ABSTRACT
Pinacidil, a nonselective ATP-sensitive K+ (KATP) channel opener, has cardioprotective effects for hypertension, ischemia/reperfusion injury, and arrhythmia. This agent abolishes early afterdepolarizations, delayed afterdepolarizations (DADs), and abnormal automaticity in canine cardiac ventricular myocytes. DADs are well known to be caused by the Na+/Ca2+ exchange current (INCX). In this study, we used the whole-cell patch-clamp technique and Fura-2/AM (Ca2+-indicator) method to investigate the effect of pinacidil on INCX in isolated guinea pig cardiac ventricular myocytes. In the patch-clamp study, pinacidil enhanced INCX in a concentration-dependent manner. The half-maximal effective concentration values were 23.5 and 23.0 µM for the Ca2+ entry (outward) and Ca2+ exit (inward) components of INCX, respectively. The pinacidil-induced INCX increase was blocked by L-NAME, a nitric oxide (NO) synthase inhibitor, by ODQ, a soluble guanylate cyclase inhibitor, and by KT5823, a cyclic guanosine monophosphate (cGMP)-dependent protein kinase (PKG) inhibitor, but not by N-2-mercaptopropyonyl glycine (MPG), a reactive oxygen species (ROS) scavenger. Glibenclamide, a nonselective KATP channel inhibitor, blocked the pinacidil-induced INCX increase, while 5-HD, a selective mitochondria KATP channel inhibitor, did not. In the Fura-2/AM study pinacidil also enhanced intracellular Ca2+ concentration, which was inhibited by L-NAME, ODQ, KT5823, and glibenclamide, but not by MPG and 5-HD. Sildenafil, a phosphodiesterase 5 inhibitor, increased further the pinacidil-induced INCX increase. Sodium nitroprusside, a NO donor, also increased INCX. In conclusion, pinacidil may stimulate cardiac Na+/Ca2+ exchanger (NCX1) by opening plasma membrane KATP channels and activating the NO/cGMP/PKG signaling pathway.
Subject(s)
Cyclic GMP-Dependent Protein Kinases , Cyclic GMP , KATP Channels/agonists , Myocytes, Cardiac/drug effects , Nitric Oxide , Pinacidil/pharmacology , Signal Transduction/drug effects , Sodium-Calcium Exchanger/metabolism , Animals , Antioxidants/pharmacology , Guinea Pigs , Heart Ventricles/cytology , Heart Ventricles/metabolism , Myocytes, Cardiac/metabolism , Patch-Clamp Techniques , Pinacidil/antagonists & inhibitors , Stimulation, ChemicalABSTRACT
Short QT syndrome (SQTS) is a genetic arrhythmogenic disease that can cause malignant arrhythmia and sudden cardiac death. The current therapies for SQTS have application restrictions. We previously found that Mg· (NH2CH2CH2SO3)2· H2O, a taurine-magnesium coordination compound (TMCC) exerted anti-arrhythmic effects with low toxicity. In this study we established 3 different models to assess the potential anti-arrhythmic effects of TMCC on type 2 short QT syndrome (SQT2). In Langendorff guinea pig-perfused hearts, perfusion of pinacidil (20 µmol/L) significantly shortened the QT interval and QTpeak and increased rTp-Te (P<0.05 vs control). Subsequently, perfusion of TMCC (1-4 mmol/L) dose-dependently increased the QT interval and QTpeak (P<0.01 vs pinacidil). TMCC perfusion also reversed the rTp-Te value to the normal range. In guinea pig ventricular myocytes, perfusion of trapidil (1 mmol/L) significantly shortened the action potential duration at 50% (APD50) and 90% repolarization (APD90), which was significantly reversed by TMCC (0.01-1 mmol/L, P<0.05 vs trapidil). In HEK293 cells that stably expressed the outward delayed rectifier potassium channels (IKs), perfusion of TMCC (0.01-1 mmol/L) dose-dependently inhibited the IKs current with an IC50 value of 201.1 µmol/L. The present study provides evidence that TMCC can extend the repolarization period and inhibit the repolarizing current, IKs, thereby representing a therapeutic candidate for ventricular arrhythmia in SQT2.
