ABSTRACT
Coordinated gene expression is required for phenotypic switching between epithelial and mesenchymal phenotypes during normal development and in disease states. Trophoblast stem (TS) cells undergo epithelial-mesenchymal transition (EMT) during implantation and placentation. Mechanisms coordinating gene expression during these processes are poorly understood. We have previously demonstrated that MAP3K4-regulated chromatin modifiers CBP and HDAC6 each regulate thousands of genes during EMT in TS cells. Here we show that CBP and HDAC6 coordinate expression of only 183 genes predicted to be critical regulators of phenotypic switching. The highest-ranking co-regulated gene is the NF-κB family member Rel. Although NF-κB is primarily regulated post-transcriptionally, CBP and HDAC6 control Rel transcript levels by binding Rel regulatory regions and controlling histone acetylation. REL re-expression in mesenchymal-like TS cells induces a mesenchymal-epithelial transition. Importantly, REL forms a feedback loop, blocking HDAC6 expression and nuclear localization. Together, our work defines a developmental program coordinating phenotypic switching.
Subject(s)
Gene Expression Regulation , Histone Deacetylase 6/metabolism , MAP Kinase Kinase Kinase 4/metabolism , Oncogene Proteins v-rel/genetics , Peptide Fragments/metabolism , Phenotype , Sialoglycoproteins/metabolism , Animals , Epithelial Cells/metabolism , Epithelial-Mesenchymal Transition/genetics , Female , Humans , Male , Mice , Models, Biological , Protein Transport , Proto-Oncogene Proteins c-met/metabolism , Stem Cells/metabolism , Transcription FactorsABSTRACT
Optimal CD8 T cell immunity is orchestrated by signaling events initiated by TCR recognition of peptide Ag in concert with signals from molecules such as CD28 and 4-1BB. The molecular mechanisms underlying the temporal and spatial signaling dynamics in CD8 T cells remain incompletely understood. In this study, we show that stimulation of naive CD8 T cells with agonistic CD3 and CD28 Abs, mimicking TCR and costimulatory signals, coordinately induces 4-1BB and cRel to enable elevated cytosolic cRel:IκBα complex formation and subsequent 4-1BB-induced IκBα degradation, sustained cRel activation, heightened IL-2 production and T cell expansion. NfkbiaNES/NES CD8 T cells harboring a mutated IκBα nuclear export sequence abnormally accumulate inactive cRel:IκBα complexes in the nucleus following stimulation with agonistic anti-CD3 and anti-CD28 Abs, rendering them resistant to 4-1BB induced signaling and a disrupted chain of events necessary for efficient T cell expansion. Consequently, CD8 T cells in NfkbiaNES/NES mice poorly expand during viral infection, and this can be overcome by exogenous IL-2 administration. Consistent with cell-based data, adoptive transfer experiments demonstrated that the antiviral CD8 T cell defect in NfkbiaNES/NES mice was cell intrinsic. Thus, these results reveal that IκBα, via its unique nuclear export function, enables, rather than inhibits 4-1BB-induced cRel activation and IL-2 production to facilitate optimal CD8 T cell immunity.
Subject(s)
CD8-Positive T-Lymphocytes/immunology , Interleukin-2/metabolism , Mutation/genetics , NF-KappaB Inhibitor alpha/genetics , Oncogene Proteins v-rel/metabolism , Active Transport, Cell Nucleus , Adoptive Transfer , Animals , Antibodies, Monoclonal/metabolism , CD28 Antigens/immunology , Cells, Cultured , Lymphocyte Activation , Mice , Mice, Inbred C57BL , Mice, Transgenic , NF-KappaB Inhibitor alpha/metabolism , Oncogene Proteins v-rel/genetics , Receptors, Antigen, T-Cell/metabolism , Signal Transduction , Tumor Necrosis Factor Receptor Superfamily, Member 9/metabolismABSTRACT
Several studies have shown that microRNA-targeting is an effective strategy for the selective control of tissue-tropism and pathogenesis of both DNA and RNA viruses. However, the exploitation of microRNA-targeting for the inhibition of transformation by oncogenic viruses has not been studied. The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family of transcription factors capable of transforming primary chicken spleen and bone marrow cells. Here, by engineering the target sequence of endogenous microRNA miR-142 downstream of the v-rel gene in a Replication-Competent ALV (avian leukosis virus) long terminal repeat (LTR) with a splice acceptor (RCAS) vector and using a v-rel-induced transformation model of chicken embryonic splenocyte cultures, we show that hematopoietic-specific miR-142 can inhibit the v-rel-induced transformation, and that this inhibition effect is due to the silencing of v-rel expression. The data supports the idea that microRNA-targeting can be used to inhibit viral oncogene-induced oncogenesis.
