ABSTRACT
Microwave irradiation has become a popular heating technique in organic synthesis, mainly due to its short reaction times, solventless reactions, and, sometimes, higher yields. Additionally, microwave irradiation lowers energy consumption and, consequently, is ideal for optimization processes. Moreover, there is evidence that microwave irradiation can improve the regioselectivity and stereoselectivity aspects of vital importance in synthesizing bioactive compounds. These crucial features of microwave irradiation contribute to its inclusion in green chemistry procedures. Since 2003, the use of microwave-assisted organic synthesis has become common in our laboratory, making our group one of the first Portuguese research groups to implement this heating source in organic synthesis. Our achievements in the transformation of heterocyclic compounds, such as (E/Z)-3-styryl-4H-chromen-4-ones, (E)-3-(2-hydroxyphenyl)-4-styryl-1H-pyrazole, (E)-2-(4-arylbut-1-en-3-yn-1-yl)-4H-chromen-4-ones, or (E)-2-[2-(5-aryl-2-methyl-2H-1,2,3-triazol-4-yl)vinyl]-4H-chromen-4-ones, will be discussed in this review, highlighting the benefits of microwave irradiation use in organic synthesis.
Subject(s)
Chromones/chemical synthesis , Heating/methods , Microwaves , Pyrazoles/chemical synthesis , Quinolones/chemical synthesis , Chemistry, Pharmaceutical/methods , Chromones/radiation effects , Combinatorial Chemistry Techniques/methods , Enzyme Inhibitors/chemical synthesis , Humans , Molecular Structure , Pyrazoles/radiation effects , Quinolones/radiation effectsABSTRACT
The labelling and imaging of mitochondria and nucleolus have attracted great attention because of the involvement of these cellular organelles in critical cellular activities. Therefore, a large number of mitochondria- or nucleolus-labelling probes have been developed throughout the world. However, in the current study, we successfully developed two pyrazole-based, copper-linked triphenylphosphine-coupled emissive metallo-complexes (C1 and C2) for the simultaneous visualization of mitochondria and nucleolus in a single run. These complexes were very inexpensive and could be synthesized by a simple one-pot multicomponent reaction scheme. The complexes were very specific, and only a small concentration of 5 µM was found to be sufficient to probe both the organelles efficiently. Additionally, even under a shorter incubation period (half hour), the fluorescence intensity from the cells was appreciable. Also, both the compounds were found to be photostable when torched with 10% of a 100 mW laser for up to 10 min. All these results indicate that both the complexes may contribute towards the future development of cell imaging tools. To the best of our knowledge, this is the first report on the development of multifunctional live cell imaging tools for simultaneous mitochondria and nucleolus imaging and within the shortest incubation time of about 30 minutes.
Subject(s)
Cell Nucleolus/metabolism , Coordination Complexes/chemistry , Fluorescent Dyes/chemistry , Mitochondria/metabolism , Organophosphorus Compounds/chemistry , Pyrazoles/chemistry , Coordination Complexes/chemical synthesis , Coordination Complexes/radiation effects , Copper/chemistry , Copper/radiation effects , Density Functional Theory , Fluorescent Dyes/chemical synthesis , Fluorescent Dyes/radiation effects , HeLa Cells , Humans , Lasers , Microscopy, Confocal/methods , Microscopy, Fluorescence/methods , Models, Chemical , Organophosphorus Compounds/chemical synthesis , Organophosphorus Compounds/radiation effects , Pyrazoles/chemical synthesis , Pyrazoles/radiation effects , Ultraviolet RaysABSTRACT
The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.
