Thymic stromal lymphopoietin induces IL-4/IL-13 from T cells to promote sebum secretion and adipose loss.

BACKGROUND: The cytokine TSLP promotes type 2 immune responses and can induce adipose loss by stimulating lipid loss from the skin through sebum secretion by sebaceous glands, which enhances the skin barrier. However, the mechanism by which TSLP upregulates sebaceous gland function is unknown. OBJECTIVES: This study investigated the mechanism by which TSLP stimulates sebum secretion and adipose loss. METHODS: RNA-sequencing analysis was performed on sebaceous glands isolated by laser capture microdissection and single-cell RNA-sequencing analysis was performed on sorted skin T cells. Sebocyte function was analyzed by histological analysis and sebum secretion in vivo and by measuring lipogenesis and proliferation in vitro. RESULTS: This study found that TSLP sequentially stimulated the expression of lipogenesis genes followed by cell death genes in sebaceous glands to induce holocrine secretion of sebum. TSLP did not affect sebaceous gland activity directly. Rather, single-cell RNA-sequencing revealed that TSLP recruited distinct T-cell clusters that produce IL-4 and IL-13, which were necessary for TSLP-induced adipose loss and sebum secretion. Moreover, IL-13 was sufficient to cause sebum secretion and adipose loss in vivo and to induce lipogenesis and proliferation of a human sebocyte cell line in vitro. CONCLUSIONS: This study proposes that TSLP stimulates T cells to deliver IL-4 and IL-13 to sebaceous glands, which enhances sebaceous gland function, turnover, and subsequent adipose loss.

Acne and diet: a review of pathogenic mechanisms.

Acne is a chronic inflammatory disease of the pilosebaceous unit with multifactorial etiology. Abnormal proliferation of keratinocytes, altered sebum production, inflammation of the sebaceous follicle, and colonization by Cutibacterium acnes have been traditionally implicated. However, the diet has also been highlighted in the pathogenesis because of its direct relation with some biochemical markers and the transcription of specific genes associated with sebaceous gland activity, inflammation, and bacterial proliferation, which together promote the development of the disease, affect the severity of the condition, and modify its response to treatment.

El acné es una enfermedad inflamatoria crónica de la unidad pilosebácea de etiología multifactorial, en la que clásicamente se han implicado la proliferación anormal de queratinocitos, la producción alterada de sebo, la inflamación del folículo sebáceo y la colonización por Cutibacterium acnes. Sin embargo, también destaca la dieta en la patogenia al relacionarse directamente con la alteración de algunos marcadores bioquímicos y transcripción de ciertos genes que se asocian con la actividad de la glándula sebácea, la inflamación y la proliferación bacteriana, que en conjunto promueven el desarrollo de la enfermedad, afectan la gravedad del cuadro y modifican su respuesta al tratamiento.

Modulation of skin androgenesis and sebum production by a dermocosmetic formulation.

BACKGROUND: Excessive androgenesis in the skin promotes sebaceous hyperproduction which is the onset of acne vulgaris pathogenesis. Free fatty acids and lipid accumulation in the glandular infundibulum culminates in microbiota imbalance, triggering inflammatory response and follicular hyperkeratinization. AIMS: The purpose of this work was to present an alternative cosmetic treatment for acne skin care, focusing on the prevention of sebaceous gland dysregulation. METHODS: Insulin-stimulated human sebocytes were treated with noncytotoxic concentrations of a DTRW cosmetic formulation. After 6 days of incubation, cell lysates were collected for testosterone, 5α-reductase, and dyhidrotestosterone (DHT) quantitation. In parallel, cells were stained with Oil Red O to measure sebum production. RESULTS: Human sebocytes were incubated with insulin to mimic a seborrheic microenvironment with overproduction of intracellular lipids and fatty acids. Concomitant incubation of cell cultures with DRTW was able to promote a 52.97% reduction in intracellular lipid content. The anti-androgenic properties of DRTW had been proved by the reductions of testosterone (↓59.90%), 5α reductase (↓59.34%), and DHT (↓55.98%) levels in sebocyte cultures also stimulated with insulin. CONCLUSION: The results indicate a promising action of DRTW cosmetic formulation in preventing the development of acne lesions by mechanisms involving the modulation of cutaneous androgenesis and consequently the control of sebum overproduction, considered one of the leading causes of acne.

