ABSTRACT
Glycation starts from nonenzymatic amino-carbonyl reaction that binds carbonyl group of reducing sugars to the amino group of amino acids. Glycation leads to further complex reactions to form advanced glycation end products (AGE). Because AGE are implicated in the gradual development of diabetic complications, tissue accumulation of AGE has been widely examined in various tissues of rats. Avian species are known to have high body temperature and blood glucose concentration compared with mammals. Although these characteristics enabled chickens to be used as experimental models for diabetes mellitus, the information of AGE accumulation in various tissues of chickens has not been limited so far. In the present study, therefore, the radioactive AGE prepared by reacting (14)C-glucose and amino acids were intravenously administrated, and comparison of tissue accumulation of (14)C-labeled AGE was made between chickens and rats. At 30 min after administration, tissues (18-20) were collected, and the radioactivity incorporated into tissues was determined. High levels of radioactivity per gram of tissue in the liver and kidney were observed in both rats and chickens. In chickens but not rats, a large amount of (14)C-labeled AGE incorporated into 1 g of spleen was observed, and the specific accumulation of AGE in the avian spleen might have a particular role in immune response in avian species.
Subject(s)
Amino Acids/metabolism , Chickens/metabolism , Glucose/metabolism , Glycation End Products, Advanced/metabolism , Rats/metabolism , Spleen/metabolism , Administration, Intravenous/veterinary , Amino Acids/administration & dosage , Animals , Carbon Radioisotopes , Chickens/growth & development , Glucose/administration & dosage , Male , Organ Specificity , Rats/growth & development , Rats, Wistar/metabolism , Scintillation Counting/veterinaryABSTRACT
OBJECTIVE: The aim of this work was to evaluate a pixelated semiconductor detector and non-pixelated scintillation detector in a pinhole SPECT system for small animal imaging. METHODS: We assumed two pixelated CdTe semiconductor detectors (a monolithic type and a modular type) and two non-pixelated NaI(Tl) scintillation detectors (a conventional type and a large detector field type). For the monolithic semiconductor detector we assumed that the size of a pixel was 1.0 × 1.0 mm², the thickness 1 mm, and an effective detector field 128 × 128 mm². For the modular-type semiconductor detector we assumed that the size of a pixel was 2.5 × 2.5 mm², the thickness 5 mm, and an effective detector field 320 × 320 mm². For the two scintillation detectors we assumed that the size of a pixel was 1.4 × 1.4 mm² and the intrinsic spatial resolution 4.0 mm FWHM, and the thickness 9 mm. For the conventional scintillation detector we assumed that the effective detector field was 179.2 × 179.2 mm², and for the large field scintillation detector 358.2 × 358.2 mm² and the magnification factor two. In the simulation we used a pinhole collimator with a pinhole size of 0.3 mm. We reconstructed SPECT images of hot-rod and cold-channel phantoms with projection data calculated with a Monte Carlo method assuming a fixed data acquisition time, and evaluated the image quality with respect to contrast and spatial resolution. In addition, we calculated the scatter fraction to compare the amount of scattered photons between the pixelated and non-pixelated detectors. RESULTS: The image quality of the modular-type pixelated detector was similar to that of the non-pixelated detector operated with a twofold magnified data acquisition. The scattered photons and the parallax effect in the pixelated detector were small and similar to those in the non-pixelated detector. CONCLUSIONS: The performance of a modular-type pixelated semiconductor detector was almost the same as that of a non-pixelated scintillation detector with a magnified data acquisition in a small animal pinhole SPECT system.
Subject(s)
Body Size , Scintillation Counting/veterinary , Semiconductors/veterinary , Tomography, Emission-Computed, Single-Photon/veterinary , Animals , Image Processing, Computer-Assisted , Monte Carlo Method , Scintillation Counting/instrumentation , Semiconductors/instrumentation , Tomography, Emission-Computed, Single-Photon/instrumentationABSTRACT
Although radioactive iodide uptake (RAIU) is one of the reliable diagnostic methods for thyroid function in adult humans, especially in the diagnosis of thyrotoxicosis, there are limited data for RAIU during pregnancy and lactation in humans and animals. Therefore, we proposed to validate RAIU for the lactating rhesus monkey to further human model studies in thyroid disease. RAIU was performed at 6 and 24 h using 100 microCi of (123)I orally in four lactating monkeys. The thyroid and thigh were counted using a scintillation probe and multichannel analyser. A dose/standard ratio of counts/minute was calculated to compensate for background, utilizing differences in the activity between the dose administered and a standard. Thyroidal RAIU varied significantly among monkeys: 6.71 +/- 2.40% for the 6 h uptake and 15.44 +/- 7.71% for the 24 h uptake. These data showed that the RAIU test may allow a rational clinical approach to thyroid function testing for lactating rhesus monkeys. Additional studies are needed for assessing thyroid function in rhesus monkeys of varying ages and gender with clinical abnormalities.