Subject(s)
Arrhythmias, Cardiac/prevention & control , Coordination Complexes/pharmacology , Heart Conduction System/abnormalities , Heart Defects, Congenital/prevention & control , Magnesium/pharmacology , Taurine/pharmacology , Action Potentials/drug effects , Animals , Arrhythmias, Cardiac/chemically induced , Cells, Cultured , Guinea Pigs , Heart Defects, Congenital/chemically induced , Humans , Magnesium/chemistry , Models, Theoretical , Myocytes, Cardiac/physiology , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Taurine/chemistry , Trapidil/antagonists & inhibitors , Trapidil/pharmacologyABSTRACT
K(ATP) channels are composed of sulphonylurea receptors (SURs) and potassium inward rectifiers (Kir(6.x)) that assemble to form a large octameric channel. This study was designed to examine the expression and role of sulphonylurea-binding regulatory subunits 1 [SUR1 (ABCC8)] and 2 [SUR2 (ABCC9)] of the K(ATP) channels in the pregnant rat myometrium with particular regard to the contractility. RT-PCR and Western blot analysis were performed to detect the presence of SUR1 and SUR2. The SUR1 levels were markedly increased in the early stages of pregnancy. The highest level was detected on day 6 of pregnancy, while in the late stages the levels of SUR1 were significantly decreased. The SUR2 level remained unchanged throughout pregnancy. The SUR-non-selective diazoxide and the SUR2-selective pinacidil inhibited oxytocin-induced contractions. Glibenclamide, a K(ATP) channel blocker, antagonized both pinacidil and diazoxide-induced relaxations. It was established that SURs are responsible for pharmacological reactivity of K(ATP) channel openers. We conclude that, both SURs are involved in the K(ATP) channel in the pregnant rat myometrium. It may further be concluded that "pinacidil-like" K(ATP) channel openers may be of therapeutic relevance as tocolytic agents in the future.
Subject(s)
ATP-Binding Cassette Transporters/metabolism , KATP Channels/metabolism , Myometrium/metabolism , Oxytocin/antagonists & inhibitors , Potassium Channel Blockers/pharmacology , Potassium Channels, Inwardly Rectifying/metabolism , Receptors, Drug/metabolism , Sulfonylurea Compounds/metabolism , Tocolytic Agents/pharmacology , Uterine Contraction/drug effects , ATP-Binding Cassette Transporters/agonists , ATP-Binding Cassette Transporters/antagonists & inhibitors , ATP-Binding Cassette Transporters/genetics , Animals , Blotting, Western , Diazoxide/antagonists & inhibitors , Diazoxide/pharmacology , Female , Gene Expression Regulation , Glyburide/pharmacology , KATP Channels/agonists , KATP Channels/antagonists & inhibitors , KATP Channels/genetics , Myometrium/drug effects , Oxytocin/metabolism , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Potassium Channels, Inwardly Rectifying/agonists , Potassium Channels, Inwardly Rectifying/antagonists & inhibitors , Potassium Channels, Inwardly Rectifying/genetics , Pregnancy , Protein Isoforms/metabolism , Protein Subunits/metabolism , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Drug/agonists , Receptors, Drug/antagonists & inhibitors , Receptors, Drug/genetics , Sulfonylurea ReceptorsABSTRACT
ATP-sensitive potassium channels (K(ATP) channels) are composed of sulfonylurea receptors (SURs) and potassium inward rectifiers (Kir(6.x)) that assemble to form a large octameric channel. This study was designed to examine the expression and role of sulfonylurea-binding regulatory subunits 1 (SUR1 (ABCC8)) and 2 (SUR2 (ABCC9)) of the K(ATP) channels in the pregnant rat myometrium with particular regard to the contractility. RT-PCR and western blot analyses were performed to detect the presence of SUR1 and SUR2. The SUR1 levels were markedly increased in the early stages of pregnancy. The highest level was detected on day 6 of pregnancy, whereas in the late stages, the levels of SUR1 were significantly decreased. The SUR2 level remained unchanged throughout pregnancy. The SUR non-selective diazoxide and the SUR2-selective pinacidil inhibited oxytocin-induced contractions. Glibenclamide, a K(ATP) channel blocker, antagonized both pinacidil- and diazoxide-induced relaxations. It was established that SURs are responsible for pharmacological reactivity of K(ATP) channel openers. We conclude that both SURs are involved in the K(ATP) channel in the pregnant rat myometrium. It may further be concluded that 'pinacidil-like' K(ATP) channel openers may be of therapeutic relevance as tocolytic agents in the future.