Caracterización de la población con obstrucción de poros faciales a quienes se les aplico laserterapia en la Unidad de Dermatología UNIMEL / Characterization of the population with facial pore obstruction to whom laser therapy was applied at the UNIMEL Dermatology Unit / Caracterização da população com obstrução dos poros faciais a quem foi aplicada terapia a laser no Serviço de Dermatologia da UNIMEL

INTRODUCCIÓN: el poro es un orificio de excreción que se encuentra en la epidermis de la piel, en donde pueden producirse problemas de eliminación deficiente del sebo en personas que presentan exceso de grasas. OBJETIVO: analizar las características y tratamiento laser aplicado a la población con obstrucción de poros faciales que acuden a la consulta de la Unidad de Dermatología UNIMEL. MATERIALES Y MÉTODOS: estudio descriptivo, de campo, prospectivo y transversal, la población fue de 653 pacientes de ambos sexos que acudieron a la consulta de dermatología y recibieron laserterapia por presentar obstrucción de poros faciales. Se aplicó estadística descriptiva para la representación de datos. El instrumento utilizado fue una lista de registro. RESULTADOS: los pacientes presentaron una edad promedio de 37 años, se aplicaron diferentes tratamientos según los criterios diagnósticos, se les realizó el procedimiento de fotona Black peel en un 0,15%, Vela de Nova en un 5,05% y Qs Quimioespectra a un 94,79% que representan un total de 619 personas. CONCLUSIÓN: el mayor grupo de pacientes que asistió a tratamiento se ubicó entre los 31 a 40 años de edad, un alto porcentaje mujeres afectadas por secuelas de daño facial producido por obstrucción de los poros y acumulación de sebo, un grupo importante 48,8% presenta acné posterior a los 18 años de edad.

INTRODUCTION: the pore is an excretion hole found in the epidermis of the skin, where problems of poor sebum removal can occur in people with excess fat; irregular synthesis occurs in the production of sebum increases its production with occlusion of the pores by dead cells. OBJECTIVE: to analyze the characteristics and laser treatment applied to the population with facial pore obstruction that come to the UNIMEL Dermatology Unit consultation. METHODS: a descriptive, field, prospective and cross-sectional study, the study population was 653 patients of both sexes who attended the dermatology consultation and received laser therapy for presenting facial pore obstruction. Descriptive statistics were applied for data representation. The instrument used was a registration list. RESULTS: the patients had an average age of 37 years, different treatments were applied according to the diagnostic criteria, and the photone Black peel procedure was performed in 0.15%, Nova candle in 5.05% and Qs Chemospectra at 94.79% representing a total of 619 people. CONCLUSION: the largest group of patients who attended treatment was between 31 to 40 years of age, a high percentage of women affected by sequelae of facial damage caused by pore obstruction and sebum accumulation, an important group 48.8% you have acne after 18 years of age.

INTRODUÇÃO: o poro é um orifício de excreção que se encontra na epiderme da pele, onde podem ocorrer problemas com a má remoção de sebo em pessoas com excesso de gordura. OBJETIVO: analisar as características e o tratamento a laser aplicado à população com obstrução de poro facial que frequenta a consulta do Serviço de Dermatologia da UNIMEL MATERIAIS E MÉTODOS: estudo descritivo, de campo, prospectivo e transversal, a população foi de 653 pacientes de ambos os sexos que compareceu à consulta de dermatologia e recebeu terapia a laser devido à obstrução dos poros faciais. Estatísticas descritivas foram aplicadas para representar os dados. O instrumento utilizado foi uma lista de registro. RESULTADOS: os pacientes tinham idade média de 37 anos, diferentes tratamentos foram aplicados de acordo com os critérios diagnósticos, o procedimento de fotone Black peel foi realizado em 0,15%, Vela de Nova em 5,05% e Qs Chemospectra em 94,79% representando um total de 619 pessoas. CONCLUSÃO: o maior grupo de pacientes que frequentou o tratamento teve entre 31 e 40 anos de idade, elevado percentual de mulheres acometidas por sequelas de lesões faciais causadas por obstrução de poros e acúmulo de sebo, importante grupo 48,8% tem acne após 18 anos de idade.