Subject(s)
DNA, Catalytic/chemistry , DNA, Catalytic/radiation effects , Azo Compounds/chemistry , Azo Compounds/radiation effects , Benzothiazoles/chemistry , Catalysis/radiation effects , DNA, Catalytic/genetics , G-Quadruplexes/radiation effects , Infrared Rays , Isomerism , Nucleic Acid Hybridization/radiation effects , Oxidation-Reduction , Pyrazoles/chemistry , Pyrazoles/radiation effects , Sulfonic Acids/chemistryABSTRACT
A photoswitchable arylazopyrazole (AAP) derivative binds with cucurbit[8]uril (CB[8]) and methylviologen (MV2+ ) to form a 1:1:1 heteroternary host-guest complex with a binding constant of Ka =2×103 m-1 . The excellent photoswitching properties of AAP are preserved in the inclusion complex. Irradiation with light of a wavelength of 365 and 520â nm leads to quantitative E- to Z- isomerization and vice versa, respectively. Formation of the Z-isomer leads to dissociation of the complex as evidenced using 1 Hâ NMR spectroscopy. AAP derivatives are then used to immobilize bioactive molecules and photorelease them on demand. When Arg-Gly-Asp-AAP (AAP-RGD) peptides are attached to surface bound CB[8]/MV2+ complexes, cells adhere and can be released upon irradiation. The heteroternary host-guest system offers highly reversible binding properties due to efficient photoswitching and these properties are attractive for designing smart surfaces.
Subject(s)
Macrocyclic Compounds/chemistry , Oligopeptides/chemistry , Paraquat/chemistry , Pyrazoles/chemistry , Animals , Cell Adhesion , Cell Line , Humans , Isomerism , Kinetics , Light , Macrocyclic Compounds/radiation effects , Mice , Oligopeptides/radiation effects , Paraquat/radiation effects , Photochemical Processes , Pyrazoles/radiation effects , Surface PropertiesABSTRACT
Agrochemicals such as the insecticide Fipronil that bear fluoro groups are generally fat-soluble and nearly insoluble in water, so that their photodegradation in a heterogeneous aqueous gallium oxide dispersion presents some challenges. This article examined the photodegradation of this insecticide by solubilizing it through the addition of organic solvents (EtOH, MeOH, THF, 1,4-dioxane and ethylene glycol) to an aqueous medium and then subjecting the insecticide to 254 nm UVC radiation under photocatalytically inert (Ga2O3/N2) and air-equilibrated (Ga2O3/O2) conditions, as well as photochemically in the absence of Ga2O3 but also under inert and air-equilibrated conditions. Defluorination, dechlorination, desulfonation and denitridation of Fipronil were examined in mixed aqueous/organic media (10, 25 and 50 vol% in organic solvent). After 3 h of UVC irradiation (50 vol% mixed media) defluorination with Ga2O3/N2 was â¼65% greater than in aqueous media, and ca. 80% greater than the direct photolysis of Fipronil under inert (N2) conditions; under air-equilibrated conditions both Ga2O3-photocatalyzed and photochemical defluorination were significantly lower than in aqueous media. Dechlorination of Fipronil was â¼160% (Ga2O3/N2) and 140% (photochemically, N2) greater than in aqueous media; under air-equilibrated conditions, both photocatalyzed and photochemical formation of Cl(-) ions in mixed media fell rather short relative to aqueous media. The photocatalyzed (Ga2O3/N2) and photochemical (N2) conversion of the sulfur group in Fipronil to SO4(2(-)) ions was ca. 20% and 30% greater, respectively, in mixed media, while under air-equilibrated conditions photocatalyzed desulfonation was nearly twofold less than in the aqueous phase; direct photolysis showed little variations in mixed media. Denitridation of the nitrogens in Fipronil occurred mostly through the formation of ammonia (as NH4(+)) under all conditions with negligible quantities of NO3(-); again mixed media offered enhanced denitridation, particularly under inert N2 conditions. Time-of-flight electrospray (TOF-MS/ESI(-)) mass spectrometry revealed a fairly large number of intermediates formed in the degradation of Fipronil, particularly under photocatalytic conditions. Only a couple of intermediates were identified in the photodegradation and the presence of Ga2O3 enhanced the complexity of an already cumbersome problem owing to the involvement of organic solvents.
Subject(s)
Gallium/chemistry , Insecticides/chemistry , Photochemical Processes , Pyrazoles/chemistry , Ultraviolet Rays , Ammonia/chemistry , Catalysis , Chlorine/chemistry , Ethanol/chemistry , Insecticides/radiation effects , Ions/chemistry , Mass Spectrometry , Molecular Structure , Nitrogen/chemistry , Oxygen/chemistry , Pyrazoles/radiation effects , Solvents/chemistry , Sulfates/chemistry , Water/chemistryABSTRACT
The high frequency dynamics of Indomethacin and Celecoxib glasses has been investigated by inelastic x-ray scattering, accessing a momentum-energy region still unexplored in amorphous pharmaceuticals. We find evidence of phonon-like acoustic dynamics, and determine the THz behavior of sound velocity and acoustic attenuation. Connections with ordinary sound propagation are discussed, along with the relation between fast and slow degrees of freedom as represented by non-ergodicity factor and kinetic fragility, respectively.