Subject(s)
KATP Channels/metabolism , Myometrium/metabolism , Pregnancy Proteins/metabolism , Protein Subunits/metabolism , Sulfonylurea Compounds/metabolism , ATP-Binding Cassette Transporters/agonists , ATP-Binding Cassette Transporters/antagonists & inhibitors , ATP-Binding Cassette Transporters/genetics , ATP-Binding Cassette Transporters/metabolism , Animals , Diazoxide/antagonists & inhibitors , Diazoxide/pharmacology , Female , Gene Expression Regulation , In Vitro Techniques , KATP Channels/agonists , KATP Channels/antagonists & inhibitors , KATP Channels/genetics , Myometrium/drug effects , Oxytocin/antagonists & inhibitors , Oxytocin/metabolism , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Potassium Channel Blockers/pharmacology , Potassium Channels, Inwardly Rectifying/agonists , Potassium Channels, Inwardly Rectifying/antagonists & inhibitors , Potassium Channels, Inwardly Rectifying/genetics , Potassium Channels, Inwardly Rectifying/metabolism , Pregnancy , Pregnancy Proteins/agonists , Pregnancy Proteins/antagonists & inhibitors , Pregnancy Proteins/genetics , Protein Isoforms/agonists , Protein Isoforms/antagonists & inhibitors , Protein Isoforms/genetics , Protein Isoforms/metabolism , Protein Subunits/agonists , Protein Subunits/antagonists & inhibitors , Protein Subunits/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-Dawley , Receptors, Drug/agonists , Receptors, Drug/antagonists & inhibitors , Receptors, Drug/genetics , Receptors, Drug/metabolism , Sulfonylurea Receptors , Tocolytic Agents/pharmacology , Uterine Contraction/drug effectsABSTRACT
BACKGROUND: Recently, gender related differences in heart function have been extensively studied. Some of them, as differences in repolarization between males and females have been explained by direct effect of estrogen on delayed rectifier K+ channels and Ca2+ channels. It seems that estrogen induces overexpression of SUR2A subunits of ATP-sensitive K+ channels. The aim of this paper was to compare heart rate changes in male and female guinea pigs in the presence of different potassium channel openers (PCOs). METHODS: We used spontaneously beating right atria from control and estrogen receptor modulator-treated male and female guinea pigs (17-beta-estradiol as a stimulator and tamoxifen as a blocker of estrogen receptor located in heart muscle). RESULTS: In control females, rilmakalim and diazoxide, but not pinacidil elicited concentration-dependent decrease of heart rate. On the other hand, all three PCOs induced similar negative chronotropic action in hearts obtained from male control group (Emax was between -40 and -70 bpm, respectively). After two weeks of treatment with 17-beta-estradiol, pinacidil failed to significantly decrease heart rate in males however, tamoxifen-pretreated female group responded by decrease in automatism in the presence of rising concentration of pinacidil (Emax=-45+/-6 bpm, not significantly different from Emax in male control=-40+/-5 bpm, n=7). Interestingly, we observed lower blood concentration of the heart form of lactate dehydrogenase (H-LDH) in female than in male control group. Moreover, H-LDH concentration increased in tamoxifen-pretreated female group and decreased in 17-beta-estradiol-treated male group. CONCLUSION: Our results indicate that estrogen downregulates H-LDH production and specifically modulate pinacidil action in guinea pig right atria, probably by changes of binding site for this drug in SUR2A receptor, but not for rilmakalim and diazoxide.
Subject(s)
Estrogens/pharmacology , Heart Atria/drug effects , Heart Rate/drug effects , Pinacidil/antagonists & inhibitors , Vasodilator Agents/antagonists & inhibitors , Animals , Bronchodilator Agents/pharmacology , Chromans/pharmacology , Diazoxide/pharmacology , Down-Regulation , Estradiol/pharmacology , Estrogen Antagonists/pharmacology , Female , Guinea Pigs , Heart Atria/metabolism , L-Lactate Dehydrogenase/metabolism , Male , Pinacidil/pharmacology , Potassium Channels/metabolism , Pyrrolidines/pharmacology , Receptors, Estrogen/metabolism , Tamoxifen/pharmacology , Vasodilator Agents/pharmacologyABSTRACT
The purpose of this study was to investigate vascular preconditioning of individual microvascular networks. Prior work shows that exposure of downstream arterioles to specific agonists preconditions upstream arterioles so that they exhibit an altered local vasoactive response [remote microvascular preconditioning (RMP)]. We hypothesized that mitochondrial ATP-sensitive K+ (K(ATP)) channels were involved in stimulation of RMP. Arteriolar diameter (approximately 15 microm) was observed approximately 1,000 microm upstream of the remote exposure site in the cheek pouch of pentobarbital sodium-anesthetized (70 mg/kg) male hamsters (n = 104); all agonists were applied via micropipette. RMP was initiated by application of pinacidil (Pin), diazoxide (DZ), sodium nitroprusside (SNP), or bradykinin (BK) to the downstream vessel. After 15 min, RMP was apparent at the upstream observation site from testing of local vasoactive responses to L-arginine. Pin, DZ, SNP, and BK each stimulated RMP. To evaluate a specific role for mitochondrial K(ATP) channels in this response, 5-hydroxydecanoate was applied (via a 2nd pipette) during downstream stimulation with agonist. 5-Hydroxydecanoate blocked RMP initiated by Pin, DZ, or SNP, suggesting that mitochondrial K(ATP) channels are involved before SNP signal transduction. To verify this, we applied N(omega)-nitro-L-arginine during DZ or SNP stimulation. RMP was blocked during SNP, but not during DZ, stimulation. Thus stimulation of the RMP response requires mitochondrial K(ATP) channel activity after stimulation by nitric oxide donors.
