Geography as non-genetic modulation factor of chicken cecal microbiota.

The gastrointestinal tract of chickens harbors a highly diverse microbiota contributing not only to nutrition, but also to the physiological development of the gastrointestinal tract. Microbiota composition depends on many factors such as the portion of the intestine as well as the diet, age, genotype, or geographical origin of birds. The aim of the present study was to demonstrate the influence of the geographical location over the cecal microbiota from broilers. We used metabarcoding sequencing datasets of the 16S rRNA gene publicly available to compare the composition of the Argentine microbiota against the microbiota of broilers from another seven countries (Germany, Australia, Croatia, Slovenia, United States of America, Hungary, and Malaysia). Geographical location played a dominant role in shaping chicken gut microbiota (Adonis R2 = 0.6325, P = 0.001; Mantel statistic r = 0.1524, P = 4e-04) over any other evaluated factor. The geographical origin particularly affected the relative abundance of the families Bacteroidaceae, Lactobacillaceae, Lachnospiraceae, Ruminococcaceae, and Clostridiaceae. Because of the evident divergence of microbiota among countries we coined the term "local microbiota" as convergent feature that conflates non-genetic factors, in the perspective of human-environmental geography. Local microbiota should be taken into consideration as a native overall threshold value for further appraisals when testing the production performance and performing correlation analysis of gut microbiota modulation against different kind of diet and/or management approaches. In this regard, we described the Argentine poultry cecal microbiota by means of samples both from experimental trials and commercial farms. Likewise, we were able to identify a core microbiota composed of 65 operational taxonomic units assigned to seven phyla and 38 families, with the four most abundant taxa belonging to Bacteroides genus, Rikenellaceae family, Clostridiales order, and Ruminococcaceae family.

Management practices that influence the welfare of calves on small family farms.

In this Research Reflection we review management practices in small family farms with less than 100 cows. Small farms represent the majority of farms in the EU and the world, and they are of great importance for the economy of a country. On cattle farms, the welfare of calves is of primary importance for the profitability of the herd, and poor management is one of the main factors influencing calf health and survival. Data on the risk factors for calf welfare issues in small-scale farms are limited. For this purpose, the literature data from six world countries were presented and compared, including Serbia and Slovenia where a survey related to the issue was carried out within the COST Action FA1308, DairyCare. Some practices within the following areas in calf management were considered: calving management, care for new-born calves, use of painful procedures, colostrum management, cow-calf separation, calf feeding, weaning, calf housing, and general monitoring. In each of the countries, the health and welfare of calves are threatened by some omissions in rearing practices and the major are related to the new-born calf management, the feeding and watering management, and the application of hygienic measures. Many farmers are well aware of the importance of proper calf rearing; others would need more incentive to improve calf management. Each country should pay attention to the education of farmers about the most common deficiencies in calf management.

Subtype distribution of zoonotic pathogen Cryptosporidium felis in humans and animals in several countries.

Cryptosporidium felis is the major etiologic agent of cryptosporidiosis in felines and has been reported in numerous human cryptosporidiosis cases. Sequence analysis of the 60-kDa glycoprotein (gp60) gene has been developed for subtyping C. felis recently. In this study, 66 C. felis isolates from the United States, Jamaica, Peru, Portugal, Slovakia, Nigeria, Ethiopia, Kenya, China, India and Australia were subtyped using the newly established tool. Forty-four specimens yielded gp60 sequences, generating 23 subtypes clustered in 4 subtype families (XIXa, XIXc, XIXd and XIXe) with high bootstrap support in a phylogenetic analysis of sequence data. Among them, XIXa showed high genetic diversity at the nucleotide level, with the formation of 18 subtypes from both cats and humans with different geographic distribution. In contrast, all 11 XIXd isolates derived from humans from various countries had identical sequences. Results of this study improve our understanding of the genetic diversity, host specificity and transmission dynamics of C. felis.

The Science of Salt: A global review on changes in sodium levels in foods.

