasia superba, represents an economically valuable industry, as its extractable oil is sold as a human dietary supplement. The aim of this study was to determine the amount and composition of the oil contained in discarded Nephrops heads and to compare its composition to the oil extracted from krill. Differences due to Geographical variation and seasonal patterns in the amount and composition of lipid were also noted. Results indicated that Nephrops head waste samples collected from more southern locations in Scotland (Clyde Sea area) contained higher levels of oil when compared to samples collected from northern locations in Iceland. Moreover, seasonal differences within the Clyde Sea area in Scotland were also observed, with oil extracted from Nephrops head waste peaking at around 11.5% during the summer months when larger and more mature females were caught by trawl. At this time of the year, the valuable fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) accounted for around 23% of the total fatty acid content in oil extracted from Nephrops head waste. A seasonal effect on EPA content was found, with higher levels obtained in the summer, while no trend was found in DHA percentages. Finally, oil from Nephrops head waste contained a higher proportion of EPA and DHA than krill oil but these fatty acids were more abundantly linked to the neutral lipids rather to than polar lipids. The characterization of lipid that could be extracted from Nephrops head waste should be seen as a first step for the commercial use of a valuable resource currently wasted. This approach is extremely relevant given the current limited supply of EPA and DHA and changes in the Common Fisheries Policy.
Subject(s)
Euphausiacea/chemistry , Lipids/chemistry , Nephropidae/chemistry , Oils/chemistry , Animals , Antarctic Regions , Dietary Supplements , Docosahexaenoic Acids/chemistry , Eicosapentaenoic Acid/chemistry , Fatty Acids/chemistry , Female , Fish Oils/chemistry , Male , Norway , Scotland , ShellfishABSTRACT
Crustacean shellfish allergy ranks among the most frequent and severe food allergies for adults, demanding rugged and sensitive analytical routine methods. The objective of this study was therefore to develop a mass spectrometric approach for the detection of contamination with shrimp and lobster, two economically important types of crustaceans, in complex food matrices. Following a biomarker approach, we identified proteotypic peptides and developed a multiple reaction monitoring (MRM) method allowing for the identification and differentiation of shrimp and lobster in the food matrix at concentrations down to 0.1%. To further enhance sensitivity, we employed the MRM-cubed (MRM(3)) mode, which allowed us to detect crustaceans down to concentrations of 25 µg/g (crustacean/food, 0.0025%). We hereby present the first mass spectrometric method for the detection of shrimp and lobster in food matrices.
Subject(s)
Allergens/chemistry , Chromatography, High Pressure Liquid/methods , Decapoda/immunology , Mass Spectrometry/methods , Nephropidae/immunology , Shellfish/analysis , Allergens/immunology , Animals , Decapoda/chemistry , Nephropidae/chemistryABSTRACT
Sulfated glycosaminoglycans (SGNL) were extracted for the first time from Norway lobster (Nephrops norvegicus) shell. The monosaccharide composition analysed by GC/MS revealed the presence of galacturonic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine. The analysis of SGNL with acetate cellulose electrophoresis in Zn-acetate revealed the presence of heparan sulfate (HS) and dermatan sulfate (DS). SGNL were evaluated for their anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. After 21h incubation, HCT116 cell proliferation was inhibited (p<0.05) between 39.7 and 54.8% at 1.5-7.5mg/mL of SGNL. SGNL don't show hemolytic activity towards bovine erythrocytes and no cytotoxicity against the normal lymphocytes. The antiproliferative efficacy of these lobster glycosaminoglycans were probably related with the higher sulfate content. SGNL demonstrated promising antiproliferative and anticoagulant potential, which may be used as a novel, effective and promising antithrombotic agent.