ABSTRACT
The effect of microchip pulsed electric field (MPEF) treatment on lethal and sublethal injury of Pichia rhodanensis (P. rhodanensis) were employed under 100-500 V for 20-100 pulses and the underlying mechanism of MPEF treatment was investigated as well. A 6.48 log10 reduction of P. rhodanensis was achieved at 500V for 80 pulse. The fluorescent staining with Propidium Iodide (PI) verified that the rate of sublethal injury cells maximum up to 27.2% under 200 V. MPEF can cause the damage of cell morphology and ultrastructure, meanwhile causing a decrease in cellular enzymes, antioxidant enzyme activity and cell membrane fluidity. The leakage of intracellular compounds (protein, nucleic acid, K+, Mg2+) and Ca2+-ATPase gradually increased as the growth of voltage, especially the proportion of protein in the supernatants increased from 2.0% to 26.4%. Flow cytometry analysis showed that MPEF has significant effect on membrane potential, but no obvious influence on non-specific esterase. MPEF can cause the changing of the secondary structure of protein, at the same time, double helix structure of DNA became loose and unwinding. These results provide a theoretical guidance for the widespread using of MPEF technology in the application of a non-thermal processing technique for food.
Subject(s)
Cell Membrane/ultrastructure , DNA, Fungal/chemistry , Food Preservation/instrumentation , Fungal Proteins/chemistry , Pichia/ultrastructure , Calcium-Transporting ATPases , Cell Membrane/chemistry , Electric Stimulation , Electricity , Flow Cytometry , Food Preservation/methods , Membrane Potentials , Microarray Analysis , Molecular Conformation , Pichia/physiology , Protein Structure, SecondaryABSTRACT
Carbon dots (CDs) as a new fluorescent material with excellent water solubility, chemical inertness, and easy surface modification are a good candidate for bioimaging and biosensing due to their low toxicity and good biocompatibility. Although carbon is not an intrinsically toxic substance, carbon nanomaterials such as CDs may cause risks to human health and the potentially hazardous effects of CDs on various living systems must be completely determined. So far, cytotoxicity studies of CDs have focused on human cells and are mainly conducted on limited cell lines. In the present study, toxicity assessment of CDs was evaluated on yeast cells Pichia pastoris as a unicellular eukaryotic model. Results revealed dose-dependent toxicity of CDs on yeast cells and less relative cell growth in 25â¯mg/ml of CDs as compared to the control group. CDs binding curve confirmed the interaction between CDs and surface of yeast cells. SEM images showed that the CDs caused cell shrinkage and hole formation on the surface of yeast cells and also induced slightly cell deformation. It was demonstrated that CDs could generate the ROS dose-dependently. Finally, results showed the growth inhibition and ROS generation effects of CDs were enhanced at light exposure, as an important environmental factor. These findings could have important implications for applications of CDs.
Subject(s)
Carbon , Fluorescent Dyes/toxicity , Nanostructures/toxicity , Light , Pichia/drug effects , Pichia/metabolism , Pichia/radiation effects , Pichia/ultrastructure , Reactive Oxygen Species/metabolismABSTRACT
The yeast Pichia fermentans DISAABA 726 strain (P. fermentans) is a dimorphic yeast that under different environmental conditions may switch from a yeast-like to pseudohyphal morphology. We hypothesize that exosomes-like vesicles (EV) could mediate this rapid modification. EV are membrane-derived vesicles carrying lipids, proteins, mRNAs and microRNAs and have been recognized as important mediators of intercellular communication. Although it has been assumed for a long time that fungi release EV, knowledge of their functions is still limited. In this work we analyze P. fermentans EV production during growth in two different media containing urea (YCU) or methionine (YCM) where yeast-like or pseudohyphal morphology are produced. We developed a procedure to extract EV from the neighboring biofilm which is faster and more efficient as compared to the widely used ultracentrifugation method. Differences in morphology and RNA content of EV suggest that they might have an active role during dimorphic transition as response to the growth conditions. Our findings are coherent with a general state of hypoxic stress of the pseudohyphal cells.
