Dynamics of seaweed-inspired piezoelectric plates for energy harvesting from oscillatory cross flow.

Inspired by the vibrations of aquatic plants such as seaweed in the unsteady flow fields generated by free-surface waves, we investigate a novel device based on piezoelectric plates to harvest energy from oscillatory cross flows. Towards this end, numerical studies are conducted using a flow-structure-electric interaction model to understand the underlying physical mechanisms involved in the dynamics and energy harvesting performance of one or a pair of piezoelectric plates in an oscillatory cross flow. In a single-plate configuration, both periodic and irregular responses have been observed depending on parameters such as normalized plate stiffness and Keulegan-Carpenter number. Large power harvesting is achieved with the excitation of natural modes. Besides, when the time scale of the motion and the intrinsic time scale of the circuit are close to each other the power extraction is enhanced. In a two-plate configuration with tandem formation, the hydrodynamic interaction between the two plates can induce irregularity in the response. In terms of energy harvesting, two counteracting mechanisms have been identified, shielding and energy recovery. The shielding effect reduces plate motion and energy harvesting, whereas with the energy recovery effect one plate is able to recovery energy from the wake of another for performance enhancement. The competition between these mechanisms leads to constructive or destructive interactions between the two plates. These results suggest that for better performance the system should be excited at its natural period, which should be close to the intrinsic time scale of the circuit. Moreover, using a pair of plates in a tandem formation can further improve the energy harvesting capacity when conditions for constructive interaction are satisfied.

Changes in maerl-associated macroalgal community dynamics as evidence of anthropogenic pressure.

BACKGROUND AND AIMS: Maerl-associated communities have received considerable attention due to their uniqueness, biodiversity and functional importance. Although the impacts of human activities are well documented for maerl-associated macrofauna, the spatio-temporal variations of macroalgae have comparatively been neglected, and the drivers that influence their dynamics are poorly known. We investigate the links between maerl-associated macroalgal communities, anthropogenic pressures and environmental conditions, and hypothesize that sites under human pressure would exhibit different dynamics when compared to reference sites. METHODS: To better understand community variation through space and time, four subtidal maerl beds under different pressures were consistently monitored over one year in the bay of Brest, Brittany, France. Both macroalgae community monitoring and environmental data were acquired through field sampling and available models. KEY RESULTS: Higher macroalgal biomass was observed within eutrophic sites, especially in summer (more than ten times higher than in the Unimpacted site), caused by free-living forms of opportunistic red macroalgae. The Dredged site also exhibited distinct macroalgal communities during summer from the Unimpacted site. Nutrient concentrations and seasonality proved to be key factors affecting the macroalgal community composition, although dredging and its effects on granulometry also had a strong influence. Over the long term, fewer than half of the species identified during historical surveys were found, indicating major temporal changes. CONCLUSIONS: Human pressures have strong impacts on maerl-associated macroalgal communities. Nutrient concentrations and dredging pressure appear as the main anthropogenic factors shaping maerl-associated macroalgal communities. Additionally, our results suggest historical changes in maerl-associated macroalgal communities over 25 years in response to changes in local human pressure management. This study suggests that maerl-associated macroalgal communities could be used as indicators of anthropogenically driven changes in this habitat.

Victim of changes? Marine macroalgae in a changing world.

BACKGROUND: Marine macroalgae ('seaweeds') are a diverse and globally distributed group of photosynthetic organisms that together generate considerable primary productivity, provide an array of different habitats for other organisms, and contribute many important ecosystem functions and services. As a result of continued anthropogenic stress on marine systems, many macroalgal species and habitats face an uncertain future, risking their vital contribution to global productivity and ecosystem service provision. SCOPE: After briefly considering the remarkable taxonomy and ecological distribution of marine macroalgae, we review how the threats posed by a combination of anthropogenically induced stressors affect seaweed species and communities. From there we highlight five critical avenues for further research to explore (long-term monitoring, use of functional traits, focus on early ontogeny, biotic interactions and impact of marine litter on coastal vegetation). CONCLUSIONS: Although there are considerable parallels with terrestrial vascular plant responses to the many threats posed by anthropogenic stressors, we note that the impacts of some (e.g. habitat loss) are much less keenly felt in the oceans than on land. Nevertheless, and in common with terrestrial plant communities, the impact of climate change will inevitably be the most pernicious threat to the future persistence of seaweed species, communities and service provision. While understanding macroalgal responses to simultaneous environmental stressors is inevitably a complex exercise, our attempt to highlight synergies with terrestrial systems, and provide five future research priorities to elucidate some of the important trends and mechanisms of response, may yet offer some small contribution to this goal.

