ABSTRACT
Expression, purification, and functional reconstitution of mammalian ion channels are often challenging. Heterologous expression of mammalian channels in bacteria can be advantageous due to unrelated protein environment and the lack of risk of copurification of endogenous proteins, e.g., accessory channel subunits that can influence the channel activity. Also, direct recording of channel activity could be challenging due to their intracellular localization like in the case of mitochondrial channels. The activity of purified channels can be characterized at the single-molecule level by electrophysiological techniques, such as planar lipid bilayers (PLB). In this work, we describe a simple approach to accomplish PLB recording of the activity of single renal outer medullary potassium channels ROMK expressed in E. coli. We focused on the ROMK2 isoform that is present at low levels in the mitochondria and can be responsible for mitoKATP activity. We screened for the best construct to express the codon-optimized ROMK proteins with a 6xHis tag for protein purification. The strategy involved the use of optimal styrene-maleic acid (SMA) copolymer, which forms so-called polymer nanodiscs, to solubilize and purify ROMK-containing SMA lipid particles (SMALPs), which were amenable for fusion with PLB. Reconstituted ROMK channels exhibited ion selectivity, rectification, and pharmacological properties, which are in agreement with previous work on ROMK channels.
Subject(s)
Maleates/chemistry , Nanostructures/chemistry , Potassium Channels, Inwardly Rectifying/chemistry , Styrene/chemistry , Humans , Potassium Channels, Inwardly Rectifying/biosynthesis , Potassium Channels, Inwardly Rectifying/genetics , Potassium Channels, Inwardly Rectifying/isolation & purification , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/isolation & purificationABSTRACT
The inwardly rectifying K+ (Kir) channel, Kir6.2, plays critical roles in physiological processes in the brain, heart, and pancreas. Although Kir6.2 has been extensively studied in numerous expression systems, a comprehensive description of an expression and purification protocol has not been reported. We expressed and characterized a recombinant Kir6.2, with an N-terminal decahistidine tag, enhanced green fluorescent protein (eGFP) and deletion of C-terminal 26 amino acids, in succession, denoted eGFP-Kir6.2Δ26. eGFP-Kir6.2Δ26 was expressed in HEK293â¯cells and a purification protocol developed. Electrophysiological characterization showed that eGFP-Kir6.2Δ26 retains native single channel conductance (64⯱â¯3.3â¯pS), mean open times (τ1â¯=â¯0.72â¯ms, τ2â¯=â¯15.3â¯ms) and ATP affinity (IC50â¯=â¯115⯱â¯25⯵M) when expressed in HEK293â¯cells. Detergent screening using size exclusion chromatography (SEC) identified Fos-choline-14 (FC-14) as the most suitable surfactant for protein solubilization, as evidenced by maintenance of the native tetrameric structure in SDS-PAGE and western blot analysis. A two-step scheme using Co2+-metal affinity chromatography and SEC was implemented for purification. Purified protein activity was assessed by reconstituting eGFP-Kir6.2Δ26 in black lipid membranes (BLMs) composed of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG), l-α-phosphatidylinositol-4,5-bisphosphate (PIP2) in a 89.5:10:0.5â¯mol ratio. Reconstituted eGFP-Kir6.2Δ26 displayed similar single channel conductance (61.8⯱â¯0.54â¯pS) compared to eGFP-Kir6.2Δ26 expressed in HEK293 membranes; however, channel mean open times increased (τ1â¯=â¯7.9â¯ms, τ2â¯=â¯61.9â¯ms) and ATP inhibition was significantly reduced for eGFP-Kir6.2Δ26 reconstituted into BLMs (IC50â¯=â¯3.14⯱â¯0.4â¯mM). Overall, this protocol should be foundational for the production of purified Kir6.2 for future structural and biochemical studies.