Subject(s)
Biofilms , Extracellular Vesicles/metabolism , Pichia/physiology , Culture Media , Extracellular Vesicles/ultrastructure , Fungal Proteins/metabolism , MicroRNAs/metabolism , Microbial Viability , Pichia/cytology , Pichia/ultrastructure , RNA, Fungal/metabolismABSTRACT
Glycosylphosphatidylinositol (GPI)-anchored glycoproteins have diverse intrinsic functions in yeasts, and they also have different uses in vitro. The GPI-modified cell wall proteins GCW21, GCW51, and GCW61 of Pichia pastoris were chosen as anchoring proteins to construct co-expression strains in P. pastoris GS115. The hydrolytic activity and the amount of Candida antarctica lipase B (CALB) displayed on cell surface increased significantly following optimization of the fusion gene dosage and combination of the homogeneous or heterogeneous cell wall proteins. Maximum CALB hydrolytic activity was achieved at 4920 U/g dry cell weight in strain GS115/CALB-GCW (51 + 51 + 61 + 61) after 120 h of methanol induction. Changes in structural morphology and the properties of the cell surfaces caused by co-expression of fusion proteins were observed by transmission electron microscopy (TEM) and on plates containing cell-wall-destabilizing reagent. Our results suggested that both the outer and inner cell layers were significantly altered by overexpression of GPI-modified cell wall proteins. Interestingly, quantitative analysis of the inner layer components showed an increase in ß-1,3-glucan, but no obvious changes in chitin in the strains overexpressing GPI-modified cell wall proteins.
Subject(s)
Cell Wall/metabolism , GPI-Linked Proteins/chemistry , GPI-Linked Proteins/metabolism , Glycosylphosphatidylinositols/metabolism , Pichia/genetics , Pichia/metabolism , Cell Wall/chemistry , Cell Wall/ultrastructure , Fungal Proteins/metabolism , GPI-Linked Proteins/genetics , Glycoproteins/chemistry , Glycoproteins/genetics , Glycoproteins/metabolism , Hydrolysis , Lipase/metabolism , Methanol/metabolism , Pichia/chemistry , Pichia/ultrastructure , beta-Glucans/metabolismABSTRACT
In this study, we investigated the classical fermentation process in Pichia pastoris based on transcriptomics. We utilized methanol in pichia yeast cell as the focus of our study, based on two key steps: limiting carbon source replacement (from glycerol to methonal) and fermentative production of exogenous proteins. In the former, the core differential genes in co-expression net point to initiation of aerobic metabolism and generation of peroxisome. The transmission electron microscope (TEM) results showed that yeast gradually adapted methanol induction to increased cell volume, and decreased density, via large number of peroxisomes. In the fermentative production of exogenous proteins, the Gene Ontology (GO) mapping results show that PAS_chr2-1_0582 played a vital role in regulating aerobic metabolic drift. In order to confirm the above results, we disrupted PAS_chr2-1_0582 by homologous recombination. Alcohol consumption was equivalent to one fifth of the normal control, and fewer peroxisomes were observed in Δ0582 strain following methanol induction. In this study we determined the important core genes and GO terms regulating aerobic metabolic drift in Pichia, as well as developing new perspectives for the continued development within this field.
Subject(s)
Pichia/metabolism , Aerobiosis/physiology , Cell Size , DNA, Fungal/genetics , Fermentation , Gene Expression Profiling , Gene Expression Regulation , Methanol/metabolism , Microscopy, Electron, Transmission , Oligonucleotide Array Sequence Analysis , Peroxisomes/metabolism , Pichia/genetics , Pichia/ultrastructure , RNA, Fungal/geneticsABSTRACT
The capability of yeast to adsorb patulin in fruit juice can aid in substantially reducing the patulin toxic effect on human health. This study aimed to investigate the capability of yeast cell morphology and cell wall internal structure and composition to adsorb patulin. To compare different yeast cell morphologies, cell wall internal structure and composition, scanning electron microscope, transmission electron microscope and ion chromatography were used. The results indicated that patulin adsorption capability of yeast was influenced by cell surface areas, volume, and cell wall thickness, as well as 1,3-ß-glucan content. Among these factors, cell wall thickness and 1,3-ß-glucan content serve significant functions. The investigation revealed that patulin adsorption capability was mainly affected by the three-dimensional network structure of the cell wall composed of 1,3-ß-glucan. Finally, patulin adsorption in commercial kiwi fruit juice was investigated, and the results indicated that yeast cells could adsorb patulin from commercial kiwi fruit juice efficiently. This study can potentially simulate in vitro cell walls to enhance patulin adsorption capability and successfully apply to fruit juice industry.