Split-plot marine experiment to assess ecophysiological responses of Gelidium corneum assemblages.

Canopy-forming macroalgae are facing large declines due to climate change worldwide. The foundation species Gelidium corneum (Hudson) J.V. Lamouroux has shown a long-term decline in the Southeastern Bay of Biscay. We conducted an in situ experiment to investigate the combined effect of solar radiation and nutrient availability on the photosynthetic acclimation and growth of this macrophyte, and on the species richness and diversity of the assemblages that it forms. Photochemical stress in G. corneum was found to be greater at the end of the study, probably as a result of a prolonged exposure to high irradiance (PAR and UVR) and due to high temperatures during summer. We found an acclimation of G. corneum specimens to summer light and thermal conditions through dynamic/reversible photoinhibition and a decrease in photosynthetic efficiency. Nutrients may also have had a positive effect in dealing with the negative effects of these stressors. Under ongoing global climate change and projections for the future, further research will be needed to better understand the effects not only on canopy forming species but also on the whole community and thus on the functioning of the ecosystem.

Competitive effects of the macroalga Caulerpa taxifolia on key physiological processes in the scleractinian coral Turbinaria peltata under thermal stress.

An increased abundance of macroalgae has been observed in coral reefs damaged by climate change and local environmental stressors. Macroalgae have a sublethal effect on corals that includes the inhibition of their growth, development, and reproduction. Thus, this study explored the effects of the macroalga, Caulerpa taxifolia, on the massive coral, Turbinaria peltata, under thermal stress. We compared the responses of the corals' water-meditated interaction with algae (the co-occurrence group) and those in direct contact with algae at two temperatures. The results show that after co-culturing with C. taxifolia for 28 days, the density content of the dinoflagellate endosymbionts was significantly influenced by the presence of C. taxifolia at ambient temperature (27 °C), from 1.3 × 106 cells cm-2 in control group to 0.95 × 106 cells cm-2 in the co-occurrence group and to 0.89 × 106 cells cm-2 in the direct contact group. The chlorophyll a concentration only differed significantly between the control and the direct contact group at 27 °C. The protein content of T. peltata decreased by 37.2% in the co-occurrence group and 49.0% in the direct contact group compared to the control group. Meanwhile, the growth rate of T. peltata decreased by 57.7% in the co-occurrence group and 65.5% in the direct contact group compared to the control group. The activity of the antioxidant enzymes significantly increased, and there was a stronger effect of direct coral contact with C. taxifolia than the co-occurrence group. At 30 °C, the endosymbiont density, chlorophyll a content, and growth rate of T. peltata significantly decreased compared to the control temperature; the same pattern was seen in the increase in antioxidant enzyme activity. Additionally, when the coral was co-cultured with macroalgae at 30 °C, there was no significant decrease in the density or chlorophyll a content of the endosymbiont compared to the control. However, the interaction of macroalgae and elevated temperature was evident in the feeding rate, protein content, superoxide dismutase (SOD), and catalase (CAT) activity compared to the control group. The direct contact of the coral with macroalga had a greater impact than water-meditated interactions. Hence, the competition between coral and macroalga may be more intense under thermal stress.

Short-term response of macroalgal communities to ocean warming in the Southern Bay of Biscay.