Subject(s)
Iodine Radioisotopes/pharmacokinetics , Macaca mulatta/metabolism , Thyroid Function Tests/methods , Thyroid Function Tests/veterinary , Thyroid Gland/metabolism , Animals , Female , Lactation , Pregnancy , Scintillation Counting/veterinaryABSTRACT
OBJECTIVE: To evaluate the effect of infection with bovine respiratory syncytial virus (BRSV) on clearance of inhaled antigens from the lungs of calves. ANIMALS: Eleven 6- to 8-week-old Holstein bull calves. PROCEDURES: Aerosolized (99m)technetium ((99m)Tc)-labeled diethylene triamine pentacetate (DTPA; 3 calves), commonly used to measure integrity of the pulmonary epithelium, and (99m)Tc-labeled ovalbumin (OA; 8 calves), commonly used as a prototype allergen, were used to evaluate pulmonary clearance before, during, and after experimentally induced infection with BRSV or sham inoculation with BRSV. Uptake in plasma (6 calves) and lung-efferent lymph (1 calf) was examined. RESULTS: Clearance of (99m)Tc-DTPA was significantly increased during BRSV infection; clearance of (99m)Tc-OA was decreased on day 7 after inoculation. Clearance time was correlated with severity of clinical disease, and amounts of (99m)Tc-OA in plasma and lymph were inversely correlated with clearance time. Minimum amounts of (99m)Tc-OA were detected at time points when pulmonary clearance of (99m)Tc-OA was most delayed. CONCLUSIONS AND CLINICAL RELEVANCE: BRSV caused infection of the respiratory tract with peak signs of clinical disease at 7 or 8 days after inoculation. Concurrently, there was a diminished ability to move inhaled protein antigen out of the lungs. Prolonged exposure to inhaled antigens during BRSV infection may enhance antigen presentation with consequent allergic sensitization and development of chronic inflammatory lung disease. IMPACT FOR HUMAN MEDICINE: Infection of humans with respiratory syncytial virus early after birth is associated with subsequent development of allergic asthma. Results for BRSV infection in these calves suggested a supportive mechanism for this scenario.
Subject(s)
Allergens/pharmacokinetics , Cattle Diseases/metabolism , Cattle Diseases/virology , Respiratory Syncytial Virus Infections/veterinary , Respiratory Syncytial Virus, Bovine/immunology , Respiratory Tract Diseases/veterinary , Animals , Antibodies, Viral/blood , Blood Gas Analysis/veterinary , Cattle , Cattle Diseases/immunology , Male , Respiratory Syncytial Virus Infections/immunology , Respiratory Syncytial Virus Infections/metabolism , Respiratory Syncytial Virus Infections/virology , Respiratory Tract Diseases/immunology , Respiratory Tract Diseases/metabolism , Respiratory Tract Diseases/virology , Scintillation Counting/veterinary , Technetium Tc 99m Pentetate/blood , Technetium Tc 99m Pentetate/pharmacokinetics , Virus Shedding/immunologyABSTRACT
The present study aimed at analysis of the effects of polyunsaturated fatty acid (PUFA), linoleic acid (LA, C18:2n - 6) and linolenic acid (LNA, C18:3n - 3) on bovine peripheral blood mononuclear cells (PBMC) in vitro. Both mitogen (ConA)-induced proliferative lymphocyte responsiveness during 4 days of culture and eicosanoid (prostaglandin E(2) (PGE(2)) and leukotriene B(4) (LTB(4))) production during 36 h were determined in relation to the absence or presence of various concentrations of LA and LNA (0, 1, 5, 25, 125 and 250 microM). Mitogen-driven proliferative responses of lymphocytes tended to be uninfluenced in the presence of lower concentrations of LA, whereas significant inhibition was observed at the higher concentrations of LA (125 and 250 microM). However, increasing amounts of LNA did not affect the proliferation. ConA stimulation induced a clear PGE(2) response, which significantly decreased in the presence of 250 microM of LA. In addition, increasing amounts of LNA, but not LA, led to a significant decrease in LTB(4) levels. However, The production of LTB(4) did not alter due to mitogenic stimulation. In conclusion, the present study shows that bovine mononuclear cells may functionally be influenced by the presence of PUFA in their environment. Further studies need to be conducted to clarify in vivo consequences of these findings in a situation of PUFA enriched rations in ruminants.
Subject(s)
Leukocytes, Mononuclear/drug effects , Linoleic Acid/pharmacology , Lymphocyte Activation/drug effects , alpha-Linolenic Acid/pharmacology , Animals , Cattle , Concanavalin A/immunology , Dinoprostone/analysis , Dinoprostone/immunology , Female , Immunoenzyme Techniques/veterinary , Leukocytes, Mononuclear/cytology , Leukocytes, Mononuclear/immunology , Leukotriene B4/analysis , Leukotriene B4/immunology , Linoleic Acid/immunology , Lymphocyte Activation/immunology , Scintillation Counting/veterinary , Thymidine/metabolism , alpha-Linolenic Acid/immunologyABSTRACT
Interferon (INF) activity was evaluated in the supernatants from peripheral white blood cells (WBC) of chickens from six lines. The WBC were cultured in flasks or 24-well plates with medium or medium and phytohemagglutinin (PHA). After 2 to 5 d, duplicate supernatant samples were tested for INF activity, i.e., the log2 titer inhibiting 50% destruction of the cytopathic effect of vesicular stomatitis virus on primary chick embryo fibroblasts. Also triplicate WBC samples were tested for proliferation by [H3]-thymidine labeling and scintillation counting. In the absence of PHA, INF was significant for only two lines, i.e., 7(2) (two trials) and C (one trial). With PHA the level of INF produced was similar if flasks were sampled daily or on successive days. The INF levels were highest using 10 or 20 microg/mL PHA, but line differences were best distinguished using 5 or 10 microg/mL. In three trials there was a low correlation between PHA-stimulated WBC proliferation and INF titer (r > or = 0.30; P < 0.05). It was concluded that supernatants from chicken WBC stimulated with 10 microg/mL PHA contain INF, and inbred Lines 72 and C repeatedly produce more INF than inbred Lines 63 and 15I(5). This is the first evidence for line differences in INF production in chickens, and these lines may be useful for characterization of the relevant genes and their importance in immune response(s) and disease resistance.