Subject(s)
Candida tropicalis/chemistry , Cell Wall/chemistry , Patulin/metabolism , Pichia/chemistry , Saccharomyces cerevisiae/chemistry , Adsorption , Candida tropicalis/metabolism , Candida tropicalis/ultrastructure , Cell Size , Cell Wall/metabolism , Cell Wall/ultrastructure , Fungal Polysaccharides/analysis , Microscopy, Electron, Scanning , Pichia/metabolism , Pichia/ultrastructure , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae/ultrastructureABSTRACT
The activity of the potent but highly toxic antifungal drug Amphotericin B (AmB), used intravenously to treat systemic fungal and parasitic infections, is widely accepted to result from its specific interaction with the fungal sterol ergosterol. While the effect of sterols on AmB activity has been intensely investigated, the role of membrane phospholipid composition has largely been ignored, and structural studies of native membranes have been hampered by their complex and disordered nature. We show for the first time that the structure of fungal membranes derived from Pichia pastoris yeast depends on the degree of lipid polyunsaturation, which has an impact on the structural consequences of AmB activity. AmB inserts in yeast membranes even in the absence of ergosterol, and forms an extra-membraneous layer whose thickness is resolved to be 4-5 nm. In ergosterol-containing membranes, AmB insertion is accompanied by ergosterol extraction into this layer. The AmB-sponge mediated depletion of ergosterol from P. pastoris membranes gives rise to a significant membrane thinning effect that depends on the degree of lipid polyunsaturation. The resulting hydrophobic mismatch is likely to interfere with a much broader range of membrane protein functions than those directly involving ergosterol, and suggests that polyunsaturated lipids could boost the efficiency of AmB. Furthermore, a low degree of lipid polyunsaturation leads to least AmB insertion and may protect host cells against the toxic effects of AmB. These results provide a new framework based on lipid composition and membrane structure through which we can understand its antifungal action and develop better treatments.
Subject(s)
Amphotericin B/chemistry , Cell Membrane/chemistry , Fatty Acids, Unsaturated/chemistry , Lipid Bilayers/chemistry , Membrane Fluidity , Pichia/chemistry , Pichia/ultrastructureABSTRACT
In this study, a novel strain of Pichia jadinii, HBY61, capable of the biocatalysis of 4-hydroxy-2-butanone (4H2B) to (R)-1,3-BD was isolated. HBY61 produced (R)-1,3-BD with high activity and absolute stereochemical selectivity (100 % e.e). Glucose and beef extract were found to be the key factors governing the fermentation, and their optimal concentrations were determined to be 84.2 and 43.7 g/L, respectively. The optimal bioconversion conditions of 4H2B catalyzed by HBY61 were pH 7.4, 30 °C, and 250 rpm with 6 % (v/v) glucose as the co-substrate. Accordingly, when 45 g/L of 4H2B was divided into three equal parts and added successively into the system at set time intervals, the maximum (R)-1,3-BD concentration reached 38.3 g/L with high yield (85.1 %) and strict 100 % enantioselectivity. Compared with previously reported yields for the biocatalytic production of (R)-1,3-BD, the use of strain HBY61 provided a high yield with excellent stereoselectivity.
Subject(s)
Butanones/metabolism , Butylene Glycols/metabolism , Pichia/metabolism , Biocatalysis , Butylene Glycols/chemistry , Fermentation , Pichia/classification , Pichia/isolation & purification , Pichia/ultrastructure , StereoisomerismABSTRACT
We demonstrate that the peroxin Pex3 is not required for the formation of peroxisomal membrane structures in yeast pex3 mutant cells. Notably, pex3 mutant cells already contain reticular and vesicular structures that harbor key proteins of the peroxisomal receptor docking complex-Pex13 and Pex14-as well as the matrix proteins Pex8 and alcohol oxidase. Other peroxisomal membrane proteins in these cells are unstable and transiently localized to the cytosol (Pex10, Pmp47) or endoplasmic reticulum (Pex11). These reticular and vesicular structures are more abundant in cells of a pex3 atg1 double deletion strain, as the absence of Pex3 may render them susceptible to autophagic degradation, which is blocked in this double mutant. Contrary to earlier suggestions, peroxisomes are not formed de novo from the endoplasmic reticulum when the PEX3 gene is reintroduced in pex3 cells. Instead, we find that reintroduced Pex3 sorts to the preperoxisomal structures in pex3 cells, after which these structures mature into normal peroxisomes.
