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1.
Braz Oral Res ; 32(suppl 1): e76, 2018 Oct 18.
Article in English | MEDLINE | ID: mdl-30365617

ABSTRACT

Endodontic treatment is a common dental procedure used for treating teeth which the pulp tissue has become irreversibly inflamed or necrotic as a result of the carious process or dental trauma. This procedure which involves mechanical and chemical preparation of root canal may affect several mechanical and physical properties of the tooth structure. The endodontic treatment can also influence the longevity of the rehabilitation of endodontically treated teeth and biomechanics during the oral function. For restoring endodontically treated teeth several factor and clinical decisions should be observed. The decision of the fiberglass post usage and the restorative materials are related to several factors such as the quantity and quality of remaining dental structure, presence of ferrule, post cementation length and final coronal restoration. In this review, the authors will address the effect of the endodontic treatment procedures on canal shape and mechanical properties of a tooth, and also discuss the parameters and the biomechanical principles of root canal treated teeth.


Subject(s)
Dental Restoration, Permanent/methods , Post and Core Technique , Root Canal Filling Materials/therapeutic use , Root Canal Therapy/methods , Tooth, Nonvital/therapy , Biomechanical Phenomena , Dental Stress Analysis , Dentin/chemistry , Dentin/pathology , Glass , Humans , Root Canal Therapy/instrumentation , Tooth, Nonvital/pathology , Treatment Outcome
2.
J Appl Oral Sci ; 26: e20170313, 2018.
Article in English | MEDLINE | ID: mdl-29742252

ABSTRACT

Objective To evaluate the effect of restorative strategy (fiber post vs cast post and core), coronal height (0 mm vs 2 mm) and thickness (higher than 1 mm vs lower than 1 mm) on survival rate, fracture resistance and stress distribution. Material and Methods Seventy-two bovine teeth were cleaned and allocated in six groups (n = 12). Twenty-four teeth were sectioned at 13 mm length (no remaining coronal structure) and forty-eight were sectioned at 15 mm (2 mm remaining coronal structure). Half of the forty-eight had remaining coronal thickness lower than 1 mm and the other half had thickness higher than 1 mm. All root canals were prepared at 10 mm (luting length), fiber posts were cemented in thirty-six specimens and cast post and core in other thirty-six. All teeth were restored with metallic crowns. Specimens were submitted to 1.5 million cycles (100 N, 45°, 10 Hz at 2 mm below incisal edge) and evaluated at each 500,000 cycles to detect failures. Specimens that survived were submitted to load to fracture test. Bidimensional (Rhinoceros® 4.0) models were obteined survival data submitted to Kaplan-Meier (α=0.05) analysis and load to fracture values submitted to ANOVA and Tukey tests (α=0.05). Results Groups without remaining coronal structure showed survival rates lower than other groups (p=0.001). ANOVA showed higher values of load to fracture for groups with coronal thickness higher than 1 mm (p=0.0043). Finite element analysis showed better stress distribution in groups with remaining coronal structure and restored with fiber post. Conclusion Specimens without remaining coronal structure have lower survival rates. Specimens with remaining structure lower than 1 mm and without coronal structure support the same load to fracture value independently of the restorative strategy.


Subject(s)
Dental Restoration Failure , Dental Restoration, Permanent/methods , Tooth Fractures/etiology , Tooth, Nonvital/pathology , Tooth, Nonvital/therapy , Analysis of Variance , Animals , Biomechanical Phenomena , Cattle , Crowns , Dental Prosthesis Design , Dental Stress Analysis , Finite Element Analysis , Post and Core Technique , Reference Standards , Reference Values , Tensile Strength , Weight-Bearing
3.
Braz Oral Res ; 32: e7, 2018 Mar 01.
Article in English | MEDLINE | ID: mdl-29513885

ABSTRACT

The aim of this study is to evaluate the action of paramonochlorophenol associated with Furacin followed by calcium hydroxide (CH) dressing in the control of inflammatory root resorption in cases of immediate tooth replantation with delayed endodontic treatment. A total of 28 incisors of 3 male dogs were extracted and replanted after 15 minutes, and randomly divided into 3 groups: Group I (n = 8) - endodontic treatment was performed before the extraction and replantation; Group II (n = 10) - endodontic treatment was performed 30 days after replantation and the root canal was filled with CH dressing; Group III (n = 10) - endodontic treatment was performed 30 days after replantation and root canals received temporary medication of paramonochlorophenol-Furacin followed by CH dressing. The animals were euthanized 90 days after replantation. The histomorphological events analyzed at the epithelial reattachment site were the intensity and extent of acute and chronic inflammatory processes, periodontal ligament (PDL) organization, the intensity and extent of acute and chronic inflammatory processes in the PDL space, root resorption, bone tissue, and ankylosis. Data were submitted to the Wilcoxon Signed Ranks Test for group comparison (α = 5%). In Groups I, II and III the periodontal ligament was regenerated and most of the resorption areas were repaired by newly formed cementum. The depth and extent of root resorption were significantly higher in Group II than in Group III. The use of paramonochlorophenol-furacin followed by CH dressing was more effective in controlling inflammatory root resorption after immediate tooth replantation.