Subject(s)
Carcinogenesis/genetics , Cell Transformation, Viral , MicroRNAs/genetics , Oncogene Proteins v-rel/genetics , Animals , Avian Leukosis Virus/genetics , Bone Marrow Cells/virology , Cells, Cultured , Chickens , Gene Silencing , NF-kappa B/metabolism , Oncogene Proteins v-rel/antagonists & inhibitors , Reticuloendotheliosis virus/genetics , Spleen/cytology , Spleen/virology , Transcription Factors/geneticsABSTRACT
Rhipicephalus microplus is an important biological vector of Anaplasma marginale, the etiological agent of bovine anaplasmosis. The knowledge of tick immune responses to control bacterial infections remains limited. In this study, we demonstrate that transcription factor Relish from the IMD signaling pathway has an important role in the control of A. marginale infection in ticks. We found that RNA-mediated silencing of Relish caused a significant increase in the number of A. marginale in the midgut and salivary glands of R. microplus. In addition, the IMD pathway regulates the expression of the gene that encodes the antimicrobial peptide (AMP) microplusin. Moreover, microplusin expression was up-regulated in the midgut (2×) and salivary glands (8×) of A. marginale infected R. microplus. Therefore, it is plausible to hypothesize that microplusin may be involved in the A. marginale control. This study provides the first evidence of IMD signaling pathway participation on the A. marginale control in R. microplus.
Subject(s)
Anaplasma marginale/immunology , Anaplasmosis/immunology , Insect Proteins/metabolism , Oncogene Proteins v-rel/metabolism , Protein-Tyrosine Kinases/metabolism , Rhipicephalus sanguineus/immunology , Salivary Glands/physiology , Agammaglobulinaemia Tyrosine Kinase , Animals , Antimicrobial Cationic Peptides/genetics , Antimicrobial Cationic Peptides/metabolism , Cattle , Immunity, Innate , Insect Proteins/genetics , Male , Oncogene Proteins v-rel/genetics , RNA, Small Interfering/genetics , Receptor Cross-Talk , Rhipicephalus sanguineus/genetics , Salivary Glands/microbiology , Signal TransductionABSTRACT
OBJECTIVES: Several rheumatoid arthritis (RA) susceptibility loci have also been found to be associated with psoriatic arthritis (PsA), demonstrating that there is a degree of genetic overlap between various autoimmune diseases. We sought to investigate whether single nucleotide polymorphisms (SNPs) mapping to previously reported RA and/or PsA susceptibility loci, including PLCL2, CCL21, REL, STAT4, CD226, PTPN22, and TYK2, are associated with risk for the two diseases in a genetically homogeneous Greek population. METHOD: This study included 392 RA patients, 126 PsA patients, and 521 healthy age- and sex-matched controls from Greece. Genotyping of the SNPs was performed with Taqman primer/probe sets. Bioinformatic analysis was performed using BlastP, PyMOL, and Maestro and Desmond. RESULTS: A significant association was detected between the GC genotype of rs34536443 (TYK2) in both the PsA and RA cohorts. The C allele of this SNP was associated with PsA only. Evidence for association with PsA was also found for the GG genotype and G allele of the rs10181656 SNP of STAT4. The TC genotype of the rs763361 SNP of CD226 was associated with PsA only. CONCLUSIONS: Genetic overlap between PsA and RA was detected for the rs34536443 SNP of the TYK2 gene within a Greek population. An association of STAT4 (rs10181656) with PsA was confirmed whereas CD226 (rs763361) was associated with PsA but not with RA, in contrast to previous reports. The different findings of this study compared to previous ones highlights the importance of comparative studies that include various ethnic or racial populations.
Subject(s)
Arthritis, Psoriatic/genetics , Arthritis, Rheumatoid/genetics , White People/genetics , Adult , Aged , Alleles , Antigens, Differentiation, T-Lymphocyte/genetics , Case-Control Studies , Chemokine CCL21/genetics , Cohort Studies , Female , Genetic Predisposition to Disease , Genotype , Genotyping Techniques , Greece , Humans , Intracellular Signaling Peptides and Proteins/genetics , Male , Middle Aged , Models, Molecular , Oncogene Proteins v-rel/genetics , Polymorphism, Single Nucleotide , Protein Tyrosine Phosphatase, Non-Receptor Type 22/genetics , STAT4 Transcription Factor/genetics , TYK2 Kinase/geneticsABSTRACT
miR5745p has been reported involved in the pathogenesis of numerous human malignancies such as colorectal and lung cancer. In this study, we aimed to explore the roles of REL and miR574 in the recurrence of prostate cancer (PCa) and to identify the underlying molecular mechanisms. Our literature search found that miR574 is regulated in cancer stem cells (CSCs), and next we used the microRNA (miRNA) database (www.mirdb.org) to find REL as a target of miR574. Luciferase assay was performed to verify the miRNA/target relationship. Oligo-transfection, realtime PCR and western blot analysis were used to support the conclusions. We validated REL to be the direct gene via luciferase reporter assay system, and realtime PCR and western blot analysis were also conducted to study the mRNA and protein expression level of REL between different groups (recurrence and nonrecurrence) or cells treated with scramble control, miR574 mimics, REL siRNA and miR574 inhibitors, indicating the negative regulatory relationship between miR574 and REL. We also investigated the relative viability of prostate CSCs when transfected with scramble control, miR574 mimics, REL siRNA and miR574 inhibitors to validate miR574 to be positively interfering with the viability of prostate CSCs. We then investigated the relative apoptosis of prostate CSCs when transfected with scramble control, miR574 mimics, REL siRNA and miR574 inhibitors. The results showed miR574 inhibited apoptosis. In conclusion, miR574 might be a novel prognostic and therapeutic target in the management of PCa recurrence.
