ABSTRACT
Sperm-derived genetic material contributes half of the genome to the embryo, hence it's crucial to investigate which sperm parameter influences blastocyst formation in the intracytoplasmic sperm injection (ICSI) cycles with severe male infertility. The retrospective study analyzed 296 ICSI cycles with severe oligoasthenoteratozoospermia (OAT) and 99 ICSI cycles with preimplantation genetic testing for aneuploidy (PGT-A). Following the correlation analysis, data stratifications were performed in the OAT ICSI subgroup. The results showed that the matching blastocyst in the OAT ICSI cycles had inferior sperm parameters. DFI and sperm morphology had an influence on the blastocyst formation rate and the high-quality blastocysts formation rate on Day6, but no significant effect on the blastocyst development on Day 5. The high-quality blastocysts formation rate and ratio of high-quality blastocyst on Day 6 were demonstrably better in the subgroup of the teratozoospermic morphology when DFI was within the normal range. In the case of the normal sperm morphology, no statistically significant difference was found in blastocyst development, although there were numerical differences within different DFI subgroups. It was concluded that the blastocyst quality and development declined with the decreased sperm qualities.
Subject(s)
Blastocyst , Sperm Injections, Intracytoplasmic , Spermatozoa , Humans , Male , Retrospective Studies , Female , Adult , Infertility, Male/therapy , Infertility, Male/physiopathology , Pregnancy , Embryonic Development , Oligospermia/therapy , Oligospermia/physiopathologyABSTRACT
RESEARCH QUESTION: What are current practices of post-treatment fertility preservation in male childhood cancer survivors (CCS) who have not benefitted from pre-therapeutic fertility preservation in France and other European countries? DESIGN: A survey was conducted of all fertility preservation centres in France (nâ¯=â¯30) and European fertility specialists (nâ¯=â¯9) in five European countries. Eight clinical cases and 40 questions were included to assess the effect of age at diagnosis, type of treatment (alkylating-agents, orchidectomy, testicular radiotherapy) and sperm parameters on the probability of a post-treatment fertility preservation proposal. Demographic characteristics of the responding practitioner were also collected. RESULTS: Post-treatment sperm cryopreservation was proposed by 100% of fertility specialists in cases of severe oligoasthenoteratozoospermia, 77-88% in cases of moderate oligoasthenoteratozoospermia and in 65-77% in cases of sperm motility and vitality impairment. In cases of normal sperm parameters, 27-54% of fertility specialists would propose post-treatment sperm cryopreservation. These results did not differ significantly according to the type of treatment received or to responder-related factors. Practices of European specialists were also guided by sperm parameter results; 44-67% of specialists responding that they would propose sperm cryopreservation in cases of moderate to severe sperm parameter alterations. CONCLUSION: Post-treatment semen analysis could be widely proposed to CCS who have not benefitted from pre-therapeutic fertility preservation. Post-treatment fertility preservation could be proposed in cases of persistent moderate to severe sperm parameter alterations. Guidelines would be important to homogenize practices and to encourage oncologists to refer CCS for fertility assessments.
Subject(s)
Cancer Survivors , Fertility Preservation , Neoplasms , Oligospermia , Semen Preservation , Male , Humans , Young Adult , Oligospermia/therapy , Sperm Motility , Semen , Cryopreservation/methods , Spermatozoa , Fertility Preservation/methods , Semen Preservation/methods , Neoplasms/radiotherapy , Neoplasms/drug therapyABSTRACT
The extent of spermatogenic impairment on intracytoplasmic sperm injection (ICSI) outcomes and the risk of major birth defects have been little assessed. In this study, we evaluated the relationship between various spermatogenic conditions, sperm origin on ICSI outcomes, and major birth defects. A total of 934 infertile men attending the Center for Reproductive Medicine of Ren Ji Hospital (Shanghai, China) were classified into six groups: nonobstructive azoospermia (NOA; n = 84), extremely severe oligozoospermia (esOZ; n = 163), severe oligozoospermia (sOZ, n = 174), mild oligozoospermia (mOZ; n = 148), obstructive azoospermia (OAZ; n = 155), and normozoospermia (NZ; n = 210). Rates of fertilization, embryo cleavage, high-quality embryos, implantation, biochemical and clinical pregnancies, abortion, delivery, newborns, as well as major birth malformations, and other newborn outcomes were analyzed and compared among groups. The NOA group showed a statistically lower fertilization rate (68.2% vs esOZ 77.3%, sOZ 78.0%, mOZ 73.8%, OAZ 76.6%, and NZ 79.3%, all P < 0.05), but a significantly higher implantation rate (37.8%) than the groups esOZ (30.1%), sOZ (30.4%), mOZ (32.6%), and OAZ (31.0%) (all P < 0.05), which was similar to that of Group NZ (38.4%). However, there were no statistically significant differences in rates of embryo cleavage, high-quality embryos, biochemical and clinical pregnancies, abortions, deliveries, major birth malformations, and other newborn outcomes in the six groups. The results showed that NOA only negatively affects some embryological outcomes such as fertilization rate. There was no evidence of differences in other embryological and clinical outcomes with respect to sperm source or spermatogenic status. Spermatogenic failure and sperm origins do not impinge on the clinical outcomes in ICSI treatment.
