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1.
Int. j. morphol ; 40(4): 966-972, 2022. ilus, tab
Article in English | LILACS | ID: biblio-1405250

ABSTRACT

SUMMARY: Plastination is an anatomical preparate preparation technique characterized by the replacement of tissue fluids with a reactive polymer. Although more challenging and economically costly than many anatomical methods, this method is desirable because of the fact that specimens created in this method are highly similar to the natural appearance of the intended objects, and they are durable and harmless end products for human health. Our main goal was to completely leave out formaldehyde and similar carcinogenic chemicals used in a method like plastination and to allow production of formaldehyde-free plastinates to be used in anatomy training and examinations in our country. To that end, we compared nose and tongue of 10 large ruminants by subjecting them to plastination, 5 of them with formaldehyde and 5 of them without formaldehyde, and aimed to leave formaldehyde out by taking into account the difference between them. Silicone plastination is the most commonly-used and best-known technique among the plastination techniques because specimens created using this technique look aesthetically impressive. Silicone plastination consists mainly of 5 phases. First of all, we obtained the anatomical situs we wanted and made specimens ready by dissecting some of them after fixation and some of them without fixation. Then, after the implementation of a dehydration phase in acetone baths at -25 °C, a forced impregnation phase was implemented by using a mixture of S10-S3 chemical under negative pressure. In the final phase, the curing and hardening phase, the plastination process was completed by giving the specimens their final shape with the use of the S6 solution. As a result, no significant difference was observed between silicone plastination with and without formaldehyde.


RESUMEN: La plastinación es una técnica de preparados anatómicos caracterizada por la sustitución de fluidos tisulares por un polímero reactivo. A pesar de ser económicamente más costoso que muchas métodos anatómicos, este técnica es deseable debido a que las muestras creadas son muy similares a la apariencia natural de los objetos previstos y son productos finales duraderos e inofensivos para la salud humana. Nuestro objetivo principal fue dejar completamente de lado el formaldehído y las sustancias químicas cancerígenas similares utilizadas en un método como la plastinación y permitir la producción de plastinados libres de formaldehído para su uso en la formación y los exámenes de anatomía en nuestro país. Con ese fin, comparamos la nariz y la lengua de 10 rumiantes mayores sometiéndolos a plastinación, 5 de ellos con formaldehído y 5 de ellos sin formaldehído, y buscamos eliminar el formaldehído considerando la diferencia entre ellos. La plastinación con silicona es la técnica más utilizada y más conocida entre las técnicas de plastinación porque los especímenes creados con ella se ven estéticamente impresionantes. La plastinación con silicona consta principalmente de 5 fases. En primer lugar, obtuvimos el situs anatómico que queríamos y preparamos los especímenes diseccionando algunos de ellos después de la fijación y otros sin fijación. Luego, de la implementación de una fase de deshidratación en baños de acetona a -25 °C, se implementó una fase de impregnación forzada utilizando una mezcla del químico S10-S3 a presión negativa. En la fase final, la fase de curado y endurecimiento, se completó el proceso de plastinación dando a los especímenes su forma definitiva con el uso de la solución S6. Como resultado, no se observaron diferencias significativas entre la plastinación con silicona con y sin formaldehído.


Subject(s)
Animals , Silicones/chemistry , Ruminants/anatomy & histology , Formaldehyde/chemistry , Plastination/methods , Acetone
2.
Int. j. morphol ; 39(3): 759-765, jun. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1385410

ABSTRACT

SUMMARY: The histo-morphology and biometry of liver, lung and kidney of goat were studied. The obtained samples were divided into two groups. One set of tissue was processed for paraffin embedding after formalin fixation. The other set was indigenously plastinated. The plastinates were embedded with paraffin without deplastination. Both the non plastinated and plastinated tissues were sectioned and processed for routine staining. The tissues were examined under light microscope for histological architectures and quantitatively assessed the biometric parameters. The non plastinated and plastinated samples yielded mostly similar histological architectures. But plastinated liver showed alterations and artifacts with enlargement of the central vein and hepatic sinusoidal space. Plastinated lung revealed enlarged alveolar sac. Lack of nuclear clarity was observed for all the plastinated samples. The plastinated kidney revealed compactness of the cellular structures and shrinkage induced artifacts with clear renal corpuscles structure and obvious glomerular capsule (Bowman's capsule). The biometric measurements of central vein of liver, alveolar sac of lung, glomerulus and glomerular space of kidney of both plastinated and non plastinated tissue were also performed using calibrated stage micrometer. Comparison was done between the biometric data for both plastinated and non plastinated tissue. No significant difference was found in the obtained biometric data of liver and lung. Only a significant difference was observed between the width of glomerulus of non plastinated kidney and width of glomerulus of plastinated kidney. The morphological and biometric study of non plastinated and plastinated organs of goat can conclude that histological slides can be made from indigenously plastinated organs with well preserved histological architectures of the tissues with some rare exceptions. Plastination does not affect the biometric characteristics of the organs. In addition, the comparative morphologic and biometric study of plastinated and non plastinated organs of goat will be useful in education and research.