Subject(s)
Chickens/immunology , Interferons/biosynthesis , Leukocytes/immunology , Lymphocyte Activation/drug effects , Phytohemagglutinins/pharmacology , Animals , Cells, Cultured , Chick Embryo , Chickens/blood , Chickens/genetics , Leukocyte Count/veterinary , Leukocytes/drug effects , Random Allocation , Scintillation Counting/veterinaryABSTRACT
14C-labeled flumequine was administered as a single oral (5 mg kg(-1), 86 microCi kg(-1)) or intravenous (5 mg kg(-1), 82 microCi kg(-1)) dose to Atlantic salmon Salmo salar held in sea water or in fresh water. The absorption, tissue distribution and elimination were determined by means of liquid scintillation counting and whole-body autoradiography. The drug was rapidly absorbed and extensively distributed in all groups of fish. Radiolabeled compound was present in blood and muscle for more than 8 wk in the freshwater groups. In the seawater groups, however, no radioactivity was detected in the blood and muscle after 4 d and 2 wk, respectively. It was concluded that flumequine was eliminated at a substantially higher rate from Atlantic salmon in sea water than in fresh water.
Subject(s)
Anti-Infective Agents/pharmacokinetics , Fluoroquinolones , Fresh Water , Quinolizines/pharmacokinetics , Salmo salar/metabolism , Seawater , Absorption , Administration, Oral , Animals , Anti-Infective Agents/administration & dosage , Autoradiography/veterinary , Biological Availability , Injections, Intravenous/veterinary , Quinolizines/administration & dosage , Scintillation Counting/veterinary , Tissue DistributionABSTRACT
The aim of this study was to investigate the effect of gonadotrophin-releasing hormone (GnRH) immunisation on mature stallions that had been used for breeding. Four Standardbred stallions were used in the study: 3 experimental animals and 1 control animal. Semen was collected regularly, i.e. twice/week, during the 4 months prior to the experimental period. The stallions were immunised against GnRH with a GnRH-BSA conjugate. Equimune was used as the adjuvant. The stallions were immunised on 5 occasions, 4 at 2 week intervals, and the fifth 4 weeks after the fourth. Blood samples were taken once a week for analysis of GnRH antibody titre and every third week for testosterone and oestrone sulphate analyses. Semen was collected once a week, and libido and sexual behaviour were observed. Ejaculate volume, sperm concentration, total number of sperm in the ejaculate, sperm motility and sperm morphology were evaluated. Testicular size was measured once a week. At the end of the study, the stallions were castrated, and a histological examination of the testes performed. All immunised stallions produced antibodies against GnRH, and plasma testosterone concentration decreased. However, the effect of immunisation varied between stallions. In 2 of the stallions, high levels of antibodies were found, while in the third, the level was moderate. Four weeks after the first immunisation, a decrease in libido was observed. Two months after the first immunisation, marked changes in semen quality were observed in the 2 stallions with high antibody titres. Fourteen weeks after the first immunisation, the total number of sperm/ejaculate had decreased from >8.6 x 10(9) to <2.7 x 10(9), sperm motility from >59 to <10% and the frequency of morphological normal spermatozoa had decreased from >60 to <14%. The dominating abnormalities were abnormal head shapes, proximal cytoplasmic droplets and detached heads. In the third stallion, only slight changes in semen quality were found. No changes were observed in the control stallion. Decreases in testicular size were noted in all of the experimental stallions. Pronounced histological alterations in the testes were observed in 2 of the stallions. It is concluded that the vaccine was effective in stimulating production of GnRH antibodies and in suppressing testicular function and androgen secretion. However, there was an individual variation in the responses among the stallions and, further, libido was not totally suppressed.