Subject(s)
Female , Humans , Infant, Newborn , Male , Pregnancy , Azoospermia/therapy , China , Oligospermia/therapy , Pregnancy Rate , Retrospective Studies , Sperm Injections, Intracytoplasmic/methods , Sperm Retrieval , Spermatogenesis , SpermatozoaABSTRACT
OBJECTIVE@#To observe the clinical effect of herb-separated moxibustion on segmental conception vessel combined with low-frequency transcutaneous electrical acupoint stimulation (TEAS) for asthenospermia and oligospermia.@*METHODS@#A total of 105 patients with asthenospermia and oligospermia were randomly divided into a combination group, a TEAS group and a medication group, 35 cases in each one. In the medication group, vitamin E capsules, coenzyme Q10 capsules，@*RESULTS@#Compared before treatment, except for the sperm morphology 2 months into treatment in the medication group, the semen routine indexes 2, 3 months into treatment were improved in the 3 groups (@*CONCLUSION@#Herb-separated moxibustion on segmental conception vessel combined with low-frequency TEAS can improve semen routine, reduce sperm oxidative stress damage for patients with asthenospermia and oligospermia, and the clinical efficacy is better than the medication and TEAS.
Subject(s)
Humans , Male , Acupuncture Points , Moxibustion , Oligospermia/therapy , Sperm Motility , SpermatozoaABSTRACT
Objective: The aim of this study was to compare fertilisation, pregnancy rates and perinatal outcomes in patients undergoing intracytoplasmic sperm injection (ICSI) due to oligozoospermia. Methods: A total of 166 patients with oligozoospermia who underwent an ICSI procedure were included in the study. The subjects were divided into two groups according to the sperm retrieval technique used: group 1, ejaculated semen (n=111); group 2, surgical sperm retrieval (n=55). Results: Although the clinical pregnancy rate was lower in group 2, the difference was not statistically significant (36.4% vs. 42.3%, p=0.460). The difference between fertilisation and take-home baby rates of the groups were not significantly different, either (p=0.486, p=0.419, consecutively). Conclusion: Two different sperm retrieval techniques used for ICSI had no statistically significant difference on intracytoplasmic sperm injection outcomes in oligozoospermic patients
Objetivo: El objetivo de este estudio fue comparar la fertilización, las tasas de embarazo y los resultados perinatales en pacientes sometidos a inyección intracitoplasmática de espermatozoides (ICSI) por oligozoospermia. Métodos: Un total de 166 pacientes con oligozoospermia que se sometieron a un procedimiento ICSI se incluyeron en el estudio. Los sujetos se dividieron en dos grupos según la técnica de recuperación de espermatozoides utilizada: grupo 1, semen eyaculado (n=111); Grupo 2, recuperación quirúrgica de espermatozoides (n=55). Resultados: Aunque la tasa de embarazos clínicos fue menor en el grupo 2, la diferencia no fue estadísticamente significativa (36.4% vs. 42.3%, p=0,460). La diferencia entre las tasas de fecundación y de bebés a domicilio de los grupos tampoco fue significativamente diferente (p=0.486, p=0.419, consecutivamente). Conclusión: Dos diferentes técnicas de recuperación de espermatozoides utilizados para la ICSI no tenía ninguna diferencia estadísticamente significativa en la inyección de espermatozoides intracitoplasmáticos resultados en pacientes oligozoospermicos
Subject(s)
Humans , Male , Female , Adult , Oligospermia/therapy , Azoospermia/therapy , Sperm Injections, Intracytoplasmic/methods , Sperm Retrieval , Infertility, Male/therapy , Retrospective Studies , Spermatozoa/transplantation , Reproductive Techniques, Assisted , Ovulation Induction/methods , Treatment OutcomeABSTRACT
Oligoasthenoteratozoospermia is frequently reported in men from infertile couples. Its etiology remains, in the majority of cases, unknown with a variety of factors to contribute to its pathogenesis. The aim of this European Academy of Andrology guideline was to provide an overview of these factors and to discuss available management options.
Subject(s)
Humans , Male , Oligospermia/diagnosis , Oligospermia/therapy , Andrology/methods , Teratozoospermia/drug therapyABSTRACT
Intracytoplasmic injection with testicular spermatozoa has become a routine treatment in fertility clinics. Spermatozoa can be recovered in half of patients with nonobstructive azoospermia. The use of immature germ cells for intracytoplasmic injection has been proposed for cases in which no spermatozoa can be retrieved. However, there are low pregnancy rates following intracytoplasmic injection using round spermatids from men with no elongated spermatids or spermatozoa in their testes. The in vitro culture of immature germ cells to more mature stages has been proposed as a means to improve this poor outcome. Several years after the introduction of intracytoplasmic injection with elongating and round spermatids, uncertainty remains as to whether this approach can be considered a safe treatment option. This review outlines the clinical and scientific data regarding intracytoplasmic injection using immature germ cells and in vitro matured germ cells.
