ABSTRACT
The arginyl-transferase ATE1 is a tRNA-dependent enzyme that covalently attaches an arginine molecule to a protein substrate. Conserved from yeast to humans, ATE1 deficiency in mice correlates with defects in cardiovascular development and angiogenesis and results in embryonic lethality, while conditional knockouts exhibit reproductive, developmental, and neurological deficiencies. Despite the recent revelation of the tRNA binding mechanism and the catalytic cycle of yeast ATE1, the structure-function relationship of ATE1 in higher organisms is not well understood. In this study, we present the three-dimensional structure of human ATE1 in an apo-state and in complex with its tRNA cofactor and a peptide substrate. In contrast to its yeast counterpart, human ATE1 forms a symmetric homodimer, which dissociates upon binding of a substrate. Furthermore, human ATE1 includes a unique and extended loop that wraps around tRNAArg, creating extensive contacts with the T-arm of the tRNA cofactor. Substituting key residues identified in the substrate binding site of ATE1 abolishes enzymatic activity and results in the accumulation of ATE1 substrates in cells.
Subject(s)
Aminoacyltransferases , Protein Multimerization , Humans , Aminoacyltransferases/metabolism , Aminoacyltransferases/genetics , Aminoacyltransferases/chemistry , RNA, Transfer/metabolism , Binding Sites , RNA, Transfer, Arg/metabolism , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/chemistry , Models, Molecular , Protein Binding , Animals , Mice , HEK293 CellsABSTRACT
In this report, non-isomerisable analogs of arginine tRNA (Arg-triazole-tRNA) have been synthesized as tools to study tRNA-dependent aminoacyl-transferases. The synthesis involves the incorporation of 1,4 substituted-1,2,3 triazole ring to mimic the ester bond that connects the amino acid to the terminal adenosine in the natural substrate. The synthetic procedure includes (i) a coupling between 2'- or 3'-azido-adenosine derivatives and a cytidine phosphoramidite to access dinucleotide molecules, (ii) Cu-catalyzed cycloaddition reactions between 2'- or 3'-azido dinucleotide in the presence of an alkyne molecule mimicking the arginine, providing the corresponding Arg-triazole-dinucleotides, (iii) enzymatic phosphorylation of the 5'-end extremity of the Arg-triazole-dinucleotides with a polynucleotide kinase, and (iv) enzymatic ligation of the 5'-phosphorylated dinucleotides with a 23-nt RNA micro helix that mimics the acceptor arm of arg-tRNA or with a full tRNAarg. Characterization of nucleoside and nucleotide compounds involved MS spectrometry, 1H, 13C and 31P NMR analysis. This strategy allows to obtain the pair of the two stable regioisomers of arg-tRNA analogs (2' and 3') which are instrumental to explore the regiospecificity of arginyl transferases enzyme. In our study, a first binding assay of the arg-tRNA micro helix with the Arginyl-tRNA-protein transferase 1 (ATE1) was performed by gel shift assays.
Subject(s)
Copper , Cycloaddition Reaction , Catalysis , Copper/chemistry , Cycloaddition Reaction/methods , Arginine/chemistry , Arginine/analogs & derivatives , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Phosphorylation , Triazoles/chemistry , Triazoles/chemical synthesis , Stereoisomerism , Adenosine/analogs & derivatives , Adenosine/chemistry , Aminoacyltransferases/metabolism , Aminoacyltransferases/chemistry , Aminoacyltransferases/geneticsABSTRACT
This chapter describes the preparation of pre-charged Arg-tRNA that can be used in arginylation reaction. While in a typical arginylation reaction arginyl-tRNA synthetase (RARS) is normally included as a component of the reaction and continually charges tRNA during arginylation, it is sometimes necessary to separate the charging and the arginylation step, in order to perform each reaction under controlled conditions, e.g., for measuring the kinetics or determining the effect of different compounds and chemicals on the reaction. In such cases, tRNAArg can be pre-charged with Arg and purified away from the RARS enzyme prior to arginylation.