RESUMEN: En este estudio se analizó la histomorfología y biometría de hígado, pulmón y riñón de cabra. Las muestras obtenidas se dividieron en dos grupos: una cohorte de tejido fue procesada para su inclusión en parafina después de la fijación con formalina. La siguiente cohorte fue plastinada localmente. Tanto los tejidos no plastinados como los plastinados fueron seccionados y procesados para tinción de rutina. Los tejidos se examinaron con un microscopio óptico en busca de arquitecturas histológicas y se evaluaron cuantitativamente los parámetros biométricos. Las muestras no plastinadas y plastinadas produjeron arquitecturas histológicas en su mayoría similares. El hígado plastinado mostró alteraciones y artefactos con un aumento de la vena central y del espacio sinusoidal hepático. El pulmón plastinado reveló aumento del saco alveolar. Se observó falta de claridad nuclear en todas las muestras plastinadas. El riñón plastinado reveló compacidad de las estructuras celulares y artefactos inducidos por contracción con estructura clara de corpúsculos renales y obvia cápsula glomerular (cápsula de Bowman). Las mediciones biométricas de la vena central del hígado, el saco alveolar del pulmón, el glomérulo y el espacio glomerular del riñón de tejido plastinado y no plastinado también se realizaron utilizando un micrómetro de platina calibrado. Se realizó una comparación entre los datos biométricos del tejido plastinado y no plastinado. No se encontraron diferencias significativas en los datos biométricos obtenidos de hígado y pulmón. Se observó una diferencia significativa entre el ancho del glomérulo del riñón no plastinado y el ancho del glomérulo del riñón plastinado. En el estudio morfológico y biométrico de órganos de cabra no plastinados y plastinados se puede concluir que es posible hacer portaobjetos histológicos a partir de órganos plastinados naturales con arquitecturas histológicas de los tejidos bien conservadas, con algunas excepciones. La plastinación no afecta las características biométricas de los órganos. Además, el estudio comparativo morfológico y biométrico de órganos plastinados y no plastinados de cabra será de utilidad en la educación y la investigación.


Subject(s)
Animals , Goats/anatomy & histology , Plastination , Ruminants/anatomy & histology , Biometry , Kidney/anatomy & histology , Liver/anatomy & histology , Lung/anatomy & histology
3.
Int. j. morphol ; 39(2): 423-429, abr. 2021. ilus, tab
Article in English | LILACS | ID: biblio-1385344

ABSTRACT

SUMMARY: The Saanen goat is known as the greatest milk producer among small ruminat breeds. However, its morphometric features still remain unclear. Therefore, the present work aimed to investigate the functional anatomy of the upper and lower jaws as well as the volumetric properties of the male and female Saanen goat for clinical applications. The heads of 20 adult animals (10 males and 10 females) were included. Totally, 22 morphometric parameters were measured on three dimensional computed tomographic images using RadiAnt DICOM Viewer software and some parameters were measured on hot macerated sample. The mean volume of paranasal sinuses as well as conchal sinuses were estimated using stereological method. Based on the results, the differences between males and females were not significant (p>0.05) in all desired parameters.The frontal and lacrimal sinuses were the largest and smallest paranasal sinus in both sexes. Also, the dorsal and middle conchal sinuses were the largest and smallest ones, respectively. The common nasal meatus was the smallest and ventral meatus was the largest meatus in the nasal cavity. In conclusion, these findings provide a basic data that would be useful in blocking terminal branches of the cranial nerves in this breed for surgical purpose or teeth injuries treatment.


RESUMEN: La cabra Saanen es conocida como la mayor productora de leche entre las razas de pequeños ruminos, sin embargo, sus características morfométricas aún permanecen sin revelar. Por lo tanto, el presente trabajo tuvo como objetivo investigar la anatomía de los huesos del cráneo y cara, así como sus propiedades volumétricas, en la cabra Saanen, tanto del macho como de la hembra con la finalidad de contribuir con las aplicaciones clínicas. Se incluyeron las cabezas de 20 animales adultos (10 machos y 10 hembras). Se midieron 22 parámetros morfométricos en imágenes de tomografía computarizada tridimensionales utilizando el software RadiAnt DICOM Viewer; algunos parámetros se midieron en muestra macerada en calor. El volumen medio de los senos paranasales y concales se estimó mediante método estereológico. En base a los resultados, las diferencias entre machos y hembras no fueron significativas (p> 0.05) en todos los parámetros deseados. Los senos frontal y lagrimal eran de mayor y menor volumen en ambos sexos, respectivamente. Además, los senos conchal dorsal y medio eran los más grandes y los más pequeños, respectivamente. El meato nasal común fue el más pequeño y el meato ventral el más grande en la cavidad nasal. En conclusión, estos hallazgos brindan un dato básico que sería útil en el bloqueo de las ramas terminales de los nervios craneales en esta raza, con fines quirúrgicos o tratamiento de lesiones dentales.