Subject(s)
ATP-Binding Cassette Transporters/physiology , Ischemic Preconditioning/methods , Potassium Channels, Inwardly Rectifying/physiology , ATP-Binding Cassette Transporters/agonists , ATP-Binding Cassette Transporters/drug effects , Adenosine/pharmacology , Animals , Arginine , Arterioles/drug effects , Bradykinin/pharmacology , Cricetinae , Cyclic GMP/analogs & derivatives , Cyclic GMP/pharmacology , Decanoic Acids/pharmacology , Diazoxide/antagonists & inhibitors , Diazoxide/pharmacology , Hydroxy Acids/pharmacology , KATP Channels , Male , Mesocricetus , Microcirculation , Nitric Oxide Donors/pharmacology , Nitroprusside/pharmacology , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Potassium Channels, Inwardly Rectifying/agonists , Potassium Channels, Inwardly Rectifying/drug effects , Signal Transduction/drug effects , Vasodilation/drug effectsABSTRACT
Using nystatin-perforated patch-clamp and whole cell recording, we tested the hypothesis that K(ATP) channels contribute to resting conductance of rat descending vasa recta (DVR) pericytes and are modulated by vasoconstrictors. The K(ATP) blocker glybenclamide (Glb; 10 microM) depolarized pericytes and inhibited outward currents of cells held at -40 mV. K(ATP) openers pinacidil (Pnc; 10 microM) and P-1075 (1 microM) hyperpolarized pericytes and transiently augmented outward currents. All effects of Pnc and P-1075 were fully reversed by Glb. Inward currents of pericytes held at -60 mV in symmetrical 140 mM K(+) were markedly augmented by Pnc and fully reversed by Glb. Ramp depolarizations in symmetrical K(+), performed in Pnc and Pnc + Glb, yielded a Pnc-induced, Glb-sensitive K(ATP) difference current that lacked rectification and reversed at 0 mV. Immunostaining identified both K(IR)6.1, K(IR)6.2 inward rectifier subunits and sulfonurea receptor subtype 2B. ANG II (1 and 10 nM) and endothelin-1 (10 nM) but not vasopressin (100 nM) significantly lowered holding current at -40 mV and abolished Pnc-stimulated outward currents. We conclude that DVR pericytes express K(ATP) channels that make a significant contribution to basal K(+) conductance and are inhibited by ANG II and endothelin-1.
Subject(s)
ATP-Binding Cassette Transporters/physiology , Juxtaglomerular Apparatus/physiology , Pericytes/physiology , Potassium Channels, Inwardly Rectifying/physiology , ATP-Binding Cassette Transporters/drug effects , Angiotensin II/pharmacology , Animals , Electric Capacitance , Endothelin-1/pharmacology , Glyburide/pharmacology , KATP Channels , Membrane Potentials/drug effects , Pinacidil/antagonists & inhibitors , Potassium Channels, Inwardly Rectifying/drug effects , Rats , Rats, Sprague-Dawley , Vasopressins/pharmacologyABSTRACT
1 The aim of this study was to investigate the effect of nicotinamide-adenine dinucleotide (NADH) supplementation on the metabolic condition of isolated guinea-pig ventricular cardiomyocytes. The pinacidil-primed ATP-dependent potassium current I(K(ATP)) was used as an indicator of subsarcolemmal ATP concentration and intracellular adenine nucleotide contents were measured. 2 Membrane currents were studied using the patch-clamp technique in the whole-cell recording mode at 36-37 degrees C. Adenine nucleotides were determined by HPLC. 3 Under physiological conditions (4.3 mM ATP in the pipette solution, ATP(i)) I(K(ATP)) did not contribute to basal electrical activity. 4 The ATP-dependent potassium (K((ATP))) channel opener pinacidil activated I(K(ATP)) dependent on [ATP](i) showing a significantly more pronounced activation at lower (1 mM) [ATP](i). 5 Supplementation of cardiomyocytes with 300 micro g ml(-1) NADH (4-6 h) resulted in a significantly reduced I(K(ATP)) activation by pinacidil compared to control cells. The current density was 13.8+/-3.78 (n=6) versus 28.9+/-3.38 pA pF(-1) (n=19; P<0.05). 6 Equimolar amounts of the related compounds nicotinamide and NAD(+) did not achieve a similar effect like NADH. 7 Measurement of adenine nucleotides by HPLC revealed a significant increase in intracellular ATP (NADH supplementation: 45.6+/-1.88 nmol mg(-1) protein versus control: 35.4+/-2.57 nmol mg(-1) protein, P<0.000005). 8 These data show that supplementation of guinea-pig ventricular cardiomyocytes with NADH results in a decreased activation of I(K(ATP)) by pinacidil compared to control myocytes, indicating a higher subsarcolemmal ATP concentration. 9 Analysis of intracellular adenine nucleotides by HPLC confirmed the significant increase in ATP.