Subject(s)
Fungal Proteins/physiology , Membrane Proteins/physiology , Peroxisomes/metabolism , Pichia/ultrastructure , Autophagy , Fungal Proteins/analysis , Fungal Proteins/genetics , Gene Deletion , Green Fluorescent Proteins/analysis , Intracellular Membranes/metabolism , Intracellular Membranes/ultrastructure , Membrane Proteins/analysis , Membrane Proteins/genetics , Membrane Transport Proteins/analysis , Membrane Transport Proteins/genetics , Membrane Transport Proteins/metabolism , Microscopy, Electron, Transmission , Peroxisomes/ultrastructure , Pichia/genetics , Pichia/metabolism , Ubiquitin-Protein Ligases/geneticsABSTRACT
The methylotrophic yeast Pichia pastoris is a popular yeast expression system for the production of heterologous proteins in biotechnology. Interestingly, cell organelles which play an important role in this process have so far been insufficiently investigated. For this reason, we started a systematic approach to isolate and characterize organelles from P. pastoris. In this study, we present a procedure to isolate microsomal membranes at high purity. These samples represent endoplasmic reticulum (ER) fractions which were subjected to molecular analysis of lipids and proteins. Organelle lipidomics included a detailed analysis of glycerophospholipids, fatty acids, sterols and sphingolipids. The microsomal proteome analyzed by mass spectrometry identified typical proteins of the ER known from other cell types, especially Saccharomyces cerevisiae, but also a number of unassigned gene products. The lipidome and proteome analysis of P. pastoris microsomes are prerequisite for a better understanding of functions of this organelle and for modifying this compartment for biotechnological applications.
Subject(s)
Fungal Proteins/analysis , Lipids/analysis , Microsomes/chemistry , Pichia/ultrastructure , Proteome/analysis , Glycerophospholipids/analysis , Pichia/chemistry , Quality Control , Sphingolipids/analysisABSTRACT
We demonstrated that in the yeast Hansenula polymorpha peroxisome fission and degradation are coupled processes that are important to remove intra-organellar protein aggregates. Protein aggregates were formed in peroxisomes upon synthesis of a mutant catalase variant. We showed that the introduction of these aggregates in the peroxisomal lumen had physiological disadvantages as it affected growth and caused enhanced levels of reactive oxygen species. Formation of the protein aggregates was followed by asymmetric peroxisome fission to separate the aggregate from the mother organelle. Subsequently, these small, protein aggregate-containing organelles were degraded by autophagy. In line with this observation we showed that the degradation of the protein aggregates was strongly reduced in dnm1 and pex11 cells in which peroxisome fission is reduced. Moreover, this process was dependent on Atg1 and Atg11.