Subject(s)
Animals , Male , Female , Skull/anatomy & histology , Goats/anatomy & histology , Skull/diagnostic imaging , Ruminants/anatomy & histology , Tomography, X-Ray Computed , Anesthesiology , Microscopy
4.
Acta Sci. Anim. Sci. ; 43: e51265, ago. 2021. graf
Article in English | VETINDEX | ID: vti-32332

ABSTRACT

Feeding behavior analysis provides information about the relationships between animals and pastures. Therefore, this review aims to describe some aspects of the feeding behavior profiles of both sheep and goats in grazing systems. The structure of the pasture is a key factor in the feeding behavior of grazing animals. The amount of feed consumed in a given period of time is affected by the number of meals, duration and velocity of swallowing, changes in grazing time, bite rate, bite weight, and quality of ingested forage. The different phenological stages of forage also influence the animals strategies to optimize their intake, which consequently changes their behavioral activities. Sheep and goats tend to be more selective than cattle, and young animals are more selective than older animals; this selectivity characteristicis one of the most important aspects to be observed in pasture management. According to the degree of selectivity, the animals will intake forages of higher or lower nutritive quality. In addition, the intensity and distribution of their daily activities (grazing, ruminating, and resting)are influenced by several factors, such as the availability and nutritive value of the pasture, its management, the animal activity in the group, and the predominant climatic conditions of the region.(AU)


Subject(s)
Animals , Sheep/anatomy & histology , Sheep/growth & development , Ruminants/anatomy & histology , Ruminants/growth & development , Pasture/analysis , Feeding Behavior
5.
Acta Sci. Anim. Sci. ; 43: e52881, ago. 2021. tab
Article in English | VETINDEX | ID: vti-32214

ABSTRACT

Data analysis in goat production, such as those related to body and scrotal measurements, indicate the productive and reproductive animal development. The current study aimed to evaluate the correlations between thoracic perimeter (TP), body length (BL), body compacity (BC), body volume (BV), and scrotal circumference (SC) with body weight (BW) in young male goats of Saanen and Boer breeds. It was used 38 Saanen and 24 Boer male goats, with age average of 7.2 ± 2.0 months. Thoracic perimeter and body length measurements were obtained using a tape measure (cm) and the live weight (kg) a mechanic scale. The variables body compacity (BC) and body volume (BV) were calculated using the equations: and . Boer breed showed live weight and body compacity higher than Saanen breed (p < 0.05). Regarding correlations between biometric measurements and body weight, we did not find any statistical differences between the breeds (p > 0.05). The scrotal circumference presented the lowest association with body weight (p < 0.05). However, all biometric measurements showed highly significant correlations with live body (p < 0.01). In conclusion, thoracic perimeter was the main measure of body weight predictor, considering efficiency and practical aspects.(AU)


Subject(s)
Animals , Ruminants/anatomy & histology , Ruminants/growth & development , Body Weights and Measures/veterinary , Biometry , Body Weight
6.
Acta sci., Anim. sci ; 43: e52881, 2021. tab
Article in English | VETINDEX | ID: biblio-1459987

ABSTRACT

Data analysis in goat production, such as those related to body and scrotal measurements, indicate the productive and reproductive animal development. The current study aimed to evaluate the correlations between thoracic perimeter (TP), body length (BL), body compacity (BC), body volume (BV), and scrotal circumference (SC) with body weight (BW) in young male goats of Saanen and Boer breeds. It was used 38 Saanen and 24 Boer male goats, with age average of 7.2 ± 2.0 months. Thoracic perimeter and body length measurements were obtained using a tape measure (cm) and the live weight (kg) a mechanic scale. The variables body compacity (BC) and body volume (BV) were calculated using the equations: and . Boer breed showed live weight and body compacity higher than Saanen breed (p 0.05). The scrotal circumference presented the lowest association with body weight (p < 0.05). However, all biometric measurements showed highly significant correlations with live body (p < 0.01). In conclusion, thoracic perimeter was the main measure of body weight predictor, considering efficiency and practical aspects.


Subject(s)
Animals , Biometry , Body Weights and Measures/veterinary , Ruminants/anatomy & histology , Ruminants/growth & development , Body Weight
7.
Acta sci., Anim. sci ; 43: e51265, 2021. graf
Article in English | VETINDEX | ID: biblio-1459988

ABSTRACT

Feeding behavior analysis provides information about the relationships between animals and pastures. Therefore, this review aims to describe some aspects of the feeding behavior profiles of both sheep and goats in grazing systems. The structure of the pasture is a key factor in the feeding behavior of grazing animals. The amount of feed consumed in a given period of time is affected by the number of meals, duration and velocity of swallowing, changes in grazing time, bite rate, bite weight, and quality of ingested forage. The different phenological stages of forage also influence the animals’ strategies to optimize their intake, which consequently changes their behavioral activities. Sheep and goats tend to be more selective than cattle, and young animals are more selective than older animals; this selectivity characteristicis one of the most important aspects to be observed in pasture management. According to the degree of selectivity, the animals will intake forages of higher or lower nutritive quality. In addition, the intensity and distribution of their daily activities (grazing, ruminating, and resting)are influenced by several factors, such as the availability and nutritive value of the pasture, its management, the animal activity in the group, and the predominant climatic conditions of the region.