Subject(s)
Adenosine Triphosphate/physiology , Myocytes, Cardiac/drug effects , NAD/physiology , NAD/pharmacokinetics , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacokinetics , Action Potentials , Animals , Dose-Response Relationship, Drug , Glyburide/pharmacokinetics , Guinea Pigs , Heart Ventricles , Myocytes, Cardiac/metabolism , Patch-Clamp Techniques , Pinacidil/administration & dosage , Potassium Channels/drug effects , Potassium Channels/physiology , Sarcolemma/chemistry , Sarcolemma/drug effects , Sarcolemma/enzymologyABSTRACT
BACKGROUND: Cardioprotective effects of isoflurane are partially mediated by the sarcolemmal adenosine triphosphate-sensitive potassium (sarcK ATP ) channel. The authors tested the hypothesis that isoflurane sensitizes sarcK ATP channels to a potassium channel opener, pinacidil, adenosine- and phospholipid-mediated pathways. METHODS: Activation by pinacidil of the K ATP current (I KATP ) was monitored in guinea pig ventricular myocytes at 0.5 and 5 mm intracellular ATP in the whole cell configuration of the patch clamp technique. The sensitization effect was evaluated by pretreating each myocyte with isoflurane (0.57 +/- 0.04 mm) before application of pinacidil (5 micro m) in the continued presence of the anesthetic. To investigate whether intracellular signaling pathways may be involved in isoflurane sensitization, the authors used the adenosine receptor antagonist theophylline (100 micro m) and the phosphatidylinositol kinase inhibitor wortmannin (100 micro m). RESULTS: The density of pinacidil-activated I KATP was higher at 0.5 mm ATP (20.7 +/- 3.2 pA/pF) than at 5 mm ATP (2.0 +/- 0.3 pA/pF). At 0.5 mm ATP, pretreatment with isoflurane caused an increase in density of pinacidil-activated I KATP (42.4 +/- 6.2 pA/pF) and accelerated the rate of current activation (from 5.4 +/- 1.2 to 39.0 +/- 7.9 pA. pF(-1). min(-1) ). Theophylline attenuated current activation by pinacidil (9.4 +/- 3.9 pA/pF) and abolished the sensitization effect of isoflurane on I KATP (10.0 +/- 2.5 pA/pF). Wortmannin did not alter pinacidil activation of I KATP (13.2 +/- 1.7 pA/pF) but prevented sensitization by isoflurane (15.8 +/- 4.5 pA/pF). CONCLUSIONS: These results suggest that isoflurane increases sensitivity of cardiac sarcK ATP channels to the potassium channel opener pinacidil. Blockade of adenosine receptors or phosphatidylinositol kinases abolishes the sensitization effect, suggesting that the adenosine and phospholipid signaling pathways may be involved in the actions by isoflurane.
Subject(s)
Anesthetics, Inhalation/pharmacology , Heart/drug effects , Isoflurane/pharmacology , Pinacidil/pharmacology , Potassium Channels/agonists , Potassium Channels/metabolism , Sarcolemma/drug effects , Sarcolemma/metabolism , Vasodilator Agents/pharmacology , ATP-Binding Cassette Transporters , Androstadienes/pharmacology , Animals , Female , Glyburide/pharmacology , Guinea Pigs , Hypoglycemic Agents/pharmacology , In Vitro Techniques , KATP Channels , Male , Membrane Potentials/drug effects , Patch-Clamp Techniques , Phospholipids/physiology , Pinacidil/antagonists & inhibitors , Potassium Channels, Inwardly Rectifying , Signal Transduction/drug effects , Vasodilator Agents/antagonists & inhibitors , WortmanninABSTRACT
Phosphatidylinositol 4,5-bisphosphate (PIP2) stimulates ATP-sensitive K+ (K(ATP)) channel activity. Because phospholipase C (PLC) hydrolyzes membrane-bound PIP2, which in turn may potentially decrease K(ATP) channel activity, we investigated the effects of the alpha1-adrenoceptor-G(q)-PLC signal transduction axis on pinacidil-activated K(ATP) channel activity in adult rat and neonatal mouse ventricular myocytes. The alpha1-adrenoceptor agonist methoxamine (MTX) reversibly inhibited the pinacidil-activated K(ATP) current in a concentration-dependent manner (IC50 20.9+/-6.6 micromol/L). This inhibition did not occur when the specific alpha1-adrenoceptor antagonist, prazosin, was present. An involvement of G proteins is suggested by the ability of GDPbetaS to prevent this response. Blockade of PLC by U-73122 (2 micromol/L) or neomycin (2 mmol/L) attenuated the MTX-induced inhibition of K(ATP) channel activity. In contrast, the MTX response was unaffected by protein kinase C inhibition or stimulation by H-7 (100 micro mol/L) or phorbol 12,13-didecanoate. The MTX-induced inhibition became irreversible in the presence of wortmannin (20 micro mol/L), an inhibitor of phosphatidylinositol-4 kinase, which is expected to prevent membrane PIP2 replenishment. In excised inside-out patch membranes, pinacidil induced a significantly rightward shift of ATP sensitivity of the channel. This phenomenon was reversed by pretreatment of myocytes with MTX. Direct visualization of PIP2 subcellular distribution using a PLCdelta pleckstrin homology domain-green fluorescent protein fusion constructs revealed reversible translocation of green fluorescent protein fluorescence from the membrane to the cytosol after alpha1-adrenoceptor stimulation. Our data demonstrate that alpha1-adrenoceptor stimulation reduces the membrane PIP2 level, which in turn inhibits pinacidil-activated K(ATP) channels.