Subject(s)
Anti-Infective Agents, Local/pharmacology , Chlorophenols/pharmacology , Nitrofurazone/pharmacology , Root Resorption/prevention & control , Tooth Replantation/methods , Tooth, Nonvital/drug therapy , Animals , Calcium Hydroxide/pharmacology , Dogs , Male , Medical Illustration , Periodontal Ligament/drug effects , Periodontal Ligament/pathology , Random Allocation , Reproducibility of Results , Root Canal Filling Materials/pharmacology , Root Canal Therapy/methods , Root Resorption/pathology , Time Factors , Tooth Root/drug effects , Tooth Root/pathology , Tooth, Nonvital/pathology , Treatment Outcome
4.
Braz. oral res. (Online) ; 32(supl.1): e76, 2018. graf
Article in English | LILACS | ID: biblio-974469

ABSTRACT

Abstract: Endodontic treatment is a common dental procedure used for treating teeth which the pulp tissue has become irreversibly inflamed or necrotic as a result of the carious process or dental trauma. This procedure which involves mechanical and chemical preparation of root canal may affect several mechanical and physical properties of the tooth structure. The endodontic treatment can also influence the longevity of the rehabilitation of endodontically treated teeth and biomechanics during the oral function. For restoring endodontically treated teeth several factor and clinical decisions should be observed. The decision of the fiberglass post usage and the restorative materials are related to several factors such as the quantity and quality of remaining dental structure, presence of ferrule, post cementation length and final coronal restoration. In this review, the authors will address the effect of the endodontic treatment procedures on canal shape and mechanical properties of a tooth, and also discuss the parameters and the biomechanical principles of root canal treated teeth.


Subject(s)
Humans , Root Canal Filling Materials/therapeutic use , Root Canal Therapy/methods , Post and Core Technique , Tooth, Nonvital/therapy , Dental Restoration, Permanent/methods , Root Canal Therapy/instrumentation , Biomechanical Phenomena , Treatment Outcome , Tooth, Nonvital/pathology , Dental Stress Analysis , Dentin/pathology , Dentin/chemistry , Glass
5.
Braz. oral res. (Online) ; 32: e7, 2018. tab, graf
Article in English | LILACS | ID: biblio-889497

ABSTRACT

Abstract The aim of this study is to evaluate the action of paramonochlorophenol associated with Furacin followed by calcium hydroxide (CH) dressing in the control of inflammatory root resorption in cases of immediate tooth replantation with delayed endodontic treatment. A total of 28 incisors of 3 male dogs were extracted and replanted after 15 minutes, and randomly divided into 3 groups: Group I (n = 8) - endodontic treatment was performed before the extraction and replantation; Group II (n = 10) - endodontic treatment was performed 30 days after replantation and the root canal was filled with CH dressing; Group III (n = 10) - endodontic treatment was performed 30 days after replantation and root canals received temporary medication of paramonochlorophenol-Furacin followed by CH dressing. The animals were euthanized 90 days after replantation. The histomorphological events analyzed at the epithelial reattachment site were the intensity and extent of acute and chronic inflammatory processes, periodontal ligament (PDL) organization, the intensity and extent of acute and chronic inflammatory processes in the PDL space, root resorption, bone tissue, and ankylosis. Data were submitted to the Wilcoxon Signed Ranks Test for group comparison (α = 5%). In Groups I, II and III the periodontal ligament was regenerated and most of the resorption areas were repaired by newly formed cementum. The depth and extent of root resorption were significantly higher in Group II than in Group III. The use of paramonochlorophenol-furacin followed by CH dressing was more effective in controlling inflammatory root resorption after immediate tooth replantation.


Subject(s)
Animals , Male , Dogs , Root Resorption/prevention & control , Tooth Replantation/methods , Chlorophenols/pharmacology , Tooth, Nonvital/drug therapy , Anti-Infective Agents, Local/pharmacology , Nitrofurazone/pharmacology , Periodontal Ligament/drug effects , Periodontal Ligament/pathology , Root Canal Filling Materials/pharmacology , Root Canal Therapy/methods , Root Resorption/pathology , Time Factors , Tooth Root/drug effects , Tooth Root/pathology , Calcium Hydroxide/pharmacology , Random Allocation , Reproducibility of Results , Treatment Outcome , Tooth, Nonvital/pathology , Medical Illustration
6.
J. appl. oral sci ; J. appl. oral sci;26: e20170313, 2018. tab, graf
Article in English | LILACS, BBO - Dentistry | ID: biblio-893683