Subject(s)
Gonadotropin-Releasing Hormone/immunology , Horses/physiology , Immunization/veterinary , Semen/physiology , Sexual Behavior, Animal/physiology , Testis/physiology , Animals , Antibodies/blood , Estrone/blood , Histocytochemistry/veterinary , Horses/immunology , Horses/psychology , Immunization/methods , Libido/physiology , Luminescent Measurements , Male , Microscopy, Electron/veterinary , Radioimmunoassay/veterinary , Scintillation Counting/veterinary , Sperm Motility/physiology , Testis/anatomy & histology , Testis/ultrastructure , Testosterone/bloodABSTRACT
Our objective was to examine the distribution of enterocyte digestive enzyme activity along the crypt-villus and longitudinal axes of the small intestine in formula-fed neonatal pigs between the ages of 14 and 18 d. The distended intestinal sac method was used to isolate 12 sequential fractions (F1 through F12) of epithelial cells. Enterocyte migration rate was measured in the proximal and distal intestine using in vivo bromodeoxyuridine labeling. Specific activities of representative villus cell marker enzymes of alkaline phosphatase, aminopeptidase N, sucrase, and lactase increased 6- to 17-fold from F12 (crypt cells) to F1 (villus cells), whereas the crypt cell marker [3H]thymidine incorporation increased 8- to 18-fold from F1 (villus cells) to F12 (crypt cells). Enterocyte migration rate was similar (3.2 vs 3.0 microm/h), whereas the villus height (547.4 vs 908.5 microm) and enterocyte life span (4.7 vs 10.2 d) were markedly lower (P < 0.05) in the proximal than in the distal segments, respectively. In general, the specific activities of all enzymes were lowest in the crypt fractions (F9 through F12) but increased markedly (ranging from 8- to 17-fold) from F12 to F1. The activity of aminopeptidase N was higher and that of sucrase was lower in the distal than in the proximal segment. The activities of the remaining enzymes were similar in the proximal and the distal segments. Our results suggest that the enterocyte life span in the distal small intestine is approximately twice as long as in the proximal small intestine. However, despite the difference in life span, the patterns of enzyme activities along the crypt-villus axis were generally similar in the proximal and the distal regions.
Subject(s)
Cation Transport Proteins , Intestinal Mucosa/enzymology , Intestine, Small/enzymology , Swine/metabolism , Adenosine Triphosphatases/metabolism , Alkaline Phosphatase/metabolism , Animals , Animals, Newborn , CD13 Antigens/metabolism , Cell Movement/physiology , Epithelial Cells/cytology , Epithelial Cells/enzymology , Histocytochemistry/veterinary , Image Processing, Computer-Assisted , Intestinal Absorption , Intestinal Mucosa/cytology , Intestinal Mucosa/growth & development , Intestine, Small/cytology , Intestine, Small/growth & development , Lactase , Microvilli/enzymology , Scintillation Counting/veterinary , Sodium-Potassium-Exchanging ATPase/metabolism , Sucrase/metabolism , Swine/growth & development , beta-Galactosidase/metabolismABSTRACT
The absorption, distribution and elimination of 14C-labelled flumequine were studied using whole body autoradiography and liquid scintillation counting. Flumequine was administered to eel Anguilla anguilla, turbot Scophthalmus maximus and halibut Hippoglossus hippoglossus intravenously and orally as a single dose of 5 mg kg(-1), corresponding to 0.1 mCi kg(-1). The turbot and halibut studies were performed in salt water (salinity of 32%) at temperatures of 16 +/- 1 degrees C (turbot) and 9.5 +/- 0.5 degrees C (halibut). The eel study was conducted in fresh water at 23 +/- 1 degrees C. In the intravenously administered groups flumequine was rapidly distributed to all major tissues and organs. After oral administration flumequine also appeared to have rapid and extensive absorption and distribution in all 3 species. After the distribution phase, the level of flumequine was higher in most organs and tissues than in the blood, except in muscle and brain. The most noticeable difference between the species was the slow elimination of flumequine from eel compared to turbot and halibut. In orally administered eels, substantial amounts of flumequine remained in all major organs/tissues for 7 d. At 28 d significant levels of flumequine were present in liver, kidney and skin (with traces in muscle), and at the last sampling point (56 d) in eye, bone, bile and posterior intestine. In orally administered turbot significant levels of flumequine were observed over 96 h in bile, urine, bone, skin, intestine and eye, and traces were detected over 28 d in bone and eye in addition to a significant level in bile. In orally administered halibut, significant levels of flumequine were observed in bile, skin, intestine and eye over 96 h. Traces were present in skin and eye over 7 d. The maximal flumequine concentrations in blood were calculated to be 2.5 mg equivalents l(-1) (eel at 12 h), 0.8 mg l(-1) (turbot at 6 h) and 0.6 mg l(-1) (halibut at 6 h) after oral administration.
Subject(s)
Anguilla/metabolism , Anti-Infective Agents/pharmacokinetics , Flatfishes/metabolism , Flounder/metabolism , Fluoroquinolones , Quinolizines/pharmacokinetics , Absorption , Administration, Oral , Animals , Anti-Infective Agents/administration & dosage , Autoradiography/veterinary , Carbon Isotopes , Injections, Intravenous/veterinary , Quinolizines/administration & dosage , Scintillation Counting/veterinary , Species Specificity , Tissue DistributionABSTRACT
The effect of Clinostomum detruncatum metacercaria infection on the activities of the antioxidant enzymes superoxide dismutase and catalase in muscle of the freshwater fish Rhamdia quelen was analyzed. Tert-butyl hydroperoxide-initiated chemiluminescence, a measure of lipid peroxidation, was also investigated. Enzyme activities were similar in infected and uninfected fishes. However, the chemiluminescence was almost 2-fold higher in muscle of infected fishes than in muscle of uninfected ones. These results indicate that parasite infection induces oxidative stress and a higher level of membrane damage in the fish muscle due to an imbalance between pro-oxidants and non-enzymatic antioxidants. Our results suggest that fish response to parasite infection could involve, as in other vertebrates, reactive oxygen intermediates.