Subject(s)
Adenosine Triphosphate/pharmacology , Phosphatidylinositol 4,5-Diphosphate/metabolism , Pinacidil/antagonists & inhibitors , Potassium Channels/physiology , Receptors, Adrenergic, alpha-1/physiology , Ventricular Function , Adrenergic alpha-1 Receptor Agonists , Adrenergic alpha-Agonists/pharmacology , Androstadienes/pharmacology , Animals , Animals, Newborn , Cell Membrane/metabolism , Cells, Cultured , Electric Conductivity , Enzyme Inhibitors/pharmacology , GTP-Binding Proteins/physiology , Heart Ventricles/drug effects , Heart Ventricles/metabolism , Methoxamine/pharmacology , Mice , Phosphoinositide-3 Kinase Inhibitors , Protein Kinase C/physiology , Rats , Rats, Sprague-Dawley , Type C Phospholipases/physiology , WortmanninABSTRACT
Ethanol and dimethylsulfoxide are commonly used as diluents for water-insoluble drugs. Both are antioxidants. An earlier study of cats presented pharmacological evidence indicating that oxidants could open the K(ATP) ion channel in cerebral surface arterioles [pial arterioles] and that antioxidants including dimethylsulfoxide and L-cysteine prevented opening of these channels. Ethanol was not tested. The present study extends the older observations to a second species, the rat, and examines ethanol as well as dimethylsulfoxide and L-cysteine. A microscope and image splitter were used to measure arteriolar diameters under a closed cranial window in pentobarbital-anesthetized, paralyzed rats. Drugs were topically applied. Dose-dependent dilations produced by two well-established openers of the K(ATP) ion channel were inhibited in dose-dependent manner by ethanol at doses from 0.01% to 0.075%. Above this dose, the effect disappeared. Dilation by sodium nitroprusside was not affected. Dimethylsulfoxide and L-cysteine inhibited dilation produced by pinacidil. Dimethylsulfoxide inhibited pinacidil in a dose-dependent manner at doses from 0.01% to 0.2%. L-Cysteine inhibited pinacidil. Since all the inhibitory drugs have antioxidant properties, their effect may be a reflection of that property as suggested in an earlier paper. Ethanol and dimethylsulfoxide inhibited in doses frequently present when these agents are used as solvents. When investigators use these solvents to dissolve water-insoluble, topically applied drugs, we suggest that they first test the possibility that their observations are being made under conditions in which opening of the K(ATP) ion channel is inhibited.
Subject(s)
Cerebral Arteries/drug effects , Dimethyl Sulfoxide/pharmacology , Ethanol/pharmacology , Potassium Channels/drug effects , Animals , Cromakalim/antagonists & inhibitors , Cysteine/pharmacology , Male , Nitroprusside/pharmacology , Pinacidil/antagonists & inhibitors , Rats , Rats, Sprague-Dawley , Solutions , Time Factors , Vasodilation/drug effects , Vasodilator Agents/antagonists & inhibitors , Vasodilator Agents/pharmacologyABSTRACT
1. Mitochondrial dysfunction, secondary to excessive accumulation of Ca2+, has been implicated in cardiac injury. We here examined the action of potassium channel openers on mitochondrial Ca2+ homeostasis, as these cardioprotective ion channel modulators have recently been shown to target a mitochondrial ATP-sensitive K+ channel. 2. In isolated cardiac mitochondria, diazoxide and pinacidil decreased the rate and magnitude of Ca2+ uptake into the mitochondrial matrix with an IC50 of 65 and 128 microM, respectively. At all stages of Ca2+ uptake, the potassium channel openers depolarized the mitochondrial membrane thereby reducing Ca2+ influx through the potential-dependent mitochondrial uniporter. 3. Diazoxide and pinacidil, in a concentration-dependent manner, also activated release of Ca2+ from mitochondria. This was prevented by cyclosporin A, an inhibitor of Ca2+ release through the mitochondrial permeability transition pore. 4. Replacement of extramitochondrial K+ with mannitol abolished the effects of diazoxide and pinacidil on mitochondrial Ca2+, while the K+ ionophore valinomycin mimicked the effects of the potassium channel openers. 5. ATP and ADP, which block K+ flux through mitochondrial ATP-sensitive K+ channels, inhibited the effects of potassium channel openers, without preventing the action of valinomycin. 6. In intact cardiomyocytes, diazoxide also induced mitochondrial depolarization and decreased mitochondrial Ca2+ content. These effects were inhibited by the mitochondrial ATP-sensitive K+ channel blocker 5-hydroxydecanoic acid. 7. Thus, potassium channel openers prevent mitochondrial Ca2+ overload by reducing the driving force for Ca2+ uptake and by activating cyclosporin-sensitive Ca2+ release. In this regard, modulators of an ATP-sensitive mitochondrial K+ conductance may contribute to the maintenance of mitochondrial Ca2+ homeostasis.