Correlations between sebaceous glands activity and porphyrins in the oily skin and hair and immediate effects of dermocosmetic formulations.

BACKGROUND: Oily skin and hair not only contain a large amount of sebum, but also exhibit other changes that compromise their physiology. The immediate effects of dermocosmetics are very important for adhesion to treatment. AIM: The aim of the present study was to characterize oily skin and scalp, to evaluate the correlation of sebum production with porphyrin counts and the immediate effects of topical formulations for sebum control. PATIENTS/METHODS: A total of 100 women aged 18-49 years were recruited. Sebaceous gland activity, sebum amount, stratum corneum water content (SCWC) transepidermal water loss (TEWL), skin gloss, amount of porphyrins and pores were determined in the face and SCWC, sebum amount, porphyrin count, and TEWL were also determined in the scalp. The immediate effects of formulations containing a guarana extract were determined after 2 hours of application. RESULTS: A correlation between sebaceous gland activity and presence of porphyrins in the frontal region of the face was detected. Low gloss values and large amounts of pores in the malar region were related to lower skin uniformity. High sebum values and low SCWC and porphyrin count were also observed in the vertex region. The studied formulations reduced the sebum content of face and scalp after 2 hours of application. CONCLUSION: Oily skin and hair showed high sebum values, which were correlated with porphyrin count and with the activity of sebaceous glands. Finally, the studied formulations had immediate reducing effects on sebum amounts on the skin and scalp.

Adult female acne: a guide to clinical practice.

BACKGROUND: Acne in women is often associated with anxiety and depression, and may persist from adolescence as well as manifest for the first time in adulthood. Genetic and hormonal factors contribute to its etiopathogenesis, and maintenance treatment is required, usually for years, due to its clinical evolution. OBJECTIVE: To develop a guide for the clinical practice of adult female acne. METHODS: A team of five experts with extensive experience in acne conducted a literature review of the main scientific evidence and met to discuss the best practices and personal experiences to develop a guide containing recommendations for the clinical practice of adult female acne. RESULTS: The group of specialists reached consensus on the main guidelines for clinical practice, providing detailed recommendations on clinical picture, etiopathogenesis, laboratory investigation and treatment of adult female acne. CONCLUSION: Different from teenage acne, adult female acne presents some characteristics and multiple etiopathogenic factors that make its management more complex. This guide provides recommendations for best clinical practices and therapeutic decisions. However, the authors consider that additional studies are needed in order to provide more evidence for adult female acne to be better understood.

Adult female acne: a guide to clinical practice

Abstract: Background: Acne in women is often associated with anxiety and depression, and may persist from adolescence as well as manifest for the first time in adulthood. Genetic and hormonal factors contribute to its etiopathogenesis, and maintenance treatment is required, usually for years, due to its clinical evolution. Objective: To develop a guide for the clinical practice of adult female acne. Methods: A team of five experts with extensive experience in acne conducted a literature review of the main scientific evidence and met to discuss the best practices and personal experiences to develop a guide containing recommendations for the clinical practice of adult female acne. Results: The group of specialists reached consensus on the main guidelines for clinical practice, providing detailed recommendations on clinical picture, etiopathogenesis, laboratory investigation and treatment of adult female acne. Conclusion: Different from teenage acne, adult female acne presents some characteristics and multiple etiopathogenic factors that make its management more complex. This guide provides recommendations for best clinical practices and therapeutic decisions. However, the authors consider that additional studies are needed in order to provide more evidence for adult female acne to be better understood.

Molecular Interactions of Different Steroids Contributing to Sebum Production. Review.