Subject(s)
Catfishes , Fish Diseases/parasitology , Lipid Peroxidation , Muscle, Skeletal/parasitology , Trematoda/pathogenicity , Trematode Infections/veterinary , Animals , Brazil , Catalase/analysis , Fish Diseases/pathology , Luminescent Measurements , Muscle, Skeletal/enzymology , Muscle, Skeletal/pathology , Oxidative Stress , Scintillation Counting/veterinary , Superoxide Dismutase/analysis , Trematode Infections/parasitology , Trematode Infections/pathology , tert-Butylhydroperoxide/chemistryABSTRACT
Anaplasma marginale is the etiological agent of anaplasmosis, a tick-transmitted disease with an important economic impact that affects cattle throughout the world. Although, North American isolates of A. marginale and their antigens have been extensively studied, relatively little information is available on the antigenic composition of South American isolates. The characterization of diverse geographical isolates of A. marginale will result in a thorough antigenic profile and may lead to the identification of additional diagnostic and immunoprophylactic tools. Short-term cultures of a Venezuelan isolate (Ta) of A. marginale were maintained for up to 13 days in vitro. During that period, the A. marginale remained viable and were propagated in the bovine erythrocyte culture system. During the initial days of culture, cell division and reinvasion were evidenced by a significant rise in parasitemia up to a 50%. A. marginale antigens were identified by metabolic labeling with (35S) methionine, followed by fractionation and immunoprecipitation with homologous and heterologous bovine sera. This yielded a complete antigenic set for the Ta isolate of A. marginale, including soluble, secreted and corpuscular polypeptide antigens. Fifteen immunodominant polypeptides were recognized by the bovine sera in the soluble and corpuscular fractions with relative molecular weights of 200, 150, 100-110, 86, 60, 50, 47, 40, 37, 33, 31, 25, 23, 19 and 16kDa. Seven polypeptides were present in the exoantigen fraction. The 31 and 19kDa antigens were recognized by the ANAR76A1 and ANAF16C1 monoclonal antibodies, respectively which are specific for MSP-4 and MSP-5 from North American isolates of A. marginale. Metabolic labeling with (14C) glucosamine prior to immunoprecipitation with bovine sera allowed the identification of glycoprotein antigens of 200, 100-150, 60, 55, 50, 45-43, 37, 33, 31, 22, 19 and 16kDa in the soluble fraction.
Subject(s)
Anaplasma/immunology , Anaplasmosis/immunology , Antigens, Bacterial/analysis , Anaplasmosis/microbiology , Animals , Antibodies, Bacterial/blood , Antibodies, Monoclonal , Antigens, Bacterial/chemistry , Bacteremia/microbiology , Bacteremia/veterinary , Carbon Radioisotopes , Cattle , Cell Culture Techniques , Enzyme-Linked Immunosorbent Assay/veterinary , Male , Molecular Weight , Precipitin Tests/veterinary , Scintillation Counting/veterinary , Sulfur Radioisotopes , VenezuelaABSTRACT
Bovine morulae and blastocysts were either produced in vitro through maturation, fertilization and culture of immature oocytes recovered from slaughterhouse-derived ovaries, collected in vivo or obtained after 24 h in vitro culture of in vivo collected embryos. The morulae and blastocysts were classified into four categories of embryo quality and two stages of embryonic development. Embryos were frozen by a controlled freezing method using 10% glycerol as a cryoprotectant. The ability of individual embryos to withstand freeze/thawing was measured immediately before and after cryopreservation by changes in CO2 production from (U-14C)glucose during a 2 h incubation period in a non-invasive closed system immediately before and after cryopreservation. Post-thaw survival was assessed by development in vitro during a 48 h culture period. Survival rates and oxidative metabolism after freeze/thawing were similar in embryos of the two developmental stages. However, after freeze/thawing, the rate of CO2 production of in vitro produced embryos was reduced to one half of their pre-freeze levels and was associated with poor survival rates. In vivo collected embryos had a significantly better tolerance to freezing and higher survival rates. However, when in vivo embryos were exposed to in vitro culture conditions, the rates of CO2 production and survival were significantly reduced. Pre-freeze embryo quality affected post-thaw in vitro development and metabolic activity markedly in embryos produced in vitro or pre-exposed to in vitro culture conditions. While there was no relationship between pre-freeze levels of CO2 production and post-thaw in vitro embryo development, all embryos which developed in vitro after freezing/thawing retained at least 58% of the pre-freeze levels of CO2 production regardless of their origin. Results of the present study indicate that embryos produced in vitro or pre-exposed to in vitro culture conditions are more sensitive to cryo-injury. This sensitivity is affected by embryo quality and is similarly reflected at the biochemical level. Determination of oxidative metabolism offers a feasibility for selection of viable morulae/blastocysts after freezing/thawing.