Climate change is causing significant shifts in biological communities worldwide, including the degradation of marine communities. Previous research has predicted that southern Bay of Biscay canopy-forming subtidal macroalgal communities will shift into turf-forming Mediterranean-like communities by the end of the century. These predictions were based on a community-environment relationship model that used macroalgal abundance data and IPCC environmental projections. We have tested the short-term accuracy of that model by resampling the same communities and locations four years later and found the short-term predictions to be consistent with the observed communities. Changes in sea surface temperature were positively correlated with changes in the Community Temperature Index, suggesting that macroalgal communities had responded quickly to global warming. The changes over four years were significant, but canopy-forming macroalgae were more resilient in local sites with favourable temperature conditions. Our study demonstrated that updating predictive models with new data has the potential to yield reliable predictions and inform effective conservation strategies.

Macroalgae exhibit diverse responses to human disturbances on coral reefs.

Scientists and managers rely on indicator taxa such as coral and macroalgal cover to evaluate the effects of human disturbance on coral reefs, often assuming a universally positive relationship between local human disturbance and macroalgae. Despite evidence that macroalgae respond to local stressors in diverse ways, there have been few efforts to evaluate relationships between specific macroalgae taxa and local human-driven disturbance. Using genus-level monitoring data from 1205 sites in the Indian and Pacific Oceans, we assess whether macroalgae percent cover correlates with local human disturbance while accounting for factors that could obscure or confound relationships. Assessing macroalgae at genus level revealed that no genera were positively correlated with all human disturbance metrics. Instead, we found relationships between the division or genera of algae and specific human disturbances that were not detectable when pooling taxa into a single functional category, which is common to many analyses. The convention to use percent cover of macroalgae as an indication of local human disturbance therefore likely obscures signatures of local anthropogenic threats to reefs. Our limited understanding of relationships between human disturbance, macroalgae taxa, and their responses to human disturbances impedes the ability to diagnose and respond appropriately to these threats.

Warming and nutrient enrichment can trigger seaweed loss by dysregulation of the microbiome structure and predicted function.

Warming and nutrient enrichment are key pervasive drivers of ecological shifts in both aquatic and terrestrial ecosystems, impairing the physiology and survival of a wide range of foundation species. But the underlying mechanisms often remain unclear, and experiments have overlooked the potential effects mediated by changes in the microbial communities. We experimentally tested in the field orthogonal stress combinations from simulated air warming and nutrient enrichment on the intertidal foundation seaweed Cystoseira compressa, and its associated bacterial communities. A total of 523 Amplicon Sequence Variance (ASVs) formed the bacterial community on C. compressa, with 222 ASVs assigned to 69 taxa at the genus level. Most bacteria taxa experienced changes in abundance as a result of additive (65 %) and antagonistic (30 %) interactions between the two stressors, with synergies (5 %) occurring less frequently. The analysis of the predicted bacterial functional profile identified 160 metabolic pathways, and showed that these were mostly affected by additive interactions (74 %) between air warming and nutrient enrichment, while antagonisms (20 %) and synergisms (6 %) were less frequent. Overall, the two stressors combined increased functions associated with seaweed disease or degradation of major cell-wall polymers and other algicidal processes, and decreased functions associated with Quorum Quenching and photosynthetic response. We conclude that warming and nutrient enrichment can dysregulate the microbiome of seaweeds, providing a plausible mechanism for their ongoing loss, and encourage more research into the effects of human impacts on crucial but yet largely unstudied host-microbiome relationships in different aquatic and terrestrial species.

Climate change and species facilitation affect the recruitment of macroalgal marine forests.