Considerable progress has been made in learning about the physiology and biochemistry of the sebaceous glands and several of the diseases that affect this component of the skin. Of these diseases, acne has particular importance. It is associated with adolescence, and because of the hormonal changes that take place in this stage, when it is severe it can cause depression. Moreover, in a considerable proportion of acne sufferers both adolescent and adult, it can produce tumors and deformation of the sebaceous glands. This seriously affects the sufferers to the point where it may limit their professional activities because they do not want to be seen in public. Several important issues from classic studies on the sebaceous gland will be reviewed in this document to report the state of the art of current treatments for the pathology of these glands. The sebaceous gland is an intracrine organ, capable of synthesizing and metabolizing different steroidal hormones. The role of each of the enzymes involved in these processes of the skin will be analyzed. The presence of different hormone receptors in the scientific literature will be also reviewed, due to the role of the sebaceous gland in lipogenesis at different ages. We also describe the mechanism of action of androgens and progestins in relation to coregulators recruited for lipogenesis in this gland. We propose several new steroidal compounds based on their mechanism of action to block lipogenesis in the sebaceous glands. These molecules offer potential for new treatment options for skin diseases.

Unilateral acne after facial palsy.

We describe a case of unilateral acne which appeared after an episode of facial nerve palsy. An 18-year-old female patient presented with papules and pustules predominantly located on the side where the facial paralysis occurred. The patient suffered right facial paralysis, which was treated with prednisone and kinesiotherapy with massages, electrostimulation, and infrared light. Two weeks later, acne lesions appeared in the area affected by the paralysis. As suggested in other cases of paralysis, including cases of Parkinson's disease and spinal cord injury, an increased sebum excretion rate and the immobility of the affected area are most likely what caused the unilateral acne lesions.

Unilateral acne after facial palsy

Abstract: We describe a case of unilateral acne which appeared after an episode of facial nerve palsy. An 18-year-old female patient presented with papules and pustules predominantly located on the side where the facial paralysis occurred. The patient suffered right facial paralysis, which was treated with prednisone and kinesiotherapy with massages, electrostimulation, and infrared light. Two weeks later, acne lesions appeared in the area affected by the paralysis. As suggested in other cases of paralysis, including cases of Parkinson's disease and spinal cord injury, an increased sebum excretion rate and the immobility of the affected area are most likely what caused the unilateral acne lesions.

Characterization of oily mature skin by biophysical and skin imaging techniques.

BACKGROUND: The skin is a complex biological system and may suffer change according to the environmental factors, as higher temperatures can increase sebum excretion, presenting oiliness and acne. These alterations can persist during the aging and provoke more changes in aged skin. In this study we evaluated the mature oily skin characteristics using biophysical and skin imaging techniques. MATERIAL AND METHODS: Sixty healthy female subjects, aged between 39 and 55 years old were recruited and separated into 2 groups according to their skin type: normal/dry and oily skin. The skin was evaluated in terms of stratum corneum water content, transepidermal water loss (TEWL) sebum content, dermis thickness and echogenicity, skin microrelief, and pores content. RESULTS: The mature oily skin presented no significant differences when compared to the normal/dry skin on the stratum corneum water content and TEWL parameters. The sebum content was significantly higher on the oily skin group. The microrelief analysis showed an increase of skin roughness values in the oily skin and increase of scaliness in the normal/dry skin. The oily skin showed lower dermis echogenicity mainly in the frontal region and higher dermis thickness when compared to normal/dry skin. CONCLUSION: The mature oily skin showed different characteristics from normal/dry skin in terms of sebum content, microrelief parameters, and dermis thickness. This way, the characterization of mature oily skin in an objective way is very important to development of dermocosmetic products for more effective treatments focused specially on this type of skin.

Skin barrier and microbiome in acne.

Acne is an immune-mediated chronic inflammatory disease. Although several factors are involved in its pathophysiology, this process is not completely understood. Androgen hormone activity increases sebum production inside the pilosebaceous follicle, adjusting the environment for the development of Propionibacterium acnes which triggers inflammation. Knowing how others factors such as the skin barrier and microbiome are involved in acne, can help in understanding more about the disease and may help to conduct a better treatment.

Low-dose oral isotretinoin for moderate to severe seborrhea and seborrheic dermatitis: a randomized comparative trial.