Subject(s)
Potassium Channels, Inwardly Rectifying/genetics , Potassium Channels, Inwardly Rectifying/metabolism , Adenosine Triphosphate/metabolism , Amino Acid Sequence , Chromatography, Affinity , Chromatography, Gel , Gene Expression , Green Fluorescent Proteins/analysis , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/isolation & purification , Green Fluorescent Proteins/metabolism , HEK293 Cells , Humans , Lipid Bilayers/metabolism , Patch-Clamp Techniques , Potassium Channels, Inwardly Rectifying/analysis , Potassium Channels, Inwardly Rectifying/isolation & purification , Recombinant Fusion Proteins/analysis , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/isolation & purification , Recombinant Fusion Proteins/metabolism , Sequence Deletion , Solubility , Transfection/methodsABSTRACT
BACKGROUND: No substantial work has been conducted to date in context to cationic proteins with antilithiatic activity. We explored the antilithiatic cationic proteins present in human calcium oxalate (CaOx) stones and also examined their molecular interactions with calcium oxalate crystals in silico. METHODS: Proteins were isolated from the matrix of human CaOx containing kidney stones. Proteins having MW>3 kDa were subjected to cation exchange chromatography followed by molecular-sieve chromatography. The effect of these purified cationic proteins was tested against CaOx nucleation and growth and on oxalate injured MDCK cells for their activity. Proteins were identified by MALDI-TOF MS. Molecular interaction studies with COM crystals in silico were also investigated. RESULTS: Three antilithiatic cationic proteins were identified as histone-lysine N-methyltransferase, inward rectifier K channel and protein Wnt-2 (MW~53, ~44, and ~42 kDa respectively) by MALDI-TOF MS based on database search with MASCOT server. Further molecular modeling calculations revealed the mode of interaction of these proteins with CaOx at the molecular level. CONCLUSION: We identified histone-lysine N-methyltransferase, inward rectifier K channel and protein Wnt-2 as novel antilithiatic proteins which play a vital role in the kidney function and have been associated with various kidney diseases.
Subject(s)
Calcium Oxalate/chemistry , Histone-Lysine N-Methyltransferase/chemistry , Kidney Calculi/chemistry , Potassium Channels, Inwardly Rectifying/chemistry , Wnt2 Protein/chemistry , Adult , Animals , Binding Sites , Cations , Cell Survival/drug effects , Chromatography, Gel , Chromatography, Ion Exchange , Crystallization , Cytoprotection , Dogs , Histone-Lysine N-Methyltransferase/isolation & purification , Histone-Lysine N-Methyltransferase/pharmacology , Humans , Madin Darby Canine Kidney Cells , Molecular Docking Simulation , Potassium Channels, Inwardly Rectifying/isolation & purification , Potassium Channels, Inwardly Rectifying/pharmacology , Protein Binding , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Wnt2 Protein/isolation & purification , Wnt2 Protein/pharmacologyABSTRACT
The inward rectifier family of potassium (KCNJ) channels regulate vital cellular processes including cell volume, electrical excitability, and insulin secretion. Dysfunction of different isoforms have been linked to numerous diseases including Bartter's, Andersen-Tawil, Smith-Magenis Syndromes, Type II diabetes mellitus, and epilepsy, making them important targets for therapeutic intervention. Using a family-based approach, we succeeded in expressing 10 of 11 human KCNJ channels tested in Saccharomyces cerevisiae. GFP-fusion proteins showed that these channels traffic correctly to the plasma-membrane suggesting that the protein is functional. A 2-step purification process can be used to purify the KCNJ channels to >95% purity in a mono-dispersed form. After incorporation into liposomes, (86)Rb(+) flux assays confirm the functionality of the purified proteins as inward rectifier potassium channels.
Subject(s)
Biochemistry/methods , Potassium Channels, Inwardly Rectifying/isolation & purification , Potassium Channels, Inwardly Rectifying/metabolism , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/metabolism , Cloning, Molecular , Humans , Protein Transport , Subcellular Fractions/metabolismABSTRACT
The inward-rectifying K(+) channel KAT1 is expressed mainly in Arabidopsis thaliana guard cells. The purification of functional KAT1 has never been reported. We investigated the extraction of the plant K(+) channel KAT1 with different detergents, as an example for how to select detergents for purifying a eukaryotic membrane protein. A KAT1-GFP fusion protein was used to screen a library of 46 detergents for the effective solubilization of intact KAT1. Then, a "test set" of three detergents was picked for further analysis, based on their biochemical characteristics and availability. The combination use of the selected detergents enabled the effective purification of functional KAT1 with affinity and gel-filtration chromatography.