Subject(s)
Indomethacin/chemistry , Indomethacin/radiation effects , Pyrazoles/chemistry , Pyrazoles/radiation effects , Sound , Sulfonamides/chemistry , Sulfonamides/radiation effects , Terahertz Radiation , Celecoxib , Molecular Conformation/radiation effects , Radiation DosageABSTRACT
New and high regioselective method of the synthesis of 2,7-diaryl-4,7-dihydropyrazolo[1,5-a]pyrimidine-5-carboxylic acids by reaction of 3-aryl-5-aminopyrazoles with arylidenpyruvic acid at room temperature under ultrasonication was developed and discussed.
Subject(s)
Carboxylic Acids/chemical synthesis , Carboxylic Acids/radiation effects , Pyrazoles/chemistry , Pyrazoles/radiation effects , Pyruvic Acid/chemistry , Pyruvic Acid/radiation effects , Sonication/methods , High-Energy Shock Waves , Radiation Dosage , StereoisomerismABSTRACT
Degradation of Tectilon Yellow 2G (TY2G), an azo dye has been studied by hybrid technique involving pretreatment by sonochemical method and further biological treatment by Pseudomonas putida mutant. Pretreatment experiments were carried out by sonolysis of the dye solution at different concentrations (100-1000 mg/L). Wild type Gram-negative P. putida species isolated from the textile effluent contaminated soil, which was found to be effective towards dye degradation, has been acclimatized so as to consume TY2G as the sole source of nutrition. Mutant strain was obtained from the acclimatized species by random mutagenesis using the chemical mutagen ethidium bromide for various time intervals (6-30 min). The optimum mutagenesis exposure time for obtaining the most efficient species for dye degradation was found to be 18 min. An efficient mutant strain P. putida ACT 1 has been isolated and was used for growth experiments. The mutant strain showed a better growth compared to the wild strain. The substrate utilization kinetics has been modeled using Monod and Haldane model equations of which the Haldane model provided a better fit. The enzyme kinetics of the mutant and wild species was obtained using Michaelis-Menten equation. The mutated species showed better enzyme kinetics towards the degradation of TY2G.
Subject(s)
Pseudomonas putida/physiology , Pyrazoles/isolation & purification , Pyrazoles/metabolism , Sonication/methods , Biodegradation, Environmental , Mutation , Pseudomonas putida/classification , Pyrazoles/radiation effects , Species SpecificityABSTRACT
The synthesis of 5-aryl-1,3-diphenylpyrazole via the reactions of 3-aryl-2,3-epoxy-1-phenyl-1-propanone with phenylhydrazine was carried out in 69-99% yields at room temperature under ultrasound irradiation. This method provides several advantages such as operational simplicity, higher yield and environment friendly.
Subject(s)
Hydrochloric Acid/chemistry , Phenylhydrazines/chemistry , Phenylhydrazines/radiation effects , Propane/chemistry , Propane/radiation effects , Pyrazoles/chemical synthesis , Pyrazoles/radiation effects , Sonication/methods , Catalysis , Hydrochloric Acid/radiation effects , Radiation DosageABSTRACT
Pest Management Professionals commonly use polyethylene or fiberglass tanks for mixing and applying termiticides. We investigated the stability of fipronil (Termidor SC) (0.06%, 0.09% and 0.125% active ingredient) in aqueous solutions stored in polyethylene tanks under sun and shade for 2 week. Chemical analysis of tank-mixed solutions sampled at 0, 24, 48, 72, 168 and 336 h indicated that fipronil remained stable. Our data indicated that polyethylene tanks prevent fipronil from photodegradation and these tanks can be safely used for short term storage (up to 2 week) of liquid termiticides.