Subject(s)
MicroRNAs/physiology , Neoplasm Recurrence, Local/metabolism , Neoplastic Stem Cells/metabolism , Oncogene Proteins v-rel/genetics , Prostatic Neoplasms/metabolism , 3' Untranslated Regions , Base Sequence , Binding Sites , Cell Line, Tumor , Down-Regulation , Gene Expression , Gene Expression Regulation, Neoplastic , Humans , Male , Neoplasm Recurrence, Local/genetics , Oncogene Proteins v-rel/metabolism , Prostatic Neoplasms/genetics , Prostatic Neoplasms/pathology , RNA InterferenceABSTRACT
Vibrio harveyi is a marine bacterial pathogen responsible for episodic abalone mortalities in France, Japan and Australia. In the European abalone, V. harveyi invades the circulatory system in a few hours after exposure and is lethal after 2 days of infection. In this study, we investigated the responses of European abalone immune cells over the first 24 h of infection. Results revealed an initial induction of immune gene expression including Rel/NF-kB, Mpeg and Clathrin. It is rapidly followed by a significant immuno-suppression characterized by reduced cellular hemocyte parameters, immune response gene expressions and enzymatic activities. Interestingly, Ferritin was overexpressed after 24 h of infection suggesting that abalone attempt to counter V. harveyi infection using soluble effectors. Immune function alteration was positively correlated with V. harveyi concentration. This study provides the evidence that V. harveyi has a hemolytic activity and an immuno-suppressive effect in the European abalone.
Subject(s)
Ferritins/metabolism , Gastropoda/immunology , Hemocytes/immunology , Vibrio Infections/immunology , Vibrio/immunology , Animals , Clathrin/genetics , Clathrin/metabolism , Europe , Ferritins/genetics , Gene Expression Regulation , Hemocytes/microbiology , Hemolysis , Immunity , Immunomodulation , NF-kappa B/genetics , NF-kappa B/metabolism , Oncogene Proteins v-rel/genetics , Oncogene Proteins v-rel/metabolismABSTRACT
Expression of inducible nitric oxide synthase (iNOS) protein by lipopolysaccharide (LPS) in BV2 microglia cells increased in a biphasic manner. Glucosamine (GlcN) selectively suppressed the late- but not early-stage iNOS response to LPS. Prolonged induction of iNOS expression by LPS was inhibited by cycloheximide, suggesting that de novo protein synthesis was required. Late-phase activation of nuclear factor-kappaB (NF-κB) activity required for sustained iNOS induction. Nuclear translocation and DNA binding of NF-κB, and Rel proteins expressions were inhibited by GlcN at later time points but not upon immediate early-stage activation by LPS. We show that GlcN selectively inhibits sustained iNOS induction by inhibiting Rel protein expression at both the mRNA and protein levels; such expression is required for prolonged iNOS induction by LPS. Our results provide mechanistic evidence that GlcN regulates inflammation, represented by iNOS. The implication of these results is that GlcN may be a potent transcriptional regulator of iNOS and other genes involved in the general inflammation process.