Subject(s)
Arabidopsis Proteins/isolation & purification , Detergents/chemistry , Potassium Channels, Inwardly Rectifying/isolation & purification , Recombinant Fusion Proteins/isolation & purification , Animals , Arabidopsis Proteins/chemistry , Arabidopsis Proteins/genetics , Chromatography, Affinity/methods , Chromatography, Gel , Fluorescence , Green Fluorescent Proteins/chemistry , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/isolation & purification , Histidine/chemistry , Histidine/genetics , Histidine/isolation & purification , Insecta/cytology , Potassium Channels, Inwardly Rectifying/chemistry , Potassium Channels, Inwardly Rectifying/genetics , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/geneticsABSTRACT
ATP-sensitive potassium (K(ATP)) channels conduct potassium ions across cell membranes and thereby couple cellular energy metabolism to membrane electrical activity. Here, we report the heterologous expression and purification of a functionally active K(ATP) channel complex composed of pore-forming Kir6.2 and regulatory SUR1 subunits, and determination of its structure at 18 A resolution by single-particle electron microscopy. The purified channel shows ATP-ase activity similar to that of ATP-binding cassette proteins related to SUR1, and supports Rb(+) fluxes when reconstituted into liposomes. It has a compact structure, with four SUR1 subunits embracing a central Kir6.2 tetramer in both transmembrane and cytosolic domains. A cleft between adjacent SUR1s provides a route by which ATP may access its binding site on Kir6.2. The nucleotide-binding domains of adjacent SUR1 appear to interact, and form a large docking platform for cytosolic proteins. The structure, in combination with molecular modelling, suggests how SUR1 interacts with Kir6.2.
Subject(s)
ATP-Binding Cassette Transporters/chemistry , ATP-Binding Cassette Transporters/physiology , Potassium Channels, Inwardly Rectifying/chemistry , Potassium Channels, Inwardly Rectifying/physiology , Potassium Channels/chemistry , Potassium Channels/physiology , Receptors, Drug/chemistry , Receptors, Drug/physiology , ATP-Binding Cassette Transporters/ultrastructure , Amino Acid Sequence , Animals , Cryoelectron Microscopy , Mice , Molecular Sequence Data , Potassium Channels/ultrastructure , Potassium Channels, Inwardly Rectifying/isolation & purification , Potassium Channels, Inwardly Rectifying/ultrastructure , Protein Structure, Tertiary , Rats , Receptors, Drug/ultrastructure , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/physiology , Recombinant Fusion Proteins/ultrastructure , Sulfonylurea ReceptorsABSTRACT
Arterial ATP-sensitive K+ (K(ATP)) channels are critical regulators of vascular tone, forming a focal point for signaling by many vasoactive transmitters that alter smooth muscle contractility and so blood flow. Clinically, these channels form the target of antianginal and antihypertensive drugs, and their genetic disruption leads to hypertension and sudden cardiac death through coronary vasospasm. However, whereas the biochemical basis of K(ATP) channel modulation is well-studied, little is known about the structural or spatial organization of the signaling pathways that converge on these channels. In this study, we use discontinuous sucrose density gradients and Western blot analysis to show that K(ATP) channels localize with an upstream signaling partner, adenylyl cyclase, to smooth muscle membrane fractions containing caveolin, a protein found exclusively in cholesterol and sphingolipid-enriched membrane invaginations known as caveolae. Furthermore, we show that an antibody against the K(ATP) pore-forming subunit, Kir6.1 co-immunoprecipitates caveolin from arterial homogenates, suggesting that Kir6.1 and caveolin exist together in a complex. To assess whether the colocalization of K(ATP) channels and adenylyl cyclase to smooth muscle caveolae has functional significance, we disrupt caveolae with the cholesterol-depleting agent, methyl-beta-cyclodextrin. This reduces the cAMP-dependent protein kinase A-sensitive component of whole-cell K(ATP) current, indicating that the integrity of caveolae is important for adenylyl cyclase-mediated channel modulation. These results suggest that to be susceptible to protein kinase A-dependent activation, arterial K(ATP) channels need to be localized in the same lipid compartment as adenylyl cyclase; the results also provide the first indication of the spatial organization of signaling pathways that regulate K(ATP) channel activity.