Subject(s)
Animals , Feeding Behavior , Sheep/anatomy & histology , Sheep/growth & development , Pasture/analysis , Ruminants/anatomy & histology , Ruminants/growth & development
8.
Rev. bras. reprod. anim ; 45(4): 361-368, out.-dez. 2021. graf
Article in English | VETINDEX | ID: biblio-1492682

ABSTRACT

La estacionalidad de la reproducción es una característica de la mayoría de las razas caprinas y ovinas de latitudes subtropicales. Las hembras presentan anovulación y disminución de las concentraciones plasmáticas de LH en primavera y verano, mientras que los machos disminuyen drásticamente los niveles plasmáticos de testosterona, la producción espermática y el comportamiento sexual en invierno y primavera. Esta estacionalidad es sincronizada principalmente por las variaciones naturales del fotoperiodo. Sin embargo, las interacciones socio-sexuales entre machos y hembras permiten modificar esta estacionalidad. Así, la introducción de machos en grupos de hembras en anestro estacional estimulan las actividades endocrina y reproductiva de las hembras, através del fonómeno conocido como "efecto macho". La respuesta de las hembras al efecto macho varía con la intensidad del comportamiento sexual desplegado por los machos. Por ello, los machos sexualmente activos son más eficaces que los machos en reposo sexual para estimula la ovulación de las hembras. Además, la presencia continua de los machos sexualmente activos evita la anovulación estacional, así como la disminución estacional de las concentraciones plasmáticas de LH en hembras ovariectomizadas portadoras de implantes subcutáneos que liberan estradiol. En conjunto, estos hallazgos indican que los machos cabríos y carneros sexualmente activos son una alternativa para manipular la estacionalidad reproductiva de las hembras durante el anestro estacional, y/o evitar la anovulación estacional. Además, considerando las demandas sociales para reducir o eliminar los tratamientos hormonales en la producción animal, la asociación del fotoperiodo y las interacciones socio-sexuales entre machos y hembras constituye una alternativa sustentable para la manipulación de la actividad reproductiva de los pequeños rumiantes.


Subject(s)
Animals , Luteinizing Hormone/analysis , Luteinizing Hormone/chemistry , Ovulation , Ruminants/anatomy & histology , Seasons , Photoperiod , Sheep , Ruminants
9.
B. Indústr. Anim. ; 77: 1-10, 11 ago. 2020. tab
Article in Portuguese | VETINDEX | ID: vti-27586

ABSTRACT

Objetivou-se avaliar a inclusão de ureia na dieta de cabritos sobre o desempenho e características de carcaça. Foram utilizados 18 cabritos (150 dias de idade e 21 Kg de peso) distribuídos em dois grupos com dietas isoproteicas. Os animais do grupo controle (GC) foram alimentados com ração sem ureia na sua composição, enquanto os animais do outro grupo (GU) foram alimentados com ração com 1% da MS de ureia na base da matéria seca. Avaliou-se o ganho de peso médio (g/dia), biometrias corporais, assim como o peso e rendimento de carcaça nos animais. Não houve diferenças significativas em nenhuma das variáveis estudadas (p>0,05), como: ganho de peso (g/dia) (GU: 122,1 ± 11,89 vsGC: 132,9 ± 4,97), perímetro torácico (cm) (GU: 73,0 ± 1,2 vsGC: 74,5 ± 0,57), comprimento corporal (cm) (GU: 74,0 ± 1,42 vsGC: 73,67 ± 0,70), peso ao abate (kg) (GU: 34,39 ± 1,76 vsGC: 36,56 ± 0,96), rendimentos de carcaça quente (%) (GU: 47,37 ± 0,82 vsGC: 47,23 ± 0,72) e fria (GU: 45,64 ± 0,60 vsGC: 45,84 ± 0,70). Conclui-se que o uso de 1% de ureia na MS da dieta de caprinos machos jovem não afeta o desempenho dos animais e as características de carcaça.(AU)


The objective of this study was to evaluate the effect of inclusion of urea in the diet of goats on performance and carcass traits. Eighteen kids (150 days old and 21 kg of weight) were divided into two groups receiving isoprotein diets. The animals in the control group (CG) were fed ration without urea, while the animals in the urea group (UG) were fed ration with 1% urea on a dry matter basis. Average weight gain (g/day), body biometrics, and carcass weight and yield were evaluated in the animals. There were no significant differences in any of the variables studied (p>0.05): weight gain (g/day) (UG: 122.1 ± 11.89 vsCG: 132.9 ± 4.97), chest circumference (cm) (UG: 73.0 ± 1.2 vsCG: 74.5 ± 0.57), body length (cm) (UG: 74.0 ± 1.42 vsCG: 73.67 ± 0.70), slaughter weight (kg) (UG: 34.39 ± 1.76 vsCG: 36.56 ± 0.96), hot carcass yield (%) (UG: 47.37 ± 0.82 vsCG: 47.23 ± 0.72), or cold carcass yield (UG: 45.64 ± 0.60 vsCG: 45.84 ± 0.70). In conclusion, the addition of 1% urea in the DM to the diet of young male goats does not affect the performance of the animals or carcass traits.(AU)