Subject(s)
Glucosamine/pharmacology , Lipopolysaccharides/antagonists & inhibitors , NF-kappa B/antagonists & inhibitors , Nitric Oxide Synthase Type II/biosynthesis , Oncogene Proteins v-rel/antagonists & inhibitors , Animals , Blotting, Western , Cell Line , Enzyme Induction/drug effects , Lipopolysaccharides/pharmacology , Mice , Mitogen-Activated Protein Kinases/antagonists & inhibitors , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/biosynthesis , NF-kappa B/genetics , Nitric Oxide Synthase Type II/metabolism , Oncogene Proteins v-rel/biosynthesis , Oncogene Proteins v-rel/genetics , RNA, Messenger/antagonists & inhibitors , RNA, Messenger/genetics , Signal Transduction/drug effectsABSTRACT
NF-κB, which comprises homo- and hetero-dimers of the five members of the Rel family, plays a crucial role in immunity to infection. The cRel and p50 subunits have been implicated in the development and function of the immune cells, but their in vivo importance remains poorly explored in sepsis. We aimed to study the impact of the combined loss of these two subunits on the innate response to infection in a cecal ligation and puncture model of sepsis. We have explored the possible defects in host defense, including pathogen clearance, bacterial phagocytosis and cytokine plasma release. We also performed gene profiling of cRel(-/-)p50(-/-) and wild-type LPS-stimulated peritoneal macrophages. Deficiency of cRel and p50 led to enhanced mortality to sepsis that was associated with defective macrophages phagocytosis, decreased bacterial clearance and moderate cytokine response. Transcription profile analysis revealed a common inflammatory response but a significant down-regulated transcription of genes encoding for pathogen recognition receptors and antimicrobial molecules, supporting the in vivo findings in mice. In conclusion, the cRel and p50 subunits of NF-κB play an important combined role in the innate response and are crucial for survival and pathogen clearance in polymicrobial sepsis.
Subject(s)
Macrophages/immunology , NF-kappa B p50 Subunit/metabolism , NF-kappa B/metabolism , Oncogene Proteins v-rel/metabolism , Sepsis/immunology , Animals , Cecum , Cells, Cultured , Female , Gene Expression Profiling , Humans , Immunity, Innate/genetics , Ligation , Lipopolysaccharides/immunology , Mice , Mice, Inbred C57BL , Mice, Knockout , Models, Animal , Mutant Proteins/genetics , NF-kappa B/genetics , NF-kappa B p50 Subunit/genetics , Oncogene Proteins v-rel/genetics , Punctures , Sepsis/geneticsABSTRACT
The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31(Mtb) as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31(Mtb) from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31(Mtb) expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.
Subject(s)
Bacterial Proteins/genetics , Mycobacterium tuberculosis/genetics , Up-Regulation , Bacterial Proteins/metabolism , Gene Expression Regulation, Bacterial , Mycobacterium tuberculosis/metabolism , Oncogene Proteins v-rel/genetics , Oncogene Proteins v-rel/metabolismABSTRACT
v-rel, encoded by the avian reticuloendotheliosis virus, is an acutely transforming member of the Rel/NF-κB family of transcription factors. Transformation by v-Rel is mediated by the aberrant expression of genes that are normally regulated by Rel/NF-κB. Here, we demonstrate activation of the TGF-ß/Smad signaling pathway in Rel transformation. RNA and protein levels of key TGF-ß and Smad family members (TGF-ß2, -ß3, TGF-ß type II receptor, and Smad3) are upregulated in v-Rel transformed cells with little to no change in c-Rel-expressing cells. Treatment of v-Rel transformed lymphoid cells with kinase inhibitors of the TGF-ß receptor dramatically reduces soft agar colony formation whereas addition of TGF-ß2 further promotes transformation. Moreover, Smad3 but not Smad2, is selectively activated as the downstream mediator of TGF-ß signaling. Blocking Smad3 expression or activity inhibits the oncogenic potential of v-Rel. Overall, TGF-ß/Smad signaling is activated at multiple levels and is required for the transforming ability of v-Rel.
Subject(s)
Cell Transformation, Neoplastic/metabolism , Oncogene Proteins v-rel/metabolism , Signal Transduction , Smad Proteins/metabolism , Transforming Growth Factor beta/metabolism , Animals , Cell Line , Cell Transformation, Neoplastic/genetics , Chickens , Gene Expression Regulation , Oncogene Proteins v-rel/genetics , Receptors, Transforming Growth Factor beta/genetics , Receptors, Transforming Growth Factor beta/metabolism , Reticuloendotheliosis Viruses, Avian , Smad Proteins/genetics , Transforming Growth Factor beta/geneticsABSTRACT
Cell-surface protein CD10 is a prognostic marker for diffuse large B-cell lymphoma (DLBCL), where high expression of CD10 is found in the germinal center B-cell (GCB) subtype and CD10 expression is low or absent in the activated B-cell (ABC) subtype. As compared with the GCB subtype, patients with ABC DLBCL have a poorer prognosis after standard treatment, and ABC tumor cells have higher NF-κB activity. Herein, we show that increased expression of the NF-κB target micro-RNA miR-155 is correlated with reduced expression of transcription factor PU.1 and CD10 in several B-lymphoma cell lines. Moreover, electromobility shift assays and luciferase reporter assays indicate that PU.1 can directly activate expression from the CD10 promoter. Expression of a DLBCL-derived mutant of the adaptor CARD11 (a constitutive activator of NF-κB) in the GCB-like human BJAB cell line or v-Rel in the chicken DT40 B-lymphoma cell line causes reduced expression of PU.1. The CARD11 mutant also causes a decrease in CD10 levels in BJAB cells. Similarly, overexpression of miR-155, which is known to down-regulate PU.1, leads to reduced expression of CD10 in BJAB cells. Finally, we show that CD10 expression is reduced in BJAB cells after treatment with the NF-κB inducer lipopolysaccharide (LPS). Additionally, miR-155 is induced by LPS treatment or expression of the CARD11 mutant in BJAB cells. These results point to an NF-κB-dependent mechanism for down-regulation of CD10 in B-cell lymphoma: namely, that increased NF-κB activity leads to increased miR-155, which results in decreased PU.1, and consequently reduced CD10 mRNA and protein.