Subject(s)
Amino Acyl-tRNA Synthetases , Arginine-tRNA Ligase , Arginine-tRNA Ligase/chemistry , Arginine-tRNA Ligase/genetics , Arginine-tRNA Ligase/metabolism , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Aminoacylation , RNA, Transfer/genetics , Transfer RNA Aminoacylation , Kinetics , Amino Acyl-tRNA Synthetases/metabolismABSTRACT
Posttranslational protein arginylation catalyzed by arginyl transferases is a mechanism to regulate multiple physiological processes. This protein arginylation reaction uses a charged Arg-tRNAArg as the donor of arginine (Arg). The inherent instability of the ester linkage of the arginyl group to the tRNA, which is sensitive to hydrolysis at the physiological pH, makes it difficult to obtain structural information on how the arginyl transfer reaction is catalyzed. Here, we describe a methodology to synthesize stably charged Arg-tRNAArg that would facilitate structural analysis. In the stably charged Arg-tRNAArg, the ester linkage is replaced with an amide linkage, which is resistant to hydrolysis even at alkaline pH.
Subject(s)
Arginine-tRNA Ligase , Arginine , Arginine/metabolism , Arginine-tRNA Ligase/chemistry , Arginine-tRNA Ligase/genetics , Arginine-tRNA Ligase/metabolism , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Protein Binding , RNA, Transfer/metabolismABSTRACT
Colicin D is a plasmid-encoded bacteriocin that specifically cleaves tRNAArg of sensitive Escherichia coli cells. E. coli has four isoaccepting tRNAArgs; the cleavage occurs at the 3' end of anticodon-loop, leading to translation impairment in the sensitive cells. tRNAs form a common L-shaped structure and have many conserved nucleotides that limit tRNA identity elements. How colicin D selects tRNAArgs from the tRNA pool of sensitive E. coli cells is therefore intriguing. Here, we reveal the recognition mechanism of colicin D via biochemical analyses as well as structural modelling. Colicin D recognizes tRNAArgICG, the most abundant species of E. coli tRNAArgs, at its anticodon-loop and D-arm, and selects it as the most preferred substrate by distinguishing its anticodon-loop sequence from that of others. It has been assumed that translation impairment is caused by a decrease in intact tRNA molecules due to cleavage. However, we found that intracellular levels of intact tRNAArgICG do not determine the viability of sensitive cells after such cleavage; rather, an accumulation of cleaved ones does. Cleaved tRNAArgICG dominant-negatively impairs translation in vitro. Moreover, we revealed that EF-Tu, which is required for the delivery of tRNAs, does not compete with colicin D for binding tRNAArgICG, which is consistent with our structural model. Finally, elevation of cleaved tRNAArgICG level decreases the viability of sensitive cells. These results suggest that cleaved tRNAArgICG transiently occupies ribosomal A-site in an EF-Tu-dependent manner, leading to translation impairment. The strategy should also be applicable to other tRNA-targeting RNases, as they, too, recognize anticodon-loops.Abbreviations: mnm5U: 5-methylaminomethyluridine; mcm5s2U: 5-methoxycarbonylmethyl-2-thiouridine.