Subject(s)
Autophagy , Fungal Proteins/chemistry , Fungal Proteins/metabolism , Peroxisomes/metabolism , Pichia/cytology , Pichia/metabolism , Proteolysis , Catalase/metabolism , Green Fluorescent Proteins/metabolism , Mutation , Oxidative Stress , Peroxisomes/ultrastructure , Pichia/growth & development , Pichia/ultrastructure , Protein Structure, Quaternary , Reactive Oxygen Species/metabolism , Saccharomyces cerevisiae/cytology , Saccharomyces cerevisiae/metabolismABSTRACT
Peroxisome division is regulated by the conserved peroxin Pex11p. In Saccharomyces cerevisiae (Sc), induction of the phosphoprotein ScPex11p coincides with peroxisome biogenesis. We show that the ScPex11p homologue in Pichia pastoris (PpPex11p) is phosphorylated at serine 173. PpPex11p expression and phosphorylation are induced in oleate and coordinated with peroxisome biogenesis. PpPex11p transits to peroxisomes via the endoplasmic reticulum (ER). PpPex11p is unstable and ER restricted gin pex3Δ and pex19Δ cells, which are impaired in peroxisomal membrane protein biogenesis. In oleate medium, the P. pastoris mutants pex11A (constitutively unphosphorylated; S173A) and pex11D (constitutively phosphorylated; S173D) exhibit juxtaposed elongated peroxisomes (JEPs) and hyperdivided forms, respectively, although protein levels remain unchanged. In contrast with ScPex11p, the ER-to-peroxisome translocation in P. pastoris is phosphorylation independent, and the phosphorylation occurs at the peroxisome. We show that PpPex11p interacts with the peroxisome fission machinery via PpFis1p and is regulated by phosphorylation because PpPex11p and PpPex11Dp interact more strongly with PpFis1p than PpPex11Ap. Neither PpPex11p nor PpFis1p is necessary for peroxisome division in methanol medium. We propose a model for the role of PpPex11p in the regulation of peroxisome division through a phosphorylation-dependent interaction with the fission machinery, providing novel insights into peroxisome morphogenesis.
Subject(s)
Fungal Proteins/metabolism , Peroxisomes/metabolism , Pichia/metabolism , Cloning, Molecular , Endoplasmic Reticulum/metabolism , Fungal Proteins/genetics , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mitochondrial Proteins/genetics , Mitochondrial Proteins/metabolism , Models, Biological , Molecular Sequence Data , Mutant Proteins/genetics , Mutant Proteins/metabolism , Oleic Acid/metabolism , Peroxins , Peroxisomes/ultrastructure , Phosphorylation , Pichia/genetics , Pichia/ultrastructure , Protein Transport , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/metabolism , Saccharomyces cerevisiae Proteins/genetics , Saccharomyces cerevisiae Proteins/metabolism , Species SpecificityABSTRACT
In this study, simultaneous saccharification and fermentation (SSF) was employed to produce ethanol from 1% sodium hydroxide-treated rice straw in a thermostatically controlled glass reactor using 20 FPU gds⻹ cellulase, 50 IU gds⻹ ß-glucosidase, 15 IU gds⻹ pectinase and a newly isolated thermotolerant Pichia kudriavzevii HOP-1 strain. Scanning electron micrograph images showed that the size of the P. kudriavzevii cells ranged from 2.48 to 6.93 µm in diameter while the shape of the cells varied from oval, ellipsoidal to elongate. Pichia kudriavzevii cells showed extensive pseudohyphae formation after 5 days of growth and could assimilate sugars like glucose, sucrose, galactose, fructose, and mannose but the cells could not assimilate xylose, arabinose, cellobiose, raffinose, or trehalose. In addition, the yeast cells could tolerate up to 40% glucose and 5% NaCl concentrations but their growth was inhibited at 1% acetic acid and 0.01% cyclohexamide concentrations. Pichia kudriavzevii produced about 35 and 200% more ethanol than the conventional Saccharomyces cerevisiae cells at 40 and 45°C, respectively. About 94% glucan in alkali-treated rice straw was converted to glucose through enzymatic hydrolysis within 36 h. Ethanol concentration of 24.25 g l⻹ corresponding to 82% theoretical yield on glucan basis and ethanol productivity of 1.10 g l⻹ h⻹ achieved using P. kudriavzevii during SSF hold promise for scale-up studies. An insignificant amount of glycerol and no xylitol was produced during SSF. To the best of our knowledge, this is the first study reporting ethanol production from any lignocellulosic biomass using P. kudriavzevii.