ABSTRACT

Abstract Objective To evaluate the effect of restorative strategy (fiber post vs cast post and core), coronal height (0 mm vs 2 mm) and thickness (higher than 1 mm vs lower than 1 mm) on survival rate, fracture resistance and stress distribution. Material and Methods Seventy-two bovine teeth were cleaned and allocated in six groups (n = 12). Twenty-four teeth were sectioned at 13 mm length (no remaining coronal structure) and forty-eight were sectioned at 15 mm (2 mm remaining coronal structure). Half of the forty-eight had remaining coronal thickness lower than 1 mm and the other half had thickness higher than 1 mm. All root canals were prepared at 10 mm (luting length), fiber posts were cemented in thirty-six specimens and cast post and core in other thirty-six. All teeth were restored with metallic crowns. Specimens were submitted to 1.5 million cycles (100 N, 45°, 10 Hz at 2 mm below incisal edge) and evaluated at each 500,000 cycles to detect failures. Specimens that survived were submitted to load to fracture test. Bidimensional (Rhinoceros® 4.0) models were obteined survival data submitted to Kaplan-Meier (α=0.05) analysis and load to fracture values submitted to ANOVA and Tukey tests (α=0.05). Results Groups without remaining coronal structure showed survival rates lower than other groups (p=0.001). ANOVA showed higher values of load to fracture for groups with coronal thickness higher than 1 mm (p=0.0043). Finite element analysis showed better stress distribution in groups with remaining coronal structure and restored with fiber post. Conclusion Specimens without remaining coronal structure have lower survival rates. Specimens with remaining structure lower than 1 mm and without coronal structure support the same load to fracture value independently of the restorative strategy.


Subject(s)
Animals , Cattle , Tooth Fractures/etiology , Tooth, Nonvital/pathology , Tooth, Nonvital/therapy , Dental Restoration Failure , Dental Restoration, Permanent/methods , Reference Standards , Reference Values , Tensile Strength , Biomechanical Phenomena , Analysis of Variance , Post and Core Technique , Weight-Bearing , Dental Prosthesis Design , Finite Element Analysis , Crowns , Dental Stress Analysis
7.
J Contemp Dent Pract ; 16(2): 147-53, 2015 02 01.
Article in English | MEDLINE | ID: mdl-25906807

ABSTRACT

PURPOSE: The purpose of this study was to evaluate the effect of self-adhesive and self-etching resin cements on the bond strength of nonmetallic posts in different root regions. MATERIALS AND METHODS: Sixty single-rooted human teeth were decoronated, endodontically treated, post-space prepared, and divided into six groups. Glass-fiber (GF) posts (Exacto, Angelus) and fiber-reinforced composite (FRC) posts (EverStick, StickTeck) were cemented with self-adhesive resin cement (Breeze) (SA) (Pentral Clinical) and self-etching resin cement (Panavia-F) (SE) (Kuraray). Six 1-mm-thick rods were obtained from the cervical (C), middle (M), and apical (A) regions of the roots. The specimens were then subjected to microtensile testing in a special machine (BISCO; Schaumburg, IL, USA) at a crosshead speed of 0.5 mm/min. Microtensile bond strength data were analyzed with two-way ANOVA and Tukey's tests. RESULTS: Means (and SD) of the MPa were: GF/SA/C: 14.32 (2.84), GF/SA/M: 10.69 (2.72), GF/SA/A: 6.77 (2.17), GF/SE/C: 11.56 (4.13), GF/SE/M: 6.49 (2.54), GF/SE/A: 3.60 (1.29), FRC/SA/C: 16.89 (2.66), FRC/SA/M: 13.18 (2.19), FRC/SA/A: 8.45 (1.77), FRC/SE/C: 13.69 (3.26), FRC/SE/M: 9.58 (2.23), FRC/SE/A: 5.62 (2.12). The difference among the regions was statistically significant for all groups (p < 0.05). The self-adhesive resin cement showed better results than the self-etching resin cement when compared to each post (p < 0.05). No statistically significant differences in bond strengths of the resin cements when comparable to each post (p > 0.05). CONCLUSION: The bond strength values were significantly affected by the resin cement and the highest values were found for self-adhesive resin cement.


Subject(s)
Dental Bonding/methods , Dental Pulp Cavity/ultrastructure , Post and Core Technique/instrumentation , Resin Cements/chemistry , Cementation/methods , Composite Resins/chemistry , Dental Materials/chemistry , Dental Stress Analysis/instrumentation , Glass/chemistry , Humans , Materials Testing , Microscopy, Electron, Scanning , Random Allocation , Root Canal Preparation/methods , Stress, Mechanical , Surface Properties , Tensile Strength , Tooth Apex/ultrastructure , Tooth Cervix/ultrastructure , Tooth, Nonvital/pathology
8.
Indian J Dent Res ; 25(5): 576-9, 2014.
Article in English | MEDLINE | ID: mdl-25511054

ABSTRACT

AIM: This study evaluated the efficiency and effectiveness of three glass fiber post removal techniques. MATERIALS AND METHODS: Forty-five extracted maxillary teeth were endodontically treated and cross-sectioned in thirds. Presence of cementing agent and dental structure wear were assessed by analyzing images taken before luting of glass fiber post and after removal procedure. Teeth were divided into 3 groups: Group 1 - diamond bur + Largo reamer; Group 2 - ultrasonic insert; Group 3 - carbide bur + ultrasonic insert. Time spent on removal procedures, dental structure wear and amount of remaining cement agent were recorded and results submitted to ANOVA, Kruskal Wallis and Tukey-Kramer tests. RESULTS: Group 1 - 16'46", 33.33% and 6.99%; Group 2 - 12'31", 40% and 7.86%; and Group 3 - 10'24", 80% and 8.14%. Group 3 presented the most effective removal of glass fiber posts. CONCLUSION: There was no significant difference in efficiency among the evaluated techniques.


