ABSTRACT
The genus Ochrobactrum belongs to the family Brucellaceae and its members are known to be adapted to a wide range of ecological niches. Ochrobactrum anthropi ATCC 49188T and Ochrobactrum lupini LUP21T are strains isolated from human clinical and plant root nodule samples, respectively, which share high similarity for phylogenetic markers (i.e 100â% for 16S rRNA, 99.9â% for dnaK and 99.35â% for rpoB). In this work, multiple genome average nucleotide identity (ANI) approaches, digital DNA-DNA hybridization (dDDH) and phylogenetic analysis were performed in order to investigate the taxonomic relationship between O. anthropi ATCC 49188T, O. lupini LUP21T, and other five type strains from the genus Ochrobactrum. Whole-genome comparisons demonstrated that O. lupini LUP21T and the Ochrobactrum genus type species, O. anthropi ATCC 49188T, share 97.55â% of ANIb, 98.25â% of ANIm, 97.99â% of gANI, 97.94â% of OrthoANI and 83.9â% of dDDH, which exceed the species delineation thresholds. These strains are also closely related in phylogenies reconstructed from a concatenation of 1193 sequences from single-copy ortholog genes. A review of their profiles revealed that O. anthropi ATCC 49188T and O. lupini LUP21T do not present pronounced differences at phenotypic and chemotaxonomic levels. Considering phylogenetic, genomic, phenotypic and chemotaxonomic data, O. lupini should be considered a later heterotypic synonym of O. anthropi.
Subject(s)
Ochrobactrum anthropi/classification , Ochrobactrum/classification , Bacterial Typing Techniques , Base Composition , DNA, Bacterial/genetics , Humans , Nucleic Acid Hybridization , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Ochrobactrum and Brucella are genetically related genera of the family Brucellaceae, sharing 98.8% rRNA similarity. Because of their phenotypic similarity, Ochrobactrum can be miscoded as Brucella by automated identification systems. The misidentification on blood cultures (BCs) of B. suis as O. anthropi by the VITEK 2 system is herein described. A 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a Gram-negative coccobacillus identified as O. anthropi by VITEK 2. The patient's fever persisted along with positive blood cultures despite specific antimicrobial treatment. Due to this adverse outcome, the patient was interrogated again and admitted having domestic swine. Serological tests were positive for acute brucellosis. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) of BC strains identified B. suis biovar 1. Timely identification of Brucella is essential for providing proper treatment to the patient and for advising safe handling of laboratory cultures in biological safety cabinets to prevent laboratory-acquired infection. Countries where brucellosis is endemic must be aware of this possibility.
Subject(s)
Bacteremia/diagnosis , Brucella suis/classification , Brucella suis/isolation & purification , Brucellosis/diagnosis , Diagnostic Errors , Ochrobactrum anthropi/classification , Aged , Animals , Automation, Laboratory , Bacterial Typing Techniques , Foodborne Diseases/diagnosis , Genotype , Humans , Male , Phenotype , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , Serologic Tests , SwineABSTRACT
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Subject(s)
Bacteremia/diagnosis , Bacteremia/pathology , Cystic Fibrosis/complications , Ochrobactrum anthropi/isolation & purification , Adult , Anti-Bacterial Agents/pharmacology , Bacteremia/microbiology , Female , Humans , Infant, Newborn , Infant, Premature , Microbial Sensitivity Tests , Ochrobactrum anthropi/classification , Ochrobactrum anthropi/drug effects , Ochrobactrum anthropi/geneticsABSTRACT
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.
Subject(s)
Humans , Female , Infant, Newborn , Adult , Bacteremia/diagnosis , Bacteremia/pathology , Cystic Fibrosis/complications , Ochrobactrum anthropi/isolation & purification , Anti-Bacterial Agents/pharmacology , Bacteremia/microbiology , Infant, Premature , Microbial Sensitivity Tests , Ochrobactrum anthropi/classification , Ochrobactrum anthropi/drug effects , Ochrobactrum anthropi/geneticsABSTRACT
Ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. We report the clinical and microbiological characteristics of Ochrobactrum anthropi bacteremia in a preterm patient.