Subject(s)
Bacteriocins/chemistry , Colicins/chemistry , Escherichia coli/metabolism , Protein Biosynthesis , RNA, Bacterial/chemistry , RNA, Transfer, Arg/chemistry , Ribosomes/metabolism , Anticodon/chemistry , Anticodon/genetics , Anticodon/metabolism , Bacteriocins/genetics , Bacteriocins/metabolism , Base Pairing , Binding Sites , Colicins/genetics , Colicins/metabolism , Escherichia coli/genetics , Gene Expression Regulation, Bacterial , Molecular Docking Simulation , Nucleic Acid Conformation , Peptide Elongation Factor Tu/genetics , Peptide Elongation Factor Tu/metabolism , Plasmids/chemistry , Plasmids/metabolism , Protein Binding , Protein Conformation, alpha-Helical , Protein Conformation, beta-Strand , Protein Interaction Domains and Motifs , RNA, Bacterial/genetics , RNA, Bacterial/metabolism , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Ribosomes/genetics , Substrate Specificity , Thiouridine/analogs & derivatives , Thiouridine/metabolism , Uridine/analogs & derivatives , Uridine/metabolismABSTRACT
In mammals, a subset of arginine tRNA isoacceptors are methylated in the anticodon loop by the METTL2 methyltransferase to form the 3-methylcytosine (m3C) modification. However, the mechanism by which METTL2 identifies specific tRNA arginine species for m3C formation as well as the biological role of m3C in mammals is unknown. Here, we show that human METTL2 forms a complex with DALR anticodon binding domain containing 3 (DALRD3) protein to recognize particular arginine tRNAs destined for m3C modification. DALRD3-deficient human cells exhibit nearly complete loss of the m3C modification in tRNA-Arg species. Notably, we identify a homozygous nonsense mutation in the DALRD3 gene that impairs m3C formation in human patients exhibiting developmental delay and early-onset epileptic encephalopathy. These findings uncover an unexpected function for the DALRD3 protein in the targeting of distinct arginine tRNAs for m3C modification and suggest a crucial biological role for DALRD3-dependent tRNA modification in proper neurological development.
Subject(s)
Cytosine/analogs & derivatives , Epilepsy/metabolism , RNA, Transfer, Arg/metabolism , tRNA Methyltransferases/metabolism , Age of Onset , Cell Line , Cytosine/metabolism , Epilepsy/genetics , Humans , Nucleic Acid Conformation , Protein Binding , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , tRNA Methyltransferases/geneticsABSTRACT
Adenosine deaminase acting on transfer RNA (ADAT) is an essential eukaryotic enzyme that catalyzes the deamination of adenosine to inosine at the first position of tRNA anticodons. Mammalian ADATs modify eight different tRNAs, having increased their substrate range from a bacterial ancestor that likely deaminated exclusively tRNAArg Here we investigate the recognition mechanisms of tRNAArg and tRNAAla by human ADAT to shed light on the process of substrate expansion that took place during the evolution of the enzyme. We show that tRNA recognition by human ADAT does not depend on conserved identity elements, but on the overall structural features of tRNA. We find that ancestral-like interactions are conserved for tRNAArg, while eukaryote-specific substrates use alternative mechanisms. These recognition studies show that human ADAT can be inhibited by tRNA fragments in vitro, including naturally occurring fragments involved in important regulatory pathways.
Subject(s)
Adenosine Deaminase/metabolism , Anticodon/chemistry , RNA, Transfer, Ala/chemistry , RNA, Transfer, Arg/chemistry , Adenosine/metabolism , Adenosine Deaminase/genetics , Anticodon/genetics , Anticodon/metabolism , Base Sequence , Deamination , Evolution, Molecular , Gene Expression , Humans , Inosine/metabolism , Nucleic Acid Conformation , RNA, Transfer, Ala/genetics , RNA, Transfer, Ala/metabolism , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Sequence Alignment , Substrate SpecificityABSTRACT
Inosine at the "wobble" position (I34) is one of the few essential posttranscriptional modifications in tRNAs (tRNAs). It results from the deamination of adenosine and occurs in bacteria on tRNAArgACG and in eukarya on six or seven additional tRNA substrates. Because inosine is structurally a guanosine analogue, reverse transcriptases recognize it as a guanosine. Most methods used to examine the presence of inosine rely on this phenomenon and detect the modified base as a change in the DNA sequence that results from the reverse transcription reaction. These methods, however, cannot always be applied to tRNAs because reverse transcription can be compromised by the presence of other posttranscriptional modifications. Here we present SL-ID (splinted ligation-based inosine detection), a reverse transcription-free method for detecting inosine based on an I34-dependent specific cleavage of tRNAs by endonuclease V, followed by a splinted ligation and polyacrylamide gel electrophoresis analysis. We show that the method can detect I34 on different tRNA substrates and can be applied to total RNA derived from different species, cell types, and tissues. Here we apply the method to solve previous controversies regarding the modification status of mammalian tRNAArgACG.