This review aims to summarize and synthesize studies reporting on changes in sodium levels in packaged food products, restaurant foods, and hospital or school meals, as a result of salt reduction interventions. Studies were extracted from those published in the Science of Salt Weekly between June 2013 and February 2018. Twenty-four studies were identified: 17 assessed the changes in packaged foods, four in restaurant foods, two in hospital or school meals, and one in both packaged and restaurant foods. Three types of interventions were evaluated as part of the studies: voluntary reductions (including targets), labeling, and interventions in institutional settings. Decreases in sodium were observed in all studies (n = 8) that included the same packaged foods matched at two time points, and in the studies carried out in hospitals and schools. However, there was little to no change in mean sodium levels in restaurant foods. The pooled analysis of change in sodium levels in packaged foods showed a decrease in sodium in unmatched food products (-36 mg/100 g, 95% CI -51 to -20 mg/100 g) and in five food categories-breakfast cereals, breads, processed meats, crisps and snacks, and soups. Twenty-two of the 24 studies were from high-income countries, limiting the applicability of the findings to lower resource settings.

Ten-Item Internet Gaming Disorder Test (IGDT-10): Measurement invariance and cross-cultural validation across seven language-based samples.

The Ten-Item Internet Gaming Disorder Test (IGDT-10) is a short screening instrument developed to assess Internet gaming disorder (IGD) as proposed in the Diagnostic and Statistical Manual of MentalDisorders, fifth edition (DSM-5), adopting a concise, clear, and consistent item-wording. According to initial studies conducted in 2014, the instrument showed promising psychometric characteristics. The present study tested the psychometric properties, including language and gender invariance, in a large international sample of online gamers. In this study, data were collected from 7,193 participants comprising Hungarian (n = 3,924), Iranian (n = 791), English-speaking (n = 754), French-speaking (n = 421), Norwegian (n = 195), Czech (n = 496), and Peruvian (n = 612) online gamers via gaming-related websites and gaming-related social-networking-site groups. A unidimensional factor structure provided a good fit to the data in all language-based samples. In addition, results indicated both language and gender invariance on the level of scalar invariance. Criterion and construct validity of the IGDT-10 was supported by its strong association with the Problematic Online Gaming Questionnaire and moderate association with weekly gaming time, psychopathological symptoms, and impulsivity. The proportions of each sample that met the cut-off score on the IGDT-10 varied between 1.61% and 4.48% in the individual samples, except for the Peruvian sample (13.44%). The IGDT-10 shows robust psychometric properties and appears suitable for conducting cross-cultural and gender comparisons across seven languages. (PsycINFO Database Record (c) 2019 APA, all rights reserved).

Somatotype profile of a special police unit / Perfil de somatotipo de una unidad especial de policía

The purpose of this research was to investigate the somatotype characteristics police special units to give us a better idea about requirements of body composition and somatotype for training or/and selection process of new candidates. Therefore, an anthropometric analysis was conducted on 17 male members of Slovenian special police unit with mean age 31.12 ± 5.61 years, body height 179.46 ± 5.36 cm and body weight 79.84 ± 6.16 kg. They were assessed with anthropometric variables in accordance with ISAK. The following measurements were taken: upper arm circumference, flexed upper arm circumference, forearm circumference, thigh circumference, mid-thigh circumference, calf circumference, shoulder width, pelvic width, elbow diameter, wrist diameter, knee diameter, ankle diameter, sub scapular skin fold, triceps skin fold, biceps skin fold, forearm skin fold, abdomen skin fold, chest skin fold, supraspinale skin fold, thigh skin fold and calf skin fold. Also, the bioelectrical impedance TANITA TBF-105 was used for measurements of body weight, body mass index, lean body mass, the percentage of fat mass, absolute fat mass and the percentage of body water were measured. Somatotype components and the position in the somatoplot was calculated with the Somatotype 1.2.5. software. The values found in this study indicate a significant importance of mesomorph component for police special units and their nature of work with dominance of balanced mesomorph somatotype (2.59-6.49-1.98).