Subject(s)
Biomarkers, Tumor/biosynthesis , Gene Expression Regulation, Neoplastic , Lymphoma, Large B-Cell, Diffuse/metabolism , MicroRNAs/biosynthesis , NF-kappa B/metabolism , Neprilysin/biosynthesis , Proto-Oncogene Proteins/biosynthesis , Trans-Activators/biosynthesis , Animals , B-Lymphocytes/metabolism , Biomarkers, Tumor/genetics , CARD Signaling Adaptor Proteins , Cell Line, Transformed , Cell Line, Tumor , Chickens , Germinal Center/metabolism , Guanylate Cyclase , Humans , Lipopolysaccharides/pharmacology , Lymphocyte Activation/drug effects , Lymphocyte Activation/genetics , Lymphoma, Large B-Cell, Diffuse/diagnosis , Lymphoma, Large B-Cell, Diffuse/genetics , Lymphoma, Large B-Cell, Diffuse/therapy , MicroRNAs/genetics , Mutation , NF-kappa B/genetics , Neprilysin/genetics , Oncogene Proteins v-rel/genetics , Oncogene Proteins v-rel/metabolism , Prognosis , Promoter Regions, Genetic/genetics , Proto-Oncogene Proteins/genetics , Trans-Activators/geneticsABSTRACT
Cell transformation by the v-rel oncogene is mediated by the aberrant expression of genes that are normally tightly regulated by other Rel/NF-kappaB family members. Although a number of genes inappropriately activated or suppressed by v-Rel have been identified, their contributions to the v-Rel transformation process have been poorly characterized. Here, we examine the role of individual AP-1 proteins in v-Rel-mediated transformation. v-Rel-transformed cells exhibit elevated RNA and protein expression of c-Fos, c-Jun and ATF2 and sustained repression of Fra-2. c-Fos and c-Jun are essential in both the initiation and maintenance of v-Rel-mediated transformation, whereas Fra-2 is dispensable. By employing a c-Jun dimerization mutant, we further identified Fos/Jun heterodimers as major contributors to the v-Rel transformation process. The inability of c-Rel to induce the expression of c-Fos and c-Jun contributes to its weaker oncogenic potential relative to v-Rel. Our studies also demonstrate that v-Rel may induce AP-1 members by directly upregulating gene expression (c-fos and ATF2) and by activating pathways that stimulate AP-1 activity. Although elevated expression of ATF2 is also required for v-Rel-mediated transformation, its ectopic overexpression is inhibitory. Investigating the mode of ATF2 regulation revealed a positive feedback mechanism whereby ATF2 induces p38 MAPK phosphorylation to further induce its own activity. In addition, these studies identified Ha-Ras as an effector of v-Rel-mediated transformation and reveal a novel role for ATF2 in the inhibition of the Ras-Raf-MEK-ERK signaling pathway. Overall, these studies reveal distinct and complex roles of AP-1 proteins in Rel/NF-kappaB oncogenesis.