Subject(s)
Calcium/metabolism , Membrane Proteins/agonists , Mitochondria, Heart/metabolism , Adenosine Diphosphate/pharmacology , Adenosine Triphosphate/pharmacology , Animals , Cyclosporine/antagonists & inhibitors , Cyclosporine/pharmacology , Diazoxide/antagonists & inhibitors , Diazoxide/pharmacology , In Vitro Techniques , Ionophores/pharmacology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Membrane Proteins/metabolism , Microscopy, Confocal , Mitochondria, Heart/drug effects , Myocardium/cytology , Myocardium/metabolism , Oxygen Consumption/drug effects , Permeability , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Potassium Channels , Rats , Rats, Sprague-Dawley , Valinomycin/pharmacologyABSTRACT
Using analogs of known vascular ATP-sensitive K+ channel (KATP) blockers, we identified compounds with a wide range of potencies (over 500-fold) in their capacity to block the hypotensive response of 0.2 mg/kg pinacidil in rats. The most potent of these, U-97025E, belongs to a newly disclosed class of compounds, the cyanoguanidines. U-97025E at 0.04 mg/kg blocked 50% of the depressor response induced by 0.2 mg/kg pinacidil. The maximal natriuresis induced by U-97025E (0.4 mg/kg i.v.) increased Na+ excretion by approximately 60%. This natriuresis is of the same magnitude as that induced by thiazide without any effect on K+ excretion. We found a high degree of correlation between natriuretic potency and the capacity to block the blood pressure lowering effects of pinacidil, both among closely related analogs and dissimilar compounds. These findings imply an obligatory rather than incidental relationship between vascular KATP blockade and natriuresis. The exact molecular link of the vascular and renal effects remains to be determined.
Subject(s)
Natriuresis/drug effects , Pinacidil/pharmacology , Potassium Channel Blockers , Potassium Channels , ATP-Binding Cassette Transporters , Analysis of Variance , Animals , Antihypertensive Agents/antagonists & inhibitors , Antihypertensive Agents/pharmacology , Blood Pressure/drug effects , Dose-Response Relationship, Drug , Glomerular Filtration Rate/drug effects , Guanidines/pharmacology , Heart Rate/drug effects , KATP Channels , Kidney/drug effects , Kidney/physiology , Male , Nitriles/pharmacology , Pinacidil/antagonists & inhibitors , Potassium Channels, Inwardly Rectifying , Rats , Rats, Sprague-DawleyABSTRACT
In hypertension, impairment of hyperpolarization by K+ efflux through ATP-sensitive K+ (K(ATP)) channels may contribute to the elevated renal vascular resistance. To elucidate such a role for K(ATP) channels in the renal vasculature, we used micropuncture techniques to examine the effect of K(ATP) channel opener, pinacidil (0.15 mg/h per kg body wt i.v.), on renal and glomerular haemodynamics in spontaneously hypertensive rats (SHR) and in normotensive controls (Wistar Kyoto, WKY). Since pinacidil reduced blood pressure significantly in both groups, the abdominal aorta was clamped before pinacidil administration to yield a renal perfusion pressure equivalent to that during pinacidil infusion. Pinacidil significantly decreased renal vascular resistance in both groups, but the relative change from baseline value was greater in WKY than in SHR. These effects of pinacidil were abolished by pretreatment with glibenclamide (3 mg/kg body wt i.v.). Proximal tubular stop-flow pressure (Psf), an index of glomerular capillary pressure, was significantly elevated by pinacidil infusion in WKY, a response abolished by pretreatment with glibenclamide, but not in SHR. The tubuloglomerular feedback response of Psf was not affected by pinacidil in either group. These data suggest that the activity of K(ATP) channels in SHR may be attenuated in the renal microvasculature. This may contribute to the elevated vascular tone in the renal preglomerular vasculature in SHR.