BACKGROUND: The efficacy of low-dose oral isotretinoin in the treatment of seborrhea and seborrheic dermatitis has been poorly investigated in randomized studies. OBJECTIVES: This study was designed to determine the efficacy and safety of low-dose oral isotretinoin in the treatment of moderate to severe seborrhea and seborrheic dermatitis on the scalp and/or face. METHODS: A randomized, comparative clinical trial, using two groups, was conducted over 6 months. Patients in Group ISO were treated with isotretinoin 10 mg every other day. In Group X, patients received antiseborrheic topical treatment. Patient opinion, investigator assessment, scalp pruritus, sebum production, and quality of life (QoL) comprised the efficacy outcomes. RESULTS: The intention-to-treat population comprised a total of 45 patients with mean ± standard deviation ages of 28.7 ± 5.8 years in Group ISO and 29.8 ± 6.5 years in Group X. The rate of sebum production significantly decreased in Group ISO. Patient opinion, investigator, and QoL assessments improved in both groups. CONCLUSIONS: Low-dose oral isotretinoin can be a therapeutic modality for moderate to severe seborrhea and seborrheic dermatitis.

Skin barrier in rosacea.

Recent studies about the cutaneous barrier demonstrated consistent evidence that the stratum corneum is a metabolically active structure and also has adaptive functions, may play a regulatory role in the inflammatory response with activation of keratinocytes, angiogenesis and fibroplasia, whose intensity depends primarily on the intensity the stimulus. There are few studies investigating the abnormalities of the skin barrier in rosacea, but the existing data already show that there are changes resulting from inflammation, which can generate a vicious circle caused a prolongation of flare-ups and worsening of symptoms. This article aims to gather the most relevant literature data about the characteristics and effects of the state of the skin barrier in rosacea.

Skin barrier in rosacea

Abstract Recent studies about the cutaneous barrier demonstrated consistent evidence that the stratum corneum is a metabolically active structure and also has adaptive functions, may play a regulatory role in the inflammatory response with activation of keratinocytes, angiogenesis and fibroplasia, whose intensity depends primarily on the intensity the stimulus. There are few studies investigating the abnormalities of the skin barrier in rosacea, but the existing data already show that there are changes resulting from inflammation, which can generate a vicious circle caused a prolongation of flare-ups and worsening of symptoms. This article aims to gather the most relevant literature data about the characteristics and effects of the state of the skin barrier in rosacea.

Demodex folliculorum: its association with oily skin surface rather than rosacea lesions.

BACKGROUND: Despite the predilection of Demodex folliculorum (DF) for human sebum, observational studies addressing a relationship between this agent and rosacea paid no attention to the effect of a potentially acting confounder, the oily nature of the skin. OBJECTIVES: To analyze whether a relationship exists between the presence of the mite in rosacea and oily cutis. MATERIALS AND METHODS: An observational study on 63 rosacea cases and 61 healthy controls, both discriminated according to their predominance of oily or dry cutis, was carried out to investigate demodectic density by the skin surface biopsy. RESULTS: While comparisons on the density of DF revealed no differences between patients and controls (Mann-Whitney U-test, P = 0.35), persons with an oily cutis had increased amounts of this mite on the skin surface (Mann-Whitney U-test, P < 0.05), irrespective of whether they had rosacea or not. CONCLUSIONS: The association between DF and oily skin rather than rosacea lesions gives no support for this agent being implied in disease pathogenesis.

Clinical scoring and instrumental analysis to evaluate skin types.

BACKGROUND: The biology of the skin is very complex, and there are a number of methods used to classify the different skin types. It is possible to measure or quantify the characteristics of the specific skin types, using a variety of techniques that can objectively evaluate the properties of the skin in a noninvasive manner. AIM: To clinically characterize different skin types by dermatological evaluation and biophysical and skin imaging techniques, and to evaluate the relationship between the different characteristics. METHODS: The study recruited 26 volunteers. Clinical scoring was performed by a dermatologist who classified the volunteers' skin as normal or dry (group 1) and combination or oily (group 2). Objective measurements included skin microrelief, pH, oiliness, water content of the stratum corneum and transepidermal water loss (TEWL). RESULTS: Positive correlations were found between the level of skin oiliness and skin texture obtained from both instrumental analysis and clinical scoring. The combination and oily skin types had higher clinical scores for shine intensity, oiliness and tendency to pigmentation, and also had higher objective scores for sebum secretion, TEWL and roughness. CONCLUSIONS: Biophysical and skin imaging techniques are effective tools to help characterize skin type and assist in clinical dermatology. We found that different skin types had different characteristics related to skin microrelief, oiliness and TEWL, and therefore require specific dermatological treatments.