Subject(s)
Dental Debonding/methods , Dental Prosthesis Design , Glass/chemistry , Post and Core Technique/instrumentation , Acid Etching, Dental/methods , Dental Debonding/instrumentation , Dental Pulp Cavity/pathology , Diamond/chemistry , Humans , Materials Testing , Random Allocation , Resin Cements/chemistry , Root Canal Preparation/instrumentation , Surface Properties , Time Factors , Tooth, Nonvital/pathology , Ultrasonic Therapy/instrumentation , Ultrasonic Waves
9.
J Endod ; 40(8): 1201-5, 2014 Aug.
Article in English | MEDLINE | ID: mdl-25069933

ABSTRACT

INTRODUCTION: Luting posts inside the root canal is still a challenge because of the difficulty of bonding adhesive materials in the apical third of roots. This study evaluated the effect of the application mode of 3 simplified etch-and-rinse adhesives on the push-out bond strength (PBS), nanoleakage (NL), and in situ degree of conversion (DC) of fiber posts in the root canal. METHODS: The roots of human premolars were endodontically prepared and divided into 6 groups according to the combination of the main factors: adhesive (Ambar, FGM, Joinville, SC, Brazil; Adper Single Bond 2, 3MESPE, St Paul, MN; and XP Bond+self-cure activator, DeTrey Dentsply, Konstanz, Germany) and application mode (manual or sonic). The posts were cemented and the PBS tested at 0.5 mm/min. The NL was evaluated by scanning electron microscopy after the immersion of specimens in 50% silver nitrate. Micro-Raman spectroscopy was used to measure the in situ DC. Root third was also considered in the statistical evaluation. Data were analyzed by 3-way repeated measures analysis of variance and Tukey tests (5%). RESULTS: Under sonic application, the PBS and the in situ DC increased, whereas NL decreased significantly for all groups in the middle and apical thirds (P < .05). CONCLUSIONS: The application of simplified adhesives by sonic mode in the root canal is a feasible tool to increase the fiber post bond to root canals.


Subject(s)
Dental Bonding/methods , Dental Pulp Cavity/ultrastructure , Dentin-Bonding Agents/chemistry , Post and Core Technique/instrumentation , Resin Cements/chemistry , Sonication/methods , Bisphenol A-Glycidyl Methacrylate/chemistry , Cementation/methods , Dental Cements/chemistry , Dental Leakage/classification , Dental Stress Analysis/instrumentation , Humans , Materials Testing , Methacrylates/chemistry , Microscopy, Electron, Scanning , Polyethylene Glycols/chemistry , Polymerization , Polymethacrylic Acids/chemistry , Random Allocation , Root Canal Preparation/methods , Silver Staining/methods , Spectrum Analysis, Raman , Stress, Mechanical , Tooth Apex/ultrastructure , Tooth, Nonvital/pathology
10.
J Adhes Dent ; 15(3): 269-74, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23534023

ABSTRACT

PURPOSE: To evaluate the effects of different glass-fiber post surface treatments on the bond strength to root dentin. MATERIALS AND METHODS: Fifty bovine incisors were used in this study. After removing the crowns, the teeth were endodontically treated. The roots were randomly divided into five groups according to post surface treatment. The groups were as follows: CO (Control) - no treatment; G1 - RelyX Ceramic Primer (silane) only; G2 - silane and Solobond M; G3 - silane and Scotchbond Adhesive; G4 - silane and Excite. For post cementation, RelyX Unicem was used according to the manufacturer's recommendation and the roots were stored in a light-proof container with 100% relative humidity for 24 h. The specimens were transversally sectioned. Subsequently, the cervical, middle, and apical regions of the root were positioned in a push-out device and tested at 0.5 mm/min using a universal testing machine (Instron). The data were statistically analyzed with two-way ANOVA and Tukey's post-hoc test. The fractured specimens were then observed under a stereoscopic loupe at 60X magnification. RESULTS: No significant difference in bond strength was found among the groups that received a silane or silane plus an adhesive system (p > 0.05). However, the CO (no silane) showed the lowest bond strength. Regarding G1, G2, G3, and G4, the cervical region of the root canal attained better bond strengths than did the middle or apical regions. The most frequent failure mode occurred at the cement/dentin interface. CONCLUSION: Silane application may be necessary to improve the adhesion of fiber posts luted with the self-adhesive resin cement evaluated here. The application of an adhesive layer between the fiber post and resin cement did not have any influence on the bond strength when the silane coupling was previously used.


Subject(s)
Dental Bonding , Glass/chemistry , Post and Core Technique/instrumentation , Resin Cements/chemistry , Silanes/chemistry , Animals , Cattle , Cementation/methods , Dental Pulp Cavity/ultrastructure , Dental Stress Analysis/instrumentation , Dentin/ultrastructure , Dentin-Bonding Agents/chemistry , Humidity , Materials Testing , Methacrylates/chemistry , Microscopy, Electron, Scanning , Random Allocation , Stress, Mechanical , Surface Properties , Time Factors , Tooth Apex/ultrastructure , Tooth Cervix/ultrastructure , Tooth, Nonvital/pathology
11.
J Dent ; 41 Suppl 3: e93-6, 2013 Aug.
Article in English | MEDLINE | ID: mdl-23123496