Subject(s)
Insecticides/chemistry , Light , Photolysis , Polyethylene/chemistry , Pyrazoles/chemistry , Sunlight , Adsorption , Chromatography, High Pressure Liquid , Insecticides/radiation effects , Linear Models , Pyrazoles/radiation effects , Temperature , Time FactorsABSTRACT
Aqueous solutions of the herbicide azimsulfuron have been treated by a photocatalytic process employing titania nanocrystalline films as photocatalyst. Results showed that solutions of this herbicide at maximum possible concentration can be photodegraded in a time of a few hours by using low intensity UVA radiation comparable with that of the UVA of solar noon. Similar results have also been obtained with simulated solar radiation. Thus heterogeneous photocatalysis can be employed for the treatment of waters polluted by this herbicide.
Subject(s)
Photolysis/drug effects , Pyrazoles/radiation effects , Sulfonamides/radiation effects , Titanium/pharmacology , Water Pollutants , Catalysis , Herbicides/radiation effects , Light , Nanoparticles , Water Pollutants/radiation effectsABSTRACT
Novel pyrazoles and isoxazoles were synthesized from the reaction of the carbanions of 1-aryl-2-(phenylsulphonyl)-ethanone with different hydrazonyl halides and 1-aryl-2-bromo-2-hydroximinoethanone in ethanol, respectively. Reactions were carried out under silent and ultrasonic conditions. In general, it was found that ultrasound irradiations enable some reactions to occur which could not be carried out under silent condition in addition to improvement in reaction times. The products were obtained in high yields and their structures were determined by elemental analyses, spectral data and X-ray diffraction.
Subject(s)
Isoxazoles/chemical synthesis , Isoxazoles/radiation effects , Pyrazoles/chemical synthesis , Pyrazoles/radiation effects , Sulfones/chemistry , Ultrasonics , Crystallography, X-Ray , Indicators and Reagents , Isoxazoles/chemistry , Magnetic Resonance Spectroscopy , Mass Spectrometry , Models, Molecular , Pyrazoles/chemistry , Solvents , Spectrophotometry, Infrared , Sulfones/radiation effectsABSTRACT
The mechanisms of the photochemical isomerization reactions were investigated by using a model system of 1,3,5-trimethylpyrazole ( 1) with the CASSCF (eight-electron/six-orbital active space) and MP2-CAS methods and the 6-311G(d) basis set. Three reaction pathways were examined in the present work. They are denoted as the ring-contraction-ring-expansion path (path I), the internal-cyclization-isomerization path (path II), and the conical-intersection path (path III). Our model investigations suggest that the preferred reaction route for the pyrazoles is as follows: reactant --> Franck-Condon region --> conical intersection --> photoproduct. In particular, the conical-intersection mechanism (path III) found in this work gives a better explanation than the previously proposed two other mechanisms (paths I and II). The theoretical findings also indicate that path III-1 should be favored over path III-2 from a kinetic point of view. This suggests that the quantum yield of 1,2,4-trimethylimidazole ( 2) should be greater than that of 1,2,5-trimethylimidazole ( 3), which supports the available experimental observations. Additionally, we propose a simple p-pi orbital topology model, which can be used as a diagnostic tool to predict the location of the conical intersections, as well as the geometries of the phototransposition products of various heterocycles.
Subject(s)
Computer Simulation , Imidazoles/radiation effects , Molecular Structure , Pyrazoles/radiation effects , Quantum Theory , Ultraviolet Rays , Imidazoles/chemical synthesis , Imidazoles/chemistry , Kinetics , Models, Chemical , Photochemistry , Pyrazoles/chemistryABSTRACT
A safe, functional-group-tolerant and high-throughput version of the trimethylaluminium mediated amide bond formation reaction has been developed in a microreactor system; rimonabant and efaproxiral were prepared to illustrate the utility of the method.