Subject(s)
Deoxyribonuclease IV (Phage T4-Induced)/chemistry , Electrophoresis, Polyacrylamide Gel/methods , Inosine/analysis , Oligodeoxyribonucleotides/chemistry , RNA, Transfer, Arg/chemistry , RNA, Transfer, Val/chemistry , Animals , Base Sequence , HEK293 Cells , HeLa Cells , Humans , Inosine/genetics , Mice , Nucleic Acid Hybridization , Oligodeoxyribonucleotides/genetics , RNA, Transfer, Arg/genetics , RNA, Transfer, Val/geneticsABSTRACT
Aminoacyl-tRNA synthetases are essential components in protein biosynthesis. Arginyl-tRNA synthetase (ArgRS) belongs to the small group of aminoacyl-tRNA synthetases requiring cognate tRNA for amino acid activation. The crystal structure of Escherichia coli (Eco) ArgRS has been solved in complex with tRNAArg at 3.0-Å resolution. With this first bacterial tRNA complex, we are attempting to bridge the gap existing in structure-function understanding in prokaryotic tRNAArg recognition. The structure shows a tight binding of tRNA on the synthetase through the identity determinant A20 from the D-loop, a tRNA recognition snapshot never elucidated structurally. This interaction of A20 involves 5 amino acids from the synthetase. Additional contacts via U20a and U16 from the D-loop reinforce the interaction. The importance of D-loop recognition in EcoArgRS functioning is supported by a mutagenesis analysis of critical amino acids that anchor tRNAArg on the synthetase; in particular, mutations at amino acids interacting with A20 affect binding affinity to the tRNA and specificity of arginylation. Altogether the structural and functional data indicate that the unprecedented ArgRS crystal structure represents a snapshot during functioning and suggest that the recognition of the D-loop by ArgRS is an important trigger that anchors tRNAArg on the synthetase. In this process, A20 plays a major role, together with prominent conformational changes in several ArgRS domains that may eventually lead to the mature ArgRS:tRNA complex and the arginine activation. Functional implications that could be idiosyncratic to the arginine identity of bacterial ArgRSs are discussed.
Subject(s)
Arginine-tRNA Ligase/chemistry , Arginine-tRNA Ligase/metabolism , Escherichia coli Proteins/chemistry , Escherichia coli Proteins/metabolism , Escherichia coli/metabolism , Ligases/chemistry , Ligases/metabolism , RNA, Transfer, Arg/metabolism , Arginine-tRNA Ligase/genetics , Binding Sites , Crystallography, X-Ray , Escherichia coli/chemistry , Escherichia coli/genetics , Escherichia coli Proteins/genetics , Ligases/genetics , Models, Molecular , Mutagenesis, Site-Directed , Protein Binding , Protein Conformation , RNA, Bacterial , RNA, Transfer, Arg/chemistryABSTRACT
Excess cellular heme is toxic, and malaria parasites regulate its levels during hemoglobin digestion. Aminoacyl-tRNA synthetases are ubiquitous enzymes, and of these, arginyl-tRNA synthetase (RRS) is unique as its enzymatic product of charged tRNA is required for protein synthesis and degradation. We show that Plasmodium falciparum arginyl-tRNA synthetase (PfRRS) is an active, cytosolic, and monomeric enzyme. Its high-resolution crystal structure highlights critical structural differences with the human enzyme. We further show that hemin binds to and inhibits the aminoacylation activity of PfRRS. Hemin induces a dimeric form of PfRRS that is thus rendered enzymatically dead as it is unable to recognize its cognate tRNA(arg). Excessive hemin in chloroquine-treated malaria parasites results in significantly reduced charged tRNA(arg) levels, thus suggesting deceleration of protein synthesis. These data together suggest that the inhibition of Plasmodium falciparum arginyl-tRNA synthetase can now be synergized with existing antimalarials for more potent drug cocktails against malaria parasites.