Subject(s)
Blastocyst/metabolism , Cattle/physiology , Cryopreservation/veterinary , Cryoprotective Agents/adverse effects , Glucose/metabolism , Morula/metabolism , Animals , Blastocyst/physiology , Carbon Dioxide/analysis , Carbon Radioisotopes , Cryopreservation/methods , Embryonic and Fetal Development , Female , Fertilization in Vitro/methods , Fertilization in Vitro/veterinary , Glycerol/adverse effects , Male , Morula/physiology , Ovarian Follicle/physiology , Scintillation Counting/veterinary , Superovulation/physiologyABSTRACT
Our objective was to determine if thiopurine methyltransferase (TPMT), the enzyme important in the metabolism of azathioprine in human beings, is detectable in red blood cell lysates (RBCL) of healthy dogs, cats, and horses. Values for TPMT activity were determined from blood collected from 20 healthy dogs, cats, and horses. The TPMT activity in each animal's RBCL was determined using a radioenzymatic end point involving TPMT-facilitated metabolism of 6-mercaptopurine to 6-methylmercaptopurine (6-MMP). One unit of TPMT activity represents the formation of 1 nmol of 6-MMP per milliliter of packed red blood cells per hour of incubation at 37 degrees C. TPMT activity in RBCL was detectable in all species, with mean RBC values +/- standard deviation of 17.9 +/- 3.79 U/mL in dogs; 2.76 +/- 0.70 U/mL in cats; and 2.185 +/- 0.36 U/mL in horses. Values for TPMT in the 3 species were significantly (P < .05) different from one another. TPMT values for dogs were significantly higher than the other species, and TPMT values for cats were significantly higher than those for horses. We conclude that RBCL TPMT values are measurable in dogs. cats, and horses and that dogs have higher values than cats or horses. These findings are consistent with the lower tolerance for azathioprine in cats as compared with dogs. It remains to be determined whether RBCL TPMT values in these species correlate with TPMT activity in the liver, where most of the metabolization of azathioprine is believed to occur.
Subject(s)
Cats/physiology , Dogs/physiology , Erythrocytes/enzymology , Horses/physiology , Mercaptopurine/analogs & derivatives , Methyltransferases/blood , Animals , Azathioprine/chemistry , Cats/blood , Dogs/blood , Female , Horses/blood , Immunosuppressive Agents/chemistry , Male , Mercaptopurine/analysis , Reference Values , Scintillation Counting/veterinaryABSTRACT
Azathioprine is a purine analogue used as an immunosuppressive and immunomodulator agent in various mammals, including cats. Several adverse reactions have been reported and have limited the use of the drug in the cat. Adverse reactions to azathioprine in humans have been correlated with reduced activity of thiopurine methyltransferase (TPMT) in erythrocytes. The purpose of this preliminary study was to determine if cats have TPMT activity in their erythrocytes and to compare the values obtained with the normal range for humans and the normal range for dogs in a preliminary report. Activity of the enzyme was measured in blood samples drawn from 41 cats. Blood also was taken from 5 dogs. The mean erythrocyte TPMT activity in the cats was 2.4 +/- 0.4 nmol (range, 1.2-3.9 nmol) per hour per milliliter of red blood cells (U/mL RBC) or 2-8 nmol per hour per gram of hemoglobin (U/g Hb). This range was far lower than the normal human range (8-15 U/mL RBC; 16-33 U/g Hb) and was of monopolar distribution. This observation apparently precludes any diagnostic purpose in assaying erythrocyte TPMT in this species. Erythrocyte TPMT activity in the 5 dogs ranged from 5.5 to 13.1 U/mL RBC (11-27 U/g Hb), which was comparable with normal and carrier ranges for humans, but proof of TPMT genetic polymorphism in either species will require genotyping studies.
Subject(s)
Cats/physiology , Erythrocytes/enzymology , Methyltransferases/blood , Animals , Azathioprine/adverse effects , Azathioprine/therapeutic use , Cats/blood , Dogs , Female , Humans , Immunosuppressive Agents/adverse effects , Immunosuppressive Agents/therapeutic use , Male , Reference Values , Scintillation Counting/veterinaryABSTRACT
The trophoblast has a significant role in regulation of immune reactions at the materno-fetal interface by producing biologically active substances. In our previous studies five fractions with immunomodulatory activities were isolated by gel chromatography from trophoblast of pig placentas. To confirm the immunomodulatory effect of these trophoblast fractions on allogeneic in vivo systems and to obtain more evidence for the relevance of their activity on the maternofetal interface, their effect was studied on graft-versus-host reaction (GVHR). To assess the GVHR, the primary and secondary popliteal lymph nodes assay was used in mice. In the primary GVHR, 100 microg protein of Fraction 2-5, mixed with 5 x 10(6) allogeneic spleen cells (C57BL/6), were injected into one of the foot pads of recipient (BALB/c) mice. The secondary GVHR was induced in F1 (BALB/c x C57BL/6) mice by injection of spleen cells of BALB/c mice intraperitoneally preimmunized with allogeneic cells. The GVHR was measured by the weight of lymph nodes and by the lymphocyte proliferation. Flow cytometric analyses of the cells in the nodes with GVHR and under the influence of Fraction 4 or 5 were performed using monoclonal antibodies. In the primary GVHR, Fraction 4 or 5, injected simultaneously with allogeneic spleen cells, significantly suppressed the lymph nodes reactivity. Fractions 4 and 5 inhibited the ability of the spleen cells of mice intraperitoneally preimmunized with allogeneic cells to induce secondary GVHR in F1 mice. The Fraction 2 and 3 had no effect on GVHR. The results revealed that a group of proteins with Mr 37-7 kDa, isolated from trophoblast of pig placenta, strongly suppressed popliteal lymph node reactivity in the primary and secondary GVHR. The data provide convincing evidence for these fractions in vivo activity, for their effect across the species barrier and suggest the relevance of the same reactions on the materno-fetal interface.