ABSTRACT

OBJECTIVES: One of the goals of endodontic treatment is the adequate filling of the root canal,which is often done using gutta-percha and sealer. It has been reported that sealer remnants in the coronary pulp chamber cause tooth color changes. Therefore, this study was designed to examine the effect of endodontic sealer remnants on tooth color, testing the hypothesis that sealers cause coronal color changes. METHODS: Forty single-rooted human teeth were endodontically treated leaving excess sealer material in the coronary pulp chamber. The specimens were divided into four groups (n = 10) according to the endodontic sealer used (AH, AH Plus; EF, Endofill; EN,endome´ thasoneN; and S26, Sealer 26). Teeth were stored at 37 8C moist environment.Color coordinates (L*a*b*) were measured with a spectrophotometer before endodontic treatment(baseline-control), 24 h and 6 months after treatment. L*a*b* values were used to calculate color changes (DE). Data were statistically analyzed using Kruskal­Wallis and Mann­Whitney-U tests. RESULTS: Color changes were observed for all groups with S26 and EN producing the greatest mean DE values after 6 months. CONCLUSION: Endodontic sealer remnants affect tooth color confirming the experimental hypothesis. CLINICAL SIGNIFICANCE: This study examined the effect of endodontic sealer remnants on tooth color, and observed that after 6 months, the sealers produced unacceptable color changes.


Subject(s)
Root Canal Filling Materials/adverse effects , Tooth Discoloration/chemically induced , Tooth/drug effects , Bismuth/adverse effects , Calcium Hydroxide/adverse effects , Color , Dexamethasone/adverse effects , Drug Combinations , Epoxy Resins/adverse effects , Formaldehyde/adverse effects , Humans , Humidity , Hydrocortisone/adverse effects , Materials Testing , Spectrophotometry/methods , Temperature , Thymol/adverse effects , Thymol/analogs & derivatives , Time Factors , Tooth/pathology , Tooth, Nonvital/pathology
12.
J Oral Sci ; 54(1): 127-31, 2012 Mar.
Article in English | MEDLINE | ID: mdl-22466897

ABSTRACT

Endodontic treatment of teeth with perforating internal root resorption represents a clinical challenge. In most cases, extraction of the tooth and subsequent replacement with an osseointegrated implant is indicated. Presented herein is a case report of a maxillary lateral incisor with advanced perforating internal root resorption in the middle third of the root and the presence of a sinus tract. Mineral Trioxide Aggregate (MTA) was used with the aid of a surgical microscope in order to fill the resorption area after conventional root canal therapy of the apical segment. At the follow-up after 11 years and 8 months, the patient was clinically asymptomatic and the sinus tract had disappeared. The radiographic examination and computerized tomography indicated periodontal bone repair.


Subject(s)
Root Canal Filling Materials/therapeutic use , Root Resorption/therapy , Adult , Aluminum Compounds/therapeutic use , Calcium Compounds/therapeutic use , Dental Fistula/therapy , Drug Combinations , Female , Humans , Incisor/pathology , Maxilla , Oxides/therapeutic use , Retreatment , Root Canal Therapy/methods , Root Resorption/pathology , Silicates/therapeutic use , Tooth, Nonvital/pathology
13.
J Endod ; 38(1): 91-4, 2012 Jan.
Article in English | MEDLINE | ID: mdl-22152628

ABSTRACT

INTRODUCTION: The endodontic regenerative procedure (ERP), which is an alternative to calcium hydroxide-induced apexification, involves the use of a triple antibiotic paste (TAP) as a dressing material. The aim of this study was to evaluate the response of rat subcutaneous tissue to implanted polyethylene tubes that were filled with TAP or calcium hydroxide. METHODS: Thirty rats received 2 individual implants of polyethylene tubes filled with TAP or calcium hydroxide paste (CHP) and another empty tube as a control. Thirty additional rats received 2 individual implants consisting of polyethylene tubes filled with dressing material carriers (macrogol and propylene glycol) and a sham procedure. After 7, 15, 30, 60, and 90 days, 12 animals were euthanized, and the tubes and surrounding tissue were removed and processed for histology by using glycol methacrylate and stained with hematoxylin and eosin. The histological score ranged from 0 to 3 depending on the content of inflammatory cells; the fibrous capsule was considered thin or thick, and necrosis and calcification were recorded as present or absent. The results were analyzed using the Kruskal-Wallis test. RESULTS: Both dressing materials induced moderate reactions at 7 and 15 days. These reactions were similar to the control (P > .05) and reduced in intensity (to mild) from day 30 onward (P > .05). The carriers did not interfere with the reaction of the dressing materials. CONCLUSIONS: TAP and CHP were biocompatible over the different experimental periods examined.