Subject(s)
Animals , Ruminants/anatomy & histology , Urea/administration & dosage , Animal Feed , Body Weights and Measures , Weight Gain , Nitrogen
10.
Bol. ind. anim. (Impr.) ; 77: 1-10, 7 fev. 2020. tab
Article in Portuguese | VETINDEX | ID: biblio-1467000

ABSTRACT

Objetivou-se avaliar a inclusão de ureia na dieta de cabritos sobre o desempenho e características de carcaça. Foram utilizados 18 cabritos (150 dias de idade e 21 Kg de peso) distribuídos em dois grupos com dietas isoproteicas. Os animais do grupo controle (GC) foram alimentados com ração sem ureia na sua composição, enquanto os animais do outro grupo (GU) foram alimentados com ração com 1% da MS de ureia na base da matéria seca. Avaliou-se o ganho de peso médio (g/dia), biometrias corporais, assim como o peso e rendimento de carcaça nos animais. Não houve diferenças significativas em nenhuma das variáveis estudadas (p>0,05), como: ganho de peso (g/dia) (GU: 122,1 ± 11,89 vsGC: 132,9 ± 4,97), perímetro torácico (cm) (GU: 73,0 ± 1,2 vsGC: 74,5 ± 0,57), comprimento corporal (cm) (GU: 74,0 ± 1,42 vsGC: 73,67 ± 0,70), peso ao abate (kg) (GU: 34,39 ± 1,76 vsGC: 36,56 ± 0,96), rendimentos de carcaça quente (%) (GU: 47,37 ± 0,82 vsGC: 47,23 ± 0,72) e fria (GU: 45,64 ± 0,60 vsGC: 45,84 ± 0,70). Conclui-se que o uso de 1% de ureia na MS da dieta de caprinos machos jovem não afeta o desempenho dos animais e as características de carcaça.


The objective of this study was to evaluate the effect of inclusion of urea in the diet of goats on performance and carcass traits. Eighteen kids (150 days old and 21 kg of weight) were divided into two groups receiving isoprotein diets. The animals in the control group (CG) were fed ration without urea, while the animals in the urea group (UG) were fed ration with 1% urea on a dry matter basis. Average weight gain (g/day), body biometrics, and carcass weight and yield were evaluated in the animals. There were no significant differences in any of the variables studied (p>0.05): weight gain (g/day) (UG: 122.1 ± 11.89 vsCG: 132.9 ± 4.97), chest circumference (cm) (UG: 73.0 ± 1.2 vsCG: 74.5 ± 0.57), body length (cm) (UG: 74.0 ± 1.42 vsCG: 73.67 ± 0.70), slaughter weight (kg) (UG: 34.39 ± 1.76 vsCG: 36.56 ± 0.96), hot carcass yield (%) (UG: 47.37 ± 0.82 vsCG: 47.23 ± 0.72), or cold carcass yield (UG: 45.64 ± 0.60 vsCG: 45.84 ± 0.70). In conclusion, the addition of 1% urea in the DM to the diet of young male goats does not affect the performance of the animals or carcass traits.


Subject(s)
Animals , Weight Gain , Nitrogen , Body Weights and Measures , Animal Feed , Ruminants/anatomy & histology , Urea/administration & dosage
11.
Anim. Reprod. ; 17(2): e20190115, 2020. ilus, tab
Article in English | VETINDEX | ID: vti-29308

ABSTRACT

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.(AU)


Subject(s)
Animals , Male , Female , Mice , Ruminants/anatomy & histology , Ruminants/physiology , Vitrification , Follicular Phase , Cryopreservation , Transplantation, Heterologous
12.
Anim. Reprod. ; 17(3): [e20200069], 2020. ilus, tab, graf
Article in English | VETINDEX | ID: vti-27859

ABSTRACT

Reproductive efficiency is critically dependent on embryo survival, establishment of a successful pregnancy and placental development. Recent advances in gene editing technology have enabled investigators to use gene knockdown and knockout approaches to better understand the role of hormone signaling in placental function and fetal growth and development. In this review, an overview of ruminant placentation will be provided, including recent data highlighting the role of histone lysine demethylase 1A and androgen signaling in ruminant placenta and pregnancy. Studies in ruminant placenta establish a role for histone lysine demethylase 1A in controlling genetic networks necessary for important cellular events such as cell proliferation and angiogenesis, as well as androgen receptor signaling during early placentation.(AU)


Subject(s)
Animals , Female , Ruminants/anatomy & histology , Ruminants/genetics , Ruminants/physiology , Placenta , Histone Demethylases , Androgens
13.
Acta Vet. bras. ; 14(2): 121-131, jun. 2020. mapas, tab
Article in English | VETINDEX | ID: vti-27009