Effect of different levels of methionine, protein and tallow on the productive performance and egg quality of laying hens in the late-phase production

An experiment was conducted to determine the effects of different levels of methionine, protein and tallow on productive performance and egg quality of laying hens in the late phase of production. A completely randomized design with a 3×2×2 factorial arrangement, with three levels (0.34, 0.31, and 0.27%) of methionine (MET), two levels (12.8 and 14.7%) of protein (PRO) and two levels (1 and 3%) of tallow (TAL) with constant level of linoleic acid (1.55 ± 0.02%), was used. A number of 144 Hi-Line W-36 layers from 70 to 76 wk of age was randomly distributed into 12 treatment groups with 4 replicates of 3 hens each. Egg production and egg weight were daily recorded and feed intake and egg quality traits were recorded every 2 wk. There was a significant interaction between PRO levels and TAL for egg weight. Low levels of TAL and PRO decreased egg weight throughout the experiment. High levels of MET and TAL with concomitant reduced PRO, increased eggshell thickness, and a significant interaction between levels of MET, PRO and TAL was observed during the experiment (70 to 76 wk). Low level of protein (12.8%) significantly decreased albumen weight in the third 2-wk period. Yolk color increased when hens were fed low levels of PRO and TAL. Results of this experiment indicated that the simultaneous reduction of dietary PRO and MET in diets of Hi-Line W-36 laying hens in the late phase of production, reduced egg weight (P<0.05). Productive performance and egg quality were not affected by 12 and 20% reduction of PRO and MET, respectively. It seems that decreasing the levels of MET and PRO to lower than the recommended values can decrease egg weight without negative effects on productive performance and egg quality of laying hens in the late phase of production.(AU)

Estudo das mudanças quali e quantitativas da participação de ácidos graxos no sebo de pacientes com acne inflamatória, graus II ou III, em uso sistêmico de limeciclina e/ou suplementação oral à base de ácidos linoleico, gamalinolênico / Study of qualitative and quantitative changes of fatty acids in the sebum of patients with inflammatory acne, grade II or III, under systemic use of lymecycline and/or oral supplementation based on linoleic, gamma-linolenic, and oleic acids using gas

Introdução: Acne é uma dermatose que apresenta o padrão do sebo alterado, tanto quali, quanto quantitativamente. Objetivos: Estudar as possíveis alterações quali e quantitativas dos ácidos graxos encontrados no sebo de portadores de acne vulgar graus II ou III mediante ao uso diário de limeciclina e/ou suplemento oral a base de ácidos linoleico, gamalinolênico e oleico. Métodos: Quarenta e cinco sujeitos de pesquisa masculinos, de 12 a 40 anos de idade, portadores de acne vulgar graus II ou III, submeteram-se a 90 dias de uso de: Grupo A, 300mg/dia de limeciclina; Grupo B, 540mg de ácidos gamalinolênico, 1.200mg linoleico e 510mg oleico/dia; Grupo C: Grupos A+B. A cada 30 dias, eles tinham amostra de sebo da fronte coletada para análise cromatográfica dos ácidos graxos nele presentes. Resultados: Quarenta sujeitos de pesquisa (88,9 por cento) concluíram o estudo, os quais ingeriram todas as doses dos produtos, sem apresentarem eventos adversos em qualquer dos Grupos. O número de comedões, pústulas e cistos reduziu com o tempo (p<0,001, para todos), nos três Grupos. Com relação ao número total de lesões, houve uma maior quantidade de lesões em pacientes do Grupo B, quando comparados aos do Grupo A (p=0,033) e aos do Grupo C (p=0,030). Sete ácidos graxos apresentaram mudanças de seu padrão durante o estudo. De um modo geral, as mudanças que se tornaram mais evidentes foram: 1) a concentração de esqualeno no SB não reduziu em qualquer Grupo de estudo; 2) C12:0, C14:0 e C16:1 tiveram suas respectivas concentrações aumentadas nos três Grupos com o tempo de terapêuticas; 3) há indicativo de aumento de C18:1n9c+C18:1n9t na associação das duas classes terapêuticas estudadas; 4) limeciclina e/ou ingestão de ácido linoleico não aumentaram a participação do ácido linoleico no sebo; 5) ácido -linolênico teve sua concentração aumentada e, depois diminuída com o uso de LM e/ou com sua própria suplementação oral...