Subject(s)
Arginine-tRNA Ligase/chemistry , Arginine/chemistry , Heme/chemistry , Hemin/chemistry , Plasmodium falciparum/drug effects , Protozoan Proteins/chemistry , RNA, Transfer, Arg/chemistry , Amino Acid Sequence , Antimalarials/chemistry , Antimalarials/pharmacology , Arginine/metabolism , Arginine-tRNA Ligase/genetics , Arginine-tRNA Ligase/metabolism , Binding Sites , Chloroquine/chemistry , Chloroquine/pharmacology , Crystallography, X-Ray , Gene Expression , Heme/pharmacology , Hemin/pharmacology , Humans , Models, Molecular , Plasmodium falciparum/enzymology , Plasmodium falciparum/genetics , Plasmodium falciparum/growth & development , Protein Binding , Protein Biosynthesis/drug effects , Protein Interaction Domains and Motifs , Protein Multimerization , Protein Structure, Secondary , Protozoan Proteins/genetics , Protozoan Proteins/metabolism , RNA, Transfer, Arg/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Species Specificity , Substrate SpecificityABSTRACT
Fitness landscapes describe the genotype-fitness relationship and represent major determinants of evolutionary trajectories. However, the vast genotype space, coupled with the difficulty of measuring fitness, has hindered the empirical determination of fitness landscapes. Combining precise gene replacement and next-generation sequencing, we quantified Darwinian fitness under a high-temperature challenge for more than 65,000 yeast strains, each carrying a unique variant of the single-copy tRNA(CCU)(Arg) gene at its native genomic location. Approximately 1% of single point mutations in the gene were beneficial and 42% were deleterious. Almost half of all mutation pairs exhibited statistically significant epistasis, which had a strong negative bias, except when the mutations occurred at Watson-Crick paired sites. Fitness was broadly correlated with the predicted fraction of correctly folded transfer RNA (tRNA) molecules, thereby revealing a biophysical basis of the fitness landscape.
Subject(s)
Genes, Fungal , Genetic Fitness , RNA Folding , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , Saccharomyces cerevisiae/genetics , Anticodon/chemistry , Anticodon/genetics , Base Pairing , DNA Mutational Analysis , Epistasis, Genetic , Evolution, Molecular , Gene Dosage , Gene Expression Regulation, Fungal , Hot Temperature , Point MutationABSTRACT
The efficiency of the cellular oxidative phosphorylation system was recently shown to be modulated by common mitochondrial tRNA(A) (rg) haplotypes. The molecular mechanism by which some mt-Tr haplotypes induce these functional differences remains undetermined. Common polymorphisms in mouse mt-Tr genes affect the size of the dihydrouridine loop in the mature tRNA, producing loops of between five and seven nucleotides, the largest being a rare variant among mammals. Here, we analyzed a new mt-Tr variant identified in C3H mice, and found that it is mitochondrial tRNA loop size, but not the specific sequence, that is responsible for the observed differences in cellular respiration. We further found that the sensitivity of mitochondrial protein synthesis to specific inhibitors is dependent on the mt-Tr gene haplotype, and confirmed that the differences in oxidative phosphorylation performance are masked by a reactive oxygen species-induced compensatory increase in mitochondrial biogenesis.
Subject(s)
RNA, Transfer, Arg/genetics , RNA/genetics , Animals , Cell Division , Galactose/metabolism , Haplotypes , Mice , Mice, Inbred C3H , Mice, Inbred C57BL , Mitochondria/metabolism , NIH 3T3 Cells , Oxidative Phosphorylation , RNA/chemistry , RNA/metabolism , RNA, Mitochondrial , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/metabolism , Reactive Oxygen Species/metabolismABSTRACT
In most bacteria, two tRNAs decode the four arginine CGN codons. One tRNA harboring a wobble inosine (tRNA(Arg)ICG) reads the CGU, CGC and CGA codons, whereas a second tRNA harboring a wobble cytidine (tRNA(Arg)CCG) reads the remaining CGG codon. The reduced genomes of Mycoplasmas and other Mollicutes lack the gene encoding tRNA(Arg)CCG. This raises the question of how these organisms decode CGG codons. Examination of 36 Mollicute genomes for genes encoding tRNA(Arg) and the TadA enzyme, responsible for wobble inosine formation, suggested an evolutionary scenario where tadA gene mutations first occurred. This allowed the temporary accumulation of non-deaminated tRNA(Arg)ACG, capable of reading all CGN codons. This hypothesis was verified in Mycoplasma capricolum, which contains a small fraction of tRNA(Arg)ACG with a non-deaminated wobble adenosine. Subsets of Mollicutes continued to evolve by losing both the mutated tRNA(Arg)CCG and tadA, and then acquired a new tRNA(Arg)UCG. This permitted further tRNA(Arg)ACG mutations with tRNA(Arg)GCG or its disappearance, leaving a single tRNA(Arg)UCG to decode the four CGN codons. The key point of our model is that the A-to-I deamination activity had to be controlled before the loss of the tadA gene, allowing the stepwise evolution of Mollicutes toward an alternative decoding strategy.