Marine forests are shrinking globally due to several anthropogenic impacts including climate change. Forest-forming macroalgae, such as Cystoseira s.l. species, can be particularly sensitive to environmental conditions (e.g. temperature increase, pollution or sedimentation), especially during early life stages. However, not much is known about their response to the interactive effects of ocean warming (OW) and acidification (OA). These drivers can also affect the performance and survival of crustose coralline algae, which are associated understory species likely playing a role in the recruitment of later successional species such as forest-forming macroalgae. We tested the interactive effects of elevated temperature, low pH and species facilitation on the recruitment of Cystoseira compressa. We demonstrate that the interactive effects of OW and OA negatively affect the recruitment of C. compressa and its associated coralline algae Neogoniolithon brassica-florida. The density of recruits was lower under the combinations OW and OA, while the size was negatively affected by the temperature increase but positively affected by the low pH. The results from this study show that the interactive effects of climate change and the presence of crustose coralline algae can have a negative impact on the recruitment of Cystoseira s.l. species. While new restoration techniques recently opened the door to marine forest restoration, our results show that the interactions of multiple drivers and species interactions have to be considered to achieve long-term population sustainability.

Effect of UV-radiation on the physiology of the invasive green seaweed Codium fragile and its associated bacteria.

Invasive species such as seaweeds often have a broad tolerance, allowing them to colonize novel habitats. During invasion, also new epibacteria can be formed on seaweeds, which have important chemo-ecological effects. Since UV-radiation (UVR) is one of the main factors affecting seaweeds and their epibacteria, we tested its effect on intertidal and subtidal thalli of the invasive seaweed Codium fragile from three sites and monitored photosynthesis, antioxidant activity and epibacteria. Exposure to UV-radiation resulted in photoinhibition with a subsequent low recovery in subtidal thalli from 23°S compared to 27°S and 30°S, which both showed a higher and almost complete recovery. However, a high antioxidant activity was present in all thalli, permitting to explain its relatively high tolerance to new environments. UV-radiation modified the composition of the epibacteria community by reducing its diversity and evenness. Our results showed that C. fragile responds plastic to variable UV-radiation (depending on site and water depth), which contributes to its high invasion potential.

Pollinators of the sea: A discovery of animal-mediated fertilization in seaweed.

The long-held belief that animal-mediated pollination is absent in the sea has recently been contradicted in seagrasses, motivating investigations of other marine phyla. This is particularly relevant in red algae, in which female gametes are not liberated and male gametes are not flagellated. Using experiments with the isopod Idotea balthica and the red alga Gracilaria gracilis, we demonstrate that biotic interactions dramatically increase the fertilization success of the alga through animal transport of spermatia on their body. This discovery suggests that animal-mediated fertilization could have evolved independently in terrestrial and marine environments and raises the possibility of its emergence in the sea before plants moved ashore.

Did pollination exist before plants?

Research shows that seaweeds depend on crustaceans for fertilization.

[Analysis of the underlying mechanisms of green tide with a perspective of algae ecophysiology]. / ä»Žè—»ç±»ç”Ÿç†ç”Ÿæ€å­¦è§’åº¦è§£æžç»¿æ½®çˆ†å‘çš„å† åœ¨æœºåˆ¶.

Green tide in the sea is an abnormal ecological phenomenon caused by the explosive proliferation or high aggregation of some green macroalgae under particular environmental conditions. Since 2007, green tides have occurred in the Yellow Sea for 15 consecutive years, resulting in extremely serious damage to coastal ecological environment, social development, and economic construction. Therefore, it is urgent to solve the green tide problem, which is driven by external and internal factors. Global researchers have scientifically recognized the external causes (environmental factors) of the green tide blooms, and have carried out a series of studies on the physiological response of green tide algae to some environmental factors and obtained some achievements. However, the internal causes of green tide blooms, which is the intense ecophysiological adaptability of green tide algae in response to drastic fluctuation of environmental factors, has not yet been sufficiently addressed. From the perspective of algae ecophysiology, we reviewed the response mechanisms of green tide forming species to the fluctuations of various ecological factors, including light intensity, carbon dioxide, temperature, salinity, desiccation, nutrient, heavy metals, and biotic factors. Moreover, we summarized the adaptive regulation mechanisms of green tide algae dealing with fluctuating environmental factors from the aspects of photosynthetic and growth physiology, nutritional physio-logy, and reproductive physiology, which help reveal the internal mechanisms of green tide blooming.