El propósito de este estudio fue investigar las características especiales del somatotipo de unidades especiales de la policía para otorgar una mejor idea acerca de los requisitos de la composición corporal y el somatotipo para el entrenamiento y el proceso de selección de los nuevos candidatos. Por lo tanto, se realizó un análisis antropométrico en 17 miembros masculinos de la unidad especial de la policía Eslovena con una edad media de 31,12±5,61 años, altura corporal 179,46±5,36 cm y peso corporal 79,84±6,16 kg. Fueron evaluados con variables antropométricas de acuerdo con ISAK. Se tomaron las siguientes medidas: circunferencia del brazo, circunferencia del brazo flexionado, circunferencia del muslo, circunferencia media del muslo, circunferencia de la pantorrilla, ancho del hombro, ancho de la pelvis, diámetro del codo, diámetro de la muñeca, diámetro de la rodilla, diámetro del tobillo, doblez de la piel subescapular, doblez de la piel del tríceps, doblez de la piel del bíceps, doblez de la piel del antebrazo, doblez de la piel del abdomen, doblez de la piel del tórax, doblez de la piel supraespinal, doblez de la piel del muslo y doblez de la piel de la pantorrilla. Además, la impedancia bioeléctrica TANITA TBF-105 se utilizó para realizar las siguientes mediciones: peso corporal, índice de masa corporal, masa corporal magra, porcentaje de masa grasa, masa grasa absoluta y el porcentaje de agua corporal. Los componentes del somatotipo y la posición en el somatoplot se calcularon con el Software Somatotype 1.2.5. Los valores encontrados en este estudio indican una importancia significativa del componente mesomorfo para las unidades especiales de la policía y su naturaleza de trabajo con el dominio del somatotipo mesomorfo balanceado (2,59-6,49-1,98).

Detection of novel Betapapillomaviruses and Gammapapillomaviruses in eyebrow hair follicles using a single-tube 'hanging droplet' PCR assay with modified pan-PV CODEHOP primers.

A modified pan-PV consensus-degenerate hybrid oligonucleotide primer (CODEHOP) PCR was developed for generic and sensitive detection of a broad-spectrum of human papillomaviruses (HPVs) infecting the cutaneous epithelium. To test the analytical sensitivity of the assay we examined 149 eyebrow hair follicle specimens from immunocompetent male patients. HPV DNA was detected in 60 % (89/149) of analysed eyebrow samples with a total of 48 different HPV sequences, representing 21 previously described HPVs and 27 putative novel HPV types. Evidence for ten novel HPV subtypes and seven viral variants, clustering to three out of five genera containing cutaneous HPVs, was also obtained. Thus, we have shown that the modified pan-PV CODEHOP PCR assay is able to identify multiple HPV types, even from different genera, in the same clinical sample. Overall, these results demonstrate that the pan-PV CODEHOP PCR is an excellent tool for screening and identification of novel cutaneous HPVs, even in samples with low viral loads.

Health Care System for Children and Adolescents in Slovenia.

Slovenia's health system is financed by a Bismarckian type of social insurance system with a single insurer for a statutory health insurance, which is fully regulated by national legislation and administered by the Health Insurance Institute of Slovenia. The health insurance system is mandatory, providing almost universal coverage (98.5% of the population). Children and adolescents have the right to compulsory health insurance as family members of an insured person until the end of their regular education. Slovenia has a lower number of physicians per capita than both the European Union and the Central and Eastern Europe countries. Slovenia is facing a workforce crisis, as the number of health professionals retiring is not adequately being replaced by new trainees. There is also a net deficit of nurses with university and college degrees. Physicians working with children and adolescents in primary level have a 5-year specialization in pediatrics. Slovenia tends to be in line with the goals for the development of pediatric health care on a primary level in European countries, which are to maintain the achieved level of quality, better and equitable access, and delivery of services, aiming to reduce inequalities in health of children and adolescents and provide for every child and adolescent in the best way possible.

Some indicators of fatness and motor fitness in Slovenian and Serbian children / Algunos indicadores de obesidad y aptitud motriz en niños eslovenos y serbios