Subject(s)
Adenosine Deaminase/genetics , Codon , Evolution, Molecular , Mycoplasma/genetics , RNA, Transfer, Arg/genetics , Tenericutes/genetics , Adenosine/metabolism , Adenosine Deaminase/chemistry , Amino Acid Sequence , Arginine/metabolism , Deamination , Molecular Sequence Data , Mycoplasma/enzymology , Mycoplasma capricolum/genetics , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/metabolism , Sequence Alignment , Tenericutes/enzymologyABSTRACT
It is a prevalent concept that, in line with the Wobble Hypothesis, those tRNAs having an adenosine in the first position of the anticodon become modified to an inosine at this position. Sequencing the cDNA derived from the gene coding for cytoplasmic tRNA (Arg) ACG from several higher plants as well as mass spectrometric analysis of the isoacceptor has revealed that for this kingdom an unmodified A in the wobble position of the anticodon is the rule rather than the exception. In vitro translation shows that in the plant system the absence of inosine in the wobble position of tRNA (Arg) does not prevent decoding. This isoacceptor belongs to the class of tRNA that is imported from the cytoplasm into the mitochondria of higher plants. Previous studies on the mitochondrial tRNA pool have demonstrated the existence of tRNA (Arg) ICG in this organelle. In moss the mitochondrial encoded distinct tRNA (Arg) ACG isoacceptor possesses the I34 modification. The implication is that for mitochondrial protein biosynthesis A-to-I editing is necessary and occurs by a mitochondrion-specific deaminase after import of the unmodified nuclear encoded tRNA (Arg) ACG.
Subject(s)
Adenosine/metabolism , Anticodon/metabolism , Glycine max/genetics , Inosine/metabolism , Protein Biosynthesis , RNA, Transfer, Arg/metabolism , Triticum/genetics , Adenosine/genetics , Adenosine Deaminase/metabolism , Anticodon/chemistry , Anticodon/genetics , Base Pairing , Base Sequence , Cell Nucleus/genetics , Cell Nucleus/metabolism , Cell-Free System , Cytoplasm/genetics , Cytoplasm/metabolism , Escherichia coli/genetics , Escherichia coli/metabolism , Genetic Code , Inosine/genetics , Mitochondria/genetics , Mitochondria/metabolism , Molecular Sequence Data , Nucleic Acid Conformation , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , Glycine max/metabolism , Sphagnopsida/genetics , Sphagnopsida/metabolism , Triticum/metabolismABSTRACT
Three of six arginine codons are read by two tRNA(Arg) isoacceptors in Escherichia coli. The anticodon stem and loop of these isoacceptors (ASL(Arg1,2)) differs only in that the position 32 cytidine of tRNA(Arg1) is posttranscriptionally modified to 2-thiocytidine (s(2)C(32)). The tRNA(Arg1,2) are also modified at positions 34 (inosine, I(34)) and 37 (2-methyladenosine, m(2)A(37)). To investigate the roles of modifications in the structure and function, we analyzed six ASL(Arg1,2) constructs differing in their array of modifications by spectroscopy and codon binding assays. Thermal denaturation and circular dichroism spectroscopy indicated that modifications contribute thermodynamic and base stacking properties, resulting in more order but less stability. NMR-derived structures of the ASL(Arg1,2) showed that the solution structures of the ASLs were nearly identical. Surprisingly, none possessed the U-turn conformation required for effective codon binding on the ribosome. Yet, all ASL(Arg1,2) constructs efficiently bound the cognate CGU codon. Three ASLs with I(34) were able to decode CGC, whereas only the singly modified ASL(Arg1,2)(ICG) with I(34) was able to decode CGA. The dissociation constants for all codon bindings were physiologically relevant (0.4-1.4 µM). However, with the introduction of s(2)C(32) or m(2)A(37) to ASL(Arg1,2)(ICG), the maximum amount of ASL bound to CGU and CGC was significantly reduced. These results suggest that, by allowing loop flexibility, the modifications modulate the conformation of the ASL(Arg1,2), which takes one structure free in solution and two others when bound to the cognate arginyl-tRNA synthetase or to codons on the ribosome where modifications reduce or restrict binding to specific codons.