Subject(s)
Cell Transformation, Neoplastic , NF-kappa B/metabolism , Oncogene Proteins v-rel/metabolism , Transcription Factor AP-1/metabolism , Activating Transcription Factor 2/metabolism , Animals , Cell Line , Cells, Cultured , Chick Embryo , DNA/metabolism , Extracellular Signal-Regulated MAP Kinases/metabolism , Gene Expression Regulation , Humans , MAP Kinase Signaling System , Mitogen-Activated Protein Kinase Kinases/metabolism , Oncogene Proteins v-rel/genetics , Proto-Oncogene Proteins c-fos/metabolism , Proto-Oncogene Proteins c-jun/metabolism , Proto-Oncogene Proteins p21(ras)/metabolism , raf Kinases/metabolismABSTRACT
The peptidyl-prolyl isomerase Pin1 is frequently up-regulated in human cancers in which Rel/nuclear factor-kappaB (NF-kappaB) is constitutively activated, but its role in these cancers remains to be determined, and evidence is still lacking to show that Pin1 contributes to cell transformation by Rel/NF-kappaB. Rel/NF-kappaB transcriptional and oncogenic activities are modulated by several posttranslational modifications and coregulatory proteins, and previous studies showed that cytokine treatment induces binding of Pin1 to the RelA subunit of NF-kappaB, thereby enhancing RelA nuclear localization and stability. Here we show that Pin1 associates with the Rel subunits of NF-kappaB that are implicated in leukemia/lymphomagenesis and modulates their transcriptional and oncogenic activities. Pin1 markedly enhanced transformation of primary lymphocytes by the human c-Rel protein and also increased cell transformation by the potent viral Rel/NF-kappaB oncoprotein v-Rel, in contrast to a Pin1 mutant in the WW domain involved in interaction with NF-kappaB. Pin1 promoted nuclear accumulation of Rel proteins in the absence of activating stimuli. Importantly, inhibition of Pin1 function with the pharmacologic inhibitor juglone or with Pin1-specific shRNA led to cytoplasmic relocalization of endogenous c-Rel in human lymphoma-derived cell lines, markedly interfered with lymphoma cell proliferation, and suppressed endogenous Rel/NF-kappaB-dependent gene expression. Together, these results show that Pin1 is an important regulator of Rel/NF-kappaB transforming activity and suggest that Pin1 may be a potential therapeutic target in Rel/NF-kappaB-dependent leukemia/lymphomas.
Subject(s)
Cell Transformation, Neoplastic/genetics , Oncogene Proteins v-rel/physiology , Peptidylprolyl Isomerase/physiology , Amino Acid Sequence , Animals , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Cell Proliferation/drug effects , Cells, Cultured , Chickens , Humans , Lymphoma/pathology , Molecular Sequence Data , Multigene Family/physiology , NF-kappa B/genetics , NF-kappa B/physiology , NIMA-Interacting Peptidylprolyl Isomerase , Naphthoquinones/pharmacology , Oncogene Proteins v-rel/genetics , Oncogene Proteins v-rel/metabolism , Peptidylprolyl Isomerase/antagonists & inhibitors , Peptidylprolyl Isomerase/genetics , Peptidylprolyl Isomerase/metabolism , Protein Binding , Protein Transport/drug effects , Protein Transport/genetics , Sequence Homology, Amino Acid , Up-Regulation/geneticsABSTRACT
Misregulation of REL, a nuclear factor-kappaB family transcription factor, has been implicated in several human lymphoid malignancies. REL has a conserved N-terminal DNA-binding/dimerization domain called the Rel homology domain (RHD) and a C-terminal transactivation domain (TAD). Here, we define the sequences (amino acids (aa) 323-422) between the RHD and TAD as a REL inhibitory domain (RID) because deletion of these sequences increases both REL transactivation and DNA binding. Furthermore, we have characterized two REL mRNA splice variants that encode proteins with alterations near RID: one lacking exon 9 sequences (aa 308-330; RELDelta9) and one with an exonized Alu fragment insertion of 32 aa after aa 307 (REL+Alu). Deletion of RID or exon 9-encoded sequences increases transactivation by GAL4-REL by approximately threefold. Moreover, deletion of RID or exon 9 sequences increases transactivation by full-length REL from certain kappaB site-containing promoters and increases DNA binding by REL. Deletion of RID does not affect REL's ability to transform chicken spleen cells. Reverse transcriptase-polymerase chain reaction analysis of mRNA from both primary lymphoma samples and several transformed tissue culture cell lines indicates that the RELDelta9 splice variant is preferentially expressed in lymphoma, suggesting that the REL transcript lacking exon 9 could serve as a marker for certain types of lymphoid tumors.