Subject(s)
Biofuels , Ethanol/metabolism , Industrial Microbiology , Oryza/metabolism , Pichia/physiology , Cellulase/metabolism , Fermentation , Hydrolysis , Pichia/isolation & purification , Pichia/ultrastructure , Saccharomyces cerevisiae/metabolism , beta-Glucosidase/metabolismABSTRACT
Evidence is accumulating that damaged components of eukaryotic cells are removed by autophagic degradation (e.g., mitophagy). Here we show that peroxisomes that are damaged by the abrupt removal of the membrane protein Pex3 are massively and rapidly degraded even when the cells are placed at peroxisome-inducing conditions and hence need the organelles for growth. Pex3 degradation was induced by a temperature shift using Hansenula polymorpha pex3Δ cells producing a Pex3 fusion protein containing an N-terminal temperature sensitive degron sequence. The massive peroxisome degradation process, associated with Pex3 degradation, showed properties of both micro- and macropexophagy and was dependent on Atg1 and Ypt7. This mode of peroxisome degradation is of physiological significance as it was also observed at conditions that excessive ROS is formed from peroxisome metabolism, i.e., when methanol-grown wild-type cells are exposed to methanol excess conditions.
Subject(s)
Autophagy , Peroxisomes/metabolism , Pichia/cytology , Pichia/metabolism , Autophagy/drug effects , Cycloheximide/pharmacology , Fungal Proteins/metabolism , Methanol/pharmacology , Microscopy, Fluorescence , Peroxisomes/drug effects , Peroxisomes/ultrastructure , Pichia/drug effects , Pichia/ultrastructure , Protein Processing, Post-Translational/drug effects , Protein Transport/drug effectsABSTRACT
Membrane remodeling is an important aspect in organelle biogenesis. We show that different peroxisome membrane proteins that play a role in organelle biogenesis and proliferation (Pex8, Pex10, Pex14, Pex25 and Pex11) are subject to spatiotemporal behavior during organelle development. Using fluorescence microscopy analysis of Hansenula polymorpha dnm1 cells that are blocked in the normal fission process, we show that green fluorescent protein (GFP) fusions of Pex8, Pex10, Pex14 and Pex25 show enhanced fluorescence at the organelle extensions that are formed in budding cells. In contrast, Pex11 fluorescence is enriched at the base of this extension on the mother organelle. A fusion protein of GFP with the transporter Pmp47, used as a control, did not show enhanced fluorescence at any specific region of the organelle. The concentration of specific peroxins at the peroxisome surface was lost upon deletion of PEX11 or the N-terminal domain of Pex11 that is involved in membrane remodeling. Comparable distribution patterns as in dnm1 cells were observed in wild-type cells where Pex8, Pex10, Pex14 and Pex25, but not Pex11, were especially present at newly formed organelles that migrated to the bud. We speculate that peroxin reorganization events result in enhanced levels of peroxins involved in peroxisome biogenesis in nascent organelles.
Subject(s)
Fungal Proteins/metabolism , Membrane Fusion/physiology , Membrane Proteins/metabolism , Peroxisomes/metabolism , Fungal Proteins/genetics , Membrane Proteins/genetics , Models, Biological , Peroxisomes/chemistry , Pichia/genetics , Pichia/metabolism , Pichia/ultrastructure , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolismABSTRACT
Variações genética e antigênica são observadas com frequência elevada entre estirpes do VBIG e envolvem principalmente a glicoproteína S1. Com o objetivo de contribuir com a disponibilidade de ferramentas para o imunodiagnóstico e a imunoprofilaxia da bronquite infecciosa das galinhas foi desenvolvida uma metodologia para expressão recombinante da glicoproteína S1 na levedura Picchia pastoris. O cDNA do gene codificador dessa proteína foi obtido a partir de RNA viral de ovos embrionados infectados com a estirpe M41 do VBIG submetido à transcrição reversa (RT) e reação em cadeia da polimerase (PCR), amplificando-se a sequência codificadora de S1 acrescida de extremidades compatíveis com a clonagem no vetor usado na transformação de leveduras. A indução com metanol resultou na produção de uma proteína detectada como banda única do tamanho previsto, em western-blot, no lisado celular das leveduras transformadas. A expressão em P. pastoris mostrou ser um método eficaz para a produção recombinante da proteína S1 do VBIG, com potencial para utilização em técnicas de imunodiagnóstico da bronquite infecciosa das galinhas.