Subject(s)
Graft vs Host Reaction/immunology , Swine/immunology , Trophoblasts/immunology , Animals , Antibodies, Monoclonal , Biological Assay , Cell Division , Chromatography, Gel/veterinary , Crosses, Genetic , Female , Flow Cytometry/veterinary , Graft vs Host Reaction/physiology , Lymph Nodes/immunology , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Pregnancy , Scintillation Counting/veterinary , Specific Pathogen-Free Organisms , Statistics, Nonparametric , Swine/physiology , Thymidine/chemistry , Trophoblasts/physiologyABSTRACT
OBJECTIVES: To study the in vitro effects of cecal contents incubated with corn starch on colonic permeability in horses. ANIMALS: 4 healthy adult ponies. PROCEDURE: Mucosal specimens were obtained from the right ventral colon and mounted in Ussing chambers. Changes in short circuit current, conductance, and large-molecule permeability in response to addition of cecal contents and cecal contents incubated with corn starch were evaluated for 120 minutes. RESULTS: Incubation of cecal contents with corn starch for 8 hours resulted in a decrease in cecal content pH and an increase in lactic acid concentration. These changes were similar to those reported in vivo for ponies given corn starch. Exposure of colonic mucosa to cecal contents incubated with corn starch resulted in an increase in tissue conductance and permeability of technetium Tc 99m pentetate, compared with mucosa exposed to cecal contents alone. CONCLUSIONS AND CLINICAL RELEVANCE: In vitro exposure of colonic mucosa to cecal contents incubated with starch resulted in increased paracellular permeability. Fermentation of excessive amounts of carbohydrate in the intestinal lumen of horses may directly induce increased intestinal permeability associated with carbohydrate-induced laminitis.
Subject(s)
Cecum/physiopathology , Colon/metabolism , Gastrointestinal Diseases/veterinary , Horse Diseases/physiopathology , Intestinal Mucosa/physiopathology , Animals , Cecum/metabolism , Electrophysiology , Gastrointestinal Contents/chemistry , Gastrointestinal Diseases/etiology , Gastrointestinal Diseases/physiopathology , Horse Diseases/etiology , Horses , Hydrogen-Ion Concentration , Intestinal Mucosa/metabolism , Lactic Acid/analysis , Osmolar Concentration , Permeability , Scintillation Counting/veterinary , Starch/metabolism , Technetium Tc 99m Pentetate/chemistryABSTRACT
OBJECTIVE: To compare the in vitro immunosuppressive effects of cyclosporine and 4 novel immunosuppressive drugs on lymphocytes in whole blood collected from healthy cats. SAMPLE POPULATION: Whole blood samples collected from 10 healthy adult domestic shorthair cats. PROCEDURE: Mitogen-stimulated lymphocyte proliferation in whole blood incubated with and without various concentrations of cyclosporine, tacrolimus, sirolimus, mycophenolic acid (MPA), or A771726 was measured by use of [3H]thymidine incorporation. Drug concentrations that resulted in a 50% inhibition of mitogen-induced proliferation (IC50) were calculated. Lymphocyte viability was determined by use of the trypan blue dye exclusion method. RESULTS: An obvious dose-response relationship for the antiproliferative effects of each drug was detected. Mean IC50 determined with concanavalin A was 46 nM for cyclosporine, 9 nM for tacrolimus, 12 nM for sirolimus, 16 nM for MPA, and 30 mM for A771726, whereas with pokeweed mitogen, mean IC50 was 33 nM for cyclosporine, 5 nM for tacrolimus, 15 nM for sirolimus, 14 nM for mycophenolic acid, and 25 mM for A771726. Mitogen-stimulated and nonstimulated lymphocytes remained viable, regardless of drug evaluated. CONCLUSIONS AND CLINICAL RELEVANCE: Tacrolimus, sirolimus, MPA, and A771726 inhibited in vitro mitogen-stimulated proliferation of feline lymphocytes in a dose-dependent manner. These novel immunosuppressive drugs may be useful for management of immune-mediated inflammatory diseases and prevention and treatment of rejection in cats that undergo organ transplantation.