Subject(s)
Female , Humans , Male , Pregnancy , Oligospermia/therapy , Sperm Injections, Intracytoplasmic/methods , Sperm Maturation/physiology , Spermatids/physiology , Spermatids/transplantation , Spermatogenesis , Sperm Injections, IntracytoplasmicABSTRACT
A patient with a unilateral testis tumour who had been treated with radical orchiectomy was referred to our centre. He was oligospermic before orchiectomy and needed chemotherapy because of abnormal tumour markers after the aforementioned operation. He did not have any sperm suitable for freezing. Because of a lack of information about the spermatogenic abilities in the other testis, it is advisable to freeze sperm prior to orchiectomy at least in patients with semen analysis disorders (AU)
No disponible
Subject(s)
Humans , Male , Adult , Endodermal Sinus Tumor/complications , Endodermal Sinus Tumor/surgery , Freezing , Orchiectomy/rehabilitation , Semen Preservation/methods , Testicular Neoplasms/complications , Testicular Neoplasms/surgery , Oligospermia/etiology , Oligospermia/therapy , Risk AssessmentABSTRACT
Objetivo: Evaluar los resultados de ICSI realizadas con espermatozoides frescos y congelados obtenidos desde biopsias testiculares en pacientes azoospermicos desde junio 2003 a julio 2005 en el Hospital Clínico de la Universidad de Chile. Pacientes y Método: Catorce pacientes portadores de azoospermia fueron sometidos a biopsia testiculares con fines diagnósticos y terapéuticos. Las biopsias de 11 pacientes fueron realizadas el día previo a la aspiración folicular femenina y en 3 casos se utilizaron espermatozoides móviles criopreservados meses antes del procedimiento ICSI. Se cuantifico el número de pacientes en los que rescataron espermatozoides; y las tasas de fecundación y embarazo en los grupos de espermatozoides frescos y criopreservados. Resultados: En los 14 pacientes evaluados se recuperaron espermatozoides. En 3 casos se utilizaron espermatozoides congelados obtenidos de biopsias previas, y en 11 casos espermatozoides frescos para ICSI. Se obtuvieron 6 embarazos en el grupo de espermatozoides frescos y dos embarazos en el grupo de espermatozoides congelados. La tasa de fecundación en ambos grupos fue similar, 64,3 por ciento y 60,7 por ciento respectivamente. Conclusión: Nuestro trabajo demuestra que es posible obtener tasas de fecundación y embarazo semejantes con el uso de espermatozoides frescos y congelados obtenidos a partir de biopsias testiculares en pacientes azoospermicos. Recomendamos la criopreservación de espermatozoides testiculares cada vez que se realice una biopsia testicular y se encuentren espermatozoides, puesto que esto permitirá su uso posterior sin tener que someter al paciente a nuevas cirugías.
Objective: To report eight pregnancies obtained by intracitoplasmic sperm injection (ICSI) using fresh and frozen spermatozoa obtained by testicular sperm extraction (TESE) from 14 azoospermic patients. Patients and Methods: Fourteen azoospermic patients underwent TESE for ICSI. Eleven TESE were made the day previous the oocyte retrieval and three were made the previous months in association with sperm cryopreservation. We evaluated the fertilization and pregnancy rate in both groups: fresh and frozen spermatozoa. Results: Fertilization rate in both groups of patients was similar 64.3 percent and 60.7 percent respectively. Six pregnancies were obtained in patients with fresh spermatozoa and two in the frozen spermatozoa group. Conclusions: We demonstrate that is possible to obtain similar fertilization and pregnancy rates using fresh and frozen spermatozoa from TESE. To decrease the number of testicular biopsies and aborted ICSI cycles we recommend spermatozoa cryopreservation every time a diagnosis testicular biopsy is made in patients with azoospermia.
Subject(s)
Humans , Male , Female , Pregnancy , Adult , Middle Aged , Spermatozoa/physiology , Sperm Injections, Intracytoplasmic , Oligospermia/therapy , Reproductive Techniques, Assisted , Cryopreservation , Ovulation Induction , Testis/cytology , Embryo Transfer , Pregnancy RateABSTRACT
Duzentos e cinquenta e seis pacientes com azoospermiaforam estudados. Desses, 81 (36,6 por cento) eram portadores de azoospermia excretora. Dos pacientes com azoospermia excretora, 25 apresentavam agenesia bilateral do deferente, 28 obstruçäo ao nível do epidídimo e 28 azoospermia pós-vasectomia. Três pacientes com agenesia dos deferentes foram tratados através da confecçäo de espermatocele artificial. Os pacientes com obstruçäo do epidídimo foram submetidos a anastomose microcirúrgica ipidídimo-deferencial término-terminal e os pacientes vasectomizados à reconstruçäo microcirúrgica em dois planos. Nos casos de espermatocele näo houve gravidez. Nos doentes submetidos à epidídimo-vasostomia houve 60 por cento de recanalizaçäo e 30 por cento de gravidez. Nos pacientes submetidos à reversäo microcirúrgica da vasectomia houve 100 por cento de restabelecimento da permeabilidade e 78,5 por cento de gravidez