Subject(s)
Alkanes/chemistry , Aluminum/chemistry , Amides/chemical synthesis , Aniline Compounds/chemical synthesis , Piperidines/chemical synthesis , Propionates/chemical synthesis , Pyrazoles/chemical synthesis , Amides/chemistry , Amides/radiation effects , Aniline Compounds/chemistry , Aniline Compounds/radiation effects , Equipment Design , Microwaves , Miniaturization , Molecular Structure , Piperidines/chemistry , Piperidines/radiation effects , Propionates/chemistry , Propionates/radiation effects , Pyrazoles/chemistry , Pyrazoles/radiation effects , Rimonabant , StereoisomerismABSTRACT
Recent developments in dynamic nuclear polarisation now allow significant enhancements to be generated in the cryo solid state and transferred to the liquid state for detection at high resolution. We demonstrate that the Ardenkjaer-Larsen method can be extended by taking advantage of the properties of the trityl radicals used. It is possible to hyperpolarise 13C and 15N simultaneously in the solid state, and to maintain these hyperpolarisations through rapid dissolution into the liquid state. We demonstrate the almost simultaneous measurement of hyperpolarised 13C and hyperpolarised 15N NMR spectra. The prospects for further improvement of the method using contemporary technology are also discussed.
Subject(s)
Nuclear Magnetic Resonance, Biomolecular/methods , Carbon Isotopes , Free Radicals/chemistry , Free Radicals/radiation effects , Microwaves , Niacinamide/chemistry , Niacinamide/radiation effects , Nitrogen Isotopes , Pyrazoles/chemistry , Pyrazoles/radiation effects , Sensitivity and SpecificityABSTRACT
Nanoparticles of TiO(2) were synthesized by sol-gel technique and the photodeposition of about 1% Ag on TiO(2) particles was carried out. Ag-deposited TiO(2) catalyst was characterised by XRD, TEM and UV-vis spectroscopy. The Ag-TiO(2) catalyst was evaluated for their photocatalytic activity towards the degradation of Reactive Yellow-17 (RY-17) under UV and visible light irradiations. Then the results were compared with synthesized nano-TiO(2) sol and P-25 Degussa and the enhanced degradation was obtained with Ag-deposited TiO(2). This enhanced activity of Ag-TiO(2) may be attributed to the trapping of conduction band electrons. The effect of initial dye concentration, pH and electron acceptors such as H(2)O(2), K(2)S(2)O(8) on the photocatalytic activity were studied and the results obtained were fitted with Langmuir-Hinshelwood model to study the degradation kinetics and discussed in detail.
Subject(s)
Azo Compounds/chemistry , Photolysis , Photosensitizing Agents/chemistry , Pyrazoles/chemistry , Silver/chemistry , Titanium/chemistry , Azo Compounds/radiation effects , Catalysis , Hydrogen Peroxide/chemistry , Hydrogen-Ion Concentration , Kinetics , Potassium Compounds/chemistry , Pyrazoles/radiation effects , Sulfates/chemistry , Ultraviolet RaysABSTRACT
This paper evaluates the quantum yield and electrical energy per order (E(Eo)) efficiency of Reactive Orange 4 (RO4) and Reactive Yellow 14 (RY14) azo dyes by three advanced oxidation processes (AOPs). Both dyes were completely decolourised by all these processes. The relative decolourisation efficiencies of these processes were in the following order: Fe(2+)/H(2)O(2)/UV>UV/TiO(2)>UV/H(2)O(2). The low efficiency of UV/H(2)O(2) process is mainly due to low UV absorption by hydrogen peroxide at the 365nm. The figure of merit E(Eo) values showed that UV/H(2)O(2) process consumes more electrical energy than the other two processes. The electrical energy consumption is in the following order: UV/H(2)O(2)>UV/TiO(2)>Fe(2+)/H(2)O(2)/UV. At low initial dye concentration higher quantum yield was observed in UV/TiO(2) process, whereas in photo-Fenton process higher quantum yield was observed at high initial dye concentration. The structure of dye molecule also influences the quantum yield and E(Eo) value.