Subject(s)
Alternative Splicing , Biomarkers, Tumor/biosynthesis , Cell Transformation, Neoplastic/metabolism , Exons , Gene Expression Regulation, Neoplastic , Lymphoma/metabolism , Oncogene Proteins v-rel/biosynthesis , Transcriptional Activation , Alternative Splicing/genetics , Animals , Biomarkers, Tumor/genetics , Cell Transformation, Neoplastic/genetics , Cells, Cultured , Chickens , Gene Expression Regulation, Neoplastic/genetics , Humans , Lymphoma/genetics , Oncogene Proteins v-rel/genetics , Promoter Regions, Genetic/genetics , Protein Structure, Tertiary/genetics , Spleen/metabolism , Transcriptional Activation/geneticsABSTRACT
Persistent Rel/nuclear factor-kappaB (NF-kappaB) activity is a hallmark of many human cancers, and the Rel proteins are implicated in leukemia/lymphomagenesis but the mechanism is not fully understood. Microarray analysis to identify transformation-impacting genes regulated by NF-kappaB's oncogenic v-Rel and c-Rel proteins uncovered that Rel protein expression leads to transcriptional repression of key B-cell receptor (BCR) components and signaling molecules like B-cell linker (BLNK), the B-cell adaptor for phosphoinositide 3-kinase (BCAP) and immunoglobulin lambda light chain (Ig lambda), and is accompanied by a block in BCR-mediated activation of extracellular signal-regulated kinase, Akt, and c-Jun-NH(2)-kinase in response to anti-IgM. The BLNK and BCAP proteins were also down-regulated in lymphoid cells expressing a transformation-competent chimeric RelA/v-Rel protein, suggesting a correlation with the capacity of Rel proteins to transform lymphocytes. DNA-binding studies identified functional NF-kappaB-binding sites, and chromatin immunoprecipitation (ChIP) data showed binding of Rel to the endogenous blnk and bcap promoters in vivo. Importantly, restoration of either BLNK or BCAP expression strongly inhibited transformation of primary chicken lymphocytes by the potent NF-kappaB oncoprotein v-Rel. These findings are interesting because blnk and other BCR components and signaling molecules are down-regulated in primary mediastinal large B-cell lymphomas and Hodgkin's lymphomas, which depend on c-Rel for survival, and are consistent with the tumor suppressor function of BLNK. Overall, our results indicate that down-regulation of BLNK and BCAP is an important contributing factor to the malignant transformation of lymphocytes by Rel and suggest that gene repression may be as important as transcriptional activation for Rel's transforming activity.
Subject(s)
Adaptor Proteins, Signal Transducing/immunology , Lymphocytes/immunology , Oncogene Proteins v-rel/immunology , Proto-Oncogene Proteins c-rel/immunology , Transcription Factor RelA/immunology , Adaptor Proteins, Signal Transducing/genetics , Animals , B-Lymphocytes/immunology , Chickens , Chromatin/genetics , Chromatin/metabolism , DNA/genetics , Down-Regulation , Humans , Lymphocyte Activation , Mice , NF-kappa B/genetics , Oligonucleotide Array Sequence Analysis , Oncogene Proteins v-rel/genetics , Promoter Regions, Genetic , Proto-Oncogene Proteins c-rel/genetics , Transcription Factor RelA/genetics , Transcriptional Activation , TransfectionABSTRACT
The Rel/NF-kappaB and NFAT families of transcription factors are related through an N-terminal DNA-binding domain called the Rel Homology domain (RHD). Neither the RHD nor the NF-kappaB pathway has been identified in a basal (i.e., nonbilaterian) animal phylum. Using genomic and cDNA databases, we have identified two RHD domain-containing proteins from the cnidarian Nematostella vectensis: an NF-kappaB-like protein (Nv-NF-kappaB) and an NFAT-like protein (Nv-NFAT). The gene structure and RHD predicted amino acid sequence of Nv-nfkb are similar to those of the vertebrate NF-kappaB p50/p52 proteins, whereas the sequence of Nv-NFAT allows only ambiguous assignment to the NFAT family. Nv-NF-kappaB lacks the C-terminal IkappaB-like sequences present in all other NF-kappaB proteins. There are, however, two IkappaB-like genes in Nematostella encoded by loci distinct from Nv-nfkb. The separate nfkb and ikb genes of Nematostella may reflect the ancestral metazoan condition, suggesting that a gene fusion event created the nfkb genes in Drosophila and vertebrates. Nematostella also has genes that encode upstream and downstream components of the vertebrate NF-kappaB signaling pathway. Upstream components include Toll- and tumor necrosis-like receptors and ligands, adaptor proteins (Trafs, Myd88), caspases, and a TBK-like kinase. Downstream components include the NF-kappaB coactivator protein Bcl-3 and several NF-kappaB target genes. These results demonstrate that RHD-containing transcription factors and associated pathways are evolutionarily more ancient than previously known. Moreover, they suggest models for the evolutionary diversification of the insect and vertebrate Rel/NF-kappaB/IkappaB and NFAT gene families and suggest that cnidarians possess an NF-kappaB-regulated developmental or stress response pathway.