ABSTRACT

Characterization of the profile of goat and sheep production in Sergipe enables better understanding of the main barriers linked to low productivity, generating important information for the development of this sector. The objective was to identify and characterize the production of goats and sheep in Sergipe. From 2011 to 2014, 41 goat properties and 60 sheep properties were analyzed, located in 25 municipalities in the three mesoregions of the State (Leste, Agreste, and Sertão). The information was obtained through application of a structured questionnaire to the farmers addressing topics such as: characterization of the property, herd, facilities, sanitary, nutritional, and reproductive management, and socioeconomic aspects of the farmer.  The results demonstrated that 58.5% (24/41) of goat farms had an area less than 30 hectares (ha), reared predominantly mixed breed animals (SRD), with low use of technologies, aiming at family consumption, and complementing income through local marketing. On the sheep properties, 58.3% (35/60) were over 100 ha and used for the production of Santa Inês sheep and crossbreeds together with cattle. Significant flaws were also observed in feed, sanitary, and reproductive management, emphasizing the non-use of preserved foods and forages typical of the region, factors that contribute to the low level of productivity and ustainability of production in a viable and homogeneous manner. The findings led to the conclusion that more organized production is necessary, mainly through investment in technologies and qualified and continuous rural extension, aiming at improving the production, productivity, income, and quality of life of the breeders.(AU)


A caracterização do perfil das criações possibilita um maior entendimento dos principais entraves ligados a baixa produtividade e auxilia na disponibilização de dados importantes para a busca de soluções para crescimento da ovinocaprinocultura. O presente trabalho teve como objetivo caracterizar o sistema de produção de caprinos e ovinos no estado de Sergipe. Foram visitadas entre os anos de 2011 e 2014, 41 e 60 propriedades de caprinos e ovinos, respectivamente, localizadas em 25 municípios distribuídos nas três mesorregiões do Estado (Leste, Agreste e Sertão). O levantamento dos dados ocorreu pela aplicação de questionário junto aos proprietários, com temas referentes às características do rebanho, instalações, manejo sanitário, nutricional e reprodutivo eperfil do criador. A caprinocultura foi caracterizada como atividade complementar da renda de pequenos produtores rurais, com a maioria do rebanho representado por animais de padrão racial não definido e pela raça Saanen. A atividade é voltada ao consumo familiar com baixo uso tecnológico e produção leiteira para atendimento do comércio local. Enquanto que na ovinocultura, os rebanhos são compostos por animais puros e mestiços da raça Santa Inês mantidos em grandes áreas de terra devido a sua criação consorciada com bovinos. As falhas de manejo observadas nos rebanhos caprinos e ovinos comprometem a viabilidade do sistema de produção, contribuindo com maiores desafios para ovinocaprinocultura sergipana em atender a demanda do mercado consumidor do Estado(AU)


Subject(s)
Animals , Ruminants/anatomy & histology , Sheep/anatomy & histology , Consumer Behavior , Veterinary Medicine
14.
Acta sci., Anim. sci ; 42: e48225, out. 2020. tab
Article in English | VETINDEX | ID: vti-26703

ABSTRACT

The objective of this study was to evaluate non-carcass components of goats submitted to grazing in the Caatinga rangeland and supplemented with spineless cactus (Nopalea cochelinifera Salm Dick), Jitirana hay (Merremia aegyptia L. Urban) and Mororó hay (Bauhinia cheilanta Bong Stend). Thirty male goats (castrated), with no defined breed, with an initial mean body weight of 19 ± 0.35 kg and approximately 90 days of age were used. The treatments consisted of grazing without supplementation (GWS), grazing + Jitirana hay (GJ); grazing + Jitirana hay + spineless cactus (GJSC); grazing + Mororó hay (GM); grazing + Mororó hay + spineless cactus (GMSC). The means of the variables were tested by Tukey's test at 5% probability. The goats fed GWS, GJSC, GM and GMSC presented higher weights and yields of the diaphragm, and the spleen weight (p < 0.05). The highest yield of omasum (p < 0.05) occurred with goats fed GJ treatment. There were treatments (p < 0.05) on omental fat weight and leg yield, with the highest values for goats, fed GWS, GJSC, GM, and GMSC. The "Buchada" EBW-1 yield was higher (p < 0.05) for animals fed GWS. Feeding supplementation of goats grazing in the Caatinga had few influences on weights and yields of non-carcass componentes.(AU)


Subject(s)
Animals , Cactaceae/growth & development , Ruminants/anatomy & histology , Ruminants/metabolism , Pasture/analysis , Infant Nutritional Physiological Phenomena
15.
Acta sci., Anim. sci ; 42: e48225, out. 2020. tab
Article in English | VETINDEX | ID: biblio-1459909

ABSTRACT

The objective of this study was to evaluate non-carcass components of goats submitted to grazing in the Caatinga rangeland and supplemented with spineless cactus (Nopalea cochelinifera Salm Dick), Jitirana hay (Merremia aegyptia L. Urban) and Mororó hay (Bauhinia cheilanta Bong Stend). Thirty male goats (castrated), with no defined breed, with an initial mean body weight of 19 ± 0.35 kg and approximately 90 days of age were used. The treatments consisted of grazing without supplementation (GWS), grazing + Jitirana hay (GJ); grazing + Jitirana hay + spineless cactus (GJSC); grazing + Mororó hay (GM); grazing + Mororó hay + spineless cactus (GMSC). The means of the variables were tested by Tukey's test at 5% probability. The goats fed GWS, GJSC, GM and GMSC presented higher weights and yields of the diaphragm, and the spleen weight (p < 0.05). The highest yield of omasum (p < 0.05) occurred with goats fed GJ treatment. There were treatments (p < 0.05) on omental fat weight and leg yield, with the highest values for goats, fed GWS, GJSC, GM, and GMSC. The "Buchada" EBW-1 yield was higher (p < 0.05) for animals fed GWS. Feeding supplementation of goats grazing in the Caatinga had few influences on weights and yields of non-carcass componentes.