Subject(s)
Antihypertensive Agents/pharmacology , Hemodynamics/drug effects , Kidney/drug effects , Pinacidil/pharmacology , Potassium Channels/drug effects , Animals , Antihypertensive Agents/antagonists & inhibitors , Glyburide/pharmacology , Hypoglycemic Agents/pharmacology , Kidney/blood supply , Pinacidil/antagonists & inhibitors , Punctures , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Species SpecificityABSTRACT
ATP-sensitive potassium channel openers are used as vasodilators in the treatment of cardiovascular disorders. The effects of i.v. anaesthetics on arterial relaxation induced by ATP-sensitive potassium channel openers have not been studied. Therefore, in this study, we have examined if thiopental (thiopentone) and propofol affect the vascular response to the ATP-sensitive potassium channel openers, cromakalim and pinacidil, in the isolated rat aorta. Rings of rat thoracic aortas without endothelium were suspended for isometric force recording. Concentration-response curves were obtained in a cumulative manner. During submaximal contractions with phenylephrine 0.3 mumol litre-1, relaxation after cromakalim 0.1-30 mumol litre-1, pinacidil 0.1-30 mumol litre-1 and papaverine 0.1-300 mumol litre-1 was demonstrated. Thiopental 30-300 mumol litre-1, propofol 10-100 mumol litre-1, 10% Intralipid 45 microliters or glibenclamide 5 mumol litre-1 were applied 15 min before addition of phenylephrine. During contractions with phenylephrine, cromakalim and pinacidil induced concentration-dependent relaxation. A selective ATP-sensitive potassium channel antagonist, glibenclamide 5 mumol litre-1, abolished this relaxation, whereas it did not affect relaxation produced by papaverine. Thiopental concentrations > 30 mumol litre-1 significantly impaired relaxation produced by cromakalim or pinacidil. Propofol concentrations > 10 mumol litre-1 also significantly reduced relaxation produced by cromakalim or pinacidil, whereas Intralipid was ineffective. Thiopental 300 mumol litre-1 and propofol 100 mumol litre-1 did not alter relaxation produced by papaverine. These results suggest that the i.v. anaesthetics, thiopental and propofol, impaired vasodilatation mediated by ATP-sensitive potassium channels in vascular smooth muscle cells.
Subject(s)
Anesthetics, Intravenous/pharmacology , Potassium Channels/drug effects , Propofol/pharmacology , Thiopental/pharmacology , Vasodilation/drug effects , Adenosine Triphosphate/physiology , Animals , Aorta, Thoracic/drug effects , Aorta, Thoracic/physiology , Cromakalim/antagonists & inhibitors , Cromakalim/pharmacology , Dose-Response Relationship, Drug , Male , Pinacidil/antagonists & inhibitors , Pinacidil/pharmacology , Rats , Rats, Inbred WKY , Vasodilator Agents/antagonists & inhibitors , Vasodilator Agents/pharmacologyABSTRACT
AIM: To study the effects of potassium channel openers (PCO) on cytosolic free calcium ([Ca2+]i) changes and their possible mechanisms in vascular smooth muscle cells (VSMC). METHODS: Cultured rabbit aortic VSMC were treated with Fura-2 AM 2.5 mumol.L-1 at 37 degrees C for 50 min. The PCO were pinacidil (Pin), nicorandil (Nic), lemakalim (Lem), and RP 49356 (RP). [Ca2+]i level was measured by fluorospectrometer. RESULTS: [Ca2+]i increase induced by K+ 30 mmol.L-1 was weakly inhibited by Pin, Nic, Lem, and RP (441 +/- 23, 455 +/- 48, 451 +/- 22, 370 +/- 31 vs 544 +/- 40 nmol.L-1, P < 0.01). ATP (0.1 mmol.L-1)-induced peak and sustained [Ca2+]i increase were inhibited by these agents in a concentration-dependent manner. The effects of Pin, Lem, and RP were completely canceled (peak phase: 549 +/- 39, 540 +/- 30, 564 +/- 13 vs 541 +/- 39 nmol.L-1; sustained phase: 413 +/- 25, 364 +/- 16, 377 +/- 11 vs 380 +/- 8 nmol.L-1), but that of Nic was only partially blocked (peak phase: 453 +/- 31 vs 541 nmol.L-1; sustained phase: 348 +/- 19 vs 380 +/- 8 nmol.L-1, P < 0.01) by glibenclamide (Gli, 10 mumol.L-1). Pretreated with the Pin, Nic, Lem, and RP (10 mumol.L-1), the peak [Ca2+]i elevation induced by ATP was reduced in the Ca(2+)-free solution (129 +/- 17, 142 +/- 21, 136 +/- 14, 114 +/- 9 vs 258 +/- 32 nmol.L-1, P < 0.01). CONCLUSION: Pin, Nic, Lem, and RP inhibited ATP-induced [Ca2+]i increase, associated with decreases of both Ca2+ release from intracellular store and Ca2+ influx from extracellular store.