Subject(s)
Anti-Bacterial Agents/pharmacology , Apexification/methods , Biocompatible Materials/pharmacology , Root Canal Filling Materials/pharmacology , Animals , Anti-Bacterial Agents/administration & dosage , Calcinosis/pathology , Calcium Hydroxide/pharmacology , Ciprofloxacin/administration & dosage , Ciprofloxacin/pharmacology , Drug Carriers , Drug Combinations , Fibrosis , Lymphocytes/pathology , Macrophages/pathology , Male , Materials Testing , Metronidazole/administration & dosage , Metronidazole/pharmacology , Minocycline/administration & dosage , Minocycline/pharmacology , Necrosis , Polyethylene Glycols , Rats , Rats, Wistar , Regeneration/drug effects , Subcutaneous Tissue/drug effects , Subcutaneous Tissue/pathology , Time Factors , Tooth, Nonvital/pathology
14.
Int. j. odontostomatol. (Print) ; 6(3): 291-295, 2012. ilus
Article in English | LILACS | ID: lil-676188

ABSTRACT

Previous studies indicate that dental tissues are a source of mitochondrial DNA that could be useful for human identification. The main cell in the pulpo-dentin complex is the odontoblast, whose cellular body is located on the border between the pulp and dentin and continues through cell processes. In endodontically treated teeth, pulp tissue is removed, assuming the complete elimination of cellular content and the inner third of dentin. Facing the possibility of finding teeth that were treated endodontically as the only source available for a forensic analysis, is that the objective of this study is to determine the presence of cellular debris in the dentin of teeth with root canal treatment. Twenty teeth roots obtained from 8 single and multi-rooted teeth were treated endodontically, with conventional manual technique. The samples were processed by conventional histological analysis (H&E). In root canals endodontic cement remnants and cylinder-cubic structures resembled odontoblastic bodies were observed, but without certainty to establish its presence. This research concludes that it is not possible to determine presence of cellular debris in endodontically treated teeth using the described technique...


Estudios previos indican que los tejidos dentales son fuente de DNA mitocondrial útiles para la identificación humana. La principal célula del complejo pulpo-dentinario es el odontoblasto, cuyo cuerpo celular ubicado en el límite entre la pulpa y la dentina se continúa por prolongaciones celulares. En dientes tratados endodónticamente se extrae el tejido pulpar, presumiendo la completa eliminación del contenido celular y el tercio interno de la dentina. Frente a la posibilidad de encontrar dientes que fueron tratados endodónticamente como única fuente disponible para análisis forense, es que el objetivo de este estudio es determinar la presencia de restos celulares en la dentina de dientes con tratamiento de canales radiculares. 20 raíces dentarias obtenidas de 8 dientes uni y multirradiculares, fueron tratadas endodónticamente con terapia manual convencional. Las muestras fueron procesadas mediante análisis histológico convencional (H&E). En los canales radiculares se observaron restos de cemento endodóntico y estructuras cilindro-cúbicas que asemejaron a cuerpos de odontoblastos, sin poder establecer con certeza su presencia. En esta investigación se concluye que no es posible determinar mediante la técnica utilizada la presencia de restos celulares en dientes tratados endodónticamente...


Subject(s)
Humans , DNA, Mitochondrial , Tooth, Nonvital/pathology , Odontoblasts , Root Canal Therapy , Forensic Dentistry , Pilot Projects
15.
J Endod ; 37(8): 1069-73, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21763896

ABSTRACT

INTRODUCTION: The present study aimed at developing an experimental model in rat molars for evaluating treatment strategies in necrotic immature teeth. METHODS: To define the periods to be adopted in the experimental procedures and to confirm induction of periapical lesions and interruption of root embryogenesis, the left lower first molars of 4-weeks-old Wistar rats underwent pulpectomy and were left open to the oral environment. Comparisons with the right lower first molars (vital teeth) were performed in animals with ages of 7, 10, 13, and 16 weeks. In another group of animals the teeth were left open for 3 weeks, and then interventions for disinfection including the use of an antibiotic paste were carried out. Root formation was then assessed after 3 and 6 weeks on the basis of radiographic and histologic evaluation. RESULTS: Vital teeth showed increase of root length and hard tissue thickness throughout the experimental periods. On the other hand, induction of necrosis arrested root formation. Teeth subjected to disinfection with sodium hypochlorite associated with the triple antibiotic paste showed significant reduction of periapical lesions, gain in root length, and increased wall thickness compared with the control (P < .05). CONCLUSIONS: The root canal disinfection protocol used was able to reduce periapical lesion size and improve root development. The experimental model presented should contribute to studies that aim at improving therapeutic strategies for necrotic immature teeth by using a rat model.


Subject(s)
Anti-Bacterial Agents/therapeutic use , Apexification , Dental Pulp Necrosis/drug therapy , Root Canal Irrigants/therapeutic use , Tooth Apex/growth & development , Animals , Cementogenesis , Ciprofloxacin/therapeutic use , Dental Pulp Necrosis/complications , Dental Pulp Necrosis/physiopathology , Disease Models, Animal , Drug Combinations , Metronidazole/therapeutic use , Minocycline/therapeutic use , Odontogenesis , Periapical Periodontitis/etiology , Radiography , Rats , Rats, Wistar , Sodium Hypochlorite/therapeutic use , Tooth Apex/diagnostic imaging , Tooth, Nonvital/diagnostic imaging , Tooth, Nonvital/pathology
16.
Dent Traumatol ; 27(3): 184-8, 2011 Jun.
Article in English | MEDLINE | ID: mdl-21342436