Subject(s)
Animals , Cactaceae/growth & development , Ruminants/anatomy & histology , Ruminants/metabolism , Infant Nutritional Physiological Phenomena , Pasture/analysis
16.
Acta Vet. Brasilica ; 14(2): 121-131, jun. 2020. map, tab
Article in English | VETINDEX | ID: biblio-1453216

ABSTRACT

Characterization of the profile of goat and sheep production in Sergipe enables better understanding of the main barriers linked to low productivity, generating important information for the development of this sector. The objective was to identify and characterize the production of goats and sheep in Sergipe. From 2011 to 2014, 41 goat properties and 60 sheep properties were analyzed, located in 25 municipalities in the three mesoregions of the State (Leste, Agreste, and Sertão). The information was obtained through application of a structured questionnaire to the farmers addressing topics such as: characterization of the property, herd, facilities, sanitary, nutritional, and reproductive management, and socioeconomic aspects of the farmer.  The results demonstrated that 58.5% (24/41) of goat farms had an area less than 30 hectares (ha), reared predominantly mixed breed animals (SRD), with low use of technologies, aiming at family consumption, and complementing income through local marketing. On the sheep properties, 58.3% (35/60) were over 100 ha and used for the production of Santa Inês sheep and crossbreeds together with cattle. Significant flaws were also observed in feed, sanitary, and reproductive management, emphasizing the non-use of preserved foods and forages typical of the region, factors that contribute to the low level of productivity and ustainability of production in a viable and homogeneous manner. The findings led to the conclusion that more organized production is necessary, mainly through investment in technologies and qualified and continuous rural extension, aiming at improving the production, productivity, income, and quality of life of the breeders.


A caracterização do perfil das criações possibilita um maior entendimento dos principais entraves ligados a baixa produtividade e auxilia na disponibilização de dados importantes para a busca de soluções para crescimento da ovinocaprinocultura. O presente trabalho teve como objetivo caracterizar o sistema de produção de caprinos e ovinos no estado de Sergipe. Foram visitadas entre os anos de 2011 e 2014, 41 e 60 propriedades de caprinos e ovinos, respectivamente, localizadas em 25 municípios distribuídos nas três mesorregiões do Estado (Leste, Agreste e Sertão). O levantamento dos dados ocorreu pela aplicação de questionário junto aos proprietários, com temas referentes às características do rebanho, instalações, manejo sanitário, nutricional e reprodutivo eperfil do criador. A caprinocultura foi caracterizada como atividade complementar da renda de pequenos produtores rurais, com a maioria do rebanho representado por animais de padrão racial não definido e pela raça Saanen. A atividade é voltada ao consumo familiar com baixo uso tecnológico e produção leiteira para atendimento do comércio local. Enquanto que na ovinocultura, os rebanhos são compostos por animais puros e mestiços da raça Santa Inês mantidos em grandes áreas de terra devido a sua criação consorciada com bovinos. As falhas de manejo observadas nos rebanhos caprinos e ovinos comprometem a viabilidade do sistema de produção, contribuindo com maiores desafios para ovinocaprinocultura sergipana em atender a demanda do mercado consumidor do Estado


Subject(s)
Animals , Consumer Behavior , Sheep/anatomy & histology , Ruminants/anatomy & histology , Veterinary Medicine
17.
Anim. Reprod. (Online) ; 17(2): e20190115, 2020. ilus, tab
Article in English | VETINDEX | ID: biblio-1461504

ABSTRACT

Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.


Subject(s)
Male , Female , Animals , Mice , Cryopreservation , Follicular Phase , Ruminants/anatomy & histology , Ruminants/physiology , Vitrification , Transplantation, Heterologous
18.
Anim. Reprod. (Online) ; 17(3): [e20200069], 2020. ilus, tab, graf
Article in English | VETINDEX | ID: biblio-1461517

ABSTRACT

Reproductive efficiency is critically dependent on embryo survival, establishment of a successful pregnancy and placental development. Recent advances in gene editing technology have enabled investigators to use gene knockdown and knockout approaches to better understand the role of hormone signaling in placental function and fetal growth and development. In this review, an overview of ruminant placentation will be provided, including recent data highlighting the role of histone lysine demethylase 1A and androgen signaling in ruminant placenta and pregnancy. Studies in ruminant placenta establish a role for histone lysine demethylase 1A in controlling genetic networks necessary for important cellular events such as cell proliferation and angiogenesis, as well as androgen receptor signaling during early placentation.