The purpose of this study was to identify sex and age specific differences in fatness and motor fitness levels of children in two western Balkan countries in south-eastern Europe: Slovenia and Serbia. The sample consisted of 1,659 subjects of both sexes, aged from 9 to 15 years. Body height, body mass, triceps skinfold thickness, bent arm hang and standing long jump were used in the analysis. Ordinal regression was used to test differences in body mass index (BMI) categories among the countries. MANOVA was used to test the differences in motor fitness level. There were statistically significant (p<0.001) differences between the countries for the entire set of tested variables. Boys and girls from both countries had similar body height, body mass and BMI, yet Slovenians had smaller triceps skinfold thickness. Children from both countries were superior in standing long jump compared to their counterparts elsewhere in Europe. However, Slovenian boys and girls performed better than Serbian ones in both performed motor tests. Differences between countries in motor tests results were larger at age 9 than at age 14. Apparent trend was detected: with similar morphological characteristics, Slovenian children, especially girls, are physically fitter than Serbian ones. Sex is the only significant parameter (p<0.001) of classifying into a BMI category. Boys have an approximate odds ratio of 1.75 for passing into a higher BMI category as girls. Among girls, the differences between countries were greater since the prevalence of overweight and obese girls in Slovenia decreases with age, while the reverse trend is observed in Serbia.

El propósito de este estudio fue identificar las diferencias específicas de sexo y edad en la aptitud y niveles de condición física en niños de dos países de los Balcanes occidentales en el sudeste de Europa: Eslovenia y Serbia. La muestra consistió en 1.659 sujetos de ambos sexos, con edades entre los 9 y 15 años. Para el análisis se utilizaron la altura corporal, masa corporal, espesor del pliegue cutáneo del tríceps, brazo doblado al colgar y de pie en salto largo. Se utilizó la regresión ordinal para probar las diferencias en el índice de masa corporal (IMC) categorías entre los países. El análisis MANOVA fue utilizado para probar las diferencias en el nivel de condición motriz física. El conjunto de variables analizadas (p<0,001) entre los países no fueron estadísticamente significativas. Los niños y niñas de ambos países tenían similar altura y masa corporal e IMC, pero en los eslovenos los pliegues cutáneos del triceps más pequeños. Los niños de ambos países fueron superiores en salto largo en comparación con sus homólogos de otras partes de Europa. Sin embargo, los niños y las niñas eslovenos obtuvieron mejores resultados que los serbios en ambas pruebas de motricidad. Las diferencias en los resultados de las pruebas de motricidad entre los países fueron mayores a los 9 años de edad, a los 14 años se detectó una tendencia aparente: con características morfológicas similares, los niños eslovenos, particularmente las niñas, se encontraban en mejores condiciones físicas que los niños serbios. El sexo es el único parámetro significativo (p<0,001) de clasificar en una categoría de IMC. Los niños tienen una probabilidad promedio aproximada de 1,75 para pasar a una categoría superior IMC tal como las niñas. Entre las niñas, las diferencias entre los países eran mayores, ya que la prevalencia de las niñas con sobrepeso y obesidad en Eslovenia disminuyen con la edad, mientras que se observó una tendencia inversa en Serbia.

Cadmium, mercury and lead in the blood of urban women in Croatia, the Czech Republic, Poland, Slovakia, Slovenia, Sweden, China, Ecuador and Morocco.

OBJECTIVES: The aim of the study was to make an international comparison of blood levels of cadmium (B-Cd), lead (B-Pb) and mercury (B-Hg) of women in seven European, and three non-European cities, and to identify determinants. MATERIALS AND METHODS: About 50 women (age: 46-62) from each city were recruited (totally 480) in 2006-2009. Interview and questionnaire data were obtained. Blood samples were analysed in one laboratory to avoid interlaboratory variation. RESULTS: Between the European cities, the B-Pb and B-Cd results vary little (range of geometric means: 13.5-27.0 µg/l and 0.25-0.65 µg/l, respectively); the variation of B-Hg was larger (0.40-1.38 µg/l). Between the non-European cities the results for B-Pb, B-Cd and B-Hg were 19.2-68.0, 0.39-0.99 and 1.01-2.73 µg/l, respectively. Smoking was a statistically significant determinant for B-Cd, while fish and shellfish intakes contributed to B-Hg and B-Pb, amalgam fillings also contributed to B-Hg. CONCLUSIONS: The present results confirm the previous results from children; the exposure to lead and cadmium varies only little between different European cities suggesting that other factors than the living area are more important. The study also confirms the previous findings of higher cadmium and lead levels in some non-European cities. The geographical variation for mercury is significant.

Spiritual and religious beliefs as risk factors for the onset of major depression: an international cohort study.