Genetic and antigenic variation are very frequently observed among IBV strains and affect mainly the S1 glycoprotein. In order to contribute to the availability of tools for immunodiagnosis and immunoprophylaxis of chicken infectious bronchitis we developed an expression system for production of recombinant S1 glycoprotein in Pichia pastoris. We obtained the cDNA from viral RNA on embryonated eggs infected with the M41 strain of IBV, by reverse transcription (RT) and polymerase chain reaction (PCR), amplifying the S1 coding sequence with extremities compatible with the vector used to transform yeast. Induction with methanol led to the production of a protein with the predicted molecular weight that was detected by Western blot in the cell lysate of transformed yeast. Expression in P. pastoris proved to be an effective method for recombinant production of S1 protein from IBV, with potential for use in immuno-diagnosis of chicken infectious bronchitis virus.
Subject(s)
Animals , Pichia/ultrastructure , Glycoproteins/analysis , Chickens/virology , Viral Fusion Proteins/analysis , Infectious bronchitis virus/geneticsABSTRACT
BACKGROUND: Methylotrophic yeast species (e.g. Hansenula polymorpha, Pichia pastoris) can grow on methanol as sole source of carbon and energy. These organisms are important cell factories for the production of recombinant proteins, but are also used in fundamental research as model organisms to study peroxisome biology. During exponential growth on glucose, cells of H. polymorpha typically contain a single, small peroxisome that is redundant for growth while on methanol multiple, enlarged peroxisomes are present. These organelles are crucial to support growth on methanol, as they contain key enzymes of methanol metabolism.In this study, changes in the transcriptional profiles during adaptation of H. polymorpha cells from glucose- to methanol-containing media were investigated using DNA-microarray analyses. RESULTS: Two hours after the shift of cells from glucose to methanol nearly 20% (1184 genes) of the approximately 6000 annotated H. polymorpha genes were significantly upregulated with at least a two-fold differential expression. Highest upregulation (> 300-fold) was observed for the genes encoding the transcription factor Mpp1 and formate dehydrogenase, an enzyme of the methanol dissimilation pathway. Upregulated genes also included genes encoding other enzymes of methanol metabolism as well as of peroxisomal beta-oxidation.A moderate increase in transcriptional levels (up to 4-fold) was observed for several PEX genes, which are involved in peroxisome biogenesis. Only PEX11 and PEX32 were higher upregulated. In addition, an increase was observed in expression of the several ATG genes, which encode proteins involved in autophagy and autophagy processes. The strongest upregulation was observed for ATG8 and ATG11.Approximately 20% (1246 genes) of the genes were downregulated. These included glycolytic genes as well as genes involved in transcription and translation. CONCLUSION: Transcriptional profiling of H. polymorpha cells shifted from glucose to methanol showed the expected downregulation of glycolytic genes together with upregulation of the methanol utilisation pathway. This serves as a confirmation and validation of the array data obtained. Consistent with this, also various PEX genes were upregulated. The strong upregulation of ATG genes is possibly due to induction of autophagy processes related to remodeling of the cell architecture required to support growth on methanol. These processes may also be responsible for the enhanced peroxisomal beta-oxidation, as autophagy leads to recycling of membrane lipids. The prominent downregulation of transcription and translation may be explained by the reduced growth rate on methanol (td glucose 1 h vs td methanol 4.5 h).
Subject(s)
Adaptation, Physiological/genetics , Gene Expression Profiling , Methanol/metabolism , Pichia/genetics , Autophagy , DNA, Fungal/genetics , Fatty Acids/metabolism , Gene Expression Regulation, Fungal , Gene Regulatory Networks , Glucose/metabolism , Metabolic Networks and Pathways , Mitochondria/metabolism , Oligonucleotide Array Sequence Analysis , Pichia/metabolism , Pichia/ultrastructure , Reactive Oxygen Species/metabolismABSTRACT
This study investigated the effectiveness of heat treatment and Pichia guilliermondii, either alone or in combination, to combat postharvest fungal spoilage in cherry tomato fruit. In vitro experiments demonstrated that heat treatment at 38 degrees C significantly inhibited mycelial growth of three different pathogens (Botrytis cinerea, Alternaria alternata and Rhizopus stolonifer Ehrenb). In vivo experiments unveiled that either heat treatment or P. guilliermondii reduced decay caused by these pathogens. Furthermore, a combination of heat treatment followed by the application of P. guilliermondii (H+P) provided the best efficacy in prevention of cherry tomato from fungal spoilage. Following, H+P treatment, electronic nose detected a reduction of volatility in cherry tomato fruit odor, an indicator of preserving fruit's freshness. Scanning electron microscopy unveiled that heat treatment at 38 degrees C for 24h inhibited hyphae growth and spore germination of R. stolonifer Ehrenb while P. guilliermondii multiplied rapidly on fruit wounds, and its cells had a strong capability of adhesion to the hyphae of R. stolonifer Ehrenb. However, heat treatment also seriously injured P. guilliermondii, therefore P. guilliermondii should be applied after heat treatment. A combination of heat treatment and P. guilliermondii is one of the most effective techniques at controlling postharvest fungal spoilage in cherry tomato fruit.