Introduction: Acne is a dermatosis that presents an altered sebum pattern, quantitatively and qualitatively. Objectives: To study the possible quantitative and qualitative changes of fatty acids found in the sebum of patients with acne vulgaris grade II or III, through the daily use of lymecycline and/or oral supplementation based on linoleic, gamma-linolenic, and oleic acids. Methods: Fortyfive male research subjects, ages 12 to 40, presenting acne vulgaris grade II or III, were submitted to a 90-day use of: Group 1, 300mg of lymecycline per day; Group 2, 540mg of gamma-linolenic acid, 1,200mg of linoleic acid, and e 510mg of oleic acid per day; Group 3: Groups A+B. Every 30 days, a sample of the sebum of their forehead was collected for chromatographic analysis of the fatty acids contained therein. Results: Forty research subjects (88.9 percent ) concluded the study. They ingested all dosages of the products, without presenting side effects in any of the Groups. The number of comedones, pustules, and cysts reduced with time (p<0.001, for all), in all the 3 Groups. With respect to the total number of lesions, Group B´s patients presented a larger quantity of lesions, as compared to those of Group A (p=0.033) and Group C (p=0.030). Seven fatty acids presented pattern changes during the study. In general, the changes that became more evident were: 1) the squalene concentration in the sebum did not diminish in any Group under study; 2) C12:0, C14:0, and C16:1 had their respective concentrations increased in the 3 Groups over the time of the treatments; 3) there is an indication for increasing of C18:1n9t+C18:1n9c when both of therapies are associated; 4) lymecycline and/or the ingestion of linoleic acid did not increase the participation of linoleic acid in the sebum; and 5) gama-linolenic acid had its concentration increased during the first 60-day and diminished during the last 30-day therapies period using lymecycline and/or with its own oral supplementation...

Experiencia clínica de un tratamiento a base de vitamina C glicosilada, ácido salicílico y zincadone ATM en hombres chilenos con piel mixta a grasa hasta acné inflamatorio leve / Action and tolerance of a formulation with glycosylated vitamin C, zincadone ATM and salicylic acid on Chilean male patients with greasy or mixed normal/greasy skin and/or mild inflammatory acne

Con el fin de evaluar la acción y tolerancia de una formulación a base de vitamina CG, Zincadone ATM y ácido salicílico se reclutaron 65 hombres con piel facial mixta, seborreica y/o con acné inflamatorio leve. Durante un período de 60 días se realizó un estudio clínico abierto y prospectivo en el cual se cuantificó clínicamente el número de lesiones no inflamatorias e inflamatorias, se evaluó el grado de dilatación de los poros a través de escala visual y con lente 30X, se cuantificó la presencia de sebo mediante sebumetría y el porcentaje de hidratación de la piel del rostro. Adicionalmente se utilizó el sistema VISIA para realizar un análisis de la dilatación de los poros, porfirinas cutáneas y textura de la piel. Se observó una mejoría significativa en cada uno de los parámetros medidos.

An open and prospective study was performed in order to evaluate the action and tolerance of a formulation with Glycosylated Vitamin C, Zincadone ATM and Salicylic Acid on 65 male patients with greasy or mixed normal/greasy skin and/or mild inflammatory acne during a period of sixty days. The degree of dilated pores was evaluated both visually and through a 30X lens. The number of inflammatory and non inflammatory lesions, sebumetry and moisture percentage were clinically quantified. Additionally, the VISIA system was utilized to evaluate dilated pores, cutaneous porphyrines and skin texture. There was a significant improvement in each one of the parameters studied.