Subject(s)
Cats/immunology , Immunosuppressive Agents/pharmacology , Lymphocyte Activation/drug effects , Animals , Cell Division/drug effects , Cell Division/immunology , Coloring Agents/chemistry , Concanavalin A/chemistry , Cyclosporine/pharmacology , Dose-Response Relationship, Drug , Inhibitory Concentration 50 , Isoxazoles/pharmacology , Leflunomide , Lymphocyte Activation/immunology , Mycophenolic Acid/pharmacology , Pokeweed Mitogens/chemistry , Scintillation Counting/veterinary , Sirolimus/pharmacology , Tacrolimus/pharmacology , Trypan Blue/chemistryABSTRACT
OBJECTIVE: To compare cytokine secretion patterns of peripheral blood mononuclear cells (PBMC) from healthy cows and cows subclinically and clinically infected with Mycobacterium paratuberculosis. ANIMALS: 5 noninfected cows, 6 cows with subclinical paratuberculosis, and 4 cows with clinical paratuberculosis. PROCEDURE: PBMC were isolated, and concentrations or activities of secreted interleukin (IL)-1, IL-2, IL-6, tumor necrosis factor (TNF), and interferon-gamma (IFN-gamma) were measured after in vitro stimulation of cells with concanavalin A (ConA), lipopolysaccharide (LPS), or a whole-cell sonicate of M paratuberculosis (MpS). Proliferative responses of PBMC were also determined after stimulation with ConA, phytohemagglutinin, pokeweed mitogen (PWM), or MpS. RESULTS: After stimulation with ConA, cells from subclinically infected cows secreted significantly more, and cells from clinically infected cows secreted significantly less, IFN-gamma, compared with cells from control cows. Cells from cows with subclinical paratuberculosis produced significantly more TNF and IFN-gamma in response to MpS than cells from the other 2 groups. Stimulation of PBMC from subclinically infected cows with ConA or MpS resulted in significantly higher proliferative responses, compared with cells from control and clinically infected cows. In contrast, clinically infected cows had significantly higher proliferative responses to PWM than cells from the other 2 groups. CONCLUSIONS AND CLINICAL RELEVANCE: A decrease in T-cell responses to mitogens or MpS was observed in cows clinically infected with M paratuberculosis, compared with subclinically infected cows, suggesting that activated T cells may delay the progression of paratuberculosis.
Subject(s)
Cattle Diseases/immunology , Cytokines/metabolism , Leukocytes, Mononuclear/metabolism , Mycobacterium avium subsp. paratuberculosis/immunology , Paratuberculosis/immunology , Animals , Cattle , Cattle Diseases/microbiology , Cell Division , Concanavalin A/immunology , Cytokines/analysis , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Interferon-gamma/analysis , Interleukin-1/analysis , Interleukin-2/analysis , Leukocytes, Mononuclear/immunology , Lipopolysaccharides/immunology , Lymphocyte Activation , Mycobacterium avium subsp. paratuberculosis/pathogenicity , Paratuberculosis/blood , Paratuberculosis/microbiology , Phytohemagglutinins/immunology , Pokeweed Mitogens/immunology , Scintillation Counting/veterinaryABSTRACT
It has been accepted for many years that the susceptibility of the genital tract to infection is reduced during the follicular phase compared with the luteal phase of the estrous cycle. Since the role of intrauterine neutrophils is paramount in the elimination of bacteria, it can be hypothesized that these differences in resistance to infection could be mediated by differences in uterine-derived neutrophil function. In order to test this hypothesis two groups of cows were used in this study. Group 1 cows (n=5) were studied at estrus, diestrus, after ovariectomy, after exogenous estradiol and after progesterone treatment, at which time they underwent intrauterine infusion with 1% oyster glycogen (OG) and a bacterial-free filtrate (BFF) of Actinomyces genes (BFF), the latter having been recovered from a clinical case of endometritis; neutrophils were harvested by flushing from the lumen 15 to 18 h later. A peripheral blood sample was collected at the time of flushing for the assay of estradiol and progesterone for a WBC and differential count and for the harvesting of neutrophils using a Percoll single-stage discontinuous gradient. After the recovery of the cells they were re-suspended in HBSS. Group 2 (n=4) were infused with BFF during during all reproductive states as Group 1, but with OG only after ovariectomy and after treatment with progesterone and estradiol. Neutrophil chemotaxis was assessed by measuring their migration using a modified Boyden chamber and Zymogen-activated serum as a chemoattractant. Phagocytic activity was measured by determining the number of Candida albicans ingested by each neutrophil after incubation. The percentage of kill was determined using a radiometric assay in which C. albicans was labeled with L-(5-3H) Proline. Peripheral WBC concentration was not influenced by the reproductive state of the cow; however, the mean neutrophil concentration was significantly different between the reproductive states (P<0.001) and between individual cows (P<0.001). In Group 1, there was little difference in the function of the peripheral and uterine neutrophils, and while there were differences in all 3 aspects of neutrophil function from both sources between reproductive states and individual cows, of which some were statistically significant, there was no consistent pattern. In Group 2, neutrophils recovered after the infusion of BFF had poorer function compared with those recovered after the infusion of OG. There was no consistent influence of the reproductive state or individual animal. The hypothesis that the influence of the reproductive state of the cow on the resistance of the uterus to infection is mediated by the inherent differences in either peripheral or intrauterine neutrophil function was not supported by this study.