ABSTRACT
A definitive diagnosis of tuberculosis serositis (TS) is still challenging. Our preliminary practice found that Serous Fluid Drainage Flocky Precipitate (SFDFP) was a useful testing sample to diagnose TS. We designed this study to assess the diagnostic performance of SFDPF for TS compared with conventional bacteriology methods on serous fluid (SF). A cohort study was conducted from July 2014 to April 2016. Patients with suspected TS were consecutively screened. SF and SFDFP were collected and tested by Ziehl-Neelsen stain, MTB culture, and Xpert/RIF assay. We compared the diagnostic performance of SF and SFDFP in several test settings. Through this study, 85 patients were enrolled, of whom 70 (82.4%) were confirmed TS or highly probable TS, 13 (15.3%) were none-TS and 2 (2.4%) indeterminate results were ruled out. The overall sensitivity using both SFDFP and SF was significantly higher than each (60% vs. 48% and 41%, p < 0.05). SFDFP and SF samples had similar diagnostic performance (p < 0.05). No false positive was detected in this study. We concluded that SFDFP is a reliable testing sample for diagnosing tuberculous serositis. SFDFP may significantly improve the diagnostic yield as a supplement to conventional tests.
Subject(s)
Molecular Diagnostic Techniques/methods , Serositis/metabolism , Tuberculosis, Cardiovascular/diagnosis , Adolescent , Adult , Aged , Aged, 80 and over , Body Fluids/diagnostic imaging , Body Fluids/metabolism , Child , Child, Preschool , China , Cohort Studies , Female , Humans , Male , Middle Aged , Mycobacterium tuberculosis/genetics , Sensitivity and Specificity , Serositis/diagnosis , Serositis/microbiology , Specimen Handling/methods , Sputum/microbiology , Tuberculosis, Meningeal/diagnosisABSTRACT
The Yinzhihuang injection, a traditional Chinese medicine, has been the recent target of increasing interest due to its anti-inflammatory properties. The molecular basis by which Yinzhihuang injection could cure Riemerella anatipestifer (RA) serositis in ducks is unclear. This study evaluated the antibacterial, anti-inflammatory and antioxidant effects of Yinzhihuang injection, using disease models of RA-induced infectious serositis in ducks and heptane-induced inflammation in mice and rats. The duck mortality rate was reduced from 60% to 20% and both the inflammatory response and histological damage were ameliorated by treatment with Yinzhihuang injection (0.02 g/kg). Further studies indicated that superoxide dismutase (SOD), nitric oxide synthase (NOS), and inducible nitric oxide synthase (iNOS) were elevated while malondialdehyde (MDA), nitric oxide (NO) and RA growth were inhibited when the ducks were treated by Yinzhihuang injection. In addition, Yinzhihuang injection (0.04 g/ml) effectively inhibited xylene-induced auricle swelling in mice, (demonstrating an inhibition rate of 35.21%), egg albumen-induced paw metatarsus swelling in rats, (demonstrating an inhibition rate of 22.30%), and agar-induced formation of granulation tissue. These results suggest that Yinzhihuang injection ameliorates RA-induced infectious serositis in ducks by modulation of inflammatory mediators and antioxidation.
Subject(s)
Anti-Bacterial Agents/therapeutic use , Anti-Inflammatory Agents/therapeutic use , Antioxidants/therapeutic use , Drugs, Chinese Herbal/therapeutic use , Ducks/microbiology , Flavobacteriaceae Infections/veterinary , Riemerella/drug effects , Serositis/veterinary , Animals , Anti-Bacterial Agents/administration & dosage , Anti-Inflammatory Agents/administration & dosage , Antioxidants/administration & dosage , Drugs, Chinese Herbal/administration & dosage , Ducks/metabolism , Flavobacteriaceae Infections/drug therapy , Flavobacteriaceae Infections/metabolism , Flavobacteriaceae Infections/microbiology , Injections , Male , Malondialdehyde/metabolism , Mice , Mice, Inbred BALB C , Nitric Oxide Synthase/metabolism , Poultry Diseases/drug therapy , Poultry Diseases/metabolism , Poultry Diseases/microbiology , Rats , Rats, Wistar , Serositis/drug therapy , Serositis/metabolism , Serositis/microbiology , Superoxide Dismutase/metabolismABSTRACT
No disponible
Subject(s)
Female , Humans , Male , Familial Mediterranean Fever/genetics , Familial Mediterranean Fever/metabolism , Serositis/congenital , Serositis/complications , Genetics/instrumentation , Genetics , Genetics/education , Genetics/ethics , Cryopyrin-Associated Periodic Syndromes/genetics , Cryopyrin-Associated Periodic Syndromes/metabolism , Familial Mediterranean Fever/complications , Familial Mediterranean Fever/pathology , Serositis/genetics , Serositis/metabolism , Genetics/classification , Cryopyrin-Associated Periodic Syndromes/complications , Cryopyrin-Associated Periodic Syndromes/diagnosisABSTRACT
Systemic lupus erythematosus (SLE) is an autoimmune disease, involving multiple organs. Diverse manifestations may obscure the diagnosis and confuse our thinking process, especially when few clues are present at the beginning. Serositis is one of the various presentations, and the presence of lupus erythematosus (LE) cell in body fluid may be a hint for the final diagnosis of SLE. Herein, we present a young female patient diagnosed of SLE with initial presentation of lupus peritonitis. Finding of LE cell in ascites prompted us for immunologic survey. Diagnosis of SLE was confirmed with high titer of anti-nuclear antibody and antibody to double-stranded DNA. Cytologic examination of body fluid is mainly useful in detecting malignant cells, but high specificity of this marker aids in early diagnosis of SLE.
Subject(s)
Ascites/metabolism , Cytological Techniques , Lupus Erythematosus, Systemic/diagnosis , Serositis/diagnosis , Adult , Autoimmune Diseases/diagnosis , Autoimmune Diseases/pathology , Cells, Cultured , Diagnosis, Differential , Female , Humans , Immune System , Lupus Erythematosus, Systemic/metabolism , Serositis/metabolismABSTRACT
Mesothelial cells (MC) form a polarized monolayer lining serosal cavities. During serositis, the MC lining undergoes hyperplasia, and MC are shed into effusions. During these processes, contact with basement membrane and, ultimately, neighboring cells is at least temporarily lost, suggesting regulated alterations in cell/matrix and cell/cell adhesion. Such interactions are primarily mediated by integrins. Malignant mesothelioma has a growth pattern characterized by lateral, limited invasive but contiguous spread. During serositis, activated MC, both sessile and detached, expressed an extended spectrum of beta1, beta3 and beta4 integrins compared with resting MC, as shown by immunohistology. Malignant mesothelioma had an integrin repertoire and a subcellular distribution resembling that of activated sessile rather than floating MC. In vitro, MC exposed a more comprehensive pattern of integrins than that of the newly established mesothelioma cell lines ME-HD-1 and ME-HD-2, as shown by flow cytometry. MC consistently adhered better than mesothelioma cells to laminin, tenascin, fibronectin and collagen type IV. Adhesion of MC and mesothelioma cells to these matrix proteins was, at least in part, mediated via beta1 integrins. The different integrin profiles and adhesion properties of cultured MC and mesothelioma cells may reflect a limited functional differentiation of the latter.