ABSTRACT

Pulse oximetry (PO) is a well-accepted non-invasive method for assessing vascular health, based on oxygen saturation (SaO(2) ) measurements. The objectives of this study were to design and build a custom-made PO dental sensor holder, to evaluate the effectiveness of PO in determining pulp blood flow in primary and permanent teeth, and to compare the SaO(2) levels obtained in teeth and on the little finger of patients. The PO sensor adapted to the custom-made sensor holder is termed as a device to position and hold the PO sensor. This study evaluated SaO(2) readings obtained in the pulp of 123 teeth of 84 children aged 4-13years. The teeth were divided into three groups: group I - primary teeth: 23 central incisors and 28 canines; group II - permanent teeth: 25 central incisors and 28 canines; and group III - endodontically treated teeth (control): 12 permanent central incisors and seven primary central incisors. The pulp blood flow and SaO(2) were measured and were compared with readings of the patient's finger. Data were analyzed by paired t-tests and Pearson's analysis method. The PO was able to identify all the clinically normal pulps contained in the sample, and all the endodontically treated teeth (controls) showed no response. The mean SaO(2) values were 85.27% in the teeth and 92.85% in the fingers. In conclusion, PO readings were effective in determining pulp blood flow in primary and permanent teeth. However, there was no correlation between the SaO(2) values in the fingers and in the teeth of the patients (P<0.05).


Subject(s)
Dental Pulp/blood supply , Oximetry/methods , Tooth, Deciduous/blood supply , Adolescent , Child , Child, Preschool , Cuspid/blood supply , Equipment Design , Fingers/blood supply , Humans , Incisor/blood supply , Oximetry/instrumentation , Oxygen/blood , Regional Blood Flow/physiology , Time Factors , Tooth, Nonvital/pathology
17.
J Prosthodont ; 19(5): 374-80, 2010 Jul.
Article in English | MEDLINE | ID: mdl-20202103

ABSTRACT

PURPOSE: To evaluate the effects of the elapsed time (ET) after nonvital bleaching (NVB) and sodium ascorbate application (10%) (SAA) on the shear bond strength of dentin to ceramic. MATERIALS AND METHODS: Bovine incisors were selected, internally bleached (35% carbamide peroxide) for 9 days and submitted to the following treatments (n = 10): G1, G2, G3-luting after 1, 7, and 14 days; G4, G5, and G6-luting after SAA, 1, 7, and 14 days, respectively. G7 and G8 were not bleached: G7-luting 24 hours after access cavity sealing; G8-luting 24 hours after access cavity sealing after SAA. After NVB, the vestibular dentin was exposed and flattened. The SAA was applied to the dentin (G4, G5, G6, G8) for 10 minutes, and it was then washed and dried. The dentin was etched (37% phosphoric acid), and an adhesive system (Single Bond 2) was applied. Feldspathic ceramic discs (VM7; 4-mm diameter, 3-mm thick) were luted with a dual-resin agent (RelyX ARC, 3M ESPE Dental Products, St. Paul, MN). After 24 hours, specimens were submitted to shear test on a universal testing machine. The data (MPa) were submitted to ANOVA and Dunnet's test (5%). RESULTS: The means (+/- SD) obtained were (MPa): G1 (14 +/- 4.5), G2 (14.6 +/- 3.1), G3 (14 +/- 3.7), G4 (15.5 +/- 4.6), G5 (19.87 +/- 4.5), G6 (16.5 +/- 3.7), G7 (22.8 +/- 6.2), and G8 (18.9 +/- 5.4). SAA had a significant effect on bond strength (p= 0.0054). The effect of ET was not significant (p= 0.1519). G5 and G6 presented higher values than the other bleached groups (p < 0.05) and similar to G7 and G8 (p > 0.05). CONCLUSIONS: After NVB, adhesive luting to dentin is recommended after 7 days if sodium ascorbate has been applied prior to dentin hybridization.


Subject(s)
Antioxidants/chemistry , Ascorbic Acid/chemistry , Cementation/methods , Dental Bonding , Dental Porcelain/chemistry , Dentin/ultrastructure , Tooth Bleaching/methods , Acid Etching, Dental/methods , Adhesiveness , Aluminum Silicates/chemistry , Animals , Bisphenol A-Glycidyl Methacrylate/chemistry , Carbamide Peroxide , Cattle , Dental Cements/chemistry , Dental Pulp Cavity/ultrastructure , Dentin-Bonding Agents/chemistry , Hydrofluoric Acid/chemistry , Materials Testing , Oxidants/chemistry , Peroxides/chemistry , Phosphoric Acids/chemistry , Polyethylene Glycols/chemistry , Polymethacrylic Acids/chemistry , Potassium Compounds/chemistry , Random Allocation , Resin Cements/chemistry , Shear Strength , Stress, Mechanical , Time Factors , Tooth, Nonvital/pathology , Urea/analogs & derivatives , Urea/chemistry
18.
J Dent Res ; 89(6): 587-91, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20348486

ABSTRACT

The clinical success of fiber posts has been attributed to their lower elastic modulus. The tested hypothesis was that fiber posts could lead to lower risk of post debonding and lower risk of root fracture, despite an increase in root stresses. Stress analyses were carried out with a 3D finite element model of a premolar restored with a metallic or a fiber post. Bonded and non-bonded post/cement interface conditions were simulated. We calculated risk-of-fracture indices by determining the highest principal stress values divided by the tensile strength. Shear stresses along the post/cement interface were analyzed for the bonded models. Compared with the premolar restored with a metallic post, the fiber post generated lower stresses along the interface and higher stresses in the root. However, with the fiber post, fracture was less likely to occur in the root, since its core and post fracture indices were higher.


