ABSTRACT
A new globoscinic acid derivative, aspertubin A (1) along with four known compounds, were obtained from the co-culture of Aspergillus tubingensis S1120 with red ginseng. The chemical structures of compounds were characterized by using spectroscopic methods, the calculated and experimental electronic circular dichroism. Panaxytriol (2) from red ginseng, and asperic acid (4) showed significant antifeedant effect with the antifeedant rates of 75 % and 80 % at the concentrations of 50â µg/cm2 . Monomeric carviolin (3) and asperazine (5) displayed weak attractant activity on silkworm. All compounds were assayed for antifungal activities against phytopathogens A.â tubingensis, Nigrospora oryzae and Phoma herbarum and the results indicated that autotoxic aspertubin A (1) and panaxytriol (2) possessed selective inhibition against A.â tubingensis with MIC values at 8â µg/mL. The co-culture extract showed higher antifeedant and antifungal activities against P.â herbarum than those of monoculture of A.â tubingensis in ordinary medium. So the medicinal plant and endophyte showed synergistic effect on the plant disease resistance by active compounds from the coculture of A.â tubingensis S1120 and red ginseng.
Subject(s)
Antifungal Agents/chemistry , Aspergillus/chemistry , Insect Repellents/chemistry , Panax/chemistry , Animals , Antifungal Agents/isolation & purification , Antifungal Agents/pharmacology , Ascomycota/drug effects , Aspergillus/growth & development , Aspergillus/metabolism , Bombyx/drug effects , Bombyx/growth & development , Enediynes/chemistry , Enediynes/isolation & purification , Enediynes/pharmacology , Fatty Alcohols/chemistry , Fatty Alcohols/isolation & purification , Fatty Alcohols/pharmacology , Insect Repellents/isolation & purification , Insect Repellents/pharmacology , Microbial Sensitivity Tests , Molecular Conformation , Panax/growth & development , Panax/metabolism , Phoma/drug effects , Plants, Medicinal/chemistry , Plants, Medicinal/growth & development , Plants, Medicinal/metabolismABSTRACT
Endophytic fungi play important roles for host's stress tolerance including invasion by pathogenic microbes. Small molecules are common weapons in the microbe-microbe interactions. Panax notoginseng is a widely used traditional Chinese medicinal plant and harbors many endophytes, some exert functions against pathogens. Here, we report six new compounds named myrothins A-F (1-6) produced by Myrothecium sp. BS-31, an endophyte isolated from P. notoginseng, and their antifungal activities against pathogenic fungi causing host root-rot disease. Their structures were elucidated with analysis of spectroscopic data including 1D and 2D NMR, HR-ESI-MS. Myrothins B (2) and E (5) showed the weak activity against Fusarium oxysporum and Phoma herbarum, and myrothins F (6) showed weak activity against F. oxysporum.