Subject(s)
ATP-Binding Cassette Transporters/physiology , Adenylyl Cyclases/physiology , Aorta/ultrastructure , Caveolae/physiology , Caveolins/physiology , Cyclic AMP-Dependent Protein Kinases/physiology , Guanosine Diphosphate/analogs & derivatives , Myocytes, Smooth Muscle/physiology , Potassium Channels, Inwardly Rectifying/physiology , Theophylline/analogs & derivatives , Adenosine Triphosphate/pharmacology , Animals , Aorta/enzymology , Aorta/physiology , Calcitonin Gene-Related Peptide/pharmacology , Caveolae/chemistry , Caveolae/drug effects , Caveolae/enzymology , Caveolin 1 , Caveolins/analysis , Cell Compartmentation , Cell Fractionation , Cholesterol/analysis , Glyburide/pharmacology , Guanosine Diphosphate/pharmacology , Ion Transport/drug effects , Isoenzymes/physiology , KATP Channels , Male , Membrane Lipids/analysis , Mesenteric Arteries/chemistry , Mesenteric Arteries/enzymology , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/physiology , Myocytes, Smooth Muscle/chemistry , Myocytes, Smooth Muscle/enzymology , Patch-Clamp Techniques , Peptide Fragments/pharmacology , Pinacidil/pharmacology , Potassium/metabolism , Potassium Channels, Inwardly Rectifying/drug effects , Potassium Channels, Inwardly Rectifying/isolation & purification , Propranolol/pharmacology , Rats , Rats, Wistar , Sphingolipids/analysis , Theophylline/pharmacology , Thionucleotides/pharmacologyABSTRACT
Ion channels were studied using the whole-cell patch clamp technique in bovine retinal and choroidal microvascular endothelial cells (MVEC) cultured under the same conditions. The two types of MVEC expressed inward currents at hyperpolarizing voltage steps and showed small outward currents at depolarizing steps. The extrapolated reversal potentials of the inward currents were near to the potassium equilibrium potential. Cs(+) and the K(+) channel blocker TEA reduced the amplitudes of the currents indicating the selectivity and permeability for potassium. This was confirmed by changes of outside K(+) concentration shifting the I-V curves to the right. RT-PCR studies revealed the presence of mRNA of Kir2.1, an inwardly rectifying K(+) channel, in retinal and choroidal MVEC. The profile of the small outward currents is related to the Kv family but not identical with the Kv1.4 subtype.
Subject(s)
Choroid/cytology , Endothelium, Vascular/metabolism , Potassium Channels, Inwardly Rectifying/isolation & purification , Retina/cytology , Animals , Cattle , Cells, Cultured , Cesium/pharmacology , DNA, Complementary/analysis , Endothelium, Vascular/physiology , Fluorescent Antibody Technique , Lipoproteins, LDL , Membrane Potentials/drug effects , Membrane Potentials/physiology , Patch-Clamp Techniques/methods , Potassium/metabolism , Potassium/pharmacology , Potassium Channels, Inwardly Rectifying/classification , Potassium Channels, Inwardly Rectifying/genetics , Potassium Channels, Inwardly Rectifying/physiology , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction/methods , Tetraethylammonium Compounds/pharmacologyABSTRACT
Inhibition of ATP-sensitive K(+) (K(ATP)) channels by ATP, a process presumably initiated by binding of ATP to the pore-forming subunit, Kir6.2, is reduced in the presence of phosphoinositides (PPIs). Previous studies led to the hypothesis that PPIs compromise ATP binding. Here, this hypothesis was tested using purified Kir6.2. We show that PPIs bind purified Kir6.2 in an isomer-specific manner, that biotinylated ATP analogs photoaffinity label purified Kir6.2, and that this labeling is weakened in the presence of PPIs. Patch-clamp measurements confirmed that these ATP analogs inhibited Kir6.2 channels, and that PPIs decreased the level of inhibition. These results indicate that interaction of PPIs with Kir6.2 impedes ATP-binding activity. The PPI regulation of ATP binding revealed in this study provides a putative molecular mechanism that is potentially pivotal to the nucleotide sensitivity of K(ATP) channels.