Subject(s)
Female , Animals , Histone Demethylases , Placenta , Ruminants/anatomy & histology , Ruminants/physiology , Ruminants/genetics , Androgens
19.
Arq. bras. med. vet. zootec. (Online) ; 71(1): 340-344, jan.-fev. 2019. tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1038588

ABSTRACT

The morphology of the ruminal and omasal mucosa and hepatic parenchyma of 44 young bulls submitted to four types of concentrates were evaluated by varying the inclusion level of crude Glycerin - CG (0, 6, 12 and 18%). The variables evaluated were number of ruminal papillae per cm² of wall; area of the papillae and total absorption surface per cm² of wall; Mitotic index - MI, height and area of ruminal and omasal papillae; and hepatic morphology. The samples processed for inclusion in paraffin and stained with Hematoxylin-Eosin. The different levels of inclusion of CG in the diet did not interfere either in the number of papillae per cm2 of rumen wall (P= 0,70) or in the ruminal absorptive surface area (P= 0,89). Animals that received diets with levels 12 and 18% had higher MI of the ruminal epithelium (P< 0.01), but higher cell proliferation wasn't reflected in height gain and papillary area (P= 0,82). The omasal mucosa wasn't influenced by the inclusion of CG (MI e height gain and papillary area, P= 0,43, P= 0,56 e P= 0,9, respectively). The inclusion of GB in up to 18% of diet dry matter didn't alter the morphology of the rumen and omaso mucosa; and the integrity hepatic parenchyma.(AU)


Subject(s)
Animals , Cattle , Omasum/anatomy & histology , Rumen/anatomy & histology , Ruminants/anatomy & histology , Glycerol/administration & dosage , Liver/anatomy & histology
20.
Pesqui. vet. bras ; 38(3): 444-449, mar. 2018. tab, ilus
Article in English | VETINDEX | ID: vti-19384

ABSTRACT

Neospora caninum has been described as a parasite that sporadically causes reproductive problems in goats. Several aspects of the pathogenesis of neosporosis in naturally infected goats remain to be established. The aims of the present study were to characterize the placental lesions in goats naturally infected by N. caninum and to evaluate several diagnostic techniques for effective detection of this protozoan in the goat placenta. Some placentas in this study originated from abortion and stillbirth in which there were severe lesions. The lesions were characterized mainly by necrosis involving the mesenchyme of the chorionic villi and trophoblast cells often alongside mononuclear inflammation and in some cases with neutrophilic infiltration. N. caninum DNA was detected in these placentas, but parasite structures were not visualized through immunohistochemistry (IHC). However, five of 11 placentas from N. caninum-infected goats that gave birth to healthy kids had histological lesions characterized by mononuclear inflammation. Of these 11 placentas, N. caninum DNA was detected in seven, and N. caninum tachyzoites were detected in only one of these seven placentas using IHC. The present study demonstrates that severe lesions in the placenta are associated with abortion and stillbirth in caprine neosporosis and the placental alterations are likely involved in abortion pathogenesis. Moreover, the results highlight the importance of using more than one diagnostic technique for the detection of the protozoan in placentas because N. caninum cannot be reliably detected by histological and immunohistochemical tests.(AU)


Neospora caninum é descrito como um parasito que causa problemas reprodutivos esporádicos em cabras. Muitos aspectos da patogênese da neosporose em cabras naturalmente infectadas ainda precisam ser estabelecidos. Os objetivos deste trabalho foram caracterizar as lesões placentárias em cabras naturalmente infectadas por N. caninum e avaliar as técnicas diagnósticas para a detecção efetiva do protozoário na placenta. Algumas placentas deste estudo são originárias de abortos e natimortos, nas quais havia lesões graves. As lesões foram classificadas principalmente por necrose envolvendo o mesênquima das vilosidades coriônicas e células trofoblásticas, geralmente associadas a infiltrado inflamatório mononuclear e em alguns casos infiltrado neutrofílico. O DNA do N. caninum foi detectado nestas placentas, porém estruturas parasitárias não foram visualizadas na imuno-histoquímica (IHQ). Entretanto, cinco das onze placentas de cabras infectadas, que deram à luz a conceptos saudáveis, apresentaram lesões histológicas caracterizadas por infiltrado inflamatório mononuclear. Destas 11 placentas, foi detectado DNA de N. caninum em sete e taquizoítos foram encontrados em apenas uma por meio de imuno-histoquímica. O presente estudo demonstra que abortos e natimortos na espécie caprina, causados pelo N. caninum estão associados a lesões acentuadas nas placentas, sendo que as mesmas estão envolvidas na patogênese do aborto. Os resultados também ressaltam a importância do uso de mais de uma técnica diagnóstica para a detecção do protozoário em placentas, pois o N. caninum não pode ser confiavelmente detectado somente pelos exames de histopatologia e imuno-histoquímica.(AU)


Subject(s)
Animals , Female , Ruminants/anatomy & histology , Neospora/parasitology , Abortion, Veterinary/diagnosis , Placenta Diseases/veterinary
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