Subject(s)
Anticodon/chemistry , Codon/chemistry , Escherichia coli/metabolism , RNA, Transfer, Arg/chemistry , Base Pairing , Binding Sites , Circular Dichroism , Crystallography, X-Ray , Escherichia coli/genetics , Escherichia coli Proteins/chemistry , Nucleic Acid Conformation , Protein Conformation , Protein Isoforms/chemistry , Protein Processing, Post-Translational , ThermodynamicsABSTRACT
There is increasing awareness of the role of mtDNA alterations in the development of cancer, as mtDNA point mutations are found at high frequency in a variety of human tumors. To determine the biological effects of mtDNA mutations in UV-induced skin tumors, hairless mice were irradiated to produce tumors, and the tumor mtDNAs were screened for single-nucleotide changes using temperature gradient capillary electrophoresis (TGCE), followed by direct sequencing. A mutation hot spot (9821insA) in the mitochondrially encoded tRNA arginine (mt-Tr) locus (tRNA(Arg)) was discovered in approximately one-third of premalignant and malignant skin tumors. To determine the functional relevance of this particular mutation in vitro, cybrid cell lines containing different mt-Tr (tRNA(Arg)) alleles were generated. The resulting cybrid cell lines contained the same nuclear genotype and differed only in their mtDNAs. The biochemical analysis of the cybrids revealed that the mutant haplotype is associated with diminished levels of complex I protein (CI), resulting in lower levels of baseline oxygen consumption and lower cellular adenosine triphosphate (ATP) production. We hypothesize that this specific mtDNA mutation alters cellular biochemistry, supporting the development of keratinocyte neoplasia.
Subject(s)
DNA, Mitochondrial/genetics , Mutation , Neoplasms, Radiation-Induced/genetics , Skin Neoplasms/genetics , Adenosine Triphosphate/biosynthesis , Animals , Female , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Neoplasms, Radiation-Induced/metabolism , Oxygen Consumption , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , Skin Neoplasms/metabolism , Ultraviolet RaysABSTRACT
In higher eukaryotes, the gene family encoding the 5S ribosomal RNA (5S rRNA) has been used (together with histones) to showcase the archetypal example of a gene family subject to concerted evolution. However, recent studies have revealed conspicuous features challenging the predictions of this model, including heterogeneity of repeat units, the presence of functional 5S gene variants as well as the existence of 5S rDNA divergent pseudogenes lacking traces of homogenization. In the present work, we have broadened the scope in the evolutionary study of ribosomal gene families by studying the 5S rRNA family in mussels, a model organism which stands out among other animals due to the heterogeneity it displays regarding sequence and organization. To this end, 48 previously unknown 5S rDNA units (coding and spacer regions) were sequenced in five mussel species, leading to the characterization of two new types of units (referred to here as small-beta 5S rDNA and gamma-5S rDNA) coexisting in the genome with alpha and beta rDNA units. The intense genetic dynamics of this family is further supported by the first description of an association between gamma-5S rDNA units and tRNA genes. Molecular evolutionary and phylogenetic analyses revealed an extensive lack of homology among spacer sequences belonging to different rDNA types, suggesting the presence of independent evolutionary pathways leading to their differentiation. Overall, our results suggest that the long-term evolution of the 5S rRNA gene family in mussels is most likely mediated by a mixed mechanism involving the generation of genetic diversity through birth-and-death, followed by a process of local homogenization resulting from concerted evolution in order to maintain the genetic identities of the different 5S units, probably after their transposition to independent chromosomal locations.