Subject(s)
Azo Compounds/chemistry , Azo Compounds/radiation effects , Coloring Agents/chemistry , Coloring Agents/radiation effects , Pyrazoles/chemistry , Pyrazoles/radiation effects , Sulfuric Acid Esters/chemistry , Sulfuric Acid Esters/radiation effects , Color , Electricity , Hydrogen Peroxide/chemistry , Iron/chemistry , Oxidation-Reduction , Titanium/chemistry , Ultraviolet Rays , Water Pollutants, Chemical/chemistry , Water Pollutants, Chemical/radiation effects , Water PurificationABSTRACT
Fipronil is a recently discovered insecticide of the phenylpyrazole series. It has a highly selective biochemical mode of action, which has led to its use in a large number of important agronomical, household, and veterinary applications. Previous studies have shown that, during exposure to light, fipronil is converted into a desulfurated derivative (desulfinyl-fipronil), which has slightly reduced insecticidal activity. In this study, the photodegradation of fipronil was studied in solution at low light intensities (sunlight or UV lamp). In addition to desulfinyl-fipronil, a large number of minor photoproducts were observed, including diversely substituted phenylpyrazole derivatives and aniline derivatives that had lost the pyrazole ring. Desulfinylfipronil itself was shown to be relatively stable under both UV light and sunlight, with only limited changes occurring in the substitution of the aromatic ring. Since this compound accumulated to levels corresponding to only 30-55% of the amount of fipronil degraded, it was concluded that one or more alternative pathways of photodegradation must be operating. On the basis of the structurally identified photoproducts, it is proposed that fipronil photodegradation occurs via at least two distinct pathways, one of which involves desulfuration at the 4-position of the pyrazole ring giving the desulfinyl derivative and the other of which involves a different modification of the 4-substituent, leading to cleavage of the pyrazole ring and the formation of aniline derivatives. The latter compounds do not accumulate to high levels and may, therefore, be degraded further. The ecological significance of these results is discussed, particularly with regard to the insecticidal activity of the photoproducts.
Subject(s)
Insecticides/chemistry , Insecticides/radiation effects , Light , Pyrazoles/chemistry , Pyrazoles/radiation effects , Ultraviolet Rays , Aedes , Aniline Compounds/chemistry , Animals , Gas Chromatography-Mass Spectrometry , Kinetics , Pyrazoles/toxicity , Sulfides/chemistry , Toxicity TestsABSTRACT
Fluoropyridyl derivatives of [3,2-c]pyrazolo-corticosteroids have high affinity for the glucocorticoid receptor (GR) and are highly active glucocorticoids. They are thus considered to be excellent candidates for PET imaging of GR containing tissues when labeled with fluorine-18 (t(1/2)=110 min). Previously reported syntheses of these fluorinated glucocorticoids were accomplished by conventional thermal nucleophilic halogen exchange reactions with chloropyridyl precursors. These reactions were found to proceed at rates too slow for feasible application to radiosynthesis using [(18)F]fluoride. We have applied microwave-heating methods to these reactions and found that significant rate enhancements can be realized. Kinetic experiments showed an average relative rate ratio of 3/1 for microwave versus conventional heating and preparative experiments showed an average relative conversion ratio of 4.5/1 during the initial 120 min, a period approximating one half-life of the isotope. The microwave method described was used to prepare previously unreported 2'-(2-fluoro-4-pyridyl)-11beta,17,21-trihydroxy-16alpha-methyl-20-oxo-pregn-4-eno-[3,2-c]-pyrazole, which was evaluated for biological activity.
Subject(s)
Adrenal Cortex Hormones/chemical synthesis , Fluorine/chemistry , Pregnenes/chemical synthesis , Pyrazoles/chemical synthesis , Pyridines/chemistry , Receptors, Glucocorticoid/drug effects , Adrenal Cortex Hormones/pharmacology , Adrenal Cortex Hormones/radiation effects , Glucocorticoids/chemistry , Glucocorticoids/metabolism , Kinetics , Microwaves , Molecular Structure , Positron-Emission Tomography/methods , Pregnenes/chemistry , Pregnenes/pharmacology , Pyrazoles/chemistry , Pyrazoles/pharmacology , Pyrazoles/radiation effects , Pyridines/radiation effects , Receptors, Glucocorticoid/metabolism , Sensitivity and Specificity , Stereoisomerism , Structure-Activity RelationshipABSTRACT
Microwave irradiation of substituted hydrazines and beta-ketoesters gives 5-aminopyrazoles in excellent yield, which can be transformed to the corresponding N-carbonyl derivatives by treatment with an isocyanate or chloroformate. Derivatization of 4-nitronaphth-1-ol using predominantly microwave heating methods and reaction with an N-pyrazole carbamate provides a rapid route to the N-pyrazole urea BIRB 796 in high purity, as a potent and selective inhibitor of p38alpha mitogen-activated protein kinase for the study of accelerated ageing in Werner syndrome cells.