Subject(s)
Cnidaria/genetics , Oncogene Proteins v-rel/chemistry , Transcription Factors/genetics , Amino Acid Sequence , Animals , I-kappa B Proteins/genetics , Molecular Sequence Data , NF-kappa B/genetics , NFATC Transcription Factors/genetics , Oncogene Proteins v-rel/genetics , Phylogeny , Protein Structure, Tertiary , Sequence Homology, Amino Acid , Signal Transduction , Transcription Factors/chemistryABSTRACT
c-Rel is overexpressed in several B-cell lymphomas and c-rel gene overexpression can transform primary chicken lymphoid cells and induce tumors in animals. Although c-Rel is generally a stronger transcriptional activator than its viral derivative v-Rel, its oncogenic activity is significantly weaker. Among the mutations acquired during c-Rel's evolution into v-Rel are deletion of c-Rel's transactivation domain 2 (cTAD2) and mutations in cTAD1. Given the critical role of the Rel TADs in cell transformation, we investigated how mutations in c-Rel's cTAD1 and cTAD2 contribute to its oncogenicity and that of v-Rel. Mutations in cTAD2 noticeably increased c-Rel's transforming activity by promoting its nuclear localization and gene-specific transactivation, despite an overall decrease in kappaB site-dependent transactivation potency. Conversely, substitution of vTAD by cTAD1 increased v-Rel's transactivation and transforming efficiencies, whereas its substitution by the stronger cTAD2 compromised activation of mip-1beta but not irf-4 and was detrimental to cell transformation. These results suggest that the Rel TADs differentially contribute to gene-specific activation and that an optimal range of transcription potency is necessary for efficient transformation. These findings may have important implications for understanding how Rel TAD mutations can lead to a more oncogenic phenotype.
Subject(s)
Cell Nucleus/metabolism , Cell Transformation, Neoplastic/genetics , Proto-Oncogene Proteins c-rel/genetics , Transcriptional Activation , Amino Acid Sequence , Animals , Binding Sites , Cells, Cultured , Chickens , Electrophoretic Mobility Shift Assay , Humans , Immunoprecipitation , Lymphocytes/cytology , Lymphocytes/metabolism , Molecular Sequence Data , Mutation , Oncogene Proteins v-rel/genetics , Oncogene Proteins v-rel/metabolism , Protein Binding , Proto-Oncogene Proteins c-rel/metabolism , Transcription, GeneticABSTRACT
Studies on Drosophila immunity have focused on the humoral response, whereas less is known about the Drosophila cellular immunity. Here we show that mutants that lack the Drosophila Rel/NF-kappaB proteins Dorsal and Dif have very few blood cells, are constitutively infected by opportunistic microbes, and die from infection as larvae. When the double mutants are grown in microbe-free conditions, the animals are rescued from chronic infection and many survive to adult stages. Thus, Dif and Dorsal are required for survival because they protect the animal from infection by microbes from the environment. Specific expression of Dif or dorsal in the blood cell lineage is sufficient to restore blood cell number, clear microbes, and allow survival to the adult stage. These findings demonstrate that the cellular immune response is essential for the ability of Drosophila to survive in their standard laboratory environment, and that Dif and Dorsal control crucial aspects of the cellular immune response, including blood cell survival and the ability to fight off microbial infection.
Subject(s)
DNA-Binding Proteins/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/immunology , Drosophila melanogaster/metabolism , NF-kappa B/metabolism , Nuclear Proteins/metabolism , Oncogene Proteins v-rel/metabolism , Phosphoproteins/metabolism , Transcription Factors/metabolism , Animals , Cell Proliferation , DNA-Binding Proteins/genetics , Drosophila Proteins/genetics , Drosophila melanogaster/genetics , Drosophila melanogaster/microbiology , Escherichia coli/immunology , Escherichia coli Infections/genetics , Escherichia coli Infections/immunology , Escherichia coli Infections/metabolism , Escherichia coli Infections/microbiology , Hemocytes/cytology , Hemocytes/metabolism , Immunity, Cellular , Larva/genetics , Larva/immunology , Larva/metabolism , Mutation/genetics , NF-kappa B/genetics , Nuclear Proteins/genetics , Oncogene Proteins v-rel/genetics , Opportunistic Infections/immunology , Opportunistic Infections/prevention & control , Phagocytes , Phosphoproteins/genetics , Survival Rate , Transcription Factors/geneticsABSTRACT
Telomerase is activated during the transformation of lymphoid cells and fibroblasts by v-Rel, the oncogenic member of the Rel/NF-kappaB family of transcription factors. v-Rel-transformed cell lines have longer telomeres than untransformed chicken lymphoid cells and have high levels of telomerase activity. v-Rel-mediated activation of telomerase is achieved by multiple mechanisms. The expression of the gene encoding the catalytic subunit of telomerase (TERT) was directly upregulated by v-Rel. Moreover, the expression of v-Rel altered the ratio of alternatively spliced and full-length TERT transcripts in favor of the full-length forms. The activation of telomerase by v-Rel in lymphocytes was also accompanied by inactivation of nuclear inhibitors. The inhibition of telomerase activity in v-Rel-transformed cell lines led to apoptosis within 24 h. The expression of v-Rel in a macrophage cell line resulted in elevated levels of reactive oxygen species (ROS), increased telomerase activity, and increased sensitivity to telomerase inhibitors. In contrast, the ectopic expression of TERT decreased the extent of apoptosis induced by ROS. The activation of telomerase by v-Rel may, therefore, partially protect the transformed cells from apoptosis induced by ROS.