BACKGROUND: Several studies have reported weak associations between religious or spiritual belief and psychological health. However, most have been cross-sectional surveys in the U.S.A., limiting inference about generalizability. An international longitudinal study of incidence of major depression gave us the opportunity to investigate this relationship further. METHOD: Data were collected in a prospective cohort study of adult general practice attendees across seven countries. Participants were followed at 6 and 12 months. Spiritual and religious beliefs were assessed using a standardized questionnaire, and DSM-IV diagnosis of major depression was made using the Composite International Diagnostic Interview (CIDI). Logistic regression was used to estimate incidence rates and odds ratios (ORs), after multiple imputation of missing data. RESULTS: The analyses included 8318 attendees. Of participants reporting a spiritual understanding of life at baseline, 10.5% had an episode of depression in the following year compared to 10.3% of religious participants and 7.0% of the secular group (p<0.001). However, the findings varied significantly across countries, with the difference being significant only in the U.K., where spiritual participants were nearly three times more likely to experience an episode of depression than the secular group [OR 2.73, 95% confidence interval (CI) 1.59­4.68]. The strength of belief also had an effect, with participants with strong belief having twice the risk of participants with weak belief. There was no evidence of religion acting as a buffer to prevent depression after a serious life event. CONCLUSIONS: These results do not support the notion that religious and spiritual life views enhance psychological well-being.

Knowledge about and acceptance of genetically modified organisms among pre-service teachers: a comparative study of turkey and Slovenia

Genetically modified organisms cannot be regarded as merely a topic for academic debate, since these have serious implications as a research field and for production based on genetic engineering. Public debates rarely base their arguments on elements rooted in scientific arguments and knowledge but are heavily loaded with emotions, opinions and informal reasoning. This study aimed to investigate the knowledge and acceptance of genetically modified organisms among prospective teachers in Slovenia and Turkey. Knowledge of genetic modification was measured with a two-tier instrument. The level of acceptance of genetic modification was measured with a 17-item instrument. Findings revealed that knowledge of genetics and biotechnology barely influenced the acceptability of genetic modification, and correlations are low. The relationship between knowledge and acceptance was not significant among Slovenian students and while significant for the Turkish or combined groups, the r values were only 0.179 and 0.244. It was found that differences in the acceptability of clusters of different kinds of genetically modified organisms do exist between the two countries. In both countries, participants recognized microorganisms and plants that produce something useful as the most acceptable organisms, while at the other end were animals used for consumption or as donors of organs. Practical implications for teaching are discussed and implications for further studies are drawn.

Lack of association of immune-response-gene polymorphisms with susceptibility to sarcoidosis in Slovenian patients.

Sarcoidosis is a chronic inflammatory disease, characterized by granulomatous inflammation, prominently involving the respiratory system. The etiology of this disease has not yet been elucidated and the contribution of genetic is not yet completely understood. We searched for novel candidate genes, utilizing a system biology approach, based on data from published transcriptional, proteomic and linkage studies of sarcoidosis. The search revealed several new potential candidate genes involved in the pathogenesis of inflammatory lung diseases: 25-(OH)-vitamin D(3)-1alpha-hydroxylase (CYP27B1), endothelin-1 (EDN1) and glutathione S-transferase Pi (GSTP1). Variants of selected polymorphisms: -1260/ C>A in CYP27B1, Lys198Asn in EDN1, and Ile105Val in GSTP1, were examined to determine if they confer susceptibility to sarcoidosis, based on an analysis of 180 Slovenian patients in comparison with 283 healthy controls. Polymerase chain reactions using allele-specific oligonucleotides were performed. This disease was not significantly associated with genotypes CC at -1260/ C>A polymorphism in CYP27B1 (P = 0.68, odds ratio (OR) = 1.10, 95% confidence interval (CI) = 0.75-1.61), GG genotype at Lys198Asn polymorphism in EDN1 (P = 1.00, OR = 0.97, 95%CI = 0.65-1.44) and AA genotypes at Ile105Val polymorphism in GSTP1 (P = 0.53, OR = 0.87, 95%CI = 0.60-1.27). There was no association of polymorphisms in any of the genes with sarcoidosis.

Critical factors influencing the establishment, maintenance and sustainability of population-based cancer control programs.