Potential negative effects of ocean afforestation on offshore ecosystems.

Our scientific understanding of climate change makes clear the necessity for both emission reduction and carbon dioxide removal (CDR). The ocean with its large surface area, great depths and long coastlines is central to developing CDR approaches commensurate with the scale needed to limit warming to below 2 °C. Many proposed marine CDR approaches rely on spatial upscaling along with enhancement and/or acceleration of the rates of naturally occurring processes. One such approach is 'ocean afforestation', which involves offshore transport and concurrent growth of nearshore macroalgae (seaweed), followed by their export into the deep ocean. The purposeful occupation for months of open ocean waters by macroalgae, which do not naturally occur there, will probably affect offshore ecosystems through a range of biological threats, including altered ocean chemistry and changed microbial physiology and ecology. Here, we present model simulations of ocean afforestation and link these to lessons from other examples of offshore dispersal, including rafting plastic debris, and discuss the ramifications for offshore ecosystems. We explore what additional metrics are required to assess the ecological implications of this proposed CDR. In our opinion, these ecological metrics must have equal weight to CDR capacity in the development of initial trials, pilot studies and potential licensing.

Controlling the main source of green tides in the Yellow Sea through the method of biological competition.

Macroalgal blooms have become a serious threat to public health, fisheries, ecosystems, and global economies. Since 2007, in the Yellow Sea, China, Ulva green tides have occurred for 15 consecutive years. However, effective control methods are limited. Ulva prolifera attached to Neopyropia aquaculture rafts are believed to be the main source of blooms, therefore eliminating Ulva from rafts could effectively prevent and control blooms. We investigated this phenomenon and showed that macroalgae germination was significantly inhibited by dried Neopyropia yezoensis at concentrations of 1.2, 2.4, and 4.8 g DW-1. Also, the inhibitory effects of dried N. yezoensis toward U. prolifera gametes at 2.4 and 4.8 g DW-1 were >90% at day 21. N. yezoensis culture filtrates and thalli were also used to determine dose-dependent inhibition effects on U. prolifera gamete germination. Both were potent and significantly inhibited germination at 1.75-7 g FW-1; the inhibitory effect 7 g FW-1 was >90% at day 21. As N. yezoensis thalli exhibited high inhibitory effects in laboratory experiments, we also performed field studies. N. yezoensis on ropes displayed high inhibitory effects on Ulva attachment and growth. Thus N. yezoensis powder, culture filtrates, and thalli displayed strong inhibitory effects on U. prolifera gametes, suggesting N. yezoensis attachment to ropes could be used to control green tides at the source.

Modelling welfare estimates in discrete choice experiments for seaweed-based renewable energy.

We explore what researchers can gain or lose by using three widely used models for the analysis of discrete choice experiment data-the random parameter logit (RPL) with correlated parameters, the RPL with uncorrelated parameters and the hybrid choice model. Specifically, we analyze three data sets focused on measuring preferences to support a renewable energy programme to grow seaweed for biogas production. In spite of the fact that all three models can converge to very similar median WTP values, they cannot be used indistinguishably. Each model is based on different assumptions, which should be tested before their use. The fact that standard sample sizes usually applied in environmental valuation are generally unable to capture the outcome differences between the models cannot be used as a justification for their indistinct application.

Novel amylomacins from seaweed-associated Bacillus amyloliquefaciens as prospective antimicrobial leads attenuating resistant bacteria.