Subject(s)
Food Handling/methods , Food Microbiology , Pichia/physiology , Solanum lycopersicum/microbiology , Alternaria/growth & development , Botrytis/growth & development , Hot Temperature , Microscopy, Electron, Scanning , Odorants , Pichia/growth & development , Pichia/ultrastructure , Rhizopus/growth & development , Rhizopus/ultrastructureABSTRACT
Cell-surface expression of phytase allows the enzyme to be expressed and anchored on the cell surface of Pichia pastoris. This avoids tedious downstream processes such as purification and separation involved with extracellular expression. In addition, yeast cells with anchored proteins can be used as a whole-cell biocatalyst with high value added. In this work, the phytase was expressed on the cell surface of P. pastoris with a glycosylphosphatidylinositol anchoring system. The recombinant phytase was shown to be located at the cell surface. The cell-surface phytase exhibited high activity with an optimal temperature at 50-55 degrees C and two optimal pH peaks of 3 and 5.5. The surface-displayed phytase also exhibited similar pH stability and pepsin resistance to the native and secreted phytase. In vitro digestibility test showed that P. pastoris containing cell-surface phytase released phosphorus from feedstuff at a level similar to secreted phytase. Yeast cells expressing phytase also provide additional nutrients, especially biotin and niacin. Thus, P. pastoris with phytase displayed on its surface has a great potential as a whole-cell supplement to animal feed.
Subject(s)
6-Phytase/metabolism , Cell Wall/enzymology , Pichia/enzymology , 6-Phytase/genetics , Aldehyde Oxidase/genetics , Animal Feed , Animals , Dietary Supplements , Glycosylphosphatidylinositols/metabolism , Hydrogen-Ion Concentration , Mating Factor , Peptides/genetics , Peptides/metabolism , Phosphorus, Dietary/metabolism , Pichia/ultrastructure , Promoter Regions, Genetic , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Substrate Specificity , TemperatureABSTRACT
This study aimed at developing an alternative host for the production of penicillin (PEN). As yet, the industrial production of this beta-lactam antibiotic is confined to the filamentous fungus Penicillium chrysogenum. As such, the yeast Hansenula polymorpha, a recognized producer of pharmaceuticals, represents an attractive alternative. Introduction of the P. chrysogenum gene encoding the non-ribosomal peptide synthetase (NRPS) delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS) in H. polymorpha, resulted in the production of active ACVS enzyme, when co-expressed with the Bacillus subtilis sfp gene encoding a phosphopantetheinyl transferase that activated ACVS. This represents the first example of the functional expression of a non-ribosomal peptide synthetase in yeast. Co-expression with the P. chrysogenum genes encoding the cytosolic enzyme isopenicillin N synthase as well as the two peroxisomal enzymes isopenicillin N acyl transferase (IAT) and phenylacetyl CoA ligase (PCL) resulted in production of biologically active PEN, which was efficiently secreted. The amount of secreted PEN was similar to that produced by the original P. chrysogenum NRRL1951 strain (approx. 1 mg/L). PEN production was decreased over two-fold in a yeast strain lacking peroxisomes, indicating that the peroxisomal localization of IAT and PCL is important for efficient PEN production. The breakthroughs of this work enable exploration of new yeast-based cell factories for the production of (novel) beta-lactam antibiotics as well as other natural and semi-synthetic peptides (e.g. immunosuppressive and cytostatic agents), whose production involves NRPS's.