Developing and maintaining a comprehensive cancer control program are two distinct entities. Key issues related to building and sustaining cancer control programs include how to integrate initiatives and efforts across multiple constituencies addressing components of the implementation of cancer control and non-communicable disease programs, the processes used in different resource settings to achieve effective drug budgeting, health technology assessment and health economics, and how countries can support public and societal engagement. There are promising examples in both resource-rich and resource-challenged countries of constituencies that have developed programs which can contribute to comprehensive cancer control. Some take advantage of newer technology and information services, while others are more people and patient focused. Critical issues and factors for establishing and maintaining population-based comprehensive cancer control programs are identified and reviewed.

The relationship among knowledge of, attitudes toward and acceptance of genetically modified organisms (GMOs) among Slovenian teachers / La relación entre el conocimiento y actitudes en la aceptación sobre organismos genéticamente modificados (OGM) entre profesores eslovenos

The objective of this study was to investigate knowledge about, opinions on and attitudes toward and finally readiness to accept genetically modified organisms (GMO) among Slovenian teachers. On average, they have higher levels of knowledge in classical genetics, and poor levels of knowledge about modern issues in biotechnology, and their attitudes toward GMOs are not extreme. They make decisions based on the acceptability of a particular GMO and not on GMOs in general, following two patterns: genetic modifications (GM) microorganisms and plants are more acceptable than animals, and GMOs are more acceptable if they can not be used directly for consumption and produce something recognized as useful. The relationship among knowledge of, attitudes towards and readiness to accept GMO showed that there is no correlation between knowledge and attitudes, only a weak correlation between knowledge and acceptance, and a solid correlation between attitudes and readiness to accept GMO. The practical implication of our findings is that acceptance of GMOs will not be changed by providing new technical or scientific information to teachers but by changing attitudes. The appropriate strategies and actions for improving university courses in biotechnology and the implication for classroom science activities and future research are discussed.

Cutaneous larva migrans in two Slovenian travelers returning from Brazil.

Cutaneous larva migrans is a frequent phenomenon endemic in tropical and subtropical countries. In Europe only sporadic cases are reported. We present two cases of cutaneous larva migrans in two Slovenian tourists returning from a vacation in Brazil.

Acceptance of killing and homicide rates in nineteen nations.

BACKGROUND: International variation in homicide rates may be attributable to cultural differences in acceptance of moral justifications for killing. The aim of this study is to investigate the relationships between measures of attitudes towards the justification of killing and homicide rates in diverse international populations. METHODS: Four studies assessed variations in acceptance of killing among adults and young people in nineteen nations and four areas in the USA. Study 1 (1996-1997) assessed adult attitudes in Brazil, Chile, Costa Rica, Spain, and Venezuela with personal interviews in major cities. Study 2 (1999-2001) assessed attitudes among high school students in Denmark, Finland, Malta, Mexico, Netherlands, Slovenia, Sweden, Taiwan, and the UK with paper surveys administered in classrooms. Study 3 (2001) used telephone interviews to measure the equivalent attitudes among the US samples nationally and from regions in Texas. Study 4 (2002-2003) used paper surveys in classrooms to measure attitudes among high school students in Armenia, Belarus, Georgia, and the Russian Federation. RESULTS: The acceptance of killing varied significantly among genders and national/regional groups. The mean attitude scores in the four studies combined were significantly correlated with national/regional homicide rates and the amount of variance explained was similar to that for social inequality (GINI). Together the attitude scores and GINI explained 65% of the variance in homicide rates. CONCLUSION: This study provides evidence that variations in attitudes toward the justification of killing may be related to international differences in homicide rates.

Vibratory songs of hybrids from Brazilian and Slovenian populations of the green stink bug Nezara viridula.

Males and females of the green stink bug Nezara viridula produce species and sex specific vibrational signals. The songs of bugs from geographically isolated population differ in their temporal characteristics. Hybrids were produced between the bugs from Brazilian and Slovenian populations in order to examine the levels of genetic inheritance of their vibratory songs. Hybrid males and females produced songs which are distinctly different from parental songs and these differences can be attributable to genetic factor. The results show that in some parameters the hybrid songs are intermediate between the parental types. Several song parameters are apparently sex-linked. It remains to be established whether observed genetically determined differences in vibratory songs also indicate that cryptic species exist within the taxon N. viridula.