The rise in antibiotic-resistant bacterial strains prompting nosocomial infections drives the search for new bioactive substances of promising antibacterial properties. The surfaces of seaweeds are rich in heterotrophic bacteria with prospective antimicrobial substances. This study aimed to isolate antibacterial leads from a seaweed-associated bacterium. Heterotrophic Bacillus amyloliquefaciens MTCC 12716 associated with the seaweed Hypnea valentiae, was isolated and screened for antimicrobial properties against drug-resistant pathogens. The bacterial crude extract was purified and three novel amicoumacin-class of isocoumarin analogues, 11'-butyl acetate amicoumacin C (amylomacin A), 4'-hydroxy-11'-methoxyethyl carboxylate amicoumacin C (amylomacin B) and 11'-butyl amicoumacin C (amylomacin C) were isolated to homogeneity. The studied amylomacins possessed potential activities against Pseudomonas aeruginosa, vancomycin-resistant Enterococcus faecalis, Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, and Shigella flexneri with a range of minimum inhibitory concentration values from 0.78 to 3.12 µg/mL, although standard antibiotics ampicillin and chloramphenicol were active at 6.25-25 µg/mL. Noticeably, the amylomacin compound encompassing 4'-hydroxy-11'-methoxyethyl carboxylate amicoumacin C functionality (amylomacin B), displayed considerably greater antagonistic activities against methicillin-resistant S. aureus, vancomycin-resistant E. faecalis, Vibrio parahaemolyticus, Escherichia coli, and K. pneumoniae (minimum inhibitory concentration 0.78 µg/mL) compared to the positive controls and other amylomacin analogues. Antimicrobial properties of the amylomacins, coupled with the presence of polyketide synthase-I/non-ribosomal peptide synthetase hybrid gene attributed the bacterium as a promising source of antimicrobial compounds with pharmaceutical and biotechnological applications.

Role and Evolution of the Extracellular Matrix in the Acquisition of Complex Multicellularity in Eukaryotes: A Macroalgal Perspective.

Multicellular eukaryotes are characterized by an expanded extracellular matrix (ECM) with a diversified composition. The ECM is involved in determining tissue texture, screening cells from the outside medium, development, and innate immunity, all of which are essential features in the biology of multicellular eukaryotes. This review addresses the origin and evolution of the ECM, with a focus on multicellular marine algae. We show that in these lineages the expansion of extracellular matrix played a major role in the acquisition of complex multicellularity through its capacity to connect, position, shield, and defend the cells. Multiple innovations were necessary during these evolutionary processes, leading to striking convergences in the structures and functions of the ECMs of algae, animals, and plants.

Local conditions magnify coral loss after marine heatwaves.

Climate change threatens coral reefs by causing heat stress events that lead to widespread coral bleaching and mortality. Given the global nature of these mass coral mortality events, recent studies argue that mitigating climate change is the only path to conserve coral reefs. Using a global analysis of 223 sites, we show that local stressors act synergistically with climate change to kill corals. Local factors such as high abundance of macroalgae or urchins magnified coral loss in the year after bleaching. Notably, the combined effects of increasing heat stress and macroalgae intensified coral loss. Our results offer an optimistic premise that effective local management, alongside global efforts to mitigate climate change, can help coral reefs survive the Anthropocene.

Colonization of macroalgal deposits by estuarine nematodes through air and potential for rafting inside algal structures.

Dispersal is an important life-history trait. In marine meiofauna, and particularly in nematodes, dispersal is generally considered to be mainly passive, i.e. through transport with water currents and bedload transport. Because nematodes have no larval dispersal stage and have a poor swimming ability, their per capita dispersal capacity is expected to be limited. Nevertheless, many marine nematode genera and even species have near-cosmopolitan distributions, and at much smaller spatial scales, can rapidly colonise new habitat patches. Here we demonstrate that certain marine nematodes, like the morphospecies Litoditis marina, can live inside macroalgal structures such as receptacula and-to a lesser extent-floating bladders, which may allow them to raft over large distances with drifting macroalgae. We also demonstrate for the first time that these nematodes can colonize new habitat patches, such as newly deposited macroalgal wrack in the intertidal, not only through seawater but also through air. Our experimental set-up demonstrates that this aerial transport is probably the result of hitchhiking on vectors such as insects, which visit, and move between, the patches of deposited algae. Transport by wind, which has been observed for terrestrial nematodes and freshwater zooplankton, could not be demonstrated. These results can be important for our understanding of both large-scale geographic distribution patterns and of the small-scale colonization dynamics of habitat patches by marine nematodes.