Subject(s)
Dental Materials/chemistry , Dental Prosthesis Design , Glass/chemistry , Post and Core Technique/instrumentation , Tooth Fractures/prevention & control , Tooth Root/physiology , Bicuspid/pathology , Biomechanical Phenomena , Composite Resins/chemistry , Computer Simulation , Dental Alloys/chemistry , Dental Bonding , Dental Stress Analysis , Elastic Modulus , Finite Element Analysis , Humans , Imaging, Three-Dimensional , Materials Testing , Models, Biological , Resin Cements/chemistry , Risk Factors , Shear Strength , Stress, Mechanical , Tensile Strength , Tooth Root/injuries , Tooth, Nonvital/pathology
19.
J Appl Oral Sci ; 17(6): 579-83, 2009.
Article in English | MEDLINE | ID: mdl-20027430

ABSTRACT

OBJECTIVE: This study evaluated in vitro the influence of an eugenol-based sealer (EndoFill) on the retention of stainless steel prefabricated posts cemented with zinc phosphate and resin-based (Panavia F) cements after different periods of root canal obturation, using the pull-out test. MATERIAL AND METHODS: Sixty upper canines were decoronated and the roots were embedded in resin blocks. The specimens were distributed into 3 groups, according to the period elapsed between canal obturation and post cementation: Group I - immediately; Group II - 72 h and Group III - 4 months. The groups were subdivided according to the type of cement used for post cementation: A - zinc phosphate and B - Panavia F. Following the experimental periods, specimens were subjected to pullout test in an Instron machine with application of tensile force at a crosshead speed of 0.5 mm/min until post dislodgement. The maximum forces required for post removal were recorded (kN) and means were subjected to statistical analysis by 2-way ANOVA and Tukey-Kramer test (alpha=0.001) RESULTS: There were statistically significant differences (p<0.01) between the posts cemented with zinc phosphate cement (0.2112 kN) and Panavia F (0.0501 kN). However, no statistically significant differences (p>0.05) were found between the three post cementation periods, regardless of the cement. CONCLUSIONS: It was concluded that the eugenol-based sealer influenced the tensile strength of the posts cemented with the resin cement, but had no influence on the time waited between root canal obturation and post space preparation/post cementation.


Subject(s)
Cementation/methods , Dental Bonding , Post and Core Technique/instrumentation , Root Canal Filling Materials/chemistry , Zinc Oxide-Eugenol Cement/chemistry , Cuspid/pathology , Dental Alloys/chemistry , Dental Stress Analysis/instrumentation , Humans , Materials Testing , Resin Cements/chemistry , Root Canal Obturation , Root Canal Preparation , Stainless Steel/chemistry , Stress, Mechanical , Temperature , Tensile Strength , Time Factors , Tooth, Nonvital/pathology , Zinc Phosphate Cement/chemistry
20.
J. appl. oral sci ; J. appl. oral sci;17(6): 579-583, Nov.-Dec. 2009. ilus, tab
Article in English | LILACS | ID: lil-534423

ABSTRACT

OBJECTIVE: This study evaluated in vitro the influence of an eugenol-based sealer (EndoFill) on the retention of stainless steel prefabricated posts cemented with zinc phosphate and resin-based (Panavia F) cements after different periods of root canal obturation, using the pull-out test. MATERIAL AND METHODS: Sixty upper canines were decoronated and the roots were embedded in resin blocks. The specimens were distributed into 3 groups, according to the period elapsed between canal obturation and post cementation: Group I - immediately; Group II - 72 h and Group III - 4 months. The groups were subdivided according to the type of cement used for post cementation: A - zinc phosphate and B - Panavia F. Following the experimental periods, specimens were subjected to pullout test in an Instron machine with application of tensile force at a crosshead speed of 0.5 mm/min until post dislodgement. The maximum forces required for post removal were recorded (kN) and means were subjected to statistical analysis by 2-way ANOVA and Tukey-Kramer test (á=0.001) RESULTS: There were statistically significant differences (p<0.01) between the posts cemented with zinc phosphate cement (0.2112 kN) and Panavia F (0.0501 kN). However, no statistically significant differences (p>0.05) were found between the three post cementation periods, regardless of the cement. CONCLUSIONS: It was concluded that the eugenol-based sealer influenced the tensile strength of the posts cemented with the resin cement, but had no influence on the time waited between root canal obturation and post space preparation/post cementation.


Subject(s)
Humans , Cementation/methods , Dental Bonding , Post and Core Technique/instrumentation , Root Canal Filling Materials/chemistry , Zinc Oxide-Eugenol Cement/chemistry , Cuspid/pathology , Dental Alloys/chemistry , Dental Stress Analysis/instrumentation , Materials Testing , Root Canal Obturation , Root Canal Preparation , Resin Cements/chemistry , Stress, Mechanical , Stainless Steel/chemistry , Temperature , Tensile Strength , Time Factors , Tooth, Nonvital/pathology , Zinc Phosphate Cement/chemistry
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