Subject(s)
Evolution, Molecular , Mytilus/genetics , RNA, Ribosomal, 5S/genetics , Animals , Cluster Analysis , DNA/chemistry , DNA/genetics , Nucleic Acid Conformation , Phylogeny , Polymorphism, Genetic , Promoter Regions, Genetic , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/geneticsABSTRACT
Colicin D is a plasmid-encoded antibacterial protein that specifically cleaves the anticodon loops of four Escherichia coli tRNA(Arg) species. Here, we report that the catalytic domain of colicin D, which is expressed in Saccharomyces cerevisiae, impairs cell growth by cleaving specific tRNAs. DNA microarray analysis revealed that mating-related genes were upregulated, while genes involved in a range of metabolic processes were downregulated, thereby impairing cell growth. The pheromone-signalling pathway was activated only in alpha cells by tRNA cleavage, which was not observed in 'a' cells or diploid cells. On the basis of these results and on the recent identification of two killer toxins that cleave specific tRNAs, the relationship between tRNA depletion and the resultant cellular response is discussed.
Subject(s)
Escherichia coli Proteins/metabolism , RNA, Fungal/metabolism , RNA, Transfer/metabolism , Saccharomyces cerevisiae/metabolism , Base Sequence , Cell Proliferation , Cytosol/metabolism , Escherichia coli Proteins/chemistry , Escherichia coli Proteins/genetics , Nucleic Acid Conformation , Oligonucleotide Array Sequence Analysis , Peptide Fragments/chemistry , Peptide Fragments/genetics , Peptide Fragments/metabolism , Pheromones/metabolism , Plasmids/genetics , Protein Structure, Tertiary , RNA, Bacterial/chemistry , RNA, Bacterial/genetics , RNA, Bacterial/metabolism , RNA, Fungal/chemistry , RNA, Fungal/genetics , RNA, Transfer/chemistry , RNA, Transfer/genetics , RNA, Transfer, Arg/chemistry , RNA, Transfer, Arg/genetics , RNA, Transfer, Arg/metabolism , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Saccharomyces cerevisiae/genetics , Saccharomyces cerevisiae/growth & development , Saccharomyces cerevisiae Proteins/metabolism , Signal Transduction , Transcription, Genetic , Transformation, GeneticABSTRACT
RNA editing changes the coding/decoding information relayed by transcripts via nucleotide insertion, deletion, or conversion. Editing of tRNA anticodons by deamination of adenine to inosine is used both by eukaryotes and prokaryotes to expand the decoding capacity of individual tRNAs. This limits the number of tRNA species required for codon-anticodon recognition. We have identified the Arabidopsis thaliana gene that codes for tRNA adenosine deaminase arginine (TADA), a chloroplast tRNA editing protein specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg(ICG). Land plant TADAs have a C-terminal domain similar in sequence and predicted structure to prokaryotic tRNA deaminases and also have very long N-terminal extensions of unknown origin and function. Biochemical and mutant complementation studies showed that the C-terminal domain is sufficient for cognate tRNA deamination both in vitro and in planta. Disruption of TADA has profound effects on chloroplast translation efficiency, leading to reduced yields of chloroplast-encoded proteins and impaired photosynthetic function. By contrast, chloroplast transcripts accumulate to levels significantly above those of wild-type plants. Nevertheless, absence of cp-tRNAArg(ICG) is compatible with plant survival, implying that two out of three CGN codon recognition occurs in chloroplasts, though this mechanism is less efficient than wobble pairing.